##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939665_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112196 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.285631309589316 31.0 30.0 33.0 26.0 34.0 2 30.397840848195823 31.0 30.0 34.0 26.0 34.0 3 30.422306859582303 31.0 30.0 34.0 26.0 34.0 4 34.03299238623408 35.0 35.0 37.0 28.0 37.0 5 34.037698391290746 35.0 35.0 37.0 28.0 37.0 6 34.16920129185863 36.0 35.0 37.0 30.0 37.0 7 33.9761130232441 36.0 35.0 37.0 29.0 37.0 8 33.99234937007506 36.0 35.0 37.0 29.0 37.0 9 35.26093782031225 38.0 35.0 39.0 28.0 39.0 10-11 35.156768681059816 37.0 34.5 39.0 28.5 39.0 12-13 35.010996263248565 37.0 34.0 39.0 27.0 39.0 14-15 35.65001582454891 38.0 34.0 40.0 27.0 41.0 16-17 35.39754099097641 38.0 34.0 40.0 27.0 41.0 18-19 35.414570363497084 38.0 34.0 40.0 27.0 41.0 20-21 35.525805703311285 38.0 34.0 40.0 27.0 41.0 22-23 35.35325068603016 38.0 34.0 40.0 27.0 41.0 24-25 35.20826814698129 38.0 34.0 40.0 26.0 41.0 26-27 35.302501987059834 38.0 34.0 40.0 26.0 41.0 28-29 35.168415908706734 38.0 34.0 40.0 25.0 41.0 30-31 34.910289643192385 38.0 34.0 40.0 25.0 41.0 32-33 34.616433614219076 38.0 33.0 40.0 24.0 41.0 34-35 34.34860716995925 38.0 33.0 40.0 24.0 41.0 36-37 34.01430413344411 38.0 33.0 40.0 23.0 41.0 38-39 33.818517149854884 38.0 33.0 40.0 22.0 41.0 40-41 33.4950818021284 37.5 32.5 40.0 20.0 41.0 42-43 33.11945376534352 37.0 31.5 40.0 17.5 41.0 44-45 32.79848605821276 37.0 31.0 40.0 16.0 41.0 46-47 32.23897226747803 37.0 30.5 40.0 11.0 41.0 48-49 31.80426696373661 36.5 30.0 39.0 8.5 41.0 50-51 31.131085258353743 35.5 29.5 38.5 8.0 40.0 52-53 31.14512999507281 36.0 30.0 39.0 7.0 40.0 54-55 31.172667857104322 36.0 29.0 39.0 2.0 40.0 56-57 30.99707155932947 36.0 29.0 39.0 2.0 40.0 58-59 30.59359726163374 35.0 28.5 39.0 2.0 40.0 60-61 30.151428345363588 35.0 28.0 38.5 2.0 40.0 62-63 29.730762383608642 34.5 28.0 38.0 2.0 40.0 64-65 29.1127535074753 34.0 26.5 38.0 2.0 40.0 66-67 28.56548081453269 34.0 26.0 37.0 2.0 39.0 68-69 28.041686896913852 33.5 26.0 36.5 2.0 39.0 70-71 27.46608736229945 33.0 24.5 36.0 2.0 39.0 72-73 26.73020133142 32.0 22.5 35.5 2.0 38.0 74-75 26.294192300637658 32.0 22.0 35.0 2.0 37.5 76-77 25.93703852558362 32.0 20.5 35.0 2.0 37.0 78-79 25.377290513542576 31.0 19.5 35.0 2.0 36.0 80-81 24.710415250549364 31.0 17.5 34.0 2.0 36.0 82-83 24.062338832364077 30.5 10.5 34.0 2.0 35.0 84-85 23.404098288431175 30.0 7.0 34.0 2.0 35.0 86-87 22.751814877953166 29.5 2.0 34.0 2.0 35.0 88-89 21.976475819010318 29.0 2.0 34.0 2.0 35.0 90-91 20.779391402234857 28.0 2.0 33.5 2.0 35.0 92-93 19.183202870717032 25.0 2.0 32.5 2.0 34.0 94-95 18.90029455239904 25.0 2.0 32.0 2.0 34.0 96-97 18.25605648644663 25.0 2.0 32.0 2.0 34.0 98-99 17.280571050426364 22.0 2.0 32.0 2.0 34.0 100-101 15.647323852989942 9.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.28095321738709 1101 2 1.19875714267328 1101 3 1.1428497206758728 1101 4 1.0294225067957683 1101 5 0.8629810136999225 1101 6 0.8025369640209092 1101 7 1.0035315480454514 1101 8 0.8190414010690148 1101 9 1.2937084501287828 1101 10-11 0.9394484388836659 1101 12-13 0.791423535116401 1101 14-15 1.0299185820583432 1101 16-17 0.6852727243796508 1101 18-19 0.8543589669911427 1101 20-21 1.1336664742103224 1101 22-23 1.3821868654433231 1101 24-25 1.0592257395307811 1101 26-27 0.965058208553458 1101 28-29 1.325135559861458 1101 30-31 1.2455034968618364 1101 32-33 1.1603867652361757 1101 34-35 1.099730466762054 1101 36-37 1.0800745875256652 1101 38-39 1.1418736663770233 1101 40-41 1.035086599538701 1101 42-43 1.1092944379543468 1101 44-45 0.9819423599661903 1101 46-47 0.7134922350927084 1101 48-49 0.6390117270333917 1101 50-51 0.937070556688969 1101 52-53 0.7055496216802801 1101 54-55 0.8553071992873242 1101 56-57 0.7246663075940312 1101 58-59 0.9377359870982893 1101 60-61 0.4889251624998323 1101 62-63 0.7838656076313057 1101 64-65 0.8066359357471029 1101 66-67 0.6265092636640617 1101 68-69 0.4464861024462401 1101 70-71 0.6732817622645086 1101 72-73 0.6694249833195052 1101 74-75 0.3372640705241743 1101 76-77 0.22555442611347232 1101 78-79 0.272964598672349 1101 80-81 -0.17053523991472375 1101 82-83 0.031982813994694936 1101 84-85 -0.34073937202886384 1101 86-87 -1.3120032030820532 1101 88-89 -1.6127062334258468 1101 90-91 -4.402904412209384 1101 92-93 -9.572927680761588 1101 94-95 -8.379521515290579 1101 96-97 -5.365529672504266 1101 98-99 -4.236524204380972 1101 100-101 -3.399715807138259 1102 1 0.9892939601822377 1102 2 0.891607351905602 1102 3 1.001028542938517 1102 4 1.1175219594472736 1102 5 0.8873775844106575 