##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939670_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4229060 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.247948953195273 2.0 2.0 31.0 2.0 33.0 2 22.580413850832098 16.0 16.0 31.0 16.0 34.0 3 27.097267477879246 28.0 25.0 31.0 16.0 34.0 4 32.335278998169805 35.0 32.0 35.0 25.0 37.0 5 32.942469012026315 35.0 33.0 37.0 27.0 37.0 6 33.31844357847843 35.0 33.0 37.0 28.0 37.0 7 33.4812520985751 35.0 33.0 37.0 28.0 37.0 8 33.400688569091 35.0 33.0 37.0 26.0 37.0 9 34.78916496810166 37.0 34.0 39.0 27.0 39.0 10-11 34.627598449773714 37.0 34.0 39.0 27.0 39.0 12-13 34.648743337763 37.0 34.0 39.0 27.0 39.0 14-15 35.38604772691804 38.0 34.0 40.0 27.0 41.0 16-17 34.9872055492237 38.0 33.0 40.0 25.5 41.0 18-19 35.208642582512425 38.0 33.5 40.0 26.0 41.0 20-21 35.187070767499165 38.0 34.0 40.0 26.0 41.0 22-23 35.0033847947298 38.0 33.5 40.0 25.5 41.0 24-25 34.82781079010466 38.0 33.0 40.0 25.0 41.0 26-27 34.9480090138234 38.0 34.0 40.0 25.0 41.0 28-29 34.70673187422264 38.0 33.0 40.0 24.0 41.0 30-31 34.607435103781924 38.0 33.0 40.0 24.0 41.0 32-33 34.32566468671526 38.0 33.0 40.0 24.0 41.0 34-35 34.08968482357782 38.0 33.0 40.0 23.0 41.0 36-37 33.84504925444425 38.0 32.5 40.0 21.0 41.0 38-39 33.664016235286326 38.0 32.0 40.0 19.5 41.0 40-41 33.29919119142315 37.5 31.0 40.0 17.5 41.0 42-43 33.08274226423839 37.0 31.0 40.0 17.5 41.0 44-45 32.78535844844954 37.0 31.0 40.0 15.5 41.0 46-47 32.52421330035516 37.0 30.5 40.0 15.0 41.0 48-49 32.198723238733905 37.0 30.0 39.0 13.0 40.5 50-51 31.55550157718264 35.5 30.0 38.5 10.0 40.0 52-53 31.599621665334613 36.0 30.0 39.0 9.0 40.0 54-55 31.737537064974248 36.0 30.0 39.0 8.5 40.0 56-57 31.453662870708857 36.0 29.5 39.0 8.0 40.0 58-59 31.050724983802546 35.5 29.0 39.0 7.0 40.0 60-61 30.47264829536587 35.0 28.0 38.5 4.0 40.0 62-63 30.085036509295207 34.5 28.0 38.0 2.0 40.0 64-65 29.512990357195214 34.0 27.0 38.0 2.0 40.0 66-67 28.921900729712988 34.0 26.0 37.5 2.0 39.0 68-69 28.575388384179938 34.0 26.0 37.0 2.0 39.0 70-71 28.049387925449153 33.0 26.0 36.0 2.0 39.0 72-73 27.458567979645593 32.5 25.0 36.0 2.0 38.5 74-75 26.896581509839066 32.0 23.5 35.5 2.0 38.0 76-77 26.25532718854781 32.0 22.0 35.0 2.0 37.0 78-79 25.594402183936857 31.0 20.5 34.5 2.0 36.5 80-81 24.859611237485396 31.0 18.5 34.0 2.0 36.0 82-83 24.168019252505285 30.0 15.0 34.0 2.0 35.5 84-85 23.459736797302476 30.0 7.5 34.0 2.0 35.0 86-87 22.781756584205475 29.0 4.5 34.0 2.0 35.0 88-89 21.961551857859668 29.0 2.0 34.0 2.0 35.0 90-91 20.98264117794498 28.0 2.0 33.0 2.0 34.0 92-93 19.901853366942063 26.0 2.0 32.5 2.0 34.0 94-95 18.89658411088989 25.0 2.0 32.0 2.0 34.0 96-97 17.98426683471031 24.5 2.0 32.0 2.0 34.0 98-99 16.909896998387346 20.0 2.0 31.5 2.0 34.0 100-101 15.320536715014685 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.8975462749937 1101 2 8.129532097957494 1101 3 3.754838652295195 1101 4 2.414858802163728 1101 5 1.6686829175752678 1101 6 1.4088732689967571 1101 7 1.0404732209322631 1101 8 1.003526058501798 1101 9 1.0853988109836266 1101 10-11 1.1412537712236244 1101 12-13 0.8318357302954595 1101 14-15 1.0429122932428072 1101 16-17 1.0309233620309826 1101 18-19 1.063011698379995 1101 20-21 1.0553419453990784 1101 22-23 0.9772038765827986 1101 24-25 1.16899263753443 1101 26-27 1.1618353140192639 1101 28-29 1.3022504385808986 1101 30-31 1.3000254001393827 1101 32-33 1.1942715912105157 1101 34-35 1.3475465015076935 1101 36-37 1.2711897576881839 1101 38-39 1.0941614092820302 1101 40-41 1.3256539925788005 1101 42-43 1.2550311269733072 1101 44-45 1.0604421854725672 1101 46-47 0.9275403044266 1101 48-49 0.8669232442168564 1101 50-51 0.9255219894867501 1101 52-53 1.1166827886667257 1101 54-55 1.0855988641796017 1101 56-57 0.8678962206968066 1101 58-59 0.865377118685803 1101 60-61 0.85284136108951 1101 62-63 0.6975042813961316 1101 64-65 0.7061019843702532 1101 66-67 0.6674200671639774 1101 68-69 0.5477860988376122 1101 70-71 0.4245607706538337 1101 72-73 0.5180137388650259 1101 74-75 0.35285911733114617 1101 76-77 0.2231971071639407 1101 78-79 0.2290281230939044 1101 80-81 -0.041483678019030634 1101 82-83 0.033641960182510644 1101 84-85 -0.06089451335136431 1101 86-87 -0.1315573835399988 1101 88-89 -0.13961334686021232 1101 90-91 -0.0010236554570077772 1101 92-93 -0.14451626426710362 1101 94-95 -0.06359639325038913 1101 96-97 -0.6609655708012347 1101 98-99 -0.927835776397643 1101 100-101 -1.0516892652968899 1102 1 14.82234585385746 1102 2 8.076148828456503 1102 3 3.7939094622312624 