##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939666_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2890445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.49637754740187 2.0 2.0 31.0 2.0 33.0 2 22.686798399554394 16.0 16.0 31.0 16.0 34.0 3 27.09003423348308 28.0 25.0 31.0 16.0 34.0 4 32.30705756380073 35.0 32.0 35.0 25.0 37.0 5 32.909357209703 35.0 33.0 35.0 26.0 37.0 6 33.3003454485382 35.0 33.0 37.0 28.0 37.0 7 33.462471695534774 35.0 33.0 37.0 28.0 37.0 8 33.40005120318844 35.0 33.0 37.0 27.0 37.0 9 34.7462497989064 37.0 34.0 39.0 27.0 39.0 10-11 34.59133386035714 37.0 34.0 39.0 27.0 39.0 12-13 34.60402706157703 37.0 34.0 39.0 27.0 39.0 14-15 35.260656404117704 38.0 33.5 40.0 27.0 41.0 16-17 34.87466687655361 38.0 33.0 40.0 25.0 41.0 18-19 35.149282376935034 38.0 33.5 40.0 26.0 41.0 20-21 35.14644855722908 38.0 34.0 40.0 25.5 41.0 22-23 34.958510713748225 38.0 33.5 40.0 25.0 41.0 24-25 34.753947056595095 38.0 33.0 40.0 25.0 41.0 26-27 34.835406312868784 38.0 33.5 40.0 24.5 41.0 28-29 34.573278854986 38.0 33.0 40.0 24.0 41.0 30-31 34.43658571604026 38.0 33.0 40.0 24.0 41.0 32-33 34.11036778073965 38.0 33.0 40.0 23.0 41.0 34-35 33.837174898674775 38.0 32.5 40.0 21.0 41.0 36-37 33.53737953844477 38.0 32.0 40.0 19.0 41.0 38-39 33.319599750211474 37.0 31.0 40.0 18.0 41.0 40-41 32.91198621665522 37.0 31.0 40.0 15.5 41.0 42-43 32.65784922390843 37.0 31.0 40.0 15.0 41.0 44-45 32.30686831958401 37.0 30.0 39.5 14.0 41.0 46-47 31.944545390069695 36.5 30.0 39.0 9.0 40.5 48-49 31.548135667691305 36.0 30.0 39.0 8.5 40.0 50-51 30.874625706422368 35.5 28.5 38.5 8.0 40.0 52-53 30.895070482226785 35.5 29.0 38.5 7.0 40.0 54-55 31.020940893184267 36.0 29.0 39.0 4.0 40.0 56-57 30.754221754781703 35.5 28.5 39.0 2.0 40.0 58-59 30.356031683702682 35.0 28.0 39.0 2.0 40.0 60-61 29.767000755938966 34.5 27.0 38.0 2.0 40.0 62-63 29.352411133925745 34.0 26.5 38.0 2.0 40.0 64-65 28.75659630264544 34.0 26.0 38.0 2.0 39.5 66-67 28.16343867466774 33.5 25.5 37.0 2.0 39.0 68-69 27.834930953538297 33.0 25.0 36.5 2.0 39.0 70-71 27.30583681751426 33.0 23.5 36.0 2.0 39.0 72-73 26.713129985175293 32.0 22.0 35.5 2.0 38.0 74-75 26.15116167233765 32.0 21.5 35.0 2.0 38.0 76-77 25.502117321035342 31.0 19.5 35.0 2.0 37.0 78-79 24.841473371747256 31.0 17.5 34.5 2.0 36.0 80-81 24.11246987920545 30.0 11.5 34.0 2.0 36.0 82-83 23.431602227338697 30.0 7.0 34.0 2.0 35.0 84-85 22.737211744212395 29.0 3.5 34.0 2.0 35.0 86-87 22.022623160101645 29.0 2.0 34.0 2.0 35.0 88-89 21.189517358053862 28.5 2.0 33.0 2.0 35.0 90-91 20.179400057776572 26.5 2.0 32.5 2.0 34.0 92-93 19.093403264895198 25.5 2.0 32.0 2.0 34.0 94-95 18.087873839495302 24.5 2.0 32.0 2.0 34.0 96-97 17.201975993315905 21.5 2.0 31.5 2.0 34.0 98-99 16.14928964225232 10.0 2.0 31.0 2.0 34.0 100-101 14.614265969426853 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.945368832809706 1101 2 7.99631715457328 1101 3 3.7869827093927313 1101 4 2.4275547253035867 1101 5 1.792521409542843 1101 6 1.3952785057999009 1101 7 1.030743038739999 1101 8 1.0705757750456897 1101 9 1.1445935493946209 1101 10-11 1.0794481600020376 1101 12-13 0.7324159189715758 1101 14-15 0.842421271044266 1101 16-17 0.9222712816484062 1101 18-19 1.0642145779054104 1101 20-21 1.0038352422016246 1101 22-23 0.7820368020098698 1101 24-25 1.0355935120400446 1101 26-27 1.1630293587468472 1101 28-29 1.075805217644465 1101 30-31 1.1209470450654564 1101 32-33 1.1453755743453158 1101 34-35 1.1908426317405727 1101 36-37 1.01834599581219 1101 38-39 1.0226378323510943 1101 40-41 1.173969323206883 1101 42-43 1.1123765998941622 1101 44-45 0.8489315501872312 1101 46-47 0.7271894879994178 1101 48-49 0.7862110370651614 1101 50-51 0.8365675357974744 1101 52-53 0.8084560275136603 1101 54-55 0.8463163993262697 1101 56-57 0.42743290790624755 1101 58-59 0.4405627933161469 1101 60-61 0.5516328093458291 1101 62-63 0.23101075449162778 1101 64-65 0.47971021978932527 1101 66-67 0.26964163382608675 1101 68-69 0.09265017924293062 1101 70-71 -0.09408050140445923 1101 72-73 -0.07449806634680556 1101 74-75 -0.18928665612728324 1101 76-77 -0.31929970060704704 1101 78-79 -0.35461022508186346 1101 80-81 -0.3213996900509706 1101 82-83 -0.5774574919163911 1101 84-85 -0.6491037011024225 1101 86-87 -0.6938968394823632 1101 88-89 -0.807180320959997 1101 90-91 -0.726962861965756 1101 92-93 -0.6768369361020845 1101 94-95 -0.5896881077152969 1101 96-97 -1.2394666698075838 1101 