##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939667_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2855659 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.190692236012772 31.0 30.0 33.0 26.0 34.0 2 30.33467931570261 31.0 30.0 34.0 26.0 34.0 3 30.37043883741021 31.0 30.0 34.0 26.0 34.0 4 33.96832359886107 35.0 35.0 37.0 28.0 37.0 5 33.9603187915644 35.0 35.0 37.0 28.0 37.0 6 34.07289385742485 36.0 35.0 37.0 29.0 37.0 7 33.88327878083483 36.0 35.0 37.0 28.0 37.0 8 33.89461206677688 35.0 35.0 37.0 28.0 37.0 9 35.14391038986098 38.0 35.0 39.0 28.0 39.0 10-11 35.044659919129 37.0 34.5 39.0 28.0 39.0 12-13 34.89031918726991 37.0 34.0 39.0 27.0 39.0 14-15 35.54036441325803 38.0 34.0 40.0 27.0 41.0 16-17 35.281990601819054 38.0 33.5 40.0 27.0 41.0 18-19 35.28970703434828 38.0 34.0 40.0 27.0 41.0 20-21 35.408422714336695 38.0 34.0 40.0 27.0 41.0 22-23 35.25235838732846 38.0 34.0 40.0 26.0 41.0 24-25 35.12746427357048 38.0 34.0 40.0 25.0 41.0 26-27 35.23620449780593 38.0 34.0 40.0 26.0 41.0 28-29 35.10258402701443 38.0 34.0 40.0 25.0 41.0 30-31 34.87133075062533 38.0 34.0 40.0 25.0 41.0 32-33 34.627500342302774 38.0 33.0 40.0 24.0 41.0 34-35 34.39464936114571 38.0 33.0 40.0 24.0 41.0 36-37 34.08525107514588 38.0 33.0 40.0 23.0 41.0 38-39 33.91498547270525 38.0 33.0 40.0 22.5 41.0 40-41 33.610418470832826 38.0 32.5 40.0 20.0 41.0 42-43 33.28937068466509 37.0 31.5 40.0 18.5 41.0 44-45 33.03104712432402 37.0 31.0 40.0 17.0 41.0 46-47 32.53977470699408 37.0 31.0 40.0 14.0 41.0 48-49 32.16010227411606 37.0 30.0 39.5 10.5 41.0 50-51 31.53295614077171 36.0 30.0 38.5 9.0 40.5 52-53 31.557857748421643 36.0 30.0 39.0 8.0 40.0 54-55 31.602061555668936 36.0 30.0 39.0 8.0 40.0 56-57 31.483133315287294 36.0 30.0 39.0 7.0 40.0 58-59 31.09684174476014 36.0 29.0 39.0 2.0 40.0 60-61 30.671788543380003 35.0 28.0 39.0 2.0 40.0 62-63 30.232824892607976 35.0 28.0 38.0 2.0 40.0 64-65 29.59802010674244 34.0 27.5 38.0 2.0 40.0 66-67 29.070311791428878 34.0 26.0 38.0 2.0 40.0 68-69 28.56173741332561 34.0 26.0 37.0 2.0 39.0 70-71 28.010219882696077 33.5 26.0 36.5 2.0 39.0 72-73 27.256175194587307 32.5 24.0 36.0 2.0 38.5 74-75 26.795041529818512 32.0 23.0 35.0 2.0 38.0 76-77 26.414085680398117 32.0 22.5 35.0 2.0 37.5 78-79 25.840172443558565 31.5 20.5 35.0 2.0 37.0 80-81 25.184523257153604 31.0 19.0 34.5 2.0 36.0 82-83 24.52944259100964 31.0 17.0 34.0 2.0 36.0 84-85 23.866265720101733 30.0 9.0 34.0 2.0 35.0 86-87 23.174353275373566 30.0 4.5 34.0 2.0 35.0 88-89 22.355905239386075 29.0 2.0 34.0 2.0 35.0 90-91 21.11865912561689 28.0 2.0 33.5 2.0 35.0 92-93 19.50829983551958 25.5 2.0 32.5 2.0 34.0 94-95 19.242692842527767 25.0 2.0 32.0 2.0 34.0 96-97 18.60287852996454 25.0 2.0 32.0 2.0 34.0 98-99 17.59832458987575 23.5 2.0 32.0 2.0 34.0 100-101 15.981376627951727 10.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9268292179526902 1101 2 0.8693602843821573 1101 3 0.8121912973356196 1101 4 0.9571497193567069 1101 5 0.8584894793693181 1101 6 0.6936245546385535 1101 7 0.7817173339930008 1101 8 0.8448297660943709 1101 9 1.108650373069409 1101 10-11 0.8468245730741017 1101 12-13 0.8595239862768693 1101 14-15 0.8781478240903624 1101 16-17 0.7172355796187233 1101 18-19 0.7673532713556313 1101 20-21 1.0415585995303616 1101 22-23 1.0763409283338135 1101 24-25 0.8164147311513759 1101 26-27 0.8372706848806573 1101 28-29 1.0556944433320368 1101 30-31 1.206587731946719 1101 32-33 1.027455224710046 1101 34-35 0.9917629933913403 1101 36-37 0.9519542319389913 1101 38-39 0.8252596285274052 1101 40-41 1.1130004390645638 1101 42-43 0.9869561776664924 1101 44-45 0.8435721736751844 1101 46-47 0.8069962597231211 1101 48-49 0.7761406511620592 1101 50-51 0.7071538327307394 1101 52-53 0.9446970593387611 1101 54-55 0.8905679481494957 1101 56-57 0.7055561905930894 1101 58-59 0.6598953085199071 1101 60-61 0.46978833412411447 1101 62-63 0.40078844724863316 1101 64-65 0.39935406961560105 1101 66-67 0.3872437573161882 1101 68-69 0.4214753720898621 1101 70-71 0.3013590447813961 1101 72-73 0.4867927017376239 1101 74-75 0.20816640011808474 1101 76-77 -0.28880825993509873 1101 78-79 -0.09463778465016759 1101 80-81 -0.383416867797699 1101 82-83 -0.4663516152793612 1101 84-85 -0.6812186950688144 1101 86-87 -1.2398080521737675 1101 88-89 -1.9731245076750135 1101 90-91 -5.349007178954466 1101 92-93 -9.8481274167851 1101 94-95 -8.184320598160419 1101 96-97 -5.434954854103889 1101 98-99 -4.2906372984107914 1101 100-101 -3.737084680111634 1102 1 0.745174616427267 1102 2 0.6363978769483118 1102 3 0.6094544353012452 1102 4 0.675044576891608 