##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939668_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2876529 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.270058462820991 2.0 2.0 31.0 2.0 33.0 2 22.57372826764479 16.0 16.0 31.0 16.0 34.0 3 27.050081191602796 28.0 25.0 31.0 16.0 34.0 4 32.29942058640813 35.0 32.0 35.0 25.0 37.0 5 32.91790939705457 35.0 33.0 35.0 26.0 37.0 6 33.309444820476344 35.0 33.0 37.0 28.0 37.0 7 33.48611399363608 35.0 33.0 37.0 28.0 37.0 8 33.41385120747957 35.0 33.0 37.0 27.0 37.0 9 34.77628628113953 37.0 34.0 39.0 27.0 39.0 10-11 34.613904639932365 37.0 34.0 39.0 27.0 39.0 12-13 34.6235384729304 37.0 34.0 39.0 27.0 39.0 14-15 35.295561247600844 38.0 33.5 40.0 27.0 41.0 16-17 34.922606377338795 38.0 33.0 40.0 25.0 41.0 18-19 35.20348638932547 38.0 33.5 40.0 26.0 41.0 20-21 35.22152027669459 38.0 34.0 40.0 26.0 41.0 22-23 35.04237728873931 38.0 34.0 40.0 25.5 41.0 24-25 34.840959538388105 38.0 33.0 40.0 25.0 41.0 26-27 34.92409445550523 38.0 34.0 40.0 25.0 41.0 28-29 34.674450527006684 38.0 33.0 40.0 24.0 41.0 30-31 34.525223281253204 38.0 33.0 40.0 24.0 41.0 32-33 34.19651983345205 38.0 33.0 40.0 24.0 41.0 34-35 33.916750708927324 38.0 33.0 40.0 22.0 41.0 36-37 33.617694624319796 38.0 32.0 40.0 19.0 41.0 38-39 33.41276726221081 37.0 31.5 40.0 18.5 41.0 40-41 33.02742993378478 37.0 31.0 40.0 17.0 41.0 42-43 32.76053396298108 37.0 31.0 40.0 15.0 41.0 44-45 32.39183091844372 37.0 30.5 40.0 14.0 41.0 46-47 32.01534523031056 37.0 30.0 39.5 9.5 41.0 48-49 31.623563850738165 36.5 30.0 39.0 8.5 40.0 50-51 30.957164693976665 35.5 29.0 38.5 8.0 40.0 52-53 31.00599976568983 36.0 29.0 39.0 7.0 40.0 54-55 31.13679229376794 36.0 29.0 39.0 4.0 40.0 56-57 30.858070438365125 36.0 28.5 39.0 2.0 40.0 58-59 30.46447020002232 35.0 28.0 39.0 2.0 40.0 60-61 29.895559370338347 35.0 27.5 38.0 2.0 40.0 62-63 29.518377356876986 34.0 27.0 38.0 2.0 40.0 64-65 28.965190164952276 34.0 26.0 38.0 2.0 40.0 66-67 28.374039684633807 34.0 26.0 37.5 2.0 39.0 68-69 28.02704700700045 33.5 25.5 37.0 2.0 39.0 70-71 27.52276111243794 33.0 24.5 36.0 2.0 39.0 72-73 26.944737911559386 32.0 22.5 36.0 2.0 38.5 74-75 26.38252856133208 32.0 22.0 35.0 2.0 38.0 76-77 25.74980001939838 31.5 20.0 35.0 2.0 37.0 78-79 25.091216879788107 31.0 18.5 34.5 2.0 36.5 80-81 24.3593984277579 30.5 14.5 34.0 2.0 36.0 82-83 23.666915056305704 30.0 7.5 34.0 2.0 35.5 84-85 22.973181914731263 29.5 4.5 34.0 2.0 35.0 86-87 22.295360658627118 29.0 2.0 34.0 2.0 35.0 88-89 21.473904486970234 29.0 2.0 33.5 2.0 35.0 90-91 20.512627197570403 27.0 2.0 33.0 2.0 34.0 92-93 19.455785775147756 25.5 2.0 32.0 2.0 34.0 94-95 18.46418461277463 25.0 2.0 32.0 2.0 34.0 96-97 17.559654013569826 23.0 2.0 32.0 2.0 34.0 98-99 16.50829506672799 18.0 2.0 31.0 2.0 34.0 100-101 14.921718675528737 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.932153979921548 1101 2 8.056248856845258 1101 3 3.8931705282330284 1101 4 2.5034052515097116 1101 5 1.6482840996027974 1101 6 1.3958683029485854 1101 7 1.0794819071741983 1101 8 1.0692877670410539 1101 9 1.2284338807621467 1101 10-11 1.1518483707982128 1101 12-13 0.8027581964786066 1101 14-15 0.95859704621963 1101 16-17 1.0480882114309082 1101 18-19 1.1626493793762336 1101 20-21 1.1146442752902672 1101 22-23 1.2527210500434904 1101 24-25 1.2877183746629015 1101 26-27 1.3377006229493702 1101 28-29 1.3339525506023904 1101 30-31 1.3934736485111472 1101 32-33 1.3076924189629935 1101 34-35 1.3907073967649737 1101 36-37 1.4176091708521739 1101 38-39 1.132119333288415 1101 40-41 1.2209704762680715 1101 42-43 1.14128619488352 1101 44-45 0.9464451409443058 1101 46-47 0.6762764827785475 1101 48-49 0.8810672581608436 1101 50-51 0.8638435973303835 1101 52-53 0.9626561493745243 1101 54-55 0.8800095097164409 1101 56-57 0.6899003348883959 1101 58-59 0.8636336831848865 1101 60-61 1.005852105679974 1101 62-63 0.7354155866255887 1101 64-65 0.7194334927265729 1101 66-67 0.44929170172857624 1101 68-69 0.46983960241071543 1101 70-71 0.19668760199379065 1101 72-73 0.18852121504922437 1101 74-75 0.006382263400141142 1101 76-77 -0.010520698752443991 1101 78-79 -0.11784402840723729 1101 80-81 -0.12864999821572098 1101 82-83 -0.035031629183688295 1101 84-85 -0.1645471509945402 1101 86-87 -0.27610857927651367 1101 88-89 -0.08693185346560739 1101 90-91 -0.02273056822401287 1101 92-93 -0.03457047233042232 1101 94-95 -0.08765667524529519 1101 96-97 -0.9004604872965949 1101 98-99 -1.1023826626720457 1101 100-101 -1.3596049147984157 1102 1 14.652360929197698 1102 2 7.948681788980519 1102 3 3.6516719477544477 1102 4 2.1234221696154165 1102 5 1.5524768765141985 1102 6 1.310576344237525 1102 7 0.96963346560117 1102 8 0.944449617236323 1102 9 0.8657575830567978 1102 10-11 1.007145489674329 1102 12-13 0.7491842347476094 1102 14-15 0.7826456526894461 1102 16-17 0.9919822462039463 1102 18-19 0.8751200083260926 1102 20-21 0.8372137613873392 1102 22-23 0.8118275512802171 1102 24-25 0.7470312587000905 1102 26-27 0.7887958013368461 1102 28-29 0.9142759463337384 1102 30-31 0.9131815279498952 1102 32-33 0.9336384095528345 1102 34-35 0.8812374942368706 1102 36-37 0.8363695364051225 1102 38-39 0.7945611148211142 1102 40-41 0.759132848192202 1102 42-43 0.8426875795595379 1102 44-45 0.7674816676771954 1102 46-47 0.6299212777714729 1102 48-49 0.5683534016895955 1102 50-51 0.4284070683125485 1102 52-53 0.5901228177548958 1102 54-55 0.4863650812666016 1102 56-57 0.5113270872023783 1102 58-59 0.53013635752135 1102 60-61 0.44376898894028116 1102 62-63 0.07140576231873652 1102 64-65 0.1540223313466953 1102 66-67 0.02762314998316384 1102 68-69 -0.07950435686396418 1102 70-71 -0.13833135352025394 1102 72-73 0.024504726322565062 1102 74-75 -0.06636535311771752 1102 76-77 -0.34549693057524067 1102 78-79 -0.27846130601078656 1102 80-81 -0.1728748500275863 1102 82-83 -0.22965815509780896 1102 84-85 -0.47720959622729353 1102 86-87 -0.48482975804100903 1102 88-89 -0.5822437911551823 1102 90-91 -0.2796892935680404 1102 92-93 0.037629590695949844 1102 94-95 -0.16151986803553342 1102 96-97 -0.889184407993806 1102 98-99 -1.167165583477793 1102 100-101 -1.1186883999853148 1103 1 14.769683707610284 1103 2 7.872585278476869 1103 3 3.731332849866895 1103 4 2.2328177703889054 1103 5 1.5678157546601668 1103 6 1.1497209268750055 1103 7 0.9431720009502627 1103 8 0.9429535432859595 1103 9 1.0910137651200245 1103 10-11 0.9419092491598349 1103 12-13 0.7207080144493432 1103 14-15 0.8807552341549822 1103 16-17 0.9913601028445314 1103 18-19 0.870772787324654 1103 20-21 0.7829769605318049 1103 22-23 0.9312900633659993 1103 24-25 1.0261749440594414 1103 26-27 1.1026824622902325 1103 28-29 1.1057647138764395 1103 30-31 1.082568870102186 1103 32-33 1.090334759645124 1103 34-35 1.1252930265974825 1103 36-37 1.2374597293382337 1103 38-39 1.0551654774240333 1103 40-41 0.9779547220019396 1103 42-43 0.9447992046218019 1103 44-45 0.9855381083030181 1103 46-47 1.013913286502543 1103 48-49 0.8261478187884244 1103 50-51 0.8907873091493244 1103 52-53 0.9801229276256862 1103 54-55 0.7083553540391527 1103 56-57 0.9603832055405483 1103 58-59 0.9721872972828578 1103 60-61 0.915669076104443 1103 62-63 0.7381594100290272 1103 64-65 0.5233269041690569 1103 66-67 0.5479996039399779 1103 68-69 0.355085440534296 1103 70-71 0.2913449305030653 1103 72-73 0.30059812382043916 1103 74-75 0.2762941792721918 1103 76-77 0.10534367254215837 1103 78-79 0.008072105697042531 1103 80-81 0.012350824579076658 1103 82-83 0.07217613777377707 1103 84-85 0.00447906290956368 1103 86-87 -0.030416980800378468 1103 88-89 -0.06780081775856672 1103 90-91 -0.07882396952244974 1103 92-93 0.08280388239557723 1103 94-95 0.03709218918517365 1103 96-97 -0.601614090686958 1103 98-99 -0.8263550849573864 1103 100-101 -0.6270202683747552 1104 1 14.866524172991745 1104 2 8.175291034202225 1104 3 3.9304004484687276 1104 4 2.322283079914996 1104 5 1.7040709764809847 1104 6 1.344502502905783 1104 7 1.0059054509753054 1104 8 0.977913766865143 1104 9 1.150147888653386 1104 10-11 1.0270041144581725 1104 12-13 0.7221375212004162 1104 14-15 0.7632051539627298 1104 16-17 0.9898556656289372 1104 18-19 0.9687292170911661 1104 20-21 0.8904662105078316 1104 22-23 0.8878876741165556 1104 24-25 1.0132897348669019 1104 26-27 1.0178966435300367 1104 28-29 1.0938739152042771 1104 30-31 0.9955427969647275 1104 32-33 0.9887332125474941 1104 34-35 0.9730581339112305 1104 36-37 1.1077326160993195 1104 38-39 1.0191508989528515 1104 40-41 1.226666417548472 1104 42-43 0.9590318300787075 1104 44-45 0.810673549280466 1104 46-47 0.7718495882811567 1104 48-49 0.6010330586191905 1104 50-51 0.8158035607345191 1104 52-53 0.8147327430503033 1104 54-55 0.825401401973334 1104 56-57 0.7199295132336765 1104 58-59 0.6575807521567363 1104 60-61 0.6139176014729024 1104 62-63 0.5008826029060351 1104 