##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939669_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944142 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.198762491754813 31.0 30.0 33.0 26.0 34.0 2 30.327783442510587 31.0 30.0 34.0 26.0 34.0 3 30.36519298321888 31.0 30.0 34.0 26.0 34.0 4 33.962421309841716 35.0 35.0 37.0 28.0 37.0 5 33.97822591437505 35.0 35.0 37.0 28.0 37.0 6 34.08681238880462 36.0 35.0 37.0 29.0 37.0 7 33.90329542528859 36.0 35.0 37.0 28.0 37.0 8 33.90215995016545 36.0 35.0 37.0 28.0 37.0 9 35.16441802059819 38.0 35.0 39.0 28.0 39.0 10-11 35.05776215956975 37.0 34.5 39.0 28.0 39.0 12-13 34.91155283950299 37.0 34.0 39.0 27.0 39.0 14-15 35.58232585248945 38.0 34.0 40.0 27.0 41.0 16-17 35.32630830985734 38.0 34.0 40.0 27.0 41.0 18-19 35.33000684070265 38.0 34.0 40.0 27.0 41.0 20-21 35.474529761132445 38.0 34.0 40.0 27.0 41.0 22-23 35.32174755837185 38.0 34.0 40.0 26.5 41.0 24-25 35.196808951470416 38.0 34.0 40.0 26.0 41.0 26-27 35.31463309174625 38.0 34.0 40.0 26.0 41.0 28-29 35.187240629018575 38.0 34.0 40.0 25.0 41.0 30-31 34.950305046427786 38.0 34.0 40.0 25.0 41.0 32-33 34.701258125457265 38.0 33.5 40.0 24.0 41.0 34-35 34.45619165108205 38.0 33.0 40.0 24.0 41.0 36-37 34.14672118396463 38.0 33.0 40.0 23.5 41.0 38-39 33.97877361214235 38.0 33.0 40.0 22.5 41.0 40-41 33.69800301751749 38.0 32.5 40.0 20.5 41.0 42-43 33.36278956653585 37.5 32.0 40.0 18.5 41.0 44-45 33.0727867405852 37.0 31.5 40.0 17.0 41.0 46-47 32.580225580152046 37.0 31.0 40.0 14.0 41.0 48-49 32.199136964181754 37.0 30.5 40.0 9.5 41.0 50-51 31.58606870864245 36.0 30.0 38.5 9.0 40.5 52-53 31.640338679316418 36.5 30.0 39.0 8.0 40.0 54-55 31.683401649784557 36.5 30.0 39.0 8.0 40.0 56-57 31.558804059043347 36.0 30.0 39.0 7.0 40.0 58-59 31.172801617585023 36.0 29.5 39.0 2.0 40.0 60-61 30.75696382851099 35.0 28.5 39.0 2.0 40.0 62-63 30.36558630663874 35.0 28.0 38.5 2.0 40.0 64-65 29.768421326145273 34.5 27.5 38.0 2.0 40.0 66-67 29.230564965956127 34.0 26.5 38.0 2.0 40.0 68-69 28.718567243020207 34.0 26.0 37.0 2.0 39.0 70-71 28.173492141343726 33.5 26.0 36.5 2.0 39.0 72-73 27.445528102924385 33.0 24.5 36.0 2.0 39.0 74-75 26.985180741961493 32.0 24.0 36.0 2.0 38.0 76-77 26.608557603539502 32.0 22.5 35.0 2.0 37.5 78-79 26.038673915864113 32.0 21.5 35.0 2.0 37.0 80-81 25.36822595513396 31.0 19.5 35.0 2.0 36.0 82-83 24.711278701910437 31.0 17.5 34.0 2.0 36.0 84-85 24.049416094739996 30.5 11.5 34.0 2.0 35.0 86-87 23.381598951409273 30.0 6.5 34.0 2.0 35.0 88-89 22.588734680596247 29.5 2.0 34.0 2.0 35.0 90-91 21.356295993875293 28.5 2.0 33.5 2.0 35.0 92-93 19.692059859884477 26.0 2.0 33.0 2.0 34.0 94-95 19.462936570314884 26.0 2.0 32.0 2.0 34.0 96-97 18.83834950895711 25.0 2.0 32.0 2.0 34.0 98-99 17.86088442744949 24.5 2.0 32.0 2.0 34.0 100-101 16.204879723872015 17.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7970309911706863 1101 2 0.8800527188288143 1101 3 0.8743836421019218 1101 4 0.9155255409931229 1101 5 0.8069901761800722 1101 6 0.7617234993215405 1101 7 0.6466823736349667 1101 8 0.8062724870984326 1101 9 0.9938648754805115 1101 10-11 0.7926715882074191 1101 12-13 0.5833528678088129 1101 14-15 0.7917961431180203 1101 16-17 0.7158176445409339 1101 18-19 0.721258038210209 1101 20-21 0.9751825578363906 1101 22-23 0.9637154946112148 1101 24-25 0.9684303075223468 1101 26-27 0.8644738888058754 1101 28-29 0.8229549928113258 1101 30-31 0.9579065732499359 1101 32-33 0.7454251115571324 1101 34-35 0.7674745416885784 1101 36-37 0.6082395928587374 1101 38-39 0.31700744893529986 1101 40-41 0.6661378606285453 1101 42-43 0.7759602057693726 1101 44-45 0.58623465443344 1101 46-47 0.7503660151360094 1101 48-49 0.5343043601022686 1101 50-51 0.49409450732283133 1101 52-53 0.6552513252138112 1101 54-55 0.745716515808553 1101 56-57 0.5112379185276339 1101 58-59 0.3900195804589224 1101 60-61 0.13279561258986305 1101 62-63 0.2301419947743426 1101 64-65 0.24948138516495533 1101 66-67 0.06372938499214342 1101 68-69 0.13407675443169964 1101 70-71 0.08432438540404874 1101 72-73 0.0881091556494944 1101 74-75 -0.21210434065906725 1101 76-77 -0.5053335462255895 1101 78-79 -0.41631405151757406 1101 80-81 -0.46631854190037103 1101 82-83 -0.41745283472805994 1101 84-85 -0.766045015592244 1101 86-87 -1.103652368389529 1101 88-89 -1.4162774395257038 1101 90-91 -4.671951098993869 1101 92-93 -9.60168320424204 1101 94-95 -7.8948721384301095 1101 96-97 -4.602836769095438 1101 98-99 -3.604591056424841 1101 100-101 -2.9826217122555025 1102 1 0.8204627229370196 1102 2 0.7643655428868854 1102 3 0.6850054879366958 1102 4 0.9066973349786025 1102 5 0.7980866158620614 1102 6 0.7938334198192933 1102 7 0.8192299021520739 1102 8 0.8335262029643147 1102 9 0.9615720998151147 1102 10-11 0.8120813704404028 1102 12-13 0.7376491000936412 1102 14-15 0.9244701839061378 1102 16-17 1.1493316438496137 1102 18-19 1.0762169044390362 1102 20-21 1.1251523124164038 1102 22-23 1.0630158748393583 1102 24-25 1.0005011682205875 1102 26-27 1.0019159904304829 1102 28-29 1.1686782904262571 1102 30-31 1.0135641950957748 1102 32-33 0.9379340987676343 1102 34-35 1.0262735392689422 1102 36-37 1.0339438790668254 1102 38-39 1.0939267368717012 1102 40-41 0.9097992847557563 1102 42-43 0.9011014086729361 1102 44-45 0.7241891998879879 1102 46-47 0.6901956037983012 1102 48-49 0.719962846102959 1102 50-51 0.7536511958814209 1102 52-53 0.8149505993237298 1102 54-55 0.8778063879136369 1102 56-57 0.9644088483582607 1102 58-59 0.8452114228370782 1102 60-61 0.6544043232708212 1102 62-63 0.7212346321749656 1102 64-65 0.5765797259876315 1102 66-67 0.5774957175189321 1102 68-69 0.43386451799893067 1102 70-71 0.5053095219405144 1102 72-73 0.39531568866020805 1102 74-75 0.2930258010623277 1102 76-77 -0.08167022095770093 1102 78-79 -0.2860001904736791 1102 80-81 -0.40013257577524186 1102 82-83 -0.532243190759857 1102 84-85 -0.7926373073309243 1102 86-87 -1.172295472433774 1102 88-89 -1.7872890192007809 1102 90-91 -5.592992232765042 1102 92-93 -10.847822160342972 1102 94-95 -9.252298858098275 1102 96-97 -5.754851632558971 1102 98-99 -4.2454882219969114 1102 100-101 -3.616507470983466 1103 1 0.7706440433277848 1103 2 0.7838463468194377 1103 3 0.7747952956649016 1103 4 0.7581303509701414 1103 5 0.6543790681052357 1103 6 0.5413557893047027 1103 7 0.6461566646435202 1103 8 0.5932897522145169 1103 9 0.5754639547090221 1103 10-11 0.6162329478157957 1103 12-13 0.59419268792265 1103 14-15 0.6086203923561371 1103 16-17 0.7885866995399837 1103 18-19 0.541118777377875 1103 20-21 0.7065971509454343 1103 22-23 0.5641096462288147 1103 24-25 0.5060526584825524 1103 26-27 0.506060723178912 1103 28-29 0.7049373906689311 1103 30-31 0.8202028610157512 1103 32-33 0.5764661129546838 1103 34-35 0.47831784267258826 1103 36-37 0.5438374762294202 1103 38-39 0.639385637997961 1103 40-41 0.3986487651863442 1103 42-43 0.32015050331546036 1103 44-45 0.25687976052515893 1103 46-47 0.4826731098651251 1103 48-49 0.23956834669408522 1103 50-51 0.2428012176597747 1103 52-53 0.2817871371869636 1103 54-55 0.2509417248559558 1103 56-57 0.20748693932957352 1103 58-59 0.11671661853118209 1103 60-61 0.128397354196089 1103 62-63 0.2163854006399042 1103 64-65 0.19890092566406636 1103 66-67 0.12375780450791751 1103 68-69 0.039060709897380974 1103 70-71 0.29765162884114105 1103 72-73 0.2756472175009783 1103 74-75 0.1025716911749548 1103 76-77 -0.096185559144498 1103 78-79 -0.12238286734505976 1103 80-81 -0.0797929578298735 1103 82-83 -0.1556691132289032 1103 84-85 -0.4689942235153559 1103 86-87 -0.9033212953446075 1103 88-89 -1.2037112704608113 1103 90-91 -2.733336081934354 1103 92-93 -9.448390886620118 1103 94-95 -5.738067883050052 1103 96-97 -3.2165971368835304 1103 98-99 -2.1921022472598075 1103 100-101 -1.7906834401204215 1104 1 0.8741843292439917 1104 2 0.7973672674495873 1104 3 0.7561986389591162 1104 4 0.8683615379842706 1104 5 0.800070848431659 1104 6 0.633517578479065 1104 7 0.8752868127211784 1104 8 0.7810231233880671 1104 9 0.847030094395059 1104 10-11 0.6640966451385992 1104 12-13 0.6694606300271246 1104 14-15 0.7755694694444486 1104 16-17 0.878392865825468 1104 18-19 0.7245328689623207 1104 