##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1112196 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.528258508392405 31.0 31.0 34.0 30.0 34.0 2 31.72945506007934 31.0 31.0 34.0 30.0 34.0 3 31.757873612205042 31.0 31.0 34.0 30.0 34.0 4 35.560085632388535 37.0 35.0 37.0 33.0 37.0 5 35.291472905854725 37.0 35.0 37.0 33.0 37.0 6 35.321949548460886 37.0 35.0 37.0 32.0 37.0 7 35.2489785972976 37.0 35.0 37.0 32.0 37.0 8 35.21900096745538 37.0 35.0 37.0 32.0 37.0 9 36.81869023085859 39.0 37.0 39.0 32.0 39.0 10-11 36.70163307546512 39.0 35.0 39.0 32.0 39.0 12-13 36.64740162705135 39.0 35.0 39.0 32.0 39.0 14-15 37.654105930969 39.0 36.5 41.0 32.0 41.0 16-17 37.625991282112146 39.0 36.0 41.0 32.0 41.0 18-19 37.64476764886764 39.0 36.5 41.0 32.0 41.0 20-21 37.589101651147814 39.0 36.0 41.0 32.0 41.0 22-23 37.437137878575356 39.0 36.0 41.0 32.0 41.0 24-25 37.29804728662934 39.0 36.0 41.0 31.0 41.0 26-27 37.02367478394096 39.0 36.0 40.0 31.0 41.0 28-29 36.81467699937781 39.0 35.5 40.0 30.5 41.0 30-31 36.539421109228954 38.0 35.0 40.0 30.0 41.0 32-33 36.289067754244755 38.0 35.0 40.0 30.0 41.0 34-35 36.20779700700236 38.0 35.0 40.0 30.0 41.0 36-37 36.05627110689123 38.0 35.0 40.0 30.0 41.0 38-39 36.05705379267683 38.0 35.0 40.0 30.0 41.0 40-41 35.93326491014174 38.0 35.0 40.0 29.5 41.0 42-43 35.67954434290359 38.0 35.0 40.0 28.5 41.0 44-45 35.402174616704244 38.0 34.0 40.0 27.0 41.0 46-47 34.998681437444475 38.0 34.0 40.0 26.0 41.0 48-49 34.61339008592011 38.0 33.5 40.0 24.5 41.0 50-51 34.31010451395258 38.0 33.0 40.0 24.0 41.0 52-53 34.055875942729514 38.0 33.0 40.0 23.5 41.0 54-55 33.73879693866908 37.0 33.0 40.0 22.5 41.0 56-57 31.734734255472958 36.0 30.0 39.5 14.0 40.5 58-59 26.854842132142174 32.5 16.5 37.5 4.0 40.0 60-61 27.569481008743065 32.0 18.5 37.5 2.0 40.0 62-63 30.477623997928422 34.5 29.0 38.0 2.0 40.0 64-65 30.655350315951505 35.0 29.5 38.0 2.0 40.0 66-67 24.175512679419814 26.5 16.5 35.0 2.0 38.5 68-69 16.853664282194863 13.0 7.0 28.0 2.0 34.5 70-71 15.752632629500557 12.0 7.0 26.0 2.0 33.5 72-73 16.85912330200792 13.0 7.0 28.0 2.0 34.0 74-75 17.855050728468726 17.0 7.5 29.0 2.0 34.5 76-77 19.0029216972548 21.0 7.0 30.0 2.0 34.0 78-79 22.06702640541775 27.5 9.0 33.5 2.0 35.5 80-81 25.559731827843294 31.5 22.0 34.5 2.0 36.0 82-83 26.79253342036835 33.0 25.5 35.0 2.0 36.0 84-85 26.8324818647073 33.0 26.0 35.0 2.0 36.0 86-87 26.611975766861235 33.0 25.0 35.0 2.0 35.0 88-89 26.20760594355671 33.0 24.5 35.0 2.0 35.0 90-91 24.958820657510007 32.5 12.0 35.0 2.0 35.0 92-93 22.61760517031171 31.5 2.0 34.0 2.0 35.0 94-95 15.48910129149898 4.5 2.0 32.0 2.0 34.5 96-97 12.99320443518948 2.0 2.0 30.0 2.0 34.0 98-99 12.680028520152923 2.0 2.0 29.5 2.0 34.0 100-101 12.409357703138657 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4037112260593787 1101 2 0.43217188082362057 1101 3 0.3720232839815445 1101 4 0.26581251025314145 1101 5 0.4114158267470387 1101 6 0.44960205560735744 1101 7 0.47854206790309206 1101 8 0.4634720278681499 1101 9 0.5344338357534397 1101 10-11 0.3044748869952727 1101 12-13 0.3139372414462329 1101 14-15 0.4768535152973783 1101 16-17 0.5704427746175611 1101 18-19 0.4988611472181077 1101 20-21 0.27704144263474006 1101 22-23 0.44082007756156827 1101 24-25 0.39904268827361733 1101 26-27 0.4897560928143676 1101 28-29 0.5171772635757819 1101 30-31 0.5076310361928691 1101 32-33 0.4758799349121219 1101 34-35 