##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939666_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2890445 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.478689959504507 31.0 31.0 34.0 30.0 34.0 2 31.671972654729636 31.0 31.0 34.0 30.0 34.0 3 31.722815345042026 31.0 31.0 34.0 30.0 34.0 4 35.52398506112381 37.0 35.0 37.0 33.0 37.0 5 35.28245996723688 37.0 35.0 37.0 33.0 37.0 6 35.292271605237254 37.0 35.0 37.0 32.0 37.0 7 35.1994433383095 37.0 35.0 37.0 32.0 37.0 8 35.16675909764759 37.0 35.0 37.0 32.0 37.0 9 36.75894784367113 39.0 35.0 39.0 32.0 39.0 10-11 36.63722471799325 39.0 35.0 39.0 32.0 39.0 12-13 36.57081971807109 39.0 35.0 39.0 32.0 39.0 14-15 37.60855370020879 39.0 36.0 41.0 32.0 41.0 16-17 37.57169172912822 39.0 36.0 41.0 32.0 41.0 18-19 37.575396867956314 39.0 36.0 41.0 32.0 41.0 20-21 37.51969938884843 39.0 36.0 41.0 32.0 41.0 22-23 37.380872149444116 39.0 36.0 41.0 32.0 41.0 24-25 37.250074988453335 39.0 36.0 41.0 31.0 41.0 26-27 36.943884765148624 39.0 36.0 40.0 31.0 41.0 28-29 36.74241492226976 39.0 35.5 40.0 30.0 41.0 30-31 36.500750749452074 38.0 35.0 40.0 30.0 41.0 32-33 36.317936165538526 38.0 35.0 40.0 30.0 41.0 34-35 36.2789684633335 38.0 35.0 40.0 30.0 41.0 36-37 36.17569993547706 38.0 35.0 40.0 30.0 41.0 38-39 36.19714559522842 38.0 35.0 40.0 30.0 41.0 40-41 36.0696863285757 38.0 35.0 40.0 30.0 41.0 42-43 35.88644672360138 38.0 35.0 40.0 29.0 41.0 44-45 35.659874344607836 38.0 35.0 40.0 28.0 41.0 46-47 35.34118777558473 38.0 34.0 40.0 27.0 41.0 48-49 35.02879141447078 38.0 34.0 40.0 26.0 41.0 50-51 34.784658936599726 38.0 34.0 40.0 25.5 41.0 52-53 34.52613940068052 38.0 33.0 40.0 24.0 41.0 54-55 34.245397507996174 38.0 33.0 40.0 24.0 41.0 56-57 33.40294747002624 37.0 32.0 40.0 20.0 41.0 58-59 30.32755561859852 35.5 26.0 39.0 7.5 40.5 60-61 30.02069456433179 34.5 25.5 38.5 10.0 40.0 62-63 31.87209426230217 35.0 30.5 39.0 16.5 40.0 64-65 31.91979470289177 35.5 31.0 39.0 11.0 40.0 66-67 27.002443222410392 31.5 18.5 37.0 2.0 39.5 68-69 19.652339345671688 19.5 8.0 32.0 2.0 37.0 70-71 17.728969414744096 15.5 7.5 29.0 2.0 35.0 72-73 19.428200501998827 20.0 7.5 31.0 2.0 35.5 74-75 20.820574167645468 23.5 9.0 32.5 2.0 36.0 76-77 22.570716273791753 27.0 11.0 33.0 2.0 35.5 78-79 24.678714004245023 30.5 13.0 34.5 2.0 36.5 80-81 26.301281982532103 32.0 22.0 35.0 2.0 36.5 82-83 27.51266708067443 33.0 26.5 35.0 2.0 36.0 84-85 27.584387871071755 34.0 27.0 35.0 2.0 36.0 86-87 27.305334126752108 33.0 26.0 35.0 2.0 36.0 88-89 26.85934051677164 33.0 25.5 35.0 2.0 35.0 90-91 24.981813181015383 32.0 15.0 34.5 2.0 35.0 92-93 23.314989560431005 31.0 2.0 34.0 2.0 35.0 94-95 19.66193302415372 27.0 2.0 34.0 2.0 35.0 96-97 17.000705254727215 17.5 2.0 33.0 2.0 35.0 98-99 17.259826601094296 20.0 2.0 33.0 2.0 35.0 100-101 16.48275819121277 10.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27335352760743703 1101 2 0.24215289077732294 1101 3 0.2134030352273193 1101 4 0.22201844159850737 1101 5 0.31090119646166414 1101 6 0.3059917033888553 1101 7 0.3179791298116541 1101 8 0.3251210959227748 1101 9 0.32172155718964746 1101 10-11 0.40348915176178224 1101 12-13 0.31744756416255626 1101 14-15 0.44028352293921813 1101 16-17 0.4681687219166122 1101 18-19 0.4765879372864532 1101 20-21 0.4167990995287454 1101 22-23 0.3573256632548265 1101 24-25 0.3804912253695534 1101 26-27 0.43272588501610443 1101 28-29 0.4290974676700685 1101 30-31 0.18769608391443882 1101 32-33 0.4129816469586274 1101 34-35 0.2850697159835178 1101 36-37 0.29421756044221326 1101 38-39 0.278852140984462 1101 40-41 0.25199776955063413 1101 42-43 0.5259546184652137 1101 44-45 0.35278469406924273 1101 46-47 0.20101184067139144 1101 48-49 0.02473230029723794 1101 50-51 0.2214458333316358 1101 52-53 0.044325548121328495 1101 54-55 0.31191123457927006 1101 56-57 -3.371593178198907 1101 58-59 -10.201249761080785 1101 60-61 -11.813290466919167 1101 62-63 -3.9394374426237455 1101 64-65 -0.9047895327837914 1101 66-67 -2.0403037551517187 1101 68-69 -4.7564896988117 1101 70-71 -4.434675376806256 1101 72-73 -3.5238157312901173 1101 74-75 -6.1560413652064625 1101 76-77 -4.8842485981781 1101 78-79 1.168449529874593 1101 80-81 1.8320804889953415 1101 82-83 0.4291928732473309 1101 84-85 0.18047003073765566 1101 86-87 0.2078202757707217 1101 88-89 -0.007218800348756105 1101 90-91 1.1354843922358597 1101 92-93 0.6927942880139248 1101 94-95 -8.364780192997923 1101 96-97 -9.414506324244869 1101 98-99 -8.695410275803418 1101 100-101 -9.89356523783729 1102 1 0.3886116585129358 1102 2 0.41605927816633326 1102 3 0.3787741157969897 1102 4 0.23442845386464484 1102 5 0.39655716335786906 1102 6 0.4281580702489123 1102 7 0.4842681196583598 1102 8 0.3974261714575107 1102 9 0.3181518959780547 1102 10-11 0.3858186093800029 1102 12-13 0.3738254055574757 1102 14-15 0.49979024482937007 1102 16-17 0.3863646799606002 1102 18-19 0.471549343574452 1102 20-21 0.43433914305276744 1102 22-23 0.33605523289509165 1102 24-25 0.4165494859156951 1102 26-27 0.4018887777542517 1102 28-29 0.4961586725178151 1102 30-31 0.5289908572332607 1102 32-33 0.29076151557884344 1102 34-35 0.2501747126636218 1102 36-37 0.26348886567436836 1102 38-39 0.3944618868007055 1102 40-41 0.28899943334796063 1102 42-43 0.3387590553254114 1102 44-45 0.2726782100302998 1102 46-47 0.2201107397481934 1102 48-49 0.2438446783663082 1102 50-51 0.36656321352037224 1102 52-53 0.24734371914071573 1102 54-55 -0.012143238723432148 1102 56-57 -3.296722235132627 1102 58-59 -10.059442937505015 1102 60-61 -8.255044245359617 1102 62-63 -2.419584325777162 1102 64-65 -0.695264592798754 1102 66-67 -1.8157472146284803 1102 68-69 -4.894982431956304 1102 70-71 -4.709343541811839 1102 72-73 -3.651497441799478 1102 74-75 -4.236528996779665 1102 76-77 -3.9743791992641704 1102 78-79 1.8054139497522677 1102 80-81 2.1480119132338764 1102 82-83 0.7905532342319077 1102 84-85 0.5296301368077287 1102 86-87 0.47163652681619794 1102 88-89 0.3677297857516528 1102 90-91 1.4858991299741753 1102 92-93 1.0075097001688214 1102 94-95 -8.688835486651275 1102 96-97 -9.89830988888301 1102 98-99 -8.888046656890879 1102 100-101 -9.787878958353414 1103 1 0.20596874852227387 1103 2 0.1748745170470727 1103 3 0.22972660648797572 1103 4 0.16953585619583578 1103 5 0.24449571782316326 1103 6 0.30806710271825466 1103 7 0.38797195363655135 1103 8 0.3994662699450231 1103 9 0.32227090576616035 1103 10-11 0.3457162405855101 1103 12-13 0.3871543926643426 1103 14-15 0.3755487320999791 1103 16-17 0.4179237421460087 1103 18-19 0.41598637584973375 1103 20-21 0.3374049914159585 1103 22-23 0.40121533574904333 1103 24-25 0.4050943086503054 1103 26-27 0.2988422751525732 1103 28-29 0.22689005146428798 1103 30-31 0.3302392956239757 1103 32-33 0.1698023539356015 1103 34-35 0.2778232019961635 1103 36-37 0.2731038304396094 1103 38-39 0.30828361469863097 1103 40-41 0.34114566062124396 1103 42-43 0.3111808535715639 1103 44-45 0.35018660936563606 1103 46-47 0.24584369546519724 1103 48-49 0.12845042508844529 1103 50-51 -0.0490389915325693 1103 52-53 0.2916526084878086 1103 54-55 0.31857431315838625 1103 56-57 0.600958941865926 1103 58-59 -8.619090165421131 1103 60-61 -7.5293211141359215 1103 62-63 -2.056980592469703 1103 64-65 -0.24257110486104594 1103 66-67 -1.8832883016298254 1103 68-69 -3.3885281037211943 1103 70-71 -3.78748707644621 1103 72-73 -2.988442570184983 1103 74-75 -3.8457973752531096 1103 76-77 -0.5698013492514349 1103 78-79 3.484487415130026 1103 80-81 2.915970965716081 1103 82-83 1.3381692784785173 1103 84-85 0.8627996275108494 1103 86-87 0.7337552818952169 1103 88-89 0.5902849423977408 1103 90-91 2.059341462624243 1103 92-93 1.4205908310771598 1103 94-95 -7.206052850533526 1103 96-97 -8.908762785895636 1103 98-99 -8.128422413437509 1103 100-101 -9.426498313818136 1104 1 0.12968311181306902 1104 2 0.18846362800340444 1104 3 0.15593798898570554 1104 4 0.06754433227276024 1104 5 0.17823912171176914 1104 6 0.08278501935048865 1104 7 0.2130969108429852 1104 8 0.20446455820246712 1104 9 0.2735488101210706 1104 10-11 0.12261172984874946 1104 12-13 0.18322747816883833 1104 14-15 0.24821255766750738 1104 16-17 0.20020012364029327 1104 18-19 0.2851325114433507 1104 20-21 0.29450175799330225 1104 22-23 0.19778376413327692 1104 24-25 0.31873294208948977 1104 26-27 0.32997385503193044 1104 28-29 0.29734690964937016 1104 30-31 0.2685290877358 1104 32-33 0.36624358214039887 1104 34-35 0.20285306895244304 1104 36-37 0.06129546296980237 1104 38-39 0.04442239570182238 1104 40-41 0.03337907147486874 1104 42-43 0.038521277847607394 1104 44-45 0.15813800768221853 1104 46-47 0.11876233457084595 1104 48-49 -0.10026470553521705 1104 50-51 0.08422648202985528 1104 52-53 -0.0027888912347648898 1104 54-55 -0.019087446512962458 1104 56-57 0.4374740701285731 1104 58-59 -8.200497749030902 1104 60-61 -7.477687462457826 1104 62-63 -2.1798805204584646 1104 64-65 -0.3383736760164133 1104 66-67 -1.6107633042859817 1104 68-69 -3.4811343202979437 1104 70-71 -3.889094933934789 1104 72-73 -2.9259827961350027 1104 74-75 -3.5385224693989485 1104 76-77 -0.09654951060382899 1104 78-79 3.6903062773524553 1104 80-81 3.0578952004491065 1104 82-83 1.5107058448410058 1104 84-85 1.0919101706644696 1104 86-87 1.1783475330137492 1104 88-89 1.1616028070874442 1104 90-91 2.5420091838817207 1104 92-93 2.3436025535623735 1104 94-95 -6.101702191217514 1104 96-97 -7.7717517244972 1104 98-99 -5.5511907143264345 1104 100-101 -5.091855595807063 1105 1 0.19331023906732625 1105 2 0.24501865868592887 1105 3 0.2202095504998418 1105 4 0.17390877054511122 1105 5 0.215346663318158 1105 6 0.19139209344869812 1105 7 0.18859023320644752 1105 8 0.27325659252566936 1105 9 0.2781223147390932 1105 10-11 0.2996203470000509 1105 12-13 0.2516343704173991 1105 14-15 0.4094449593895604 1105 16-17 0.4795153253866431 1105 18-19 0.4157778107774277 1105 20-21 0.32997497658466557 1105 22-23 0.49300528754733364 1105 24-25 0.44225063975893875 1105 26-27 0.4114268598763502 1105 28-29 0.38794260986251317 1105 30-31 0.5291413163877934 1105 32-33 0.44130240028339784 1105 34-35 0.49886023388344114 1105 36-37 0.539607121132974 1105 38-39 0.5417603944539096 1105 40-41 0.45965001731386224 1105 42-43 0.40715763332683963 1105 44-45 0.3972212775154276 1105 46-47 0.2825411834147715 1105 48-49 0.35635550480665046 1105 50-51 0.15719664065133543 1105 52-53 0.25288476458717923 1105 54-55 0.1781643669218269 1105 56-57 0.7597773048618492 1105 58-59 -8.183136791301738 1105 60-61 -7.619551462671332 1105 62-63 -2.1056443855731537 1105 64-65 -0.20650139777910326 1105 66-67 -1.5483395159420255 1105 68-69 -3.373418491188751 1105 70-71 -3.8082810141547743 1105 72-73 -2.8019775164215446 1105 74-75 -3.604097106435905 1105 76-77 -0.2759663010497313 1105 78-79 3.688947926809668 1105 80-81 3.1474035760238266 1105 82-83 1.6170659692460347 1105 84-85 1.2910370209109239 1105 86-87 1.1746507212723003 1105 88-89 0.9917071244518745 1105 90-91 2.5586902761207178 1105 92-93 2.3500888844698196 1105 94-95 -6.190292978453437 1105 96-97 -7.449294858568976 1105 98-99 -5.163822626637359 1105 100-101 -4.693397878148875 1106 1 0.0939263082039723 1106 2 0.12576311121152628 1106 3 0.11883801232352198 1106 4 0.08618041853005565 1106 5 0.12655142074093106 1106 6 0.1439159090842992 1106 7 0.15695073347740163 1106 8 0.15258298082035537 1106 9 0.02193934077942572 1106 10-11 0.17047088249532294 1106 12-13 0.20108675340704707 1106 14-15 0.011662790455417849 1106 16-17 0.16391180580647813 1106 18-19 0.12851953234840607 1106 20-21 0.1112909330605163 1106 22-23 0.23737581320262535 1106 24-25 0.21675213358399503 1106 26-27 0.21628259345496303 1106 28-29 0.3372746668489057 1106 30-31 0.2545297465523575 1106 32-33 0.3165729109647799 1106 34-35 0.14321444868051714 1106 36-37 0.14916483839716932 1106 38-39 0.1764600072715794 1106 40-41 0.21336051473264916 1106 42-43 0.2850601639163841 1106 44-45 0.41106857223035576 1106 46-47 0.45043918618138434 1106 48-49 0.318430531189243 1106 50-51 0.23837480157087754 1106 52-53 0.328695048806118 1106 54-55 0.212884657985974 1106 56-57 0.8171857323168581 1106 58-59 -8.01113923703917 1106 60-61 -7.406794588936982 1106 62-63 -1.9898453139551187 1106 64-65 -0.25406314730136614 1106 66-67 0.6605379583171214 1106 68-69 -2.265822533429418 1106 70-71 -2.4346687740589417 1106 72-73 -1.1292847452512973 1106 74-75 -0.9342257572159767 1106 76-77 2.321962576478011 1106 78-79 4.584964868710927 1106 80-81 3.5649232203579864 1106 82-83 1.958228960176129 1106 84-85 1.4267585134524943 1106 86-87 1.2275085975450821 1106 88-89 1.170643032583417 1106 90-91 2.370913080661378 1106 92-93 1.8917778337296802 1106 94-95 -6.164587333292147 1106 96-97 -7.638477640007041 1106 98-99 -5.4912274532253065 1106 100-101 -5.065258260767738 1107 1 0.06191337706074407 1107 2 0.14244195012292593 1107 3 0.08747940276940369 1107 4 0.07972235035446573 1107 5 -0.03816982543013836 1107 6 0.04385407640097583 1107 7 0.053144141239044984 1107 8 0.1297384903451544 1107 9 0.13813139139740827 1107 10-11 0.10290356033986825 1107 12-13 0.07667153225788326 1107 14-15 0.14754429876401076 1107 16-17 0.1664184436309455 1107 18-19 0.13453739879676618 1107 20-21 0.20161134511021572 1107 22-23 0.18763524730851344 1107 24-25 0.24562014527282372 1107 26-27 0.20847488505294365 1107 28-29 0.17559872926707243 1107 30-31 0.07145699277737094 1107 32-33 0.09947358588276245 1107 34-35 0.09445041725045655 