20-21 0.8248874688588828 1104 22-23 0.720277392454534 1104 24-25 0.7087906483881099 1104 26-27 0.6297036228522686 1104 28-29 0.7229570536684378 1104 30-31 0.8028805291682062 1104 32-33 0.6782868950228718 1104 34-35 0.7750887646939191 1104 36-37 0.8225925610081859 1104 38-39 0.7811723638734236 1104 40-41 0.6248337760097584 1104 42-43 0.7582188021195932 1104 44-45 0.6964988820757156 1104 46-47 0.5952581807877024 1104 48-49 0.6780362632007666 1104 50-51 0.5556239724273659 1104 52-53 0.6893488501244178 1104 54-55 0.7670850279727617 1104 56-57 0.7322197623764879 1104 58-59 0.7595074317062576 1104 60-61 0.6721347987573942 1104 62-63 0.559534582901744 1104 64-65 0.7540300832184741 1104 66-67 0.6177579742324077 1104 68-69 0.5484880705980935 1104 70-71 0.7452811898390124 1104 72-73 0.7629387485271693 1104 74-75 0.6603479247381117 1104 76-77 0.47279109956459564 1104 78-79 0.2540222288414249 1104 80-81 0.20582936066230317 1104 82-83 0.12958558480062266 1104 84-85 0.051064220138894 1104 86-87 -0.12549552170699485 1104 88-89 -0.3278995143966412 1104 90-91 -0.7718266747458244 1104 92-93 -4.272516666378941 1104 94-95 -1.6838770793350335 1104 96-97 -0.995911129972125 1104 98-99 -0.8654034720584995 1104 100-101 -0.5868888457063051 1105 1 0.9444205837537751 1105 2 0.795559197221646 1105 3 0.8613805266675634 1105 4 0.7836794258257243 1105 5 0.7119908111895725 1105 6 0.7185966347910977 1105 7 0.8591617358262411 1105 8 0.7543925349962919 1105 9 0.9860519907489902 1105 10-11 0.9219648679926067 1105 12-13 0.7208035897757341 1105 14-15 0.8937715410357114 1105 16-17 1.0384309807771714 1105 18-19 0.8556979727912761 1105 20-21 0.8713133088841545 1105 22-23 0.8455693854051347 1105 24-25 0.8442234214275004 1105 26-27 0.7847238233610128 1105 28-29 0.8907511664255168 1105 30-31 0.8896117875850891 1105 32-33 0.9293052334271223 1105 34-35 0.9275090849093957 1105 36-37 0.9003998498172763 1105 38-39 0.9286568601907845 1105 40-41 0.8375646686561069 1105 42-43 0.9746396128000967 1105 44-45 0.8530632161239637 1105 46-47 0.9478879877606161 1105 48-49 0.8221664775090431 1105 50-51 0.6920449493671974 1105 52-53 0.6444827304509602 1105 54-55 0.8018831060148983 1105 56-57 0.6655321054692465 1105 58-59 0.5666850548628553 1105 60-61 0.4443636713528676 1105 62-63 0.5245962235488264 1105 64-65 0.5973659935714437 1105 66-67 0.6227405585674539 1105 68-69 0.4409120485188538 1105 70-71 0.6112034303497609 1105 72-73 0.9496396646160825 1105 74-75 0.841195473244273 1105 76-77 0.37942713294946984 1105 78-79 0.4698663274664625 1105 80-81 0.4300103256314678 1105 82-83 0.565369996325721 1105 84-85 0.5798759231332262 1105 86-87 0.14840217892645313 1105 88-89 0.05653362441327303 1105 90-91 -1.0426236388504364 1105 92-93 -7.3797227614460255 1105 94-95 -3.035004317601281 1105 96-97 -1.3468336614367047 1105 98-99 -0.576128861520985 1105 100-101 -0.3400560859676638 1106 1 0.847973694723855 1106 2 0.7821210287803027 1106 3 0.8343095631718072 1106 4 0.8662584635052539 1106 5 0.785967493605007 1106 6 0.7263976831421743 1106 7 0.7944023159451774 1106 8 0.8628051998295234 1106 9 0.9292962377461507 1106 10-11 0.8322851321958851 1106 12-13 0.9296961220372069 1106 14-15 0.9397429111788611 1106 16-17 1.1365479447900455 1106 18-19 0.8884332053794779 1106 20-21 0.787628801444626 1106 22-23 0.9144641507014271 1106 24-25 0.8811030490983285 1106 26-27 0.8067749245997717 1106 28-29 0.9126124597052581 1106 30-31 0.847811843998592 1106 32-33 0.8228149765105854 1106 34-35 0.7427030063535867 1106 36-37 0.8566711517940959 1106 38-39 0.8794753262133952 1106 40-41 0.7700814164171987 1106 42-43 0.8467032895506819 1106 44-45 0.7258036199506819 1106 46-47 0.8829981116352883 1106 48-49 0.7974550091123618 1106 50-51 0.5897327005836068 1106 52-53 0.7070665867845491 1106 54-55 0.7854772343086154 1106 56-57 0.8145875317438396 1106 58-59 0.8558383820847233 1106 60-61 0.8061848875341404 1106 62-63 0.6990465444006695 1106 64-65 0.5781502589029941 1106 66-67 0.5664204824092138 1106 68-69 0.5941842672152298 1106 70-71 0.6930211206865948 1106 72-73 0.7261683532370107 1106 74-75 