0.24488790848320008 1101 36-37 0.13986788396677952 1101 38-39 0.37621092343350426 1101 40-41 0.38157013037638166 1101 42-43 0.555733976683193 1101 44-45 0.6393902646403049 1101 46-47 0.7052517436449293 1101 48-49 0.5304575947906258 1101 50-51 0.23816069106501203 1101 52-53 0.4327522803764836 1101 54-55 0.06312034023792279 1101 56-57 -1.9029074753885311 1101 58-59 -9.402430396196987 1101 60-61 -10.0277196558152 1101 62-63 -3.9097361927756182 1101 64-65 -4.875740941728299 1101 66-67 -9.510366404411215 1101 68-69 -4.962664815636293 1101 70-71 -4.643345678445828 1101 72-73 -4.67990563333516 1101 74-75 -6.90586331291059 1101 76-77 -8.113547016063567 1101 78-79 -10.633931463297237 1101 80-81 -7.328280704477191 1101 82-83 -3.7263036176309754 1101 84-85 -3.073250320166835 1101 86-87 -3.0307590987117514 1101 88-89 -2.9625124347821483 1101 90-91 -9.401872494754665 1101 92-93 -16.310927415194627 1101 94-95 -11.019698330729517 1101 96-97 -9.104418255016716 1101 98-99 -8.777894394572172 1101 100-101 -8.813794317593128 1102 1 0.3179055796416179 1102 2 0.3202941736509892 1102 3 0.4597475051723343 1102 4 0.310665721337152 1102 5 0.26757166200410865 1102 6 0.20106668188498134 1102 7 0.41057623028042656 1102 8 0.4064580545748413 1102 9 0.4060883617790054 1102 10-11 0.5815384560990324 1102 12-13 0.3277938867138275 1102 14-15 0.5070816723399005 1102 16-17 0.47309386645953566 1102 18-19 0.5951904046676759 1102 20-21 0.5782291317799491 1102 22-23 0.4844994667500089 1102 24-25 0.4230207943336026 1102 26-27 0.47273118886545973 1102 28-29 0.46064538113742515 1102 30-31 0.6872624936408087 1102 32-33 0.6135507264057836 1102 34-35 0.5508630616347432 1102 36-37 0.5438534946559841 1102 38-39 0.5879980856232265 1102 40-41 0.4053459377358166 1102 42-43 0.6955256416504412 1102 44-45 0.710541491076178 1102 46-47 0.7405422625247908 1102 48-49 0.8526916902788741 1102 50-51 0.9013798507096809 1102 52-53 0.7803454968442551 1102 54-55 0.6145099202233979 1102 56-57 -2.527082985825679 1102 58-59 -9.451605294843706 1102 60-61 -9.70798982246691 1102 62-63 -3.3615784976756515 1102 64-65 -4.409705914687162 1102 66-67 -5.937216662586774 1102 68-69 -3.678571366688164 1102 70-71 -3.931545464727039 1102 72-73 -4.443423346707268 1102 74-75 -5.2064408430718565 1102 76-77 -7.169558468780325 1102 78-79 -9.514598885864963 1102 80-81 -5.058123841412204 1102 82-83 -2.512269961000392 1102 84-85 -2.1330940281062425 1102 86-87 -2.0730631830246296 1102 88-89 -2.483031885639061 1102 90-91 -8.413702074413859 1102 92-93 -13.096604177762838 1102 94-95 -10.352373649472327 1102 96-97 -8.979523279039686 1102 98-99 -8.90852470185411 1102 100-101 -8.816292053968557 1103 1 0.27754928676459656 1103 2 0.21759731443764707 1103 3 0.14671864577812954 1103 4 0.27493038430168326 1103 5 0.23744625250159856 1103 6 0.24235851074272574 1103 7 0.26757404763716863 1103 8 0.26677792895981867 1103 9 0.5121705787855362 1103 10-11 0.3675222691891449 1103 12-13 0.296924076657227 1103 14-15 0.4659069910017024 1103 16-17 0.33753692248892975 1103 18-19 0.33488943830340645 1103 20-21 0.3021670970302992 1103 22-23 0.5145751399372003 1103 24-25 0.43803622903310924 1103 26-27 0.39370717156873525 1103 28-29 0.5044005962572697 1103 30-31 0.4995424309977139 1103 32-33 0.5045024164128975 1103 34-35 0.6339642236559229 1103 36-37 0.4277705788747781 1103 38-39 0.4417564657813102 1103 40-41 0.4604521309346836 1103 42-43 0.4293414035409171 