##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939664_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1068475 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.047502281288752 2.0 2.0 31.0 2.0 33.0 2 22.47330821965886 16.0 16.0 31.0 16.0 34.0 3 27.03941411825265 28.0 25.0 31.0 16.0 34.0 4 32.34549708696974 35.0 32.0 35.0 25.0 37.0 5 32.96461218091205 35.0 33.0 35.0 28.0 37.0 6 33.383171342333696 35.0 33.0 37.0 28.0 37.0 7 33.558251713891295 35.0 33.0 37.0 28.0 37.0 8 33.494196869369894 35.0 33.0 37.0 28.0 37.0 9 34.85671634806617 37.0 34.0 39.0 28.0 39.0 10-11 34.71228433047099 37.0 34.0 39.0 27.0 39.0 12-13 34.72159105266853 37.0 34.0 39.0 27.0 39.0 14-15 35.367330073235216 38.0 34.0 40.0 27.0 41.0 16-17 34.98659912492103 38.0 33.0 40.0 26.0 41.0 18-19 35.26561407613654 38.0 34.0 40.0 27.0 41.0 20-21 35.26156344322516 38.0 34.0 40.0 26.5 41.0 22-23 35.06109455064461 38.0 34.0 40.0 25.5 41.0 24-25 34.84125318795479 38.0 33.0 40.0 25.0 41.0 26-27 34.90511429841597 38.0 34.0 40.0 25.0 41.0 28-29 34.63288893048504 38.0 33.0 40.0 24.0 41.0 30-31 34.46700765109151 38.0 33.0 40.0 24.0 41.0 32-33 34.09970799503966 38.0 33.0 40.0 23.0 41.0 34-35 33.78689206579471 38.0 32.5 40.0 21.0 41.0 36-37 33.46743302370201 37.0 32.0 40.0 19.0 41.0 38-39 33.22947939820773 37.0 31.0 40.0 18.0 41.0 40-41 32.80984300053815 37.0 31.0 40.0 15.5 41.0 42-43 32.49805096048106 37.0 31.0 40.0 15.0 41.0 44-45 32.07997192259997 36.5 30.0 39.5 11.0 41.0 46-47 31.637196471606728 36.0 30.0 39.0 8.5 40.5 48-49 31.196302674372355 36.0 29.5 39.0 7.5 40.0 50-51 30.471974075200635 35.5 28.0 38.5 4.0 40.0 52-53 30.481030908537868 35.0 28.0 38.5 2.0 40.0 54-55 30.59591754603524 35.0 28.0 39.0 2.0 40.0 56-57 30.27410608577646 35.0 28.0 39.0 2.0 40.0 58-59 29.850533704578957 35.0 27.5 38.0 2.0 40.0 60-61 29.25446454058354 34.0 26.0 38.0 2.0 40.0 62-63 28.854912843070732 34.0 26.0 38.0 2.0 40.0 64-65 28.278921359882077 34.0 25.5 37.0 2.0 39.5 66-67 27.67559465593486 33.0 24.5 36.5 2.0 39.0 68-69 27.335853435971828 33.0 23.5 36.0 2.0 39.0 70-71 26.800899412714383 32.5 22.5 36.0 2.0 38.5 72-73 26.201453473408364 32.0 21.5 35.0 2.0 38.0 74-75 25.656024708112028 31.5 20.0 35.0 2.0 37.0 76-77 25.040001871826668 31.0 18.0 35.0 2.0 36.5 78-79 24.398261073024635 31.0 14.0 34.0 2.0 36.0 80-81 23.664541519455298 30.0 7.5 34.0 2.0 35.5 82-83 22.98234259107607 29.5 4.5 34.0 2.0 35.0 84-85 22.30543297690634 29.0 2.0 34.0 2.0 35.0 86-87 21.642795105173263 29.0 2.0 34.0 2.0 35.0 88-89 20.853614263319216 27.5 2.0 33.0 2.0 35.0 90-91 19.9476571749456 26.5 2.0 32.5 2.0 34.0 92-93 18.941607431151873 25.0 2.0 32.0 2.0 34.0 94-95 17.970836472542643 23.5 2.0 32.0 2.0 34.0 96-97 17.014017641966355 19.5 2.0 31.5 2.0 34.0 98-99 15.95393060202625 9.5 2.0 31.0 2.0 34.0 100-101 14.381684176045297 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.776719038961467 1101 2 8.075143545988631 1101 3 3.678817051502591 1101 4 2.387347219433977 1101 5 1.7314176832048176 1101 6 1.3793102123045458 1101 7 1.085451674839696 1101 8 0.938876153738093 1101 9 1.2287798871666311 1101 10-11 1.210519652188431 1101 12-13 0.8435887760093905 1101 14-15 0.8854594812835543 1101 16-17 0.9124800475398871 1101 18-19 0.9199212266502883 1101 20-21 0.9943351013513535 1101 22-23 0.8987477384619993 1101 24-25 1.0712956567233576 1101 26-27 1.0142218939064307 1101 28-29 1.0929670214880005 1101 30-31 1.1438853720908426 1101 32-33 0.9021328363567065 1101 34-35 1.025910741454794 1101 36-37 1.2912690428680662 1101 38-39 1.2798866103684432 1101 40-41 1.4245906704231786 1101 42-43 1.0742035666821792 1101 44-45 0.8766514305869677 1101 46-47 0.9338246040909297 1101 48-49 0.5338353669641016 1101 50-51 0.7024220285934106 1101 52-53 0.7280228015048529 1101 54-55 0.7320521997230962 1101 56-57 0.4367178318541427 1101 58-59 0.6406256731143039 1101 60-61 0.6478867714137948 1101 62-63 0.29239501367352005 1101 64-65 0.3129298970419221 1101 66-67 0.31234875154360253 1101 68-69 0.10895268074942521 1101 70-71 -0.25114021399251385 1101 72-73 -0.18245925117897954 1101 74-75 0.15657088091610305 1101 76-77 0.2967413311191649 1101 78-79 0.3637903641422149 1101 80-81 -0.048110897140183795 1101 82-83 -0.28294558580111584 1101 84-85 -0.48991769019050224 1101 86-87 -0.40174076289468985 1101 88-89 -0.5410251077981023 1101 90-91 -0.4325708932442396 1101 92-93 -0.24207775289239564 1101 94-95 -0.17309696664324647 1101 96-97 -0.9414656948310238 1101 98-99 -1.2749193006584196 1101 100-101 -0.9652134533586629 1102 1 