1102 4 2.341571604367701 1102 5 1.7002234714462858 1102 6 1.355085634167061 1102 7 1.114414600770715 1102 8 0.9350951462165753 1102 9 1.0852725632830769 1102 10-11 1.0452777710978367 1102 12-13 0.7987255529721793 1102 14-15 0.8706932511530567 1102 16-17 1.0811905319802975 1102 18-19 0.9588941773770472 1102 20-21 0.9222787033371631 1102 22-23 0.9958396502197431 1102 24-25 0.8643023432922661 1102 26-27 0.9701541232379114 1102 28-29 1.139650242570859 1102 30-31 1.1254452698270612 1102 32-33 1.0231910786029275 1102 34-35 1.1197245771503361 1102 36-37 1.0975253017150308 1102 38-39 1.0680250489543184 1102 40-41 0.9939057530878443 1102 42-43 0.8846952603393206 1102 44-45 0.8483031895494122 1102 46-47 0.7420782395895529 1102 48-49 0.660298617413325 1102 50-51 0.6518017178212681 1102 52-53 0.8867374222426712 1102 54-55 0.8565033404409022 1102 56-57 0.7462698673045622 1102 58-59 0.6025422172527612 1102 60-61 0.8575116234087083 1102 62-63 0.5099888571066096 1102 64-65 0.5263408941677881 1102 66-67 0.45240718908326727 1102 68-69 0.1898580576976343 1102 70-71 0.24371527792385095 1102 72-73 0.2452524081630223 1102 74-75 0.09456866725926005 1102 76-77 -0.06616303012460989 1102 78-79 -0.033537774071287885 1102 80-81 0.07376824379357672 1102 82-83 0.04102718861922128 1102 84-85 -0.1665373430002397 1102 86-87 -0.20693002680464545 1102 88-89 -0.2516712251418234 1102 90-91 -0.049443695765429396 1102 92-93 0.24968009749311904 1102 94-95 0.06707312136563459 1102 96-97 -0.5581107888493015 1102 98-99 -0.8595810169233893 1102 100-101 -0.7893282003672546 1103 1 14.808114607138467 1103 2 7.860847621708519 1103 3 3.7324068261098553 1103 4 2.0867870343582666 1103 5 1.5726501549234868 1103 6 1.1047008007386907 1103 7 0.812259776456024 1103 8 0.8640847535335752 1103 9 0.8899377060354965 1103 10-11 0.9539351294121801 1103 12-13 0.5709566318802857 1103 14-15 0.6426980889643801 1103 16-17 0.8464718111850544 1103 18-19 0.8125739831580248 1103 20-21 0.6981848407444744 1103 22-23 0.8621461766833534 1103 24-25 0.9040636157404265 1103 26-27 0.7824221757428447 1103 28-29 0.7923239558229014 1103 30-31 0.7351149859782495 1103 32-33 0.7560600707384495 1103 34-35 0.6330038558726301 1103 36-37 0.7421065003816025 1103 38-39 0.7470990309245877 1103 40-41 0.6111092674011758 1103 42-43 0.6015672101417877 1103 44-45 0.510300426189815 1103 46-47 0.4061019583340837 1103 48-49 0.36144812201239773 1103 50-51 0.37439635019121553 1103 52-53 0.6196397557524627 1103 54-55 0.37944455198032756 1103 56-57 0.29675729169996146 1103 58-59 0.2054446280438853 1103 60-61 0.3022362341454041 1103 62-63 0.1529090039632166 1103 64-65 0.13262422519187922 1103 66-67 0.12717787053348673 1103 68-69 0.05088803567797129 1103 70-71 0.006396923552514977 1103 72-73 -0.02280785250691153 1103 74-75 0.03353419040142924 1103 76-77 -0.06613979052433194 1103 78-79 -0.1935523305620066 1103 80-81 -0.17747849728029053 1103 82-83 -0.34145216760520825 1103 84-85 -0.3667168564921788 1103 86-87 -0.4226341176919064 1103 88-89 -0.5814638958907885 1103 90-91 -0.5132750667969681 1103 92-93 -0.33483628421704026 1103 94-95 -0.45124520374919896 1103 96-97 -0.9618329337595242 1103 98-99 -1.1664550809222671 1103 100-101 -1.067522389583969 1104 1 14.917854865177684 1104 2 8.18225728122405 1104 3 3.9941452433717224 1104 4 2.530454045475693 1104 5 1.8274404288914852 1104 6 1.345622641424093 1104 7 1.1073906177578863 1104 8 1.055926450957827 1104 9 1.147302619777662 1104 10-11 1.0483524609896335 1104 12-13 0.88354542765574 1104 14-15 1.0155795871578448 1104 16-17 1.2055940439444868 1104 18-19 1.0324058464944201 1104 20-21 1.025889160107802 1104 22-23 1.1008205336422279 1104 24-25 1.2003286906735724 1104 26-27 1.113445261855908 1104 28-29 1.1502046315152157 1104 30-31 1.0341760569426484 1104 32-33 1.086283243073801 1104 34-35 1.2915889475327234 1104 36-37 1.4196203988333664 1104 38-39 1.2460172427680547 1104 40-41 1.2618170327155411 1104 42-43 1.0206050832056732 1104 44-45 0.9057093675070718 1104 46-47 0.873355987587324 1104 48-49 0.7066013341694131 1104 50-51 0.7651093365301058 1104 52-53 0.7073775065665338 1104 54-55 0.8800014309688393 1104 56-57 0.8267700566398304 1104 58-59 0.7015379480568171 1104 60-61 0.5091909424793322 1104 62-63 0.42210349974381955 1104 64-65 0.4275079151891923 1104 66-67 0.4731178984648423 1104 68-69 0.4503185986445928 1104 70-71 0.4850230135670195 1104 72-73 0.3490182673817337 1104 74-75 0.1866578958041316 1104 76-77 0.2059556724288747 1104 78-79 0.2176456122923085 1104 80-81 0.15230784327710012 1104 82-83 -0.0196358017194882 