1103 44-45 0.4242350085023858 1103 46-47 0.41715797344581773 1103 48-49 0.3636891705795904 1103 50-51 0.30008177398422475 1103 52-53 0.48903291026812923 1103 54-55 0.2848251498119865 1103 56-57 -2.5388433636572536 1103 58-59 -9.800699970591705 1103 60-61 -10.319325180876664 1103 62-63 -4.273810753936878 1103 64-65 -4.929598796639457 1103 66-67 -6.507468180366477 1103 68-69 -4.07693828916117 1103 70-71 -4.037218281981019 1103 72-73 -4.005728563265762 1103 74-75 -4.612820244838524 1103 76-77 -6.658653473363465 1103 78-79 -8.560980260642484 1103 80-81 -4.9308806719346805 1103 82-83 -2.8095504943709955 1103 84-85 -2.406113926742808 1103 86-87 -2.3371453424850124 1103 88-89 -2.1944091705295 1103 90-91 -5.084673330111954 1103 92-93 -9.489563406315595 1103 94-95 -8.261604874013294 1103 96-97 -7.576973573552662 1103 98-99 -7.616384272806458 1103 100-101 -7.747265036051151 1104 1 0.20769475808917193 1104 2 0.16778629162040204 1104 3 0.11564091664468279 1104 4 0.17399960779543733 1104 5 0.14035427175556947 1104 6 0.19336961088396976 1104 7 0.23622449947149704 1104 8 0.2378429040776382 1104 9 0.2087886908208958 1104 10-11 0.265026866782172 1104 12-13 0.2781696744109823 1104 14-15 0.34672656611967057 1104 16-17 0.3510067776101238 1104 18-19 0.30335250535530633 1104 20-21 0.30738313390880734 1104 22-23 0.30500488804909764 1104 24-25 0.42597731466499056 1104 26-27 0.44342339101779515 1104 28-29 0.4080666721310706 1104 30-31 0.2636738729050947 1104 32-33 0.4912499424523489 1104 34-35 0.4002209645086978 1104 36-37 0.4217194576397816 1104 38-39 0.3411349111986439 1104 40-41 0.2223464355482747 1104 42-43 0.24252358969177124 1104 44-45 0.18983775424494098 1104 46-47 0.23315278296140463 1104 48-49 0.14940208079858053 1104 50-51 0.11655948815651129 1104 52-53 0.12755025965483213 1104 54-55 -0.23032598379188585 1104 56-57 -2.5863113360428116 1104 58-59 -9.802767869060258 1104 60-61 -9.9015700653832 1104 62-63 -4.036740489087375 1104 64-65 -5.049867238301687 1104 66-67 -6.222015979410003 1104 68-69 -3.9812324373492025 1104 70-71 -4.003606044051228 1104 72-73 -4.464556497374458 1104 74-75 -5.10089205542732 1104 76-77 -6.529424261779383 1104 78-79 -9.187827907926554 1104 80-81 -4.851083640020757 1104 82-83 -2.5119708687998568 1104 84-85 -2.2130524223170482 1104 86-87 -2.254274607618182 1104 88-89 -2.085589346552691 1104 90-91 -4.33887560050449 1104 92-93 -6.703781985850068 1104 94-95 -7.740092656599545 1104 96-97 -7.992670789370307 1104 98-99 -8.076546119931583 1104 100-101 -7.986474301895349 1105 1 0.2747788400850695 1105 2 0.3348569362399836 1105 3 0.3005392147973005 1105 4 0.20060634719038006 1105 5 0.16058817021746563 1105 6 0.21677047131662874 1105 7 0.35235941072974697 1105 8 0.09314512388814933 1105 9 0.44899097106288366 1105 10-11 0.4244047970297018 1105 12-13 0.30062073996554517 1105 14-15 0.4719926518706927 1105 16-17 0.4791119680353688 1105 18-19 0.26742514648998394 1105 20-21 0.4293324639858369 1105 22-23 0.5277562018646975 1105 24-25 0.570394598802352 1105 26-27 0.5567996015748733 1105 28-29 0.5813533882933015 1105 30-31 0.5617369081514099 1105 32-33 0.7241361954289474 1105 34-35 0.6679694990636804 1105 36-37 0.6414011019634458 1105 38-39 0.5989515358031383 1105 40-41 0.6254842464895276 1105 42-43 0.4944681542730507 1105 44-45 0.5374579067848586 1105 46-47 0.6457477899848172 1105 48-49 0.5526973954130909 1105 50-51 0.5640042939497079 1105 52-53 