15.049611625740532 1102 2 8.117333887323898 1102 3 3.966855864714013 1102 4 2.457132700336544 1102 5 1.5936402957056828 1102 6 1.2814605256861071 1102 7 1.1096191921918503 1102 8 0.9583991112696779 1102 9 1.1068036624539417 1102 10-11 1.2013351760094508 1102 12-13 0.9335005608390148 1102 14-15 1.026936344467721 1102 16-17 1.3518091585461036 1102 18-19 1.1428026538993024 1102 20-21 0.9559840042414933 1102 22-23 0.9051620899835342 1102 24-25 0.734360173442532 1102 26-27 0.9930524483338345 1102 28-29 1.0440560818555724 1102 30-31 0.7595178613246318 1102 32-33 0.6332540215353859 1102 34-35 0.9026162286506079 1102 36-37 0.8115746310484724 1102 38-39 0.7442397783943804 1102 40-41 0.6826085183535398 1102 42-43 0.7617078201096206 1102 44-45 0.8823098611545603 1102 46-47 0.5477355986062946 1102 48-49 0.43784694360445897 1102 50-51 0.1917271740514792 1102 52-53 0.6466822675304833 1102 54-55 0.7679741781431275 1102 56-57 0.5832816113125574 1102 58-59 0.5573913120065477 1102 60-61 0.7651818476827827 1102 62-63 0.24832145590557175 1102 64-65 0.4380553045477136 1102 66-67 0.464651456540512 1102 68-69 0.23761035393252428 1102 70-71 0.19005857565583284 1102 72-73 0.2428520265323293 1102 74-75 -0.08645002216429276 1102 76-77 0.11853973512342719 1102 78-79 -0.2655972520723999 1102 80-81 -0.3708210896329298 1102 82-83 -0.3078258495319126 1102 84-85 -0.3476223395068416 1102 86-87 -0.35087891787677705 1102 88-89 -0.3473084720488231 1102 90-91 -0.010365623750736574 1102 92-93 -0.2354471626081427 1102 94-95 -0.27079224927206624 1102 96-97 -0.9281565831662046 1102 98-99 -1.1233959333342547 1102 100-101 -1.2573242144386132 1103 1 14.855251141098716 1103 2 7.877043085012634 1103 3 3.554763930882949 1103 4 2.08976120189088 1103 5 1.5984109347474202 1103 6 1.2004500802255293 1103 7 0.9194179470033959 1103 8 0.7909079162848798 1103 9 0.7404805851334189 1103 10-11 0.7847212533213579 1103 12-13 0.3225692098181483 1103 14-15 0.6915836702403695 1103 16-17 0.8960301407466247 1103 18-19 0.9073599919860484 1103 20-21 0.8397502687622307 1103 22-23 0.7426611212165852 1103 24-25 1.185596750638183 1103 26-27 1.060504462077411 1103 28-29 1.1714004978828498 1103 30-31 0.8383980958954069 1103 32-33 0.8927597781639065 1103 34-35 1.0532820944699282 1103 36-37 1.065924394669338 1103 38-39 0.806536875538022 1103 40-41 0.6853296770849298 1103 42-43 0.6154153988811402 1103 44-45 0.67706797081037 1103 46-47 0.6770595284417311 1103 48-49 0.5472853171093313 1103 50-51 0.2555011025952325 1103 52-53 0.5428390613854894 1103 54-55 0.3770584135061341 1103 56-57 0.4699821632944392 1103 58-59 0.3271703039722418 1103 60-61 0.24021672329310562 1103 62-63 0.19479815813675216 1103 64-65 0.1242996749353047 1103 66-67 0.5753406158230199 1103 68-69 0.07292104825856427 1103 70-71 0.001080129644140726 1103 72-73 -0.007640968206192866 1103 74-75 0.4015770946991424 1103 76-77 -0.03598182317051268 1103 78-79 -0.15649041214714643 1103 80-81 -0.2034558343520736 1103 82-83 -0.2959132452280038 1103 84-85 -0.26727603761322527 1103 86-87 -0.3380621310830101 1103 88-89 -0.39342608466259676 1103 90-91 -0.1578259473619532 1103 92-93 -0.18358799118375302 1103 94-95 -0.21820650263260788 1103 96-97 -1.0074835269432754 1103 98-99 -1.1699688664161396 1103 100-101 -1.2481844364369419 1104 1 14.822900154126582 1104 2 8.091131653181137 1104 3 3.788236598160779 1104 4 2.2290313667647084 1104 5 1.6075559513861961 1104 6 1.2573288877288178 1104 7 0.9718532071821997 1104 8 1.1000351306993537 1104 9 1.2064222667287225 1104 10-11 0.945756740022965 1104 12-13 0.7506240434724774 1104 14-15 0.9712510069435041 1104 16-17 1.144785386795796 1104 18-19 0.8118692813829327 1104 20-21 0.893430633329352 1104 22-23 1.1482133125722669 1104 24-25 1.1918086055178208 1104 26-27 1.082712386450197 1104 28-29 0.9679242281076625 1104 30-31 0.7799104298931709 1104 32-33 0.9422853558951445 1104 34-35 0.9496742003749361 1104 36-37 1.180888565940755 1104 38-39 0.9083946848271367 1104 40-41 1.0448631186333586 1104 42-43 1.057751611408861 1104 44-45 0.7385315519450657 1104 46-47 0.7390112689946449 1104 48-49 0.7799428667943609 1104 50-51 0.624609547577947 1104 52-53 0.8253533522261733 1104 54-55 0.8412230266091321 1104 56-57 0.804442153145768 1104 58-59 0.8095660768551056 1104 60-61 0.789553765656791 1104 62-63 0.6871558174386259 1104 64-65 0.9879710823202892 1104 66-67 0.6001754195920448 1104 68-69 0.49310715859871124 1104 70-71 0.2579087544994074 1104 72-73 0.16808661010256998 1104 74-75 0.04017967616183782 1104 