##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939664_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1068475 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.460330845363718 31.0 31.0 34.0 30.0 34.0 2 31.65449823346358 31.0 31.0 34.0 30.0 34.0 3 31.6946564028171 31.0 31.0 34.0 30.0 34.0 4 35.51310419055196 37.0 35.0 37.0 33.0 37.0 5 35.265016027515856 37.0 35.0 37.0 33.0 37.0 6 35.27618802498888 37.0 35.0 37.0 32.0 37.0 7 35.18459205877536 37.0 35.0 37.0 32.0 37.0 8 35.149597323287864 37.0 35.0 37.0 32.0 37.0 9 36.73431947401671 39.0 35.0 39.0 32.0 39.0 10-11 36.61622452560893 39.0 35.0 39.0 32.0 39.0 12-13 36.55217482861087 39.0 35.0 39.0 32.0 39.0 14-15 37.5746007159737 39.0 36.0 41.0 32.0 41.0 16-17 37.53909871545895 39.0 36.0 41.0 32.0 41.0 18-19 37.54505206017923 39.0 36.0 41.0 32.0 41.0 20-21 37.49289173822504 39.0 36.0 41.0 32.0 41.0 22-23 37.341881185802194 39.0 36.0 41.0 31.5 41.0 24-25 37.19849224361824 39.0 36.0 40.5 31.0 41.0 26-27 36.89115655490302 39.0 36.0 40.0 31.0 41.0 28-29 36.68908537869393 38.5 35.0 40.0 30.0 41.0 30-31 36.399374809892606 38.0 35.0 40.0 30.0 41.0 32-33 36.15452069538361 38.0 35.0 40.0 30.0 41.0 34-35 36.06102856875454 38.0 35.0 40.0 30.0 41.0 36-37 35.92938112730761 38.0 35.0 40.0 29.5 41.0 38-39 35.93869580476848 38.0 35.0 40.0 29.5 41.0 40-41 35.806147078780505 38.0 35.0 40.0 29.0 41.0 42-43 35.55890217365872 38.0 34.5 40.0 27.5 41.0 44-45 35.271745244390374 38.0 34.0 40.0 27.0 41.0 46-47 34.87732235195021 38.0 34.0 40.0 25.5 41.0 48-49 34.49252953976462 38.0 33.0 40.0 24.0 41.0 50-51 34.196918037389736 38.0 33.0 40.0 24.0 41.0 52-53 33.94813729848616 37.5 33.0 40.0 23.0 41.0 54-55 33.65455626009032 37.0 33.0 40.0 22.5 41.0 56-57 32.92322188165376 36.5 31.5 40.0 18.0 41.0 58-59 29.946812513161284 34.5 25.5 39.0 7.5 40.0 60-61 29.65450384894359 34.0 25.5 38.0 8.0 40.0 62-63 31.28174922202204 35.0 29.5 38.0 8.5 40.0 64-65 31.27972952104635 35.0 30.5 38.5 4.5 40.0 66-67 26.625395072416296 31.5 18.5 36.5 2.0 39.5 68-69 19.230844427805984 19.0 7.5 31.5 2.0 36.5 70-71 17.310777510002573 15.0 7.0 28.5 2.0 34.5 72-73 19.033537518425796 19.0 7.5 30.5 2.0 35.0 74-75 20.47563677203491 22.5 7.5 32.0 2.0 35.5 76-77 22.28090315636772 27.0 11.0 33.0 2.0 35.5 78-79 24.489389082570952 30.5 12.5 34.5 2.0 36.0 80-81 26.018619059875057 31.5 22.0 35.0 2.0 36.0 82-83 27.017896066824214 33.0 26.0 35.0 2.0 36.0 84-85 27.029653946044597 33.0 26.0 35.0 2.0 36.0 86-87 26.77296380355179 33.0 25.5 35.0 2.0 35.0 88-89 26.34951215517443 33.0 25.0 35.0 2.0 35.0 90-91 24.676415451929152 32.0 12.5 34.5 2.0 35.0 92-93 23.229859379021505 31.0 2.0 34.0 2.0 35.0 94-95 19.53353003111912 27.0 2.0 34.0 2.0 35.0 96-97 16.868948735347104 16.5 2.0 33.0 2.0 35.0 98-99 17.151380238189944 19.5 2.0 33.0 2.0 35.0 100-101 16.39266384332811 9.