98-99 -1.5149014922286774 1101 100-101 -1.3224555296203384 1102 1 14.851021692470049 1102 2 8.094124533968166 1102 3 3.84556306608372 1102 4 2.3440921236384753 1102 5 1.6403045885934588 1102 6 1.3100903911916149 1102 7 1.0357051505295587 1102 8 1.0445721738325346 1102 9 1.1602185789745718 1102 10-11 1.1696513936728579 1102 12-13 0.8025806996520899 1102 14-15 0.9830890692900027 1102 16-17 1.1871723517769013 1102 18-19 1.1159059453302476 1102 20-21 1.065496899049954 1102 22-23 1.0571468204980476 1102 24-25 1.0156718012580797 1102 26-27 1.046187635561175 1102 28-29 1.1415420610184484 1102 30-31 1.0838572882287494 1102 32-33 1.151474565932439 1102 34-35 1.2651803468822607 1102 36-37 1.2512140136342467 1102 38-39 1.0388176713882018 1102 40-41 1.0632528428292218 1102 42-43 0.902276026166156 1102 44-45 0.9197988034424824 1102 46-47 0.9568387653777606 1102 48-49 0.809863071578949 1102 50-51 0.7505788851594488 1102 52-53 0.8846950630539041 1102 54-55 0.7024594563642417 1102 56-57 0.5946418894585825 1102 58-59 0.6393275724064438 1102 60-61 0.8261069929738802 1102 62-63 0.49589863590577465 1102 64-65 0.5537009897086058 1102 66-67 0.43584555962045 1102 68-69 -0.058688487884143825 1102 70-71 0.0648476082388143 1102 72-73 -0.033462170452583706 1102 74-75 -0.12623639803865672 1102 76-77 -0.22719748138183604 1102 78-79 -0.2199257489811366 1102 80-81 -0.3204066240333141 1102 82-83 -0.23157657982276447 1102 84-85 -0.40771123033120205 1102 86-87 -0.5430761922408465 1102 88-89 -0.2750829026792303 1102 90-91 -0.3754760521314928 1102 92-93 -0.1443485260792201 1102 94-95 -0.2885431884813414 1102 96-97 -0.9863727804229079 1102 98-99 -1.240311828470217 1102 100-101 -1.3522470684227343 1103 1 14.795172601538905 1103 2 7.853586248766764 1103 3 3.726991741475185 1103 4 2.1743028044394777 1103 5 1.6493069780072673 1103 6 1.229007271126605 1103 7 0.833717934499866 1103 8 0.9428424565596174 1103 9 0.9428181884206381 1103 10-11 0.9050060457523799 1103 12-13 0.7077703972760219 1103 14-15 0.8054003116638881 1103 16-17 0.9808294890893592 1103 18-19 0.8973538451931873 1103 20-21 0.8687851327030884 1103 22-23 0.9014922787924604 1103 24-25 1.003114885906399 1103 26-27 0.9387254477314144 1103 28-29 1.1366706891067935 1103 30-31 0.9407505044767603 1103 32-33 0.9190969779061859 1103 34-35 0.9380973117202771 1103 36-37 0.8865907405012052 1103 38-39 0.8945462000187163 1103 40-41 0.7464683024578349 1103 42-43 0.8123630517704825 1103 44-45 0.8167470853690446 1103 46-47 0.7592547417019446 1103 48-49 0.6013953286653226 1103 50-51 0.6171671175996813 1103 52-53 0.7548967683918768 1103 54-55 0.5863327931745523 1103 56-57 0.7150061234513281 1103 58-59 0.6687646046817264 1103 60-61 0.5993009236202376 1103 62-63 0.30893634497948597 1103 64-65 0.2370852244290944 1103 66-67 0.36499574094905896 1103 68-69 0.3186729079216448 1103 70-71 -0.025677880863273828 1103 72-73 -0.03652607817512177 1103 74-75 -0.07291681090995539 1103 76-77 0.04533802627762995 1103 78-79 -0.054517244123470476 1103 80-81 -0.3283607036238365 1103 82-83 -0.25942574352793457 1103 84-85 -0.3858493675587553 1103 86-87 -0.7229547809099302 1103 88-89 -0.7824661306923772 1103 90-91 -0.7935927816668276 1103 92-93 -0.41990840090789305 1103 94-95 -0.5096541446107352 1103 96-97 -0.8924642695007421 1103 98-99 -1.1625529652004793 1103 100-101 -1.2742061451629443 1104 1 14.803669101320512 1104 2 7.985355246157834 1104 3 3.806404200599953 1104 4 2.193889152997478 1104 5 1.5848942237544748 1104 6 1.1915356919555578 1104 7 0.8767863215661791 1104 8 0.857840508714645 1104 9 0.8525735863122463 1104 10-11 0.7108366540702633 1104 12-13 0.6048979161937282 1104 14-15 0.8757946617329537 1104 16-17 1.0568951963931923 1104 18-19 0.6301255105416104 1104 20-21 0.6991557544788165 1104 22-23 0.738377969805228 1104 24-25 0.8836371444621562 1104 26-27 0.8319828938115279 1104 28-29 0.9305958918597454 1104 30-31 0.7576126442730029 1104 32-33 0.9097266040380063 1104 34-35 1.0052826757311877 1104 36-37 0.9966671786600827 1104 38-39 0.9075347844666481 1104 40-41 0.9783674513086069 1104 42-43 0.7804371052949008 1104 44-45 0.5850342213286268 1104 46-47 0.7396035830293144 1104 48-49 0.5820217083888544 1104 50-51 0.6588250288015622 1104 52-53 0.634378995782118 1104 54-55 0.6603965290231635 1104 56-57 0.6980304774160793 1104 58-59 0.7978268648902045 1104 60-61 0.6645603411555605 1104 62-63 0.5971354737615506 1104 64-65 0.7003823947415739 1104 66-67 