1104 84-85 -0.09168909808073877 1104 86-87 -0.1940931780646693 1104 88-89 -0.15452081644540883 1104 90-91 -0.2933931637415448 1104 92-93 0.039812615783233696 1104 94-95 0.2143373224664593 1104 96-97 -0.5063735763561219 1104 98-99 -0.9186964032410874 1104 100-101 -0.8212623106035437 1105 1 14.680570387049393 1105 2 7.949751109607252 1105 3 3.7840354774136813 1105 4 2.2370872169400045 1105 5 1.60631591865139 1105 6 1.2054100055790968 1105 7 0.9508145380066111 1105 8 1.0222252572982313 1105 9 1.001397528789873 1105 10-11 0.8244471205694879 1105 12-13 0.8106964542323354 1105 14-15 0.9094338568267162 1105 16-17 1.1481081649076472 1105 18-19 0.9644791010050326 1105 20-21 0.9979708670216283 1105 22-23 0.8947228819221138 1105 24-25 0.997137711950252 1105 26-27 0.9292941467203448 1105 28-29 1.1793504357328217 1105 30-31 0.9877351318001288 1105 32-33 0.6394716017093174 1105 34-35 0.8859891386943275 1105 36-37 0.9630699697415892 1105 38-39 0.9850565770980708 1105 40-41 1.1030952844672015 1105 42-43 0.9961986771958138 1105 44-45 0.8763871527783067 1105 46-47 0.7420609253523835 1105 48-49 0.7667890215384432 1105 50-51 0.7354227199909609 1105 52-53 0.8058918381847526 1105 54-55 0.885046672024778 1105 56-57 0.9729091738693683 1105 58-59 0.8989294934527159 1105 60-61 0.8555754047298905 1105 62-63 0.7399735989351015 1105 64-65 0.7753285523631028 1105 66-67 0.8115209706749908 1105 68-69 0.6672643308621886 1105 70-71 0.6171787746422588 1105 72-73 0.7292865172102516 1105 74-75 0.6447642640323181 1105 76-77 0.6990989288731129 1105 78-79 0.6777410987718753 1105 80-81 0.4868597008407427 1105 82-83 0.4641955317008346 1105 84-85 0.35441315451354427 1105 86-87 0.3234580826429756 1105 88-89 0.6250730489858363 1105 90-91 0.6564128449581936 1105 92-93 0.655232015856491 1105 94-95 0.7794184430046442 1105 96-97 0.35378219834357694 1105 98-99 0.0049810681436213144 1105 100-101 -0.025353446689331705 1106 1 14.655964481004695 1106 2 7.912380067053359 1106 3 3.522523291450856 1106 4 2.190233778624993 1106 5 1.6510004990548097 1106 6 1.1679030934599837 1106 7 0.9071807028740579 1106 8 0.9226772273553436 1106 9 1.0545842220320907 1106 10-11 0.8325085600234488 1106 12-13 0.6037637535126521 1106 14-15 0.7870311629572484 1106 16-17 1.0395959418050822 1106 18-19 0.9003427857628807 1106 20-21 0.7049961591876226 1106 22-23 0.644893673163736 1106 24-25 0.8278771436296282 1106 26-27 0.7698731862865529 1106 28-29 0.9216544100981423 1106 30-31 0.7885343068851327 1106 32-33 0.6609629790986418 1106 34-35 0.7182786904588738 1106 36-37 0.8121270844786395 1106 38-39 0.8794875239964952 1106 40-41 1.1094382369943503 1106 42-43 0.6842650067161387 1106 44-45 0.8076963700375615 1106 46-47 0.8142706988824742 1106 48-49 0.8319302247836333 1106 50-51 0.7246414132496817 1106 52-53 0.6538168612939295 1106 54-55 0.6430420336833578 1106 56-57 0.8075218682340264 1106 58-59 0.7174361249604289 1106 60-61 0.7669998480463889 1106 62-63 0.59164571276429 1106 64-65 0.6935139250810707 1106 66-67 0.7482178237006423 1106 68-69 0.6615274783317346 1106 70-71 0.6288488561020991 1106 72-73 0.6348475749501716 1106 74-75 0.3734785479780456 1106 76-77 0.34183062929567853 1106 78-79 0.36733397495621745 1106 80-81 0.4053127892168291 1106 82-83 0.4167732878825383 1106 84-85 0.3827976168951075 1106 86-87 0.3028389734191137 1106 88-89 0.41491104748373786 1106 90-91 0.8949533252163562 1106 92-93 1.1639499518770897 1106 94-95 0.9446110744793401 1106 96-97 0.5983106886542267 1106 98-99 0.32714663506119024 1106 100-101 0.38290148051676987 1107 1 14.797587923249203 1107 2 7.994054767954726 1107 3 3.724772727497868 1107 4 2.17598588357054 1107 5 1.3819759162123972 1107 6 1.135944111161642 1107 7 0.8760321784911724 1107 8 0.8486271169594488 1107 9 0.7703909801285889 1107 10-11 0.7588981024671924 1107 12-13 0.5838767027592766 1107 14-15 0.7560197354258094 1107 16-17 0.9498917956131407 1107 18-19 0.7520367700248798 1107 20-21 0.758917278807921 1107 22-23 0.8095921169648008 1107 24-25 0.9215683236922771 1107 26-27 0.8400637060093388 1107 28-29 0.9633814682272046 1107 30-31 0.9122783590370176 1107 32-33 1.0013649572445686 1107 34-35 0.799886713797676 1107 36-37 0.837652617420197 1107 38-39 0.8902733493794059 1107 40-41 0.9137284404963708 1107 42-43 0.7278545465257125 1107 44-45 0.7497247568686021 1107 46-47 0.68654103471588 1107 48-49 0.7402656385167816 1107 50-51 0.7697243508580378 1107 52-53 0.8249285491297833 1107 54-55 0.7959624959571769 1107 56-57 0.604934180129959 1107 58-59 0.7992486710584963 1107 60-61 0.8012106760634765 1107 62-63 