FastQCFastQC Report
Fri 27 May 2016
SRR939669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944142
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146500.4975982816046237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111570.37895590633875675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78460.26649529812081074No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54600.18545301143762769No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30390.10322192339907517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA198200.026.9518221
GGTATCA137450.026.491921
GTACATG145750.026.4376871
TACATGG146300.025.8182932
ACATGGG142350.024.8129863
ATCAACG234500.022.5132433
TCAACGC235450.022.4024034
CAACGCA237250.022.2721395
AACGCAG238100.022.2585896
CATGGGA96350.021.7023374
TATCAAC244500.021.612822
GAGTACT111600.021.30015212-13
CATGGGG47750.020.4160754
AGTACTT118200.020.30972312-13
ACGCAGA263400.019.9063767
CGCAGAG261750.019.744418
TAGGACG4100.019.5272164
GTACTTT124400.019.184114-15
AGAGTAC206550.019.04436510-11
ATGGGAG41850.018.6804755
Produced by FastQC (version 0.11.5)