64-65 0.5707434016593851 1104 66-67 0.4960594710499642 1104 68-69 0.4343696980766296 1104 70-71 0.37964199083107886 1104 72-73 0.4725721985457625 1104 74-75 0.36601558447482674 1104 76-77 0.15040535530055976 1104 78-79 0.06514897169872924 1104 80-81 0.018495122670447728 1104 82-83 -0.06114514008037375 1104 84-85 0.008828526607249643 1104 86-87 -0.19162071706645278 1104 88-89 -0.22334563883127956 1104 90-91 -0.04199897644935646 1104 92-93 0.12698721359755538 1104 94-95 0.023851976102914563 1104 96-97 -0.2696754630667222 1104 98-99 -0.5512055903550426 1104 100-101 -0.5447199763790618 1105 1 14.747128180997912 1105 2 7.930839047588233 1105 3 3.7977080616246788 1105 4 2.3571153813358094 1105 5 1.715228632723118 1105 6 1.2026893900273805 1105 7 1.0451654922779383 1105 8 1.1567903639776134 1105 9 1.2137595193321502 1105 10-11 0.8902287207754327 1105 12-13 0.8268125084988611 1105 14-15 0.9055894827426698 1105 16-17 1.195810139525591 1105 18-19 1.098992303063838 1105 20-21 1.0690030288897034 1105 22-23 0.8985694985840667 1105 24-25 0.8945897421187112 1105 26-27 0.8319436003952916 1105 28-29 1.1228480959406468 1105 30-31 1.0368011683918255 1105 32-33 0.6599912202953888 1105 34-35 0.8729259245426348 1105 36-37 0.8992168715118751 1105 38-39 0.765307169076344 1105 40-41 0.8132030678103774 1105 42-43 0.9069068546597236 1105 44-45 0.8958824982279552 1105 46-47 0.5846304188591276 1105 48-49 0.7344808420162146 1105 50-51 0.6334192014665057 1105 52-53 0.822058810203778 1105 54-55 0.6923556800929838 1105 56-57 0.6040620806194035 1105 58-59 0.6009197497392478 1105 60-61 0.5250290138352511 1105 62-63 0.2905089911207419 1105 64-65 0.40452447418202553 1105 66-67 0.3644948708433873 1105 68-69 0.25499832302732983 1105 70-71 0.28592893720843193 1105 72-73 0.2428558073497591 1105 74-75 0.23246430494467774 1105 76-77 0.26960491696912214 1105 78-79 0.09245082696354956 1105 80-81 0.04089160965998673 1105 82-83 -0.020813660639898757 1105 84-85 -0.029759579253450852 1105 86-87 0.10094461289402545 1105 88-89 -0.017607466952998863 1105 90-91 0.07049173829787847 1105 92-93 0.2748081653961236 1105 94-95 0.2719821858891933 1105 96-97 -0.022784375842295645 1105 98-99 -0.27338097345406354 1105 100-101 -0.084039197217054 1106 1 14.718155153999643 1106 2 7.801609008302254 1106 3 3.5071443207038726 1106 4 2.3053497779363923 1106 5 1.673580355716247 1106 6 1.1629927769343524 1106 7 0.836558129413973 1106 8 0.8743016624538882 1106 9 0.8870944618060932 1106 10-11 0.9576752760308977 1106 12-13 0.6495719508357851 1106 14-15 0.8007609063223597 1106 16-17 0.9275685410041419 1106 18-19 0.8186150539884807 1106 20-21 0.676549644226526 1106 22-23 0.603688417415853 1106 24-25 0.9144622293432576 1106 26-27 0.6778523385952298 1106 28-29 0.8036790486027172 1106 30-31 0.674530716004007 1106 32-33 0.684791337582574 1106 34-35 0.6802529749661019 1106 36-37 0.7750509415208242 1106 38-39 0.899859135967553 1106 40-41 0.9400998895107691 1106 42-43 0.7420148900079511 1106 44-45 0.7243491251889367 1106 46-47 0.7400784127560058 1106 48-49 0.7691767384026598 1106 50-51 0.7200786124774687 1106 52-53 0.7135748123665309 1106 54-55 0.809876934037586 1106 56-57 0.7880553649262296 1106 58-59 0.8584073729354458 1106 60-61 0.7983519313233742 1106 62-63 0.7593460878534728 1106 64-65 0.5972190992312036 1106 66-67 0.6100556469080907 1106 68-69 0.5875522470285475 1106 70-71 0.7654566166780796 1106 72-73 0.6601115966615545 1106 74-75 0.5769585824020531 1106 76-77 0.5144101013064919 1106 78-79 0.5639057543975561 1106 80-81 0.5457375482428084 1106 82-83 0.4988885815407009 1106 84-85 0.5134586990735635 1106 86-87 0.5481751845906082 1106 88-89 0.6369267805116223 1106 90-91 0.8729855940093785 1106 92-93 1.3299666882573185 1106 94-95 0.9830707913001788 1106 96-97 0.6274037759740203 1106 98-99 0.3442990887273112 1106 100-101 0.24849098179403128 1107 1 14.81166979133863 1107 2 8.051860835337479 1107 3 3.688163481045496 1107 4 2.0705823053945664 1107 5 1.4290275120045735 1107 6 1.1351959712700435 1107 7 0.8169603135193029 1107 8 0.8156839780077689 1107 9 0.598452118937999 1107 10-11 0.6462057363586808 1107 12-13 0.6059435615919782 1107 14-15 0.5941409064426821 1107 16-17 0.8759288504849607 1107 18-19 0.5930879361703774 1107 20-21 0.6140568960716095 1107 22-23 0.6461323386292293 1107 24-25 0.7620054546373467 1107 26-27 0.6649152618343948 1107 28-29 0.7077730213418008 1107 30-31 0.6030672743277563 1107 