##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939669_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944142 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.552213853815473 31.0 31.0 34.0 30.0 34.0 2 31.752092799871747 31.0 31.0 34.0 30.0 34.0 3 31.79233100849076 31.0 31.0 34.0 30.0 34.0 4 35.58197770352109 37.0 35.0 37.0 33.0 37.0 5 35.32039555157326 37.0 35.0 37.0 33.0 37.0 6 35.35002625552708 37.0 35.0 37.0 33.0 37.0 7 35.279129199610615 37.0 35.0 37.0 32.0 37.0 8 35.24546166591149 37.0 35.0 37.0 32.0 37.0 9 36.848792959035265 39.0 37.0 39.0 33.0 39.0 10-11 36.73204723141751 39.0 35.0 39.0 32.0 39.0 12-13 36.67652001839585 39.0 35.0 39.0 32.0 39.0 14-15 37.71503276676193 39.5 37.0 41.0 32.0 41.0 16-17 37.68441943357352 39.0 36.5 41.0 32.0 41.0 18-19 37.69844610076552 39.5 37.0 41.0 32.0 41.0 20-21 37.65179991318354 39.0 37.0 41.0 32.0 41.0 22-23 37.509304918037245 39.0 36.0 41.0 32.0 41.0 24-25 37.37863357134269 39.0 36.0 41.0 31.5 41.0 26-27 37.125052562002786 39.0 36.0 40.5 31.0 41.0 28-29 36.92713768561435 39.0 36.0 40.0 31.0 41.0 30-31 36.67393182801645 38.5 35.5 40.0 30.0 41.0 32-33 36.482527846822606 38.0 35.0 40.0 30.0 41.0 34-35 36.44459285591524 38.0 35.0 40.0 30.0 41.0 36-37 36.309942591084265 38.0 35.0 40.0 30.0 41.0 38-39 36.331228928495975 38.5 35.0 40.0 30.0 41.0 40-41 36.22786400927673 38.5 35.0 40.0 30.0 41.0 42-43 36.01941907013996 38.0 35.0 40.0 29.5 41.0 44-45 35.78122013136595 38.0 35.0 40.0 28.5 41.0 46-47 35.43915459920072 38.0 34.5 40.0 27.0 41.0 48-49 35.116059789235706 38.0 34.0 40.0 26.5 41.0 50-51 34.862313706336174 38.0 34.0 40.0 26.0 41.0 52-53 34.63183076767357 38.0 34.0 40.0 24.5 41.0 54-55 34.347639312234264 38.0 33.0 40.0 24.0 41.0 56-57 32.35575542891613 37.0 30.5 39.5 15.5 41.0 58-59 27.320730793555477 32.5 17.5 38.5 6.5 40.0 60-61 28.062778731460643 33.0 19.0 38.0 7.0 40.0 62-63 31.187103407376412 34.5 29.5 38.5 8.0 40.0 64-65 31.444451898040242 35.5 30.5 39.0 4.5 40.0 66-67 24.76312980148376 27.0 18.0 35.5 2.0 39.0 68-69 17.30541308809154 13.0 7.0 28.0 2.0 35.5 70-71 16.19302499675627 12.5 7.0 26.5 2.0 33.5 72-73 17.308731372331906 13.5 7.0 28.5 2.0 34.0 74-75 18.300191532881225 17.5 8.5 29.5 2.0 34.5 76-77 19.490228732173925 21.0 8.0 30.0 2.0 34.5 78-79 22.542144027020434 27.5 10.5 33.5 2.0 36.0 80-81 26.206826810663344 31.5 22.5 34.5 2.0 36.0 82-83 27.517111776537952 33.5 26.5 35.0 2.0 36.0 84-85 27.557662979570956 34.0 27.0 35.0 2.0 36.0 86-87 27.312718951735345 34.0 26.5 35.0 2.0 36.0 88-89 26.89089928406986 33.0 26.0 35.0 2.0 35.5 90-91 25.518739075764685 32.5 16.0 35.0 2.0 35.0 92-93 23.055160552717908 31.5 2.0 34.5 2.0 35.0 94-95 15.733770144238967 4.5 2.0 32.0 2.0 35.0 96-97 13.176992651848995 2.0 2.0 30.0 2.0 34.0 98-99 12.868623524272945 2.0 2.0 29.5 2.0 34.0 100-101 12.577371268097803 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3482572218418234 1101 2 0.28958459738364084 1101 3 0.4141395660263605 1101 4 0.28256830234155217 1101 5 0.35155644260732544 1101 6 0.34997617111661583 1101 7 0.43814552721072886 1101 8 0.337998650387739 1101 9 0.31196648777614655 1101 10-11 0.40501368045652697 1101 12-13 0.4316749028656517 1101 14-15 