1107 36-37 0.07523030491965699 1107 38-39 0.1668468519386508 1107 40-41 0.1519666194432432 1107 42-43 0.09421112918482066 1107 44-45 0.01961576081155414 1107 46-47 0.12721785997362645 1107 48-49 0.05976346158430346 1107 50-51 0.03880399825492731 1107 52-53 0.12023060466518842 1107 54-55 0.09066680249915748 1107 56-57 0.6531562705548168 1107 58-59 -8.258908704003595 1107 60-61 -7.479031985987632 1107 62-63 -1.901919803743045 1107 64-65 -0.26636590473890465 1107 66-67 0.6975700917382639 1107 68-69 -2.3918533318909745 1107 70-71 -2.7727779010240816 1107 72-73 -1.2971651021626833 1107 74-75 -0.9638852729244753 1107 76-77 2.0224291163619377 1107 78-79 4.212170770893927 1107 80-81 3.1408150638375645 1107 82-83 1.5935678828824464 1107 84-85 1.2323048920973179 1107 86-87 1.0505248841390298 1107 88-89 0.9537312390919652 1107 90-91 2.2724322442258966 1107 92-93 1.9312964326876383 1107 94-95 -6.720445811481717 1107 96-97 -8.367411410091245 1107 98-99 -6.364987482821478 1107 100-101 -5.642920317640758 1108 1 -0.08043295322425337 1108 2 -0.05496650400084491 1108 3 -0.03067196046231402 1108 4 -0.027497283078680823 1108 5 -0.03890553609533498 1108 6 0.024674805360440644 1108 7 -0.10904103337643534 1108 8 -0.0018324313536766113 1108 9 -0.05398301272913386 1108 10-11 -0.01909343250002138 1108 12-13 -0.07929880930610267 1108 14-15 -0.045668240582507735 1108 16-17 -0.15903550550289935 1108 18-19 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0.25219013531587464 2311 66-67 -1.8776516803389782 2311 68-69 -4.85291553018231 2311 70-71 -4.597367052398457 2311 72-73 -6.7695633671961915 2311 74-75 -7.30140136030915 2311 76-77 -5.272997553142016 2311 78-79 -0.44944439231618816 2311 80-81 1.592488121210561 2311 82-83 0.5607430320749422 2311 84-85 0.3427906096038633 2311 86-87 0.3819278674579749 2311 88-89 0.40976662745807246 2311 90-91 1.8506477708648923 2311 92-93 2.3069630559236423 2311 94-95 -1.013201903653293 2311 96-97 -5.96512427513856 2311 98-99 -5.457400598691084 2311 100-101 -6.023295883277491 2312 1 -0.27139610708841744 2312 2 -0.245581670990898 2312 3 -0.22941432716985233 2312 4 -0.1903659527530337 2312 5 -0.23637588578841928 2312 6 -0.14954807685969485 2312 7 -0.1939192776497265 2312 8 -0.23139226962112502 2312 9 -0.2845603257308298 2312 10-11 -0.28866938053609914 2312 12-13 -0.41276854000314245 2312 14-15 -0.3887270035427406 2312 16-17 -0.3208053580151926 2312 18-19 -0.34764395380670265 2312 20-21 -0.4640875757743217 2312 22-23 -0.5193237141577143 2312 24-25 -0.436852918927471 2312 26-27 -0.38408465047718465 2312 28-29 -0.38721711585031215 2312 30-31 -0.33619906631329144 2312 32-33 -0.3573432574933264 2312 34-35 -0.3600644788197087 2312 36-37 -0.34881294948390007 2312 38-39 -0.274093198727833 2312 40-41 -0.3875415081683329 2312 42-43 -0.3495949821585995 2312 44-45 -0.332306358174435 2312 46-47 -0.27754152530663134 2312 48-49 -0.35395515030901237 2312 50-51 -0.21656214333888357 2312 52-53 -0.3399832944589747 2312 54-55 -0.3111323779506918 2312 56-57 0.3396483231148153 2312 58-59 3.192830618000176 2312 60-61 3.160137623498013 2312 62-63 0.6563954097216858 2312 64-65 -0.0637350691667038 2312 66-67 -2.1131927444588285 2312 68-69 -5.122870107787904 2312 70-71 -4.8145717925666105 2312 72-73 -7.185422405198732 2312 74-75 -7.56344173822286 2312 76-77 -5.1311183946700645 2312 78-79 -0.44769301619418655 2312 80-81 1.1828607747070912 2312 82-83 0.30196080159871386 2312 84-85 -0.008594063819469255 2312 86-87 0.07308906825593198 2312 88-89 0.1719293791161398 2312 90-91 1.9121328487826723 2312 92-93 3.2763985367568615 2312 94-95 5.120682297016437 2312 96-97 0.13553286188084357 2312 98-99 -0.9713656164464517 2312 100-101 -0.5209466212867664 2313 1 -0.34993139956319297 2313 2 -0.3032913386753755 2313 3 -0.2512719126573586 2313 4 -0.24050222292411405 2313 5 -0.24867965767927558 2313 6 -0.19061282112350852 2313 7 -0.24043295433890677 2313 8 -0.3244601104230753 2313 9 -0.19788987274029068 2313 10-11 -0.37735970610372505 2313 12-13 -0.3226160748626299 2313 14-15 -0.46806984204852853 2313 16-17 -0.4857783945157266 2313 18-19 -0.5145086527096083 2313 20-21 -0.40496754930754264 2313 22-23 -0.3316871393937504 2313 24-25 -0.39292875037233443 2313 26-27 -0.5577422393386442 2313 28-29 -0.4791994344675956 2313 30-31 -0.5274146074858166 2313 32-33 -0.6079329961807503 2313 34-35 -0.5116172464912481 2313 36-37 -0.7079465186189893 2313 38-39 -0.65833051688589 2313 40-41 -0.6740754098251287 