##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939667_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2855659 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.547488688250244 31.0 31.0 34.0 30.0 34.0 2 31.746748473819878 31.0 31.0 34.0 30.0 34.0 3 31.79067773848348 31.0 31.0 34.0 30.0 34.0 4 35.57856032530495 37.0 35.0 37.0 33.0 37.0 5 35.31709143143492 37.0 35.0 37.0 33.0 37.0 6 35.34152642174713 37.0 35.0 37.0 32.0 37.0 7 35.2693963109741 37.0 35.0 37.0 32.0 37.0 8 35.23825743900095 37.0 35.0 37.0 32.0 37.0 9 36.84648307098291 39.0 37.0 39.0 32.0 39.0 10-11 36.729170569735395 39.0 35.0 39.0 32.0 39.0 12-13 36.66864846257904 39.0 35.0 39.0 32.0 39.0 14-15 37.69374004389179 39.5 36.5 41.0 32.0 41.0 16-17 37.66880867078317 39.0 36.5 41.0 32.0 41.0 18-19 37.68621200920698 39.5 36.5 41.0 32.0 41.0 20-21 37.62590648953534 39.0 36.5 41.0 32.0 41.0 22-23 37.48686677926181 39.0 36.0 41.0 32.0 41.0 24-25 37.3624338550226 39.0 36.0 41.0 31.5 41.0 26-27 37.08015120152651 39.0 36.0 40.5 31.0 41.0 28-29 36.8723447022211 39.0 36.0 40.0 30.5 41.0 30-31 36.63736233913083 38.5 35.0 40.0 30.0 41.0 32-33 36.45146514342224 38.0 35.0 40.0 30.0 41.0 34-35 36.42516490939569 38.0 35.0 40.0 30.0 41.0 36-37 36.30117128830858 38.0 35.0 40.0 30.0 41.0 38-39 36.3133998141935 38.0 35.0 40.0 30.0 41.0 40-41 36.20265024640547 38.0 35.0 40.0 30.0 41.0 42-43 36.01560077726367 38.0 35.0 40.0 29.5 41.0 44-45 35.79624510489523 38.0 35.0 40.0 28.5 41.0 46-47 35.46890034839594 38.0 34.5 40.0 27.0 41.0 48-49 35.1483934181217 38.0 34.0 40.0 26.5 41.0 50-51 34.89119201557329 38.0 34.0 40.0 26.0 41.0 52-53 34.62812033929821 38.0 33.5 40.0 25.0 41.0 54-55 34.34524780444724 38.0 33.0 40.0 24.0 41.0 56-57 32.38685448787828 37.0 30.5 39.5 15.5 41.0 58-59 27.42465592005208 32.5 17.5 38.5 6.5 40.0 60-61 28.134151871774606 33.0 19.5 38.0 7.5 40.0 62-63 31.172096703422923 34.5 29.0 38.5 8.5 40.0 64-65 31.372250503298886 35.0 30.5 39.0 4.5 40.0 66-67 24.753152774893643 27.0 18.0 35.5 2.0 39.0 68-69 17.35263961838581 13.0 7.0 28.0 2.0 35.0 70-71 16.239379596793597 12.5 7.0 26.5 2.0 33.5 72-73 17.371821530511873 13.5 7.0 28.5 2.0 34.0 74-75 18.361513051803456 17.5 8.5 29.5 2.0 34.5 76-77 19.527018632126595 21.5 8.0 30.0 2.0 34.5 78-79 22.5785872893087 27.5 10.5 33.5 2.0 35.5 80-81 26.20886107199774 31.5 22.5 34.5 2.0 36.0 82-83 27.502746826564376 33.0 26.5 35.0 2.0 36.0 84-85 27.553271066328296 34.0 27.0 35.0 2.0 36.0 86-87 27.303221953321458 34.0 26.5 35.0 2.0 36.0 88-89 26.870851526740413 33.0 26.0 35.0 2.0 35.0 90-91 25.54772628664697 32.5 17.5 35.0 2.0 35.0 92-93 23.137373019677767 31.5 2.0 34.5 2.0 35.0 94-95 15.878795402392232 4.5 2.0 32.0 2.0 35.0 96-97 13.328156127885016 2.0 2.0 30.0 2.0 34.0 98-99 13.021854325043712 2.0 2.0 29.5 2.0 34.0 100-101 12.721555164674774 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38162746139580506 1101 2 0.35212746995949473 1101 3 0.39957644757463484 1101 4 0.21099386526378083 1101 5 0.3407152175999144 1101 6 0.3577384219224484 1101 7 0.38398993097567313 1101 8 0.35841440156834636 1101 9 0.34848303798669633 1101 10-11 0.4399629093316122 1101 12-13 0.3618991972335408 1101 14-15 0.45914635982092733 1101 16-17 0.5628984690874645 1101 18-19 0.5317315056728589 1101 20-21 0.5295927948998411 1101 22-23 0.5460298804500852 1101 24-25 0.42704993807424785 1101 26-27 0.5432287251912342 1101 28-29 0.5201302741734253 1101 30-31 0.5414143105642566 1101 32-33 0.36265721426769915 1101 34-35 0.3442941786420519 1101 36-37 