0.5910541881034383 1104 68-69 0.5404403116213707 1104 70-71 0.3794276569190167 1104 72-73 0.38756309770937847 1104 74-75 0.39401255905073285 1104 76-77 0.17430844264425005 1104 78-79 -0.041772966655909016 1104 80-81 0.06727175573703548 1104 82-83 -0.21348304558556563 1104 84-85 -0.16950181298958 1104 86-87 -0.15411144667153565 1104 88-89 -0.18799476401351356 1104 90-91 -0.4206030167067887 1104 92-93 -0.15399220546230907 1104 94-95 -0.32979196939912825 1104 96-97 -0.8273392532687005 1104 98-99 -0.9544144557150656 1104 100-101 -1.01824491318051 1105 1 14.837498333834048 1105 2 7.907640753772327 1105 3 3.837162764834474 1105 4 2.2753507280583136 1105 5 1.705016562731032 1105 6 1.2617047127283811 1105 7 1.0256983757308689 1105 8 1.1045953301228337 1105 9 1.0183919741084395 1105 10-11 0.8553256137909386 1105 12-13 0.6835003750290767 1105 14-15 0.7500489953865497 1105 16-17 1.0725598839725663 1105 18-19 0.864717814418249 1105 20-21 0.8571916955507035 1105 22-23 0.7958019284031437 1105 24-25 0.9912088328845172 1105 26-27 0.9131810928927067 1105 28-29 1.1030240446870678 1105 30-31 1.0075168189001928 1105 32-33 0.5431899515806364 1105 34-35 0.747619234226768 1105 36-37 0.895495773871616 1105 38-39 0.8491659163702181 1105 40-41 0.9293789439112956 1105 42-43 0.8566629683444589 1105 44-45 0.7892600627513247 1105 46-47 0.6769378154876406 1105 48-49 0.6320634321136005 1105 50-51 0.7469096556048775 1105 52-53 0.854015696830885 1105 54-55 0.8261297898375126 1105 56-57 0.8085076992763192 1105 58-59 0.7009955557384551 1105 60-61 0.8188082465716597 1105 62-63 0.6549673516535677 1105 64-65 0.7034209678477055 1105 66-67 0.5407681269334894 1105 68-69 0.5532555460380735 1105 70-71 0.5583884428297203 1105 72-73 0.5664821253843897 1105 74-75 0.30839483165586046 1105 76-77 0.3162580299854518 1105 78-79 0.2439287143489075 1105 80-81 0.17018837868984704 1105 82-83 0.2551687132759213 1105 84-85 0.10402665209271333 1105 86-87 0.044621461210944346 1105 88-89 0.10385084186972904 1105 90-91 0.43732977116965444 1105 92-93 0.4808345542274388 1105 94-95 0.6478868731868168 1105 96-97 0.2241270820009298 1105 98-99 -0.0754367249335175 1105 100-101 0.10079176918633337 1106 1 14.631551911883733 1106 2 7.852419140013449 1106 3 3.5969519536767756 1106 4 2.213546836269714 1106 5 1.4901027339754407 1106 6 1.0054263692698413 1106 7 0.7185720459057023 1106 8 0.743134233747945 1106 9 0.9608155359007426 1106 10-11 0.8146612181661723 1106 12-13 0.5808141638542708 1106 14-15 0.77534062996628 1106 16-17 0.9248944758267612 1106 18-19 0.7872809008960999 1106 20-21 0.6511989258065398 1106 22-23 0.6984087244158133 1106 24-25 0.6618814641557336 1106 26-27 0.6939243867234666 1106 28-29 0.9246812991863678 1106 30-31 0.8463539527526294 1106 32-33 0.7176314606648049 1106 34-35 0.6731503621181574 1106 36-37 0.9196345070794578 1106 38-39 0.9615223273396722 1106 40-41 1.0253741380016024 1106 42-43 0.7966269774999262 1106 44-45 0.9548571649446487 1106 46-47 0.9762242377231658 1106 48-49 0.7905929413974242 1106 50-51 0.9061923165386787 1106 52-53 0.7169126835112429 1106 54-55 0.7084349151904696 1106 56-57 0.9350790677484113 1106 58-59 0.7893348964589109 1106 60-61 0.8312107379438416 1106 62-63 0.8629615439184732 1106 64-65 0.7551754101054868 1106 66-67 0.7777742555379206 1106 68-69 0.8435999636245626 1106 70-71 0.7420741085266478 1106 72-73 0.7788187494110872 1106 74-75 0.6234956559335458 1106 76-77 0.6495886894076008 1106 78-79 0.7113381935423106 1106 80-81 0.8424350523443316 1106 82-83 0.9331273068699417 1106 84-85 0.7205962557834233 1106 86-87 0.8284709485330417 1106 88-89 0.7857699435781775 1106 90-91 0.9324218071925863 1106 92-93 1.3693422622220766 1106 94-95 1.128005006582896 1106 96-97 0.5993129188281721 1106 98-99 0.4254510135793623 1106 100-101 0.5713774092137136 1107 1 14.750504140426727 1107 2 8.087912709675372 1107 3 3.7826634710416904 1107 4 2.248840074997375 1107 5 1.450095704667227 1107 6 1.0799542181188357 1107 7 0.8421923919251384 1107 8 0.7696972761160552 1107 9 0.6286782216706541 1107 10-11 0.7261316267795124 1107 12-13 0.45219734336348694 1107 14-15 0.7189888822523258 1107 16-17 0.8412365190935489 1107 18-19 0.8262451153665609 1107 20-21 0.6821177686153774 1107 22-23 0.7077741575316807 1107 24-25 0.9144882022000331 1107 26-27 0.7075496523886784 1107 28-29 0.956030230392237 1107 30-31 0.7488467146530766 1107 32-33 0.8436896086160814 1107 34-35 0.6588100948826465 1107 36-37 0.7438032239610166 