##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939668_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2876529 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48213871648782 31.0 31.0 34.0 30.0 34.0 2 31.67609747720256 31.0 31.0 34.0 30.0 34.0 3 31.723179915794347 31.0 31.0 34.0 30.0 34.0 4 35.52994007708596 37.0 35.0 37.0 33.0 37.0 5 35.290401035414554 37.0 35.0 37.0 33.0 37.0 6 35.30193333701833 37.0 35.0 37.0 32.0 37.0 7 35.21070707091776 37.0 35.0 37.0 32.0 37.0 8 35.173274456819314 37.0 35.0 37.0 32.0 37.0 9 36.7630195975775 39.0 35.0 39.0 32.0 39.0 10-11 36.64370878930822 39.0 35.0 39.0 32.0 39.0 12-13 36.579395862165825 39.0 35.0 39.0 32.0 39.0 14-15 37.6275361729362 39.0 36.0 41.0 32.0 41.0 16-17 37.592381825457 39.0 36.0 41.0 32.0 41.0 18-19 37.591108068091785 39.0 36.0 41.0 32.0 41.0 20-21 37.545855786609486 39.0 36.0 41.0 32.0 41.0 22-23 37.40412333753632 39.0 36.0 41.0 32.0 41.0 24-25 37.27462698968097 39.0 36.0 41.0 31.0 41.0 26-27 36.99110073286242 39.0 36.0 40.0 31.0 41.0 28-29 36.79394123959814 39.0 36.0 40.0 30.0 41.0 30-31 36.535363453662384 38.0 35.0 40.0 30.0 41.0 32-33 36.34869976975723 38.0 35.0 40.0 30.0 41.0 34-35 36.297376803779834 38.0 35.0 40.0 30.0 41.0 36-37 36.190612540322036 38.0 35.0 40.0 30.0 41.0 38-39 36.21494655537977 38.0 35.0 40.0 30.0 41.0 40-41 36.10327481488975 38.0 35.0 40.0 30.0 41.0 42-43 35.90328187200616 38.0 35.0 40.0 29.0 41.0 44-45 35.66020279997177 38.0 35.0 40.0 28.0 41.0 46-47 35.32322097222034 38.0 34.0 40.0 27.0 41.0 48-49 34.99891588091064 38.0 34.0 40.0 26.0 41.0 50-51 34.75527276102552 38.0 34.0 40.0 25.0 41.0 52-53 34.53447835916134 38.0 33.0 40.0 24.0 41.0 54-55 34.25626040968125 38.0 33.0 40.0 24.0 41.0 56-57 33.46909678296308 37.5 32.5 40.0 20.5 41.0 58-59 30.41624871503121 35.5 26.5 39.0 8.0 40.5 60-61 30.16745250960446 34.5 26.0 39.0 10.0 40.0 62-63 31.976784694331258 35.5 30.5 39.0 16.5 40.0 64-65 32.037652670979504 36.0 31.0 39.0 11.0 40.0 66-67 27.184469372636258 32.0 18.5 37.0 2.0 39.5 68-69 19.731368082852633 20.0 8.0 32.5 2.0 37.0 70-71 17.791414930981055 15.5 7.5 29.0 2.0 35.0 72-73 19.508028078284624 20.0 7.5 31.0 2.0 35.5 74-75 20.953093989318376 23.5 9.0 32.5 2.0 36.0 76-77 22.769017972702517 27.5 11.5 33.0 2.0 35.5 78-79 24.93672738915547 30.5 13.0 34.5 2.0 37.0 80-81 26.530254170912237 32.0 22.5 35.0 2.0 37.0 82-83 27.658822142936852 33.0 26.5 35.0 2.0 36.5 84-85 27.698058319592818 34.0 27.0 35.0 2.0 36.0 86-87 27.416551684339005 33.5 26.5 35.0 2.0 36.0 88-89 26.98068766210944 33.0 26.0 35.0 2.0 35.5 90-91 25.15701006316988 32.0 16.0 34.5 2.0 35.0 92-93 23.558577542586917 31.0 2.0 34.0 2.0 35.0 94-95 19.814580002496065 27.5 2.0 34.0 2.0 35.0 96-97 17.122454353841036 18.0 2.0 33.0 2.0 35.0 98-99 17.396742740990966 21.5 2.0 33.0 2.0 35.0 100-101 16.624082705232592 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.24202131827929918 1101 2 0.2789269166499615 1101 3 0.20574834436859746 1101 4 0.19205426640314016 1101 5 0.3532210713978614 1101 6 0.3448042591877041 1101 7 0.3766723530656577 1101 8 0.40653041214265073 1101 9 0.36000558270289673 1101 10-11 0.320495333707278 1101 12-13 0.2542850185259198 1101 14-15 0.44897996661234885 1101 16-17 0.3804388065065325 1101 18-19 0.4155938315012975 1101 20-21 0.37688335268817497 1101 22-23 0.35160918786033335 1101 24-25 0.29129536238350795 1101 26-27 0.4433557444620462 1101 28-29 0.42332792323031043 1101 30-31 0.3153459557201188 1101 32-33 0.3152536726180628 1101 34-35 0.19214576700913 1101 36-37 0.053022434486720726 1101 38-39 0.19735652230524892 1101 40-41 0.2529091570427582 1101 42-43 0.3289976860307391 1101 44-45 0.27210195528108017 1101 46-47 0.1553406474596457 1101 48-49 0.18305077325040742 1101 50-51 0.2045257040641033 1101 52-53 0.09306882942971129 1101 54-55 0.23871645690812926 1101 56-57 -3.3776942594408013 1101 