0.7306990114106853 1105 54-55 0.6157499718956174 1105 56-57 1.6695807331883117 1105 58-59 -8.056705277320791 1105 60-61 -9.27538172036131 1105 62-63 -2.934549609321053 1105 64-65 -4.069340052580255 1105 66-67 -5.7024789153812705 1105 68-69 -3.1730634671148277 1105 70-71 -3.4304733070045295 1105 72-73 -1.7460493384837257 1105 74-75 -3.70952039301827 1105 76-77 -5.562815407649811 1105 78-79 -8.472243561955967 1105 80-81 -4.040586258840573 1105 82-83 -1.5112483344666288 1105 84-85 -1.0780949042518522 1105 86-87 -0.8123332518529978 1105 88-89 -0.8774950528850702 1105 90-91 -3.387898159876766 1105 92-93 -5.751410142073151 1105 94-95 -7.534705663832966 1105 96-97 -7.538176133848096 1105 98-99 -7.879759415150741 1105 100-101 -8.074442856966197 1106 1 0.21909746507702366 1106 2 0.13586518036469286 1106 3 0.10890925372681437 1106 4 0.037897888995992446 1106 5 -0.03424159360809398 1106 6 0.08306104938227321 1106 7 0.1284724606061971 1106 8 0.1123268830741111 1106 9 0.1950404513178441 1106 10-11 0.1986500676871188 1106 12-13 0.039199491011281395 1106 14-15 0.18435465213895696 1106 16-17 0.13810696480611284 1106 18-19 0.10705199381976627 1106 20-21 0.2264857482612186 1106 22-23 0.14762281343097783 1106 24-25 0.29177450695630114 1106 26-27 0.20842621320317534 1106 28-29 0.3054981369184375 1106 30-31 0.1523273016100788 1106 32-33 0.10819044708835435 1106 34-35 0.3343142729647042 1106 36-37 0.07358652770313512 1106 38-39 -0.008828396541225914 1106 40-41 -0.056321847169250816 1106 42-43 -0.20543461886349235 1106 44-45 0.06771182086391292 1106 46-47 -0.004158355853924434 1106 48-49 -0.1542485103202722 1106 50-51 -0.05685629559864225 1106 52-53 -0.07999664137000906 1106 54-55 -0.28226998054540786 1106 56-57 0.9085436621514518 1106 58-59 -9.078400281147065 1106 60-61 -10.374300362255543 1106 62-63 -4.7379830976953095 1106 64-65 -6.660451858045892 1106 66-67 -7.591860485850976 1106 68-69 -4.414637332782036 1106 70-71 -4.400099603847291 1106 72-73 -4.776811096227565 1106 74-75 -5.34218544027099 1106 76-77 -6.891315826717751 1106 78-79 -9.632831869766601 1106 80-81 -5.9348613488374475 1106 82-83 -3.6043853119206943 1106 84-85 -3.2557432701970193 1106 86-87 -3.1669635843083945 1106 88-89 -3.1932485959668817 1106 90-91 -5.68308442220167 1106 92-93 -7.458885213151881 1106 94-95 -7.575120327876936 1106 96-97 -7.397112670447342 1106 98-99 -6.845200072261676 1106 100-101 -7.25192297546822 1107 1 -5.604840279396228E-4 1107 2 -0.003012164127277117 1107 3 -0.026844352000942706 1107 4 0.07330776343129486 1107 5 -0.14973440765861312 1107 6 -0.04234911012044762 1107 7 -0.06889321637640933 1107 8 0.04116955436587233 1107 9 0.12860849015903142 1107 10-11 0.10783546503359531 1107 12-13 0.07809393954835997 1107 14-15 0.07117350979879689 1107 16-17 -0.09986431540166052 1107 18-19 -0.02518440508857367 1107 20-21 -0.024058130366078956 1107 22-23 0.003855437446553367 1107 24-25 0.077737262147906 1107 26-27 -0.08398431688718944 1107 28-29 -0.03192978978623273 1107 30-31 0.13409123878121676 1107 32-33 0.0893418643985413 1107 34-35 0.03170620615690467 1107 36-37 -0.3037050767613252 1107 38-39 -0.37970148302618156 1107 40-41 -0.29157073353086815 1107 42-43 -0.22803146301713184 1107 44-45 -0.3338986600148175 1107 46-47 -0.28944370083801374 1107 48-49 -0.4324413029157199 1107 50-51 -0.36770918730752555 1107 52-53 -0.4740847182001815 1107 54-55 -0.46530689377538437 1107 56-57 