##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939670_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4229060 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.522603604583523 31.0 31.0 34.0 30.0 34.0 2 31.73463464694282 31.0 31.0 34.0 30.0 34.0 3 31.79573687769859 31.0 31.0 34.0 30.0 34.0 4 35.58096740173939 37.0 35.0 37.0 33.0 37.0 5 35.33375478238663 37.0 35.0 37.0 33.0 37.0 6 35.33093831726199 37.0 35.0 37.0 32.0 37.0 7 35.24135316122259 37.0 35.0 37.0 32.0 37.0 8 35.2045991307761 37.0 35.0 37.0 32.0 37.0 9 36.82217017493249 39.0 37.0 39.0 32.0 39.0 10-11 36.697566244035315 39.0 35.0 39.0 32.0 39.0 12-13 36.63645822002998 39.0 35.0 39.0 32.0 39.0 14-15 37.74732150879865 39.5 37.0 41.0 32.0 41.0 16-17 37.69556851877249 39.0 37.0 41.0 32.0 41.0 18-19 37.646602791164 39.0 36.5 41.0 32.0 41.0 20-21 37.57972599111859 39.0 36.0 41.0 32.0 41.0 22-23 37.44352657564565 39.0 36.0 41.0 32.0 41.0 24-25 37.322686719980325 39.0 36.0 41.0 31.0 41.0 26-27 37.049857651582144 39.0 36.0 40.0 31.0 41.0 28-29 36.86755106335687 39.0 36.0 40.0 30.5 41.0 30-31 36.65403068294137 38.5 35.5 40.0 30.0 41.0 32-33 36.51611800258213 38.0 35.0 40.0 30.0 41.0 34-35 36.51063345045944 38.0 35.0 40.0 30.0 41.0 36-37 36.46365114233423 38.0 35.0 40.0 30.0 41.0 38-39 36.53785214681277 39.0 35.0 40.0 30.0 41.0 40-41 36.44934157945265 39.0 35.0 40.0 30.0 41.0 42-43 36.299876331856254 38.5 35.0 40.0 30.0 41.0 44-45 36.13008978354528 38.0 35.0 40.0 29.5 41.0 46-47 35.90863643457411 38.0 35.0 40.0 29.0 41.0 48-49 35.66892843799804 38.0 34.5 40.0 28.0 41.0 50-51 35.45943590301391 38.0 34.0 40.0 27.0 41.0 52-53 35.25559332333899 38.0 34.0 40.0 27.0 41.0 54-55 34.98166885785494 38.0 34.0 40.0 26.5 41.0 56-57 34.1792375847115 37.5 32.5 40.0 24.0 41.0 58-59 31.094285609568082 35.5 28.0 39.0 10.0 40.5 60-61 30.842781492814005 35.0 27.0 39.0 11.0 40.0 62-63 32.669236189602415 36.0 31.0 39.0 21.5 40.0 64-65 32.734114791466666 36.0 31.0 39.0 21.5 40.0 66-67 27.796379100793082 32.0 19.0 37.0 5.0 39.5 68-69 20.21967765886509 21.0 8.0 32.5 2.0 37.5 70-71 18.233354338789233 16.5 7.5 29.0 2.0 35.5 72-73 20.005558682071193 20.5 8.5 31.0 2.0 36.0 74-75 21.468254647604905 24.5 9.5 32.5 2.0 36.0 76-77 23.313756248433457 27.5 12.5 33.5 2.0 36.0 78-79 25.518613592618692 31.0 18.0 35.0 2.0 37.0 80-81 27.14795320946026 32.5 24.0 35.0 2.0 37.0 82-83 28.311957621788295 33.5 28.0 35.0 2.0 36.5 84-85 28.36940159278894 34.0 29.0 35.0 2.0 36.0 86-87 28.09417577428554 34.0 28.5 35.0 2.0 36.0 88-89 27.662856875996084 33.5 27.0 35.0 2.0 35.5 90-91 25.814780944228744 32.5 19.5 35.0 2.0 35.0 92-93 24.17722366199581 31.5 6.5 34.0 2.0 35.0 94-95 20.39067357757989 29.0 2.0 34.0 2.0 35.0 96-97 17.634996074777845 19.5 2.0 33.0 2.0 35.0 98-99 17.925487933488768 24.0 2.0 33.5 2.0 35.0 100-101 17.142417936846485 21.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3378002707680601 1101 2 0.2778186417678832 1101 3 0.2750482895511617 1101 4 0.21996147956932788 1101 5 0.3500796977303864 1101 6 0.3915709947937813 1101 7 0.29232923852312354 1101 8 0.4519175611146409 1101 9 0.43707431446522094 1101 10-11 0.3932101214321406 1101 12-13 0.320262294408721 1101 14-15 0.4157105002245416 1101 16-17 0.3933526864065655 1101 18-19 0.5082760856875979 1101 20-21 0.5076466634911867 1101 22-23 0.4140701599293948 1101 24-25 0.4546522662499086 1101 26-27 0.49615235249552114 1101 28-29 0.41415709129321243 1101 30-31 0.24825178226075906 1101 32-33 0.3266060184938908 1101 34-35 0.18805542211116943 1101 36-37 0.11194982010182031 1101 38-39 0.1345258632859796 1101 40-41 0.28819588389603723 1101 42-43 0.3025986782788479 1101 44-45 0.35252477462206855 1101 46-47 0.3347377337044364 1101 48-49 0.21737272621326298 1101 50-51 0.11771575722911365 1101 52-53 0.12106376913766326 1101 54-55 0.2482961365180003 1101 56-57 -3.3047040810925594 1101 58-59 -10.413725308101544 1101 60-61 -12.037829335004712 1101 62-63 -4.0878081645738575 1101 64-65 -1.2331437339118025 1101 66-67 -2.2539641974805313 1101 68-69 -4.9710511742192764 1101 70-71 -4.8105115984327504 1101 72-73 -3.862103547528342 1101 74-75 -6.416499231745078 1101 76-77 -5.1511895416146345 1101 78-79 1.3086920566604725 1101 80-81 1.9490727924844933 1101 82-83 0.46533367505851686 1101 84-85 0.1942091326706894 1101 86-87 0.2253761267042833 1101 88-89 0.06855008470391866 