FastQCFastQC Report
Fri 27 May 2016
SRR939667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855659
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132150.4627653371778633No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA94910.3323576099247144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89060.31187197070798717No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA72580.2541619990341984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65490.22933410466725893No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37280.13054779999992996No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA32680.11443943412011028No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30870.10810114232826819No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT30230.10585997837977154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG138050.028.8759231
TACATGG138750.028.2861022
ACATGGG141450.026.8067683
CATGGGA90450.024.8172234
ACCGTAT2252.8503564E-923.1991848
CATGGGG54300.022.1116584
GAGTACT100750.021.80712712-13
AGTACTT110000.020.94397412-13
GTATCAA253550.020.7568241
GTACCGT3153.255991E-1019.5823556
ATGGGAT39600.019.5344245
TACCGTA2702.3295797E-819.3319767
GTACTTT113800.019.28588714-15
ATGGGAG39550.018.7191585
TATCAAC286950.018.3411222
ATCAACG290200.018.2828963
TCAACGC291200.018.1386264
CAACGCA291900.018.0954465
AACGCAG298250.017.7388946
TACTTTT130850.017.46194814-15
Produced by FastQC (version 0.11.5)