FastQCFastQC Report
Fri 27 May 2016
SRR939664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068475
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28890.2703853623154496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.18559161421652356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.1338356068228082No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13870.12981117948477972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG40450.032.1897471
GTATCAA80200.030.0417961
GTATTAG2205.6341756E-527.9615291
TACCGTA700.00866348426.9657927
GGTATCA60250.025.1847151
GTATAGT3301.2493674E-624.8546921
TACATGG48600.024.5856482
ACATGGG48500.023.8573253
TATCAAC102650.023.6943992
GTATAGG3152.450075E-522.7834681
GAGTACT48750.022.74913212-13
GTTATAC2300.00239060122.2881761
CATGGGA30600.022.0694564
GGTCTAG2802.896216E-421.9697741
CTACTAG2350.00270857221.8139611
CAACGCA111900.021.7605975
TCAACGC112000.021.7574864
ATCAACG111200.021.6602173
AACGCAG114600.021.330276
ATGGGAG13600.021.1661645
Produced by FastQC (version 0.11.5)