0.6706841082723578 1106 76-77 0.470542945186498 1106 78-79 0.3859935399338461 1106 80-81 0.36532823927177915 1106 82-83 0.6111517935661581 1106 84-85 0.4429175516032444 1106 86-87 0.21427506048158662 1106 88-89 0.3761655262537644 1106 90-91 0.02418958541677796 1106 92-93 -3.646505545922908 1106 94-95 -1.213681303239344 1106 96-97 -0.24931244760599114 1106 98-99 -0.20097725021007662 1106 100-101 0.01810694907922894 1107 1 0.46012366304677954 1107 2 0.5846461441753625 1107 3 0.5305141970753269 1107 4 0.5321101242890336 1107 5 0.43598830996847937 1107 6 0.53586324563652 1107 7 0.283311266981471 1107 8 0.28541358067846545 1107 9 0.34809703419831806 1107 10-11 0.2574512105740183 1107 12-13 0.39258575005784735 1107 14-15 0.43076653322610525 1107 16-17 0.5300410210865465 1107 18-19 0.516755678001438 1107 20-21 0.4647699454395564 1107 22-23 0.39889645226718073 1107 24-25 0.30150104891731644 1107 26-27 0.3348908174407441 1107 28-29 0.2846995264081258 1107 30-31 0.2869124413377264 1107 32-33 0.16485679024938804 1107 34-35 0.19648864306278568 1107 36-37 0.18675645363136795 1107 38-39 0.195267705614242 1107 40-41 0.12139556249376682 1107 42-43 -0.053259603018787516 1107 44-45 -0.07829946630147333 1107 46-47 -0.07560289298051259 1107 48-49 -0.031396018129925096 1107 50-51 -0.08541346649033343 1107 52-53 -0.06669936488528805 1107 54-55 0.14098474630065283 1107 56-57 -0.07507623873312852 1107 58-59 -0.20409124955614644 1107 60-61 -0.2180677597642564 1107 62-63 -0.19772194143515165 1107 64-65 -0.2546708108770801 1107 66-67 -0.2374916363345676 1107 68-69 -0.09723056670638996 1107 70-71 0.06474163838050728 1107 72-73 -0.09396061771058939 1107 74-75 -0.14233222804766754 1107 76-77 -0.3042891663168028 1107 78-79 -0.4250662844846538 1107 80-81 -0.39171561085040096 1107 82-83 -0.26531417620486053 1107 84-85 -0.36079454903935115 1107 86-87 -0.5618859043116089 1107 88-89 -0.6911244999929558 1107 90-91 -0.7010925668141965 1107 92-93 -4.097846617513097 1107 94-95 -1.8070379418491704 1107 96-97 -1.0175966814733108 1107 98-99 -0.8157761007847917 1107 100-101 -0.643299286779218 1108 1 0.33824315016007134 1108 2 0.45831007986710404 1108 3 0.14739150821663927 1108 4 -0.1773773560006191 1108 5 -0.9510476584139695 1108 6 -0.06282675960270012 1108 7 0.044443659786274736 1108 8 0.2272949414582257 1108 9 0.29773281984083155 1108 10-11 0.41722937897316825 1108 12-13 0.41121392870764595 1108 14-15 0.3882257141361123 1108 16-17 0.5150988043650884 1108 18-19 0.3959442063881369 1108 20-21 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2312 82-83 1.031920304659451 2312 84-85 1.0021209731482834 2312 86-87 0.9568278538523458 2312 88-89 1.2181035107498879 2312 90-91 1.840197337354759 2312 92-93 2.7363145556542072 2312 94-95 2.077252938941893 2312 96-97 1.6665028424234123 2312 98-99 1.5692432790601707 2312 100-101 1.5816968982906694 2313 1 -0.09601040987811871 2313 2 -0.8213827955157278 2313 3 -0.18398809097509883 2313 4 0.1309634158617854 2313 5 -0.5563279386045394 2313 6 -0.1917209341291155 2313 7 -1.0519844720734497 2313 8 -0.9232136866380287 2313 9 -0.9902222000609697 2313 10-11 -1.799443919635074 2313 12-13 -0.39089162312367165 2313 14-15 -0.01780355722262783 2313 16-17 -1.231208966717027 2313 18-19 -0.8934005338847157 2313 20-21 -1.2543480818609396 2313 22-23 -0.9297028364369808 2313 24-25 -0.11633830457427763 2313 26-27 -0.6105014929535386 2313 28-29 -2.1321817974412625 2313 30-31 -0.31169997072601774 2313 32-33 -0.10867500340651759 2313 34-35 0.19467198468969116 2313 36-37 0.22395739714725948 2313 38-39 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-0.6342569361636734 2314 62-63 -0.7234661676447942 2314 64-65 -0.9106216934108105 2314 66-67 -0.7098411079851807 2314 68-69 -0.7674567617126833 2314 70-71 -0.8192922579400772 2314 72-73 -0.7656034281585384 2314 74-75 -0.46794705012613846 2314 76-77 -0.36975594811778123 2314 78-79 -0.3334088871054206 2314 80-81 -0.476570448930584 2314 82-83 -0.47297609602064483 2314 84-85 -0.4115073084574341 2314 86-87 -0.3137781805558859 2314 88-89 0.08369340729465691 2314 90-91 0.8030743437810912 