0.5155714312527593 1101 16-17 0.5059738184056215 1101 18-19 0.36559911663555766 1101 20-21 0.419184225173467 1101 22-23 0.48099039969484636 1101 24-25 0.41848499842986087 1101 26-27 0.48060932049919813 1101 28-29 0.5319567424010714 1101 30-31 0.5974629474447894 1101 32-33 0.5934658779805275 1101 34-35 0.40464926820740743 1101 36-37 0.2715269333505361 1101 38-39 0.566611063251024 1101 40-41 0.5205799526686334 1101 42-43 0.612444967175314 1101 44-45 0.394877044658152 1101 46-47 0.35948493630001366 1101 48-49 0.42563989764366994 1101 50-51 0.17674038504770095 1101 52-53 0.14795268108574788 1101 54-55 0.015158273653980814 1101 56-57 -1.8377955578281018 1101 58-59 -9.809774401623088 1101 60-61 -10.177518796652706 1101 62-63 -4.054828745640968 1101 64-65 -5.121993045423974 1101 66-67 -9.843457872683555 1101 68-69 -4.950991934639664 1101 70-71 -4.641691726834356 1101 72-73 -4.636439814949325 1101 74-75 -6.492522073697053 1101 76-77 -8.17374214207484 1101 78-79 -11.104213132452841 1101 80-81 -8.240454990747029 1101 82-83 -4.467514767974034 1101 84-85 -3.614761329870575 1101 86-87 -3.3716735089368477 1101 88-89 -3.610910353695207 1101 90-91 -9.768118572691204 1101 92-93 -16.835750004820376 1101 94-95 -10.921644243637113 1101 96-97 -9.12780585601247 1101 98-99 -9.125467775710595 1101 100-101 -9.079369461236638 1102 1 0.3268133919074998 1102 2 0.3116540754341095 1102 3 0.3339708829983614 1102 4 0.24793193870518593 1102 5 0.3247406804227424 1102 6 0.2647964269064502 1102 7 0.38497822683049776 1102 8 0.38601973576624005 1102 9 0.49717319361783296 1102 10-11 0.4592876175460603 1102 12-13 0.39754095886288354 1102 14-15 0.5401599207100745 1102 16-17 0.46836994699186363 1102 18-19 0.5073732265560551 1102 20-21 0.545903893338 1102 22-23 0.6188244819623918 1102 24-25 0.5271460768951215 1102 26-27 0.6125901362613817 1102 28-29 0.5436651765524658 1102 30-31 0.4633858648315865 1102 32-33 0.5257118164527057 1102 34-35 0.41666223053025675 1102 36-37 0.31205545046080374 1102 38-39 0.42614338264265683 1102 40-41 0.3346005195542219 1102 42-43 0.40809360181064136 1102 44-45 0.590200065743538 1102 46-47 0.6791667544818338 1102 48-49 0.6067665827456352 1102 50-51 0.6899038831465631 1102 52-53 0.415806984576939 1102 54-55 0.32897628264118595 1102 56-57 -2.4062376594527137 1102 58-59 -10.134042808121531 1102 60-61 -10.245688516666533 1102 62-63 -3.9782212447768153 1102 64-65 -5.155701133913432 1102 66-67 -6.651277609980479 1102 68-69 -4.439428160011252 1102 70-71 -4.291259649406081 1102 72-73 -4.630866707448462 1102 74-75 -5.24555197345509 1102 76-77 -7.455157454898899 1102 78-79 -9.970602174969262 1102 80-81 -5.600432522720759 1102 82-83 -3.122542593760418 1102 84-85 -2.6900625742535667 1102 86-87 -2.598961963827964 1102 88-89 -2.712501435617085 1102 90-91 -8.561720370133308 1102 92-93 -13.02779632352069 1102 94-95 -10.606436327978628 1102 96-97 -9.212408344778456 1102 98-99 -9.122847658185535 1102 100-101 -9.203825907831867 1103 1 0.2673126763328604 1103 2 0.18632995123524054 1103 3 0.24357302349988075 1103 4 0.2169454412874785 1103 5 0.1545593600319748 1103 6 0.14094601311764166 1103 7 0.09830882231304372 1103 8 0.18615040591743792 1103 9 