32-33 0.7516322920500045 1107 34-35 0.6952272293045283 1107 36-37 0.5854772898483205 1107 38-39 0.6806810890967796 1107 40-41 1.1064991152930403 1107 42-43 0.7976110797703626 1107 44-45 0.6541935940616526 1107 46-47 0.6911731512899308 1107 48-49 0.6885376177173477 1107 50-51 0.8385672076350019 1107 52-53 0.5398923705553393 1107 54-55 0.5955167981713174 1107 56-57 0.6130398728364348 1107 58-59 0.7432873941129827 1107 60-61 0.8465847587393398 1107 62-63 0.6400520544352517 1107 64-65 0.5580133685761197 1107 66-67 0.6816352255404361 1107 68-69 0.8293666866016629 1107 70-71 0.8499677928994167 1107 72-73 0.8494523298134204 1107 74-75 0.8042918931024658 1107 76-77 0.601497777510211 1107 78-79 0.8189970868204135 1107 80-81 0.7664328959746882 1107 82-83 0.7794140147820379 1107 84-85 0.7817367865976372 1107 86-87 0.6032435474656275 1107 88-89 0.6787893723360945 1107 90-91 0.9474606463730737 1107 92-93 1.1267991038114644 1107 94-95 1.108959061746944 1107 96-97 0.6002767184912763 1107 98-99 0.2351855714599722 1107 100-101 0.4266514110860715 1108 1 14.254127528659826 1108 2 7.406542239758803 1108 3 3.1348474862470574 1108 4 -0.22818232399424687 1108 5 0.13741502825944707 1108 6 0.30067061366276704 1108 7 0.367675528083673 1108 8 0.5003393746267548 1108 9 0.47979152075854614 1108 10-11 0.46545151009080143 1108 12-13 0.09082652648144318 1108 14-15 0.1165726359615391 1108 16-17 0.3263514772729934 1108 18-19 0.24734671380367246 1108 20-21 0.1644470131067166 1108 22-23 -0.5047129149468503 1108 24-25 0.07783770431224468 1108 26-27 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2.1071489371777616 2311 96-97 1.7300048895676774 2311 98-99 1.74938102836758 2311 100-101 1.6169255331621013 2312 1 -14.168824356905844 2312 2 -7.247684548758194 2312 3 -3.319347203018978 2312 4 -1.3614749141735203 2312 5 -1.802176003654374 2312 6 -0.639589035836643 2312 7 -0.127479547542805 2312 8 0.11762739441735448 2312 9 0.017387593235973497 2312 10-11 0.19180063980661544 2312 12-13 -0.5156661427544904 2312 14-15 0.15137409118098333 2312 16-17 -0.08643487788209114 2312 18-19 0.10701091118423278 2312 20-21 0.14609802596524446 2312 22-23 0.4636896977679825 2312 24-25 0.33557091047674703 2312 26-27 0.3658469611162758 2312 28-29 -0.2824652920678403 2312 30-31 0.15389282380966307 2312 32-33 0.3078539398809781 2312 34-35 -0.4383214740499355 2312 36-37 0.2895448736898416 2312 38-39 0.4829366872421943 2312 40-41 0.4901616578500807 2312 42-43 0.3701859242902401 2312 44-45 0.5286888268322798 2312 46-47 0.530767657452067 2312 48-49 0.6250211998415658 2312 50-51 0.568964187061443 2312 52-53 0.49485519910208353 2312 54-55 0.48784328499941054 2312 56-57 0.5669446075460378 2312 58-59 0.4596140183090469 2312 60-61 0.6628482857802069 2312 62-63 0.44858472818871675 2312 64-65 0.35549813294599275 2312 66-67 0.2895506345025147 2312 68-69 0.6112448171797311 2312 70-71 0.6637464814798157 2312 72-73 0.48328112566499115 2312 74-75 0.756692338366939 2312 76-77 0.7836854566042071 2312 78-79 0.7770783285628156 2312 80-81 0.762927659771858 2312 82-83 0.6466931236763962 2312 84-85 0.6263528831605143 2312 86-87 0.6972220143349155 2312 88-89 1.2576968604125156 2312 90-91 1.4088700578013658 2312 92-93 1.993480631984113 2312 94-95 2.3019087506265024 2312 96-97 1.9714421796171457 2312 98-99 1.8105514288303617 2312 100-101 1.920355762767306 2313 1 -14.168824356905844 2313 2 -7.36065312955639 2313 3 -3.4805003371094863 2313 4 -0.9346229550454552 2313 5 -0.3690528225848837 2313 6 -0.8635416383241221 2313 7 -0.3845347551181746 2313 8 -0.0784470265965922 2313 9 -2.4490751198549816 2313 10-11 -0.5019867873781365 2313 12-13 -0.7524287147787234 2313 14-15 -0.8099078227869754 2313 16-17 -0.644192946589385 2313 18-19 -0.08971972449139543 2313 20-21 -0.9416523305382682 2313 22-23 -1.8341741610413749 2313 24-25 -1.0672531429973091 2313 26-27 -0.09277977968328344 2313 28-29 0.25722219552807246 2313 30-31 -1.2729306680878807 2313 32-33 -0.21963422632445173 2313 34-35 -1.2299981389826726 2313 36-37 -1.2491965166307537 2313 38-39 -0.5098186631051007 2313 40-41 -0.17238916515602654 2313 42-43 0.15475326548975943 2313 44-45 -0.06928140752833656 2313 46-47 -0.9095514332032266 2313 48-49 -0.4290566385663048 2313 50-51 -1.1433882795032986 2313 52-53 -0.31268244987602856 2313 54-55 -0.9900840012626055 2313 56-57 -0.7029672255096173 2313 58-59 -0.39966421945475616 2313 60-61 -1.8896244460706981 2313 