FastQCFastQC Report
Fri 27 May 2016
SRR939666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890445
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58550.2025639650642029No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA41890.14492578132432896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38910.13461595013916544No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA32220.11147072509596273No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117450.030.2337911
TACATGG138200.025.4704152
ACATGGG140150.024.5433373
GTATCAA228850.024.0311781
GAGTACT96900.022.65416712-13
CATGGGA92750.022.259234
AGTACTT106900.020.88826212-13
GTACTTT107800.020.43098814-15
GAGTACG3958.13676E-620.0329131
CATGGGG54200.019.9608384
TATCAAC276500.019.770882
ATGGGAT41250.019.2256765
ATCAACG292850.018.6673833
TCAACGC297450.018.4082324
CAACGCA299600.018.2615625
AACGCAG306150.018.0096346
GTATAGG8853.6379788E-1217.8824861
ATGGGAG38450.017.8019685
ACGCAGA308700.017.6773627
GGTATCA185350.017.6638981
Produced by FastQC (version 0.11.5)