76-77 0.1681925180770989 1104 78-79 0.1190172315953042 1104 80-81 0.33371612111713134 1104 82-83 0.09271596948364191 1104 84-85 -0.23504174632397934 1104 86-87 -0.4937688653700931 1104 88-89 -0.37509399888671524 1104 90-91 -0.12710834951279892 1104 92-93 0.18756385478075188 1104 94-95 0.5378234751917041 1104 96-97 -0.28326240930001134 1104 98-99 -0.5587943261200348 1104 100-101 -0.21555204816342055 1105 1 14.76778249490524 1105 2 7.81247684091619 1105 3 3.552608709767995 1105 4 2.161589665497864 1105 5 1.57175454986956 1105 6 1.252737495767981 1105 7 0.8186112220355994 1105 8 0.7259264461020649 1105 9 0.7315998312862249 1105 10-11 0.621815631103658 1105 12-13 0.6258975152437714 1105 14-15 0.6634810557283544 1105 16-17 1.066527056209985 1105 18-19 1.001756134620443 1105 20-21 0.8824309909731554 1105 22-23 0.8119869296363476 1105 24-25 1.0712344475174973 1105 26-27 1.033993547563142 1105 28-29 1.3073065381513018 1105 30-31 1.1127608509628644 1105 32-33 0.564186009153893 1105 34-35 0.896258841809086 1105 36-37 0.6930725541161564 1105 38-39 0.7947806104260522 1105 40-41 1.032044871535021 1105 42-43 0.9915113697757647 1105 44-45 1.1425604232994644 1105 46-47 0.7895476648392687 1105 48-49 0.6080227247427104 1105 50-51 0.6032487129267317 1105 52-53 0.9335978799966576 1105 54-55 0.7268807419358474 1105 56-57 0.7986957533335328 1105 58-59 0.6750619723337046 1105 60-61 0.7107907521725032 1105 62-63 0.6526767200421588 1105 64-65 0.8687548531153162 1105 66-67 0.7767026703729947 1105 68-69 0.49897125954367283 1105 70-71 0.42327325779364244 1105 72-73 0.552548453979874 1105 74-75 0.22245647010872105 1105 76-77 0.3713719155590667 1105 78-79 0.48545194204353237 1105 80-81 0.36269504807397723 1105 82-83 0.3269555433287046 1105 84-85 0.2785005199082988 1105 86-87 0.12257189372886756 1105 88-89 0.1936329847790219 1105 90-91 0.26648144422731335 1105 92-93 0.6219110710267195 1105 94-95 0.4721308756242344 1105 96-97 0.002013004365334581 1105 98-99 -0.17651362044411556 1105 100-101 0.04884486101181906 1106 1 14.583285965041469 1106 2 7.840266538645295 1106 3 3.410163622599775 1106 4 2.1962631351191533 1106 5 1.491338505424352 1106 6 1.0001306024124474 1106 7 0.6168655690034441 1106 8 0.7725508589252641 1106 9 0.9064626564863403 1106 10-11 0.7618637352592401 1106 12-13 0.39897370661906706 1106 14-15 0.6829465746467491 1106 16-17 0.9276829863380911 1106 18-19 0.8593414433281126 1106 20-21 0.5233326828433675 1106 22-23 0.6685891999579638 1106 24-25 0.7641981956079178 1106 26-27 0.8025153639348588 1106 28-29 0.7226579573883924 1106 30-31 0.7266960369427053 1106 32-33 0.7760502704697458 1106 34-35 0.6553869021373941 1106 36-37 0.8699719030007316 1106 38-39 0.8635918670559235 1106 40-41 0.9051149122154314 1106 42-43 0.44286724039947956 1106 44-45 0.3827361186140692 1106 46-47 0.908775666807486 1106 48-49 0.6761248634680577 1106 50-51 0.5237817350062954 1106 52-53 0.5874324806592135 1106 54-55 0.523728653846451 1106 56-57 0.63741630866976 1106 58-59 0.4981415251352779 1106 60-61 0.6594699977254095 1106 62-63 0.42692333143839534 1106 64-65 0.6989248697651043 1106 66-67 0.4620939117834766 1106 68-69 0.6655728432760881 1106 70-71 0.5276544835842216 1106 72-73 0.7846252575638708 1106 74-75 0.6253999352099591 1106 76-77 0.42374007612939124 1106 78-79 0.32562184426178575 1106 80-81 0.5588464295486553 1106 82-83 0.44068224935981704 1106 84-85 0.5343043442101241 1106 86-87 0.34264196873873587 1106 88-89 0.4757946822563781 1106 90-91 0.8975627649960671 1106 92-93 1.3881675006484677 1106 94-95 1.1115606919044083 1106 96-97 0.839106843943771 1106 98-99 0.3740465219087099 1106 100-101 0.16467067047187278 1107 1 14.690871171186831 1107 2 7.922898088952746 1107 3 3.5408059109674817 1107 4 1.8818773993262212 1107 5 1.4648082411447305 1107 6 1.171248848045579 1107 7 0.8385883746844627 1107 8 0.9677063975514457 1107 9 0.6701861514347343 1107 10-11 0.9209734920204014 1107 12-13 0.6484467798446545 1107 14-15 0.7836002903420862 1107 16-17 1.1519832892529678 1107 18-19 0.9453892204408092 1107 20-21 0.7184991046387239 1107 22-23 0.6213264983925981 1107 24-25 0.8320133993952652 1107 26-27 0.7739753410802095 1107 28-29 0.9436345404360438 1107 30-31 0.9483927230176974 1107 32-33 0.7625638472232978 1107 34-35 0.8549272696257404 1107 36-37 0.8016078247144662 1107 38-39 0.6794464679157031 1107 40-41 1.1072023040639394 1107 42-43 1.0411636616506712 1107 44-45 1.2440315785186655 1107 46-47 0.8920348132042548 1107 48-49 1.115862579090848 1107 50-51 0.8654591941490679 