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25197423205557 1101 2 0.1632005554775624 1101 3 0.1371724463522206 1101 4 0.15743740729697464 1101 5 0.18803600194566172 1101 6 0.33574417521961664 1101 7 0.4085414996613892 1101 8 0.423596467341973 1101 9 0.21201462108817992 1101 10-11 0.34381037533131575 1101 12-13 0.17397321006775002 1101 14-15 0.3828784518308268 1101 16-17 0.5282495810294421 1101 18-19 0.3612575985389128 1101 20-21 0.45745216139270184 1101 22-23 0.510110410662385 1101 24-25 0.46086702370114097 1101 26-27 0.5570783621886832 1101 28-29 0.3950909166364198 1101 30-31 0.31036167238395507 1101 32-33 0.1781393488228673 1101 34-35 0.20204984256213976 1101 36-37 0.055907540351121554 1101 38-39 0.14588292172521733 1101 40-41 0.10371057646197812 1101 42-43 0.20788110885357725 1101 44-45 0.008459721185374747 1101 46-47 0.12465739713007196 1101 48-49 0.08305123556047533 1101 50-51 0.15320633758686597 1101 52-53 -0.29322679157406384 1101 54-55 -0.29552790862999956 1101 56-57 -3.5693529529607666 1101 58-59 -10.027360605209093 1101 60-61 -11.821568805743507 1101 62-63 -4.229407347545909 1101 64-65 -1.341580000874199 1101 66-67 -2.1130906406098653 1101 68-69 -4.271992117341643 1101 70-71 -4.434439281517566 1101 72-73 -3.4640492011698285 1101 74-75 -6.060031961700153 1101 76-77 -4.8055155267716145 1101 78-79 0.9239537424721505 1101 80-81 1.5653912410413113 1101 82-83 0.1742799729825535 1101 84-85 -0.06504081860791189 1101 86-87 -0.05678355607918206 1101 88-89 -0.5295941103644957 1101 90-91 0.8129984424486913 1101 92-93 0.6354103053733269 1101 94-95 -7.950890427500678 1101 96-97 -9.108536076667358 1101 98-99 -8.483573680216338 1101 100-101 -9.371495107751276 1102 1 0.17365262537595072 1102 2 0.46097633307728003 1102 3 0.3632725747782892 1102 4 0.24571916896599788 1102 5 0.3152919649765167 1102 6 0.39032927852759514 1102 7 0.20740725234424673 1102 8 0.34029661259880584 1102 9 0.22327294042636225 1102 10-11 0.3499096988076644 1102 12-13 0.34404554577567836 1102 14-15 0.5244369076445778 1102 16-17 0.4094532735532894 1102 18-19 0.3983752931629496 1102 20-21 0.38786499082751646 1102 22-23 0.3981064151847349 1102 24-25 0.4109916079629059 1102 26-27 0.1867308800347871 1102 28-29 0.1281051971760263 1102 30-31 0.36351067223393585 1102 32-33 0.02037945068558855 1102 34-35 0.11772733580239247 1102 36-37 0.11157749633500913 1102 38-39 0.17622696454853326 1102 40-41 0.11772255557718836 1102 42-43 0.16204471122305364 1102 44-45 0.08963048394839745 1102 46-47 0.2994171013476503 1102 48-49 0.11950923730941554 1102 50-51 0.09364664507765497 1102 52-53 0.02279353706494902 1102 54-55 -0.0859610813392706 1102 56-57 -3.3646575349540413 1102 58-59 -9.772544627689143 1102 60-61 -7.709139284578821 1102 62-63 -2.32335229630354 1102 64-65 -0.6464989753682708 1102 66-67 -1.7982254148031735 1102 68-69 -4.840647741561847 1102 70-71 -4.476981971293608 1102 72-73 -3.0583340582363725 1102 74-75 -4.091756859160537 1102 76-77 -4.171130636409423 1102 78-79 1.4848101357852137 1102 80-81 1.8707961124505985 1102 82-83 0.46058704666106465 1102 84-85 0.05978309864325837 1102 86-87 0.009851278485360382 1102 88-89 -0.011884484995405842 1102 90-91 1.1665378429075126 1102 92-93 0.6563071657035771 1102 94-95 -9.056260905604217 1102 96-97 -9.360771824483933 1102 98-99 -8.641017086763506 1102 100-101 -9.353291901856117 1103 