62-63 -0.6423819990659432 2313 64-65 -0.4038377213555968 2313 66-67 -1.6949106627108144 2313 68-69 -0.5548053436909086 2313 70-71 -0.12356100828169403 2313 72-73 -0.9229076502507318 2313 74-75 -0.4428821783745889 2313 76-77 0.08376341229138617 2313 78-79 -0.001697375901670739 2313 80-81 -0.05058204471176353 2313 82-83 0.06595503707027106 2313 84-85 0.2520023125796378 2313 86-87 0.2658024620178807 2313 88-89 0.4060630450561824 2313 90-91 0.706078080880566 2313 92-93 1.2503771146185834 2313 94-95 1.1954809541805602 2313 96-97 0.8904789129546025 2313 98-99 0.9343177580553501 2313 100-101 0.8645619329661045 2314 1 -14.168824356905844 2314 2 -7.226635872786893 2314 3 -3.426683361227969 2314 4 -1.4535796687604297 2314 5 -1.3458341786950534 2314 6 -6.072191591899923 2314 7 -1.8811994119191446 2314 8 -4.4163109519544115 2314 9 -1.5627942506558838 2314 10-11 -0.4853492443199201 2314 12-13 -2.921551088283092 2314 14-15 -3.025120666046945 2314 16-17 -2.630446138638362 2314 18-19 -1.3333001290311728 2314 20-21 -2.2238444936175767 2314 22-23 -1.2344739146312733 2314 24-25 -2.964897483650862 2314 26-27 -4.5686286681346004 2314 28-29 -2.0231449165434228 2314 30-31 -1.8853908110472872 2314 32-33 -3.9047997670774457 2314 34-35 -2.749841413088987 2314 36-37 -0.9213868993806997 2314 38-39 -1.5269468637972636 2314 40-41 -2.6577098421212852 2314 42-43 -1.508823766193757 2314 44-45 -2.420894512080338 2314 46-47 -2.1055328779409805 2314 48-49 -1.213613689870428 2314 50-51 -2.21646354380443 2314 52-53 -0.9937957731989187 2314 54-55 -0.48772205337930785 2314 56-57 -1.0348181524712494 2314 58-59 -1.4282942299271113 2314 60-61 -0.6080154665332955 2314 62-63 -3.0274458514274123 2314 64-65 -1.324705116411252 2314 66-67 -2.417361583431333 2314 68-69 -2.147056424574675 2314 70-71 -2.6523075890676218 2314 72-73 -2.7263951893205167 2314 74-75 -2.112312671214717 2314 76-77 -0.9775089417735359 2314 78-79 -0.4169396130918095 2314 80-81 -0.3609637835595443 2314 82-83 -0.527842947999055 2314 84-85 -0.2658723080333125 2314 86-87 0.08237309807919502 2314 88-89 0.17479878262140502 2314 90-91 0.48342347037290523 2314 92-93 1.054945181769913 2314 94-95 1.0137486415440975 2314 96-97 0.9684238570515937 2314 98-99 1.131900692996119 2314 100-101 1.199691482990465 2315 1 -14.168824356905844 2315 2 -7.376099090622876 2315 3 -4.638400023497773 2315 4 -2.9942076484035276 2315 5 -3.28815990449435 2315 6 -2.802147653984406 2315 7 -3.302524161960328 2315 8 -3.6677611489300617 2315 9 -2.726583467703122 2315 10-11 -4.774839895512695 2315 12-13 -4.576140204260064 2315 14-15 -3.3039136905092477 2315 16-17 -4.540668856099746 2315 18-19 -1.5699041432716285 2315 20-21 -3.3261798137956475 2315 22-23 -4.449881350284425 2315 24-25 -5.147629982693729 2315 26-27 -4.714450073295406 2315 28-29 -2.733028864304849 2315 30-31 -3.4491106766914257 2315 32-33 -4.744392246943075 2315 34-35 -3.995106240599455 2315 36-37 -5.1296496236927425 2315 38-39 -3.7553753037862165 2315 40-41 -4.826631197399767 2315 42-43 -3.5662629329938653 2315 44-45 -2.085722512152529 2315 46-47 -1.4688659251901726 2315 48-49 -0.9719785788601811 2315 50-51 -1.0141206971174945 2315 52-53 -0.8279443503239392 2315 54-55 -2.3728388237082747 2315 56-57 -4.255018505449154 2315 58-59 -3.6799816943346 2315 60-61 -4.429414339246591 2315 62-63 -2.5236950771762103 2315 64-65 -3.283509480557573 2315 66-67 -1.9895255872114852 2315 68-69 -1.6087880630610947 2315 70-71 -2.1215677524466265 2315 72-73 -2.1768457526374654 2315 74-75 -2.7961885467953813 2315 76-77 -2.735957303970647 2315 78-79 -2.091929024138654 2315 80-81 -1.846567847336722 2315 82-83 -1.4660261178804213 2315 84-85 -1.1915191845897333 2315 86-87 -1.8403267278510889 2315 88-89 -2.234118699961062 2315 90-91 -1.8418160338912841 2315 92-93 -0.9698184244108852 2315 94-95 -0.7665542927575402 2315 96-97 -1.6887068476869196 2315 98-99 -1.0317393378932849 2315 100-101 -0.5496784118956928 2316 1 -14.168824356905844 2316 2 -7.363208190014092 2316 3 -5.16404919514126 2316 4 -2.8465234610102783 2316 5 -2.2455520754939684 2316 6 -1.282298905676008 2316 7 -4.811019009070044 2316 8 -2.935511535445027 2316 9 -1.281142223761094 2316 10-11 -4.662258977978219 2316 12-13 -3.894601354204738 2316 14-15 -2.10650767822559 2316 16-17 -3.429150420974498 2316 18-19 -2.6543890855739107 2316 20-21 -3.5322147485796833 2316 22-23 -3.145130589784902 2316 24-25 -3.584988148087433 2316 26-27 -4.458036623731271 2316 28-29 -3.988688850656338 2316 30-31 -3.0576587298512052 2316 32-33 -3.2729364253144304 2316 34-35 -4.45023368451141 2316 36-37 -2.5520092734581326 2316 38-39 -3.5906616140237553 2316 40-41 -3.1593974493419807 2316 42-43 -6.887645596303663 2316 44-45 -3.8305974779348695 2316 46-47 -5.006529928983998 2316 48-49 -4.432546919718188 2316 50-51 -3.378552549035035 2316 52-53 -4.824867459870571 2316 54-55 -4.827125187922963 2316 56-57 -4.828278513391506 2316 58-59 -3.723185488928401 2316 60-61 -3.3913304703185396 2316 62-63 -2.46952996877852 2316 64-65 -2.387751506397212 2316 66-67 -2.717978582750657 2316 68-69 -2.800357795758522 2316 70-71 -3.048356998655695 2316 72-73 -3.7697889146961536 2316 74-75 -4.411582830614957 2316 76-77 -4.0762532722629246 2316 78-79 -4.309253183926447 2316 80-81 -4.134886516877032 2316 82-83 -3.6117316570928963 2316 84-85 -3.3304015298198912 2316 86-87 -3.578415416167733 2316 88-89 -3.334621554966862 2316 90-91 -2.5472286892201765 2316 92-93 -2.5248164195452354 2316 94-95 -1.642097052300894 2316 96-97 -1.8907407260609723 2316 98-99 -1.778085855788639 2316 100-101 -1.5840515443826124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19473.0 3 5910.0 4 6353.0 5 7751.0 6 8948.0 7 11104.0 8 14159.0 9 17192.0 10 20636.0 11 24597.0 12 29397.0 13 32428.0 14 35226.0 15 39388.0 16 44651.0 17 46515.0 18 41347.0 19 41025.0 20 43675.0 21 47740.0 22 53116.0 23 58515.0 24 65753.0 25 73513.0 26 82514.0 27 93093.0 28 104242.0 29 118462.0 30 134932.0 31 154798.0 32 176197.0 33 203629.0 34 237376.0 35 277670.0 36 297063.0 37 184013.0 38 24010.0 39 118.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.253152297295195 19.404556605107796 13.746822527272768 22.595468570324236 2 15.045519784970555 21.31087934168979 40.90371517297171 22.73988570036795 3 18.70127365899993 26.819896967032726 30.537952379013184 23.940876994954163 4 11.916987635641176 17.222920006102417 39.358772606282 31.501319751974403 5 12.689672455530632 38.66904277517407 34.710629314890646 13.930655454404647 6 29.47022173202205 37.915312349423594 18.824835601901754 13.789630316652602 7 26.928851365843197 33.956843700297206 22.304802943658792 16.809501990200804 8 25.6910814242675 34.61912748348002 20.9873581583557 18.70243293389677 9 27.178304423885326 16.44202299410787 21.08477880573373 35.29489377627307 10-11 24.14208824821933 26.940185964347407 28.69585648460377 20.221869302829496 12-13 25.78131881024187 24.955343512912002 28.554947061479563 20.708390615366564 14-15 22.73928014938673 25.68198082601031 27.553510255715285 24.025228768887676 16-17 21.440988737586515 29.113132589282838 27.834616015948864 21.611262657181783 18-19 21.165960187659394 27.71878944024232 30.52793565963669 20.587314712461595 20-21 22.335005050674784 26.77038732171797 30.19215201233824 20.702455615269006 22-23 21.895710726067232 26.947749820132955 29.967199844089098 21.189339609710714 24-25 21.839192395863584 26.985740240785113 30.237142340978153 20.937925022373154 26-27 21.634073947361223 27.088471808768322 30.350710910380922 20.92674333348953 28-29 21.158291110211884 27.271691611506753 30.524725675275267 21.045291603006095 30-31 21.44260370080647 27.065383469545267 30.355167313317644 21.136845516330624 32-33 21.27651086635001 27.25625228425565 30.24053103086171 21.226705818532636 34-35 21.858804967854006 27.09622164614726 30.00582984143772 21.03914354456101 36-37 21.859873387960533 26.836875974189955 30.080042494274593 21.22320814357492 38-39 21.40877187513551 27.34544101878847 30.15802499149715 21.087762114578872 40-41 21.57808943464639 27.06078121652993 30.29238584691583 21.06874350190785 42-43 21.413484640545242 27.25314754629917 29.70173734769017 21.63163046546541 44-45 21.617949035023397 27.320237077185936 29.495923603043757 21.565890284746907 46-47 21.777069466709825 27.460487635933994 29.07869929837484 21.68374359898134 48-49 21.903673780564823 27.597910831407773 28.883231406310678 21.615183981716726 50-51 21.58888437808726 27.755078572162855 28.912499934661135 21.74353711508875 52-53 21.90118589268214 27.938811053831124 28.58574241294818 21.574260640538554 54-55 21.429446470428427 27.6520471112578 28.778438829234325 22.14006758907945 56-57 21.789729384961387 28.077139981508232 28.412619073212873 21.720511560317508 58-59 21.999579966271916 27.553289602458626 28.504129635086123 21.943000796183334 60-61 21.90188473667555 27.687866275079216 