1102 5 0.6751539018187032 1102 6 0.5870885399498178 1102 7 0.5523682205317328 1102 8 0.5533207804253877 1102 9 0.8094683512181291 1102 10-11 0.5682300164934233 1102 12-13 0.5279997732551394 1102 14-15 0.714459327489962 1102 16-17 0.8409286094339024 1102 18-19 0.6648768426665939 1102 20-21 0.698189683637267 1102 22-23 0.8489027320471862 1102 24-25 0.8196007907192566 1102 26-27 0.679994140971111 1102 28-29 0.6853370441788869 1102 30-31 0.8551565826064973 1102 32-33 0.8956344313443481 1102 34-35 0.8008567753265226 1102 36-37 0.573671829841274 1102 38-39 0.44958171609312103 1102 40-41 0.45617393622264046 1102 42-43 0.5566514650288781 1102 44-45 0.29725152886521755 1102 46-47 0.44634817246794967 1102 48-49 0.43770281697025126 1102 50-51 0.47830170144808903 1102 52-53 0.7332402415680583 1102 54-55 0.5636360754006802 1102 56-57 0.4946700861304443 1102 58-59 0.48427916152752815 1102 60-61 0.5274887226809177 1102 62-63 0.45326689104692264 1102 64-65 0.37468483652244444 1102 66-67 0.388745013713379 1102 68-69 0.17092027978379676 1102 70-71 0.2118749158756401 1102 72-73 0.11671648062046458 1102 74-75 -0.00825016389256561 1102 76-77 -0.25957121154217333 1102 78-79 -0.2580950123159589 1102 80-81 -0.5120511329654605 1102 82-83 -0.6828724494783565 1102 84-85 -1.060168660686987 1102 86-87 -1.5335477169283216 1102 88-89 -2.0627910898841293 1102 90-91 -5.699108824814479 1102 92-93 -10.660553470866883 1102 94-95 -9.293818354828321 1102 96-97 -6.004522439774934 1102 98-99 -4.759968038449513 1102 100-101 -4.051737273713458 1103 1 0.6664285569403425 1103 2 0.7143509156561265 1103 3 0.6372053773291135 1103 4 0.6726917004566317 1103 5 0.6686179271740968 1103 6 0.4606006622946026 1103 7 0.5730596027911403 1103 8 0.5022701010009172 1103 9 0.4694470675293658 1103 10-11 0.5512107750392019 1103 12-13 0.5463045165455682 1103 14-15 0.4433166704157543 1103 16-17 0.6629942730431537 1103 18-19 0.6331021775404224 1103 20-21 0.7633999577624593 1103 22-23 0.769514253950554 1103 24-25 0.5503243776508597 1103 26-27 0.3949597962327758 1103 28-29 0.6103594949347624 1103 30-31 0.5110956914776423 1103 32-33 0.747634635514629 1103 34-35 0.5503016873658169 1103 36-37 0.5981223512464595 1103 38-39 0.5728772398191495 1103 40-41 0.27148317179101156 1103 42-43 0.3608230520753395 1103 44-45 0.2715180311677159 1103 46-47 0.3620364788807606 1103 48-49 0.37767429427518806 1103 50-51 0.23805030083462242 1103 52-53 0.3261314071092194 1103 54-55 0.40462178454415465 1103 56-57 0.258302506776527 1103 58-59 0.18684399755592906 1103 60-61 0.05994109756946031 1103 62-63 0.21865296961101066 1103 64-65 0.005530074915178318 1103 66-67 0.015374986442825644 1103 68-69 -0.07455667050765058 1103 70-71 0.05750416975553918 1103 72-73 0.08522680318293041 1103 74-75 -0.10292403110637949 1103 76-77 -0.34081948686154817 1103 78-79 -0.1934466052429471 1103 80-81 -0.40497704467350815 1103 82-83 -0.6094496383406529 1103 84-85 -0.9005873852288424 1103 86-87 -1.2220415516769343 1103 88-89 -1.5447268049559888 1103 90-91 -3.119008542113068 1103 92-93 -9.363727959896186 1103 94-95 -5.911188753220907 1103 96-97 -3.4985768442437113 1103 98-99 -2.617282385718708 1103 100-101 -2.1195751768633695 1104 1 0.8583909321186596 1104 2 0.7814688226144391 1104 3 0.8818697456917697 1104 4 0.8405441731147434 1104 5 0.8494870229103455 1104 6 0.748984815884711 1104 7 0.8705433561593452 1104 8 0.7299943837715617 1104 9 0.8409976302175863 1104 10-11 0.6020419289463206 1104 12-13 0.7259073161176062 1104 14-15 0.7665327605251235 1104 16-17 0.915919162090546 1104 18-19 0.8097389761614409 1104 20-21 0.8091899502335309 1104 22-23 0.777810437973379 1104 24-25 0.6870578635440339 1104 26-27 0.6477625878796331 1104 28-29 0.780572930106743 1104 30-31 0.9265663980079992 1104 32-33 0.8351408270663825 1104 34-35 0.7634597254859656 1104 36-37 0.864663205581536 1104 38-39 0.754983655257405 1104 40-41 0.7353019723140761 1104 42-43 0.9002830496977836 1104 44-45 0.7585653049036551 1104 46-47 0.6428493553271082 1104 48-49 0.7524065715774526 1104 50-51 0.834639251143706 1104 52-53 0.870819055706459 1104 54-55 0.7963062828798684 1104 56-57 0.8709352536460848 1104 58-59 0.8279140462958203 1104 60-61 0.8697646135783366 1104 62-63 0.7207789008203918 1104 64-65 0.9080765363195198 1104 66-67 0.7831761930524301 1104 68-69 0.7217796644899686 1104 70-71 0.8096766614340929 1104 72-73 0.9715187076051812 1104 74-75 0.6632210592407439 1104 76-77 0.742207736153194 1104 78-79 0.46158762165513423 1104 80-81 0.4426907216127489 1104 82-83 0.48368913167043814 1104 84-85 0.36573750778114444 1104 86-87 0.03224448810750147 1104 