1 0.3042639388517152 1103 2 0.1949443164058735 1103 3 0.2714270091475548 1103 4 0.1408921261594145 1103 5 0.1655442749474929 1103 6 0.32034555381241603 1103 7 0.212266156819517 1103 8 0.1823983632683337 1103 9 0.11571182199567431 1103 10-11 0.22255128947995217 1103 12-13 0.2229927329070165 1103 14-15 0.33080622744100197 1103 16-17 0.4315002565977153 1103 18-19 0.3827641519124185 1103 20-21 0.39943529747454676 1103 22-23 0.3642501629781023 1103 24-25 0.4938437870427137 1103 26-27 0.5265538755483234 1103 28-29 0.36362966607644864 1103 30-31 0.4166755262299944 1103 32-33 0.43337879622969666 1103 34-35 0.2509306390824264 1103 36-37 0.15012258059621075 1103 38-39 0.3027802208971906 1103 40-41 0.4894459704591725 1103 42-43 0.4162017978609569 1103 44-45 0.3236329123242285 1103 46-47 0.23827714250948162 1103 48-49 0.17512830092080378 1103 50-51 0.14501389817124988 1103 52-53 0.4502404271077509 1103 54-55 0.519539792703803 1103 56-57 1.0508046314672015 1103 58-59 -7.794387093265623 1103 60-61 -7.087852891233602 1103 62-63 -1.9308531359015682 1103 64-65 -0.12418827243097041 1103 66-67 -1.629689027916406 1103 68-69 -3.288959718965897 1103 70-71 -3.8328251429935563 1103 72-73 -2.796352563837999 1103 74-75 -3.6264677791016346 1103 76-77 -0.3563814824658955 1103 78-79 3.347443287420923 1103 80-81 2.8434865720336404 1103 82-83 1.3461842807237687 1103 84-85 0.8073418059206361 1103 86-87 0.7646854255829325 1103 88-89 0.589386106684394 1103 90-91 2.0393914859018203 1103 92-93 1.5935622829459035 1103 94-95 -7.354330045425275 1103 96-97 -9.109648054817963 1103 98-99 -8.47380575778747 1103 100-101 -9.652997181420732 1104 1 0.26827681252320446 1104 2 0.1855231550280081 1104 3 0.20583983011324847 1104 4 0.21446927987811648 1104 5 0.2515002824272301 1104 6 0.11644981036629787 1104 7 0.21687861501232675 1104 8 0.1861291207459672 1104 9 0.23610041511533097 1104 10-11 0.11333183991911255 1104 12-13 0.04281319677411233 1104 14-15 0.15782958339858766 1104 16-17 0.167453320402565 1104 18-19 0.22047970601015265 1104 20-21 0.2790043014190715 1104 22-23 0.2332885204635815 1104 24-25 0.17046953662849518 1104 26-27 0.13981842501187458 1104 28-29 0.3353206250696559 1104 30-31 0.28604609845235274 1104 32-33 0.49975831442020535 1104 34-35 0.23798127309392214 1104 36-37 -0.003956021130193221 1104 38-39 -0.18932021728983983 1104 40-41 0.034986373097829926 1104 42-43 0.023287083131087627 1104 44-45 0.1518484566097129 1104 46-47 0.14461501470913163 1104 48-49 -0.05375678922573712 1104 50-51 0.27778099461445294 1104 52-53 0.18854948213203926 1104 54-55 0.002097818142758001 1104 56-57 0.44791162984397204 1104 58-59 -7.9838167772831525 1104 60-61 -7.347414578043416 1104 62-63 -2.200172053638468 1104 64-65 -0.36927759193744336 1104 66-67 -1.6207791548308457 1104 68-69 -3.363498122709128 1104 70-71 -3.4963140425357455 1104 72-73 -2.8415950946134174 1104 74-75 -3.6638703224367326 1104 76-77 -0.03586469931380876 1104 78-79 3.5740207467211143 1104 80-81 2.892060361552474 1104 82-83 1.3483198467644186 1104 84-85 0.7435628843149047 1104 86-87 0.8053996031622219 1104 