0.2576600332668946 1103 10-11 0.3377865286281292 1103 12-13 0.34885475389742027 1103 14-15 0.506759136689169 1103 16-17 0.3032362278704639 1103 18-19 0.36586180847379524 1103 20-21 0.3635599197209345 1103 22-23 0.41661316966072803 1103 24-25 0.41300457978853444 1103 26-27 0.4389714442073753 1103 28-29 0.3946346246266401 1103 30-31 0.43188315809273803 1103 32-33 0.42031389473034864 1103 34-35 0.41141663286988006 1103 36-37 0.2826933696942078 1103 38-39 0.27165741412794375 1103 40-41 0.29207497665085214 1103 42-43 0.12132687914247953 1103 44-45 0.23270183912415376 1103 46-47 0.2610506447188996 1103 48-49 0.17987566120995524 1103 50-51 0.1605650741116591 1103 52-53 0.35073760494903894 1103 54-55 0.3868795867292931 1103 56-57 -2.5479142197165423 1103 58-59 -10.482256236035166 1103 60-61 -10.495238638003102 1103 62-63 -4.284607655081089 1103 64-65 -5.451932855648231 1103 66-67 -6.633489668968718 1103 68-69 -4.344296432054062 1103 70-71 -4.150997686864859 1103 72-73 -4.253448127397437 1103 74-75 -5.082322180660617 1103 76-77 -6.800202318317478 1103 78-79 -9.066587365685457 1103 80-81 -4.991159297656978 1103 82-83 -2.8420637187681947 1103 84-85 -2.334988341162905 1103 86-87 -2.2238498986173383 1103 88-89 -2.122017582714342 1103 90-91 -4.878808292477903 1103 92-93 -9.893938442866094 1103 94-95 -8.434604954697562 1103 96-97 -8.11531668900097 1103 98-99 -8.318931287082306 1103 100-101 -8.37245076005015 1104 1 0.1328693170122044 1104 2 0.20579401137892006 1104 3 0.1698945095073512 1104 4 0.13792060586458632 1104 5 0.18008411476617425 1104 6 0.10161584055513373 1104 7 0.2534850018982482 1104 8 0.18534838814367305 1104 9 0.1798810034232048 1104 10-11 0.18394553821617166 1104 12-13 0.2339491874906301 1104 14-15 0.3118743824001484 1104 16-17 0.2810409250652768 1104 18-19 0.23260234702907923 1104 20-21 0.2041048048925731 1104 22-23 0.26783852483008985 1104 24-25 0.3155368135297607 1104 26-27 0.3447614838839499 1104 28-29 0.26737360227308926 1104 30-31 0.3438841402688908 1104 32-33 0.31858875658574703 1104 34-35 0.25458216154775215 1104 36-37 0.25486168352313854 1104 38-39 0.2598737349461899 1104 40-41 0.07813969176412172 1104 42-43 0.09654223447878252 1104 44-45 0.15305027515471892 1104 46-47 0.03021664361708787 1104 48-49 -0.0644531758155864 1104 50-51 -0.105810208994356 1104 52-53 0.02895492742000272 1104 54-55 -0.037174616595081034 1104 56-57 -3.2752813894354667 1104 58-59 -10.373064611521386 1104 60-61 -10.489995612010212 1104 62-63 -4.379787270891622 1104 64-65 -5.444647918835827 1104 66-67 -6.580883424220438 1104 68-69 -4.2472015031992285 1104 70-71 -4.089107025607117 1104 72-73 -4.417310166428507 1104 74-75 -4.8443844445070745 1104 76-77 -6.693396922748121 1104 78-79 -9.121673744321317 1104 80-81 -4.860112862705979 1104 82-83 -2.733726629237136 1104 84-85 -2.339288491967288 1104 86-87 -2.117706358999186 1104 88-89 -2.052132132833151 1104 90-91 -4.540869791172973 1104 92-93 -7.016113643057521 1104 94-95 -7.984316840420115 1104 96-97 -8.050268039284493 1104 98-99 -8.256310949636632 1104 100-101 -8.264956728230981 1105 1 0.2560820292006767 1105 2 0.27309994517445446 1105 3 0.3462998165052049 1105 4 0.2511265845501427 1105 