0.1859172622277967 1101 38-39 0.3053583309730783 1101 40-41 0.3438272156513662 1101 42-43 0.4074684319866293 1101 44-45 0.21274445059426483 1101 46-47 0.33363326312333186 1101 48-49 0.2488701133590112 1101 50-51 0.05554875050909658 1101 52-53 0.04604680733807953 1101 54-55 0.016550406346468094 1101 56-57 -2.2233641840875293 1101 58-59 -9.627386837027181 1101 60-61 -10.080492960697661 1101 62-63 -3.984314202802903 1101 64-65 -5.113933201107994 1101 66-67 -9.73233195977883 1101 68-69 -4.898104217627061 1101 70-71 -4.618596795000876 1101 72-73 -4.700916653010314 1101 74-75 -6.817280714679438 1101 76-77 -8.31856625650913 1101 78-79 -11.311016028949176 1101 80-81 -8.402752673416582 1101 82-83 -4.627983842653077 1101 84-85 -3.9911115092322333 1101 86-87 -3.646274838026489 1101 88-89 -3.7971545937820004 1101 90-91 -10.099049879371432 1101 92-93 -17.0222873444929 1101 94-95 -11.31140936773269 1101 96-97 -9.374194047904094 1101 98-99 -9.288498736764911 1101 100-101 -9.199842232424363 1102 1 0.31852048783685305 1102 2 0.3130948036325272 1102 3 0.3064507506826821 1102 4 0.23796930498718183 1102 5 0.278460682120091 1102 6 0.29051812611690764 1102 7 0.3822547500735354 1102 8 0.3722603891109131 1102 9 0.349750682298982 1102 10-11 0.38824192792029066 1102 12-13 0.22026771166687098 1102 14-15 0.32605315773734134 1102 16-17 0.3177964342645083 1102 18-19 0.31341011667836227 1102 20-21 0.38536916026133383 1102 22-23 0.36480384111452224 1102 24-25 0.35524386281662856 1102 26-27 0.36642233595147644 1102 28-29 0.3924379554262529 1102 30-31 0.28606700726469114 1102 32-33 0.43570594023439924 1102 34-35 0.11446284484905078 1102 36-37 0.18990350762310726 1102 38-39 0.09605439969799079 1102 40-41 0.1820056631049738 1102 42-43 0.13331000565886342 1102 44-45 0.22575643284005764 1102 46-47 0.17430747806644575 1102 48-49 0.06151907913933741 1102 50-51 0.10813810342158803 1102 52-53 0.04779534419692055 1102 54-55 -0.11383848787091466 1102 56-57 -2.9031791002074385 1102 58-59 -10.463048741058618 1102 60-61 -10.660702275876055 1102 62-63 -4.4052515472763005 1102 64-65 -5.492762182065679 1102 66-67 -6.983040684639562 1102 68-69 -4.563831567408478 1102 70-71 -4.313978430138633 1102 72-73 -4.636542347559114 1102 74-75 -5.378877684080081 1102 76-77 -7.610871522755048 1102 78-79 -10.219977063609367 1102 80-81 -6.0751109042318845 1102 82-83 -3.5927585612485373 1102 84-85 -3.2352057551111777 1102 86-87 -3.095271797867081 1102 88-89 -3.1434598675260013 1102 90-91 -9.182071287329833 1102 92-93 -13.826973504503822 1102 94-95 -10.755049104937449 1102 96-97 -9.324932827572672 1102 98-99 -9.456230417312922 1102 100-101 -9.309200571649416 1103 1 0.20939984499820596 1103 2 0.08241308756002397 1103 3 0.08293174290772853 1103 4 0.1976432811981539 1103 5 0.15921393754614854 1103 6 0.21391771554322503 1103 7 0.1980398512787218 1103 8 0.20764636931171054 1103 9 0.3276826127768686 1103 10-11 0.34874188990420407 1103 12-13 0.33521910425177026 1103 14-15 0.4230486643959992 1103 16-17 0.35036963648931874 1103 18-19 0.37181790481766086 1103 20-21 0.23028920154202126 1103 22-23 0.4599858743421734 1103 24-25 0.3906612262232585 1103 26-27 0.43272364503392424 1103 28-29 0.34910154979094443 1103 30-31 0.3964338730859964 1103 32-33 0.48503014456844085 1103 34-35 0.3668581902982524 1103 36-37 0.3565519859430495 1103 38-39 0.3079555068432569 1103 40-41 0.4404107396076782 1103 42-43 0.3412858859172516 1103 44-45 0.1686688395344973 1103 46-47 0.21681072006725088 1103 48-49 0.2531670523504914 1103 