88-89 -0.14940439847318743 1104 90-91 -0.6500358939120865 1104 92-93 -4.149553268978451 1104 94-95 -1.8033009639516102 1104 96-97 -0.9159570253342153 1104 98-99 -0.7512549348548632 1104 100-101 -0.704604995376604 1105 1 0.9750648225486174 1105 2 1.056529020641868 1105 3 0.9053575712427033 1105 4 0.7855886714966189 1105 5 0.7806527456719436 1105 6 0.8115556087649765 1105 7 0.9072092522912598 1105 8 0.8143351912919812 1105 9 0.9830669012917141 1105 10-11 0.8491225654080239 1105 12-13 0.9538091018318937 1105 14-15 1.0359191740711253 1105 16-17 1.22532498348059 1105 18-19 0.9942924031147484 1105 20-21 0.8850421135098472 1105 22-23 0.9217034684865624 1105 24-25 1.011386294350011 1105 26-27 1.0465391231701204 1105 28-29 0.9326329066054342 1105 30-31 1.090181987852354 1105 32-33 1.2904909869523493 1105 34-35 1.1096655642130386 1105 36-37 1.0646391263705652 1105 38-39 1.2736734572299753 1105 40-41 1.2032840862761631 1105 42-43 1.318546168051924 1105 44-45 1.2640890759007277 1105 46-47 1.167411354479519 1105 48-49 1.077991455611972 1105 50-51 1.0806943540100917 1105 52-53 1.0019838152996137 1105 54-55 1.1275765094170822 1105 56-57 1.2140774943611383 1105 58-59 1.1584958481913397 1105 60-61 0.8572499252596479 1105 62-63 0.9602938491945636 1105 64-65 1.0417676867279049 1105 66-67 1.0288860000334772 1105 68-69 0.8515870789606161 1105 70-71 0.822069749737377 1105 72-73 1.121687743666211 1105 74-75 1.0379248849457845 1105 76-77 0.6790518184463039 1105 78-79 0.5575839712467499 1105 80-81 0.5883557412613847 1105 82-83 0.6686964517505736 1105 84-85 0.6416477019559004 1105 86-87 0.1315312618784965 1105 88-89 -0.1272315386334597 1105 90-91 -1.2655917865765964 1105 92-93 -7.394396946740818 1105 94-95 -3.0163453949116956 1105 96-97 -1.2878698296954418 1105 98-99 -0.783942704542028 1105 100-101 -0.6337133903527175 1106 1 0.8364387716248309 1106 2 0.7907005245544738 1106 3 0.7588318268205185 1106 4 0.7584564306103303 1106 5 0.6211195185011746 1106 6 0.7404806271721895 1106 7 0.8495997935491175 1106 8 0.8831028493800233 1106 9 0.9369632386831981 1106 10-11 0.8303818760362631 1106 12-13 0.8649643046537605 1106 14-15 0.8820117067332376 1106 16-17 0.9565551850182388 1106 18-19 0.7776098976781967 1106 20-21 0.8656190728084852 1106 22-23 0.8810787360333521 1106 24-25 0.8058988026974774 1106 26-27 0.8518879185674635 1106 28-29 0.9387877669674154 1106 30-31 0.8810169050626726 1106 32-33 0.9355073394120623 1106 34-35 0.8360264141984857 1106 36-37 0.9537394842411473 1106 38-39 0.9503485141639345 1106 40-41 1.0374872677285367 1106 42-43 1.1670654571051955 1106 44-45 0.9667332943216422 1106 46-47 1.1229502151154662 1106 48-49 0.9247693361277172 1106 50-51 0.735209687651647 1106 52-53 0.8570569304859994 1106 54-55 0.9574394398463539 1106 56-57 0.9048417792192716 1106 58-59 0.775056352292296 1106 60-61 0.7648842123437696 1106 62-63 0.7778115286863567 1106 64-65 0.7138580618927115 1106 66-67 0.6149470263857637 1106 68-69 0.5929960445605182 1106 70-71 0.5484002064076385 1106 72-73 0.8092226317674402 1106 74-75 0.6863196042142938 1106 76-77 0.4419762546717152 1106 78-79 0.4753525026075138 1106 80-81 0.5071169782265059 1106 82-83 0.5482173503653236 1106 84-85 0.41771061183758107 1106 86-87 0.3738725745272511 1106 88-89 0.2263809851246954 1106 90-91 -0.11217489303025374 1106 92-93 -3.6119595740930883 1106 94-95 -1.1179476791191583 1106 96-97 -0.2746127793024691 1106 98-99 0.1610218508594201 1106 100-101 0.18897414924420453 1107 1 0.5297248431771635 1107 2 0.5718026115521759 1107 3 0.5419047849582945 1107 4 0.487072890283045 1107 5 0.42414966428862044 1107 6 0.5013496141036313 1107 7 0.3691333309836793 1107 8 0.21117416432993252 1107 9 0.3167364608642629 1107 10-11 0.2812102395464038 1107 12-13 0.2651103724246795 1107 14-15 0.47786198637339083 1107 16-17 0.4931623092366735 1107 18-19 0.3654043816963295 1107 20-21 0.4544671056650884 1107 22-23 0.4217517213687145 1107 24-25 0.44627040769538695 1107 26-27 0.23328065001091858 1107 28-29 0.34543215069442823 1107 30-31 0.4663268651669057 1107 32-33 0.20994631451613088 1107 34-35 0.2541376249148115 1107 36-37 0.41042350130167193 1107 38-39 0.17512289641774714 1107 40-41 0.29096244604871657 1107 42-43 0.2072964219218818 1107 44-45 0.09143246568973495 1107 46-47 -0.007266130995368769 1107 48-49 -0.1558632941836393 1107 50-51 -0.1775805169207736 1107 52-53 0.08683155429737255 1107 54-55 0.19967258882057237 1107 56-57 -0.04896573459274833 1107 58-59 -0.10731256992557192 1107 60-61 -0.046671961121997896 1107 62-63 -0.2645435919462997 1107 64-65 -0.23188572366619908 1107 66-67 -0.3712213875472763 