1107 52-53 0.8310946670878998 1107 54-55 0.9821973919429858 1107 56-57 0.900461782178315 1107 58-59 0.996227901221328 1107 60-61 1.2625539250983948 1107 62-63 0.8809074782886235 1107 64-65 0.9849214959826611 1107 66-67 0.9580413823535743 1107 68-69 0.9425861388811008 1107 70-71 0.8709089139410082 1107 72-73 0.84167918366715 1107 74-75 0.7994820305828014 1107 76-77 0.5025599778180982 1107 78-79 0.6159481579509993 1107 80-81 0.2674858520151453 1107 82-83 0.5221772340326858 1107 84-85 0.7733934229498516 1107 86-87 0.8427921564734078 1107 88-89 1.096346821706053 1107 90-91 1.2039682356024421 1107 92-93 1.4204516964729557 1107 94-95 1.4324386008848364 1107 96-97 1.076950888129435 1107 98-99 0.4244816672897116 1107 100-101 0.14484407563412383 1108 1 14.455983232656497 1108 2 7.632757723754796 1108 3 3.4831919418797668 1108 4 -0.01911811111687456 1108 5 0.27845294506466445 1108 6 0.609664549073031 1108 7 0.41154101252095643 1108 8 0.41975757495104915 1108 9 0.7895955883768622 1108 10-11 0.6725405780672915 1108 12-13 0.38371198311688204 1108 14-15 0.50403540471045 1108 16-17 0.8294237094612953 1108 18-19 0.5152044920323817 1108 20-21 0.4550817178092714 1108 22-23 -0.622851806222954 1108 24-25 0.2161391779696089 1108 26-27 0.12115685759219019 1108 28-29 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-1.345554336801925 2312 5 -1.4914026320018934 2312 6 0.004093322089531171 2312 7 0.19532362653345814 2312 8 0.3811250242120323 2312 9 0.2826485581086402 2312 10-11 0.37187713392864197 2312 12-13 -0.5014112593435414 2312 14-15 0.2642862590055586 2312 16-17 0.28977816464291806 2312 18-19 0.6401699698536873 2312 20-21 0.6848410724609408 2312 22-23 0.8436795426633381 2312 24-25 0.660819877014525 2312 26-27 0.44752541293691195 2312 28-29 0.15760313379718127 2312 30-31 0.599528760032662 2312 32-33 0.9499759542021664 2312 34-35 0.04474324635117455 2312 36-37 0.8131599295902134 2312 38-39 0.7205916184642618 2312 40-41 0.804439501301836 2312 42-43 0.8962980862712371 2312 44-45 0.9016441832366695 2312 46-47 0.8828322473940275 2312 48-49 0.9462502547892591 2312 50-51 1.0550056205923326 2312 52-53 0.9584892480406175 2312 54-55 0.9025307369603333 2312 56-57 1.0365990460151089 2312 58-59 0.9158973603681595 2312 60-61 1.1071916621378932 2312 62-63 1.037996755865823 2312 64-65 0.66057539201827 2312 66-67 0.6920236460151123 2312 68-69 1.0154968613038982 2312 70-71 0.9808184796460822 2312 72-73 1.0101495121048814 2312 74-75 1.22729653512204 2312 76-77 1.1192296823292978 2312 78-79 1.3247858318968078 2312 80-81 1.0636669309173108 2312 82-83 0.9437798138637383 2312 84-85 1.0411479884796648 2312 86-87 1.3303830289189875 2312 88-89 1.4632738082811159 2312 90-91 1.8032808648568839 2312 92-93 2.4214422674708906 2312 94-95 2.6133289230131957 2312 96-97 2.1227299199917518 2312 98-99 1.858483560100538 2312 100-101 1.7599564174617388 2313 1 -14.210405424557848 2313 2 -7.29158626753193 2313 3 -3.2219474892199926 2313 4 -0.8162460862859859 2313 5 -0.26643032909633746 2313 6 -0.9716407365036872 2313 7 -0.3026153535835192 2313 8 0.1462968009755059 2313 9 -1.9384030139496815 2313 10-11 -0.147687833389476 2313 12-13 -0.5112815588829918 2313 14-15 -0.7231035175141542 2313 16-17 -0.5695798242398951 2313 18-19 0.07762246711136811 2313 20-21 -0.7798192837873685 2313 22-23 -1.7973335889776223 2313 24-25 -1.1125740681061984 2313 26-27 -0.3134596176223283 2313 28-29 0.26356591766672466 2313 30-31 -0.9911725482014191 2313 32-33 -0.17881108424236203 2313 34-35 -1.0774485284676771 2313 36-37 -1.1748467834459788 2313 38-39 -0.41867135808666944 2313 40-41 0.07077599395268663 2313 42-43 -0.05964778491022571 2313 44-45 -0.03594012343213393 2313 46-47 -0.5717376704801538 2313 48-49 -0.05340361712075037 2313 50-51 -1.0838116004955296 2313 52-53 0.0234640981202503 2313 54-55 -0.9392709230850471 2313 56-57 -0.4912815545154139 2313 58-59 -0.44136605714517074 2313 60-61 -1.8215323132065535 2313 62-63 -0.30419006154972195 2313 64-65 -0.02784941362275717 2313 66-67 -1.1795734458242073 2313 68-69 -0.49248257231962 2313 70-71 -0.2422047557853837 2313 72-73 -1.2298589921698522 2313 74-75 -0.4629650432220487 2313 76-77 0.26763098823531806 2313 78-79 0.3950327160592728 2313 80-81 0.5249892566241883 2313 82-83 0.3233498344465495 2313 84-85 0.27116636647755854 2313 86-87 0.19895706335513808 2313 88-89 0.8242830096051001 2313 90-91 1.1397875525582783 2313 92-93 1.1915830878526634 2313 94-95 1.286933215412347 2313 96-97 0.8744362527321137 2313 98-99 