FastQCFastQC Report
Fri 27 May 2016
SRR939665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112196
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73770.6632823710928649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57800.5196925721725307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40380.3630655028430241No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37590.3379799963315818No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22080.1985261590582955No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18460.1659779391402235No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17020.15303058094076943No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.1517718100047114No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13800.12407884941143467No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11840.10645605630662221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG52450.028.9095421
TACATGG53700.027.9672262
ACATGGG52550.026.6966653
CATGGGA31300.023.9147724
GGTATCA77900.023.3939911
GTATCAA105650.023.1328221
CATGGGG22500.022.1787364
GAGTACT56300.022.04366112-13
AGTACTT57550.020.78885812-13
AGAGTAC84200.020.65750310-11
CGTATAG1150.003914827520.4740121
GTACTTT59650.020.37221714-15
GTACCGG1405.1434455E-420.1815241
TCAACGC122100.019.933724
AACGCAG122500.019.8409376
ATCAACG122850.019.8120233
CAACGCA123400.019.685575
ATGGGGC10350.019.558775
TATCAAC126300.019.2717082
GTGTACG1250.0063404418.8360921
Produced by FastQC (version 0.11.5)