1107 68-69 -0.3446666563870444 1107 70-71 -0.25621712167942334 1107 72-73 -0.12272905194841854 1107 74-75 -0.09813088811580783 1107 76-77 -0.4625528601719111 1107 78-79 -0.39965541511261193 1107 80-81 -0.5888835442535552 1107 82-83 -0.44493738544525385 1107 84-85 -0.5049232125435097 1107 86-87 -0.6144485049785864 1107 88-89 -0.8026946724934056 1107 90-91 -1.0867763379619895 1107 92-93 -4.449895324552793 1107 94-95 -2.186404675013872 1107 96-97 -1.4480784861804175 1107 98-99 -1.0135061725018772 1107 100-101 -0.9990540193498507 1108 1 0.2637547691729729 1108 2 0.42726247010231333 1108 3 0.12540569667107349 1108 4 -0.2969509673939754 1108 5 -1.0073677523497508 1108 6 -0.24910297429633488 1108 7 -0.04497832179985295 1108 8 0.020389714957460114 1108 9 0.23745390405223787 1108 10-11 0.16025146095416432 1108 12-13 0.116570324281561 1108 14-15 0.1730356276249836 1108 16-17 0.32078362856468345 1108 18-19 0.1910703726403895 1108 20-21 0.104231128637025 1108 22-23 0.08870576453119128 1108 24-25 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0.6199327629112936 2311 72-73 0.6484193980354966 2311 74-75 0.6660409853807572 2311 76-77 0.7599381472000815 2311 78-79 0.9190950477951461 2311 80-81 0.7558660989409418 2311 82-83 0.7871011419466107 2311 84-85 0.8616952791363133 2311 86-87 1.171717432873077 2311 88-89 1.340117719381663 2311 90-91 2.0358514294662022 2311 92-93 3.0166939211307344 2311 94-95 2.196673947737537 2311 96-97 2.0004216275238456 2311 98-99 1.7974334482536776 2311 100-101 1.963878064995905 2312 1 0.3342170139624976 2312 2 0.021320330401621845 2312 3 0.02410215919652714 2312 4 -0.0022690128369688978 2312 5 0.0035259719016735858 2312 6 0.07513231510076679 2312 7 0.14821922111178765 2312 8 -0.5435383328585246 2312 9 -0.05553080084752793 2312 10-11 -0.006464525532621224 2312 12-13 0.10675113471277342 2312 14-15 -0.1826034302870454 2312 16-17 0.11859666026133908 2312 18-19 0.0598874357098893 2312 20-21 0.3287972265479482 2312 22-23 0.20095542856603998 2312 24-25 0.3479411406946582 2312 26-27 0.34265927048650724 2312 28-29 0.19109820579399894 2312 30-31 0.3606769799563594 2312 32-33 0.4267469013320948 2312 34-35 0.2200221601572352 2312 36-37 0.31862539331018525 2312 38-39 0.41510666926389206 2312 40-41 0.46465041618363045 2312 42-43 0.48402305440146165 2312 44-45 0.3317106221399868 2312 46-47 0.7335182889008465 2312 48-49 0.5189417641665628 2312 50-51 0.5025587596103094 2312 52-53 0.4564072103003731 2312 54-55 0.47535535782499494 2312 56-57 0.3844008123374145 2312 58-59 0.42390465855671877 2312 60-61 0.4005705415076797 2312 62-63 0.34199114081203064 2312 64-65 0.49334392682856176 2312 66-67 0.4389670953729059 2312 68-69 0.2143052406894057 2312 70-71 0.2176944569812882 2312 72-73 0.2240694680316473 2312 74-75 0.3611328262523479 2312 76-77 0.6319243401042769 2312 78-79 0.7050199606851351 2312 80-81 0.6886342971850254 2312 82-83 0.766409681477068 2312 84-85 0.8395557109796385 2312 86-87 0.8262633158486956 2312 88-89 0.9279299605212721 2312 90-91 1.4930475896773352 2312 92-93 2.530878488625813 2312 94-95 1.9012441194078527 2312 96-97 1.843503065342066 2312 98-99 2.031992001227504 2312 100-101 1.956886002323822 2313 1 -0.24615657652642042 2313 2 -0.998682313471349 2313 3 -0.4085730745254921 2313 4 -0.001878938066226965 2313 5 -0.9416898650354426 2313 6 -0.3823650609883842 2313 7 -1.2077278024560485 2313 8 -1.074712818715426 2313 9 -1.3317419869538085 2313 10-11 -1.990126632652057 2313 12-13 -0.5600174127412387 2313 14-15 -0.21244119289623598 2313 16-17 -1.563588614115396 2313 18-19 -1.1414652769560334 2313 20-21 -1.563016171618436 2313 22-23 -1.2930998496720107 2313 24-25 -0.41422148824960203 2313 26-27 -0.9170338706938495 2313 28-29 -2.4457448442653984 2313 30-31 -0.7238127520670972 2313 32-33 -0.43044482253716865 2313 34-35 0.04342273283864273 2313 36-37 -0.10304370873610935 2313 38-39 -0.5114955921839979 2313 40-41 -0.1747667626225251 2313 42-43 0.11955535662433192 2313 44-45 -0.4912305751480446 2313 46-47 -0.5462516764064489 2313 48-49 -0.038223067512284103 2313 50-51 -0.0714439053584428 2313 52-53 0.037867874212171415 2313 54-55 0.23475986141911065 2313 56-57 -0.14770755609229624 2313 58-59 0.09931242075878188 2313 60-61 -0.08247530267790637 2313 62-63 -0.09625234372185076 2313 64-65 0.1247653614618578 2313 66-67 0.054494338551748456 2313 68-69 -0.08757748865893333 2313 70-71 0.03403548465530548 2313 72-73 0.21301803957625864 2313 74-75 0.2111144758812138 2313 76-77 0.38212901352927986 2313 78-79 0.5350232660192411 2313 80-81 0.5475691337996906 2313 82-83 0.35887731498106845 