28.85951112176987 21.550737866475366 62-63 21.745371057378197 27.638812543934634 28.86034342340761 21.75547297527956 64-65 21.539777307611864 27.68350562268936 28.773544407604007 22.00317266209477 66-67 21.529226984735736 27.969383692411547 28.31675794711133 22.184631375741382 68-69 21.55587617842692 27.718673457215274 28.65000840256378 22.07544196179402 70-71 21.52688406891342 27.752200940412504 28.65993918338392 22.060975807290156 72-73 21.73420953564216 27.50745203409043 28.550031278422555 22.208307151844853 74-75 21.44146028011937 28.123775294604542 28.23244244048008 22.202321984796008 76-77 21.6264872439053 27.60623718426481 28.486912462745384 22.280363109084504 78-79 21.529625929695563 27.74115888556592 28.43335440030487 22.295860784433653 80-81 21.562202014175064 28.023040054871213 28.180624020654776 22.234133910298947 82-83 21.63790907389667 27.910105413336744 28.126671640714378 22.325313872052206 84-85 21.471677282688578 27.740068502039154 28.011108457613688 22.777145757658577 86-87 21.475479063635206 27.673080661919293 28.280241861022592 22.57119841342291 88-89 21.6623362505135 27.509199448503473 28.08829170918976 22.740172591793268 90-91 21.42523442627748 27.616471084987104 28.324993067197322 22.633301421538093 92-93 21.538853326556275 27.632545000929294 28.39209000203277 22.436511670481664 94-95 21.458997496835103 27.347037282606706 28.221271761111115 22.972693459447076 96-97 21.508161128447853 27.250801070173626 28.15245494475958 23.08858285661894 98-99 21.380951666988032 27.36299742290418 27.79570962884032 23.460341281267468 100-101 21.361294130896308 27.22875909970653 28.24246614555277 23.167480623844394 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4140.0 1 2789.0 2 1356.5 3 1104.5 4 940.5 5 965.0 6 1328.0 7 2779.0 8 3804.0 9 3535.0 10 3187.5 11 3011.0 12 3197.0 13 3503.0 14 3741.0 15 4006.5 16 4284.0 17 4638.0 18 5085.5 19 5555.5 20 6140.5 21 7209.5 22 8622.0 23 10226.0 24 12285.5 25 15611.5 26 20357.5 27 25195.0 28 29495.0 29 34722.5 30 41252.5 31 48421.0 32 55939.5 33 64675.0 34 73754.5 35 80403.5 36 87743.0 37 93455.5 38 93967.0 39 92691.5 40 92495.0 41 93812.0 42 95258.0 43 97879.5 44 100026.5 45 100104.5 46 100757.5 47 108376.5 48 115140.5 49 110054.0 50 100038.0 51 92180.5 52 87266.5 53 83475.5 54 78627.0 55 71392.0 56 63573.0 57 57202.0 58 51653.5 59 45484.5 60 38600.5 61 31896.0 62 25591.5 63 19517.0 64 14917.0 65 11140.5 66 8050.5 67 5886.0 68 3938.0 69 2693.0 70 1863.5 71 1320.5 72 927.0 73 657.0 74 538.5 75 391.5 76 232.5 77 152.0 78 108.5 79 73.0 80 42.0 81 26.5 82 25.0 83 13.5 84 9.5 85 11.0 86 7.5 87 4.5 88 5.5 89 6.0 90 4.0 91 4.0 92 5.0 93 2.5 94 3.5 95 5.5 96 5.0 97 4.0 98 3.0 99 2.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.050256055127555 2 0.08704935705497842 3 0.1205619689563359 4 0.19241940547096867 5 0.1211181948800099 6 0.14715652093199827 7 0.15494368386343405 8 0.15296212901034545 9 0.14093374341089557 10-11 0.12746264682191627 12-13 0.14065563044905857 14-15 0.14783442127647592 16-17 0.14248074676111383 18-19 0.14962477346830155 20-21 0.17835731883808575 22-23 0.1967127743193272 24-25 0.22455883462325602 26-27 0.21659785109067212 28-29 0.2142338909150577 30-31 0.22992989119873294 32-33 0.2203349940153567 34-35 0.20696818978706627 36-37 0.1930799237553315 38-39 0.18885608314743219 40-41 0.1909940765415541 42-43 0.18371099335344787 44-45 0.19933746539666383 46-47 0.22520197084750404 48-49 0.2288695855317294 50-51 0.23896856245843515 52-53 0.266901533062938 54-55 0.253030649091318 56-57 0.2295648679363219 58-59 0.18532752494412535 60-61 0.19643466135749024 62-63 0.2011799637688339 64-65 0.18906466786880993 66-67 0.19836407003023435 68-69 0.1870831130157214 70-71 0.20050206342435622 72-73 0.22038714019570116 74-75 0.25087527363708134 76-77 0.25216154608557745 78-79 0.24712074865228198 80-81 0.25256133346821813 82-83 0.2422363897600198 84-85 0.2121828078215099 86-87 0.2242633396013042 88-89 0.22666206389714824 90-91 0.21265212344460982 92-93 0.21068795065163604 94-95 0.24896324702445202 96-97 0.2788951545421583 98-99 0.2979980386083366 100-101 0.29347870297848555 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2876529.