FastQCFastQC Report
Fri 27 May 2016
SRR939670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229060
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76590.18110407513726454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61510.14544603292457425No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49840.11785124826793661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120600.024.5348151
TACATGG118550.024.3550532
GTATCAA154750.024.2437081
ACATGGG116950.023.5153273
GGTATCA114350.023.1107671
TCAACGC175550.021.0398964
ATCAACG175350.021.0369953
CAACGCA176900.020.7993355
AACGCAG180250.020.3997426
TATCAAC182500.020.318842
CATGGGA64900.017.9529134
CATGGGG62400.017.8406284
ACGCAGA200950.017.829167
CGCAGAG198000.017.7614738
AGAGTAC142750.017.56895810-11
GAGTACT84950.017.56389412-13
AGTACTT94100.016.05642712-13
GCAGAGT213000.015.49276359
GTACTTT94200.015.48888814-15
GTATAGG13500.015.0313251
Produced by FastQC (version 0.11.5)