1101 90-91 1.4628609549311662 1101 92-93 1.0220402946693277 1101 94-95 -8.9090991973402 1101 96-97 -9.76539159309221 1101 98-99 -8.77749202304311 1101 100-101 -9.83571653975363 1102 1 0.24884164542383402 1102 2 0.29780031022907494 1102 3 0.28025705538089696 1102 4 0.18541060518171548 1102 5 0.25002460994222986 1102 6 0.27330106579646696 1102 7 0.28689127022597205 1102 8 0.3036865720792079 1102 9 0.25696746977842366 1102 10-11 0.2671034887110366 1102 12-13 0.26746533716464427 1102 14-15 0.4160956197667858 1102 16-17 0.37118870298880324 1102 18-19 0.3233379371613694 1102 20-21 0.3281388548263351 1102 22-23 0.25188201591617343 1102 24-25 0.30067025188865415 1102 26-27 0.14528780614578807 1102 28-29 0.25960518619328354 1102 30-31 0.22409270084089172 1102 32-33 0.04663866936368066 1102 34-35 0.10461963869119728 1102 36-37 -0.07777648210419841 1102 38-39 0.061383052562618445 1102 40-41 0.12471731135688913 1102 42-43 0.2530086921439718 1102 44-45 -0.03605210865529784 1102 46-47 -0.05035065424403484 1102 48-49 0.16562983245059115 1102 50-51 0.1544227129067437 1102 52-53 0.10575012105079651 1102 54-55 -0.11766610330720795 1102 56-57 -3.720446319159155 1102 58-59 -10.2462263460929 1102 60-61 -8.269608117354956 1102 62-63 -2.381313366759432 1102 64-65 -0.6286926872147092 1102 66-67 -1.8544703820455872 1102 68-69 -5.126534447272439 1102 70-71 -4.8401243708800035 1102 72-73 -3.7437970777413128 1102 74-75 -4.306022739847233 1102 76-77 -4.2094492450140635 1102 78-79 1.918814670663366 1102 80-81 2.321368031583546 1102 82-83 0.844601566506455 1102 84-85 0.49283403433082995 1102 86-87 0.49389694971350906 1102 88-89 0.31632892878043606 1102 90-91 1.6309727831411216 1102 92-93 1.301416760304388 1102 94-95 -8.881014004599908 1102 96-97 -9.786832243570675 1102 98-99 -8.75454539705367 1102 100-101 -9.842730711756804 1103 1 0.1664702346586573 1103 2 0.10722846701964883 1103 3 0.19688176012481406 1103 4 0.19492150819750975 1103 5 0.24005890629295124 1103 6 0.2425727389317629 1103 7 0.31346268949209843 1103 8 0.26019484697773265 1103 9 0.28754973492736013 1103 10-11 0.3346665544172893 1103 12-13 0.2935098909560381 1103 14-15 0.38670390112383046 1103 16-17 0.26806813804019924 1103 18-19 0.28033716931614094 1103 20-21 0.2880758263807337 1103 22-23 0.264137648207182 1103 24-25 0.32781780347807654 1103 26-27 0.41893691467802796 1103 28-29 0.3534305458412703 1103 30-31 0.30494845910047275 1103 32-33 0.34737546405423103 1103 34-35 0.33981927016964164 1103 36-37 0.2750563478884658 1103 38-39 0.4147500345555102 1103 40-41 0.39448896710388226 1103 42-43 0.26373117322798123 1103 44-45 0.18133754326406404 1103 46-47 0.1433254696335169 1103 48-49 0.1422465164963569 1103 50-51 0.04301397360261916 1103 52-53 0.1569026341016837 1103 54-55 0.0864704075400553 1103 56-57 0.508583364048107 1103 58-59 -8.537745437617733 1103 60-61 -7.851518103372555 1103 62-63 -2.3660698174045365 1103 64-65 -0.479818759010179 1103 66-67 -1.9429160778342514 1103 68-69 -3.7097306430437165 1103 70-71 -4.178176318286745 1103 72-73 -3.0879760822804876 1103 74-75 -3.991089033429077 1103 76-77 -0.8572453746545996 1103 78-79 2.9761343167369496 1103 80-81 2.5731674721771114 1103 82-83 0.9134504555597225 1103 84-85 0.5498002563170417 1103 86-87 0.48041544230802913 1103 88-89 0.23809336645111046 1103 90-91 1.6980646535314996 1103 92-93 1.1852384932499191 1103 94-95 -7.642828443291801 1103 96-97 -9.201494030105287 1103 98-99 -8.66265187747969 1103 100-101 -10.030025578123583 1104 1 0.18703693340741268 1104 2 0.17774421453526656 1104 3 0.18194019940628792 1104 4 0.09784504135879502 1104 5 0.21855588741701837 1104 6 0.06229486773047199 1104 7 0.2313175188141514 1104 8 0.2549473591346896 1104 9 0.28702795324178254 1104 10-11 0.16602072597898854 1104 12-13 0.19832990808395579 1104 14-15 0.32162002926207833 1104 16-17 0.3169980552217808 1104 18-19 0.3239144671296117 1104 20-21 0.3649579787259043 1104 22-23 0.24485784165560887 1104 24-25 0.3219252523339904 1104 26-27 0.2524588859906203 1104 28-29 0.3691136139346938 1104 30-31 0.4056970180127024 1104 32-33 0.3572833818828087 1104 34-35 0.2337280089490008 1104 36-37 0.25788903543676867 1104 38-39 0.24389484025358144 1104 40-41 0.2517047988369896 1104 42-43 0.23510738471978243 1104 44-45 0.25592305427412043 1104 46-47 0.09396270353622072 1104 48-49 