2313 84-85 0.5691503496547661 2313 86-87 0.700411709281834 2313 88-89 0.6456997245314575 2313 90-91 1.3137552466272275 2313 92-93 2.414311933264031 2313 94-95 1.6808658227856164 2313 96-97 1.2087242964898088 2313 98-99 1.101564158110314 2313 100-101 1.112148231246751 2314 1 -0.37481453490563865 2314 2 -0.4768087404093002 2314 3 -1.7634806101863525 2314 4 -0.3801162613020068 2314 5 -0.22220105341937568 2314 6 -2.674551460017252 2314 7 -3.47032565264119 2314 8 -1.3345339462308914 2314 9 -1.5984684649403675 2314 10-11 -0.8764780650931598 2314 12-13 -1.8646047736583498 2314 14-15 -2.5828984151875147 2314 16-17 -1.853343508397863 2314 18-19 -2.3340088872549885 2314 20-21 -1.8964776389989595 2314 22-23 -2.9052214727240013 2314 24-25 -1.374873093031816 2314 26-27 -1.49623618714579 2314 28-29 -1.0263187571587835 2314 30-31 -1.2207854984016606 2314 32-33 -2.2844607932309415 2314 34-35 -0.5488818934088826 2314 36-37 -0.31945398000101477 2314 38-39 -0.4833800723468116 2314 40-41 -0.5453575088576912 2314 42-43 -0.6077028422765522 2314 44-45 -1.1920160649641645 2314 46-47 -1.3876147654084932 2314 48-49 -1.7259630398492192 2314 50-51 -0.8937038447330146 2314 52-53 -0.40212357866821336 2314 54-55 -0.01559941818251076 2314 56-57 -0.22978088533922758 2314 58-59 -0.3845742846708333 2314 60-61 -0.29915242342947934 2314 62-63 -0.4894639903825784 2314 64-65 -0.5568678248529473 2314 66-67 -0.4008939329073904 2314 68-69 -0.2626503936251048 2314 70-71 -0.42605687701125916 2314 72-73 -0.3866552037233113 2314 74-75 -0.0630612196064213 2314 76-77 0.03883587857456661 2314 78-79 0.18517183372694035 2314 80-81 0.12013612368490456 2314 82-83 0.14392588704367526 2314 84-85 -0.045944016358962614 2314 86-87 0.13445678343375178 2314 88-89 0.21029475960331823 2314 90-91 0.9203677669868959 2314 92-93 1.7146129375009878 2314 94-95 1.0952143296040155 2314 96-97 0.9492688718117464 2314 98-99 0.668161953139947 2314 100-101 0.9104905701715875 2315 1 -7.079093364363754 2315 2 -2.872804279004768 2315 3 -1.3448401928900253 2315 4 -0.9076865639036811 2315 5 -1.2168987252750227 2315 6 -1.6073227636797753 2315 7 -2.9138229196642165 2315 8 -2.7256728412423996 2315 9 -3.225173503227417 2315 10-11 -2.0455327500039857 2315 12-13 -2.6404793044854102 2315 14-15 -4.432056540144895 2315 16-17 -4.2355238166922575 2315 18-19 -2.974916069902214 2315 20-21 -4.151525955684949 2315 22-23 -5.164384913785412 2315 24-25 -3.416239002731789 2315 26-27 -4.479298935012611 2315 28-29 -2.7767091399658383 2315 30-31 -2.510644831751911 2315 32-33 -1.559341131336744 2315 34-35 -2.175836582442571 2315 36-37 -2.757032076383844 2315 38-39 -1.774851283324388 2315 40-41 -3.3024833082554395 2315 42-43 -7.442812637069384 2315 44-45 -4.05276565545369 2315 46-47 -2.6305183512613937 2315 48-49 -5.112229979232154 2315 50-51 -6.183626557063999 2315 52-53 -6.48997972988818 2315 54-55 -4.058551338560669 2315 56-57 -3.4403747686878106 2315 58-59 -3.7960678423138923 2315 60-61 -2.748646015958233 2315 62-63 -2.993308899291275 2315 64-65 -1.7486567830551287 2315 66-67 -2.1462652851720243 2315 68-69 -1.628657416749558 2315 70-71 -1.2734368226530073 2315 72-73 -1.685312322545574 2315 74-75 -2.423593220491245 2315 76-77 -2.33026923202101 2315 78-79 -1.5035933177641851 2315 80-81 -1.1933200614693291 2315 82-83 -1.5063722865316826 2315 84-85 -1.450028823877151 2315 86-87 -1.8659740350639105 2315 88-89 -1.4380551173453249 2315 90-91 -0.41872395225938064 2315 92-93 0.25691134576191743 2315 94-95 0.13563416307239962 2315 96-97 0.06870500007058666 2315 98-99 -0.16634165316865435 2315 100-101 0.04878870925767664 2316 1 -4.105407328889637 2316 2 -2.879388131579706 2316 3 -4.491480782564629 2316 4 -4.197656475683388 2316 5 -3.8085846196479736 2316 6 -4.141940652100821 2316 7 -2.9736582321551985 2316 8 -1.3699834578224284 2316 9 -2.0512442686691728 2316 10-11 -2.868406291290512 2316 12-13 -3.8166690639079164 2316 14-15 -2.885649288506997 2316 16-17 -2.7721868703139094 2316 18-19 -3.0267296608306893 2316 20-21 -5.655835554091791 2316 22-23 -6.62456472156714 2316 24-25 -4.346744674036621 2316 26-27 -3.8359508729088567 2316 28-29 -4.489018092193476 2316 30-31 -6.106354013429282 2316 32-33 -4.527384322724441 2316 34-35 -6.1346544833703085 2316 36-37 -9.371013157603603 2316 38-39 -6.168041809182782 2316 40-41 -5.490707453871842 2316 42-43 -4.569261329157776 2316 44-45 -3.3249253531123735 2316 46-47 -5.5876429379792825 2316 48-49 -3.1243764045623763 2316 50-51 -4.265933432859697 2316 52-53 -5.559947669458399 2316 54-55 -7.161545769874866 2316 56-57 -5.234756797690512 2316 58-59 -5.89176760388499 