88-89 0.8867091848151283 1104 90-91 2.288358746630113 1104 92-93 2.0284611607049 1104 94-95 -5.933361758616023 1104 96-97 -7.674045148149277 1104 98-99 -5.716142390840176 1104 100-101 -5.097693590417723 1105 1 0.1606861023208559 1105 2 0.15594978496167755 1105 3 0.13390243454244555 1105 4 0.22003930262202687 1105 5 0.21942480282130816 1105 6 0.31579501486589834 1105 7 0.25867543980598384 1105 8 0.2567351313970434 1105 9 0.22182609673411235 1105 10-11 0.2020498545489815 1105 12-13 0.18549386277391733 1105 14-15 0.2974440825016771 1105 16-17 0.38616717183510474 1105 18-19 0.44541809627776985 1105 20-21 0.3645520692188384 1105 22-23 0.517893701269287 1105 24-25 0.4649982850428458 1105 26-27 0.483905637010821 1105 28-29 0.5222191967297434 1105 30-31 0.40660558746878195 1105 32-33 0.4488593851163287 1105 34-35 0.4523562486456214 1105 36-37 0.24745778950859432 1105 38-39 0.25657148928577556 1105 40-41 0.17919016859263337 1105 42-43 0.19727031298987896 1105 44-45 0.34258925426713915 1105 46-47 0.0784970146085513 1105 48-49 0.005467486964690238 1105 50-51 -0.16404565711314234 1105 52-53 0.16867335820847273 1105 54-55 0.3340537804560384 1105 56-57 0.8681898934833399 1105 58-59 -8.637283337588045 1105 60-61 -7.563100332085067 1105 62-63 -2.1936827745701315 1105 64-65 -0.37456960251028804 1105 66-67 -1.530288987241196 1105 68-69 -3.2794787233271823 1105 70-71 -3.881992713553746 1105 72-73 -2.873297912175964 1105 74-75 -3.4285574679362902 1105 76-77 -0.2359786284220995 1105 78-79 3.5752103314631363 1105 80-81 2.9565382295483857 1105 82-83 1.4640378725706498 1105 84-85 1.1667962496871596 1105 86-87 1.153565840470062 1105 88-89 0.8414085684383998 1105 90-91 2.184270081703236 1105 92-93 1.8051171492826477 1105 94-95 -6.38279986668033 1105 96-97 -7.696209982822843 1105 98-99 -5.577941353114342 1105 100-101 -4.916376217870237 1106 1 0.09266370806989599 1106 2 0.08501725379338865 1106 3 0.289103776824323 1106 4 0.10420169241015742 1106 5 0.11068838441385509 1106 6 0.2513693467287865 1106 7 0.3745001764704199 1106 8 0.20900931507109988 1106 9 0.24032323880658168 1106 10-11 0.08776136121176137 1106 12-13 0.23705492343979273 1106 14-15 0.17746333560706518 1106 16-17 0.15028873817391997 1106 18-19 0.356602062131401 1106 20-21 0.19562287659051236 1106 22-23 0.23698109549164315 1106 24-25 0.35216807855115206 1106 26-27 0.19951860381995346 1106 28-29 0.3544479081734693 1106 30-31 0.4385319851069127 1106 32-33 0.3503027243428818 1106 34-35 0.26947499138636744 1106 36-37 0.2671614690545283 1106 38-39 0.3301059074300383 1106 40-41 0.28652050954138275 1106 42-43 0.5686943275913379 1106 44-45 0.4604744737182145 1106 46-47 0.5445151405633339 1106 48-49 0.4634990071303804 1106 50-51 0.26329711396086 1106 52-53 0.5134158729558322 1106 54-55 0.4055137694706161 1106 56-57 0.7806962587373505 1106 58-59 -8.02915161831148 1106 60-61 -6.889872447409168 1106 62-63 -1.5641877609241703 1106 64-65 0.3777976998235424 1106 66-67 0.9647754377117437 1106 68-69 -2.446359591519222 1106 70-71 -2.5225915194607005 1106 72-73 -1.0941396848348361 1106 74-75 -1.2793101413429717 1106 76-77 2.0556383320475256 1106 78-79 4.359490442690586 1106 80-81 3.3686648251530684 1106 82-83 1.6933236195510908 1106 84-85 1.2149408139032758 1106 86-87 0.9697830772918401 1106 88-89 1.0797608687982851 1106 90-91 2.35238609525193 1106 92-93 1.7053267735467124 1106 94-95 -6.699057154538572 1106 96-97 -7.764437638635679 1106 98-99 -5.596686310974929 1106 100-101 -4.961561296570524 1107 1 0.08147290797557716 1107 2 0.06925479709933313 1107 3 -0.058130265728870256 1107 4 -0.07218545819475963 1107 5 -0.05933501439987765 1107 6 -0.06869377565244861 1107 7 0.06457291231776452 1107 8 0.15390420179544861 1107 9 -0.0643619655249168 1107 10-11 -0.021557171011494347 1107 12-13 0.1769464545203121 1107 14-15 -0.00983636873350946 1107 16-17 0.07245376329590414 1107 18-19 -0.029232767637694224 1107 20-21 0.22465880918742442 1107 22-23 0.21683308439080662 1107 24-25 0.20735227618949637 1107 26-27 0.038868153075362954 1107 28-29 0.13767150288424546 1107 30-31 0.08721675411164398 1107 32-33 0.05957300784944408 1107 34-35 -0.10519515789443545 1107 36-37 -0.06685750393707224 1107 38-39 0.15257820069517436 1107 40-41 -0.047314900663401716 1107 42-43 -0.28261473627472355 1107 44-45 -0.20461123123907043 1107 46-47 -0.12045629111756995 1107 48-49 -0.1551772367251658 1107 50-51 0.02547918938122251 1107 52-53 -0.050788658070267445 1107 54-55 -0.01880249542572443 1107 56-57 0.7121446271689038 1107 58-59 -8.064170029006007 1107 60-61 -7.211058350077359 1107 62-63 -2.03812801232538 1107 64-65 -0.18856931691839662 1107 66-67 0.4352464975510344 1107 68-69 -2.598303869693023 1107 70-71 -2.4699739358870296 1107 72-73 -1.108832325998609 1107 74-75 -1.039726966722526 1107 76-77 1.8709030651377994 1107 78-79 4.000107518383025 1107 80-81 2.981497532704175 1107 82-83 1.346129119179249 1107 84-85 1.0389149845150918 1107 86-87 0.8873256576912496 1107 88-89 0.6454839646971458 1107 90-91 1.8636385666382616 1107 92-93 1.6235081959769246 1107 94-95 -6.105889970921371 1107 96-97 -7.769783451336339 1107 98-99 -5.698021675833177 1107 100-101 -4.866545788054443 1108 1 0.05851902796919006 1108 2 0.013481368887880762 1108 3 -0.07036244502722155 1108 4 -0.044517011084579394 1108 5 0.046378612203852754 1108 6 0.017037241329788344 1108 7 -0.1402112163000524 1108 8 -0.060991189754247443 1108 9 -0.1684061157919814 1108 10-11 -0.14062944755400508 1108 12-13 -0.13463553756529478 1108 14-15 -0.1874265591404054 1108 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2312 98-99 -0.2650681899065752 2312 100-101 0.26285061560499123 2313 1 -0.2084483880750767 2313 2 -0.27725481539425445 2313 3 -0.1489677111224772 2313 4 -0.089334076011923 2313 5 -0.10100106204671988 2313 6 -0.11447648200712734 2313 7 -0.020735094261532083 2313 8 -0.10040682913786014 2313 9 -0.2260747049529428 2313 10-11 -0.25200516450293975 2313 12-13 -0.14608037556758546 2313 14-15 -0.3688020750351271 2313 16-17 -0.2498288280979537 2313 18-19 -0.12705452530027372 2313 20-21 -0.09343920818050577 2313 22-23 -0.1051707712183898 2313 24-25 -0.02553642404573253 2313 26-27 -0.2235605269975025 2313 