0.206977784761051 1104 50-51 0.3188577643551085 1104 52-53 0.2992526703831544 1104 54-55 0.25680972762323506 1104 56-57 0.822730207421273 1104 58-59 -8.038966144801922 1104 60-61 -7.401665172565995 1104 62-63 -1.9420430752377733 1104 64-65 -0.08967104507420487 1104 66-67 -1.4094142453701188 1104 68-69 -3.2827132792077514 1104 70-71 -3.736035384946547 1104 72-73 -2.7331836752090837 1104 74-75 -3.3890205700050373 1104 76-77 -0.019042176934785715 1104 78-79 4.037362777939141 1104 80-81 3.2087864712533545 1104 82-83 1.534811048350761 1104 84-85 1.2412706387108443 1104 86-87 1.1842890458269757 1104 88-89 1.1284210041845029 1104 90-91 2.5219614902353733 1104 92-93 2.4114373494894927 1104 94-95 -6.57047229264851 1104 96-97 -8.061984763642126 1104 98-99 -5.94244351337273 1104 100-101 -5.232408388282565 1105 1 0.21260348809152774 1105 2 0.20630322389055067 1105 3 0.20602064029370482 1105 4 0.16754393562933956 1105 5 0.21492324049428646 1105 6 0.2225887472577881 1105 7 0.18166832236887842 1105 8 0.2715556764461837 1105 9 0.22413426540008885 1105 10-11 0.21994302360734252 1105 12-13 0.15944992749356146 1105 14-15 0.26309132908239263 1105 16-17 0.3264744149556478 1105 18-19 0.37483372493210254 1105 20-21 0.3169663022208127 1105 22-23 0.3269254367765839 1105 24-25 0.3710902303540635 1105 26-27 0.31191082650624935 1105 28-29 0.3602909923062896 1105 30-31 0.3923992602314357 1105 32-33 0.3014863852370482 1105 34-35 0.33048737956199403 1105 36-37 0.2596831405536406 1105 38-39 0.4040591302386076 1105 40-41 0.32467429629890887 1105 42-43 0.30763673363320976 1105 44-45 0.35088051190860625 1105 46-47 0.32622588821101317 1105 48-49 0.25651009908983724 1105 50-51 0.23999102568843256 1105 52-53 0.3646564023313985 1105 54-55 0.33943922677249105 1105 56-57 0.8614508342720413 1105 58-59 -8.281088562639898 1105 60-61 -7.606804238965829 1105 62-63 -1.9965820891310493 1105 64-65 -0.16932189741496018 1105 66-67 -1.7530552136916846 1105 68-69 -3.4440272375158685 1105 70-71 -3.747308967701759 1105 72-73 -2.890282017060322 1105 74-75 -3.5852380802613695 1105 76-77 -0.12078736501254994 1105 78-79 3.8150912980663954 1105 80-81 3.2514296053394887 1105 82-83 1.616060940272142 1105 84-85 1.170029560764803 1105 86-87 1.0872090550706446 1105 88-89 1.055561367003783 1105 90-91 2.527440408222958 1105 92-93 2.276470732028077 1105 94-95 -6.507560788209819 1105 96-97 -7.865066353700046 1105 98-99 -5.821236051153086 1105 100-101 -5.185223096197799 1106 1 0.06612020922981898 1106 2 0.10704890742691475 1106 3 0.1379048014306754 1106 4 0.04640151708917983 1106 5 0.14314108985548302 1106 6 0.09300570278913511 1106 7 0.14158968927653603 1106 8 0.09601591215976413 1106 9 0.025525767826550805 1106 10-11 0.05297041829553706 1106 12-13 0.11817405347157006 1106 14-15 0.055558305659594964 1106 16-17 0.11979488349415846 1106 18-19 0.07797977078730867 1106 20-21 0.05381847159455333 1106 22-23 0.19268285028224597 1106 24-25 0.08514917112855613 1106 26-27 0.07920721817373533 1106 28-29 0.1296147497755058 1106 30-31 0.18026137274009812 1106 32-33 0.07562541374386456 1106 34-35 0.09805793475990043 1106 36-37 0.15563640431185632 1106 38-39 0.18106235520642144 1106 40-41 0.11553869290186469 1106 42-43 0.12868341416537987 1106 44-45 0.012806393358545165 1106 46-47 0.03327609495558903 1106 48-49 -0.026076261132672585 1106 50-51 0.00940943792308957 1106 52-53 0.08486810919152532 1106 54-55 -0.03222221969675587 1106 56-57 0.6197256209248394 1106 58-59 -8.623122603279032 1106 60-61 -7.84580948185976 1106 62-63 -2.146318293779295 1106 64-65 -0.501327072219361 1106 66-67 0.6292438809238767 1106 68-69 -2.377016821880858 1106 70-71 -2.6134993687284656 1106 72-73 -1.24156050118102 1106 74-75 -1.1901783368557375 1106 76-77 2.2930121133803745 1106 78-79 4.481749711456509 1106 80-81 3.476267995868028 1106 82-83 1.7827514382748504 1106 84-85 1.3072877913448444 1106 86-87 1.0425317994501633 1106 88-89 0.9878912566574733 1106 90-91 2.3544159847143256 1106 92-93 2.1984078895170995 1106 94-95 -6.601264002448371 1106 96-97 -7.91893172418963 1106 98-99 -5.725855564787498 1106 100-101 -5.086862609584614 1107 1 0.10291604627494522 1107 2 0.1855874430170168 1107 3 0.16094859698993247 1107 4 0.08968221481038086 1107 5 0.14091925278162165 1107 6 0.1330691145729972 1107 7 0.15463678952846038 1107 8 0.17824169450982907 