0.7991665861981154 2313 100-101 0.9872444576577877 2314 1 -14.210405424557848 2314 2 -7.290418131514002 2314 3 -3.5261070845675064 2314 4 -1.1442691326067482 2314 5 -1.501309006094015 2314 6 -5.946234837487424 2314 7 -1.8622775122621569 2314 8 -4.223527717309636 2314 9 -1.3171990146022523 2314 10-11 -0.10227566288116208 2314 12-13 -2.9015685855813302 2314 14-15 -2.688730373625077 2314 16-17 -2.351326604273666 2314 18-19 -1.397487548842797 2314 20-21 -1.9663520314989498 2314 22-23 -1.0391995414852033 2314 24-25 -2.927504668537999 2314 26-27 -4.562454095460588 2314 28-29 -1.883293639755216 2314 30-31 -2.0175799084470825 2314 32-33 -4.178234431848992 2314 34-35 -2.6055138084848544 2314 36-37 -0.8404406577237467 2314 38-39 -0.9501223963081671 2314 40-41 -1.9802671006613792 2314 42-43 -1.0176284350972793 2314 44-45 -2.172451365858496 2314 46-47 -1.7083364877954033 2314 48-49 -0.4671345828245883 2314 50-51 -1.6627585972065262 2314 52-53 -0.4490109024270943 2314 54-55 0.09756173330852036 2314 56-57 -0.6882787952001657 2314 58-59 -0.9665968063836559 2314 60-61 0.16392813649049742 2314 62-63 -2.137339416991477 2314 64-65 -0.42079299632558786 2314 66-67 -1.7150712914113164 2314 68-69 -1.6296852326187476 2314 70-71 -2.1667822952276303 2314 72-73 -2.027544048856935 2314 74-75 -1.2190410808255763 2314 76-77 -0.22390073188381265 2314 78-79 0.2073268885860422 2314 80-81 0.42571358570519635 2314 82-83 0.09107580414422145 2314 84-85 0.5154932439878337 2314 86-87 0.7142247564842528 2314 88-89 1.1251553684982945 2314 90-91 1.491915233921283 2314 92-93 1.8940025560329623 2314 94-95 1.8924129315849534 2314 96-97 1.5030265583331648 2314 98-99 1.5915678402140259 2314 100-101 1.5405479025290418 2315 1 -14.210405424557848 2315 2 -7.430250556751998 2315 3 -4.541416876048711 2315 4 -2.7997252439095632 2315 5 -3.910891417558343 2315 6 -2.8168761973396528 2315 7 -3.172180632649791 2315 8 -3.463501938807571 2315 9 -2.5143458360752504 2315 10-11 -4.896393075403893 2315 12-13 -4.443445602698368 2315 14-15 -3.659104669355827 2315 16-17 -4.357322498271543 2315 18-19 -1.832470049038534 2315 20-21 -3.196087345147056 2315 22-23 -4.325637048190799 2315 24-25 -5.460069238031213 2315 26-27 -4.792819735530067 2315 28-29 -2.4331216864174934 2315 30-31 -3.036739801853411 2315 32-33 -4.5094247520069715 2315 34-35 -3.668828085831379 2315 36-37 -5.086586206220101 2315 38-39 -3.7510110152009943 2315 40-41 -4.720504834125009 2315 42-43 -3.42146650318848 2315 44-45 -1.8446624161077274 2315 46-47 -1.5245986919878192 2315 48-49 -1.2424723275971168 2315 50-51 -1.0261205749308857 2315 52-53 -0.6424323098927083 2315 54-55 -2.180068601812007 2315 56-57 -4.214152987990882 2315 58-59 -3.520310476529019 2315 60-61 -4.56711857508315 2315 62-63 -2.513533738761698 2315 64-65 -3.162869514111012 2315 66-67 -1.501531633404177 2315 68-69 -1.3071194017477552 2315 70-71 -1.9439001050118634 2315 72-73 -2.1774218257003604 2315 74-75 -2.758792241438112 2315 76-77 -2.4261376476266783 2315 78-79 -1.745894119298292 2315 80-81 -1.463615738283309 2315 82-83 -0.9212019686395969 2315 84-85 -0.8558107016598342 2315 86-87 -1.4995834879968584 2315 88-89 -1.88540359583806 2315 90-91 -1.1182334926671125 2315 92-93 -0.30655687225843664 2315 94-95 -0.03393058072870048 2315 96-97 -1.1706912584103932 2315 98-99 -0.5912953278449908 2315 100-101 -0.44548451342985196 2316 1 -14.210405424557848 2316 2 -7.360370237804416 2316 3 -5.026932955161765 2316 4 -2.8505207958445347 2316 5 -1.772210392395646 2316 6 -0.9949630872373234 2316 7 -4.834828151963201 2316 8 -2.831777829071676 2316 9 -1.144670840390674 2316 10-11 -3.8445614435079207 2316 12-13 -3.572641026230599 2316 14-15 -1.8646724729722948 2316 16-17 -3.2300061762834815 2316 18-19 -2.5119462021648715 2316 20-21 -3.2733599072361415 2316 22-23 -3.327174322842467 2316 24-25 -3.3331450895322234 2316 26-27 -3.972733866191973 2316 28-29 -3.7421975500319675 2316 30-31 -2.582845170844756 2316 32-33 -3.0247744072274187 2316 34-35 -4.062073995265045 2316 36-37 -2.190330478821558 2316 38-39 -3.154128264474945 2316 40-41 -2.619651516095022 2316 42-43 -6.332608167154852 2316 44-45 -3.3568466710235256 2316 46-47 -4.830424361029735 2316 48-49 -4.186707225889425 2316 50-51 -3.413958578510414 2316 52-53 -4.519018795503424 2316 54-55 -4.727928530766523 2316 56-57 -4.695595156785988 2316 58-59 -3.4080557363780244 2316 60-61 -2.9087137975318242 2316 62-63 -2.2313921686746916 2316 64-65 -2.3166552753364122 2316 66-67 -2.579148672206639 2316 68-69 -2.9078826258680905 