50-51 0.205436409499427 1103 52-53 0.19337959022637108 1103 54-55 0.03733857508637328 1103 56-57 -3.1234784383022642 1103 58-59 -10.873161045348905 1103 60-61 -11.16465395451203 1103 62-63 -5.064890179165253 1103 64-65 -5.762598202489087 1103 66-67 -6.919898099875663 1103 68-69 -4.292118934956992 1103 70-71 -4.317788944163908 1103 72-73 -4.503847414119161 1103 74-75 -5.327478546061501 1103 76-77 -7.001561331201849 1103 78-79 -9.2726147150556 1103 80-81 -5.4403855664337435 1103 82-83 -3.3919472502147805 1103 84-85 -2.92246466536292 1103 86-87 -2.7880304786930985 1103 88-89 -2.7661170268048743 1103 90-91 -5.457748624906788 1103 92-93 -10.184819345836924 1103 94-95 -8.640031782364881 1103 96-97 -8.240966668265617 1103 98-99 -8.5306318767051 1103 100-101 -8.391968825615983 1104 1 0.22193550833629416 1104 2 0.25753691859505423 1104 3 0.16894725645979847 1104 4 0.10778504296705194 1104 5 0.18815572705320704 1104 6 0.157784116716158 1104 7 0.21463221932437193 1104 8 0.2189006290993163 1104 9 0.23281961943402507 1104 10-11 0.3156829661444931 1104 12-13 0.3810739841851998 1104 14-15 0.3673744787088893 1104 16-17 0.34816990198305575 1104 18-19 0.33676084347671775 1104 20-21 0.3101257941081883 1104 22-23 0.3525173769574863 1104 24-25 0.33910987101640444 1104 26-27 0.47449036717689097 1104 28-29 0.44058989976716845 1104 30-31 0.38379666829919046 1104 32-33 0.6252120771251128 1104 34-35 0.4455884763974822 1104 36-37 0.43400681238146177 1104 38-39 0.477712253158046 1104 40-41 0.34739926790059883 1104 42-43 0.4496563685610866 1104 44-45 0.5237563137776462 1104 46-47 0.5634496981144821 1104 48-49 0.48069246247887776 1104 50-51 0.5404352702539086 1104 52-53 0.44090396017406164 1104 54-55 0.5112243434331774 1104 56-57 -2.384426835433345 1104 58-59 -10.207294079506458 1104 60-61 -10.259323028454016 1104 62-63 -4.015474413988521 1104 64-65 -5.292109862419345 1104 66-67 -6.465465196483681 1104 68-69 -4.129010924574999 1104 70-71 -4.0804481908749555 1104 72-73 -4.302571706796288 1104 74-75 -4.765094204248218 1104 76-77 -6.418188619599377 1104 78-79 -9.090315123202693 1104 80-81 -4.6179766070107 1104 82-83 -2.2860486826921935 1104 84-85 -1.7463888213305871 1104 86-87 -1.7047905139601767 1104 88-89 -1.638035862991277 1104 90-91 -4.14790678190473 1104 92-93 -6.492698219271702 1104 94-95 -7.691063542548745 1104 96-97 -7.8867759783781555 1104 98-99 -8.052032108516798 1104 100-101 -8.138901530878305 1105 1 0.29573925313518146 1105 2 0.3379451093794579 1105 3 0.33783161845702736 1105 4 0.21846898887551447 1105 5 0.2536559967403704 1105 6 0.24721767735724143 1105 7 0.3429175868694472 1105 8 0.224476892776309 1105 9 0.3301078186943158 1105 10-11 0.387372556220015 1105 12-13 0.27088486606222517 1105 14-15 0.5140985020560933 1105 16-17 0.47466329464756996 1105 18-19 0.5003530764864266 1105 20-21 0.4549331122619904 1105 22-23 0.4352179086264627 1105 24-25 0.4925599403645293 1105 26-27 0.4613710498706851 1105 28-29 0.528881624223942 1105 30-31 0.5673057798726262 1105 32-33 0.5903932972668855 1105 34-35 0.5696989903441576 1105 36-37 0.513578009792468 1105 38-39 0.544532785982824 1105 40-41 0.49345364839066264 1105 42-43 0.5370509016170288 1105 44-45 0.6283472561416446 1105 46-47 0.6504081566597151 1105 48-49 0.5760092967368564 1105 50-51 0.6847311459666514 1105 52-53 0.757689096558714 1105 54-55 0.6015461861670346 1105 56-57 1.7402136619468322 1105 58-59 -8.500990864450205 1105 60-61 -9.7753908451438 1105 62-63 -3.459675417610036 1105 64-65 -4.828522204459723 