5 0.22788575075799855 1105 6 0.16547189322609057 1105 7 0.2503988130609045 1105 8 0.2296585781780891 1105 9 0.37441481225822315 1105 10-11 0.3870178028345279 1105 12-13 0.3657325682078678 1105 14-15 0.46724996222956605 1105 16-17 0.5815224536535695 1105 18-19 0.5546986278078379 1105 20-21 0.42764151495293135 1105 22-23 0.5209849826750386 1105 24-25 0.4891157118954226 1105 26-27 0.5291067130724443 1105 28-29 0.6192188730264121 1105 30-31 0.5821867571907617 1105 32-33 0.5472337846004436 1105 34-35 0.5888569383445983 1105 36-37 0.5074501334873887 1105 38-39 0.41947126266992285 1105 40-41 0.3531128824469505 1105 42-43 0.35528539706378837 1105 44-45 0.42501521106174067 1105 46-47 0.4767736206899258 1105 48-49 0.39139458051473497 1105 50-51 0.5346155395882803 1105 52-53 0.5600215225951573 1105 54-55 0.48976874716370844 1105 56-57 1.6909149711477696 1105 58-59 -8.310772435685518 1105 60-61 -9.718418268169291 1105 62-63 -3.3406363157317642 1105 64-65 -4.449887166013575 1105 66-67 -5.98590362472919 1105 68-69 -3.783986380250454 1105 70-71 -3.8437446218297033 1105 72-73 -2.086129891435572 1105 74-75 -4.223270756376328 1105 76-77 -6.282507767844303 1105 78-79 -8.787734603549541 1105 80-81 -4.155733384095239 1105 82-83 -1.6296150359739663 1105 84-85 -1.1680413807231709 1105 86-87 -1.0785076042815795 1105 88-89 -1.189597426197743 1105 90-91 -3.784964467549184 1105 92-93 -5.868329879943815 1105 94-95 -7.896874823361908 1105 96-97 -8.329287566442956 1105 98-99 -8.653443183995783 1105 100-101 -8.508023714115563 1106 1 0.23705163642160443 1106 2 0.2307888716723525 1106 3 0.15360724663472425 1106 4 0.09653381958293039 1106 5 0.12205729484280425 1106 6 0.27407542377165584 1106 7 0.17442650269256887 1106 8 0.1615996730703131 1106 9 0.15064027825733461 1106 10-11 0.23655721002254637 1106 12-13 0.2674343757907849 1106 14-15 0.26170851005176843 1106 16-17 0.336344868621957 1106 18-19 0.36111930806076487 1106 20-21 0.34576282750727927 1106 22-23 0.3127053597053333 1106 24-25 0.3449924718794435 1106 26-27 0.3461826127504182 1106 28-29 0.37151784112927544 1106 30-31 0.42594072376575554 1106 32-33 0.37872122084142035 1106 34-35 0.5026402869565914 1106 36-37 0.4523313125297719 1106 38-39 0.3769709688495553 1106 40-41 0.2587165070150306 1106 42-43 0.2853914074532753 1106 44-45 0.3511373698187654 1106 46-47 0.36750217767932725 1106 48-49 0.4452744197362293 1106 50-51 0.41107629693966885 1106 52-53 0.5793336303449621 1106 54-55 0.368164370415478 1106 56-57 1.6640413374124918 1106 58-59 -8.624926820660718 1106 60-61 -9.812945569958071 1106 62-63 -3.6917761037109855 1106 64-65 -5.105152544571737 1106 66-67 -6.794334036312765 1106 68-69 -3.9668795706695583 1106 70-71 -4.064864665080062 1106 72-73 -4.500518745066017 1106 74-75 -5.183846644301486 1106 76-77 -6.809746796591689 1106 78-79 -9.378868632649512 1106 80-81 -4.850730328363394 1106 82-83 -2.273360775578599 1106 84-85 -1.7642726056015299 1106 86-87 -1.6041531863984986 1106 88-89 -1.6085077052095649 1106 90-91 -4.075199993957757 1106 92-93 -6.553219521012853 1106 94-95 -7.341426923589912 1106 96-97 -7.384069786784727 1106 98-99 -6.837549852542901 1106 100-101 -7.192681707204908 1107 1 