1102 6 0.8410748664770438 1102 7 1.133389367950663 1102 8 0.9338866996635247 1102 9 1.003670845410653 1102 10-11 0.7841564517067923 1102 12-13 0.7934979125176724 1102 14-15 1.0240013450670418 1102 16-17 1.2753919011544568 1102 18-19 1.1761525733733436 1102 20-21 1.2441256433173535 1102 22-23 1.1355480420386712 1102 24-25 1.144663495972928 1102 26-27 1.1359288271143626 1102 28-29 1.2331410415036643 1102 30-31 1.2753188992975737 1102 32-33 1.4340259394006765 1102 34-35 1.413437019445368 1102 36-37 1.2054638901661576 1102 38-39 0.9925470437048816 1102 40-41 1.1789984690886683 1102 42-43 1.1976263709673773 1102 44-45 1.0959156532748366 1102 46-47 1.1846948519242844 1102 48-49 1.1587676634398179 1102 50-51 1.1091557831737866 1102 52-53 1.251960043326239 1102 54-55 1.320745118873596 1102 56-57 1.22122967684626 1102 58-59 1.0624409001832653 1102 60-61 1.1447082134578963 1102 62-63 1.1900251136309308 1102 64-65 1.0611513569444142 1102 66-67 0.8095161728172613 1102 68-69 0.7305713941887007 1102 70-71 1.1532974240993887 1102 72-73 0.9382465118167005 1102 74-75 0.8240771318418858 1102 76-77 0.5822470055034756 1102 78-79 0.6643049091355984 1102 80-81 0.02040863035307794 1102 82-83 -0.284120325713765 1102 84-85 -0.23899373001599145 1102 86-87 -0.5109150318459896 1102 88-89 -0.7487072449193519 1102 90-91 -4.697483949043249 1102 92-93 -9.985966974021288 1102 94-95 -8.563402061170784 1102 96-97 -5.060958374448127 1102 98-99 -3.8698472052537927 1102 100-101 -3.423329286104904 1103 1 0.826986688404105 1103 2 0.7848074522003223 1103 3 0.738252270038231 1103 4 0.8020473886798953 1103 5 0.935377650177692 1103 6 0.6239935249940771 1103 7 0.7190562661720747 1103 8 0.7060039458336291 1103 9 0.7780693853826222 1103 10-11 0.7099617895232484 1103 12-13 0.780186665944754 1103 14-15 0.823932700731028 1103 16-17 1.0678397910604502 1103 18-19 0.9767777711882317 1103 20-21 1.021981473769955 1103 22-23 0.9626325485598883 1103 24-25 0.7215874898401538 1103 26-27 0.751417373375304 1103 28-29 0.8854496697267606 1103 30-31 0.9721337198974496 1103 32-33 1.0044887337595085 1103 34-35 1.058133525160926 1103 36-37 0.8864802373831822 1103 38-39 1.0144137586007034 1103 40-41 0.7628908906717697 1103 42-43 0.5780327289948559 1103 44-45 0.774046472115451 1103 46-47 0.804024603694856 1103 48-49 0.46629757716169706 1103 50-51 0.8741299079585012 1103 52-53 0.8665693666246206 1103 54-55 0.8785892756786353 1103 56-57 0.6548230639715449 1103 58-59 0.7518906356764958 1103 60-61 0.5911952265249809 1103 62-63 0.9031966052682421 1103 64-65 0.6004701749464942 1103 66-67 0.7815631304756252 1103 68-69 0.8091798460739561 1103 70-71 0.789109215305519 1103 72-73 0.8832188280081326 1103 74-75 0.558841994162723 1103 76-77 0.2834472537740105 1103 78-79 0.22830074937104072 1103 80-81 -0.05037724453336878 1103 82-83 0.2771465938819304 1103 84-85 0.3133412050185136 1103 86-87 -0.2318844378464071 1103 88-89 -0.3013229229568637 1103 90-91 -1.683266350608502 1103 92-93 -8.456529241010763 1103 94-95 -5.27463013711526 1103 96-97 -2.7262148864115616 1103 98-99 -1.916253653710939 1103 100-101 -1.6581899216610267 1104 1 0.7535607390927979 1104 2 0.7438331402098441 1104 3 0.8375558356077235 1104 4 0.9048823797143086 1104 5 0.7310652819673322 1104 6 0.5803854569311824 1104 7 0.721721129599679 1104 8 0.7766265299216215 1104 9 0.648560577927519 1104 10-11 0.6812060022590387 1104 12-13 0.4574834071530418 1104 14-15 0.7195016061150383 1104 16-17 0.8107212061192257 1104 18-19 0.6514137225511405 1104 20-21 0.6894075549171959 1104 22-23 0.3533620382585312 1104 24-25 0.5714348786369143 1104 26-27 0.50889073029591 1104 28-29 0.5847067223937401 1104 30-31 0.6111698955295779 1104 32-33 0.587159122445911 1104 34-35 0.6129339455546159 1104 36-37 0.6302148827556309 1104 38-39 0.4962385456819618 1104 40-41 0.3765397612155468 1104 42-43 0.35527629143879835 1104 44-45 0.4552164493700488 1104 46-47 0.2930107483307225 1104 48-49 0.2744851811769209 1104 50-51 0.548574030631098 1104 52-53 0.46502377546266516 1104 54-55 0.7636383401249276 1104 56-57 0.5590209274887634 1104 58-59 0.44267167470659885 1104 60-61 0.40060971944176416 1104 62-63 0.39181723848067307 1104 64-65 0.6202218641869806 1104 66-67 0.6672632274268828 1104 68-69 0.8156698032124616 1104 70-71 0.7458274041464215 1104 72-73 0.5968337767217342 1104 74-75 0.262132263559824 1104 76-77 0.25821844806684524 1104 78-79 -0.07673871623422102 1104 80-81 -0.04247560599046807 1104 82-83 -0.19318698806335988 1104 84-85 -0.3703336549798735 1104 86-87 -0.24742330241313226 1104 88-89 -0.36806486448540454 1104 90-91 -0.9364165258095731 1104 