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.22962043251005 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50740095026235 55.469412487590574 2 10.492911949570685 14.108689752027722 3 3.2057307855593344 6.46560191765899 4 1.3843119245221565 3.7226706098328837 5 0.7129218483855727 2.396473263250277 6 0.402193783721442 1.6223601251944548 7 0.2606170892139754 1.2264831590256824 8 0.1801453018047395 0.9688880210426085 9 0.1296149427043369 0.7842567060354698 >10 0.6617908901850342 7.89063336429989 >50 0.04207271674638473 1.978558218549663 >100 0.019269955939368855 2.378963990312887 >500 7.184903890850025E-4 0.3493977480979135 >1k 2.394967963612087E-4 0.39907019293060964 >5k 5.9874199090302176E-5 0.23854044415037248 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5926 0.20601217648075162 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3920 0.13627535130012594 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.952824045924793E-5 0.0 5 0.0 0.0 0.0 6.952824045924793E-5 0.0 6 0.0 0.0 0.0 6.952824045924793E-5 0.0 7 0.0 0.0 0.0 6.952824045924793E-5 0.0 8 0.0 0.0 0.0 6.952824045924793E-5 0.0 9 0.0 0.0 0.0 6.952824045924793E-5 0.0 10-11 0.0 0.0 0.0 1.042923606888719E-4 0.0 12-13 0.0 0.0 0.0 1.3905648091849587E-4 0.0 14-15 0.0 0.0 0.0 1.3905648091849587E-4 0.0 16-17 0.0 0.0 0.0 1.3905648091849587E-4 0.0 18-19 0.0 0.0 0.0 1.7382060114811982E-4 0.0 20-21 0.0 0.0 0.0 2.2596678149255578E-4 0.0 22-23 0.0 0.0 0.0 5.040797433295475E-4 0.0 24-25 0.0 0.0 0.0 0.001060305667003531 0.0 26-27 0.0 0.0 0.0 0.0017729701317108224 0.0 28-29 0.0 0.0 0.0 0.004606245930425175 0.0 30-31 0.0 0.0 0.0 0.012410790921975756 1.7382060114811984E-5 32-33 0.0 0.0 0.0 0.028541342708521277 3.476412022962397E-5 34-35 0.0 0.0 0.0 0.05119016703812129 3.476412022962397E-5 36-37 0.0 0.0 0.0 0.08517209456257871 3.476412022962397E-5 38-39 0.0 0.0 0.0 0.13869145765608482 3.476412022962397E-5 40-41 0.0 0.0 1.7382060114811984E-5 0.1990072062544824 3.476412022962397E-5 42-43 0.0 0.0 3.476412022962397E-5 0.25845385184713937 3.476412022962397E-5 44-45 0.0 0.0 3.476412022962397E-5 0.3289902517930464 3.476412022962397E-5 46-47 0.0 0.0 3.476412022962397E-5 0.4161439012087137 3.476412022962397E-5 48-49 0.0 0.0 3.476412022962397E-5 0.5107718364737501 3.476412022962397E-5 50-51 0.0 0.0 3.476412022962397E-5 0.6164373799116922 3.476412022962397E-5 52-53 0.0 0.0 3.476412022962397E-5 0.7216162256664196 3.476412022962397E-5 54-55 0.0 0.0 3.476412022962397E-5 0.8333307260243161 3.476412022962397E-5 56-57 0.0 0.0 5.214618034443595E-5 0.9664599244436611 3.476412022962397E-5 58-59 0.0 0.0 6.952824045924793E-5 1.1130776015121002 3.476412022962397E-5 60-61 0.0 0.0 6.952824045924793E-5 1.2847254451458685 3.476412022962397E-5 62-63 0.0 0.0 6.952824045924793E-5 1.457642179168018 3.476412022962397E-5 64-65 0.0 0.0 6.952824045924793E-5 1.6290119098399494 3.476412022962397E-5 66-67 1.7382060114811984E-5 0.0 6.952824045924793E-5 1.824021242268025 3.476412022962397E-5 68-69 3.476412022962397E-5 0.0 6.952824045924793E-5 2.0520912530344733 3.476412022962397E-5 70-71 3.476412022962397E-5 0.0 6.952824045924793E-5 2.310058407198398 3.476412022962397E-5 72-73 3.476412022962397E-5 0.0 6.952824045924793E-5 2.5661656809300375 3.476412022962397E-5 74-75 3.476412022962397E-5 0.0 6.952824045924793E-5 2.8361264565731825 3.476412022962397E-5 76-77 3.476412022962397E-5 0.0 6.952824045924793E-5 3.119836441767144 3.476412022962397E-5 78-79 3.476412022962397E-5 0.0 6.952824045924793E-5 3.4412828794703616 3.476412022962397E-5 80-81 3.476412022962397E-5 0.0 6.952824045924793E-5 3.7997183410978996 3.476412022962397E-5 82-83 3.476412022962397E-5 0.0 6.952824045924793E-5 4.170147424204657 3.476412022962397E-5 84-85 3.476412022962397E-5 0.0 6.952824045924793E-5 4.542401623623471 3.476412022962397E-5 86-87 3.476412022962397E-5 0.0 6.952824045924793E-5 4.92901340469712 3.476412022962397E-5 88-89 3.476412022962397E-5 0.0 6.952824045924793E-5 5.344183910539403 3.476412022962397E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11290 0.0 32.524845 1 GTATCAA 18610 0.0 31.192183 1 TACATGG 13960 0.0 27.783068 2 ACATGGG 13615 0.0 27.100107 3 CATGGGA 9490 0.0 25.285677 4 TATCAAC 23935 0.0 24.059841 2 GGTATCA 13765 0.0 23.753273 1 ATCAACG 25020 0.0 22.941751 3 TCAACGC 25230 0.0 22.78507 4 GAGTACT 10205 0.0 22.730515 12-13 CAACGCA 25315 0.0 22.614616 5 AACGCAG 25765 0.0 22.219637 6 ACGCAGA 26490 0.0 21.308632 7 AGTACTT 11250 0.0 20.430323 12-13 ATGGGAG 4075 0.0 20.268276 5 GTACTTT 11520 0.0 20.159662 14-15 CGCAGAG 27690 0.0 20.146206 8 ATGGGAT 4245 0.0 19.567768 5 CATGGGG 4395 0.0 19.560932 4 CGCGGAA 380 0.0 19.267826 82-83 >>END_MODULE