0.05914911130621192 1107 2 -0.036398656610749924 1107 3 0.052531131149159194 1107 4 0.05947118948066077 1107 5 0.07072278493240702 1107 6 0.035161494909317526 1107 7 0.07040079900897922 1107 8 0.15165442915683514 1107 9 0.05387541265863405 1107 10-11 0.016237271566573952 1107 12-13 0.03502759689677504 1107 14-15 0.09036834352085776 1107 16-17 0.0656751247537315 1107 18-19 0.0385966815787242 1107 20-21 0.11730391719866162 1107 22-23 0.15500964063573974 1107 24-25 0.19015538255008835 1107 26-27 0.13583733263456566 1107 28-29 0.1353718098142167 1107 30-31 0.18865906931738863 1107 32-33 0.18336213857153183 1107 34-35 0.17467237130811242 1107 36-37 0.11219849508022861 1107 38-39 -0.01830281158306235 1107 40-41 0.16745055534519793 1107 42-43 -0.07558583030579769 1107 44-45 -0.08706586124286986 1107 46-47 0.04370409640500128 1107 48-49 -0.025828621262952822 1107 50-51 -0.07553312472744977 1107 52-53 -0.10089964868480905 1107 54-55 -0.18982436159646454 1107 56-57 0.9629489308598593 1107 58-59 -8.754434242146825 1107 60-61 -10.056916743819958 1107 62-63 -3.949002323278364 1107 64-65 -5.511965152047527 1107 66-67 -6.955361957391599 1107 68-69 -3.8484887660718563 1107 70-71 -4.021881815953446 1107 72-73 -2.601521324484967 1107 74-75 -4.6902463184801455 1107 76-77 -6.7138781659463795 1107 78-79 -7.485676262299254 1107 80-81 -2.456885636536075 1107 82-83 -1.2853358411953977 1107 84-85 -1.0766599257209961 1107 86-87 -1.0562612957296587 1107 88-89 -0.8624809154548352 1107 90-91 -0.3357319425503924 1107 92-93 -3.2515752545633987 1107 94-95 -5.990755822132767 1107 96-97 -6.728112234065025 1107 98-99 -6.249478533871528 1107 100-101 -6.936868737705406 1108 1 -0.08650404475420004 1108 2 -0.08779900892929504 1108 3 -0.09504591954978281 1108 4 -0.01898264590659693 1108 5 0.058030648570955634 1108 6 -0.11014585999340198 1108 7 -0.10519342215696525 1108 8 -0.06464213852147793 1108 9 -0.0591844814939364 1108 10-11 -0.03624857368155432 1108 12-13 -0.020501646554841102 1108 14-15 -0.16713630090823273 1108 16-17 -0.16289342929754724 1108 18-19 -0.1595339357127301 1108 20-21 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2316 82-83 2.1694030191939078 2316 84-85 1.7733547157813412 2316 86-87 1.5543142836418937 2316 88-89 1.7667790947592792 2316 90-91 2.838796512145432 2316 92-93 4.9208401867905565 2316 94-95 11.94449411879306 2316 96-97 13.669078058460123 2316 98-99 13.38589152454203 2316 100-101 12.305187850271919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 61.0 5 129.0 6 288.0 7 752.0 8 1569.0 9 2753.0 10 4809.0 11 7690.0 12 11382.0 13 15729.0 14 19378.0 15 26154.0 16 35987.0 17 47851.0 18 51112.0 19 49129.0 20 50307.0 21 50178.0 22 49695.0 23 42571.0 24 43172.0 25 51351.0 26 72401.0 27 106973.0 28 151995.0 29 206169.0 30 256486.0 31 281221.0 32 282369.0 33 259123.0 34 231199.0 35 207196.0 36 169157.0 37 111333.0 38 43321.0 39 3126.