1107 9 0.2609755412874719 1107 10-11 0.13437010231422875 1107 12-13 0.15398985756556272 1107 14-15 0.30585397687141125 1107 16-17 0.30670705829324163 1107 18-19 0.2607273293857304 1107 20-21 0.24112307864744054 1107 22-23 0.2736862106517961 1107 24-25 0.34352450159781966 1107 26-27 0.2806581698294366 1107 28-29 0.2749047096163011 1107 30-31 0.24551977336464148 1107 32-33 0.27000787025602335 1107 34-35 0.3025554747993695 1107 36-37 0.2876451815037271 1107 38-39 0.33978870450286536 1107 40-41 0.28786187687745723 1107 42-43 0.3110437789268161 1107 44-45 0.16647984063192922 1107 46-47 0.16152698159505263 1107 48-49 0.23509481238956198 1107 50-51 0.27484286153678994 1107 52-53 0.3218757129780343 1107 54-55 0.3049493764697999 1107 56-57 0.8410691610888321 1107 58-59 -8.398158793255757 1107 60-61 -7.622570383154816 1107 62-63 -1.9645013773449165 1107 64-65 -0.1519561013102333 1107 66-67 0.9114734648678642 1107 68-69 -2.4635225404359886 1107 70-71 -2.4784885296957366 1107 72-73 -1.0752145004578182 1107 74-75 -0.967489421585185 1107 76-77 2.539968443543117 1107 78-79 4.6373121318325765 1107 80-81 3.5598808361555854 1107 82-83 1.8872675821774507 1107 84-85 1.4231200380808637 1107 86-87 1.2049457696791919 1107 88-89 1.125723806323652 1107 90-91 2.462966682411576 1107 92-93 2.225764024631136 1107 94-95 -6.946201252201718 1107 96-97 -8.56834776045025 1107 98-99 -6.425475091090936 1107 100-101 -5.774016574736869 1108 1 -0.04117589761871798 1108 2 -0.05310194035180871 1108 3 -0.08006875876635178 1108 4 -0.061121927071091875 1108 5 -0.08579735146307144 1108 6 -0.0203787814393408 1108 7 -0.11685228050654217 1108 8 -0.04792412762427034 1108 9 -0.08018134593682902 1108 10-11 -0.08020114441166726 1108 12-13 -0.09719371199209803 1108 14-15 -0.17324323005131248 1108 16-17 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98-99 -1.0620308997806163 2312 100-101 -0.430396983769338 2313 1 -0.26524361132700847 2313 2 -0.2746522033663723 2313 3 -0.259558370766122 2313 4 -0.17784583702981394 2313 5 -0.24093177913765373 2313 6 -0.17704399686761718 2313 7 -0.2376443162535935 2313 8 -0.3397410243390908 2313 9 -0.356348159518987 2313 10-11 -0.27647007321857586 2313 12-13 -0.3154599210707616 2313 14-15 -0.3753180530360609 2313 16-17 -0.3962710966736509 2313 18-19 -0.3640164391669245 2313 20-21 -0.3583549647136053 2313 22-23 -0.39215219929823064 2313 24-25 -0.3364174407135536 2313 26-27 -0.38989735371794865 2313 28-29 -0.372469477436546 2313 30-31 -0.4191647571226014 2313 32-33 -0.4697731965726817 2313 34-35 -0.4238584816207194 2313 36-37 -0.5320438612297167 2313 38-39 -0.5660057225967847 2313 40-41 -0.508719387761758 2313 42-43 -0.5587301775584947 2313 44-45 -0.44906520990232934 2313 46-47 -0.5968100749528773 2313 48-49 -0.6487059927306831 2313 50-51 -0.5745466309174887 2313 52-53 -0.48469502248276797 2313 54-55 -0.584134226275701 2313 56-57 0.2388677357227067 2313 58-59 3.4005189990283675 2313 60-61 3.3721941294102606 2313 62-63 0.6048109730017828 2313 64-65 -0.15485074212782735 2313 66-67 -1.961174931051712 2313 68-69 -4.6809236678000055 2313 70-71 -3.6819377030152705 2313 72-73 -2.8795872937058107 2313 74-75 -3.5294693718481085 2313 76-77 0.6055493583269786 2313 78-79 3.1407707354839687 2313 80-81 2.3709586542395016 2313 82-83 0.7686251749262567 2313 84-85 0.4956220781488092 2313 86-87 0.4730997699879218 2313 88-89 0.5991627693386334 2313 90-91 2.2619501904732893 2313 92-93 3.7292407461151136 2313 94-95 6.068167729054299 2313 96-97 1.538960628975893 2313 98-99 3.12389385121174 2313 100-101 4.428719186277323 2314 1 -0.3742206829524264 2314 2 -0.33768184577964533 2314 3 -0.414258788873763 2314 4 -0.21286328069673033 2314 5 -0.27044391371562426 2314 6 -0.3296351328505409 2314 7 -0.33256226516205345 2314 8 -0.5299616791267283 2314 9 -0.476058912112741 2314 10-11 -0.4165637843798251 2314 12-13 -0.47791892929782165 2314 14-15 -0.7377426551921147 2314 16-17 -0.6502865173480288 2314 18-19 -0.47746756650400357 2314 20-21 -0.5328140928342151 2314 22-23 -0.5353974643474047 2314 24-25 -0.49898198903571966 2314 26-27 -0.563971218275384 2314 28-29 -0.5066085319999374 2314 30-31 -0.5707379799962595 2314 32-33 -0.5104389350494003 2314 34-35 -0.4729879447002645 2314 36-37 -0.44769946785729786 2314 38-39 -0.4361304456595718 2314 40-41 -0.5776835919387793 2314 42-43 -0.5671837703122051 2314 44-45 -0.5787984298715259 2314 46-47 -0.5080247654586643 2314 48-49 -0.5846023320935956 2314 50-51 -0.5384182299934608 2314 52-53 -0.6231626562907238 2314 54-55 -0.4492460903333111 2314 56-57 0.2445648744885105 2314 58-59 3.4626034665659198 2314 60-61 3.457003743521902 2314 62-63 0.7464534639506653 2314 64-65 -0.12369505275680837 2314 66-67 -2.0579379741188255 2314 68-69 -4.948658118788874 2314 70-71 -3.618833760375045 2314 72-73 -2.831768174231044 2314 74-75 -4.078870706212641 2314 76-77 0.6754571331702728 2314 78-79 3.4641396766381867 2314 80-81 2.4734675111642836 2314 82-83 0.9294594144972095 2314 84-85 0.5191017941868523 2314 86-87 0.6296629707741523 2314 88-89 0.6489117105603981 2314 90-91 2.2809674179940913 2314 92-93 3.973617738712111 2314 94-95 6.548182150052892 2314 96-97 3.7533166148723573 2314 98-99 6.278069819478787 2314 100-101 6.586841003188056 2315 1 -0.4686416106798994 2315 2 -0.4135616889142604 2315 3 -0.4540343468230681 2315 4 -0.3310753182636361 2315 5 -0.45075368918941905 2315 6 -0.5178193150298256 2315 7 -0.5584778742777488 2315 8 -0.6982107450034576 2315 9 -0.6733516377329636 2315 10-11 -0.5524639930848423 2315 12-13 -0.5912388118170924 2315 14-15 -0.7303769669704891 2315 16-17 -0.8119432817469914 2315 18-19 -0.734580368200568 2315 20-21 -0.8140535734280192 2315 22-23 -0.8356177407132392 2315 24-25 -0.5547586157566897 2315 26-27 -0.6236690588516822 2315 28-29 -0.6992493658101395 2315 30-31 -0.7082899458628944 2315 32-33 -0.8095161727374816 2315 34-35 -0.6137207686138169 2315 36-37 -0.611718890888902 2315 38-39 -0.8334277998185229 2315 40-41 -0.715197898331212 2315 42-43 -0.6271626894221711 2315 44-45 -0.5952678226072408 2315 46-47 -0.6759374207250985 2315 48-49 -0.6976487100516522 2315 50-51 -0.5985860776387995 2315 52-53 -0.7048588895017502 2315 54-55 -0.6004086951065872 2315 56-57 0.12298389189181336 2315 58-59 3.1931631831310447 2315 60-61 3.2758951243412326 2315 62-63 0.5536765090242213 2315 64-65 -0.3366932518344683 2315 66-67 -2.3493503516729355 2315 68-69 -5.196834474529945 2315 70-71 -3.969333221781447 2315 72-73 -3.2390795823166805 2315 74-75 -0.6891466933537203 2315 76-77 1.6966768793580407 2315 78-79 3.514572234621159 2315 80-81 2.461071022121299 2315 82-83 0.6677441621997708 2315 84-85 0.3245680984249084 2315 86-87 0.3510848999490932 2315 88-89 0.4320329025353544 2315 90-91 2.149034287755427 2315 92-93 3.815267223681982 2315 94-95 6.472051734662582 2315 96-97 4.083585575050098 2315 98-99 6.381625799550822 2315 100-101 6.749308393028837 2316 1 -0.46909052798482165 2316 2 -0.4629080603555913 2316 3 -0.530684650374706 2316 4 -0.30548393019726916 2316 5 -0.5388090977020994 2316 6 -0.571992322920515 2316 7 -0.45253793945450127 2316 8 -0.3834372094126479 2316 9 -0.5410845950041434 2316 10-11 -0.5805679533170931 2316 12-13 -0.5710589347265582 2316 14-15 -0.7052948756899511 2316 16-17 -0.6934797741127099 2316 18-19 -0.7073947137035432 2316 20-21 -0.7050771917304459 2316 22-23 -0.8445573157409498 2316 24-25 -0.740226081141877 2316 26-27 -0.7629149977112561 2316 28-29 -0.6474242305766538 2316 30-31 -0.7378733067667653 2316 32-33 -0.619061300196087 2316 34-35 -0.505399152759658 2316 36-37 -0.41258860354874827 2316 38-39 -0.6096207081244245 2316 40-41 -0.614533930820329 2316 42-43 -0.5973402919432189 2316 44-45 -0.5413413987267504 2316 46-47 -0.5499050763470024 2316 48-49 -0.6449280147777898 2316 50-51 -0.5968970030163732 2316 52-53 -0.5395622029291545 2316 54-55 -0.46281316318818 2316 56-57 0.378837496464115 2316 58-59 3.467420226025286 2316 60-61 3.368732194861167 2316 62-63 0.6781612156461136 2316 64-65 0.2014481767605858 2316 66-67 -2.031355818910864 2316 68-69 -3.320980063519375 2316 70-71 -3.2995537723099755 2316 72-73 -0.6279896086189751 2316 74-75 5.7885868222208785 2316 76-77 5.923629700651322 2316 78-79 4.909435843163614 2316 80-81 3.2335166805884867 2316 82-83 1.5688744164552837 2316 84-85 1.2885755176570157 2316 86-87 1.1551587572880955 2316 88-89 1.090408591109572 2316 90-91 2.6146533428579097 2316 92-93 4.197204143666767 2316 94-95 6.910363859358615 2316 96-97 3.9227023803965757 2316 98-99 6.338413321676182 2316 100-101 6.425720383732049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 22.0 4 61.0 5 134.0 6 305.0 7 870.0 8 1912.0 9 3118.0 10 5005.0 11 7678.0 12 