2313 42-43 -0.6695437074608108 2313 44-45 -0.7610700015926923 2313 46-47 -0.8502806963266352 2313 48-49 -0.8443684875411321 2313 50-51 -0.9589332161993767 2313 52-53 -0.6395320752439417 2313 54-55 -0.7066097359213686 2313 56-57 0.03392488220863754 2313 58-59 3.0377027264764784 2313 60-61 3.07408062057954 2313 62-63 0.2800534671875816 2313 64-65 -0.4339916675858184 2313 66-67 -2.163548508949482 2313 68-69 -4.534379595153393 2313 70-71 -3.457166577186637 2313 72-73 -2.650804533375169 2313 74-75 -3.551625596112004 2313 76-77 0.38908272098338514 2313 78-79 2.7733185976982497 2313 80-81 2.210286599310326 2313 82-83 0.6335468964180109 2313 84-85 0.3019682333919569 2313 86-87 0.17393827322163702 2313 88-89 0.323735333766475 2313 90-91 2.0561946464617726 2313 92-93 3.370812959276865 2313 94-95 5.73395561149313 2313 96-97 1.341308083577605 2313 98-99 2.889884123478083 2313 100-101 3.9203231374194374 2314 1 -0.3544305031465882 2314 2 -0.3253946270491923 2314 3 -0.41127471159527573 2314 4 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64-65 -0.05996618968608303 2314 66-67 -2.1237005215371383 2314 68-69 -4.99164813433474 2314 70-71 -3.8118546090794005 2314 72-73 -2.683732335906317 2314 74-75 -3.9009145145872637 2314 76-77 0.5997175503616425 2314 78-79 3.4050988356836207 2314 80-81 2.456652329719649 2314 82-83 1.0809527726746957 2314 84-85 0.7390953883850351 2314 86-87 0.6425020398356054 2314 88-89 0.8341990118734834 2314 90-91 2.351846458584955 2314 92-93 3.9211021198224323 2314 94-95 6.53231463958085 2314 96-97 3.839483100409055 2314 98-99 6.17605566811358 2314 100-101 6.423723846988022 2315 1 -0.44659596576794414 2315 2 -0.375910882694388 2315 3 -0.48700357459642873 2315 4 -0.3478799863248412 2315 5 -0.46086461176516735 2315 6 -0.46448563036354074 2315 7 -0.46768485831102424 2315 8 -0.6113840805578761 2315 9 -0.7161446319693852 2315 10-11 -0.6545629005510207 2315 12-13 -0.6363208409166674 2315 14-15 -0.7099878473701082 2315 16-17 -0.5976323856115116 2315 18-19 -0.5982281718596454 2315 20-21 -0.7095181501830083 2315 22-23 -0.7408101716130915 2315 24-25 -0.4692783594807324 2315 26-27 -0.5571559486858249 2315 28-29 -0.5378302293584198 2315 30-31 -0.5622347188165335 2315 32-33 -0.617949067519163 2315 34-35 -0.5568502575252197 2315 36-37 -0.5267792476799684 2315 38-39 -0.6616647820265129 2315 40-41 -0.663915874444811 2315 42-43 -0.581870255741201 2315 44-45 -0.5988138530669715 2315 46-47 -0.4190664454716426 2315 48-49 -0.49695845992348353 2315 50-51 -0.45165609733505363 2315 52-53 -0.4840943252687211 2315 54-55 -0.39032846038626445 2315 56-57 0.23778602360088996 2315 58-59 3.322955941211621 2315 60-61 3.3993322085474453 2315 62-63 0.67198629104983 2315 64-65 -0.013637957902066233 2315 66-67 -1.8257152320232102 2315 68-69 -4.83487940536388 2315 70-71 -3.378755508063989 2315 72-73 -2.8196998088317393 2315 74-75 -0.43477939293064694 2315 76-77 2.06349054031125 2315 78-79 3.8122187628057773 2315 80-81 2.661789208682375 2315 82-83 0.8629819407240475 2315 84-85 0.6689323771981819 2315 86-87 0.6448039003778092 2315 88-89 0.7173400349368109 2315 90-91 2.3643136641187397 2315 92-93 3.8345930801811967 2315 94-95 6.604137902916328 2315 96-97 4.012736862024994 2315 98-99 6.481383469966417 2315 100-101 6.646079958155294 2316 1 -0.4916251394164419 2316 2 -0.4179663420006854 2316 3 -0.5192171990075529 2316 4 -0.38072581130720806 2316 5 -0.5150908372806384 2316 6 -0.5657624600768258 2316 7 -0.3990402233756285 2316 8 -0.4538749833962328 2316 9 -0.49790463165075494 2316 10-11 -0.5769706903772871 2316 12-13 -0.449398304405598 2316 14-15 -0.633356565770832 2316 16-17 -0.7062126526800441 2316 18-19 -0.7022504665250437 2316 20-21 -0.6501294964633573 2316 22-23 -0.903067562154547 2316 24-25 -0.7353181633138917 2316 26-27 -0.6981480364929027 2316 28-29 -0.6974782084212094 2316 30-31 -0.7438527539853155 2316 32-33 -0.6154543464935074 2316 34-35 -0.5734402270258911 2316 36-37 -0.38940167430727257 2316 38-39 -0.630363702569376 2316 40-41 -0.6845315512628716 2316 42-43 -0.6794118156417994 2316 44-45 -0.6277341325206294 2316 46-47 -0.7711287961624649 2316 48-49 -0.7649747052332359 2316 50-51 -0.7679159719612727 2316 52-53 -0.6311868679723531 2316 54-55 -0.5711999666158931 2316 56-57 0.2695651911618313 2316 58-59 3.1306636827809946 2316 60-61 3.1326264397081864 2316 62-63 0.5921114059326378 2316 64-65 0.09648361488332924 2316 66-67 -1.906662839487126 2316 68-69 -3.2226858006375103 2316 70-71 -3.575492361003217 2316 72-73 -0.8886460153884315 2316 74-75 5.330724751269017 2316 76-77 5.545415614586219 2316 78-79 4.671568619107781 2316 80-81 3.036077066511833 2316 82-83 1.3443120639316462 2316 84-85 0.9713079179156914 2316 86-87 0.9110701431755963 2316 88-89 0.8489891505847105 2316 90-91 2.4048152805118512 2316 92-93 3.954339282066993 2316 94-95 6.525734169529358 2316 96-97 3.366970139147636 2316 98-99 5.768430817208827 2316 100-101 5.913354517414007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 42.0 5 137.0 6 321.0 7 769.0 8 1651.0 9 2881.0 10 4644.0 11 7460.0 12 10775.0 13 14543.0 14 17953.0 15 23479.0 16 31331.0 17 41221.0 18 43696.0 19 42023.0 20 43667.0 21 45212.0 22 45590.0 23 46031.0 24 48442.0 25 51198.0 26 64072.0 27 90058.0 28 127196.0 29 169339.0 30 205067.0 31 224844.0 32 232836.0 33 231564.0 34 238237.0 35 254302.0 36 254892.0 37 193444.0 38 76603.0 39 4900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.607473624506824 19.878437586622045 14.512360933228916 23.001727855642212 2 15.17974539183582 21.84002701647839 39.86715975430506 23.11306783738073 3 18.665966426529966 26.96100970199316 30.70805556391646 23.664968307560414 4 12.473719444583791 17.56030645800214 38.27853496606924 31.68743913134483 5 12.696166188494724 38.96845726970802 34.67308029861195 13.662296243185315 6 30.02132889572367 37.79570273781373 18.10901089624608 14.073957470216525 7 26.63593322135519 34.00939993668795 22.03155569471137 17.32311114724549 8 25.303664524780455 35.28414156584302 20.706133973235243 18.706059936141276 9 26.598534135747265 16.45878056838999 20.738156235458554 36.2045290604042 10-11 24.14977624552621 26.922480794479743 28.442125693448588 20.485617266545464 12-13 25.821093291863363 24.51309400455639 28.44638109356864 21.219431610011608 14-15 22.781676472603 25.60790347166819 27.1904383644487 24.41998169128011 16-17 21.34717019253789 28.798205396817927 27.81290694440024 22.04171746624394 18-19 21.191586380238945 27.76835300599478 30.04610901655721 20.99395159720907 20-21 22.425993229416232 26.89803819135116 29.54140279437941 21.134565784853198 22-23 21.841948212126503 26.805145920437855 29.278329115413026 22.07457675202261 24-25 21.779937307220397 26.921361057096927 29.72336953710753 21.57533209857515 26-27 21.930790539720036 26.85607361916495 29.764053468620556 21.44908237249446 28-29 21.500631138725797 26.906403087035564 30.066369465513638 21.526596308725008 30-31 21.70367738312674 27.324845370134266 29.646464419302017 21.32501282743698 32-33 21.458190540873908 27.39461440759625 29.433387988030173 21.713807063499672 34-35 21.900133425062705 26.949094870184314 29.407053347363753 21.743718357389227 36-37 22.176505694818403 26.803503443485273 29.49741580614884 21.522575055547485 38-39 22.138687713554024 26.8523366116949 29.637818608406846 21.37115706634423 40-41 21.935588424677803 26.865899048444508 29.841208885293753 21.35730364158394 42-43 21.61123623675619 27.255741812696606 29.2833832681618 21.849638682385404 44-45 21.712329490567274 27.5244763575433 28.877833700156202 21.885360451733227 46-47 21.820219540408797 27.2752614398719 28.426741343902833 22.477777675816462 48-49 21.92961650806138 27.344073526743607 28.532037760569956 22.194272204625058 50-51 22.308271440736398 27.570730501430006 28.329298910645285 21.791699147188307 52-53 22.018754678933224 27.627295111407292 28.53323178515637 21.820718424503113 54-55 21.757093340445554 27.39853533728555 28.602997413051117 22.241373909217774 56-57 21.799205413202618 28.028182886869445 28.015805875843174 22.156805824084763 58-59 20.80422470176952 27.232685527846485 29.813903900540662 22.149185869843333 60-61 21.2817663104691 27.343106523632294 29.237969524612133 22.137157641286475 62-63 22.37440829129125 27.286671754848363 28.44536905116944 21.893550902690947 64-65 22.078415206631814 27.263027352375758 28.582529471567746 22.07602796942468 66-67 21.515710998748155 27.367559742357805 28.985131723419283 22.131597535474757 68-69 21.055626251188784 27.074999451194167 29.97115405723301 21.898220240384035 70-71 21.879755889559107 26.95339507014157 28.8027557476015 22.36409329269782 72-73 20.982925296698564 27.135808003031027 29.01572785405718 22.865538846213234 74-75 21.268440975129916 27.245307553787036 28.7981441977542 22.68810727332885 76-77 20.181998689057462 27.398228589768024 29.495004862892905 22.924767858281605 78-79 21.326123227681908 27.235079409034803 28.567718588797153 22.871078774486133 80-81 