2316 70-71 -2.880465286062769 2316 72-73 -3.402049412304077 2316 74-75 -3.9696032834688104 2316 76-77 -3.767127575278998 2316 78-79 -4.0888134950913475 2316 80-81 -3.912298550182367 2316 82-83 -3.37999384716575 2316 84-85 -2.9922106095974854 2316 86-87 -3.0757775729909973 2316 88-89 -2.9535944647067573 2316 90-91 -2.2689038216385846 2316 92-93 -1.9015222883105949 2316 94-95 -1.1215586427859883 2316 96-97 -1.1557780665421653 2316 98-99 -1.818952416797206 2316 100-101 -1.950845101054128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7038.0 3 2041.0 4 2397.0 5 2925.0 6 3494.0 7 4355.0 8 5595.0 9 6869.0 10 8120.0 11 9823.0 12 11691.0 13 13033.0 14 14252.0 15 15823.0 16 17845.0 17 18487.0 18 16643.0 19 15912.0 20 17281.0 21 18465.0 22 20515.0 23 22927.0 24 25236.0 25 28438.0 26 31715.0 27 36017.0 28 40394.0 29 45462.0 30 51217.0 31 58076.0 32 66403.0 33 76510.0 34 88421.0 35 100554.0 36 101016.0 37 56599.0 38 6858.0 39 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.96515714837163 18.713354056195527 13.838605473273603 22.482883322159243 2 15.873148196716397 21.34463211216102 39.272748049846015 23.509471641276576 3 19.193860303349435 26.246434875815623 30.277601837936295 24.28210298289865 4 12.772962207252487 16.773626093349137 37.584110992753516 32.86930070664486 5 13.367308885178375 37.74472048409071 34.037112621552076 14.850858009178832 6 30.439045055447668 36.3469959285891 18.06997878067617 15.14398023528706 7 26.79429066327736 33.651830939446086 22.15357995808354 17.400298439193016 8 25.996098930814604 33.04670503999018 20.974386228344706 19.98280980085051 9 27.033217497572696 16.11616564888644 21.024377991955227 35.82623886158564 10-11 24.69199835263922 26.215760219985444 27.767868312340727 21.324373115034604 12-13 26.27024250946904 24.315096897417646 27.987812906941272 21.426847686172046 14-15 23.532716937452843 25.268732045868852 26.965195345633138 24.23335567104517 16-17 21.66935483115172 28.37417750132846 27.559059176793752 22.397408490726068 18-19 21.567681914123863 26.785305905156093 30.236744234871576 21.41026794584847 20-21 22.84542910006108 25.739253454912113 29.993713867040555 21.421603577986254 22-23 22.382011535587125 25.76448348194318 30.146825087979717 21.70667989448998 24-25 22.409174430678235 25.958014776712563 30.131540317989153 21.501270474620053 26-27 21.895851944598803 25.874664997221497 30.65317042174593 21.576312636433766 28-29 21.79499924969988 26.510682397959183 30.307060324129655 21.387258028211285 30-31 21.926209133528562 26.140471665725535 30.030888458241016 21.902430742504883 32-33 21.726391040241907 26.23731522398223 30.0844737262935 21.95182000948237 34-35 22.39898493770974 26.153233705807306 29.89646345883601 21.55131789764695 36-37 22.319114541724332 25.848823602218896 30.133917687786692 21.69814416827008 38-39 21.885315262310336 26.45737471666358 30.01024347593721 21.647066545088876 40-41 22.021716840936165 26.371789203839292 30.02218941499738 21.584304540227166 42-43 21.878507117602883 26.504878673068433 29.26102190082141 22.355592308507276 44-45 22.02509888205607 26.27397971909807 29.33590079724537 22.36502060160049 46-47 22.578909923823076 26.588762320772247 28.928472104650766 21.903855650753915 48-49 22.28081604245019 26.71671842089342 28.72460419858803 22.277861338068362 50-51 21.93844525872016 27.050792793097045 28.846514656024798 22.164247292158 52-53 22.352640896036817 27.195366672906868 28.16724306434306 22.284749366713257 54-55 21.993300932615263 27.018211330243382 28.225075528700906 22.76341220844045 56-57 22.190059000647224 27.141610933410874 27.892673226463057 22.775656839478845 58-59 22.366393497572325 26.816747932948637 28.18770331220457 22.62915525727447 60-61 22.10791829003568 27.05969778377897 28.635104153480455 22.197279772704896 62-63 21.85847359361879 26.925332936976996 28.743961465912026 22.472232003492188 64-65 21.84888565512152 27.414070107222205 28.24110298554134 22.495941252114932 66-67 21.887315107813823 27.195040128057197 27.910532507313167 23.007112256815816 68-69 21.777152292112223 27.04906197464908 28.302872160585213 22.870913572653485 70-71 22.374750795672142 26.8685870325602 27.96968099970555 22.78698117206211 72-73 21.963847313638272 26.86113175869758 28.122794388337724 23.052226539326423 74-75 21.696588612069263 27.813640954481205 27.609298249811303 22.88047218363823 76-77 21.9080904084141 27.100616421005224 27.784637324901716 23.206655845678956 78-79 21.829775197358455 27.17380443863605 27.984253541991418 23.012166822014084 