11290.0 13 15552.0 14 19335.0 15 25623.0 16 34477.0 17 45125.0 18 51951.0 19 54900.0 20 59182.0 21 61019.0 22 62765.0 23 63800.0 24 67588.0 25 72563.0 26 89836.0 27 128254.0 28 181858.0 29 243613.0 30 298284.0 31 332690.0 32 349286.0 33 350396.0 34 360051.0 35 386352.0 36 395365.0 37 311461.0 38 128681.0 39 8653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.70051018948037 19.766879996556323 14.19940270153738 24.33320711242593 2 15.466299757051663 21.202202876642644 39.46861643369435 23.86288093261134 3 18.802909303808676 25.090227055560664 30.22466258994626 25.882201050684394 4 12.796507971038482 17.455604791608536 37.876171064018955 31.871716173334026 5 13.359968104526294 37.804891033657064 35.15990918781299 13.675231674003651 6 31.515490439955922 37.425148851044916 17.682842050006382 13.376518658992778 7 27.6010744704497 32.58873130199146 21.967222030427568 17.842972197131278 8 25.34991260460963 35.860035903993705 20.325528912362476 18.46452257903419 9 25.80081625704057 16.517689510198483 20.39178919192445 37.289705040836495 10-11 24.332570358424803 26.576603311374157 28.656900114919154 20.433926215281883 12-13 25.37050786699645 23.897733775354336 28.495031992925142 22.236726364724078 14-15 22.338091743548798 25.596089985938885 26.24723090130298 25.81858736920934 16-17 22.128669758449814 28.499720623429468 26.94046122317045 22.43114839495027 18-19 22.004590873702497 27.75896671646123 28.39178135476293 21.84466105507334 20-21 22.849593053775543 26.997275990409214 28.21781908982138 21.93531186599386 22-23 22.420005864187313 26.933278317167407 28.08643055430758 22.5602852643377 24-25 22.4878672687238 27.023595809561286 28.144117741642578 22.344419180072336 26-27 22.393354450733213 27.11142864979797 28.18464688100607 22.310570018462748 28-29 22.341532749723015 27.384402605757053 28.08804071770283 22.186023926817096 30-31 22.512656856162142 27.14567098807012 27.966641872601066 22.375030283166673 32-33 22.31017135103569 27.110679576294505 28.070128559093188 22.509020513576615 34-35 22.59973996121291 27.163554276446273 28.00940745940308 22.22729830293774 36-37 22.695274900852887 26.931697184120274 28.04794088543571 22.325087029591124 38-39 22.432930302607907 27.202254750664228 28.052330018669686 22.312484928058176 40-41 22.614494433624106 27.131661217579566 28.05392152628241 22.19992282251391 42-43 22.399587237008557 27.110576223289627 27.841739474129533 22.648097065572276 44-45 22.549148623848 27.036287449923574 27.927911722534354 22.486652203694067 46-47 22.687526322385153 27.12828946239557 27.600213809656626 22.58397040556265 48-49 22.55980708384706 27.1870141303036 27.701654412199723 22.551524373649624 50-51 22.453687900659748 27.260841750335814 27.76324116840343 22.52222918060101 52-53 22.628206553912506 27.339910685681645 27.431879731839366 22.60000302856648 54-55 22.58506921094472 27.121660064304866 27.502743443091166 22.79052728165925 56-57 22.48216251159598 27.26555653661857 27.599194205873133 22.65308674591232 58-59 21.43134619182834 26.965397639221557 29.177253866721465 22.426002302228643 60-61 21.765602156500492 27.21254518657347 28.626725541423376 22.395127115502664 62-63 22.49843252812434 27.15390971763988 27.740344603799983 22.6073131504358 64-65 22.40885465930158 27.27064100866407 27.624865215088345 22.69563911694601 66-67 22.047917514220984 27.022633106463655 28.21226473047414 22.717184648841215 68-69 21.166824620457014 26.663011337572062 29.688946018535667 22.48121802343526 70-71 22.01237912382815 26.53337940156878 28.74799485181418 22.706246622788893 72-73 21.376237567752764 26.72829796302098 29.00655281871827 22.88891165050799 74-75 21.58185384925697 26.980523364311402 28.502665005147048 22.934957781284577 76-77 20.626384066982382 27.153064215467392 28.920227360675003 23.300324356875223 78-79 21.99985256121385 26.99884783722758 27.93844906287194 23.06285053868663 80-81 22.105855652071682 27.14163908204139 27.5131281999969 23.239377065890025 82-83 22.366974761391813 27.05163669485144 27.445554345277724 23.13583419847902 84-85 22.325758687310955 27.04561139002532 27.270262687489723 23.358367235174 86-87 22.128359705022362 26.93631837419663 27.52740394682098 23.40791797396003 88-89 22.275105032267827 26.88389788977804 27.318900251415357 23.522096826538775 90-91 21.986975861832533 26.87497790598829 