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48270041964732 20.305976984627428 14.400337837837837 22.81098475788741 2 15.169929913867394 22.072970693446116 39.74949973657832 23.007599656108173 3 18.453208465786023 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29.051933849697605 21.78659134975371 48-49 21.665918859747183 27.664412817983003 28.94652055556585 21.723147766703963 50-51 21.412172027771337 27.80391135424367 28.955133745954438 21.828782872030562 52-53 21.81205235911083 27.936650017745855 28.574225947567456 21.677071675575856 54-55 21.374461486375786 27.61545793288269 28.83361875036966 22.176461830371863 56-57 20.87160952800197 27.965411149424778 29.417786336785888 21.745192985787366 58-59 20.6534038573393 27.257922738679163 30.353174304735735 21.735499099245796 60-61 21.60495702766794 27.686342602866276 29.175250094397793 21.533450275067988 62-63 21.687223144813373 27.642532972962325 28.87053783572911 21.799706046495196 64-65 21.091256067157463 27.699121017719918 29.12080858820623 22.08881432691639 66-67 21.216492069403277 27.593018555256677 29.145479414505026 22.04500996083502 68-69 21.523581991069907 27.08587876432548 29.535189156394075 21.855350088210542 70-71 21.388977733952718 27.302672009475152 29.392523233954115 21.915827022618014 72-73 20.667845886116826 27.243804347655555 29.957502427847476 22.130847338380143 74-75 21.010090650582423 27.637773258857774 29.29379305278994 22.058343037769863 76-77 19.32497467311834 27.9604985026014 30.15024269550002 22.56428412878024 78-79 20.06899531866454 27.97272343473684 29.41450824894468 22.54377299765393 80-81 21.02793130540928 28.212956493607116 28.396617604280227 22.362494596703378 82-83 21.46289147746282 28.077075269985922 28.073831532861092 22.386201719690163 84-85 21.33093783589031 27.881538700083162 27.943882998551867 22.843640465474657 86-87 21.311042205662723 27.83216847348165 28.189370649607643 22.667418671247987 88-89 21.45534982656211 27.7398559000726 27.917174384688217 22.887619888677076 90-91 20.658677384718604 27.933063134825897 28.556850897513144 22.851408582942355 92-93 19.794657690363575 28.05096989932345 29.310351587972004 22.844020822340973 94-95 19.141331942843234 27.906755166048942 29.40365124914574 23.548261641962082 96-97 19.282421574498006 28.050984361348974 28.937993394099088 23.728600670053936 98-99 19.337617987280364 27.910318473092616 28.776152663184522 23.975910876442498 100-101 19.718254330807326 27.82034072565888 28.651096712750068 23.810308230783722 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 863.0 1 642.0 2 455.5 3 529.0 4 595.0 5 687.5 6 917.0 7 1304.0 8 1775.5 9 2259.0 10 2551.0 11 2770.0 12 3083.0 13 3549.0 14 4059.5 15 4391.5 16 4747.5 17 5216.5 18 5693.0 19 6189.5 20 6922.0 21 7933.5 22 9189.0 23 10845.0 24 13075.5 25 16199.5 26 20405.0 27 25015.0 28 29827.0 29 35394.5 30 41834.0 31 49305.5 32 57648.0 33 66326.5 34 75233.0 35 83468.5 36 90389.5 37 95620.0 38 98524.0 39 99902.5 40 101082.0 41 102233.5 42 104175.0 43 105748.0 44 106827.5 45 108553.0 46 110326.0 47 112453.5 48 114335.5 49 111947.5 50 105263.5 51 98852.0 52 92168.0 53 85565.5 54 79392.0 55 71932.0 56 64028.5 57 56288.0 58 48607.0 59 41262.0 60 34175.0 61 27478.5 62 21371.0 63 16166.0 64 11946.5 65 8611.0 66 6079.5 67 4225.0 68 2794.0 69 1769.5 70 1169.0 71 782.0 72 558.5 73 393.0 74 265.0 75 200.0 76 125.5 77 48.5 78 17.5 79 8.0 80 4.0 81 2.0 82 2.0 83 1.5 84 1.0 85 1.5 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024387410661578145 