0.6770456763647772 1107 64-65 0.49068676649413945 1107 66-67 0.5232758653589258 1107 68-69 0.5948936191212191 1107 70-71 0.5394272102454174 1107 72-73 0.6120308273424833 1107 74-75 0.6114066912676002 1107 76-77 0.6184150707786742 1107 78-79 0.5758936827303884 1107 80-81 0.48299521783068045 1107 82-83 0.527886469415531 1107 84-85 0.5188345121124023 1107 86-87 0.7231645245604312 1107 88-89 0.7757608114855792 1107 90-91 1.0553978752273743 1107 92-93 1.2935525745578182 1107 94-95 1.3799540441049025 1107 96-97 1.007393923804667 1107 98-99 0.7583263355075616 1107 100-101 0.6297189177994511 1108 1 14.281593210757464 1108 2 7.497915138569887 1108 3 3.247102658221717 1108 4 -0.16561897310371876 1108 5 0.17168730638643837 1108 6 0.40063285807114113 1108 7 0.5302732007427764 1108 8 0.47829346539645456 1108 9 0.6338802459972328 1108 10-11 0.6365877551072785 1108 12-13 0.20271957074292146 1108 14-15 0.28252704246847316 1108 16-17 0.5201544574818797 1108 18-19 0.31819794111004285 1108 20-21 0.2380977150273509 1108 22-23 -0.45797660840299415 1108 24-25 0.253028997849583 1108 26-27 0.3746050881052483 1108 28-29 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1.646292093855056 2311 100-101 1.7893865577782648 2312 1 -14.19095580885038 2312 2 -7.291415565778891 2312 3 -3.3321222781836326 2312 4 -1.4438787313831938 2312 5 -1.9800939523616279 2312 6 -0.7230730364530231 2312 7 -0.2505522517225103 2312 8 0.06489172977980218 2312 9 0.028904649302937457 2312 10-11 0.05787035877568769 2312 12-13 -0.6173818155595825 2312 14-15 0.074772146593304 2312 16-17 -0.2490584294109297 2312 18-19 0.004798031177109863 2312 20-21 0.051147702247568816 2312 22-23 0.2712617163496205 2312 24-25 0.24466736641861075 2312 26-27 0.2546509736227094 2312 28-29 -0.47392386910102147 2312 30-31 0.03188525856246116 2312 32-33 0.3402373602778468 2312 34-35 -0.618972243451104 2312 36-37 0.008170519445670266 2312 38-39 0.4198679559678098 2312 40-41 0.4055204536839625 2312 42-43 0.3116971225367209 2312 44-45 0.4227236057989501 2312 46-47 0.3328001814254975 2312 48-49 0.4014239370561441 2312 50-51 0.45034464297017607 2312 52-53 0.43042865723487367 2312 54-55 0.2557212814275758 2312 56-57 0.30665472634718327 2312 58-59 0.2764120344684322 2312 60-61 0.36840547815731384 2312 62-63 0.22141478229532296 2312 64-65 0.24876459641672355 2312 66-67 0.12775482137963934 2312 68-69 0.4462761266643014 2312 70-71 0.5919001638009824 2312 72-73 0.352603222266179 2312 74-75 0.4903849894267829 2312 76-77 0.4527900011189203 2312 78-79 0.6699243944229281 2312 80-81 0.7718097665196098 2312 82-83 0.618383354881157 2312 84-85 0.6392233786417947 2312 86-87 0.6960892247482917 2312 88-89 1.144762192577705 2312 90-91 1.3127887147259791 2312 92-93 1.9410339036938566 2312 94-95 2.282743839257723 2312 96-97 1.8979867157865336 2312 98-99 1.7504499803818554 2312 100-101 1.8396031421006285 2313 1 -14.19095580885038 2313 2 -7.371134425700266 2313 3 -3.4542388414629492 2313 4 -0.9215576735107831 2313 5 -0.29668375914503287 2313 6 -0.7448752007810526 2313 7 -0.2863410600931431 2313 8 0.02263372525770535 2313 9 -2.3356981244591424 2313 10-11 -0.40387021810393975 2313 12-13 -0.6659626600075583 2313 14-15 -0.6820495721686086 2313 16-17 -0.6537172494611312 2313 18-19 0.05291447483610767 2313 20-21 -1.061089188352419 2313 22-23 -1.8070618355128474 2313 24-25 -1.049828329291799 2313 26-27 -0.14442172218026883 2313 28-29 0.35896520576938684 2313 30-31 -1.0023725628707751 2313 32-33 -0.035752910489456724 2313 34-35 -0.9687982069476817 2313 36-37 -1.1220074621460796 2313 38-39 -0.5276146403818487 2313 40-41 -0.0962581541193046 2313 42-43 0.14664016165512095 2313 44-45 0.05619763338614803 2313 46-47 -0.41789493088487717 2313 48-49 0.159758755008653 2313 50-51 -0.787075846952451 2313 52-53 0.06732664628630047 2313 54-55 -0.6372619446728649 2313 56-57 -0.1834678342831353 2313 58-59 0.014393063842959464 2313 60-61 -1.5714151858018184 2313 62-63 -0.35230213239207586 2313 64-65 -0.12725296326751945 2313 66-67 -1.35191000095611 2313 68-69 -0.37347681228611407 2313 70-71 -0.08624964933268231 2313 72-73 -0.9866886450155015 2313 74-75 -0.44526072547733264 2313 76-77 0.13825720013854692 2313 78-79 0.23314639152753713 2313 80-81 0.08365649154329802 2313 82-83 0.2733310801212845 2313 84-85 0.5641954048432396 2313 86-87 0.4035552682322354 2313 88-89 0.7893985570184441 2313 90-91 1.1956660445146028 2313 92-93 1.8323120085132487 2313 94-95 1.8098901434152666 2313 96-97 1.5303788856412588 2313 98-99 1.4822062865676742 2313 100-101 1.4881735771986904 2314 1 -14.19095580885038 