58-59 -10.091600256224677 1101 60-61 -11.635549675718579 1101 62-63 -3.8641408150833385 1101 64-65 -1.0827277511317277 1101 66-67 -1.9128447955923278 1101 68-69 -4.577760920064975 1101 70-71 -4.345152339320883 1101 72-73 -3.4123512370466003 1101 74-75 -6.113574340999831 1101 76-77 -4.829251669498131 1101 78-79 1.2111519116940777 1101 80-81 1.9167301024756078 1101 82-83 0.5530416593874605 1101 84-85 0.42635712794776737 1101 86-87 0.32488841165031346 1101 88-89 0.11867242957472612 1101 90-91 1.6258431948553635 1101 92-93 1.1299366737998184 1101 94-95 -8.501469616178053 1101 96-97 -9.49088189355686 1101 98-99 -8.453837932426776 1101 100-101 -9.29015634912616 1102 1 0.26353335136528955 1102 2 0.26953389296461694 1102 3 0.22465720747570472 1102 4 0.22375786347612348 1102 5 0.24651752192787058 1102 6 0.2924381045388884 1102 7 0.2797519338546124 1102 8 0.254532609497069 1102 9 0.24177734726989542 1102 10-11 0.3596084565211157 1102 12-13 0.31447198882355565 1102 14-15 0.337220026630213 1102 16-17 0.43108101856075365 1102 18-19 0.3644381020995979 1102 20-21 0.25640480450346814 1102 22-23 0.2696894255706681 1102 24-25 0.21899200085015735 1102 26-27 0.08424193616503572 1102 28-29 0.16517709979364525 1102 30-31 0.253696859320236 1102 32-33 -0.0777000363219642 1102 34-35 -0.018864282935929566 1102 36-37 -0.17110807294219654 1102 38-39 -0.02304976452757046 1102 40-41 -0.15295091489489465 1102 42-43 0.030707538137740187 1102 44-45 -0.14201283363770045 1102 46-47 -0.1690127372087602 1102 48-49 0.004256409635857494 1102 50-51 0.04695144814657226 1102 52-53 -7.697716514556419E-4 1102 54-55 -0.3872582144883694 1102 56-57 -3.60305470934626 1102 58-59 -9.72521413783031 1102 60-61 -8.098094922368759 1102 62-63 -2.499431393307443 1102 64-65 -0.9274975858731551 1102 66-67 -1.8325943514563505 1102 68-69 -4.861686149083003 1102 70-71 -4.8148607661941085 1102 72-73 -3.6296090615514807 1102 74-75 -4.12893963689055 1102 76-77 -4.015491975147462 1102 78-79 1.8255707171634725 1102 80-81 2.062944140790311 1102 82-83 0.7486636830099727 1102 84-85 0.4245945079168081 1102 86-87 0.3686172402166825 1102 88-89 0.23656744401747787 1102 90-91 1.5540795323187346 1102 92-93 1.1900497404421095 1102 94-95 -8.626502462247092 1102 96-97 -9.616865252500606 1102 98-99 -8.692982306609196 1102 100-101 -9.571526725208889 1103 1 0.31376719303415257 1103 2 0.22589962353213622 1103 3 0.2737648735270959 1103 4 0.22310138549423186 1103 5 0.26722633503032966 1103 6 0.31703634367632816 1103 7 0.2237205767305852 1103 8 0.2609682911354625 1103 9 0.35324218129174767 1103 10-11 0.31999728925381987 1103 12-13 0.32332299670495246 1103 14-15 0.39872432257818247 1103 16-17 0.32994999031663497 1103 18-19 0.3768104688629421 1103 20-21 0.37043695269718313 1103 22-23 0.4003601318201149 1103 24-25 0.4065542609655921 1103 26-27 0.48251211210479283 1103 28-29 0.45258398429746904 1103 30-31 0.3657664404927843 1103 32-33 0.2679873519760676 1103 34-35 0.28964237157170913 1103 36-37 0.47435536205824036 1103 38-39 0.35199967732880566 1103 40-41 0.27488228659348835 1103 42-43 0.3629453681163568 1103 44-45 0.17325316354961018 1103 46-47 0.22145798588754673 1103 48-49 0.12237987144477103 1103 50-51 0.021917388201067922 1103 52-53 0.2788880174552233 1103 54-55 0.12350546076286406 1103 56-57 0.6809594566611779 1103 58-59 -8.019074557976214 1103 60-61 -7.167080045863528 1103 62-63 -1.854474737334865 1103 64-65 -0.12258645303204574 1103 66-67 -1.596947597039545 1103 68-69 -3.547259747864169 1103 70-71 -4.148989747179137 1103 72-73 -3.167173885318732 1103 74-75 -3.598652379705001 1103 76-77 -0.46609132031754186 1103 78-79 3.3285752255283008 1103 80-81 2.77507625675527 1103 82-83 1.1881289968009554 1103 84-85 0.7894104010931002 1103 86-87 0.6434695555605039 1103 88-89 0.5401747767941316 1103 90-91 1.8958111143116447 1103 92-93 