2314 92-93 1.5954576639219837 2314 94-95 0.8937805606242684 2314 96-97 0.8693210609185549 2314 98-99 0.7834339363899296 2314 100-101 0.8139747865359688 2315 1 -7.386520024449592 2315 2 -2.9768553415574317 2315 3 -1.4090162123947572 2315 4 -0.9349775000025531 2315 5 -1.42774856360478 2315 6 -1.6882310012691235 2315 7 -3.1112281844792733 2315 8 -2.683201183080616 2315 9 -3.3915834003407 2315 10-11 -2.2438088814080928 2315 12-13 -2.7937858414061196 2315 14-15 -4.3247101894755495 2315 16-17 -4.135627080193565 2315 18-19 -2.961895450018588 2315 20-21 -4.170660600130688 2315 22-23 -5.666117188420223 2315 24-25 -3.50255863517328 2315 26-27 -4.438093924864567 2315 28-29 -2.763212697987015 2315 30-31 -2.6910716302817193 2315 32-33 -1.8623516883651448 2315 34-35 -2.494348121401334 2315 36-37 -3.0241172558095144 2315 38-39 -2.2461018758368745 2315 40-41 -3.5370490678887947 2315 42-43 -7.647679803259415 2315 44-45 -4.1439577731140815 2315 46-47 -2.7104839055362433 2315 48-49 -5.489100583455951 2315 50-51 -6.605651617229437 2315 52-53 -6.707611796211552 2315 54-55 -4.42822630423062 2315 56-57 -3.669419248754977 2315 58-59 -4.020929544329132 2315 60-61 -2.883912682876666 2315 62-63 -3.2276550814146567 2315 64-65 -2.1788466033701397 2315 66-67 -2.7172383542678062 2315 68-69 -2.1335622646294716 2315 70-71 -1.77602649324405 2315 72-73 -2.1136944249407428 2315 74-75 -2.5913993101306225 2315 76-77 -2.6612247125863604 2315 78-79 -1.7143852438746237 2315 80-81 -1.4703758687863733 2315 82-83 -1.8257311649234325 2315 84-85 -1.761504923977288 2315 86-87 -2.0746710071514727 2315 88-89 -1.6328871381276606 2315 90-91 -0.6397727081326181 2315 92-93 0.19803437950234937 2315 94-95 -0.20611868302249192 2315 96-97 -0.3772964043655378 2315 98-99 -0.3591372205521104 2315 100-101 -0.6397831445152953 2316 1 -4.224534900929918 2316 2 -2.8318712490401268 2316 3 -4.48331837019936 2316 4 -4.35362358965984 2316 5 -4.151531032059555 2316 6 -4.466594049452318 2316 7 -2.959808497331629 2316 8 -1.522323484618191 2316 9 -1.885228803702148 2316 10-11 -3.002875653819622 2316 12-13 -3.8908745292296025 2316 14-15 -2.7940195928546956 2316 16-17 -2.6252125786268508 2316 18-19 -2.9516574245233826 2316 20-21 -5.340405492153984 2316 22-23 -6.3776966130599675 2316 24-25 -4.224796963141436 2316 26-27 -3.8985653965271823 2316 28-29 -4.643563781796452 2316 30-31 -6.125537567936291 2316 32-33 -4.435023981551524 2316 34-35 -6.082088806355749 2316 36-37 -9.314174282216872 2316 38-39 -6.137067718817818 2316 40-41 -5.310896540877145 2316 42-43 -4.422751095067277 2316 44-45 -3.235179511426516 2316 46-47 -5.424596440556186 2316 48-49 -2.86092923052 2316 50-51 -4.294042488298228 2316 52-53 -5.195279386888828 2316 54-55 -6.773158087430318 2316 56-57 -4.687812630711235 2316 58-59 -5.452964234999765 2316 60-61 -5.8114489431787675 2316 62-63 -5.698858007679533 2316 64-65 -4.791281871595167 2316 66-67 -6.0461834165125765 2316 68-69 -4.741402518288435 2316 70-71 -5.509084564128305 2316 72-73 -7.422591202125677 2316 74-75 -7.485141408301395 2316 76-77 -6.860834584828748 2316 78-79 -5.8398006539663 2316 80-81 -5.8362057372056135 2316 82-83 -4.660614571557183 2316 84-85 -6.290370828408104 2316 86-87 -5.072477173275686 2316 88-89 -4.97105938500793 2316 90-91 -5.3161476201334 2316 92-93 -4.219053642639313 2316 94-95 -4.3322497513482965 2316 96-97 -5.078506125210559 2316 98-99 -6.065997946541485 2316 100-101 -5.034407321375088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14901.0 3 5932.0 4 6075.0 5 6914.0 6 8031.0 7 9611.0 8 11772.0 9 14456.0 10 17386.0 11 21297.0 12 25278.0 13 29321.0 14 31587.0 15 36343.0 16 40807.0 17 45270.0 18 40976.0 19 37430.0 20 39405.0 21 42810.0 22 46912.0 23 52563.0 24 59019.0 25 66424.0 26 75056.0 27 85595.0 28 97266.0 29 110292.0 30 126121.0 31 145210.0 32 167732.0 33 195289.0 34 231182.0 35 278615.0 36 324680.0 37 297207.0 38 97664.0 39 1713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34115152510324 19.610072912448967 13.620590772038362 22.428184790409432 2 15.010245988730262 21.36180699324856 40.86984993290042 