0.6558707800148298 1107 58-59 -8.666047259657486 1107 60-61 -10.212194899313555 1107 62-63 -4.147939302337708 1107 64-65 -5.683753118794062 1107 66-67 -6.777898858926207 1107 68-69 -4.103467866203191 1107 70-71 -4.1767710366411475 1107 72-73 -2.6861495297670963 1107 74-75 -4.829846147028139 1107 76-77 -6.80361821123169 1107 78-79 -7.817607694249091 1107 80-81 -2.824083024763805 1107 82-83 -1.7584402459125847 1107 84-85 -1.6097478672842307 1107 86-87 -1.4536622635460326 1107 88-89 -1.316914592975177 1107 90-91 -1.0881569718596609 1107 92-93 -3.800325463771152 1107 94-95 -5.447852354001114 1107 96-97 -6.352684913282773 1107 98-99 -5.992337620291785 1107 100-101 -6.904784377189728 1108 1 -0.050536015005722135 1108 2 -0.06611707331224892 1108 3 -0.11100800082153484 1108 4 -0.1966862241803895 1108 5 -0.08266132218482625 1108 6 -0.2041187098072399 1108 7 -0.11474536755302012 1108 8 -0.10150721684178166 1108 9 -0.18603796742566203 1108 10-11 -0.08762797824721247 1108 12-13 -0.02231364445029982 1108 14-15 -0.20238107010304418 1108 16-17 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2.431744485481552 2316 92-93 4.536971569908349 2316 94-95 11.259531890648606 2316 96-97 13.172237629605517 2316 98-99 12.63541425876037 2316 100-101 11.47523025359804 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 22.0 5 70.0 6 163.0 7 365.0 8 760.0 9 1374.0 10 2269.0 11 3472.0 12 5164.0 13 7026.0 14 8544.0 15 11397.0 16 15107.0 17 20423.0 18 21236.0 19 19969.0 20 20189.0 21 19973.0 22 19911.0 23 17125.0 24 17299.0 25 20656.0 26 28358.0 27 42077.0 28 58190.0 29 77829.0 30 95748.0 31 104174.0 32 105063.0 33 95795.0 34 85553.0 35 75608.0 36 60379.0 37 37105.0 38 13000.0 39 793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.0458447928827 19.686891234861253 14.531398115987129 22.735865856268923 2 15.984249986062729 22.001823534383693 38.306001298414024 23.707925181139547 3 18.951459907174467 26.30764057349707 30.790581499270807 23.95031802005765 4 13.036101550446144 17.493589259447077 37.01721638991688 32.45309280018989 5 13.13454741912973 38.17635008891791 34.0839655133602 14.60513697859217 6 30.592359620066965 36.85879107639301 17.492690137349893 15.056159166190131 7 26.692507435739742 33.93745347043147 21.773500354254104 17.59653873957468 8 25.843098715402313 33.821235288987275 20.64248766158624 19.693178334024168 9 26.394448460523144 16.18042143650939 20.937856277131008 36.48727382583645 10-11 24.64471190329762 26.515470294804157 27.645172253811378 21.19464554808685 12-13 25.963049678294116 24.399071746346866 28.109388992587636 21.528489582771382 14-15 23.053009779795016 25.47305274004195 27.00705184631057 24.46688563385246 16-17 21.424730892756312 28.519163888379385 27.63923804796996 22.41686717089434 18-19 21.312224924586065 26.960694842181454 30.248832264126346 21.47824796910614 20-21 22.512758542559045 25.96794989372377 29.965401781700347 21.55388978201684 22-23 22.051173695201147 26.00075976056303 30.135745385803265 21.812321158432553 24-25 22.237277528995577 26.185279476494916 30.061756229008303 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21.881295242484413 26.99922049258388 28.850100543854946 22.269383721076764 52-53 22.18762514567519 27.211180655234635 28.223820087202633 22.377374111887548 54-55 21.904608079514713 27.004390639600878 28.249597445969577 22.841403834914832 56-57 21.16215234966481 27.087032342394522 28.918666335705673 