1.7089475166572825 1103 94-95 -6.889865039522883 1103 96-97 -8.359570747505147 1103 98-99 -7.683656565347587 1103 100-101 -9.23006203768142 1104 1 0.26835766640819614 1104 2 0.27501904071299066 1104 3 0.2493032405891249 1104 4 0.16811874272006122 1104 5 0.29974082824729464 1104 6 0.15454247132321086 1104 7 0.24995190072858264 1104 8 0.27778901483719665 1104 9 0.3534676387339104 1104 10-11 0.2323716058879839 1104 12-13 0.25796797949426065 1104 14-15 0.39617152490775 1104 16-17 0.3940443440896715 1104 18-19 0.3280087568516947 1104 20-21 0.29955173417997116 1104 22-23 0.33192280832299303 1104 24-25 0.3225628716415514 1104 26-27 0.3319123686666998 1104 28-29 0.27141993786588614 1104 30-31 0.3943743923134875 1104 32-33 0.41459163653332354 1104 34-35 0.2770808619859295 1104 36-37 0.16564717658769013 1104 38-39 0.09942206934901066 1104 40-41 0.20169953352166914 1104 42-43 0.24686892039317598 1104 44-45 0.3893183217494638 1104 46-47 0.22825534460532282 1104 48-49 0.1490096590019121 1104 50-51 0.17770281932099152 1104 52-53 0.1028873460782691 1104 54-55 0.09050008974717372 1104 56-57 0.4443237212902744 1104 58-59 -8.260179479275916 1104 60-61 -7.828622467829277 1104 62-63 -2.3129424459679235 1104 64-65 -0.4053815192106285 1104 66-67 -1.7438521029913545 1104 68-69 -3.5660163417450477 1104 70-71 -4.0039999643642545 1104 72-73 -2.918770403749626 1104 74-75 -3.400836119860802 1104 76-77 -0.09470829709642459 1104 78-79 3.646426093256384 1104 80-81 2.943946545127659 1104 82-83 1.4585681958908694 1104 84-85 1.088647042822906 1104 86-87 1.111265940515267 1104 88-89 0.9382045718110241 1104 90-91 2.240211677255896 1104 92-93 2.1572434979866095 1104 94-95 -6.336324891446964 1104 96-97 -7.646746581664381 1104 98-99 -5.668130473560764 1104 100-101 -5.073020771975997 1105 1 0.21582899618269735 1105 2 0.2411238121188326 1105 3 0.20152069287909669 1105 4 0.14800423385793948 1105 5 0.3415050295391566 1105 6 0.2569241563513174 1105 7 0.23399193680033648 1105 8 0.1415707907633319 1105 9 0.3053443867623642 1105 10-11 0.2658747904765235 1105 12-13 0.21080009319562265 1105 14-15 0.3356923808213139 1105 16-17 0.4170594123862301 1105 18-19 0.39881002053893155 1105 20-21 0.2599665048693467 1105 22-23 0.3484208868119296 1105 24-25 0.3379039820478056 1105 26-27 0.25361158244452753 1105 28-29 0.35109208868430386 1105 30-31 0.29966644740370185 1105 32-33 0.3575425100058496 1105 34-35 0.32841940834490657 1105 36-37 0.4993197442216726 1105 38-39 0.4511049785387513 1105 40-41 0.40277335626268496 1105 42-43 0.33675152823004595 1105 44-45 0.34873945334057765 1105 46-47 0.43175362279117024 1105 48-49 0.45116207369029127 1105 50-51 0.4315663664476048 1105 52-53 0.5049292632643727 1105 54-55 0.39599165663616276 1105 56-57 1.007444330598851 1105 58-59 -8.504060576646342 1105 60-61 -7.560845196734306 1105 62-63 -1.972162780905581 1105 64-65 0.06509241141462141 1105 66-67 -1.4442464227141585 1105 68-69 -3.2428180618823568 1105 70-71 -3.737249107050417 1105 72-73 -2.6287744020431667 1105 74-75 -3.3253781834985645 1105 76-77 0.131571325609265 1105 78-79 4.072647668841011 1105 80-81 3.444634214595091 1105 82-83 1.903139456217886 1105 84-85 1.5022710538386832 1105 86-87 1.38418355585112 1105 88-89 1.2764609129499576 1105 90-91 2.690898355860824 1105 92-93 2.4378373033755487 1105 94-95 -5.991236879990415 1105 96-97 -7.6866211210494235 1105 98-99 -5.753522568742799 1105 100-101 -5.1537820549055695 1106 1 0.07958285692203404 1106 2 0.07904312359254106 1106 3 0.12405407855306905 1106 4 0.11019349580700322 1106 5 0.14759690478777543 1106 6 0.10612277382649893 1106 7 0.1519641059291459 1106 8 0.1566619301351011 1106 9 0.10434130252777862 1106 10-11 0.047608662726737805 1106 12-13 0.14325300295899268 1106 14-15 0.13991591746549403 1106 16-17 0.19924084502387274 1106 18-19 0.09468277091896482 1106 20-21 0.15360713903921663 