92-93 -4.012472856707532 1104 94-95 -1.8149952634000108 1104 96-97 -0.8195262125255169 1104 98-99 -1.022409725237118 1104 100-101 -1.004736597877157 1105 1 0.8161040158888007 1105 2 0.8585721797531782 1105 3 0.8310603694372354 1105 4 0.8049229855564093 1105 5 0.7641491304612842 1105 6 0.5058770409481923 1105 7 0.7318204560420085 1105 8 0.6833131299203004 1105 9 1.0618876649613327 1105 10-11 0.9719886229586194 1105 12-13 0.861210112947191 1105 14-15 1.0903467583428963 1105 16-17 1.1352966254130976 1105 18-19 0.8657138078013773 1105 20-21 0.7921429054234466 1105 22-23 0.8815416115373935 1105 24-25 1.0314524426828982 1105 26-27 0.9102027469083325 1105 28-29 0.9454620938564275 1105 30-31 1.05315567540773 1105 32-33 1.155824849720041 1105 34-35 1.1551867841085794 1105 36-37 1.1771142632411866 1105 38-39 1.0326696225885499 1105 40-41 1.0483513031140888 1105 42-43 1.3532559121523278 1105 44-45 1.274022608240955 1105 46-47 1.3106658453919025 1105 48-49 1.2976664910347395 1105 50-51 1.3226223449746506 1105 52-53 1.1720963145320162 1105 54-55 1.2516151925920838 1105 56-57 1.2536649948871208 1105 58-59 1.17455453015641 1105 60-61 1.128084674889152 1105 62-63 1.190516162682858 1105 64-65 1.0787969295565105 1105 66-67 1.070401772650996 1105 68-69 0.8828397963028607 1105 70-71 1.1052059952485287 1105 72-73 1.0018025219718396 1105 74-75 0.9437435444942466 1105 76-77 0.7430140200474931 1105 78-79 0.7293988604708019 1105 80-81 0.7034456362986425 1105 82-83 0.5387971984209905 1105 84-85 0.4821177116306714 1105 86-87 0.31837064459955045 1105 88-89 0.10373353374996697 1105 90-91 -1.3209556862158642 1105 92-93 -7.341840205939068 1105 94-95 -3.169092525038945 1105 96-97 -1.20537202927936 1105 98-99 -0.5743738217424479 1105 100-101 -0.25325353007274387 1106 1 0.7325672220337864 1106 2 0.5654486931079177 1106 3 0.549868495246006 1106 4 0.62628994563525 1106 5 0.5112212760503709 1106 6 0.53546843226151 1106 7 0.7212952991725956 1106 8 0.6379367169712964 1106 9 0.6599856159865993 1106 10-11 0.6479746731521772 1106 12-13 0.7381549409665169 1106 14-15 0.556760459422236 1106 16-17 1.096883873342371 1106 18-19 0.838217368522038 1106 20-21 0.8745783375596403 1106 22-23 0.8481677540887276 1106 24-25 0.7922448958934396 1106 26-27 0.5802392728854997 1106 28-29 0.5804942353207352 1106 30-31 0.5030574793769702 1106 32-33 0.5537067927376569 1106 34-35 0.6229297950555122 1106 36-37 0.5774338558497689 1106 38-39 0.2773362949409943 1106 40-41 0.6255978135303124 1106 42-43 0.6971742322830181 1106 44-45 0.34710518827285597 1106 46-47 0.35760029169112073 1106 48-49 0.4105888250687144 1106 50-51 0.14579311923077753 1106 52-53 0.3255030926555058 1106 54-55 0.528410536247172 1106 56-57 0.4916184391913987 1106 58-59 0.4271334843396062 1106 60-61 -0.017975815106328952 1106 62-63 0.17608060959458527 1106 64-65 0.3028203384357866 1106 66-67 0.3401338250294472 1106 68-69 0.36451555634323896 1106 70-71 0.3663585910274527 1106 72-73 0.34352858567172007 1106 74-75 0.14133233378719368 1106 76-77 0.2524327755281348 1106 78-79 -0.06531444821208154 1106 80-81 0.2218838503326488 1106 82-83 0.3863274112423767 1106 84-85 0.17000203492317212 1106 86-87 -0.0848738949180543 1106 88-89 -0.2616068726955376 1106 90-91 -0.8436055136653202 1106 92-93 -4.25732881744797 1106 94-95 -2.0121928679691017 1106 96-97 -1.1562933972473672 1106 98-99 -0.8156832985704341 1106 100-101 -1.085277841440611 1107 1 0.48079942895072136 1107 2 0.6102342849003755 1107 3 0.5850788224883985 1107 4 0.6269951124071866 1107 5 0.41650912662702666 1107 6 0.6156653988441079 1107 7 0.5908859573632554 1107 8 0.3173116391403852 1107 9 0.570494701385293 1107 10-11 0.4442482418511844 1107 12-13 0.5609506571533913 1107 14-15 0.45389425656525617 1107 16-17 0.6670629706989004 1107 18-19 0.7480587960196416 1107 20-21 0.5457493194968706 1107 22-23 0.7328381036263849 1107 24-25 0.8058864971321427 1107 26-27 0.506758644716939 1107 28-29 0.294012732445303 1107 30-31 0.41395553176636923 1107 32-33 0.30704208153346 1107 34-35 0.35665882197038457 1107 36-37 0.2383821701260942 1107 38-39 0.2777537686735343 1107 40-41 0.36127130030993726 1107 42-43 0.354375785450344 1107 44-45 0.29814298371147885 1107 46-47 0.11575209459250857 1107 48-49 0.049260996037148175 1107 50-51 0.1045990018220131 1107 52-53 0.23196572106712665 1107 54-55 0.5876265408322574 1107 56-57 0.19930768269749066 1107 58-59 0.14389124755060578 1107 60-61 0.2948324140398668 1107 62-63 0.11446778816231529 1107 64-65 0.03369869116620805 1107 66-67 0.2482892287824363 1107 68-69 0.29355252358599415 1107 70-71 0.2453410953897368 1107 72-73 