80-81 22.175566162759335 27.191809257977884 27.553918866618975 23.078705712643814 82-83 22.1231976817 26.90337805542174 27.83437551450415 23.13904874837411 84-85 21.824674274165375 27.1106588606571 27.693342930629832 23.37132393454769 86-87 21.925478276651734 27.018779331714104 27.703301284862153 23.352441106772012 88-89 21.910911487877296 26.924233165231477 27.367241664747716 23.797613682143513 90-91 21.79444614823339 26.820138972906005 27.556550943445774 23.82886393541483 92-93 21.960790198299858 26.926445983358715 27.55508954630373 23.557674272037694 94-95 21.860249767092327 26.63605326338128 27.665584924282726 23.838112045243665 96-97 21.69630753363368 26.442819114617034 27.81955804521841 24.041315306530876 98-99 21.803992905714427 26.675048774386607 27.31961690107001 24.201341418828957 100-101 21.885911394781303 26.312230148301108 27.677022714473438 24.124835742444155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2602.0 1 1881.5 2 1116.0 3 952.5 4 834.5 5 855.5 6 1118.0 7 2224.0 8 2907.5 9 2531.0 10 2126.5 11 1920.0 12 1936.5 13 1922.0 14 1983.5 15 2106.0 16 2199.0 17 2313.5 18 2498.5 19 2697.5 20 2934.5 21 3285.5 22 3788.5 23 4313.5 24 4772.5 25 5583.0 26 6702.0 27 7818.0 28 8747.5 29 10225.5 30 12232.5 31 14349.0 32 16248.0 33 17551.5 34 19139.5 35 21325.5 36 23993.0 37 26277.5 38 27943.5 39 28782.0 40 28934.5 41 29029.0 42 29348.5 43 30779.5 44 32803.5 45 34910.5 46 37367.5 47 41846.0 48 44659.0 49 42483.0 50 39614.5 51 37790.0 52 37222.0 53 36749.0 54 35642.0 55 33710.5 56 30937.0 57 28451.0 58 25920.5 59 23191.5 60 19956.0 61 16387.5 62 12988.5 63 9730.0 64 7084.0 65 5146.5 66 3671.0 67 2671.0 68 1899.5 69 1347.5 70 935.0 71 613.5 72 403.0 73 267.5 74 169.0 75 111.5 76 86.5 77 51.0 78 29.0 79 20.5 80 14.0 81 11.5 82 10.5 83 7.0 84 3.5 85 3.5 86 4.5 87 6.0 88 4.0 89 1.0 90 1.5 91 2.0 92 1.0 93 1.0 94 1.5 95 1.5 96 1.0 97 0.0 98 1.0 99 3.0 100 2.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 53.93340976625565 2 0.08114368609466764 3 0.11146727813004516 4 0.18970963288799456 5 0.11577247946840123 6 0.14253960083296285 7 0.14890381150705445 8 0.14862303750672687 9 0.13542665949133112 10-11 0.12293221647675426 12-13 0.1351926811577248 14-15 0.1422588268326353 16-17 0.13589461615854373 18-19 0.14441142750181332 20-21 0.1723484405344065 22-23 0.19055195488897728 24-25 0.2188165375886193 26-27 0.21081447857928354 28-29 0.2091766302440394 30-31 0.2228409649266478 32-33 0.2139029925828868 34-35 0.19995788389995084 36-37 0.18708907555160392 38-39 0.18180116521210138 40-41 0.18488967921570462 42-43 0.177261985540139 44-45 0.1921430075575002 46-47 0.21886333325534055 48-49 0.22260698659304148 50-51 0.23369755960598051 52-53 0.26247689463955637 54-55 0.24848499028989915 56-57 0.2232153302604179 58-59 0.17964856454292333 60-61 0.18905449355389692 62-63 0.1960270478953649 64-65 0.18264348721308407 66-67 0.19434240389339946 68-69 0.18025690821029974 70-71 0.19476356489389082 72-73 0.21549404525140972 74-75 0.24506890661924707 76-77 0.24754907695547393 78-79 0.24188680128220127 80-81 0.246847141954655 82-83 0.23599054727532232 84-85 0.20613491190715738 86-87 0.21802101125435783 88-89 0.22045438592386343 90-91 0.20814712557617165 92-93 0.20693043824141885 94-95 0.2435246496174454 96-97 0.2726783499847914 98-99 0.2911158426729685 100-101 0.2877933503357589 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1068475.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.13002612765186 #Duplication Level Percentage of deduplicated Percentage of total 1 85.27382094393664 63.2135057457873 2 9.139672098028067 13.550482628499827 3 2.741565951890119 6.096970669328859 4 1.1223829287488374 3.32809103333527 5 0.5536689887026854 2.052174829930033 6 0.3317600916527353 1.4756030557397666 7 0.2034370233514945 1.0556554299464604 8 0.13788249961271482 0.8176986639069197 9 0.08792639706160532 0.5866187500338385 >10 0.38106395768353724 4.639147420806379 >50 0.017243747259360717 0.8547419892762208 >100 0.00824955159537352 1.1278534071196733 >500 7.365669315582479E-4 0.36179103568868065 >1k 5.892535452465983E-4 0.8396653406007442 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2889 0.2703853623154496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1983 0.18559161421652356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1430 0.1338356068228082 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1387 0.12981117948477972 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 9.359133344252322E-5 