1106 22-23 0.18991283900302847 1106 24-25 0.1735663213170966 1106 26-27 0.24809424881957653 1106 28-29 0.32912339808762425 1106 30-31 0.3015051592314535 1106 32-33 0.29919115734742974 1106 34-35 0.27992473179741495 1106 36-37 0.1530486226172627 1106 38-39 0.14712738770980138 1106 40-41 0.24973286473703382 1106 42-43 0.30856263051605737 1106 44-45 0.3634055791733388 1106 46-47 0.4014489157498886 1106 48-49 0.27778737469616743 1106 50-51 0.20363417603979883 1106 52-53 0.3132879136949853 1106 54-55 0.25833780979527177 1106 56-57 0.8500215813298198 1106 58-59 -8.2239961514777 1106 60-61 -7.2492596239288964 1106 62-63 -1.7622355015716948 1106 64-65 -0.0335997188856858 1106 66-67 0.5548447323423709 1106 68-69 -2.0490955401731163 1106 70-71 -2.5015602561728763 1106 72-73 -1.1224739194316875 1106 74-75 -0.8732626791527984 1106 76-77 2.3667905049388374 1106 78-79 4.48824196237527 1106 80-81 3.5796945203347157 1106 82-83 1.924939208834303 1106 84-85 1.414988500219664 1106 86-87 1.1771222314732022 1106 88-89 1.1114285478748727 1106 90-91 2.46389766616689 1106 92-93 2.3057270403551264 1106 94-95 -5.9798900423656285 1106 96-97 -7.823005700137887 1106 98-99 -5.693785292898772 1106 100-101 -5.1370595911428225 1107 1 0.037992289864277495 1107 2 -6.203822007684323E-4 1107 3 0.04130397262241203 1107 4 0.05557259863236652 1107 5 0.04518559646467679 1107 6 -0.010482812608188397 1107 7 0.030524815582886333 1107 8 0.07785317514905188 1107 9 0.09890274101881147 1107 10-11 0.048379614435248186 1107 12-13 0.10557800250776239 1107 14-15 0.05775928104959149 1107 16-17 0.020765417068311365 1107 18-19 -0.014911516818784776 1107 20-21 0.04704487458987927 1107 22-23 0.18640466493811658 1107 24-25 0.07055198178721866 1107 26-27 0.06323483362555038 1107 28-29 0.03753734328903846 1107 30-31 0.01290256721789973 1107 32-33 0.027690027563686215 1107 34-35 0.1608025939663733 1107 36-37 0.11608215428945812 1107 38-39 0.14868787733719557 1107 40-41 0.24425670106837316 1107 42-43 0.1028269259976966 1107 44-45 -0.025120562277820113 1107 46-47 0.22773402864660852 1107 48-49 0.2325964545648631 1107 50-51 0.2941052945729794 1107 52-53 0.14517155843149965 1107 54-55 0.1306593542427379 1107 56-57 0.5171636109182103 1107 58-59 -8.505805859624775 1107 60-61 -7.651264313240517 1107 62-63 -2.096597021197443 1107 64-65 -0.31892984865825724 1107 66-67 0.7460781433438619 1107 68-69 -2.69212652779062 1107 70-71 -2.5806401764477034 1107 72-73 -0.8653129641391502 1107 74-75 -0.8278271301139917 1107 76-77 2.198765898945009 1107 78-79 4.388816802705758 1107 80-81 3.278668497061769 1107 82-83 1.6108128079927013 1107 84-85 1.2343059329246913 1107 86-87 0.9970670830714461 1107 88-89 0.9306909739920002 1107 90-91 2.3449527956601344 1107 92-93 2.108723094491925 1107 94-95 -6.943288744725841 1107 96-97 -8.244066304505582 1107 98-99 -5.966588639190167 1107 100-101 -5.365676209836295 1108 1 -0.1658438609809778 1108 2 -0.17909892576383868 1108 3 -0.13327340058955173 1108 4 -0.10535067835073164 1108 5 -0.14857253097355283 1108 6 -0.24260244849895685 1108 7 -0.14372746009981796 1108 8 -0.194903651899061 1108 9 -0.2177422524792405 1108 10-11 -0.2494097223657903 1108 12-13 -0.15206178942981552 1108 14-15 -0.2665255043860455 1108 16-17 -0.2635323722684788 1108 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0.1302526924345777 2312 88-89 0.2880150598842306 2312 90-91 1.891355336687301 2312 92-93 3.31093495916004 2312 94-95 5.342514735770109 2312 96-97 0.10524613474513345 2312 98-99 -0.8181377634593261 2312 100-101 -0.22487704061216718 2313 1 -0.33788765344815275 2313 2 -0.3616527383548629 2313 3 -0.24934701843005413 2313 4 -0.21511024417802815 2313 5 -0.24076893028092172 2313 6 -0.1644261426388809 2313 7 -0.32418558861804314 2313 8 -0.40666344252465336 2313 9 -0.4580335262655595 2313 10-11 -0.3898193552525768 