22.758097085120756 3 18.779413434659592 26.804142989156343 30.42534860933655 23.991094966847516 4 11.880162725187894 17.262572016775724 39.28256076269357 31.574704495342814 5 12.682406899001968 38.69601655053302 34.6729586797652 13.948617870699811 6 29.447343901005496 37.94650035234261 18.78171380941681 13.824441937235084 7 27.01576999637921 34.00801334083557 22.14384915744328 16.832367505341946 8 25.661720935086972 34.645153986508056 20.895140812773523 18.79798426563144 9 27.09032271881585 16.469255655729427 21.082972454696545 35.35744917075817 10-11 24.11752576934054 27.00897505177326 28.612123042810957 20.261376136075242 12-13 25.81174358392928 24.92725130007651 28.497942264415155 20.763062851579054 14-15 22.72432025183495 25.703810723962583 27.5538823676606 24.01798665654186 16-17 21.42889562080883 29.15702903045564 27.819016028719883 21.595059320015647 18-19 21.17262813720712 27.707990761198186 30.500948478227496 20.61843262336719 20-21 22.336565746619804 26.7487002257572 30.167020203900623 20.747713823722375 22-23 21.85721737722913 26.950966463337917 29.98630948454155 21.205506674891403 24-25 21.765466380863817 27.0015666549434 30.218974551891108 21.013992412301675 26-27 21.610005396527413 27.043099256476555 30.389286404917797 20.957608942078238 28-29 21.139188557839997 27.32734791008225 30.497953221993733 21.035510310084018 30-31 21.40844394902704 27.07216702445343 30.364258786128328 21.155130240391205 32-33 21.316541593146113 27.228895172091246 30.15880439635196 21.29575883841068 34-35 21.901993913415403 27.09138975314384 29.96358083006456 21.043035503376192 36-37 21.933124384237082 26.817594377864523 29.97225633560837 21.27702490229002 38-39 21.427894840935462 27.33992489221303 30.10748171579612 21.12469855105539 40-41 21.562033793622632 27.07582946367338 30.220929000674595 21.141207742029398 42-43 21.361476720062576 27.258255960276163 29.71346461245451 21.66680270720675 44-45 21.572511877673985 27.328546103330574 29.504087769813044 21.594854249182397 46-47 21.8215327744675 27.44595071704033 29.03640881876951 21.69610768972266 48-49 21.793874314967482 27.61834745781452 28.9254417224394 21.6623365047786 50-51 21.56532250889415 27.725824953222045 28.966829372354574 21.742023165529233 52-53 21.889500491508922 27.961821886965538 28.560224219259595 21.58845340226594 54-55 21.444228934833397 27.583196284078227 28.82209206205384 22.15048271903454 56-57 21.711184563538666 28.0966760178202 28.43193995760092 21.760199461040212 58-59 22.006103373010642 27.51964804845966 28.510030431836174 21.964218146693522 60-61 21.849235074411126 27.723918693623684 28.88171422779961 21.54513200416558 62-63 21.726983215752 27.611281686423776 28.888635330991686 21.77309976683254 64-65 21.49352455554641 27.704976397594944 28.766336529966996 22.035162516891653 66-67 21.54841676141011 27.93927146572385 28.292080967297956 22.220230805568093 68-69 21.529450914236055 27.6988417290421 28.669097918506303 22.102609438215545 70-71 21.53873101116755 27.751371923481788 28.597109395061242 22.112787670289418 72-73 21.74508117873099 27.520070355750725 28.48599901848965 22.248849447028636 74-75 21.436705951364644 28.119142299027583 28.21500912053292 22.22914262907485 76-77 21.631054964768268 27.636476173495012 28.463047173907423 22.269421687829297 78-79 21.50649903024941 27.78029194596618 28.412943618360607 22.300265405423797 80-81 21.514042648326637 27.992415715951413 28.249671581921103 22.243870053800848 82-83 21.627285486320726 27.872592777605465 28.15723911091662 22.34288262515719 84-85 21.46581772093418 27.73620765115999 28.035740499668215 22.76223412823762 86-87 21.478322860952677 27.638256602498778 28.310315869046 22.57310466750255 88-89 21.628500545959444 27.500244142037246 28.143690263887766 22.727565048115547 90-91 21.214783276198286 27.576594224467517 28.57939773553137 22.62922476380283 92-93 21.050762580868536 27.62136640041411 28.87355467817677 22.45431634054058 94-95 21.442595483641778 27.295064563152387 28.271236016534484 22.991103936671355 96-97 21.52096268932272 27.251734341579787 28.199313580188097 