FastQCFastQC Report
Fri 27 May 2016
SRR939667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855659
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136560.4782083575104731No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102490.3589013954397216No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA96400.3375753197423082No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA79790.279410111641481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76940.26942992843333186No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT51570.18058878878745677No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA39560.1385319465664493No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG38770.13576550981752375No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT35040.12270372618019168No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC31680.11093761545058425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG138000.026.2389971
TACATGG138750.025.7157422
ACATGGG138000.024.4211623
CATGGGA90700.021.692094
GAGTACT111300.021.0483212-13
GTATCAA248050.020.6533091
CATGGGG49300.020.62244
AGTACTT117000.020.28397212-13
GTACTTT118700.019.5381714-15
GGTATCA196150.018.6283051
ATGGGAT40650.018.4106435
ATCAACG281100.018.0840343
TCAACGC281900.018.0327134
CAACGCA285550.017.8186955
AACGCAG285650.017.800536
TATCAAC288050.017.6979682
TACTTTT133700.017.4867514-15
ACTTTTT137200.017.33180816-17
ATGGGAG36600.016.4611915
ACGCAGA305600.016.4539557
Produced by FastQC (version 0.11.5)