2313 12-13 -0.35090182824551874 2313 14-15 -0.45535081931364374 2313 16-17 -0.40125342558848587 2313 18-19 -0.47391277840918633 2313 20-21 -0.410460798367005 2313 22-23 -0.4208408199961937 2313 24-25 -0.38446951004944196 2313 26-27 -0.529381612489459 2313 28-29 -0.5298074448905794 2313 30-31 -0.5577956954753134 2313 32-33 -0.5421249418425447 2313 34-35 -0.43105300793104817 2313 36-37 -0.5759393363472114 2313 38-39 -0.5884615703269986 2313 40-41 -0.6020976666384357 2313 42-43 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22-23 -0.783238796593487 2315 24-25 -0.6866965190394936 2315 26-27 -0.7503660648258048 2315 28-29 -0.560919435338576 2315 30-31 -0.5397397510068416 2315 32-33 -0.561796204981043 2315 34-35 -0.49098475419324217 2315 36-37 -0.48899276793815005 2315 38-39 -0.6108563464529837 2315 40-41 -0.6059091674966837 2315 42-43 -0.5606515851770766 2315 44-45 -0.6669667275844446 2315 46-47 -0.6265058828037908 2315 48-49 -0.5885535485929907 2315 50-51 -0.5791878421849859 2315 52-53 -0.5644046083164085 2315 54-55 -0.392449493106362 2315 56-57 0.31987819553020813 2315 58-59 3.3219224090426245 2315 60-61 3.406553857241761 2315 62-63 0.6253344710771351 2315 64-65 -0.047090622544224914 2315 66-67 -1.6199341042905822 2315 68-69 -4.692396767642151 2315 70-71 -3.426251845627162 2315 72-73 -3.2146094093616355 2315 74-75 -0.6827127832320947 2315 76-77 1.8043750256431679 2315 78-79 3.6848938107396734 2315 80-81 2.5293842784213325 2315 82-83 0.889247910752669 2315 84-85 0.5719249801581654 2315 86-87 0.49466872969245657 2315 88-89 0.5509025667350791 2315 90-91 2.198912345916245 2315 92-93 3.7759944776503325 2315 94-95 6.3878989988948796 2315 96-97 4.385512793383185 2315 98-99 6.324498546866021 2315 100-101 6.443035067930598 2316 1 -0.39875923094800214 2316 2 -0.4092860933692286 2316 3 -0.5179398686414203 2316 4 -0.38321103336125617 2316 5 -0.530393842072769 2316 6 -0.6150690027067043 2316 7 -0.5160708194543702 2316 8 -0.5928910666993517 2316 9 -0.8385362151262399 2316 10-11 -0.6741883943574791 2316 12-13 -0.6640486285155944 2316 14-15 -0.8291591164510876 2316 16-17 -0.9105984425539688 2316 18-19 -0.7905609066821739 2316 20-21 -0.7477999643107012 2316 22-23 -1.0637118870553408 2316 24-25 -0.8152690354157812 2316 26-27 -0.837707747910521 2316 28-29 -0.8923466683540866 2316 30-31 -0.7198588091826466 2316 32-33 -0.7230320311591925 2316 34-35 -0.6255280110974937 2316 36-37 -0.6793947627794736 2316 38-39 -0.836437853880625 2316 40-41 -0.9228573185864448 2316 42-43 -0.8516106049519934 2316 44-45 -0.7363387419732135 2316 46-47 -0.9861410936423951 2316 48-49 -0.8640917779110637 2316 50-51 -0.8247931381147211 2316 52-53 -0.7443076614954691 2316 54-55 -0.6364407757962738 2316 56-57 0.16544753882104146 2316 58-59 3.1804947519209144 2316 60-61 3.2280806202705037 2316 62-63 0.7125496934051689 2316 64-65 0.1785563321805128 2316 66-67 -1.8408782996261799 2316 68-69 -3.170993477349171 2316 70-71 -3.062563143430676 2316 72-73 -0.6620867485964688 2316 74-75 5.667195455609093 2316 76-77 5.613322316954001 2316 78-79 4.771977656534659 2316 80-81 3.075991525239573 2316 82-83 1.1753009857153671 2316 84-85 0.8516879819047105 2316 86-87 0.7098508640261691 2316 88-89 0.7909359747368079 2316 90-91 2.366254433829603 2316 92-93 3.783969987445257 2316 94-95 6.380831076359804 2316 96-97 3.8511168169943346 2316 98-99 6.147098973285527 2316 100-101 6.148693704853283 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 15.0 4 38.0 5 141.0 6 307.0 7 708.0 8 1605.0 9 2908.0 10 4629.0 11 7410.0 12 10816.0 13 14694.0 14 18368.0 15 23929.0 16 31758.0 17 41869.0 18 43599.0 19 40483.0 20 42457.0 21 43258.0 22 43052.0 23 44542.0 24 46956.0 25 49583.0 26 62232.0 27 88012.0 28 124141.0 29 164198.0 30 199469.0 31 222157.0 32 233038.0 33 232986.0 34 238179.0 35 255129.0 36 256822.0 37 200273.0 38 81336.0 