22.832148972234993 58-59 20.660339170139725 26.6847713356549 30.140253313072186 22.514636181133195 60-61 21.758626082130263 27.139695932356293 28.914583911478875 22.18709407403457 62-63 21.753911778404788 27.08199583870579 28.675570109894856 22.48852227299457 64-65 21.34179303937715 27.485102416246527 28.566046517280476 22.607058027095846 66-67 21.1733066121976 27.13215960805741 28.779204086646885 22.915329693098098 68-69 21.267007294876457 26.738372994835014 29.2333550180925 22.761264692196033 70-71 21.31989229032024 26.724805098862625 29.150030739169473 22.805271871647662 72-73 20.469051938502922 26.801331623849517 29.67190657941895 23.057709858228613 74-75 20.87303481125928 27.4858891876761 28.750539193641643 22.890536807422976 76-77 19.25733338806862 27.51150581334355 29.624607160287724 23.606553638300102 78-79 20.190212753595823 27.38385520118814 29.072058070583793 23.353873974632247 80-81 21.546266733957843 27.395651442487186 27.75666681960117 23.301415003953807 82-83 21.876639493686138 27.0686223779902 27.710326107235645 23.34441202108802 84-85 21.521091175558244 27.323266153866903 27.590710262139282 23.56493240843557 86-87 21.494801493803468 27.243930805268324 27.661483670347227 23.59978403058098 88-89 21.48640435032018 27.10075760164144 27.282875109917875 24.129962938120507 90-91 20.91648612263533 27.084579338791947 27.853027618617094 24.14590691995563 92-93 20.018009438028802 27.300367184908183 28.60968492510737 24.07193845195565 94-95 18.951406590935687 27.260170566789267 29.1679763950615 24.62044644721355 96-97 18.925772967790707 27.312911373734767 28.74085067940043 25.0204649790741 98-99 19.2894595796604 27.267245213305813 28.376731933721818 25.06656327331197 100-101 19.64252805005479 26.920154369489964 28.3554983009733 25.081819279481948 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 977.0 1 743.5 2 505.0 3 551.0 4 639.5 5 733.0 6 931.5 7 1208.5 8 1506.5 9 1769.0 10 1903.5 11 1960.0 12 2072.0 13 2269.5 14 2419.5 15 2542.5 16 2648.0 17 2783.0 18 3006.0 19 3204.0 20 3413.0 21 3708.5 22 4145.5 23 4678.0 24 5233.5 25 5972.5 26 6929.5 27 8030.5 28 9246.0 29 10735.0 30 12539.0 31 14630.0 32 16783.0 33 19032.0 34 21269.5 35 23378.5 36 25767.5 37 27948.0 38 29647.5 39 30970.0 40 31975.0 41 32903.0 42 33898.0 43 35438.5 44 37307.5 45 39196.5 46 41316.5 47 43250.5 48 44815.5 49 44810.5 50 43475.5 51 41855.5 52 40000.0 53 38506.0 54 36608.0 55 34282.0 56 31526.5 57 27994.0 58 24590.5 59 21423.0 60 17819.5 61 14284.0 62 11058.5 63 7976.0 64 5634.5 65 4017.0 66 2848.5 67 1963.0 68 1275.0 69 814.5 70 522.0 71 327.0 72 208.0 73 144.5 74 90.0 75 46.0 76 25.0 77 14.0 78 8.0 79 3.5 80 1.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02823243385158731 2 0.006293854680290165 3 1.7982441943686185E-4 4 0.0 5 0.14574769195357654 6 0.0 7 0.0 8 0.0017083319846501876 9 0.0 10-11 0.0 12-13 0.0 14-15 4.495610485921546E-4 16-17 0.0 18-19 8.991220971843092E-5 20-21 0.0 22-23 3.1469273401450823E-4 24-25 4.495610485921546E-5 26-27 3.1469273401450823E-4 28-29 0.0028771907109897896 30-31 0.012497797150861899 32-33 0.002922146815849005 34-35 0.0037313567033148837 36-37 0.11032228132451474 38-39 0.11027732521965554 40-41 0.12268521016079899 42-43 0.12439354214544918 44-45 0.12048236102269745 46-47 0.11310955982578609 48-49 0.11279486709177158 50-51 0.1113113156314175 52-53 0.08910299983096505 54-55 0.07682998320439922 56-57 0.5687396825739348 