28-29 -0.19225545277625145 2313 30-31 -0.3041371306799192 2313 32-33 -0.41080388679338853 2313 34-35 -0.4462867551424523 2313 36-37 -0.4494506664209368 2313 38-39 -0.30740517420092317 2313 40-41 -0.37794258676728987 2313 42-43 -0.4357298209035818 2313 44-45 -0.5645758586775642 2313 46-47 -0.39490421386390295 2313 48-49 -0.44187821477648725 2313 50-51 -0.4802946738330789 2313 52-53 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98-99 6.482694684634474 2315 100-101 6.576773506114462 2316 1 -0.3763412852317707 2316 2 -0.3284872784542934 2316 3 -0.4801952471330857 2316 4 -0.34401237582583377 2316 5 -0.44664759345555183 2316 6 -0.45962809572645114 2316 7 -0.22462643069418675 2316 8 -0.4598321345522294 2316 9 -0.5563780504047529 2316 10-11 -0.598278272032843 2316 12-13 -0.5509385348130778 2316 14-15 -0.4851147391010784 2316 16-17 -0.6345469352258775 2316 18-19 -0.6343595399007214 2316 20-21 -0.5303718691338659 2316 22-23 -0.8069408211010867 2316 24-25 -0.559857601286005 2316 26-27 -0.6296043497092825 2316 28-29 -0.6350087440526408 2316 30-31 -0.5811715484804338 2316 32-33 -0.48690172265330744 2316 34-35 -0.49852786192887066 2316 36-37 -0.46499226200311483 2316 38-39 -0.453224816163754 2316 40-41 -0.5436463398070543 2316 42-43 -0.6933931160817863 2316 44-45 -0.5071908532821467 2316 46-47 -0.5487830482774214 2316 48-49 -0.4675342757479868 2316 50-51 -0.2646270992026629 2316 52-53 -0.5614488555442634 2316 54-55 -0.3255003753177377 2316 56-57 0.41078307607297404 2316 58-59 3.530628815601215 2316 60-61 3.3568436303262708 2316 62-63 0.8149030058173068 2316 64-65 0.45416737812965025 2316 66-67 -1.3808149723653322 2316 68-69 -2.8156638349557266 2316 70-71 -2.523026476052033 2316 72-73 -0.038178169794036876 2316 74-75 6.283036536458582 2316 76-77 5.994831400579283 2316 78-79 5.011064115638025 2316 80-81 3.3484306445242957 2316 82-83 1.7984091320169497 2316 84-85 1.0845930226128466 2316 86-87 0.9213463112810842 2316 88-89 1.0365783784108267 2316 90-91 2.6038518989647166 2316 92-93 4.366504288030768 2316 94-95 6.868074045767639 2316 96-97 4.288774950747898 2316 98-99 6.600818778017189 2316 100-101 6.628239841275919 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 65.0 6 169.0 7 381.0 8 858.0 9 1317.0 10 2176.0 11 3393.0 12 4954.0 13 6405.0 14 8074.0 15 10243.0 16 13569.0 17 17230.0 18 17864.0 19 16257.0 20 16543.0 21 16924.0 22 16824.0 23 17045.0 24 18225.0 25 18953.0 26 24215.0 27 33395.0 28 46172.0 29 60140.0 30 72950.0 31 82809.0 32 86819.0 33 87129.0 34 89192.0 35 94574.0 36 91999.0 37 66242.0 38 24012.0 39 1328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.00127130465065 19.686873358210214 14.582650717004588 22.729204620134542 2 15.949861887115299 22.04920019956399 38.2922302024938 23.70870771082691 3 18.981124446873665 26.336955952051156 30.80380206500498 23.8781175360702 4 12.97550246847142 17.580944804511102 36.983083366480265 32.46046936053722 5 13.089969189275246 38.1757990653768 34.09033442279057 14.643897322557384 6 30.531832752287137 36.87142890568333 17.488570158403334 15.108168183626198 7 26.68354430379747 