2316 60-61 -6.455096564007992 2316 62-63 -6.747047979985108 2316 64-65 -5.338287490837821 2316 66-67 -6.741689859383225 2316 68-69 -5.468344444742105 2316 70-71 -6.262644387278943 2316 72-73 -7.94797515008467 2316 74-75 -7.6118234791553405 2316 76-77 -7.150739243212897 2316 78-79 -6.141492950740812 2316 80-81 -6.264802032588484 2316 82-83 -5.260047130117833 2316 84-85 -6.859521745569207 2316 86-87 -5.618510448785361 2316 88-89 -5.372408701635635 2316 90-91 -5.676667306554787 2316 92-93 -4.541529045408996 2316 94-95 -4.411366956116396 2316 96-97 -4.9510174956242246 2316 98-99 -5.835250472151891 2316 100-101 -4.99068885164832 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14318.0 3 5673.0 4 6056.0 5 7034.0 6 8072.0 7 9443.0 8 11736.0 9 14240.0 10 17338.0 11 20918.0 12 24718.0 13 28139.0 14 30619.0 15 34468.0 16 38812.0 17 43160.0 18 39705.0 19 37102.0 20 39128.0 21 42514.0 22 47198.0 23 52625.0 24 59072.0 25 65994.0 26 75149.0 27 85072.0 28 96340.0 29 109953.0 30 125653.0 31 144189.0 32 165571.0 33 191737.0 34 227212.0 35 271500.0 36 310492.0 37 269363.0 38 83950.0 39 1396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49549162429805 19.14768111136736 13.674646897061265 22.68218036727332 2 15.01495711965205 21.127536172954358 40.91790514795525 22.939601559438337 3 18.9741170268476 26.924398107481533 30.059083366574153 24.042401499096712 4 12.142142819336039 16.836642948855687 38.77723636967817 32.2439778621301 5 12.895539338366738 38.656263363407575 34.52102042103935 13.927176877186342 6 29.912875562050758 37.33422350257063 18.55004047816807 14.202860457210544 7 26.916858130784448 33.940655762412746 22.054633431650377 17.087852675152426 8 25.371548740416213 34.731461117196055 20.919412924424975 18.97757721796276 9 27.175824645689218 16.471499877684888 20.786840155525947 35.565835321099954 10-11 24.231869519988113 26.712122550380357 28.4952381553232 20.56076977430833 12-13 26.19421185846474 24.422961968212768 28.25835097041789 21.124475202904602 14-15 23.24411573984823 25.401514222120962 27.1579593052805 24.196410732750312 16-17 21.594249225913956 28.616210346710634 27.75723245574373 22.03230797163168 18-19 21.3667431956396 27.641003172155138 30.007518533447836 20.984735098757426 20-21 22.724067610047737 26.742092749543634 29.512625397323394 21.021214243085236 22-23 22.093422754793316 26.707380776255867 29.22583008754001 21.97336638141081 24-25 21.999116679486328 26.72970304615832 29.726680855667215 21.544499418688137 26-27 22.13045092156368 26.709609907583253 29.73830246141324 21.421636709439827 28-29 21.649817504425624 26.790781658984365 30.10345857869473 21.455942257895284 30-31 21.847875449975366 27.23297370372903 29.630025126729542 21.28912571956606 32-33 21.589390829015944 27.29500786563269 29.4141348540457 21.701466451305667 34-35 22.055712474818403 26.833036151042243 29.40291782164504 21.708333552494317 36-37 22.334193647932615 26.712978710720385 29.47115199163999 21.481675649707014 38-39 22.301284565351818 26.763049245967203 29.621443399896595 21.314222788784384 40-41 22.07863745337722 26.78121151292289 29.846818693817085 21.293332339882802 42-43 21.696412495755208 27.16591319604723 29.28581083367271 21.85186347452485 44-45 21.78848423271341 27.506487751279845 28.86116738152886 21.843860634477885 46-47 21.994342055778176 27.169493739354095 28.378158266393267 22.45800593847446 48-49 22.11637828915122 27.279518646553807 28.450477437565553 22.15362562672942 50-51 22.43915032310634 27.514040209490343 28.286282423316834 21.760527044086487 52-53 22.085886449999254 27.654114409444052 28.47281308949699 21.787186051059702 54-55 21.812769268221842 27.383065418541296 28.61604289630003 22.188122416936825 56-57 22.023441627290154 28.037847741112863 27.819043556217633 22.119667075379347 58-59 21.95718430322543 27.454593397099053 28.238054095903852 22.350168203771663 60-61 22.06332473531419 27.3576545132062 28.40237256941456 22.176648182065055 62-63 22.474931327290218 27.21032861397093 28.417397542280458 21.897342516458398 64-65 22.143139851169725 27.28767741824659 28.57953922000651 21.989643510577174 66-67 21.735558690871557 27.62052890225294 28.372922931065435 22.270989475810072 68-69 21.729271494758677 27.747525190735047 28.393940865429872 22.129262449076403 70-71 21.913235720816395 27.400376412753154 28.039997580579417 22.646390285851037 72-73 21.737671063273684 27.49446010680647 27.77666321307492 22.991205616844923 74-75 21.86419577260048 27.688130363527176 27.804433662043763 22.64324020182858 76-77 22.049890609846816 27.173889211533176 28.187578898514037 22.58864128010597 78-79 