58-59 1.6021456649727206 60-61 0.5330894914205769 62-63 0.0037763128081740992 64-65 0.1224154735316437 66-67 1.9262791810076643 68-69 2.9067268718822943 70-71 2.816724749954145 72-73 2.6621207053433027 74-75 2.0325554128948493 76-77 1.4395843898017975 78-79 0.9566659114041051 80-81 0.054756535718524436 82-83 4.495610485921546E-5 84-85 8.991220971843092E-5 86-87 1.3486831457764639E-4 88-89 1.7982441943686185E-4 90-91 0.25332765088167913 92-93 0.39952490388384776 94-95 2.0314315102733693 96-97 2.4650331416405025 98-99 1.4438552197634231 100-101 0.9605321364219975 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1112196.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.839013245752206 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64900121599 34.27617518216661 2 14.040996714227019 13.43414855590939 3 5.529396430492066 7.935626072379748 4 2.6757484322950993 5.120206587794638 5 1.5730470109915355 3.7626508397507488 6 1.0215081451472998 2.9320765011807266 7 0.6748417310552806 2.259863375751794 8 0.4904393814593017 1.8769708852696045 9 0.36929838998176345 1.590018351297529 >10 1.8231373988585788 15.261113928464898 >50 0.09786288445786889 3.197400756241749 >100 0.048912710558175525 4.223180354357163 >500 0.0033732897015627266 1.076011643488475 >1k 0.002061454817621666 1.874996459916511 >5k 3.7480996684030295E-4 1.1795605060304837 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7377 0.6632823710928649 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5780 0.5196925721725307 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4038 0.3630655028430241 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3759 0.3379799963315818 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2208 0.1985261590582955 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1846 0.1659779391402235 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1702 0.15303058094076943 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1688 0.1517718100047114 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1380 0.12407884941143467 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1184 0.10645605630662221 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.991220971843092E-5 0.0 0.0 0.0 0.0 3 8.991220971843092E-5 0.0 0.0 0.0 0.0 4 8.991220971843092E-5 0.0 0.0 0.0 0.0 5 8.991220971843092E-5 0.0 0.0 0.0 0.0 6 8.991220971843092E-5 0.0 0.0 0.0 0.0 7 8.991220971843092E-5 0.0 0.0 0.0 8.991220971843092E-5 8 8.991220971843092E-5 0.0 0.0 0.0 8.991220971843092E-5 9 8.991220971843092E-5 0.0 0.0 0.0 8.991220971843092E-5 10-11 1.7982441943686185E-4 0.0 0.0 0.0 8.991220971843092E-5 12-13 1.7982441943686185E-4 0.0 0.0 4.495610485921546E-5 8.991220971843092E-5 14-15 2.6973662915529277E-4 0.0 0.0 2.6973662915529277E-4 8.991220971843092E-5 16-17 2.6973662915529277E-4 0.0 0.0 4.0460494373293916E-4 8.991220971843092E-5 18-19 2.6973662915529277E-4 0.0 0.0 5.394732583105855E-4 8.991220971843092E-5 20-21 2.6973662915529277E-4 0.0 0.0 8.541659923250938E-4 8.991220971843092E-5 22-23 2.6973662915529277E-4 0.0 0.0 0.0013037270409172485 8.991220971843092E-5 24-25 5.394732583105855E-4 0.0 0.0 0.0021129369283831266 8.991220971843092E-5 26-27 5.394732583105855E-4 0.0 0.0 0.003416663969300375 8.991220971843092E-5 28-29 5.394732583105855E-4 0.0 0.0 0.009530694230153678 8.991220971843092E-5 30-31 5.394732583105855E-4 0.0 0.0 0.024950638196864584 8.991220971843092E-5 32-33 