1107 38-39 0.5191461253758121 1107 40-41 0.7087703008518815 1107 42-43 0.4676583436129178 1107 44-45 0.5337556579932041 1107 46-47 0.610365111410502 1107 48-49 0.7369595045963813 1107 50-51 0.6772060223847483 1107 52-53 0.6588804374297759 1107 54-55 0.691592686186393 1107 56-57 0.795277665279631 1107 58-59 0.8024796158216105 1107 60-61 0.8260699038398656 1107 62-63 0.5273079987721339 1107 64-65 0.5658403844024029 1107 66-67 0.5370223522744233 1107 68-69 0.7089586432107744 1107 70-71 0.5481039696021135 1107 72-73 0.6534879456427518 1107 74-75 0.6030456866867695 1107 76-77 0.272245604794044 1107 78-79 0.3977259447117589 1107 80-81 0.37345752960129985 1107 82-83 0.29566795593260053 1107 84-85 0.43569602324207324 1107 86-87 0.3232995025747627 1107 88-89 0.4202026357771267 1107 90-91 0.7677694512868776 1107 92-93 0.9510007157046729 1107 94-95 1.0569443444359834 1107 96-97 0.6317559383684319 1107 98-99 0.3246858694654904 1107 100-101 0.057272575955343186 1108 1 14.348099157741323 1108 2 7.504320686742581 1108 3 3.441826045102591 1108 4 -0.04180556994475637 1108 5 0.39629309533821555 1108 6 0.6207851484217244 1108 7 0.6245419380824941 1108 8 0.6929611309886354 1108 9 0.7878428189625097 1108 10-11 0.7453990148522465 1108 12-13 0.3354405719289488 1108 14-15 0.35898629464642795 1108 16-17 0.6788478500387569 1108 18-19 0.5116888205626466 1108 20-21 0.434427765931936 1108 22-23 -0.34829500289136206 1108 24-25 0.2472628930716425 1108 26-27 0.21921246071843825 1108 28-29 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3 -3.196211162114267 2312 4 -1.2070427922397258 2312 5 -1.8491118510749835 2312 6 -0.5730022740549785 2312 7 -0.05464094747069481 2312 8 0.1450971633034044 2312 9 0.11057644036707615 2312 10-11 0.2795792518166209 2312 12-13 -0.4841965317251038 2312 14-15 0.2337530303549613 2312 16-17 -0.1529257918402962 2312 18-19 0.0673509479250427 2312 20-21 0.12430569277136527 2312 22-23 0.33859785524347075 2312 24-25 0.492880470575102 2312 26-27 0.5284489429956949 2312 28-29 -0.2676517188917771 2312 30-31 0.3165417220668658 2312 32-33 0.4657291407431927 2312 34-35 -0.4054906124661173 2312 36-37 0.2293433032665675 2312 38-39 0.5201480416277064 2312 40-41 0.6681765844305048 2312 42-43 0.5087989378604405 2312 44-45 0.5771414825792434 2312 46-47 0.4640895974179955 2312 48-49 0.40332164323489295 2312 50-51 0.4915457852777614 2312 52-53 0.3680143342652187 2312 54-55 0.34350742996248584 2312 56-57 0.2867543877243257 2312 58-59 0.3954474433723689 2312 60-61 0.7329890932842567 2312 62-63 0.42249113495388 2312 64-65 0.46734661069287853 2312 66-67 0.34688418032240875 2312 68-69 0.8181623039202535 2312 70-71 0.893041684651859 2312 72-73 0.49402914375561835 2312 74-75 0.586096432239323 2312 76-77 0.6163886810602293 2312 78-79 0.6418776587917066 2312 80-81 0.567360716142904 2312 82-83 0.6167121453644668 2312 84-85 0.7108625491684926 2312 86-87 0.7685425272645823 2312 88-89 1.222090218539055 2312 90-91 1.3755567911178126 2312 92-93 1.9656119484499648 2312 94-95 2.0771931535550294 2312 96-97 1.7089114060895128 2312 98-99 1.6710244234654041 2312 100-101 1.634770750440584 2313 1 -14.165861881219715 2313 2 -7.383676285610219 2313 3 -3.594977795228857 2313 4 -1.0573620993049708 2313 5 -0.475406370839778 2313 6 -0.9518664331255664 2313 7 -0.2130686108777482 2313 8 -0.0026388212064105687 2313 9 -2.4030861906781595 2313 10-11 -0.45241092061106514 2313 12-13 -0.6988405610484918 2313 14-15 -0.9011672787740181 2313 16-17 -0.8759824423682261 2313 18-19 -0.26466963693078327 2313 20-21 -1.1800312413184812 2313 22-23 -2.026480008070237 2313 24-25 -1.18509010668933 2313 26-27 -0.38388191423177176 2313 28-29 0.03788287442517202 2313 30-31 -1.2307430432279105 2313 32-33 -0.2981687297307971 2313 34-35 -1.2106619436213748 2313 36-37 -1.2045357667972922 2313 38-39 -0.7114459726066258 2313 40-41 -0.31042084499298284 2313 42-43 -0.06299552660903629 2313 44-45 -0.29043929076804176 2313 46-47 -0.8287217761740848 2313 48-49 -0.46787905254897666 2313 50-51 -1.191183844955848 2313 52-53 -0.4680607099241776 2313 54-55 -1.0673122248072495 2313 56-57 -0.6460939400138699 2313 58-59 -0.2674359453500088 2313 60-61 -1.7403690573402066 2313 62-63 -0.42423374175628936 2313 64-65 -0.43601187732677715 2313 66-67 -1.5990924756788196 2313 68-69 -0.6038551225416491 2313 70-71 -0.09600684214950306 2313 72-73 -0.9402842757634424 2313 74-75 -0.3145214227144848 2313 76-77 0.34118422894317035 