39 5426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48298783929128 20.335134958447174 14.396726821680842 22.7851503805807 2 15.13028724212444 22.05535534543634 39.76169490759332 23.052662504845898 3 18.431592585910142 26.86434281943861 31.035524485637694 23.668540109013556 4 12.151172472100924 18.034860764483863 38.78323493349102 31.03073182992419 5 12.49249934516022 38.9879603401897 34.82404898653761 13.695491328112471 6 29.561773929621427 38.43170014972907 18.21177537233242 13.794750548317086 7 26.700165372919933 34.197777946963164 22.108346552390053 16.993710127726853 8 25.596413453997318 35.23160560230945 20.60873386248978 18.563247081203453 9 26.552904559627244 16.39142174474862 21.050091968480068 36.00558172714407 10-11 23.972103184080538 27.21815076434133 28.570457659213588 20.23928839236455 12-13 25.43840162918573 25.04514990114822 28.661348451553938 20.855100018112108 14-15 22.282298839705867 25.931962042918645 27.527870559900325 24.257868557475167 16-17 21.12250930244564 29.379267405137828 27.865289705977187 21.63293358643935 18-19 20.954667453731684 27.846509806416424 30.501011729667503 20.69781101018439 20-21 21.9971535138356 26.96313856039692 30.143516717543957 20.89619120822352 22-23 21.56771233663905 27.122358231048597 30.01405513380884 21.29587429850351 24-25 21.55636830361372 27.19687497772919 30.181241349149417 21.065515369507672 26-27 21.43959912714065 27.24586245269028 30.34611577936279 20.968422640806278 28-29 21.000594474515815 27.467156152115948 30.41933050349558 21.112918869872658 30-31 21.290357571038868 27.254863820331593 30.2602711651214 21.194507443508133 32-33 21.182686372369787 27.409691803021076 30.073615649787744 21.334006174821393 34-35 21.737046806624456 27.204422098323892 29.943324482763117 21.11520661228853 36-37 21.76292638305875 26.923285016893274 29.990917072928024 21.322871527119958 38-39 21.31417975838147 27.455330388904343 30.088981736572933 21.14150811614125 40-41 21.43706351614277 27.216864803778844 30.17971861839377 21.166353061684614 42-43 21.242544378821755 27.374507916824115 29.717213534000976 21.66573417035316 44-45 21.439092191158455 27.429000013570366 29.499159507138188 21.63274828813299 46-47 21.623629807157013 27.560813180573184 29.102036570022495 21.71352044224731 48-49 21.69327512332979 27.67842527525345 28.958943299520527 21.66935630189623 50-51 21.44450607913442 27.790907764179295 28.96041296054029 21.804173196145992 52-53 21.805605052070913 27.936453605256606 28.62664519025185 21.631296152420628 54-55 21.369836856194468 27.666779764123113 28.796722480928068 22.166660898754355 56-57 21.582323374107403 28.032060574246138 28.60702332585757 21.778592725788894 58-59 20.896983042303223 27.316927590214668 30.056852393821853 21.729236973660257 60-61 21.12272657434405 27.690942293046263 29.69609644049213 21.490234692117557 62-63 21.673154429379917 27.63162880796095 28.920617527006463 21.77459923565267 64-65 21.37747332276598 27.71788471944514 28.82594444335321 22.078697514435667 66-67 21.472184370955883 27.625210085510183 28.900649870368568 22.001955673165373 68-69 20.964600034382375 27.08234934842551 30.19043066594675 21.762619951245366 70-71 21.84100242721297 27.27691329394613 29.10136185115822 21.780722427682683 72-73 21.167532658506765 27.18869904095304 29.57885775431502 22.06491054622517 74-75 21.070016465474783 27.673034466952696 29.24044064243868 22.01650842513384 76-77 20.004051345286495 27.839657768121935 29.759162054336695 22.39712883225487 78-79 21.191416815509147 27.7588963348483 28.715135335521737 22.33455151412082 80-81 21.13941513846017 28.18795027202377 28.330778401131774 22.341856188384284 82-83 21.466172621221418 28.070099201344956 28.07880763011322 22.3849205473204 84-85 21.30781258012173 27.912945759273693 27.95966949216621 22.819572168438363 86-87 21.32609970613317 