FastQCFastQC Report
Fri 27 May 2016
SRR939664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068475
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69500.6504597674255365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56540.5291653992840263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38600.36126254708813965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36080.3376775310606238No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21210.19850721823159176No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18170.17005545286506468No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16660.15592316151524369No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16160.15124359484311753No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13560.1269098481480615No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.10978263412807973No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA650.00606684229.0076276
CGTTAGG700.00866523326.9646761
GTATCAA95650.024.5191081
GGTATCA69450.024.3245071
GTACATG50500.023.2670441
TACATGG52350.022.4385032
GAGTACT52650.021.80048612-13
ACATGGG52100.021.2765253
AGTACTT55200.021.13497212-13
CATGGGA30950.020.4227714
ATCAACG113700.020.4115123
TCAACGC113250.020.4093154
CAACGCA113500.020.364365
GTACTTT56050.020.2678214-15
AACGCAG114850.020.1929456
TATCAAC115600.020.03712
AGAGTAC82050.020.02118510-11
AGTACAT39850.019.0593812
GTGTACG1250.006264662418.8752751
TACTTTT61000.018.6617714-15
Produced by FastQC (version 0.11.5)