2313 78-79 0.3333058995194307 2313 80-81 0.18741886058178991 2313 82-83 0.3138141549277478 2313 84-85 0.36200973969743444 2313 86-87 0.1260139095914461 2313 88-89 0.5741554051654596 2313 90-91 1.0321465879121838 2313 92-93 1.424852546839091 2313 94-95 1.4573830883065462 2313 96-97 0.9705778290818721 2313 98-99 0.9012380227411327 2313 100-101 1.0113999474962476 2314 1 -14.165861881219715 2314 2 -7.2203722528702095 2314 3 -3.35734645317595 2314 4 -1.3428008139116443 2314 5 -1.280034830743105 2314 6 -6.12931493645025 2314 7 -1.9444197414217257 2314 8 -4.16356640436134 2314 9 -1.4853560536814925 2314 10-11 -0.3542500875866281 2314 12-13 -2.7563426602519314 2314 14-15 -2.903396405883626 2314 16-17 -2.744272556065617 2314 18-19 -1.1395926661380784 2314 20-21 -1.6517901468581115 2314 22-23 -0.6329107570508938 2314 24-25 -2.5118226114361804 2314 26-27 -4.426681457324069 2314 28-29 -1.6755232817252974 2314 30-31 -1.7473229680348226 2314 32-33 -3.9732328405384756 2314 34-35 -2.490746176523267 2314 36-37 -0.6894992293104352 2314 38-39 -1.2194042230434832 2314 40-41 -2.1811808322842907 2314 42-43 -1.1218078313452935 2314 44-45 -2.2058682469154896 2314 46-47 -2.102195787657294 2314 48-49 -1.2702369686262251 2314 50-51 -2.172277586681961 2314 52-53 -0.8883295592581071 2314 54-55 -0.44999039233503524 2314 56-57 -0.9622373817997456 2314 58-59 -1.4610449110289778 2314 60-61 -0.6222769495886595 2314 62-63 -2.8888735108817336 2314 64-65 -1.1537885372104313 2314 66-67 -2.1620585555771505 2314 68-69 -1.8231169215779062 2314 70-71 -2.4630748790349664 2314 72-73 -2.463394992669784 2314 74-75 -1.8317853852541006 2314 76-77 -0.7645590781910983 2314 78-79 -0.256938478019471 2314 80-81 -0.007101083742039549 2314 82-83 -0.2899315048273081 2314 84-85 0.16074530058190106 2314 86-87 0.3226742849517166 2314 88-89 0.5789204078509265 2314 90-91 1.0992359950668735 2314 92-93 1.4733447135952744 2314 94-95 1.3250271768740234 2314 96-97 1.2732655659514478 2314 98-99 1.2655277355095826 2314 100-101 1.3208189409376558 2315 1 -14.165861881219715 2315 2 -7.3328138184961595 2315 3 -4.435658943240366 2315 4 -2.8485609145696564 2315 5 -3.3536540620457025 2315 6 -2.705495245978149 2315 7 -3.2503178409680835 2315 8 -3.4447100137763833 2315 9 -2.2983438836978323 2315 10-11 -4.457489566392788 2315 12-13 -4.183586579675918 2315 14-15 -3.2634166491029077 2315 16-17 -4.385223228227691 2315 18-19 -1.3867883423232357 2315 20-21 -3.232899961202257 2315 22-23 -3.926958361781793 2315 24-25 -4.803816768656446 2315 26-27 -4.299310212128379 2315 28-29 -2.4305322242186236 2315 30-31 -2.992048841811691 2315 32-33 -4.170557721565167 2315 34-35 -3.6201796538810385 2315 36-37 -4.784405644116603 2315 38-39 -3.3441294560575408 2315 40-41 -4.272453126038524 2315 42-43 -2.968016300613673 2315 44-45 -1.6802845702447797 2315 46-47 -1.0559554023878448 2315 48-49 -0.6375332990659182 2315 50-51 -0.6277403433300144 2315 52-53 -0.30449786528793155 2315 54-55 -1.8579045083209955 2315 56-57 -3.775476067638433 2315 58-59 -3.0741561301657256 2315 60-61 -4.081345641241192 2315 62-63 -1.9933510148456328 2315 64-65 -2.6746811630263707 2315 66-67 -1.3802376824184996 2315 68-69 -1.1502874069529199 2315 70-71 -1.6657141923326542 2315 72-73 -1.9498108210520826 2315 74-75 -2.4280616889433553 2315 76-77 -2.1920343580879447 2315 78-79 -1.4092781454466596 2315 80-81 -1.0921266917056904 2315 82-83 -0.7067098978543065 2315 84-85 -0.473483656936466 2315 86-87 -1.1282491087536286 2315 88-89 -1.7327748570058432 2315 90-91 -1.3693582652767482 2315 92-93 -0.3886185817821044 2315 94-95 -0.21510407051642133 2315 96-97 -1.242891756915217 2315 98-99 -0.6391054775758072 2315 100-101 -0.11671189929593595 2316 1 -14.165861881219715 2316 2 -7.289511157089262 2316 3 -5.183256304979757 2316 4 -2.768788046927238 2316 5 -2.017358118313002 2316 6 -1.2584302307936568 2316 7 -4.818581155040601 2316 8 -2.808451645424139 2316 9 -1.2274378463181534 2316 10-11 -4.323455441502215 2316 12-13 -3.604618355629974 2316 14-15 -1.7293851312250013 2316 16-17 -3.152583556392649 2316 18-19 -2.357430573556748 2316 20-21 -3.2385256320214637 2316 22-23 -3.0130970926934566 2316 24-25 -3.3162442079354584 2316 26-27 -4.147097243091274 2316 28-29 -3.6662494382960915 2316 30-31 -2.530675762639163 2316 32-33 -2.9263029483558114 2316 34-35 -4.005769273698029 2316 36-37 -2.203845286658961 2316 38-39 -3.1272858821581906 2316 40-41 -2.640123516720191 2316 42-43 -6.560123506405731 2316 44-45 -3.3322288268827265 2316 46-47 -4.550506429093701 2316 48-49 -4.162881065955897 2316 50-51 -3.228847445656257 2316 52-53 -4.53840003731872 2316 54-55 -4.641652264638086 2316 56-57 -4.452432709849571 2316 58-59 -3.2518764550972676 2316 60-61 -3.0599360928581625 2316 62-63 -2.06557467987637 2316 64-65 -2.003337744959392 2316 66-67 -2.320347857670402 2316 68-69 -2.4931849313499868 2316 70-71 -2.645647617557149 2316 72-73 -3.607339244505525 2316 74-75 -4.23164052894402 2316 76-77 -3.9279751144493034 2316 78-79 -4.055336708908452 2316 80-81 -4.003145275994616 2316 82-83 -3.4903893558708567 2316 84-85 -3.305953266455756 2316 86-87 -3.38393355050939 2316 88-89 -3.035919783283962 2316 90-91 -2.1540033589739593 2316 92-93 -2.12176757156017 2316 94-95 -1.458467526010324 2316 96-97 -1.3935321903962468 2316 98-99 -1.6693528894141991 2316 100-101 -1.7451212133193916 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19256.0 3 5983.0 4 6644.0 5 7926.0 6 9485.0 7 11740.0 8 14556.0 9 17901.0 10 21113.0 11 25253.0 12 29124.0 13 32422.0 14 35245.0 15 39700.0 16 44130.0 17 46211.0 18 42345.0 19 42532.0 20 45443.0 21 49933.0 22 55165.0 23 61265.0 24 68331.0 25 75912.0 26 85132.0 27 95559.0 28 108348.0 29 122262.0 30 138508.0 31 158314.0 32 180462.0 33 207683.0 34 240206.0 35 277326.0 36 284862.0 37 164334.0 38 19735.0 39 99.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.50324131321538 18.937252029940765 13.755642145112605 22.80386451173124 2 15.018685142195457 21.133913879362407 41.01870037841222 22.82870060002992 3 18.85011946963656 26.93997005811718 30.16980691572974 24.040103556516517 4 12.224427640742176 16.79969661822218 38.81411418552461 32.161761555511035 5 12.848784847309206 38.66485617691071 34.61373524358477 13.872623732195311 6 29.857027428946818 37.3220985202422 18.645405571717045 14.175468479093933 7 26.851523628446795 33.80299503760863 22.24967748440958 17.095803849535 8 25.36314165478457 34.66644952607333 21.081186904343216 18.88922191479888 9 27.236981466512884 16.47257083403637 20.798194498194743 35.492253201256005 10-11 24.212959097810728 26.686382599724872 28.586497853753134 20.514160448711262 12-13 26.139640239701617 24.42835447055153 28.34333187185079 21.088673417896064 14-15 23.240977026130373 25.400167353578613 27.18426737765951 24.174588242631508 16-17 21.66920656049639 28.554256168546626 27.745497614486215 22.031039656470767 18-19 21.421062497422984 27.596049599336403 30.057929646611488 20.924958256629132 20-21 22.736862317090033 26.71838060799984 29.554340709634687 20.990416365275433 22-23 22.13200153290627 26.71562352834325 29.222888973665178 21.929485965085306 24-25 22.049821893768726 26.73647940864501 29.76449338806839 21.449205309517875 26-27 22.12291899367977 26.681781452881374 29.802075237725894 21.39322431571296 28-29 21.7370177514916 26.776843715832594 30.091221715001527 21.39491681767428 30-31 21.84881861094134 27.23156612614487 29.667563924179575 21.25205133873422 32-33 21.545640772437245 27.26858539298338 29.542857202297036 21.64291663228234 34-35 22.086749804901 26.83848090768619 29.440274743771134 21.63449454364168 36-37 22.274191680988793 26.697735105278888 29.59331173517599 21.43476147855633 38-39 22.22979993396703 26.73011146746576 29.745703740741735 21.29438485782547 40-41 22.052443713435725 26.75312250587325 29.928793087461365 21.265640693229667 42-43 21.711992449467427 27.1651933758149 29.296648688630476 21.8261654860872 44-45 21.818570711967773 27.43558206070153 28.905471360541128 21.84037586678957 46-47 21.996928831379943 27.19811603552597 28.40449548071487 22.40045965237922 48-49 22.150086023067672 27.229651509049024 28.478776150865198 22.141486317018106 50-51 22.465256458119004 27.550271430263308 28.238735171560194 21.745736940057494 52-53 22.12608151788186 27.648040727720787 28.492372224615764 21.733505529781592 54-55 21.77070832495127 27.454546085171028 28.581182322051653 22.19356326782605 56-57 22.0673031993718 28.06319299826739 27.802582044076907 22.066921758283904 58-59 21.938162118244588 27.46554043198788 28.29108306847304 22.305214381294494 60-61 22.095999448125628 27.32088392109514 28.4376148835526 22.145501747226632 62-63 22.49139524366753 27.216302176058242 28.3892045213256 21.903098058948625 64-65 22.18392354468588 27.274745600148638 28.54411914062297 21.997211714542516 66-67 21.769331113336655 27.622405742872107 28.394707367067994 22.213555776723247 68-69 