1105 66-67 -6.24420397557137 1105 68-69 -3.9693395094879307 1105 70-71 -3.9432027081349332 1105 72-73 -2.0597095073048415 1105 74-75 -4.092993582041409 1105 76-77 -6.265097571286848 1105 78-79 -9.034943051234746 1105 80-81 -4.390979477252646 1105 82-83 -1.8014432446431883 1105 84-85 -1.261431586009273 1105 86-87 -1.1040823443654766 1105 88-89 -1.1743042113500373 1105 90-91 -3.703078755629491 1105 92-93 -6.425016132278326 1105 94-95 -7.719415850300324 1105 96-97 -7.962499558667877 1105 98-99 -8.386764684799738 1105 100-101 -8.255349799101452 1106 1 0.11097871821283434 1106 2 0.09639273870087095 1106 3 0.1112036497578579 1106 4 0.10967547506582065 1106 5 0.004943645924456064 1106 6 0.11698274987312374 1106 7 0.18915823343371585 1106 8 0.11187351269720125 1106 9 0.11103280285554717 1106 10-11 0.19461815132968496 1106 12-13 0.23017837131042285 1106 14-15 0.2456510439646209 1106 16-17 0.3226573358454985 1106 18-19 0.21303950308871578 1106 20-21 0.28264839110995865 1106 22-23 0.24538554274231927 1106 24-25 0.3455252149317545 1106 26-27 0.2692985682350937 1106 28-29 0.27917014668074813 1106 30-31 0.39346928734681086 1106 32-33 0.3365684483773137 1106 34-35 0.39112970214704035 1106 36-37 0.39200945788410735 1106 38-39 0.3669566314649302 1106 40-41 0.29683158712458635 1106 42-43 0.22443039854345415 1106 44-45 0.2813566224196222 1106 46-47 0.3526499847106095 1106 48-49 0.20796594131486046 1106 50-51 0.32291157095937706 1106 52-53 0.2976610148477832 1106 54-55 0.2579643125689799 1106 56-57 1.4521830631557222 1106 58-59 -8.540453801728678 1106 60-61 -9.80546224803074 1106 62-63 -3.6562675136357896 1106 64-65 -5.133327344782664 1106 66-67 -6.7283206859016715 1106 68-69 -4.146264562141138 1106 70-71 -4.028968362832629 1106 72-73 -4.521368554238968 1106 74-75 -4.895404499662682 1106 76-77 -6.3687755904988474 1106 78-79 -8.99050802443726 1106 80-81 -4.685497550573309 1106 82-83 -2.2357923996234135 1106 84-85 -1.6418253292670926 1106 86-87 -1.5193766030254316 1106 88-89 -1.5455397210159667 1106 90-91 -4.245462447689562 1106 92-93 -6.447352650488632 1106 94-95 -7.346393128086664 1106 96-97 -7.249306291429301 1106 98-99 -6.763134952432141 1106 100-101 -7.343032373029981 1107 1 -0.029886439583897584 1107 2 -0.15300703548754058 1107 3 -0.11201211959701496 1107 4 -0.06660094790206728 1107 5 -0.14304871250640616 1107 6 -0.07147425795036355 1107 7 0.06651546247621098 1107 8 0.11916354102206128 1107 9 -0.02254089516409863 1107 10-11 -0.05640327929945954 1107 12-13 -0.0546697665193463 1107 14-15 0.032508940719942814 1107 16-17 0.048299655299111066 1107 18-19 0.033801763327922174 1107 20-21 0.034134208698802126 1107 22-23 0.02356418678726868 1107 24-25 0.13931700662682545 1107 26-27 -0.005039608774715987 1107 28-29 -0.012676361082796461 1107 30-31 -3.818214983652979E-5 1107 32-33 -0.06333516717278798 1107 34-35 -0.11921760991372565 1107 36-37 -0.10169519415669726 1107 38-39 -0.3923670431445103 1107 40-41 -0.35918644678771017 1107 42-43 -0.25616632617456503 1107 44-45 -0.1866510764075997 1107 46-47 -0.2646115994656455 1107 48-49 -0.4845325332938657 1107 50-51 -0.2925460851926829 1107 52-53 -0.25626870919804645 1107 54-55 -0.3533689122125594 1107 56-57 0.93461830932673 1107 58-59 -9.399436253913702 1107 60-61 -10.967612503992289 1107 62-63 -4.743731809340417 1107 64-65 -6.256179525272088 1107 66-67 -7.72649522645024 1107 68-69 -4.612151624139123 1107 70-71 -4.467298895887543 1107 72-73 -2.816063174781858 1107 74-75 -5.042694730776606 1107 76-77 -7.044319587077677 1107 78-79 -7.982573723322933 1107 80-81 -3.2009324227774307 1107 82-83 -2.056647227106577 1107 84-85 -1.7813919213193117 1107 86-87 -1.7430419676670112 1107 88-89 -1.5000615294888497 1107 90-91 -0.907750361640197 1107 92-93 -3.45262164720166 1107 94-95 -6.112702050984472 1107 96-97 -6.869299146096939 1107 98-99 -6.436365364350412 1107 100-101 -7.104232442932565 1108 1 -0.09971977323651515 1108 2 -0.10071334057781556 1108 3 -0.06334510441990915 1108 4 -0.034274895386829485 1108 5 -0.03624979518012594 1108 6 -0.20433742147481837 1108 7 -0.15566309890288466 1108 8 -0.12332568897983975 1108 9 -0.09964629470569975 1108 10-11 -0.11483301926777045 1108 12-13 -0.023669052858004136 1108 14-15 -0.0974787299437807 1108 16-17 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94-95 11.860381407107354 2316 96-97 13.836959773766777 2316 98-99 13.509657226199218 2316 100-101 12.218983870277475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 45.0 5 133.0 6 313.0 7 715.0 8 1595.0 9 2658.0 10 4627.0 11 7284.0 12 10685.0 13 15081.0 14 18167.0 15 24949.0 16 33625.0 17 45003.0 18 48769.0 19 47589.0 20 48679.0 21 49097.0 22 48720.0 23 41827.0 24 42538.0 25 50321.0 26 70999.0 27 105214.0 28 147639.0 29 199410.0 30 248529.0 31 273004.0 32 274557.0 33 251227.0 34 225150.0 35 203289.0 36 164530.0 37 106109.0 38 40720.0 39 2837.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.713298010025355 19.81869454609012 14.509795405261228 22.958212038623294 2 15.187463206543796 21.830964952492884 39.936339660027585 23.04523218093573 3 18.71293976338178 26.970835062358724 30.645963885693472 23.67026128856602 4 12.502963414049088 17.463849850419816 38.35699570571977 31.676191029811335 5 12.720462295739656 39.00259090979229 34.58338535259238 13.693561441875667 6 30.035834110445258 37.697743322994796 18.17426380390656 14.092158762653384 7 26.716004957174512 34.01610626478862 21.956543130674916 17.311345647361957 8 25.32284639210818 35.22939892107305 20.720685386510436 18.72706930030834 9 26.625798108247515 16.476967312973994 20.667523678422388 36.229710900356096 10-11 24.14605525379606 26.886841180967338 28.433769578230457 20.533333987006152 12-13 25.87140131227153 24.51020937724007 28.42424463144934 21.19414467903906 14-15 22.801628705273856 25.574962552210227 27.177911800934467 24.44549694158145 16-17 21.39257523394775 28.809304612350424 27.802461708488302 21.99565844521352 18-19 21.226167241170106 27.698658923899934 30.039278211002067 21.035895623927896 20-21 22.42337057750943 26.86672323271091 29.54643744228565 21.16346874749401 22-23 21.815737540619043 26.812411677273417 29.2919701805141 22.079880601593437 24-25 21.783333298315135 26.904447241231267 29.717984436511657 21.594235023941945 26-27 21.942239460550514 26.880183579791932 29.743049923450055 21.43452703620749 28-29 21.54678052160283 26.894621674496122 30.027558483640792 21.531039320260255 30-31 21.67690178575963 27.327514062964326 29.6359013036709 21.35968284760515 32-33 21.480252306541523 27.385597431549662 29.381728507123263 21.75242175478555 34-35 21.883131698295642 26.959613984161614 29.410857282035686 21.746397035507062 36-37 22.18225129623592 26.811890663946237 29.43151422090875 21.574343818909096 38-39 22.17666760853119 26.82315640536821 29.642194315298383 21.35798167080222 40-41 21.962115477460618 26.86576505158756 29.85504573581741 21.31707373513441 42-43 21.60958576300734 27.220436575208893 29.2742481494974 21.89572951228637 44-45 21.713225877986847 27.503508266009398 