0.0 0.0 0.0 0.0 6 9.359133344252322E-5 0.0 0.0 0.0 0.0 7 1.8718266688504644E-4 0.0 0.0 0.0 0.0 8 1.8718266688504644E-4 0.0 0.0 0.0 9.359133344252322E-5 9 1.8718266688504644E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 10-11 1.8718266688504644E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 12-13 1.8718266688504644E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 14-15 2.8077400032756964E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 16-17 3.743653337700929E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 18-19 3.743653337700929E-4 0.0 0.0 9.359133344252322E-5 9.359133344252322E-5 20-21 3.743653337700929E-4 0.0 0.0 3.743653337700929E-4 9.359133344252322E-5 22-23 3.743653337700929E-4 0.0 0.0 0.001076300334589017 9.359133344252322E-5 24-25 4.211610004913545E-4 0.0 0.0 0.0023397833360630804 9.359133344252322E-5 26-27 4.679566672126161E-4 0.0 0.0 0.00435199700507733 9.359133344252322E-5 28-29 7.019350008189241E-4 0.0 0.0 0.010388638012120078 9.359133344252322E-5 30-31 8.42322000982709E-4 0.0 0.0 0.024567725028662345 9.359133344252322E-5 32-33 9.359133344252323E-4 9.359133344252322E-5 0.0 0.055874026065186363 9.359133344252322E-5 34-35 0.0010295046678677556 9.359133344252322E-5 0.0 0.09948758744940218 9.359133344252322E-5 36-37 0.0010295046678677556 9.359133344252322E-5 0.0 0.16528229485949603 9.359133344252322E-5 38-39 0.0010295046678677556 9.359133344252322E-5 0.0 0.2635531949741454 9.359133344252322E-5 40-41 0.0010295046678677556 9.359133344252322E-5 0.0 0.3729146681017338 9.359133344252322E-5 42-43 0.0010295046678677556 9.359133344252322E-5 0.0 0.4772222092234259 9.359133344252322E-5 44-45 0.0010295046678677556 9.359133344252322E-5 0.0 0.6140527387163949 9.359133344252322E-5 46-47 0.0010295046678677556 9.359133344252322E-5 0.0 0.7819555909122815 9.359133344252322E-5 48-49 0.0010295046678677556 9.359133344252322E-5 0.0 0.9407332880975222 9.359133344252322E-5 50-51 0.0010295046678677556 9.359133344252322E-5 0.0 1.1134560939656988 9.359133344252322E-5 52-53 0.0010295046678677556 9.359133344252322E-5 0.0 1.284821825498959 1.4038700016378482E-4 54-55 0.0010295046678677556 9.359133344252322E-5 0.0 1.4735487493858068 1.8718266688504644E-4 56-57 0.0010295046678677556 9.359133344252322E-5 0.0 1.6980275626476988 1.8718266688504644E-4 58-59 0.0010295046678677556 9.359133344252322E-5 0.0 1.930602026252369 1.8718266688504644E-4 60-61 0.0010295046678677556 9.359133344252322E-5 0.0 2.1898968155548797 1.8718266688504644E-4 62-63 0.0010295046678677556 9.359133344252322E-5 0.0 2.449519174524439 1.8718266688504644E-4 64-65 0.0010295046678677556 9.359133344252322E-5 0.0 2.7216827721752965 1.8718266688504644E-4 66-67 0.0010295046678677556 9.359133344252322E-5 0.0 3.0251994665293997 1.8718266688504644E-4 68-69 0.0010295046678677556 9.359133344252322E-5 0.0 3.3697559605980487 1.8718266688504644E-4 70-71 0.0010295046678677556 9.359133344252322E-5 0.0 3.742249467699291 1.8718266688504644E-4 72-73 0.0010763003345890172 9.359133344252322E-5 0.0 4.122557851142984 1.8718266688504644E-4 74-75 0.0011230960013102786 9.359133344252322E-5 0.0 4.520274222606986 1.8718266688504644E-4 76-77 0.0011230960013102786 9.359133344252322E-5 0.0 4.935117808090971 1.8718266688504644E-4 78-79 0.0011230960013102786 9.359133344252322E-5 0.0 5.426097943330448 1.8718266688504644E-4 80-81 0.0012166873347528018 9.359133344252322E-5 0.0 5.9535786986125085 1.8718266688504644E-4 82-83 0.0012166873347528018 9.359133344252322E-5 0.0 6.476754252556214 1.8718266688504644E-4 84-85 0.0013570743349165866 9.359133344252322E-5 0.0 7.011441540513349 1.8718266688504644E-4 86-87 0.0014974613350803715 9.359133344252322E-5 0.0 7.566672126161118 1.8718266688504644E-4 88-89 0.0014974613350803715 9.359133344252322E-5 0.0 8.168183626196214 1.8718266688504644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4045 0.0 32.189747 1 GTATCAA 8020 0.0 30.041796 1 GTATTAG 220 5.6341756E-5 27.961529 1 TACCGTA 70 0.008663484 26.965792 7 GGTATCA 6025 0.0 25.184715 1 GTATAGT 330 1.2493674E-6 24.854692 1 TACATGG 4860 0.0 24.585648 2 ACATGGG 4850 0.0 23.857325 3 TATCAAC 10265 0.0 23.694399 2 GTATAGG 315 2.450075E-5 22.783468 1 GAGTACT 4875 0.0 22.749132 12-13 GTTATAC 230 0.002390601 22.288176 1 CATGGGA 3060 0.0 22.069456 4 GGTCTAG 280 2.896216E-4 21.969774 1 CTACTAG 235 0.002708572 21.813961 1 CAACGCA 11190 0.0 21.760597 5 TCAACGC 11200 0.0 21.757486 4 ATCAACG 11120 0.0 21.660217 3 AACGCAG 11460 0.0 21.33027 6 ATGGGAG 1360 0.0 21.166164 5 >>END_MODULE