21.79393657058376 27.27483841138984 28.25979621357076 22.671428804455637 80-81 21.716615455804735 27.91419097801257 27.80794532269158 22.561248243491118 82-83 21.914824349251322 27.547898849529517 27.785595158158994 22.751681643060166 84-85 21.91359415709506 27.286817110572812 27.609770605865734 23.189818126466385 86-87 21.945665013987448 27.213509881001485 28.079079578328297 22.761745526682773 88-89 22.21698548404241 26.8824105350933 28.048328291681678 22.852275689182612 90-91 21.535517175457606 27.106601996730223 28.53750245988133 22.820378367930836 92-93 21.136735553489892 27.370485831377632 28.587717740335712 22.905060874796764 94-95 21.544948747272485 27.274975618996837 27.92575546029229 23.25432017343839 96-97 21.622414837500813 26.977648982951447 27.661534774866247 23.73840140468149 98-99 21.78893987773205 26.930320745440746 27.58580636241101 23.694933014416193 100-101 22.08577319920483 26.97040646172349 27.75938148204754 23.184438857024137 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4182.0 1 2682.0 2 1205.0 3 1218.5 4 1080.0 5 1070.5 6 1547.5 7 2678.0 8 4079.5 9 4624.5 10 4029.0 11 3384.0 12 3367.5 13 3515.5 14 3713.5 15 3949.0 16 4211.5 17 4582.5 18 4952.5 19 5405.0 20 6065.0 21 6866.5 22 7947.5 23 9413.0 24 11053.0 25 13187.0 26 16507.5 27 20741.5 28 24673.5 29 29203.5 30 34597.5 31 40344.5 32 47066.0 33 53275.5 34 59257.0 35 65749.5 36 74078.0 37 81426.0 38 85172.0 39 87344.0 40 88655.0 41 89447.0 42 89956.0 43 92152.0 44 94794.0 45 96908.5 46 99443.5 47 105979.5 48 125385.0 49 126207.0 50 107338.5 51 98843.0 52 93957.0 53 90009.0 54 85409.0 55 79438.5 56 73121.0 57 66641.0 58 60419.5 59 54693.5 60 47014.0 61 38009.5 62 30045.0 63 23156.0 64 17325.5 65 12744.0 66 9001.0 67 6475.0 68 4619.5 69 3164.5 70 2304.0 71 1621.0 72 1118.0 73 776.0 74 592.5 75 458.5 76 326.0 77 227.5 78 147.5 79 94.5 80 70.5 81 54.0 82 38.5 83 28.5 84 22.0 85 12.0 86 10.0 87 9.5 88 7.0 89 5.5 90 4.5 91 3.5 92 3.0 93 6.0 94 5.5 95 4.0 96 2.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08842092140553197 2 0.09934659565445313 3 0.09598484973170816 4 0.09840110461368111 5 0.09521444962441244 6 0.07945626561154535 7 0.09258808562226793 8 0.08873608508578931 9 0.0830981570278524 10-11 0.08975161249995185 12-13 0.08868355780574642 14-15 0.09486426775745985 16-17 0.0916425945814959 18-19 0.09486426775745985 20-21 0.10423163269844193 22-23 0.11377408857290033 24-25 0.13725378275207228 26-27 0.14236643800958027 28-29 0.14297925627674732 30-31 0.1440648200643004 32-33 0.1405279832080791 34-35 0.13566045525743795 36-37 0.13968754672739286 38-39 0.1316858910675259 40-41 0.12769381778426625 42-43 0.12727359954392314 44-45 0.11769612548276948 46-47 0.1371487281919865 48-49 0.15577840351386493 50-51 0.1659862049355333 52-53 0.1742680060889623 54-55 0.1707836965127839 56-57 0.1408606559816841 58-59 0.13170340016087353 60-61 0.12765879959757098 62-63 0.12408694455465447 64-65 0.11023725171667906 66-67 0.12476979919521204 68-69 0.12149559873920521 70-71 0.1308454545868397 72-73 0.1583172220492713 74-75 0.17239453310076588 76-77 0.1779799338786599 78-79 0.17433804246235282 80-81 0.18172687985505273 82-83 0.18114907977458095 84-85 0.15668887636794168 86-87 0.1586674039162239 88-89 0.17761224291835964 90-91 0.2609730363464265 92-93 0.526445909683194 94-95 0.17785737022522646 96-97 0.19174208124989714 98-99 0.21066941115868526 100-101 0.21720030297735127 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2855659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.27359002100906 #Duplication Level Percentage of deduplicated Percentage of total 1 71.48342916736135 33.792783237536824 2 13.555068848072969 12.815935348607063 3 5.566500310687823 7.8944536061782715 4 2.886006376579062 5.457275289776659 5 1.6992210941789363 4.016414068063273 6 1.0805501811045013 3.0648891755196774 7 0.7451448701704431 2.4657971169086883 8 0.5391526812736467 2.0390146250606516 9 0.39104766794530793 1.6637604419806356 >10 1.8935446071556565 15.481401472265475 >50 0.10650890002398561 3.4596178352680083 >100 0.049905760790585695 4.230006083524832 >500 0.0021105186608735855 0.6471626146102057 >1k 0.0014321376626951496 1.35308368924244 >5k 3.015026658305578E-4 1.1475181610187117 >10k+ 7.537566645763944E-5 0.4708872344386497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13215 0.4627653371778633 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9491 0.3323576099247144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8906 0.31187197070798717 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7258 