2314 2 -7.237523029396364 2314 3 -3.47999982776469 2314 4 -1.296385347643497 2314 5 -1.2187906096259589 2314 6 -6.034841546155526 2314 7 -1.9080526840259182 2314 8 -4.151112036252073 2314 9 -1.4509577868659278 2314 10-11 -0.3525259328045749 2314 12-13 -2.8264233298578816 2314 14-15 -2.912415315035787 2314 16-17 -2.552556730575361 2314 18-19 -1.288525674642436 2314 20-21 -1.9154916878796016 2314 22-23 -0.94286183787694 2314 24-25 -2.8021849973994435 2314 26-27 -4.503365949728092 2314 28-29 -1.766330433145022 2314 30-31 -1.8741113739320525 2314 32-33 -3.94110142921312 2314 34-35 -2.5535187030030144 2314 36-37 -0.7252226778193673 2314 38-39 -1.1330733429647921 2314 40-41 -2.2164116685200383 2314 42-43 -1.1077072037263846 2314 44-45 -2.178276236574945 2314 46-47 -1.7317298442237963 2314 48-49 -0.7991523329493084 2314 50-51 -2.011472875281104 2314 52-53 -0.6934154356736038 2314 54-55 -0.29330107556284446 2314 56-57 -0.914037311749901 2314 58-59 -1.275860139510005 2314 60-61 -0.38098940328533715 2314 62-63 -2.6882340598750574 2314 64-65 -0.8804274826801404 2314 66-67 -1.8721578682018674 2314 68-69 -1.6261276417468302 2314 70-71 -2.538382445790589 2314 72-73 -2.57846373653987 2314 74-75 -1.9443125532629146 2314 76-77 -0.7936784305352873 2314 78-79 -0.35139152016326847 2314 80-81 -0.036109479628212426 2314 82-83 -0.1336956527626434 2314 84-85 0.14518062585264246 2314 86-87 0.27431171208828076 2314 88-89 0.41899824765931726 2314 90-91 0.9470184775093067 2314 92-93 1.4357582725627438 2314 94-95 1.4777956115742406 2314 96-97 1.5561131914069577 2314 98-99 1.4125068056560757 2314 100-101 1.4292098458412923 2315 1 -14.19095580885038 2315 2 -7.408122130618299 2315 3 -4.649228460746816 2315 4 -2.896577221612592 2315 5 -3.3283287656161313 2315 6 -2.7079960215317023 2315 7 -3.1116747259688964 2315 8 -3.5348208422573926 2315 9 -2.536894603104521 2315 10-11 -4.461699906952077 2315 12-13 -4.2016558087349125 2315 14-15 -3.374983270399838 2315 16-17 -4.453736797562939 2315 18-19 -1.5945587644865853 2315 20-21 -3.359417151575027 2315 22-23 -4.233992716121129 2315 24-25 -4.864091702889731 2315 26-27 -4.490389420627203 2315 28-29 -2.4953734617241423 2315 30-31 -3.2084479780994215 2315 32-33 -4.327001427530014 2315 34-35 -3.8556295270512138 2315 36-37 -5.2296416699761 2315 38-39 -3.6407752314085897 2315 40-41 -4.478886632333282 2315 42-43 -3.366431788841002 2315 44-45 -2.0807287309365456 2315 46-47 -1.3927247275846213 2315 48-49 -1.0434636484641686 2315 50-51 -0.8026843321767849 2315 52-53 -0.7857137576179234 2315 54-55 -2.1925409432707923 2315 56-57 -4.31294097923568 2315 58-59 -3.670700160380509 2315 60-61 -4.568512966755225 2315 62-63 -2.5483682454205514 2315 64-65 -3.1948540067317097 2315 66-67 -1.9294617023150415 2315 68-69 -1.2981883092662727 2315 70-71 -2.033182282529406 2315 72-73 -2.3228947628972954 2315 74-75 -2.8495680486007124 2315 76-77 -2.732146636681989 2315 78-79 -2.0676116029720326 2315 80-81 -1.5912808197164097 2315 82-83 -1.2298630123075327 2315 84-85 -1.0540638703940353 2315 86-87 -1.5873825013967533 2315 88-89 -2.2499659274509263 2315 90-91 -1.6611515245104194 2315 92-93 -0.47023126693973083 2315 94-95 -0.22951579651139298 2315 96-97 -1.2713555028401906 2315 98-99 -0.7341524413227525 2315 100-101 -0.2912476641731807 2316 1 -14.19095580885038 2316 2 -7.353860207738611 2316 3 -5.1488401297018385 2316 4 -2.925135888963009 2316 5 -2.0853178973095687 2316 6 -1.1639934409292323 2316 7 -4.7310077394839745 2316 8 -2.824520971287516 2316 9 -1.1658234049440281 2316 10-11 -4.417215427787134 2316 12-13 -3.596886139912229 2316 14-15 -1.8107137587292357 2316 16-17 -3.1240903690505917 2316 18-19 -2.2410488969905984 2316 20-21 -3.1898162558082035 2316 22-23 -2.9778989376890124 2316 24-25 -3.373555359404925 2316 26-27 -4.054371716205441 2316 28-29 -3.4920024463563237 2316 30-31 -2.5222006455490913 2316 32-33 -2.695820284951729 2316 34-35 -3.9956593652672936 2316 36-37 -2.2571701641283894 2316 38-39 -3.209960191128033 2316 40-41 -2.827554251562077 2316 42-43 -6.557383448036052 2316 44-45 -3.3826111439652777 2316 46-47 -4.738655729553159 2316 48-49 -4.224587926805306 2316 50-51 -3.2465286234209323 2316 52-53 -4.661636302323554 2316 54-55 -4.767210200189616 2316 56-57 -4.720601212317021 2316 58-59 -3.3596066714390496 2316 60-61 -3.2709949356225714 2316 62-63 -2.168378563694855 2316 64-65 -2.2433764670714886 2316 66-67 -2.5436943039262623 2316 68-69 -2.8705426747854546 2316 70-71 -2.9315302213558887 2316 72-73 -3.7267985444724587 2316 74-75 -4.2626774264439256 2316 76-77 -3.823075240013612 2316 78-79 -4.01073744042716 2316 80-81 -4.035303664665211 2316 82-83 -3.5409654961374137 2316 84-85 -3.0933484769348674 2316 86-87 -3.2739377279438777 2316 88-89 -2.9827442313664925 2316 90-91 -2.2487719733036577 2316 92-93 -1.9628685889029711 2316 94-95 -1.25233096913054 2316 96-97 -1.5815056657820747 2316 98-99 -1.914218838073836 2316 100-101 -1.9077886436441567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 28018.0 3 8468.0 4 9521.0 5 11276.0 6 13359.0 7 15490.0 8 19520.0 9 23513.0 10 27973.0 11 32733.0 12 37168.0 13 40097.0 14 43968.0 15 47759.0 16 52948.0 17 56870.0 18 55712.0 19 59208.0 20 63752.0 21 69914.0 22 77952.0 23 87524.0 24 97164.0 25 108618.0 26 122438.0 27 137446.0 28 155757.0 29 177446.0 30 201456.0 31 230280.0 32 265738.0 33 306577.0 34 358855.0 35 420874.0 36 451125.0 37 275951.0 38 36382.0 39 210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.624660244275724 18.79980832803576 13.509894383496418 24.0656370441921 2 15.295152016453017 20.518109086433952 40.56946559646327 23.617273300649767 3 19.057156364960395 24.878214037896225 29.820201614153756 26.24442798298962 4 12.514769186081912 16.724108642452666 38.43975795000972 32.321364221455696 5 13.590866078127267 37.50625902157317 35.08300261630061 13.819872283998947 6 31.45066126229854 36.84659098309469 18.237966333369407 13.464781421237356 7 27.80179753460669 32.41198830063115 22.133477009792895 17.652737154969273 8 25.451375921265367 35.40330351829174 20.601839298117813 18.54348126232508 9 26.23779139512096 16.663043689318087 20.460965852609704 36.638199062951244 10-11 24.492478134400706 26.34757214040721 28.74340326639457 20.416546458797516 12-13 25.659077538304086 23.847875975842754 28.358955032943367 22.13409145290979 14-15 22.737958449451444 25.45325137020948 26.22849959930964 25.58029058102944 16-17 22.43538968181406 28.29120777765227 26.920052257048415 22.353350283485252 18-19 22.266454368153994 27.58924713637969 28.42175348514643 21.72254501031989 20-21 23.1732829780881 26.8523893375145 28.216782599724954 21.75754508467245 22-23 22.754747737043306 26.74563075512445 28.065318626434742 22.434302881397493 24-25 22.700430860570552 26.856876818383636 28.22516514386449 22.217527177181324 26-27 22.509070141638762 26.95920983722325 28.263639573543387 22.268080447594603 28-29 22.4908411454137 27.259654119944265 28.147968227186986 22.101536507455048 30-31 22.68352729334682 27.03338648199137 28.03025859162574 22.25282763303607 32-33 22.388340723114332 26.99405890756843 28.205845540128234 22.411754829189 34-35 22.711974969643858 27.029057424735186 28.106855992164565 22.152111613456388 36-37 22.752656888660923 26.784043801422335 28.176174684469483 22.28712462544726 38-39 22.512747781731395 27.087047623582738 28.171309433234104 22.228895161451764 40-41 22.693285088687208 27.005653773116784 28.16526421015561 22.1357969280404 42-43 22.484330087475758 27.050858322812104 27.84914526619218 22.615666323519953 44-45 22.691422564153836 26.966031108869192 27.931522483667592 22.411023843309373 46-47 22.826902916359217 27.073458276538364 27.57306835979368 22.526570447308742 48-49 22.7107935173199 27.1081366252655 27.69169586141615 22.489373995998452 50-51 22.58005297775591 27.23353173622619 27.719329059037452 22.467086226980445 52-53 22.65127109160433 27.345018436186518 27.435350059936574 22.568360412272582 54-55 22.654094149744882 27.14656864424268 27.4743267783682 22.72501042764424 56-57 22.67940107092807 27.32184320215693 27.376686019655722 22.62206970725928 58-59 22.68324835083976 27.154326052520826 27.538698749764134 22.623726846875275 60-61 22.634663405308526 27.229904070059813 27.700023443775752 22.435409080855912 62-63 22.541865088461854 27.133877653823017 27.74685128497475 22.577405972740376 64-65 22.50086975762991 27.26089897816953 27.596565603416344 22.64166566078422 66-67 22.503540684253892 27.329308217526847 27.359209303455152 22.807941794764112 68-69 22.418861620204297 27.173035984271483 27.585201280607386 22.822901114916842 70-71 22.627659400546897 26.87730997901457 27.56604771845924 22.928982901979296 72-73 22.488999197097797 26.963081665676075 27.53551681023165 23.012402326994483 74-75 22.378999136872242 27.359288959100166 27.21356947775437 23.04814242627322 76-77 22.460357910756997 27.03068957915024 27.409425915697295 23.099526594395464 78-79 22.489656772069 27.04924629285934 27.432040841216416 23.029056093855246 80-81 22.51652771877655 27.106798174080595 27.271316774638915 23.105357332503946 82-83 22.535115117758338 27.0405902956954 27.455758993089685 