28.869790520801104 21.913475335202655 46-47 21.800300696945897 27.268746536875344 28.43277712403372 22.49817564214504 48-49 21.9892356633859 27.251872294804834 28.523388522294702 22.23550351951457 50-51 22.340493596255126 27.55314981160816 28.269731185575818 21.83662540656089 52-53 22.027983486591925 27.598813981613873 28.52050357587803 21.852698955916168 54-55 21.785842201582792 27.36246590954491 28.580820750266874 22.27087113860543 56-57 21.074258682431495 27.91873382843112 28.91748499338262 22.08952249575477 58-59 20.56160045298792 27.120286503478148 30.152097381021314 22.166015662512617 60-61 21.716180387691512 27.322721637001845 28.810482753280258 22.15061522202638 62-63 22.364013846162198 27.260809369079997 28.425115491203794 21.950061293554015 64-65 21.687526339003043 27.260999623453053 28.948501420290203 22.1029726172537 66-67 21.268595463074583 27.283112111291207 29.341594097700273 22.10669832793393 68-69 21.515529852628546 27.046643606096637 29.469678953626634 21.968147587648183 70-71 21.411344716763697 26.90149956996223 29.251568674817648 22.43558703845643 72-73 20.444404266729546 27.101518142514745 29.54928751252658 22.904790078229126 74-75 21.180016125016387 27.178654393459688 28.97373236973798 22.667597111785945 76-77 19.50298661854271 27.44564126984465 30.000983643465112 23.050388468147528 78-79 20.16610321055115 27.37460082388889 29.434358393434334 23.024937572125623 80-81 21.049285631450367 27.952728593906734 28.2137825708719 22.784203203771007 82-83 21.607188412171443 27.576316647882276 27.823265549034957 22.99322939091132 84-85 21.531341249910046 27.2752629126748 27.665523845942797 23.527871991472356 86-87 21.506255755037248 27.301485248282308 28.06493651796675 23.127322478713694 88-89 21.84446300410939 26.88481276624802 28.063666654410284 23.2070575752323 90-91 20.762653555408427 27.23521982022336 28.689015763980503 23.313110860387706 92-93 19.68457879246722 27.555081390658465 29.210366049246556 23.54997376762776 94-95 18.98425982231914 27.57285870808565 29.303894280869347 24.138987188725864 96-97 19.083696167275384 27.48391327572235 28.68667326506064 24.745717291941624 98-99 19.28110023402397 27.296558848036046 28.76950192362698 24.652838994313008 100-101 19.89399065981121 27.307469500180083 28.468224590081075 24.33031524992763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1343.0 1 999.0 2 672.5 3 721.0 4 777.5 5 902.0 6 1175.0 7 1711.5 8 2282.0 9 2626.5 10 2823.5 11 3089.5 12 3465.0 13 3783.5 14 4071.5 15 4411.5 16 4747.5 17 5182.0 18 5537.5 19 5933.0 20 6627.5 21 7520.0 22 8624.0 23 9974.5 24 11815.0 25 14275.5 26 17418.5 27 21150.0 28 25440.5 29 30353.5 30 35938.0 31 42163.0 32 49157.5 33 56382.5 34 63403.0 35 70813.5 36 78302.5 37 84055.0 38 88088.0 39 91495.5 40 93507.5 41 95482.0 42 98029.5 43 100547.0 44 103173.5 45 106404.0 46 110624.5 47 114978.0 48 120212.0 49 117996.5 50 109152.0 51 101889.5 52 95519.5 53 89636.5 54 83063.5 55 76308.0 56 68783.5 57 60692.5 58 53067.5 59 45231.5 60 36768.0 61 28888.5 62 22275.5 63 16531.0 64 11908.0 65 8465.0 66 5876.0 67 4053.5 68 2721.0 69 1777.0 70 1158.5 71 755.0 72 516.5 73 378.5 74 262.5 75 180.0 76 116.5 77 62.0 78 32.0 79 18.5 80 16.5 81 9.0 82 6.0 83 2.0 84 0.5 85 1.0 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024442694313291605 2 0.006723491845489955 3 1.4007274678104072E-4 4 0.0 5 0.14536049297202502 6 0.0 7 0.0 8 0.001540800214591448 9 0.0 10-11 0.0 12-13 0.0 14-15 5.77800080471793E-4 16-17 0.0 18-19 1.5758184012867082E-4 20-21 0.0 