5.84429363169801E-4 0.0 0.0 0.058892497365572255 8.991220971843092E-5 34-35 6.293854680290165E-4 0.0 0.0 0.11104157900226219 8.991220971843092E-5 36-37 8.092098874658783E-4 0.0 0.0 0.18094832205834224 8.991220971843092E-5 38-39 8.092098874658783E-4 0.0 0.0 0.28326841671791664 1.3486831457764639E-4 40-41 8.092098874658783E-4 0.0 0.0 0.3939503468813051 1.7982441943686185E-4 42-43 8.092098874658783E-4 0.0 0.0 0.5077342482799794 1.7982441943686185E-4 44-45 8.092098874658783E-4 0.0 0.0 0.6458394024074894 1.7982441943686185E-4 46-47 9.440782020435247E-4 0.0 0.0 0.8000837981794575 1.7982441943686185E-4 48-49 0.001078946516621171 0.0 0.0 0.9365705325320357 2.6973662915529277E-4 50-51 0.001078946516621171 4.495610485921546E-5 0.0 1.0788116483065933 2.6973662915529277E-4 52-53 0.001078946516621171 8.991220971843092E-5 0.0 1.207880625357401 2.6973662915529277E-4 54-55 0.001078946516621171 8.991220971843092E-5 0.0 1.3534035367866815 2.6973662915529277E-4 56-57 0.001078946516621171 8.991220971843092E-5 0.0 1.477212649568961 2.6973662915529277E-4 58-59 0.001078946516621171 8.991220971843092E-5 0.0 1.5548068865559668 3.596488388737237E-4 60-61 0.001078946516621171 8.991220971843092E-5 4.495610485921546E-5 1.6152278914867524 3.596488388737237E-4 62-63 0.001078946516621171 8.991220971843092E-5 8.991220971843092E-5 1.6749745548446495 3.596488388737237E-4 64-65 0.001078946516621171 8.991220971843092E-5 8.991220971843092E-5 1.7332826228470521 3.596488388737237E-4 66-67 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 1.7823297332484562 3.596488388737237E-4 68-69 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 1.8495391100129832 3.596488388737237E-4 70-71 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 1.9569842006265084 3.596488388737237E-4 72-73 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 2.102776848684944 3.596488388737237E-4 74-75 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 2.2908731914159013 3.596488388737237E-4 76-77 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 2.5953608896273677 3.596488388737237E-4 78-79 0.001078946516621171 8.991220971843092E-5 1.7982441943686185E-4 3.0602969260813744 3.596488388737237E-4 80-81 0.001258770936058033 8.991220971843092E-5 1.7982441943686185E-4 3.609031141992958 3.596488388737237E-4 82-83 0.001258770936058033 8.991220971843092E-5 1.7982441943686185E-4 4.1479649270452335 4.495610485921546E-4 84-85 0.001258770936058033 8.991220971843092E-5 1.7982441943686185E-4 4.566191570550515 4.495610485921546E-4 86-87 0.001258770936058033 8.991220971843092E-5 1.7982441943686185E-4 4.886908422616157 4.495610485921546E-4 88-89 0.001258770936058033 8.991220971843092E-5 1.7982441943686185E-4 5.188923535060367 4.495610485921546E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5245 0.0 28.909542 1 TACATGG 5370 0.0 27.967226 2 ACATGGG 5255 0.0 26.696665 3 CATGGGA 3130 0.0 23.914772 4 GGTATCA 7790 0.0 23.393991 1 GTATCAA 10565 0.0 23.132822 1 CATGGGG 2250 0.0 22.178736 4 GAGTACT 5630 0.0 22.043661 12-13 AGTACTT 5755 0.0 20.788858 12-13 AGAGTAC 8420 0.0 20.657503 10-11 CGTATAG 115 0.0039148275 20.474012 1 GTACTTT 5965 0.0 20.372217 14-15 GTACCGG 140 5.1434455E-4 20.181524 1 TCAACGC 12210 0.0 19.93372 4 AACGCAG 12250 0.0 19.840937 6 ATCAACG 12285 0.0 19.812023 3 CAACGCA 12340 0.0 19.68557 5 ATGGGGC 1035 0.0 19.55877 5 TATCAAC 12630 0.0 19.271708 2 GTGTACG 125 0.00634044 18.836092 1 >>END_MODULE