0.40212306826685307 1107 74-75 0.12297549239420746 1107 76-77 0.003191955074097308 1107 78-79 0.04486298375057629 1107 80-81 -0.10640016383669959 1107 82-83 -0.1768315258462465 1107 84-85 -0.026836267335312414 1107 86-87 -0.34570420628669396 1107 88-89 -0.7738726485515315 1107 90-91 -1.0112615393153845 1107 92-93 -3.676347540375943 1107 94-95 -1.5374610939045361 1107 96-97 -0.819328705097675 1107 98-99 -0.559955318903242 1107 100-101 -0.6056849177141501 1108 1 0.3352814547061165 1108 2 0.34179652710174935 1108 3 0.14864095283138568 1108 4 -0.06940304752234283 1108 5 -0.46001179788254376 1108 6 0.18788676659253412 1108 7 0.24638869090259163 1108 8 0.28386877780283726 1108 9 0.21050504828611594 1108 10-11 0.1488085684776692 1108 12-13 0.1809446447650771 1108 14-15 0.22091281107524452 1108 16-17 0.3912208867771412 1108 18-19 0.260038829297514 1108 20-21 0.2690668994406593 1108 22-23 0.3914204383905755 1108 24-25 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2.0475811185881447 2206 90-91 2.7908920201452254 2206 92-93 3.7664398845813665 2206 94-95 3.1830013782556676 2206 96-97 2.6817042213997944 2206 98-99 2.043220434463173 2206 100-101 1.4704235129075087 2207 1 0.7728921012282655 2207 2 0.628317861585419 2207 3 0.6328851598108614 2207 4 0.7650969287009701 2207 5 0.6377497966501764 2207 6 0.6560844256076379 2207 7 0.5120198261376103 2207 8 0.4618074067710012 2207 9 0.47675777411371456 2207 10-11 0.5613704727866917 2207 12-13 0.6912895426272101 2207 14-15 0.9631018289621096 2207 16-17 1.1338052770409277 2207 18-19 0.9290768244470655 2207 20-21 0.7283364116109254 2207 22-23 1.082847922884774 2207 24-25 0.9884692176585546 2207 26-27 1.1042921277155031 2207 28-29 0.8619034884878474 2207 30-31 0.8396516566565779 2207 32-33 0.6564149941003521 2207 34-35 0.9119190524942837 2207 36-37 1.2840242352909996 2207 38-39 1.2224535213089354 2207 40-41 1.151104198053794 2207 42-43 0.9638131015914553 2207 44-45 1.0560730436896577 2207 46-47 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0.3414637262900939 2311 76-77 0.37351115097208876 2311 78-79 0.42111311108936533 2311 80-81 0.42922379622985574 2311 82-83 0.8326221058560179 2311 84-85 0.9208244342165237 2311 86-87 1.145434711842956 2311 88-89 1.0283756451086958 2311 90-91 1.9606067753981868 2311 92-93 2.8426218643576675 2311 94-95 2.1240880249110425 2311 96-97 1.9349522552065785 2311 98-99 1.6039626409595726 2311 100-101 1.4612185425052147 2312 1 0.3101123626790603 2312 2 0.006734697491854291 2312 3 0.05378544965450516 2312 4 -0.04156947506896813 2312 5 0.021500807159476665 2312 6 0.1894854548483309 2312 7 0.08320634479341749 2312 8 -0.510986725374039 2312 9 0.1975174142080931 2312 10-11 0.03490203025262417 2312 12-13 -0.01426612246864778 2312 14-15 -0.27829126079740263 2312 16-17 0.23420783765505604 2312 18-19 0.17142094311864753 2312 20-21 0.47671532466440425 2312 22-23 0.11143136959233146 2312 24-25 0.1970928875654252 2312 26-27 0.0063676973798507674 2312 28-29 -0.0241297942378651 2312 30-31 0.18685706444225758 2312 32-33 0.2288627598067947 2312 34-35 0.02835437004067387 2312 36-37 0.16242069670104797 2312 38-39 0.36724553713531805 2312 40-41 0.40787181706385667 2312 42-43 0.4389279545993219 2312 44-45 0.42452313603212843 2312 46-47 0.48656361468774634 2312 48-49 0.17523396791328594 2312 50-51 0.19477594269291743 2312 52-53 0.13797129443400635 2312 54-55 0.14402373791570966 2312 56-57 0.19770060506264997 2312 58-59 0.2991649783245194 2312 60-61 0.42899904688755797 2312 62-63 0.12943674234437097 2312 64-65 0.08596929706694567 2312 66-67 0.15195250002413374 2312 68-69 -0.03351753580012584 2312 70-71 0.24059561698653553 2312 72-73 0.2147463384263446 2312 74-75 0.19972156601134827 2312 76-77 0.41236298438838404 2312 78-79 0.5608891190535239 2312 80-81 0.48182542224611424 2312 82-83 0.5615834464898306 2312 84-85 0.7981464849880524 2312 86-87 0.7786353091550531 2312 88-89 1.131946244079991 2312 90-91 1.6981793654098034 2312 92-93 2.9663583833555087 2312 94-95 2.1351665050835997 2312 96-97 1.5803297232669706 2312 98-99 1.6118504790216264 2312 100-101 1.8684327452594829 2313 1 -0.38472075670476613 2313 2 -1.1403021018235044 2313 3 -0.5758960330155851 2313 4 -0.35299437879169204 2313 5 -0.9510320875773672 2313 6 -0.402920674805074 2313 7 -1.2563368730071787 2313 8 -1.2187932080441328 2313 9 -1.1058054890867268 2313 10-11 -2.1509391924688117 2313 12-13 -0.6491713160929535 2313 14-15 -0.21513871479825752 2313 16-17 -2.0919468210954832 2313 18-19 -1.3536124864919614 2313 20-21 -1.7979644196659308 2313 22-23 -1.3653651376819553 2313 24-25 -0.9746711261273404 2313 26-27 -1.6669588163129205 2313 28-29 -2.814463956625538 2313 30-31 -1.1462135121516681 2313 32-33 -0.9325387431884948 2313 34-35 -0.5817928539644655 2313 36-37 -0.6688680816472612 2313 38-39 -1.0984489964547635 2313 40-41 -1.1515499843909964 2313 42-43 -0.6158137013698664 2313 44-45 -1.0602256070145089 2313 46-47 -1.4339608290435386 2313 48-49 -0.9042970815090463 2313 50-51 -0.9618411144315999 2313 52-53 -0.7997419864816955 2313 54-55 -0.7648123767612276 2313 56-57 -1.1621136600207898 2313 58-59 -0.7101438310464694 2313 60-61 -0.6414237708274619 2313 62-63 -0.4961770253064621 2313 64-65 -0.5693881323297205 2313 66-67 -0.7889205476866046 2313 68-69 -0.5877370212515594 2313 70-71 -0.7212859352171499 2313 72-73 -0.35814559032846915 2313 74-75 -0.2074530970181776 2313 76-77 -0.23702993388290494 2313 78-79 -0.22375866656906496 2313 80-81 -0.16005251955961697 2313 82-83 -0.19831572980456258 2313 84-85 0.03801931874928499 2313 86-87 0.3053803348289925 2313 88-89 0.5212166762579997 2313 90-91 1.3356185235184874 2313 92-93 2.6785160641428405 2313 94-95 1.8078645420968655 2313 96-97 0.9475626875374061 2313 98-99 1.0680550147511951 2313 100-101 0.9671624200562334 2314 1 -0.1885031086241682 2314 2 -0.41572767069618166 2314 3 -1.5292218754682523 2314 4 -0.30361970448215203 2314 5 -0.18245958888012836 2314 6 -2.8796202362085843 2314 7 -3.9205608611954723 2314 8 -1.1707267237641261 2314 9 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-0.051075121768455745 2314 74-75 0.32016952544143606 2314 76-77 0.1511116763307463 2314 78-79 0.24132460162617164 2314 80-81 0.38184755860609343 2314 82-83 0.6052142120054107 2314 84-85 0.5120984289629362 2314 86-87 0.8461974922026236 2314 88-89 1.0139212098692525 2314 90-91 1.5012361954830524 2314 92-93 2.206558013074577 2314 94-95 1.4904832562709132 2314 96-97 1.5888159987237884 2314 98-99 1.5250638735318134 2314 100-101 1.5378101098252088 2315 1 -7.197008443650546 2315 2 -2.817746183528186 2315 3 -1.2460366835207246 2315 4 -0.7072759334747118 2315 5 -0.8790021266209109 2315 6 -1.5675218847137486 2315 7 -2.6921935148212697 2315 8 -2.4151676179792787 2315 9 -2.975222620820084 2315 10-11 -1.9300643386063712 2315 12-13 -2.418538333673574 2315 14-15 -4.391609465228399 2315 16-17 -4.010693994850278 2315 18-19 -2.768125462404086 2315 20-21 -3.9056874448787973 2315 22-23 -5.372434541992185 2315 24-25 -3.476659148362309 2315 26-27 -4.347350809629198 2315 28-29 -2.5532110200878435 2315 30-31 -2.1255912281043763 2315 32-33 -1.4125141066022735 2315 34-35 -1.8773302127900706 2315 36-37 -2.472254474862069 2315 38-39 -1.8523705840049445 2315 40-41 -3.364131876569054 2315 42-43 -7.537358513210869 2315 44-45 -3.8639342611151832 2315 46-47 -2.4521404789751564 2315 48-49 -5.231499415849719 2315 50-51 -6.275587764297043 2315 52-53 -6.724535946313168 2315 54-55 -4.357524248645461 2315 56-57 -3.5146550059201154 2315 58-59 -3.942382258606358 2315 60-61 -2.9024261366014947 2315 62-63 -2.8427291440253057 2315 64-65 -1.446322731637082 2315 66-67 -2.178053497018233 2315 68-69 -1.787914798968064 2315 70-71 -1.4175276494934081 2315 72-73 -2.0625889303161173 2315 74-75 -2.355607283169853 2315 76-77 -2.3641599577060823 2315 78-79 -1.3260928693749072 2315 80-81 -1.1649757008363615 2315 82-83 -1.7503482826346648 2315 84-85 -1.6144473039842069 2315 86-87 -2.1419543091468327 2315 88-89 -1.4133743591679497 2315 90-91 -0.33081940110762176 2315 92-93 0.6088801396711432 2315 94-95 0.4550102412409949 2315 96-97 0.22848835866724215 2315 98-99 0.14152084613602867 2315 100-101 0.3059878601232917 2316 1 -3.9360819182292666 2316 2 -2.747561629954177 2316 3 -4.446614045719759 2316 4 -4.166106901477715 2316 5 -3.731947468702593 2316 6 -4.648298397128123 2316 7 -3.1438822450579984 2316 8 -1.5743172552310654 2316 9 -1.5492095271992454 2316 10-11 -2.990455412976985 2316 12-13 -4.322217482154656 2316 14-15 -3.0753020541812006 2316 16-17 -3.1071889944167097 2316 18-19 -3.5869439293231764 2316 20-21 -6.3017785076877075 2316 22-23 -7.291950323707365 2316 24-25 -4.360515046272532 2316 26-27 -4.098654029561562 2316 28-29 -4.689846783304308 2316 30-31 -6.706736992187963 2316 32-33 -4.4972761524444 2316 34-35 -6.286037976469004 2316 36-37 -9.518817038090095 2316 38-39 -6.114153397543689 2316 40-41 -5.679442452630564 2316 42-43 -4.762649000825405 2316 44-45 -3.525280620626127 2316 46-47 -5.649892090386551 2316 48-49 -3.2756701531968844 2316 50-51 -4.403268632580421 2316 52-53 -4.95150866071037 2316 54-55 -7.103582794184092 2316 56-57 -5.199860370547103 2316 58-59 -5.69703981561997 2316 60-61 -6.158648135618748 2316 62-63 -6.619900936377242 