FastQCFastQC Report
Fri 27 May 2016
SRR939668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876529
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145810.506895637068147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109450.3804932959132343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75000.2607309017221798No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53500.18598804322848822No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30090.10460523777093853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG142500.027.1952061
TACATGG141950.026.7278372
GGTATCA138250.026.1533721
GTATCAA196600.026.1221831
ACATGGG139300.025.5367243
CATGGGA94950.022.9060864
CATTCCG5450.022.4935239
ATCAACG232100.021.9326573
TCAACGC233700.021.7824974
CAACGCA235650.021.6022475
AACGCAG237050.021.4616016
TATCAAC241250.021.203382
CATGGGG47050.021.0573944
GAGTACT111600.020.8713712-13
AGTACTT117550.020.43665312-13
ATGGGAG40600.019.4059725
AGAGTAC198650.019.2136110-11
AGTACAT104550.019.1826272
ACGCAGA262700.019.0609747
CGCAGAG259900.018.9942048
Produced by FastQC (version 0.11.5)