27.8093867037862 28.21583932823287 22.648674261847756 88-89 21.443594500036156 27.74805251330771 27.961817162913068 22.846535823743068 90-91 21.144685896363875 27.81358004432525 28.318512619129123 22.723221440181753 92-93 21.067633457660374 27.89556876447026 28.369138442654695 22.667659335214672 94-95 20.09385014363314 27.699494275200887 28.92249003410213 23.284165547063846 96-97 19.950733650573945 27.78133401839308 28.775389158046146 23.49254317298683 98-99 20.487411804975807 27.543678389505814 28.377236041971553 23.59167376354682 100-101 19.662815006530714 27.657041623692262 29.01584551691544 23.664297852861583 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1079.0 1 809.0 2 555.0 3 622.0 4 678.5 5 745.0 6 992.0 7 1441.0 8 2101.0 9 2720.0 10 2978.0 11 3132.0 12 3361.0 13 3530.0 14 3794.0 15 4146.5 16 4506.5 17 4970.5 18 5440.5 19 5887.5 20 6589.0 21 7553.5 22 8775.0 23 10256.0 24 12116.0 25 14966.5 26 19339.0 27 24152.0 28 28652.0 29 34027.0 30 40095.0 31 46880.5 32 55155.0 33 63886.0 34 72545.0 35 80282.0 36 87302.0 37 93020.5 38 95738.5 39 96234.5 40 96422.5 41 98474.5 42 100454.0 43 101641.5 44 102946.5 45 104037.0 46 105356.0 47 108553.5 48 112675.0 49 110368.5 50 102992.0 51 96283.0 52 89864.5 53 84482.0 54 79007.0 55 71502.0 56 63686.0 57 56879.0 58 50115.0 59 43078.5 60 35892.0 61 28874.5 62 22703.0 63 17247.0 64 12857.0 65 9410.0 66 6620.5 67 4710.0 68 3068.5 69 1850.5 70 1242.5 71 867.5 72 626.0 73 456.0 74 319.5 75 205.0 76 125.5 77 60.0 78 29.5 79 17.5 80 5.0 81 2.5 82 3.0 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027533183221862184 2 0.013349422168175602 3 2.0858472137774377E-4 4 0.0 5 0.06188013400873066 6 0.0 7 0.0 8 3.476412022962397E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 5.909900439036074E-4 16-17 1.7382060114811984E-5 18-19 6.779003444776674E-4 20-21 0.0 22-23 0.0 24-25 1.912026612629318E-4 26-27 2.0858472137774377E-4 28-29 0.0013558006889553347 30-31 0.005683933657543519 32-33 0.005927282499150886 34-35 0.002311813995269994 36-37 0.08536329722384164 38-39 0.07952292502526481 40-41 0.08430299155683812 42-43 0.08770987533934127 44-45 0.09108199500161479 46-47 0.08624978228969707 48-49 0.07729802133056889 50-51 0.07180529033428831 52-53 0.060750300101267876 54-55 0.04962578162778822 56-57 0.06632994139812252 58-59 0.695595281674546 60-61 0.3308675142854461 62-63 0.0030592425802069088 64-65 0.002068465153662626 66-67 1.0763493084895024 68-69 2.3274578493733244 70-71 2.305208812426365 72-73 1.7717012413224411 74-75 1.252898893075648 76-77 0.7191827372503459 78-79 0.5507505747378177 80-81 0.03632850563995705 82-83 1.912026612629318E-4 84-85 0.0016165315906775143 86-87 0.0019467907328589423 88-89 3.476412022962397E-5 90-91 0.3238103978788324 92-93 0.05042535639306956 94-95 0.9233350332988126 96-97 1.6201992053617398 98-99 0.6215303235253321 100-101 0.5497771793713883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2876529.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.976049750028764 #Duplication Level Percentage of deduplicated Percentage of total 1 70.97249259686403 31.210898653232412 2 14.559155141485622 12.805082616407176 3 5.655402773454729 7.461068211656875 4 2.7688910604070105 4.870595640994744 5 1.5790759605436993 3.472076149997209 6 0.9801070532343099 2.5860741920031662 7 0.6680419402479748 2.0564491919615477 8 0.479455563246281 1.6867649361797212 9 0.33691718069347326 1.333465802983405 >10 1.7224546802046135 13.795571041853313 >50 0.16115735336252907 4.940305272208323 >100 0.10765173162307101 8.967239576951888 >500 0.006897723474594372 2.1215293801863857 >1k 0.001982104446706917 1.3548598176469722 >5k 1.5856835573655335E-4 0.44802873611682226 >10k+ 1.5856835573655335E-4 0.8899907796200781 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14581 0.506895637068147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10945 