2316 64-65 -5.164955850115561 2316 66-67 -6.310123474183882 2316 68-69 -5.380633459545567 2316 70-71 -5.925990449736201 2316 72-73 -7.840583770909234 2316 74-75 -7.657711150557198 2316 76-77 -6.754713690688714 2316 78-79 -5.538385481451101 2316 80-81 -5.595431376183939 2316 82-83 -4.73857775339803 2316 84-85 -6.2075200415048 2316 86-87 -4.687218349381535 2316 88-89 -4.817394938987011 2316 90-91 -5.4534326334688075 2316 92-93 -3.8397461863108795 2316 94-95 -3.634657576777908 2316 96-97 -4.329345774350072 2316 98-99 -5.393991264740638 2316 100-101 -4.64770826959894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5401.0 3 2042.0 4 2144.0 5 2634.0 6 3091.0 7 3703.0 8 4612.0 9 5879.0 10 6936.0 11 8506.0 12 10341.0 13 11804.0 14 12908.0 15 14635.0 16 16572.0 17 18771.0 18 16561.0 19 15080.0 20 15631.0 21 17151.0 22 18782.0 23 20975.0 24 23377.0 25 26482.0 26 29458.0 27 33658.0 28 38084.0 29 43234.0 30 49306.0 31 55707.0 32 64991.0 33 75191.0 34 88407.0 35 104761.0 36 117481.0 37 98316.0 38 29175.0 39 409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98323018872 18.86660013336657 13.721638231030136 22.428531446883298 2 15.867394464119375 21.35017527438654 39.21718273986041 23.565247521633676 3 19.213981266154523 26.308831258594743 30.165487101581796 24.31170037366894 4 12.753428319171858 16.88199130061479 37.39313643166807 32.971443948545286 5 13.358052468163613 37.793457228996985 33.903343382981596 14.945146919857805 6 30.50511467526671 36.39589962134846 17.920529968181754 15.178455735203075 7 26.88879229426921 33.73705084626786 21.965616515160104 17.40854034430282 8 26.0097405928227 33.16589064690711 20.815837642387777 20.008531117882413 9 27.08740336532967 16.02126570158728 20.995380972992237 35.89594996009082 10-11 24.681863771414378 26.272983629837515 27.69878649044253 21.34636610830558 12-13 26.335559284222025 24.33017866551837 27.92603201643613 21.408230033823475 14-15 23.467863759313097 25.30116686833706 26.99803309494378 24.23293627740606 16-17 21.625532772722035 28.41375021598894 27.540479927427715 22.420237083861306 18-19 21.587423726983097 26.796533290134455 30.19592490595244 21.42011807693 20-21 22.855121588562806 25.80167986448757 29.906790202350585 21.436408344599034 22-23 22.37558728974251 25.79421106379838 30.052312050404637 21.777889596054475 24-25 22.409136090582997 25.968062578330958 30.123609850612965 21.499191480473083 26-27 21.916114070745184 25.9246536642861 30.571726868069852 21.58750539689886 28-29 21.742916055140867 26.53321147837675 30.30928948955979 21.414582976922592 30-31 21.93576416245346 26.143598701596893 29.951377414809176 21.969259721140467 32-33 21.739936856767144 26.289166834361332 30.003074777416277 21.967821531455243 34-35 22.384566559077342 26.24756348445357 29.781220035923127 21.58664992054596 36-37 22.43338099336961 25.914289367249992 29.956493709955666 21.69583592942473 38-39 22.034176791814712 26.402586780070123 29.86721729825893 21.696019129856232 40-41 22.070206647953057 26.356166578740307 29.95967691113796 21.613949862168678 42-43 21.800685466119663 26.525081091884328 29.26991646388069 22.404316978115318 44-45 21.965219575229984 26.35181816995211 29.334579188546773 22.348383066271126 46-47 22.585152775520623 26.56641446051939 28.913520767503808 21.93491199645618 48-49 22.211430874211754 26.80008014714656 28.724106955916817 22.264382022724867 50-51 21.951927922053653 26.98028150494415 28.850271557661593 22.21751901534061 52-53 22.27320627600742 27.225289571812006 28.164348891250697 22.337155260929872 54-55 21.95755151374323 27.008075668923244 28.273082169357128 22.7612906479764 56-57 22.210357282254467 27.164084766692614 27.86547679126204 22.76008115979088 58-59 22.3447245161392 26.84915967237689 28.16676457546968 22.639351236014235 60-61 22.08346105872307 27.094662631669685 28.630518243236857 22.19135806637039 62-63 21.883214622658496 26.94454747106652 28.70936349952828 22.462874406746703 64-65 21.808815626859317 27.416898373387316 28.240106070521055 22.534179929232312 66-67 21.831017614110014 27.26095457925449 27.91196365908244 22.996064147553057 68-69 21.81113228625879 27.08380407277009 28.260570144602156 22.84449349636897 70-71 22.351607700469092 26.872372021586955 27.955095751705922 22.820924526238034 72-73 21.970130194097905 26.864093643930804 28.055580821293585 23.11019534067771 74-75 21.71505670035077 27.80881887366682 27.596077158051692 22.880047267930717 76-77 21.827378933815037 27.152088824878103 27.80857471360651 23.211957527700353 78-79 21.80841242291074 27.184191872200493 27.963843821015118 23.043551883873654 80-81 22.110855350121085 