22-23 3.3267277360497175E-4 24-25 3.501818669526018E-5 26-27 2.451273068668213E-4 28-29 0.0033442368293973472 30-31 0.013149329104070198 32-33 0.0029765458690971156 34-35 0.0044998369903409335 36-37 0.11097263363727952 38-39 0.11069248814371743 40-41 0.12319398079392532 42-43 0.12592539935615563 44-45 0.12139054417911943 46-47 0.11505225238727733 48-49 0.11475459780036762 50-51 0.112986179372257 52-53 0.09046948532720468 54-55 0.07777539265017287 56-57 0.5397002933473499 58-59 1.5444771241944504 60-61 0.5116682348977942 62-63 0.004850018857293536 64-65 0.11993728943126612 66-67 1.873367933636334 68-69 2.814026464644413 70-71 2.7312784894835134 72-73 2.576463086103768 74-75 1.9708935835826336 76-77 1.3866501567589127 78-79 0.9242700196346973 80-81 0.05112655257507987 82-83 2.626364002144514E-4 84-85 1.92600026823931E-4 86-87 2.976545869097116E-4 88-89 1.4007274678104072E-4 90-91 0.24558254329385965 92-93 0.38140058039142627 94-95 1.9601955275472316 96-97 2.385158732187562 98-99 1.3830257744359533 100-101 0.9237797650209637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2855659.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.935075067322465 #Duplication Level Percentage of deduplicated Percentage of total 1 65.54780821401813 26.832044497392882 2 15.624908487894585 12.792136037440175 3 6.816706477297561 8.371271740802369 4 3.624103549126777 5.934118033410175 5 2.173824154607327 4.449282747600487 6 1.3829907114319469 3.396769715392587 7 0.9623660896707656 2.757616968604286 8 0.6812428392508343 2.2309381411047036 9 0.4985032392048484 1.836564076633849 >10 2.4565643023982284 17.54270860332608 >50 0.15439914872545388 4.33798399129903 >100 0.070844520940653 5.2007152015028595 >500 0.003425830109240407 0.9003891717705458 >1k 0.0017985608073157868 1.511119620007554 >5k 3.425830109172927E-4 1.0682523971423639 >10k+ 1.7129150545864636E-4 0.8380890565700102 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13656 0.4782083575104731 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10249 0.3589013954397216 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9640 0.3375753197423082 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7979 0.279410111641481 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7694 0.26942992843333186 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5157 0.18058878878745677 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3956 0.1385319465664493 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3877 0.13576550981752375 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3504 0.12270372618019168 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3168 0.11093761545058425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.5018186695260186E-5 0.0 3 0.0 0.0 0.0 3.5018186695260186E-5 0.0 4 0.0 0.0 0.0 3.5018186695260186E-5 0.0 5 0.0 0.0 0.0 3.5018186695260186E-5 0.0 6 0.0 0.0 0.0 3.5018186695260186E-5 0.0 7 0.0 0.0 0.0 3.5018186695260186E-5 0.0 8 0.0 0.0 0.0 3.5018186695260186E-5 0.0 9 0.0 0.0 0.0 3.5018186695260186E-5 0.0 10-11 0.0 0.0 0.0 3.5018186695260186E-5 0.0 12-13 0.0 0.0 0.0 3.5018186695260186E-5 0.0 14-15 0.0 0.0 0.0 1.0505456008578054E-4 3.5018186695260186E-5 16-17 0.0 0.0 0.0 2.2761821351919117E-4 3.5018186695260186E-5 18-19 0.0 3.5018186695260186E-5 0.0 2.626364002144514E-4 3.5018186695260186E-5 20-21 0.0 3.5018186695260186E-5 0.0 4.552364270383824E-4 3.5018186695260186E-5 22-23 0.0 3.5018186695260186E-5 0.0 0.0010680546942054357 3.5018186695260186E-5 24-25 0.0 