27.604809839658568 23.53323639252061 92-93 21.879907711948093 26.942667278008763 27.595755241714347 23.58166976832879 94-95 20.706789956233173 26.997904147382286 28.392358599165625 23.902947297218923 96-97 20.18817247268527 27.10103754688358 28.462468559504007 24.248321420927148 98-99 21.154544067787334 26.798364049448807 27.951387442846798 24.09570443991706 100-101 20.389537249749175 26.836302168617575 28.40178423772748 24.37237634390577 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 209.0 2 176.0 3 225.0 4 251.5 5 258.5 6 334.5 7 506.5 8 737.5 9 953.0 10 1119.0 11 1274.5 12 1464.0 13 1681.0 14 1875.5 15 2113.5 16 2385.0 17 2688.5 18 3079.5 19 3608.5 20 4256.0 21 5084.5 22 6403.0 23 8155.5 24 10632.5 25 14182.5 26 19100.0 27 25225.0 28 32072.0 29 39771.5 30 48267.5 31 57626.5 32 68051.5 33 78686.0 34 88990.0 35 99625.0 36 110403.5 37 119586.0 38 126671.0 39 132728.0 40 138684.0 41 145013.0 42 150694.0 43 155780.5 44 160806.5 45 165287.0 46 169358.5 47 172840.0 48 174734.0 49 171975.0 50 165405.5 51 159032.5 52 152973.5 53 145702.5 54 136698.0 55 125917.5 56 113922.5 57 101722.5 58 88834.0 59 74940.5 60 60905.5 61 47969.5 62 36952.5 63 27577.5 64 19945.5 65 14195.0 66 10003.0 67 7121.5 68 4900.0 69 3215.5 70 2070.5 71 1287.0 72 784.0 73 518.5 74 329.0 75 173.5 76 99.5 77 54.0 78 25.5 79 12.5 80 6.5 81 3.0 82 3.0 83 1.5 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024189772668157937 2 0.013927444869545478 3 1.6552141610665254E-4 4 0.0 5 0.06514450019626111 6 0.0 7 0.0 8 9.45836663466586E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 5.0838720661329E-4 16-17 2.364591658666465E-5 18-19 4.1380354026663137E-4 20-21 0.0 22-23 0.0 24-25 9.45836663466586E-5 26-27 9.45836663466586E-5 28-29 9.340137051732536E-4 30-31 0.005332154190292878 32-33 0.0053558001068795425 34-35 0.001785266702293181 36-37 0.08921604328148572 38-39 0.08259518663721963 40-41 0.08830567549289912 42-43 0.09162792677332551 44-45 0.09500929284521856 46-47 0.09000818148713899 48-49 0.08015965722879316 50-51 0.07423635512383367 52-53 0.062377927955621346 54-55 0.05115794053524897 56-57 0.06901060755818078 58-59 0.6908509219542878 60-61 0.328619125763172 62-63 0.0027547492823464313 64-65 0.0023645916586664648 66-67 1.074801492530255 68-69 2.319782646734735 70-71 2.305654211574203 72-73 1.770925453883369 74-75 1.2618525156890656 76-77 0.7344539921400973 78-79 0.5657285543359517 80-81 0.03817633232917008 82-83 8.276070805332627E-5 84-85 0.001454223870079876 86-87 0.001454223870079876 88-89 5.911479146666162E-5 90-91 0.3338094044539448 92-93 0.0542437326498087 94-95 0.9306323391013606 96-97 1.6226182650518082 98-99 0.6378958917584522 100-101 0.554603150581926 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4229060.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.2688127300525 #Duplication Level Percentage of deduplicated Percentage of total 1 67.83580861717404 31.386823252997075 2 15.30156453682584 14.159704480624146 3 6.278478508845688 8.714932390663211 4 3.231336317598333 5.980403797870989 5 1.9415425690331878 4.49164347670108 6 1.252187199427512 3.476232901996827 7 0.8554756173021303 2.7707288792454827 8 0.604979739841918 2.239335543057727 9 0.43361907599463717 1.8056735841036842 >10 2.113218516175943 16.6274956484225 >50 0.10546403870993593 3.3562666219230013 >100 0.04381946183405942 3.554967264571518 >500 0.001789858161693173 0.5306760099019101 >1k 6.136654929482894E-4 0.5783534364265989 >5k 1.0227758215804677E-4 0.32676271149414016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7659 0.18110407513726454 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6151 0.14544603292457425 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4984 0.11785124826793661 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1822958293332324E-4 2 0.0 0.0 0.0 0.0 1.1822958293332324E-4 3 0.0 0.0 0.0 0.0 1.1822958293332324E-4 4 0.0 0.0 0.0 0.0 1.418754995199879E-4 5 0.0 0.0 0.0 0.0 1.6552141610665254E-4 6 0.0 0.0 0.0 0.0 2.837509990399758E-4 7 0.0 0.0 0.0 0.0 2.837509990399758E-4 8 0.0 0.0 0.0 0.0 2.837509990399758E-4 9 0.0 0.0 0.0 0.0 2.837509990399758E-4 10-11 0.0 9.45836663466586E-5 0.0 1.1822958293332324E-5 2.837509990399758E-4 12-13 0.0 1.418754995199879E-4 0.0 2.364591658666465E-5 4.8474129002662534E-4 14-15 2.364591658666465E-5 1.418754995199879E-4 