FastQCFastQC Report
Fri 27 May 2016
SRR939665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112196
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72840.6549205355890508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49690.4467737700908833No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35430.3185589590324007No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33870.30453265431632554No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18860.1695744275289607No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16900.15195163442414825No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16200.1456577797438581No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15900.14296041345230517No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12170.10942315922733044No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG50750.028.7078251
TACATGG53550.027.1181412
ACATGGG53550.026.2319283
CATGGGA31950.025.6987694
GTATCAA107250.024.8235281
GAGTACT50650.023.00326712-13
ATGGGAT13050.022.1848435
GGTATCA79600.021.7013261
TATCAAC123300.021.5537532
CATGGGG21900.021.4549794
TCAACGC123600.021.3881764
CAACGCA123950.021.3277825
ATCAACG125150.021.235143
AGAGTAC77450.020.98730510-11
ATGGGAG12700.020.927695
GTACTTT55500.020.9075614-15
AACGCAG126000.020.8640236
AGTACTT52750.020.46804812-13
ACGCAGA135850.019.0718087
CGCAGAG135450.018.8854458
Produced by FastQC (version 0.11.5)