21.766629635855992 27.726643292772753 28.42853602358418 22.078191047787076 70-71 21.901303974253665 27.380599316128084 28.155228905730407 22.562867803887844 72-73 21.799609402427322 27.471873247360257 27.763824562224727 22.964692787987694 74-75 21.847964051699872 27.69930913876726 27.81468679726153 22.63804001227134 76-77 22.017294254833423 27.20883102324746 28.205742738681316 22.5681319832378 78-79 21.831411873363642 27.320656703352498 28.230634850004037 22.617296573279816 80-81 21.73121044955492 27.934783928190527 27.7903256294688 22.543679992785755 82-83 21.899946383374825 27.595308233169202 27.773498543750264 22.73124683970571 84-85 21.913205556544636 27.26959587237622 27.622024186671553 23.195174384407586 86-87 21.96944190067161 27.277458790013277 28.014295675825057 22.73880363349005 88-89 22.271501580239388 26.903217987734312 28.005770660791285 22.819509771235012 90-91 21.64968219668701 27.180196602065877 28.30809715862255 22.86202404262456 92-93 21.556600560031022 27.36295699708543 28.165143451393572 22.915298991489976 94-95 21.541880587929768 27.28669076631864 27.892777648194215 23.278650997557374 96-97 21.639012855115027 26.995793600006383 27.631567306823307 23.733626238055283 98-99 21.76311837835165 26.974850640163154 27.538544683900195 23.723486297584998 100-101 22.068954150564497 26.986602127871823 27.714624022484486 23.229819699079194 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4979.0 1 3389.5 2 1673.5 3 1378.0 4 1149.5 5 1212.5 6 1715.5 7 3499.0 8 4732.5 9 4224.5 10 3600.5 11 3354.0 12 3452.5 13 3589.5 14 3761.5 15 3980.0 16 4252.5 17 4591.5 18 5036.5 19 5557.0 20 6200.5 21 7142.0 22 8484.5 23 10050.0 24 11714.5 25 14301.0 26 18225.0 27 22365.5 28 26327.0 29 31436.0 30 36963.0 31 43019.0 32 49790.5 33 56380.5 34 62730.0 35 69495.5 36 77752.0 37 83866.0 38 87592.0 39 89980.0 40 90884.0 41 91489.5 42 91891.0 43 94469.5 44 97167.0 45 99158.0 46 103223.5 47 115408.0 48 127024.0 49 119363.0 50 105377.5 51 98564.0 52 94176.0 53 90039.0 54 84864.0 55 78018.5 56 71004.0 57 65012.0 58 58892.0 59 51914.5 60 43522.5 61 35006.5 62 27781.0 63 21202.5 64 15731.0 65 11327.0 66 7983.0 67 5736.5 68 3956.0 69 2785.0 70 2047.5 71 1415.5 72 943.5 73 721.5 74 569.5 75 407.0 76 282.5 77 181.0 78 122.5 79 97.0 80 61.5 81 40.0 82 31.0 83 22.0 84 17.5 85 11.0 86 8.0 87 5.0 88 6.0 89 6.0 90 5.0 91 4.0 92 1.5 93 1.0 94 1.0 95 4.5 96 3.5 97 2.0 98 2.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.24060655020247 2 0.08957098301472612 3 0.12174595953218276 4 0.19481429330085853 5 0.12333740998358383 6 0.14866223021022715 7 0.15700004670561107 8 0.15395553279858293 9 0.1437840886091934 10-11 0.13070651750854972 12-13 0.14207154953649004 14-15 0.1501498904147977 16-17 0.14425114471993067 18-19 0.15077263189578075 20-21 0.1803874489914183 22-23 0.19818747632284994 24-25 0.22460209414121354 26-27 0.21773463947592844 28-29 0.21479391581573076 30-31 0.23032785609136308 32-33 0.22069266151059785 34-35 0.2079956546483327 36-37 0.19481429330085853 38-39 0.19106054604048858 40-41 0.1917524810193586 42-43 0.18510990522220627 44-45 0.20086872436597134 46-47 0.22579568197976435 48-49 0.22922076012517104 50-51 0.23953059131033458 52-53 0.2660835961244722 54-55 0.2527292510322805 56-57 0.22987809835509757 58-59 0.18798143538451692 60-61 0.19825666982073695 62-63 0.20233908619607016 64-65 0.19090486067024282 66-67 0.19913888691879622 68-69 0.1888982492315197 70-71 0.20206231220452217 72-73 0.22150568511077012 74-75 0.25271195265780877 76-77 0.2522448965470715 78-79 0.24681320696294168 80-81 0.25117239732982294 82-83 0.24255780684289097 84-85 0.2134792393558777 86-87 0.22474048113698755 88-89 0.2264876169586344 90-91 0.21424036783263475 92-93 0.21244133688757266 94-95 0.25177784043633417 96-97 0.2785557241186046 98-99 0.296234662828734 100-101 0.2926020041896663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2890445.