23.027989388909404 98-99 21.412611403201687 27.30492537153405 27.878596442619873 23.403866782644393 100-101 21.353260964840988 27.179186141250465 28.31990983411663 23.147643059791914 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3526.0 1 2206.5 2 950.0 3 990.5 4 862.5 5 868.5 6 1286.0 7 2276.5 8 3500.0 9 3985.5 10 3613.0 11 3257.0 12 3314.5 13 3609.5 14 3869.0 15 4062.0 16 4392.5 17 4708.0 18 5001.0 19 5441.5 20 6065.5 21 7017.0 22 8473.0 23 10156.5 24 11982.0 25 14749.0 26 19548.5 27 24625.0 28 28850.0 29 33759.5 30 40208.0 31 47307.5 32 54354.5 33 62459.0 34 72324.5 35 79841.5 36 86734.0 37 94283.0 38 96619.5 39 95055.0 40 92854.5 41 94368.0 42 97089.5 43 98553.5 44 100863.5 45 102113.5 46 101675.0 47 105024.0 48 116886.0 49 117812.5 50 105349.0 51 96171.5 52 90099.5 53 86585.0 54 82774.0 55 76390.5 56 68867.0 57 61053.5 58 54698.5 59 49071.0 60 42618.5 61 35641.0 62 28788.5 63 22519.5 64 17049.5 65 12855.5 66 9390.0 67 6864.0 68 4834.5 69 3112.5 70 2145.5 71 1572.0 72 1151.5 73 818.0 74 616.0 75 496.5 76 354.0 77 208.5 78 117.0 79 82.5 80 67.5 81 42.0 82 28.0 83 26.0 84 18.5 85 12.0 86 7.0 87 4.5 88 6.0 89 5.0 90 3.0 91 3.0 92 3.5 93 3.0 94 2.0 95 2.0 96 1.0 97 0.5 98 1.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09065459478517 2 0.10159156725456857 3 0.09795723168243922 4 0.10145570424252635 5 0.09670049882104871 6 0.08161970448436251 7 0.09500221117052099 8 0.09113011532731777 9 0.08420110171316465 10-11 0.09206417353510801 12-13 0.09104520094479139 14-15 0.09681937895658566 16-17 0.09393228995068853 18-19 0.09685334470959621 20-21 0.10700910485975201 22-23 0.11616287529609645 24-25 0.1399389024034846 26-27 0.14566213178576307 28-29 0.14528850850264696 30-31 0.14635842972247942 32-33 0.14280900853287648 34-35 0.13822363187645162 36-37 0.14236745374373924 38-39 0.13475912506937504 40-41 0.13088702922617182 42-43 0.13049642306655046 44-45 0.12027273141037355 46-47 0.1406182174636957 48-49 0.1590616213484268 50-51 0.16950609039917233 52-53 0.1777427855042318 54-55 0.17368387801947055 56-57 0.14331849482803477 58-59 0.1346572278103434 60-61 0.13097194360869824 62-63 0.12791502583774833 64-65 0.11344561505525208 66-67 0.12720174502452666 68-69 0.12504491970835646 70-71 0.1333835120724476 72-73 0.16284880280910363 74-75 0.1755689773115563 76-77 0.18049401149808672 78-79 0.17804847728132678 80-81 0.18455291898284798 82-83 0.18366980940457356 84-85 0.16070896036943869 86-87 0.16111654940556536 88-89 0.1793052101427173 90-91 0.26574805155457853 92-93 0.5254162333202679 94-95 0.18049401149808672 96-97 0.19384255243123463 98-99 0.2113179323551649 100-101 0.21892626102952914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.560621586124874 #Duplication Level Percentage of deduplicated Percentage of total 1 75.35222156951549 37.34502938880594 2 12.683840726260012 12.572380609857033 3 4.808087178812244 7.148753676666371 4 2.2926649230814165 4.5450359470648065 5 1.2738572303448419 3.156657807393491 6 0.7982842456863395 2.373807805117549 7 0.5335564773786429 1.8510373469132102 8 0.37099471816693425 1.470938307001798 9 0.2725761354564659 1.2158138432489598 >10 1.40357111651313 12.612619505938092 >50 0.12364753346273975 4.281246357128403 >100 0.07991072215670031 7.417790567139162 >500 0.0051780341672266975 1.773393787978928 >1k 0.001329495259124582 1.0160332283742752 >5k 2.099203040723024E-4 0.7364142809928232 >10k+ 6.997343469076747E-5 0.4830475403790457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13929 0.47310897368401394 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9278 0.31513425643192483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6802 0.23103505197779184 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5155 0.17509345676940852 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.3965753010554516E-5 7 0.0 0.0 0.0 0.0 3.3965753010554516E-5 8 0.0 0.0 0.0 0.0 3.3965753010554516E-5 9 0.0 0.0 0.0 0.0 3.3965753010554516E-5 10-11 0.0 0.0 0.0 0.0 3.3965753010554516E-5 12-13 0.0 0.0 0.0 0.0 6.793150602110903E-5 14-15 0.0 0.0 0.0 0.0 6.793150602110903E-5 16-17 