27.18415556913676 27.59115632440982 23.113832756332336 82-83 22.060709782021256 26.948567825617005 27.842776076382634 23.147946315979105 84-85 21.732879586621014 27.19702140918432 27.650077715681277 23.420021288513386 86-87 21.833986221801972 27.056823810167046 27.802107253816022 23.307082714214957 88-89 21.873482847108818 26.974629375592784 27.414001356486683 23.737886420811712 90-91 21.61618693150854 26.86671646049707 27.747499302450642 23.769597305543748 92-93 21.47647487701919 26.88048882245336 28.058067102796723 23.584969197730725 94-95 21.850199194574774 26.644244704432722 27.681470626118582 23.82408547487392 96-97 21.64920174851869 26.493571423745532 27.910673305812857 23.94655352192292 98-99 21.761239751328947 26.68893594017479 27.31867735832057 24.23114695017569 100-101 21.801400955475422 26.35892140484802 27.706309345529974 24.13336829414659 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2542.0 1 1674.0 2 885.0 3 924.5 4 785.0 5 793.0 6 1105.0 7 1774.0 8 2634.0 9 2958.5 10 2541.5 11 2097.0 12 2032.5 13 2047.5 14 2084.5 15 2212.0 16 2331.5 17 2423.5 18 2575.5 19 2776.0 20 3005.5 21 3419.0 22 3919.0 23 4393.5 24 4812.5 25 5560.5 26 6649.0 27 7813.5 28 8871.0 29 10046.0 30 11783.5 31 14021.0 32 16326.5 33 17831.0 34 19110.5 35 21183.5 36 24046.0 37 26788.5 38 28935.0 39 30092.0 40 30038.5 41 29912.0 42 29852.0 43 30932.0 44 33147.0 45 35373.0 46 37624.0 47 40843.0 48 45776.0 49 46104.5 50 41855.5 51 39283.0 52 38434.0 53 38431.5 54 37739.0 55 35988.5 56 33523.0 57 30437.5 58 28060.5 59 25584.0 60 22008.5 61 18516.5 62 15138.5 63 11461.0 64 8286.0 65 6034.0 66 4384.5 67 3328.0 68 2478.0 69 1681.0 70 1147.0 71 774.0 72 518.0 73 328.0 74 216.5 75 143.0 76 88.0 77 61.0 78 37.5 79 21.0 80 15.0 81 12.0 82 8.0 83 3.0 84 2.0 85 4.0 86 7.0 87 5.0 88 4.5 89 5.0 90 2.0 91 1.0 92 0.5 93 0.5 94 1.0 95 0.5 96 0.5 97 1.0 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0874845800560333 2 0.09719509870562383 3 0.09458764462378934 4 0.09737492312506069 5 0.09404817136547874 6 0.07948239339109293 7 0.09198019054195485 8 0.087214843426878 9 0.08298896957011175 10-11 0.08968742919413485 12-13 0.08860848267751367 14-15 0.09526198619667757 16-17 0.09242975159054699 18-19 0.09494729346266306 20-21 0.10375869001506928 22-23 0.11387381360839276 24-25 0.13702620761088874 26-27 0.14426414049322242 28-29 0.14260076461343144 30-31 0.1439494477592079 32-33 0.13904923232955343 34-35 0.1340591046901805 36-37 0.1395437494830048 38-39 0.1323058166006711 40-41 0.12749551338073506 42-43 0.1275854255904535 44-45 0.1180097752554406 46-47 0.13761063697405854 48-49 0.15631237659549216 50-51 0.165663246406209 52-53 0.17407003801488227 54-55 0.1695294714241015 56-57 0.13913914453927187 58-59 0.1320360799715158 60-61 0.12718082064672054 62-63 0.12335955173368722 64-65 0.11122140342169906 66-67 0.12425867383087154 68-69 0.12183104416847389 70-71 0.1287992404216523 72-73 0.15977399666965175 74-75 0.17348560865171248 76-77 0.17681236041129442 78-79 0.17595819441896932 80-81 0.1799592877514395 82-83 0.17946477059798813 84-85 0.15626742049063294 86-87 0.1569867181683804 88-89 0.17757661419390108 90-91 0.2657355358228226 92-93 0.5419458440778424 94-95 0.17757661419390108 96-97 0.19002945523990375 98-99 0.20643843351351743 100-101 0.21417088354930247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1112196.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.427510731310605 #Duplication Level Percentage of deduplicated Percentage of total 1 76.92273751062085 41.86713121341105 2 11.840684930378796 12.889180122285193 3 4.425383185755835 7.225877724986615 4 2.1829683358683267 4.752541301063384 5 1.2326285122684044 3.3544450789604006 6 0.7763352361808423 2.5352396638996404 7 0.5242430676709856 1.997327163403443 8 0.36136233050202965 1.5734441697032504 9 0.278392271203607 1.3636978495603425 >10 1.34558241654317 12.73964126456783 >50 0.06910918408625083 2.5695256821625794 >100 0.036381592736647275 3.557534009140114 >500 0.0025148557105621754 0.9961020011192555 >1k 0.0015089134263373053 1.9136364112744508 >5k 1.6765704737081168E-4 0.6646763444625006 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7284 0.6549205355890508 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4969 0.4467737700908833 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3543 0.3185589590324007 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3387 0.30453265431632554 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1886 0.1695744275289607 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1690 0.15195163442414825 