2 0.006555390331037022 3 3.0569177709499065E-4 4 0.0 5 0.14635842972247942 6 0.0 7 0.0 8 0.0014265616264432898 9 0.0 10-11 0.0 12-13 0.0 14-15 6.113835541899813E-4 16-17 0.0 18-19 1.8681164155804985E-4 20-21 0.0 22-23 2.887089005897134E-4 24-25 0.0 26-27 2.3776027107388163E-4 28-29 0.00315881502998157 30-31 0.012482414231378787 32-33 0.002632345858317975 34-35 0.004296667755835147 36-37 0.11040568016080746 38-39 0.10994714249516496 40-41 0.12190308755488018 42-43 0.12460336491921925 44-45 0.12013686839833133 46-47 0.11290216300708321 48-49 0.11278328287154628 50-51 0.11100008083849217 52-53 0.08865061535754729 54-55 0.07635501276772656 56-57 0.5425349728375873 58-59 1.5440831318598085 60-61 0.5111166513028244 62-63 0.004721239668467078 64-65 0.11587416639550674 66-67 1.862206374556662 68-69 2.812568143791977 70-71 2.7250723640367887 72-73 2.576659006257171 74-75 1.9701835033772148 76-77 1.379230349623082 78-79 0.9215757935588705 80-81 0.05074483499776845 82-83 2.3776027107388163E-4 84-85 2.547431475791589E-4 86-87 3.736232831160997E-4 88-89 1.5284588854749532E-4 90-91 0.24338160319712837 92-93 0.38158825219707476 94-95 1.9655811438442847 96-97 2.379131169624291 98-99 1.3843421954511705 100-101 0.9245647798237993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944142.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.682569082628575 #Duplication Level Percentage of deduplicated Percentage of total 1 70.82145287039539 30.936630075431708 2 14.46593695047639 12.638185803682687 3 5.805995961492223 7.608624590440397 4 2.802944369519255 4.897592442251586 5 1.6198348933399724 3.5379274815387816 6 0.9631953922096991 2.5244909556161828 7 0.6609898779268393 2.021161520382017 8 0.4791476667201385 1.6744320841826192 9 0.338492162972953 1.330758656369495 >10 1.764631856592632 14.021486803640276 >50 0.15821727324686993 4.831212133082065 >100 0.10910930365697578 8.924256259582247 >500 0.007869717570006002 2.4021317970881118 >1k 0.0018700318977928215 1.3198365525265692 >5k 1.5583599148273513E-4 0.4528907643169541 >10k+ 1.5583599148273513E-4 0.8783820798683025 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14650 0.4975982816046237 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11157 0.37895590633875675 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7846 0.26649529812081074 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5460 0.18545301143762769 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3039 0.10322192339907517 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.3965753010554516E-5 0.0 0.0 0.0 0.0 7 3.3965753010554516E-5 0.0 0.0 0.0 0.0 8 3.3965753010554516E-5 0.0 0.0 0.0 0.0 9 3.3965753010554516E-5 0.0 0.0 0.0 0.0 10-11 3.3965753010554516E-5 0.0 0.0 1.6982876505277258E-5 0.0 12-13 3.3965753010554516E-5 0.0 0.0 6.793150602110903E-5 0.0 14-15 3.3965753010554516E-5 0.0 0.0 6.793150602110903E-5 0.0 16-17 3.3965753010554516E-5 0.0 0.0 1.188801355369408E-4 0.0 18-19 3.3965753010554516E-5 0.0 0.0 3.0569177709499065E-4 0.0 20-21 3.3965753010554516E-5 0.0 0.0 5.26469171663595E-4 0.0 22-23 3.3965753010554516E-5 0.0 0.0 9.510410842955266E-4 0.0 24-25 3.3965753010554516E-5 