21.08946743166061 28.009368897302938 28.065783018484275 22.835380652552175 82-83 21.5951489713412 27.58868593813707 27.833199011153496 22.982966079368232 84-85 21.529618757776802 27.27599041864873 27.686832284663076 23.507558538911397 86-87 21.49381064566524 27.272634489301073 28.110077250379835 23.123477614653858 88-89 21.807466570441527 26.921664443536088 28.09221145474734 23.178657531275046 90-91 21.235578727819785 27.169011227059208 28.36939768600159 23.22601235911942 92-93 20.78597535045441 27.460484797358404 28.306234664130947 23.44730518805624 94-95 19.891148172912917 27.48088764431378 28.654601892118848 23.973362290654453 96-97 19.668124883970815 27.349098769668707 28.418360458378572 24.56441588798191 98-99 20.39745794855843 27.013918130263338 28.27136637614932 24.317257545028912 100-101 19.762779508677312 27.23868272602849 28.725128573861113 24.273409191433082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1570.0 1 1135.5 2 751.0 3 840.5 4 846.5 5 958.5 6 1286.0 7 1901.5 8 2664.5 9 3182.5 10 3358.0 11 3415.0 12 3588.0 13 3807.5 14 4019.0 15 4214.0 16 4574.5 17 5008.5 18 5384.0 19 5854.5 20 6487.5 21 7337.0 22 8500.0 23 9802.5 24 11467.0 25 13959.5 26 17262.0 27 21117.0 28 25302.0 29 30069.5 30 35555.5 31 42019.5 32 49364.0 33 56654.0 34 63047.5 35 70145.0 36 77890.5 37 84014.0 38 88474.0 39 91205.0 40 93159.5 41 94893.0 42 96749.0 43 99707.5 44 102325.5 45 104654.5 46 108497.5 47 114321.5 48 123445.0 49 122021.5 50 110497.0 51 102833.0 52 96833.0 53 91091.0 54 84938.0 55 78331.5 56 71320.0 57 63835.0 58 56369.0 59 48975.0 60 40526.5 61 31883.5 62 24469.5 63 18367.0 64 13452.0 65 9479.0 66 6461.0 67 4483.0 68 3070.0 69 2035.5 70 1353.0 71 898.5 72 643.5 73 459.5 74 318.5 75 201.0 76 114.5 77 62.0 78 39.5 79 30.5 80 23.5 81 11.0 82 5.0 83 4.0 84 3.0 85 1.5 86 1.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025463207222417312 2 0.012351039372830136 3 2.7677399154801423E-4 4 0.0 5 0.06967785237221258 6 0.0 7 0.0 8 6.919349788700356E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 6.573382299265338E-4 16-17 3.459674894350178E-5 18-19 4.3245936179377224E-4 20-21 0.0 22-23 0.0 24-25 1.729837447175089E-5 26-27 6.919349788700356E-5 28-29 0.0011935878385508113 30-31 0.005397092835186278 32-33 0.006158221311943316 34-35 0.0020239098131948543 36-37 0.08996884562757637 38-39 0.08362034219644379 40-41 0.08976126513391536 42-43 0.09275388391752827 44-45 0.09726875965465526 46-47 0.09131811883637295 48-49 0.08140615026405969 50-51 0.07526522732658812 52-53 0.063398542438967 54-55 0.05260435676859446 56-57 0.07007571498506285 58-59 0.685171314451581 60-61 0.32403315060483767 62-63 0.0030099171580846547 64-65 0.0025774577962908826 66-67 1.0609093063524822 68-69 2.287917604382716 70-71 2.2715533421324396 72-73 1.746564975289272 74-75 1.2396188130201404 76-77 0.7180209275734359 78-79 0.5521987098872319 80-81 0.037969931965493205 82-83 2.2487886813276156E-4 84-85 0.0013319748343248185 86-87 0.0013319748343248185 88-89 5.1895123415252664E-5 90-91 0.3257110929285975 92-93 0.05495693569675257 94-95 0.9154299770450571 96-97 1.6029711687992678 98-99 0.6241426493152439 100-101 0.5443971430004723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2890445.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.189126324676735 #Duplication Level Percentage of deduplicated Percentage of total 1 65.85542508258253 27.12527422891776 2 15.58049132402667 12.834936506917288 3 6.782937771042525 8.381498421116753 4 3.5249446009366756 5.807575538218717 5 2.168446978987433 4.465821827293851 6 1.33080168664979 3.288873527070664 7 0.9263143771128012 2.6707855926684387 8 0.6493360034014508 2.1396466137010464 9 0.5000670710719661 1.853759318507488 >10 2.454478824244326 17.626527040649602 >50 0.14924385149407626 4.233126040777288 >100 0.07179834035537475 5.285504702888981 >500 0.003445258998103702 0.9061640524611385 >1k 0.0017646448526327137 1.476734863501628 >5k 3.361228290728978E-4 1.0599776214019911 >10k+ 1.680614145364489E-4 0.8437941039072373 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13978 0.4835933567322679 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10401 0.35984078576136197 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9572 0.331160080887199 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 8049 0.2784692322462458 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7823 0.27065036698501443 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5181 