22.968535593456572 84-85 22.538330524314063 27.034866396329416 27.232506820140816 23.194296259215704 86-87 22.33222842607654 26.911587709424516 27.513055723656983 23.24312814084196 88-89 22.446179380025704 26.804916679563345 27.390338938904822 23.358565001506125 90-91 22.352384298164495 26.830775938828694 27.47939698373382 23.33744277927299 92-93 22.368588642691936 26.838213993443897 27.500968296394312 23.29222906746985 94-95 22.38546444500469 26.708753978280964 27.465028879021737 23.440752697692606 96-97 22.263118201433723 26.632684431756733 27.47116661550717 23.63303075130237 98-99 22.310048676642328 26.705650987241235 27.20148913906569 23.78281119705075 100-101 22.411373487278357 26.583003161186102 27.278620627667205 23.72700272386834 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4357.0 1 2735.0 2 998.5 3 785.5 4 617.5 5 553.0 6 651.5 7 1141.0 8 1536.0 9 1483.0 10 1409.5 11 1432.0 12 1572.0 13 1752.0 14 1912.0 15 2140.5 16 2431.0 17 2693.0 18 3067.5 19 3583.0 20 4206.5 21 5018.5 22 6285.0 23 8224.0 24 10792.5 25 14520.5 26 19923.5 27 26445.5 28 33300.5 29 41195.5 30 49683.5 31 58988.5 32 68781.0 33 78742.0 34 89149.5 35 99315.5 36 110380.0 37 118832.0 38 124314.5 39 130013.0 40 134936.5 41 139968.5 42 144993.5 43 149617.0 44 155233.5 45 159745.0 46 163644.0 47 170702.5 48 173910.5 49 168948.5 50 161756.5 51 155268.5 52 150251.0 53 144499.5 54 136435.5 55 126075.5 56 114905.0 57 103921.0 58 92135.5 59 79137.5 60 65329.0 61 52583.0 62 41160.0 63 31129.5 64 23025.0 65 16518.0 66 11978.5 67 8857.5 68 6251.0 69 4420.0 70 3063.0 71 1985.0 72 1254.0 73 840.0 74 610.5 75 390.5 76 228.0 77 153.0 78 107.5 79 72.0 80 49.5 81 37.0 82 24.0 83 18.5 84 16.5 85 11.5 86 9.5 87 12.0 88 7.5 89 7.0 90 6.5 91 4.0 92 6.5 93 6.0 94 3.0 95 3.0 96 5.5 97 4.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.17004251535802 2 0.08737166178772587 3 0.11943552467924314 4 0.1957645434209966 5 0.12194199183742958 6 0.1490402122457473 7 0.15629950863785333 8 0.15301272623230694 9 0.1422065423522012 10-11 0.12942592443710896 12-13 0.14139075822996128 14-15 0.1501042784921472 16-17 0.14397998609620105 18-19 0.15108558403049377 20-21 0.17938974618473136 22-23 0.19779809224744976 24-25 0.22419875811646087 26-27 0.21683305509971484 28-29 0.2151778409386483 30-31 0.23084326067731364 32-33 0.22183416645779439 34-35 0.20677171759208904 36-37 0.19371917163625013 38-39 0.18991217906579713 40-41 0.19090530756243704 42-43 0.18523028758163754 44-45 0.20011539207294293 46-47 0.22627959877608736 48-49 0.2292235153911271 50-51 0.2392848528987529 52-53 0.26644218809853726 54-55 0.25314136001853843 56-57 0.23005112247166037 58-59 0.1867199803265974 60-61 0.19681678670910321 62-63 0.20261003627283602 64-65 0.19050332698046374 66-67 0.20045825786344956 68-69 0.187701285864944 70-71 0.20129768790227615 72-73 0.22151494658387444 74-75 0.2525620350621651 76-77 0.2527748483114451 78-79 0.24777373695336552 80-81 0.2528221401446184 82-83 0.24338741942653924 84-85 0.21334528240318182 86-87 0.22583032636094075 88-89 0.22763923897982058 90-91 0.21535518531304831 92-93 0.2132743446534218 94-95 0.25269208760339174 96-97 0.28035781000978943 98-99 0.2994518876535211 100-101 0.29430890079592154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4229060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.03125548425471 #Duplication Level Percentage of deduplicated Percentage of total 1 79.37717576512348 53.20751748330584 2 11.96391261431266 16.039121660825796 3 3.9897253252195597 8.023088927603807 4 1.7832016427447257 4.781209795790577 5 0.9459446629182211 3.170392918701924 6 0.5392175280185558 2.168665672932006 7 0.3419726262834527 1.6045998136719375 8 0.22753152959391312 1.2201379272746282 9 0.16350372627223603 0.9863874043543713 >10 0.644236117378636 7.021979094018212 >50 0.016951441845760147 0.7711437055307977 >100 0.006249343719593865 0.6748484254712814 >500 1.8883830871703385E-4 0.08470779291077093 >1k 1.888382605299956E-4 0.24619937760802713 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.364591658666465E-5 2 0.0 0.0 0.0 0.0 2.364591658666465E-5 3 0.0 0.0 0.0 0.0 2.364591658666465E-5 4 0.0 0.0 0.0 0.0 2.364591658666465E-5 5 0.0 2.364591658666465E-5 0.0 2.364591658666465E-5 2.364591658666465E-5 6 0.0 2.364591658666465E-5 0.0 2.364591658666465E-5 7.093774975999395E-5 7 0.0 2.364591658666465E-5 0.0 2.364591658666465E-5 7.093774975999395E-5 8 0.0 2.364591658666465E-5 0.0 2.364591658666465E-5 7.093774975999395E-5 9 0.0 2.364591658666465E-5 0.0 2.364591658666465E-5 7.093774975999395E-5 10-11 0.0 