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.1268543857333 #Duplication Level Percentage of deduplicated Percentage of total 1 78.56904489207885 50.38385701020482 2 12.202661279142498 15.65036565931994 3 4.210050089179682 8.099318070764069 4 1.8367852322970133 4.7114903651750355 5 0.9834490866472305 3.153274818760468 6 0.5927625830509496 2.280719990917163 7 0.3801647262893372 1.7065137631743932 8 0.26004998911157684 1.3340950227815693 9 0.18916283086215124 1.0917375578901243 >10 0.7375449384227151 7.869585079965111 >50 0.02641192051466444 1.163924017515853 >100 0.010512310488093818 1.1288968577844958 >500 7.304983905968377E-4 0.3324012181675309 >1k 6.087486588205338E-4 0.8652581327765654 >5k 6.087486588205338E-5 0.22856243480292313 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5855 0.2025639650642029 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4189 0.14492578132432896 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3891 0.13461595013916544 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3222 0.11147072509596273 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 1.729837447175089E-5 0.0 3.459674894350178E-5 0.0 14-15 0.0 3.459674894350178E-5 0.0 6.919349788700356E-5 0.0 16-17 0.0 3.459674894350178E-5 0.0 1.0379024683050534E-4 0.0 18-19 0.0 8.649187235875444E-5 0.0 1.7298374471750889E-4 1.729837447175089E-5 20-21 0.0 1.3838699577400711E-4 0.0 2.940723660197651E-4 3.459674894350178E-5 22-23 0.0 1.3838699577400711E-4 0.0 7.7842685122879E-4 5.189512341525267E-5 24-25 0.0 1.3838699577400711E-4 0.0 0.0014703618300988255 6.919349788700356E-5 26-27 0.0 1.3838699577400711E-4 0.0 0.0022660870557993664 6.919349788700356E-5 28-29 0.0 1.3838699577400711E-4 0.0 0.005051125345751259 6.919349788700356E-5 30-31 0.0 1.7298374471750889E-4 0.0 0.013008377602756669 6.919349788700356E-5 32-33 0.0 1.7298374471750889E-4 0.0 0.029545623597750523 6.919349788700356E-5 34-35 0.0 1.7298374471750889E-4 0.0 0.05289842913461422 6.919349788700356E-5 36-37 0.0 1.7298374471750889E-4 0.0 0.09213114243654524 6.919349788700356E-5 38-39 0.0 1.7298374471750889E-4 0.0 0.14745134399720458 6.919349788700356E-5 40-41 0.0 1.7298374471750889E-4 0.0 0.21245863526204442 6.919349788700356E-5 42-43 0.0 1.7298374471750889E-4 0.0 0.2758744760754832 6.919349788700356E-5 44-45 0.0 2.0758049366101069E-4 0.0 0.3516413562617521 6.919349788700356E-5 46-47 0.0 2.0758049366101069E-4 0.0 0.4405722994210234 8.649187235875444E-5 48-49 0.0 2.0758049366101069E-4 0.0 0.5405742022422153 1.0379024683050534E-4 50-51 0.0 2.0758049366101069E-4 0.0 0.6475646483499946 1.0379024683050534E-4 52-53 0.0 2.0758049366101069E-4 0.0 0.7518911447891241 1.2108862130225623E-4 54-55 0.0 2.4217724260451246E-4 0.0 0.8646246512215248 1.7298374471750889E-4 56-57 0.0 2.4217724260451246E-4 0.0 1.0000536249608625 1.7298374471750889E-4 58-59 0.0 2.5947561707626336E-4 0.0 1.1566385106791515 1.7298374471750889E-4 60-61 0.0 2.7677399154801423E-4 0.0 1.3451043005488774 1.7298374471750889E-4 62-63 0.0 2.7677399154801423E-4 0.0 1.532134325337448 1.7298374471750889E-4 64-65 3.459674894350178E-5 2.7677399154801423E-4 0.0 1.711639557230807 1.7298374471750889E-4 66-67 3.459674894350178E-5 2.7677399154801423E-4 0.0 1.9181302533000975 1.7298374471750889E-4 68-69 3.459674894350178E-5 2.7677399154801423E-4 0.0 2.155585039673822 2.0758049366101069E-4 70-71 3.459674894350178E-5 2.940723660197651E-4 0.0 2.421582143925935 2.0758049366101069E-4 72-73 3.459674894350178E-5 3.1137074049151603E-4 0.0 2.697750692367438 2.5947561707626336E-4 74-75 3.459674894350178E-5 3.1137074049151603E-4 0.0 2.9743689985452066 2.7677399154801423E-4 76-77 3.459674894350178E-5 3.1137074049151603E-4 0.0 3.2725064825658334 2.7677399154801423E-4 78-79 3.459674894350178E-5 3.1137074049151603E-4 0.0 3.6091328497861057 2.7677399154801423E-4 80-81 3.459674894350178E-5 3.4596748943501777E-4 0.0 3.979854313090199 2.940723660197651E-4 82-83 3.459674894350178E-5 3.4596748943501777E-4 0.0 4.366784353274323 3.1137074049151603E-4 84-85 3.459674894350178E-5 3.4596748943501777E-4 0.0 4.766324908448353 3.1137074049151603E-4 86-87 3.459674894350178E-5 3.4596748943501777E-4 0.0 5.193560161151656 3.1137074049151603E-4 88-89 5.189512341525267E-5 3.4596748943501777E-4 0.0 5.630551697057028 3.1137074049151603E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11745 0.0 30.233791 1 TACATGG 13820 0.0 25.470415 2 ACATGGG 14015 0.0 24.543337 3 GTATCAA 22885 0.0 24.031178 1 GAGTACT 9690 0.0 22.654167 12-13 CATGGGA 9275 0.0 22.25923 4 AGTACTT 10690 0.0 20.888262 12-13 GTACTTT 10780 0.0 20.430988 14-15 GAGTACG 395 8.13676E-6 20.032913 1 CATGGGG 5420 0.0 19.960838 4 TATCAAC 27650 0.0 19.77088 2 ATGGGAT 4125 0.0 19.225676 5 ATCAACG 29285 0.0 18.667383 3 TCAACGC 29745 0.0 18.408232 4 CAACGCA 29960 0.0 18.261562 5 AACGCAG 30615 0.0 18.009634 6 GTATAGG 885 3.6379788E-12 17.882486 1 ATGGGAG 3845 0.0 17.801968 5 ACGCAGA 30870 0.0 17.677362 7 GGTATCA 18535 0.0 17.663898 1 >>END_MODULE