0.17924575627628273 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3987 0.1379372380377416 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3937 0.13620740059056652 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3394 0.11742136591424504 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3138 0.10856459818470857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 6.919349788700356E-5 10-11 0.0 0.0 0.0 0.0 6.919349788700356E-5 12-13 0.0 1.729837447175089E-5 0.0 3.459674894350178E-5 6.919349788700356E-5 14-15 0.0 3.459674894350178E-5 0.0 6.919349788700356E-5 1.3838699577400711E-4 16-17 0.0 3.459674894350178E-5 0.0 1.0379024683050534E-4 1.7298374471750889E-4 18-19 0.0 3.459674894350178E-5 0.0 1.9028211918925979E-4 1.7298374471750889E-4 20-21 0.0 6.919349788700356E-5 0.0 3.286691149632669E-4 1.7298374471750889E-4 22-23 0.0 6.919349788700356E-5 0.0 8.130236001722918E-4 1.9028211918925979E-4 24-25 0.0 6.919349788700356E-5 0.0 0.0015568537024575801 2.4217724260451246E-4 26-27 0.0 6.919349788700356E-5 0.0 0.002369877302629872 2.7677399154801423E-4 28-29 0.0 6.919349788700356E-5 0.0 0.005293302588355772 2.7677399154801423E-4 30-31 0.0 6.919349788700356E-5 0.0 0.013665715832683204 2.7677399154801423E-4 32-33 0.0 6.919349788700356E-5 0.0 0.03151763828753012 2.7677399154801423E-4 34-35 0.0 6.919349788700356E-5 0.0 0.0564964910247384 3.1137074049151603E-4 36-37 0.0 1.0379024683050534E-4 0.0 0.09770121901644901 3.1137074049151603E-4 38-39 0.0 1.3838699577400711E-4 0.0 0.15597944261177776 3.1137074049151603E-4 40-41 0.0 1.3838699577400711E-4 0.0 0.22453290064332654 3.4596748943501777E-4 42-43 0.0 1.3838699577400711E-4 0.0 0.2919446659597398 3.4596748943501777E-4 44-45 0.0 1.3838699577400711E-4 0.0 0.3712231161637741 3.632658639067687E-4 46-47 0.0 1.3838699577400711E-4 0.0 0.46354454071950857 3.8056423837851957E-4 48-49 0.0 1.3838699577400711E-4 0.0 0.5607614052507486 3.8056423837851957E-4 50-51 0.0 1.3838699577400711E-4 0.0 0.6605557275782794 3.8056423837851957E-4 52-53 1.729837447175089E-5 1.7298374471750889E-4 0.0 0.7547972717003784 3.8056423837851957E-4 54-55 6.919349788700356E-5 3.286691149632669E-4 0.0 0.8580858656712029 3.8056423837851957E-4 56-57 6.919349788700356E-5 3.8056423837851957E-4 0.0 0.9612360726462534 3.8056423837851957E-4 58-59 6.919349788700356E-5 4.497577362655231E-4 0.0 1.0424000456677085 3.8056423837851957E-4 60-61 6.919349788700356E-5 4.497577362655231E-4 0.0 1.1180458372326751 3.8056423837851957E-4 62-63 8.649187235875444E-5 4.497577362655231E-4 0.0 1.1826552658846647 3.8056423837851957E-4 64-65 1.0379024683050534E-4 4.497577362655231E-4 0.0 1.2505686840607588 3.9786261285027044E-4 66-67 1.0379024683050534E-4 4.497577362655231E-4 0.0 1.3116492443205112 4.1516098732202137E-4 68-69 1.0379024683050534E-4 4.497577362655231E-4 0.0 1.3830569341398988 4.1516098732202137E-4 70-71 1.0379024683050534E-4 4.497577362655231E-4 0.0 1.4929016120355172 4.1516098732202137E-4 72-73 1.0379024683050534E-4 4.497577362655231E-4 0.0 1.6398167064240972 4.1516098732202137E-4 74-75 1.0379024683050534E-4 4.6705611073727404E-4 0.0 1.834215838737634 4.1516098732202137E-4 76-77 1.3838699577400711E-4 4.843544852090249E-4 0.0 2.081824770926276 4.1516098732202137E-4 78-79 1.3838699577400711E-4 4.843544852090249E-4 0.0 2.390427771502312 4.1516098732202137E-4 80-81 1.3838699577400711E-4 5.535479830960285E-4 0.0 2.7614952022958406 4.3245936179377224E-4 82-83 1.3838699577400711E-4 5.535479830960285E-4 0.0 3.13524388113249 4.497577362655231E-4 84-85 1.3838699577400711E-4 5.535479830960285E-4 0.0 3.4790490737585387 4.497577362655231E-4 86-87 1.3838699577400711E-4 5.881447320395302E-4 0.0 3.800625855188388 4.497577362655231E-4 88-89 1.3838699577400711E-4 5.881447320395302E-4 0.0 4.0968778163915935 4.497577362655231E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 14110 0.0 25.295462 1 TACATGG 14060 0.0 24.773708 2 ACATGGG 14195 0.0 23.63832 3 GTATCAA 24235 0.0 21.779434 1 CATGGGA 9280 0.0 20.901442 4 GAGTACT 10620 0.0 20.605124 12-13 AGTACTT 11280 0.0 20.382023 12-13 ATCAACG 27225 0.0 19.29344 3 TCAACGC 27305 0.0 19.254198 4 TATCAAC 27815 0.0 18.9028 2 CAACGCA 27790 0.0 18.884201 5 CATGGGG 5430 0.0 18.860025 4 GTACTTT 11445 0.0 18.83138 14-15 AACGCAG 27965 0.0 18.769257 6 ATGGGAG 3630 0.0 18.591389 5 TACTTTT 12900 0.0 17.56651 14-15 GGTATCA 18550 0.0 17.51025 1 ATGGGAT 4055 0.0 17.45753 5 ACGCAGA 29605 0.0 17.394995 7 CGCAGAG 29635 0.0 17.234167 8 >>END_MODULE