4.72918331733293E-5 0.0 3.546887487999697E-5 7.093774975999395E-5 12-13 0.0 7.093774975999395E-5 0.0 5.911479146666162E-5 2.009902909866495E-4 14-15 0.0 9.45836663466586E-5 0.0 1.0640662463999092E-4 2.1281324927998184E-4 16-17 0.0 9.45836663466586E-5 0.0 1.418754995199879E-4 2.1281324927998184E-4 18-19 0.0 9.45836663466586E-5 0.0 1.536984578133202E-4 2.364591658666465E-4 20-21 0.0 9.45836663466586E-5 0.0 2.364591658666465E-4 2.6010508245331116E-4 22-23 0.0 9.45836663466586E-5 0.0 3.6651170709330207E-4 3.0739691562664046E-4 24-25 3.546887487999697E-5 1.3005254122665558E-4 0.0 6.502627061332779E-4 3.0739691562664046E-4 26-27 4.72918331733293E-5 1.6552141610665254E-4 0.0 9.576596217599183E-4 3.0739691562664046E-4 28-29 4.72918331733293E-5 1.6552141610665254E-4 0.0 0.0026956344908797697 3.0739691562664046E-4 30-31 4.72918331733293E-5 1.7734437439998488E-4 0.0 0.007814975431892666 3.0739691562664046E-4 32-33 5.911479146666162E-5 2.009902909866495E-4 0.0 0.02054830151381158 3.0739691562664046E-4 34-35 7.093774975999395E-5 2.1281324927998184E-4 0.0 0.03918128378410332 3.310428322133051E-4 36-37 7.093774975999395E-5 2.1281324927998184E-4 0.0 0.06806477089471419 3.428657905066374E-4 38-39 9.45836663466586E-5 2.1281324927998184E-4 0.0 0.10995351212799062 3.6651170709330207E-4 40-41 9.45836663466586E-5 2.2463620757331418E-4 0.0 0.1562995086378533 3.783346653866344E-4 42-43 9.45836663466586E-5 2.719280407466435E-4 0.0 0.2057312972622758 3.783346653866344E-4 44-45 9.45836663466586E-5 2.837509990399758E-4 0.0 0.2676954216776305 4.256264985599637E-4 46-47 1.3005254122665558E-4 2.837509990399758E-4 1.1822958293332324E-5 0.3420500063843975 4.847412900266253E-4 48-49 1.418754995199879E-4 2.9557395733330815E-4 2.364591658666465E-5 0.42083820045116405 5.202101649066223E-4 50-51 1.418754995199879E-4 3.0739691562664046E-4 2.364591658666465E-5 0.5034333870883837 5.202101649066223E-4 52-53 1.418754995199879E-4 3.1921987391997277E-4 2.364591658666465E-5 0.5878493093027766 5.43856081493287E-4 54-55 1.418754995199879E-4 3.310428322133051E-4 2.364591658666465E-5 0.6797728100334354 5.675019980799516E-4 56-57 1.418754995199879E-4 3.310428322133051E-4 2.364591658666465E-5 0.7939234723555589 5.675019980799516E-4 58-59 1.418754995199879E-4 3.6651170709330207E-4 2.364591658666465E-5 0.9208783985093614 5.675019980799516E-4 60-61 1.418754995199879E-4 3.783346653866344E-4 2.364591658666465E-5 1.0551635588050299 5.911479146666162E-4 62-63 1.418754995199879E-4 3.783346653866344E-4 2.364591658666465E-5 1.1937168070445914 6.620856644266102E-4 64-65 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 1.3431944687471922 6.857315810132748E-4 66-67 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 1.5181978974050971 6.857315810132748E-4 68-69 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 1.7161142192354801 6.857315810132748E-4 70-71 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 1.9317531555475687 7.093774975999395E-4 72-73 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 2.1532208102982695 7.803152473599335E-4 74-75 1.418754995199879E-4 4.0198058197329906E-4 2.364591658666465E-5 2.385128137221983 8.276070805332627E-4 76-77 1.418754995199879E-4 4.0198058197329906E-4 4.72918331733293E-5 2.642324771935135 8.512529971199274E-4 78-79 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 2.9294571370470033 8.512529971199274E-4 80-81 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 3.235825455302124 8.630759554132596E-4 82-83 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 3.55289355081271 9.340137051732536E-4 84-85 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 3.8830851300288955 9.45836663466586E-4 86-87 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 4.237478304871532 9.931284966399152E-4 88-89 1.418754995199879E-4 4.0198058197329906E-4 7.093774975999395E-5 4.625495973100405 0.0010640662463999094 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10130 0.0 29.466946 1 GTATCAA 14160 0.0 28.77206 1 TACATGG 11535 0.0 25.066166 2 ACATGGG 11745 0.0 24.014605 3 TATCAAC 17550 0.0 22.747501 2 ATCAACG 18240 0.0 21.783379 3 TCAACGC 18525 0.0 21.496454 4 CAACGCA 18520 0.0 21.484173 5 AACGCAG 19020 0.0 21.018658 6 GGTATCA 10785 0.0 20.664503 1 ACGCAGA 19880 0.0 19.49257 7 CATGGGG 6470 0.0 18.764038 4 AGAGTAC 13505 0.0 18.732893 10-11 CGCAGAG 20790 0.0 18.411673 8 CATGGGA 6670 0.0 18.343044 4 GAGTACT 7600 0.0 18.289583 12-13 GTATAGA 1630 0.0 17.941687 1 AGTACTT 8555 0.0 17.434084 12-13 GAGTACA 9360 0.0 17.238401 1 GTACTTT 8355 0.0 16.554655 14-15 >>END_MODULE