3.3965753010554516E-5 0.0 0.0 1.6982876505277258E-5 6.793150602110903E-5 18-19 3.3965753010554516E-5 0.0 0.0 1.5284588854749532E-4 6.793150602110903E-5 20-21 3.3965753010554516E-5 0.0 0.0 3.5664040661082246E-4 6.793150602110903E-5 22-23 3.3965753010554516E-5 0.0 0.0 6.793150602110903E-4 6.793150602110903E-5 24-25 3.3965753010554516E-5 0.0 0.0 0.0012227671083799626 6.793150602110903E-5 26-27 3.3965753010554516E-5 0.0 0.0 0.0020039794276227162 6.793150602110903E-5 28-29 3.3965753010554516E-5 0.0 0.0 0.0045683937799195825 6.793150602110903E-5 30-31 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.012244653960304904 6.793150602110903E-5 32-33 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.028225540751770806 6.793150602110903E-5 34-35 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.0530205404494756 6.793150602110903E-5 36-37 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.09070554341468584 6.793150602110903E-5 38-39 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.14372608386416144 6.793150602110903E-5 40-41 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.20372658655730602 6.793150602110903E-5 42-43 5.094862951583177E-5 3.3965753010554516E-5 0.0 0.2669708186629585 6.793150602110903E-5 44-45 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.34454859853906505 6.793150602110903E-5 46-47 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.4358485426314356 6.793150602110903E-5 48-49 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.5355889763469289 6.793150602110903E-5 50-51 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.640950742185669 6.793150602110903E-5 52-53 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.7485032990935899 6.793150602110903E-5 54-55 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.8632226298867378 6.793150602110903E-5 56-57 6.793150602110903E-5 3.3965753010554516E-5 0.0 0.9990007275464295 6.793150602110903E-5 58-59 6.793150602110903E-5 3.3965753010554516E-5 0.0 1.1498426366663022 6.793150602110903E-5 60-61 6.793150602110903E-5 3.3965753010554516E-5 0.0 1.321896158541266 6.793150602110903E-5 62-63 6.793150602110903E-5 3.3965753010554516E-5 0.0 1.4939157146632194 6.793150602110903E-5 64-65 6.793150602110903E-5 3.3965753010554516E-5 0.0 1.6742398973962533 6.793150602110903E-5 66-67 6.793150602110903E-5 3.3965753010554516E-5 0.0 1.8758096586373891 6.793150602110903E-5 68-69 6.793150602110903E-5 3.3965753010554516E-5 0.0 2.1069466078742125 6.793150602110903E-5 70-71 6.793150602110903E-5 3.3965753010554516E-5 0.0 2.3645768444592687 6.793150602110903E-5 72-73 6.793150602110903E-5 3.3965753010554516E-5 0.0 2.6307155021734685 6.793150602110903E-5 74-75 6.793150602110903E-5 3.3965753010554516E-5 0.0 2.905362581016812 6.793150602110903E-5 76-77 6.793150602110903E-5 3.3965753010554516E-5 0.0 3.2017817075399218 6.793150602110903E-5 78-79 6.793150602110903E-5 3.3965753010554516E-5 0.0 3.5305192480525736 6.793150602110903E-5 80-81 6.793150602110903E-5 3.3965753010554516E-5 0.0 3.895685058669045 6.793150602110903E-5 82-83 6.793150602110903E-5 3.3965753010554516E-5 0.0 4.258337403562736 6.793150602110903E-5 84-85 8.491438252638629E-5 3.3965753010554516E-5 0.0 4.615674108110275 6.793150602110903E-5 86-87 1.0189725903166355E-4 3.3965753010554516E-5 0.0 4.998128487009119 6.793150602110903E-5 88-89 1.0189725903166355E-4 3.3965753010554516E-5 0.0 5.409810396373544 1.0189725903166355E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13925 0.0 28.495949 1 TACATGG 14310 0.0 27.132706 2 GTATCAA 22260 0.0 26.824247 1 ACATGGG 14315 0.0 26.12849 3 GGTATCA 15750 0.0 22.8434 1 CATGGGA 9950 0.0 22.659096 4 ATCAACG 26200 0.0 22.609592 3 CAACGCA 26345 0.0 22.48477 5 TCAACGC 26290 0.0 22.477646 4 CATGGGG 4765 0.0 22.41257 4 TATCAAC 26425 0.0 22.399117 2 GAGTACT 11105 0.0 22.051617 12-13 AACGCAG 26960 0.0 21.966253 6 AGTACTT 11770 0.0 21.692776 12-13 ACGCAGA 29285 0.0 19.995451 7 CGCAGAG 29270 0.0 19.747313 8 GTACTTT 12625 0.0 19.359264 14-15 AGAGTAC 20800 0.0 19.336845 10-11 GCAGAGT 28905 0.0 18.355846 9 CATTCCG 440 0.0 18.335884 9 >>END_MODULE