0.3804932959132343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7500 0.2607309017221798 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5350 0.18598804322848822 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3009 0.10460523777093853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7382060114811984E-5 0.0 18-19 0.0 0.0 0.0 6.952824045924793E-5 0.0 20-21 0.0 0.0 0.0 1.2167442080368389E-4 1.7382060114811984E-5 22-23 0.0 0.0 0.0 4.171694427554876E-4 3.476412022962397E-5 24-25 0.0 0.0 0.0 9.73395366429471E-4 3.476412022962397E-5 26-27 0.0 0.0 0.0 0.0016512957109071384 3.476412022962397E-5 28-29 0.0 0.0 0.0 0.0044324253292770555 3.476412022962397E-5 30-31 0.0 0.0 0.0 0.01279319624450162 3.476412022962397E-5 32-33 0.0 0.0 0.0 0.02984499721713218 3.476412022962397E-5 34-35 0.0 0.0 0.0 0.05383224017557271 3.476412022962397E-5 36-37 0.0 0.0 0.0 0.08960451989185578 3.476412022962397E-5 38-39 0.0 0.0 0.0 0.14581810230315773 3.476412022962397E-5 40-41 0.0 0.0 0.0 0.20992314000658432 3.476412022962397E-5 42-43 0.0 0.0 0.0 0.27244641023956306 3.476412022962397E-5 44-45 0.0 0.0 0.0 0.34565964744315114 3.476412022962397E-5 46-47 0.0 0.0 0.0 0.4352467852748921 3.476412022962397E-5 48-49 0.0 0.0 0.0 0.5261723417354736 3.476412022962397E-5 50-51 0.0 0.0 0.0 0.62264277537268 3.476412022962397E-5 52-53 1.7382060114811984E-5 0.0 0.0 0.7173576209382906 3.476412022962397E-5 54-55 3.476412022962397E-5 0.0 0.0 0.8211111377636033 3.476412022962397E-5 56-57 3.476412022962397E-5 0.0 0.0 0.9198064750955057 3.476412022962397E-5 58-59 3.476412022962397E-5 1.7382060114811984E-5 0.0 0.9954705827752823 3.476412022962397E-5 60-61 3.476412022962397E-5 5.214618034443595E-5 0.0 1.063260617223049 5.214618034443595E-5 62-63 3.476412022962397E-5 6.952824045924793E-5 0.0 1.1207257079626174 6.952824045924793E-5 64-65 3.476412022962397E-5 6.952824045924793E-5 0.0 1.1831099217146777 6.952824045924793E-5 66-67 3.476412022962397E-5 6.952824045924793E-5 3.476412022962397E-5 1.238263198458976 6.952824045924793E-5 68-69 3.476412022962397E-5 6.952824045924793E-5 5.214618034443595E-5 1.3063834920489241 6.952824045924793E-5 70-71 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 1.4126574075908847 6.952824045924793E-5 72-73 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 1.5472466990598739 6.952824045924793E-5 74-75 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 1.7359637257263876 6.952824045924793E-5 76-77 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 1.971368965861286 6.952824045924793E-5 78-79 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 2.2696798815516894 6.952824045924793E-5 80-81 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 2.6283760740809496 6.952824045924793E-5 82-83 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 2.987141794850669 8.691030057405991E-5 84-85 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 3.3059461594164357 1.042923606888719E-4 86-87 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 3.593793074917722 1.042923606888719E-4 88-89 3.476412022962397E-5 1.042923606888719E-4 6.952824045924793E-5 3.8750174255152654 1.042923606888719E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14250 0.0 27.195206 1 TACATGG 14195 0.0 26.727837 2 GGTATCA 13825 0.0 26.153372 1 GTATCAA 19660 0.0 26.122183 1 ACATGGG 13930 0.0 25.536724 3 CATGGGA 9495 0.0 22.906086 4 CATTCCG 545 0.0 22.493523 9 ATCAACG 23210 0.0 21.932657 3 TCAACGC 23370 0.0 21.782497 4 CAACGCA 23565 0.0 21.602247 5 AACGCAG 23705 0.0 21.461601 6 TATCAAC 24125 0.0 21.20338 2 CATGGGG 4705 0.0 21.057394 4 GAGTACT 11160 0.0 20.87137 12-13 AGTACTT 11755 0.0 20.436653 12-13 ATGGGAG 4060 0.0 19.405972 5 AGAGTAC 19865 0.0 19.21361 10-11 AGTACAT 10455 0.0 19.182627 2 ACGCAGA 26270 0.0 19.060974 7 CGCAGAG 25990 0.0 18.994204 8 >>END_MODULE