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1620 0.1456577797438581 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1590 0.14296041345230517 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1217 0.10942315922733044 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 8.991220971843092E-5 5 0.0 0.0 0.0 0.0 8.991220971843092E-5 6 0.0 0.0 0.0 0.0 8.991220971843092E-5 7 0.0 0.0 0.0 0.0 8.991220971843092E-5 8 0.0 0.0 0.0 0.0 8.991220971843092E-5 9 0.0 0.0 0.0 0.0 8.991220971843092E-5 10-11 0.0 0.0 0.0 0.0 8.991220971843092E-5 12-13 0.0 0.0 0.0 0.0 8.991220971843092E-5 14-15 0.0 0.0 0.0 1.7982441943686185E-4 1.7982441943686185E-4 16-17 4.495610485921546E-5 0.0 0.0 3.1469273401450823E-4 2.6973662915529277E-4 18-19 8.991220971843092E-5 0.0 0.0 5.394732583105855E-4 2.6973662915529277E-4 20-21 8.991220971843092E-5 0.0 0.0 8.541659923250938E-4 2.6973662915529277E-4 22-23 8.991220971843092E-5 0.0 0.0 0.0013037270409172485 3.1469273401450823E-4 24-25 1.7982441943686185E-4 0.0 0.0 0.0022028491381015577 3.596488388737237E-4 26-27 1.7982441943686185E-4 0.0 0.0 0.0034166639693003755 3.596488388737237E-4 28-29 2.247805242960773E-4 0.0 0.0 0.009171045391279954 3.596488388737237E-4 30-31 2.6973662915529277E-4 0.0 0.0 0.02369186726080655 3.596488388737237E-4 32-33 3.596488388737237E-4 4.495610485921546E-5 0.0 0.05592539444486404 3.596488388737237E-4 34-35 4.495610485921546E-4 8.991220971843092E-5 0.0 0.10704048566979202 3.596488388737237E-4 36-37 7.192976777474474E-4 8.991220971843092E-5 0.0 0.17478933569262972 3.596488388737237E-4 38-39 7.192976777474474E-4 8.991220971843092E-5 0.0 0.2730633809148747 3.596488388737237E-4 40-41 7.192976777474474E-4 8.991220971843092E-5 0.0 0.38046351542354045 3.596488388737237E-4 42-43 8.092098874658783E-4 8.991220971843092E-5 0.0 0.4918197871598171 3.596488388737237E-4 44-45 8.991220971843092E-4 8.991220971843092E-5 0.0 0.6261486284791529 3.596488388737237E-4 46-47 9.890343069027402E-4 8.991220971843092E-5 0.0 0.7820564001309122 3.596488388737237E-4 48-49 9.890343069027402E-4 8.991220971843092E-5 0.0 0.9402119770256321 3.596488388737237E-4 50-51 9.890343069027402E-4 8.991220971843092E-5 0.0 1.1221043772860178 3.596488388737237E-4 52-53 0.0010339904117619555 8.991220971843092E-5 0.0 1.2928926196461776 4.0460494373293916E-4 54-55 0.001078946516621171 8.991220971843092E-5 0.0 1.481213742901431 4.495610485921546E-4 56-57 0.001078946516621171 8.991220971843092E-5 0.0 1.7072080820287072 4.495610485921546E-4 58-59 0.001078946516621171 8.991220971843092E-5 0.0 1.9417890371840936 4.495610485921546E-4 60-61 0.001078946516621171 8.991220971843092E-5 0.0 2.2005114206488785 4.495610485921546E-4 62-63 0.001078946516621171 8.991220971843092E-5 0.0 2.471012303586778 5.394732583105855E-4 64-65 0.001078946516621171 8.991220971843092E-5 0.0 2.7434013429287645 5.394732583105855E-4 66-67 0.0011239026214803867 8.991220971843092E-5 0.0 3.053014037094181 5.394732583105855E-4 68-69 0.001168858726339602 8.991220971843092E-5 0.0 3.4152253739448803 5.394732583105855E-4 70-71 0.001168858726339602 8.991220971843092E-5 0.0 3.8086362475678746 5.394732583105855E-4 72-73 0.001168858726339602 8.991220971843092E-5 0.0 4.19566335430086 5.394732583105855E-4 74-75 0.001168858726339602 8.991220971843092E-5 0.0 4.601616981179577 6.293854680290165E-4 76-77 0.001168858726339602 8.991220971843092E-5 0.0 5.040028915766646 6.293854680290165E-4 78-79 0.001168858726339602 8.991220971843092E-5 0.0 5.537378303824146 6.293854680290165E-4 80-81 0.001258770936058033 8.991220971843092E-5 0.0 6.062375696370064 6.743415728882319E-4 82-83 0.001258770936058033 8.991220971843092E-5 0.0 6.57887638509759 7.192976777474474E-4 84-85 0.0013037270409172485 8.991220971843092E-5 0.0 7.099378167157587 7.192976777474474E-4 86-87 0.0013486831457764639 8.991220971843092E-5 0.0 7.658002726138198 7.192976777474474E-4 88-89 0.0013486831457764639 8.991220971843092E-5 0.0 8.249535153875755 7.192976777474474E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5075 0.0 28.707825 1 TACATGG 5355 0.0 27.118141 2 ACATGGG 5355 0.0 26.231928 3 CATGGGA 3195 0.0 25.698769 4 GTATCAA 10725 0.0 24.823528 1 GAGTACT 5065 0.0 23.003267 12-13 ATGGGAT 1305 0.0 22.184843 5 GGTATCA 7960 0.0 21.701326 1 TATCAAC 12330 0.0 21.553753 2 CATGGGG 2190 0.0 21.454979 4 TCAACGC 12360 0.0 21.388176 4 CAACGCA 12395 0.0 21.327782 5 ATCAACG 12515 0.0 21.23514 3 AGAGTAC 7745 0.0 20.987305 10-11 ATGGGAG 1270 0.0 20.92769 5 GTACTTT 5550 0.0 20.90756 14-15 AACGCAG 12600 0.0 20.864023 6 AGTACTT 5275 0.0 20.468048 12-13 ACGCAGA 13585 0.0 19.071808 7 CGCAGAG 13545 0.0 18.885445 8 >>END_MODULE