FastQCFastQC Report
Fri 27 May 2016
SRR939668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876529
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59260.20601217648075162No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39200.13627535130012594No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112900.032.5248451
GTATCAA186100.031.1921831
TACATGG139600.027.7830682
ACATGGG136150.027.1001073
CATGGGA94900.025.2856774
TATCAAC239350.024.0598412
GGTATCA137650.023.7532731
ATCAACG250200.022.9417513
TCAACGC252300.022.785074
GAGTACT102050.022.73051512-13
CAACGCA253150.022.6146165
AACGCAG257650.022.2196376
ACGCAGA264900.021.3086327
AGTACTT112500.020.43032312-13
ATGGGAG40750.020.2682765
GTACTTT115200.020.15966214-15
CGCAGAG276900.020.1462068
ATGGGAT42450.019.5677685
CATGGGG43950.019.5609324
CGCGGAA3800.019.26782682-83
Produced by FastQC (version 0.11.5)