0.2541619990341984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6549 0.22933410466725893 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3728 0.13054779999992996 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3268 0.11443943412011028 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3087 0.10810114232826819 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3023 0.10585997837977154 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.5018186695260186E-5 0.0 3 0.0 0.0 0.0 3.5018186695260186E-5 0.0 4 0.0 0.0 0.0 3.5018186695260186E-5 3.5018186695260186E-5 5 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 6 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 7 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 8 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 9 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 10-11 0.0 3.5018186695260186E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 12-13 0.0 7.003637339052037E-5 0.0 3.5018186695260186E-5 3.5018186695260186E-5 14-15 0.0 7.003637339052037E-5 0.0 1.0505456008578054E-4 5.252728004289028E-5 16-17 0.0 7.003637339052037E-5 0.0 1.92600026823931E-4 7.003637339052037E-5 18-19 0.0 1.0505456008578054E-4 0.0 2.2761821351919117E-4 1.0505456008578054E-4 20-21 0.0 1.0505456008578054E-4 0.0 4.2021824034312223E-4 1.0505456008578054E-4 22-23 0.0 1.0505456008578054E-4 0.0 0.0010680546942054357 1.2256365343341064E-4 24-25 0.0 1.0505456008578054E-4 0.0 0.0015057820278961877 1.4007274678104074E-4 26-27 0.0 1.0505456008578054E-4 0.0 0.002153618481758501 1.4007274678104074E-4 28-29 0.0 1.4007274678104074E-4 0.0 0.004692437017164865 1.4007274678104074E-4 30-31 0.0 1.4007274678104074E-4 0.0 0.012781638143769968 1.4007274678104074E-4 32-33 0.0 1.4007274678104074E-4 0.0 0.03002809509118561 1.7509093347630093E-4 34-35 0.0 1.750909334763009E-4 0.0 0.057237226153402765 1.92600026823931E-4 36-37 0.0 2.101091201715611E-4 0.0 0.0990314319741958 2.101091201715611E-4 38-39 0.0 2.101091201715611E-4 0.0 0.15961289495699593 2.101091201715611E-4 40-41 0.0 2.101091201715611E-4 0.0 0.22364364932927916 2.101091201715611E-4 42-43 0.0 2.101091201715611E-4 0.0 0.2905283859172261 2.101091201715611E-4 44-45 0.0 2.2761821351919117E-4 0.0 0.37101768803628166 2.101091201715611E-4 46-47 0.0 2.451273068668213E-4 0.0 0.4687009198227099 2.101091201715611E-4 48-49 0.0 2.451273068668213E-4 0.0 0.5724598070007658 2.451273068668213E-4 50-51 0.0 3.1516368025734164E-4 0.0 0.6857611500532801 2.451273068668213E-4 52-53 0.0 3.85200053647862E-4 0.0 0.7986247657721037 2.451273068668213E-4 54-55 0.0 5.077637070812727E-4 0.0 0.9207682009651712 2.451273068668213E-4 56-57 0.0 5.60290987124163E-4 0.0 1.0588449111045821 2.451273068668213E-4 58-59 0.0 6.303273605146833E-4 0.0 1.2195433698491311 2.451273068668213E-4 60-61 0.0 6.653455472099435E-4 0.0 1.4118457420861525 2.451273068668213E-4 62-63 0.0 7.003637339052037E-4 0.0 1.6009789684272526 2.451273068668213E-4 64-65 0.0 7.003637339052037E-4 0.0 1.7849295031374544 2.451273068668213E-4 66-67 0.0 7.353819206004638E-4 0.0 1.9907138772521509 2.451273068668213E-4 68-69 0.0 7.353819206004638E-4 0.0 2.2260360918442994 2.451273068668213E-4 70-71 0.0 7.528910139480939E-4 0.0 2.4977772205995183 2.451273068668213E-4 72-73 0.0 7.70400107295724E-4 0.0 2.7854166061143855 3.1516368025734164E-4 74-75 0.0 7.70400107295724E-4 0.0 3.07610257387174 3.1516368025734164E-4 76-77 0.0 7.70400107295724E-4 0.0 3.3738622153415374 3.1516368025734164E-4 78-79 0.0 7.70400107295724E-4 0.0 3.7157272629540152 3.1516368025734164E-4 80-81 0.0 8.054182939909842E-4 0.0 4.097057806972051 3.501818669526018E-4 82-83 0.0 8.054182939909842E-4 0.0 4.480629514938583 3.85200053647862E-4 84-85 0.0 8.054182939909842E-4 0.0 4.875512097207684 3.85200053647862E-4 86-87 0.0 8.754546673815046E-4 0.0 5.297673846912394 3.85200053647862E-4 88-89 0.0 8.754546673815046E-4 0.0 5.733107489374607 3.85200053647862E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13805 0.0 28.875923 1 TACATGG 13875 0.0 28.286102 2 ACATGGG 14145 0.0 26.806768 3 CATGGGA 9045 0.0 24.817223 4 ACCGTAT 225 2.8503564E-9 23.199184 8 CATGGGG 5430 0.0 22.111658 4 GAGTACT 10075 0.0 21.807127 12-13 AGTACTT 11000 0.0 20.943974 12-13 GTATCAA 25355 0.0 20.756824 1 GTACCGT 315 3.255991E-10 19.582355 6 ATGGGAT 3960 0.0 19.534424 5 TACCGTA 270 2.3295797E-8 19.331976 7 GTACTTT 11380 0.0 19.285887 14-15 ATGGGAG 3955 0.0 18.719158 5 TATCAAC 28695 0.0 18.341122 2 ATCAACG 29020 0.0 18.282896 3 TCAACGC 29120 0.0 18.138626 4 CAACGCA 29190 0.0 18.095446 5 AACGCAG 29825 0.0 17.738894 6 TACTTTT 13085 0.0 17.461948 14-15 >>END_MODULE