0.0 0.0 0.001511476008969676 0.0 26-27 3.3965753010554516E-5 0.0 0.0 0.002275705451707153 0.0 28-29 3.3965753010554516E-5 0.0 0.0 0.0048231369274987414 0.0 30-31 3.3965753010554516E-5 0.0 0.0 0.012974917650031826 0.0 32-33 3.3965753010554516E-5 0.0 0.0 0.029770982513751036 0.0 34-35 3.3965753010554516E-5 0.0 0.0 0.055432108913224976 0.0 36-37 3.3965753010554516E-5 0.0 0.0 0.09440781049283628 0.0 38-39 3.3965753010554516E-5 0.0 0.0 0.1496700906410085 0.0 40-41 3.3965753010554516E-5 0.0 0.0 0.21201423029188132 0.0 42-43 3.3965753010554516E-5 0.0 0.0 0.2773643390841882 0.0 44-45 3.3965753010554516E-5 0.0 0.0 0.35701402989393854 0.0 46-47 3.3965753010554516E-5 0.0 0.0 0.44683646373034996 0.0 48-49 3.3965753010554516E-5 3.3965753010554516E-5 0.0 0.5315640346151782 0.0 50-51 3.3965753010554516E-5 8.491438252638629E-5 0.0 0.6120798521266977 0.0 52-53 3.3965753010554516E-5 1.3586301204221806E-4 0.0 0.6920692004665536 0.0 54-55 3.3965753010554516E-5 1.3586301204221806E-4 0.0 0.7769835829929399 0.0 56-57 3.3965753010554516E-5 1.3586301204221806E-4 0.0 0.8504854725077798 0.0 58-59 3.3965753010554516E-5 1.3586301204221806E-4 0.0 0.9010434958639904 0.0 60-61 3.3965753010554516E-5 1.3586301204221806E-4 0.0 0.9430081837085303 0.0 62-63 6.793150602110903E-5 1.3586301204221806E-4 0.0 0.9801327517490664 0.0 64-65 6.793150602110903E-5 1.3586301204221806E-4 0.0 1.0180555149853505 0.0 66-67 6.793150602110903E-5 1.5284588854749532E-4 3.3965753010554516E-5 1.0505437577399461 0.0 68-69 6.793150602110903E-5 1.6982876505277259E-4 3.3965753010554516E-5 1.0952257058253305 0.0 70-71 6.793150602110903E-5 1.6982876505277259E-4 3.3965753010554516E-5 1.1666047357770108 0.0 72-73 6.793150602110903E-5 1.6982876505277259E-4 3.3965753010554516E-5 1.264069464040797 0.0 74-75 6.793150602110903E-5 2.037945180633271E-4 3.3965753010554516E-5 1.3946508014898737 0.0 76-77 6.793150602110903E-5 2.037945180633271E-4 3.3965753010554516E-5 1.5993623948844857 0.0 78-79 6.793150602110903E-5 2.037945180633271E-4 3.3965753010554516E-5 1.9063958192233934 0.0 80-81 6.793150602110903E-5 2.037945180633271E-4 3.3965753010554516E-5 2.285232845426613 0.0 82-83 6.793150602110903E-5 2.037945180633271E-4 3.3965753010554516E-5 2.6613865771419993 0.0 84-85 6.793150602110903E-5 3.2267465360026794E-4 3.3965753010554516E-5 2.9450345805331404 0.0 86-87 6.793150602110903E-5 4.075890361266542E-4 3.3965753010554516E-5 3.161634187481446 0.0 88-89 6.793150602110903E-5 4.075890361266542E-4 3.3965753010554516E-5 3.36767044524347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 19820 0.0 26.951822 1 GGTATCA 13745 0.0 26.49192 1 GTACATG 14575 0.0 26.437687 1 TACATGG 14630 0.0 25.818293 2 ACATGGG 14235 0.0 24.812986 3 ATCAACG 23450 0.0 22.513243 3 TCAACGC 23545 0.0 22.402403 4 CAACGCA 23725 0.0 22.272139 5 AACGCAG 23810 0.0 22.258589 6 CATGGGA 9635 0.0 21.702337 4 TATCAAC 24450 0.0 21.61282 2 GAGTACT 11160 0.0 21.300152 12-13 CATGGGG 4775 0.0 20.416075 4 AGTACTT 11820 0.0 20.309723 12-13 ACGCAGA 26340 0.0 19.906376 7 CGCAGAG 26175 0.0 19.74441 8 TAGGACG 410 0.0 19.527216 4 GTACTTT 12440 0.0 19.1841 14-15 AGAGTAC 20655 0.0 19.044365 10-11 ATGGGAG 4185 0.0 18.680475 5 >>END_MODULE