FastQCFastQC Report
Fri 27 May 2016
SRR939666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2890445
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139780.4835933567322679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104010.35984078576136197No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA95720.331160080887199No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA80490.2784692322462458No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78230.27065036698501443No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT51810.17924575627628273No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG39870.1379372380377416No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA39370.13620740059056652No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT33940.11742136591424504No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC31380.10856459818470857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG141100.025.2954621
TACATGG140600.024.7737082
ACATGGG141950.023.638323
GTATCAA242350.021.7794341
CATGGGA92800.020.9014424
GAGTACT106200.020.60512412-13
AGTACTT112800.020.38202312-13
ATCAACG272250.019.293443
TCAACGC273050.019.2541984
TATCAAC278150.018.90282
CAACGCA277900.018.8842015
CATGGGG54300.018.8600254
GTACTTT114450.018.8313814-15
AACGCAG279650.018.7692576
ATGGGAG36300.018.5913895
TACTTTT129000.017.5665114-15
GGTATCA185500.017.510251
ATGGGAT40550.017.457535
ACGCAGA296050.017.3949957
CGCAGAG296350.017.2341678
Produced by FastQC (version 0.11.5)