FastQCFastQC Report
Fri 27 May 2016
SRR939669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944142
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139290.47310897368401394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92780.31513425643192483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68020.23103505197779184No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51550.17509345676940852No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG139250.028.4959491
TACATGG143100.027.1327062
GTATCAA222600.026.8242471
ACATGGG143150.026.128493
GGTATCA157500.022.84341
CATGGGA99500.022.6590964
ATCAACG262000.022.6095923
CAACGCA263450.022.484775
TCAACGC262900.022.4776464
CATGGGG47650.022.412574
TATCAAC264250.022.3991172
GAGTACT111050.022.05161712-13
AACGCAG269600.021.9662536
AGTACTT117700.021.69277612-13
ACGCAGA292850.019.9954517
CGCAGAG292700.019.7473138
GTACTTT126250.019.35926414-15
AGAGTAC208000.019.33684510-11
GCAGAGT289050.018.3558469
CATTCCG4400.018.3358849
Produced by FastQC (version 0.11.5)