0.0 5.911479146666162E-5 5.793249563732839E-4 16-17 2.364591658666465E-5 1.418754995199879E-4 0.0 9.45836663466586E-5 6.620856644266102E-4 18-19 2.364591658666465E-5 1.418754995199879E-4 0.0 1.0640662463999092E-4 7.093774975999395E-4 20-21 2.364591658666465E-5 1.891673326933172E-4 0.0 1.7734437439998488E-4 8.630759554132596E-4 22-23 2.364591658666465E-5 1.891673326933172E-4 0.0 2.9557395733330815E-4 8.74898913706592E-4 24-25 2.364591658666465E-5 1.891673326933172E-4 0.0 5.556790397866192E-4 8.74898913706592E-4 26-27 2.364591658666465E-5 1.891673326933172E-4 0.0 9.103677885865891E-4 8.985448302932567E-4 28-29 2.364591658666465E-5 1.891673326933172E-4 0.0 0.002766572240639764 8.985448302932567E-4 30-31 2.364591658666465E-5 1.891673326933172E-4 0.0 0.008193310097279301 9.103677885865891E-4 32-33 2.364591658666465E-5 1.891673326933172E-4 0.0 0.02168330550997148 9.45836663466586E-4 34-35 2.364591658666465E-5 1.891673326933172E-4 0.0 0.041392176984956466 9.45836663466586E-4 36-37 4.72918331733293E-5 1.891673326933172E-4 0.0 0.07222645221396717 9.45836663466586E-4 38-39 9.45836663466586E-5 1.891673326933172E-4 0.0 0.11666895243860337 9.45836663466586E-4 40-41 9.45836663466586E-5 1.891673326933172E-4 0.0 0.16578152118910586 9.45836663466586E-4 42-43 9.45836663466586E-5 1.891673326933172E-4 0.0 0.21782618359635475 9.576596217599183E-4 44-45 9.45836663466586E-5 1.891673326933172E-4 0.0 0.2828761001262692 9.813055383465828E-4 46-47 9.45836663466586E-5 1.891673326933172E-4 0.0 0.3599972570736759 9.931284966399152E-4 48-49 9.45836663466586E-5 2.009902909866495E-4 0.0 0.4362789839822561 0.0010404203298132447 50-51 9.45836663466586E-5 2.1281324927998184E-4 0.0 0.5131755047220895 0.0010640662463999092 52-53 9.45836663466586E-5 2.364591658666465E-4 0.0 0.5902257239197364 0.0011586499127465679 54-55 9.45836663466586E-5 2.364591658666465E-4 0.0 0.6745115935929025 0.0011586499127465679 56-57 1.418754995199879E-4 2.482821241599788E-4 0.0 0.7602280412195618 0.0011704728710399 58-59 1.418754995199879E-4 2.6010508245331116E-4 0.0 0.8254671250821695 0.0011822958293332324 60-61 1.418754995199879E-4 2.837509990399758E-4 0.0 0.8791433557338983 0.0011822958293332324 62-63 1.7734437439998488E-4 3.073969156266404E-4 0.0 0.9279958194019475 0.0011822958293332324 64-65 1.891673326933172E-4 3.5468874879996976E-4 0.0 0.983693775921836 0.0011822958293332324 66-67 1.891673326933172E-4 3.5468874879996976E-4 0.0 1.0342014537509518 0.0011822958293332324 68-69 1.891673326933172E-4 3.5468874879996976E-4 0.0 1.0945576558384134 0.0011941187876265648 70-71 2.009902909866495E-4 3.783346653866344E-4 0.0 1.1854998510307255 0.0012059417459198971 72-73 2.2463620757331418E-4 3.783346653866344E-4 2.364591658666465E-5 1.3038949553801555 0.0012532335790932264 74-75 2.364591658666465E-4 3.783346653866344E-4 2.364591658666465E-5 1.467501052243288 0.0013005254122665556 76-77 2.482821241599788E-4 3.783346653866344E-4 2.364591658666465E-5 1.6829271753060964 0.0013005254122665556 78-79 2.6010508245331116E-4 3.783346653866344E-4 2.364591658666465E-5 1.9482816512416472 0.0013241713288532203 80-81 2.6010508245331116E-4 3.783346653866344E-4 2.364591658666465E-5 2.2532193915432743 0.0013359942871465527 82-83 2.6010508245331116E-4 3.783346653866344E-4 2.364591658666465E-5 2.5613256846675148 0.001418754995199879 84-85 2.719280407466435E-4 3.783346653866344E-4 2.364591658666465E-5 2.842381049216611 0.001418754995199879 86-87 2.837509990399758E-4 3.783346653866344E-4 2.364591658666465E-5 3.1088468832317346 0.0014424009117865438 88-89 2.837509990399758E-4 3.783346653866344E-4 2.364591658666465E-5 3.3744969331246186 0.0014660468283732083 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12060 0.0 24.534815 1 TACATGG 11855 0.0 24.355053 2 GTATCAA 15475 0.0 24.243708 1 ACATGGG 11695 0.0 23.515327 3 GGTATCA 11435 0.0 23.110767 1 TCAACGC 17555 0.0 21.039896 4 ATCAACG 17535 0.0 21.036995 3 CAACGCA 17690 0.0 20.799335 5 AACGCAG 18025 0.0 20.399742 6 TATCAAC 18250 0.0 20.31884 2 CATGGGA 6490 0.0 17.952913 4 CATGGGG 6240 0.0 17.840628 4 ACGCAGA 20095 0.0 17.82916 7 CGCAGAG 19800 0.0 17.761473 8 AGAGTAC 14275 0.0 17.568958 10-11 GAGTACT 8495 0.0 17.563894 12-13 AGTACTT 9410 0.0 16.056427 12-13 GCAGAGT 21300 0.0 15.4927635 9 GTACTTT 9420 0.0 15.488888 14-15 GTATAGG 1350 0.0 15.031325 1 >>END_MODULE