3.5018186695260186E-5 0.0 0.0015933274946343383 3.5018186695260186E-5 26-27 0.0 3.5018186695260186E-5 0.0 0.0022586730418442816 3.5018186695260186E-5 28-29 0.0 3.5018186695260186E-5 0.0 0.004885037043988795 3.5018186695260186E-5 30-31 0.0 3.5018186695260186E-5 0.0 0.013236874570808348 3.5018186695260186E-5 32-33 0.0 3.5018186695260186E-5 0.0 0.03142882255899602 7.003637339052037E-5 34-35 0.0 5.252728004289028E-5 0.0 0.060021171995675957 7.003637339052037E-5 36-37 0.0 7.003637339052037E-5 0.0 0.10333866893771279 7.003637339052037E-5 38-39 0.0 7.003637339052037E-5 0.0 0.16631887770913822 7.003637339052037E-5 40-41 0.0 7.003637339052037E-5 0.0 0.2330285233636089 7.003637339052037E-5 42-43 0.0 7.003637339052037E-5 0.0 0.30276724216721956 7.003637339052037E-5 44-45 0.0 7.003637339052037E-5 0.0 0.3853751445813383 7.003637339052037E-5 46-47 0.0 7.003637339052037E-5 0.0 0.48179772164673723 7.003637339052037E-5 48-49 0.0 7.003637339052037E-5 0.0 0.5694832611316687 7.003637339052037E-5 50-51 0.0 8.754546673815045E-5 0.0 0.6568361278429953 7.003637339052037E-5 52-53 0.0 1.2256365343341064E-4 0.0 0.7416151578322201 8.754546673815045E-5 54-55 1.7509093347630093E-5 2.2761821351919117E-4 0.0 0.8329075705467635 1.0505456008578054E-4 56-57 3.5018186695260186E-5 2.801454935620815E-4 0.0 0.9079165264480108 1.0505456008578054E-4 58-59 7.003637339052037E-5 3.1516368025734164E-4 0.0 0.9629651159329597 1.0505456008578054E-4 60-61 7.003637339052037E-5 3.1516368025734164E-4 0.0 1.0101171043181276 1.5758184012867085E-4 62-63 1.2256365343341064E-4 3.1516368025734164E-4 0.0 1.0532770194200358 1.7509093347630093E-4 64-65 1.4007274678104074E-4 3.1516368025734164E-4 0.0 1.0927250067322465 1.7509093347630093E-4 66-67 1.4007274678104074E-4 3.1516368025734164E-4 0.0 1.1262724295863058 1.7509093347630093E-4 68-69 1.5758184012867085E-4 3.1516368025734164E-4 0.0 1.173546981624907 1.7509093347630093E-4 70-71 1.7509093347630093E-4 3.3267277360497175E-4 0.0 1.2505169559810887 1.7509093347630093E-4 72-73 1.7509093347630093E-4 3.5018186695260186E-4 0.0 1.3619098078587113 1.7509093347630093E-4 74-75 1.7509093347630093E-4 3.85200053647862E-4 0.0 1.4996538452245174 1.7509093347630093E-4 76-77 1.7509093347630093E-4 4.202182403431222E-4 0.0 1.707084074113891 1.7509093347630093E-4 78-79 1.7509093347630093E-4 4.202182403431222E-4 0.0 2.02625733674784 1.7509093347630093E-4 80-81 1.7509093347630093E-4 4.902546137336425E-4 0.0 2.41989677338926 1.7509093347630093E-4 82-83 1.7509093347630093E-4 4.902546137336425E-4 0.0 2.816162574032824 1.7509093347630093E-4 84-85 1.7509093347630093E-4 4.902546137336425E-4 0.0 3.133444854585229 1.7509093347630093E-4 86-87 1.7509093347630093E-4 4.902546137336425E-4 0.0 3.378292015958488 1.7509093347630093E-4 88-89 1.7509093347630093E-4 4.902546137336425E-4 0.0 3.601515447047424 1.7509093347630093E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13800 0.0 26.238997 1 TACATGG 13875 0.0 25.715742 2 ACATGGG 13800 0.0 24.421162 3 CATGGGA 9070 0.0 21.69209 4 GAGTACT 11130 0.0 21.04832 12-13 GTATCAA 24805 0.0 20.653309 1 CATGGGG 4930 0.0 20.6224 4 AGTACTT 11700 0.0 20.283972 12-13 GTACTTT 11870 0.0 19.53817 14-15 GGTATCA 19615 0.0 18.628305 1 ATGGGAT 4065 0.0 18.410643 5 ATCAACG 28110 0.0 18.084034 3 TCAACGC 28190 0.0 18.032713 4 CAACGCA 28555 0.0 17.818695 5 AACGCAG 28565 0.0 17.80053 6 TATCAAC 28805 0.0 17.697968 2 TACTTTT 13370 0.0 17.48675 14-15 ACTTTTT 13720 0.0 17.331808 16-17 ATGGGAG 3660 0.0 16.461191 5 ACGCAGA 30560 0.0 16.453955 7 >>END_MODULE