33.88277685486324 21.817262921453473 17.61641591988582 8 25.82718360279838 33.81847960878822 20.607407754041976 19.746929034371416 9 26.3576592807506 16.20056622756733 20.97147804113339 36.47029645054868 10-11 24.638012120077683 26.465616883876553 27.63401109057301 21.262359905472753 12-13 25.996771098996234 24.387374529118603 28.073235218418773 21.54261915346639 14-15 23.04009815946327 25.523051516403665 26.91504779517916 24.521802528953906 16-17 21.390018484288355 28.610402676712138 27.664475069608553 22.335103769390955 18-19 21.346608396402516 27.02444945784601 30.12552066296698 21.50342148278449 20-21 22.52640445494747 25.985586934649852 29.891293666206508 21.59671494419617 22-23 22.076932076089754 26.01230726034769 30.081518051428436 21.829242612134117 24-25 22.175925665949755 26.214255096520834 30.063015103783524 21.546804133745884 26-27 21.765209899917966 26.174069829527824 30.506839898228645 21.553880372325565 28-29 21.637165553306737 26.747967289446283 30.138680580741017 21.47618657650596 30-31 21.828677325683426 26.367799255444357 29.855370885581312 21.948152533290905 32-33 21.675612940664443 26.365270049559392 29.870696311826393 22.08842069794977 34-35 22.21168258609289 26.419118847942375 29.782531481248363 21.586667084716368 36-37 22.27106326614346 26.03393653713928 29.925238074718834 21.769762121998422 38-39 21.867894351074966 26.58762589658507 29.768614897538153 21.775864854801807 40-41 21.915202042082832 26.53452454831797 29.851082514665762 21.699190894933434 42-43 21.77320049255569 26.685455227596233 29.148889431176865 22.39245484867121 44-45 21.859790185060017 26.40818500287363 29.210292239226693 22.52173257283966 46-47 22.405072362285896 26.70090832830216 28.827351112705685 22.066668196706264 48-49 22.039535769197805 26.894560822693187 28.667015418940622 22.398887989168383 50-51 21.866863229195154 27.05884887288748 28.846643763510553 22.22764413440681 52-53 22.170085422460257 27.225055382017686 28.17321050879346 22.431648686728593 54-55 21.885606065926453 27.107424860653644 28.16193907386469 22.845029999555216 56-57 21.928596749442388 27.162203358637925 28.02960876143612 22.879591130483565 58-59 21.09327123421988 26.712832454957713 29.648524046121793 22.545372264700614 60-61 21.16409595125236 27.111647909507315 29.526489341619484 22.197766797620847 62-63 21.71320483470324 27.111392101770615 28.72607965617226 22.449323407353887 64-65 21.707530398020683 27.59302147644788 28.146674222787322 22.552773902744118 66-67 21.485341976383047 27.153675827901854 28.500532145718488 22.860450049996615 68-69 20.80838933495724 26.71189951594458 29.897075288719037 22.582635860379142 70-71 21.7542362725595 26.71864439278096 28.80895429379479 22.71816504086475 72-73 21.068294947834683 26.77215726915549 29.186167682197244 22.973380100812584 74-75 21.019348677806367 27.479797613408607 28.67427868601392 22.826575022771102 76-77 20.02076787374038 27.41986519744806 29.08685960417489 23.47250732463667 78-79 21.23543255200979 27.26221406382378 28.293419937870656 23.208933446295774 80-81 21.62165957966753 27.36210547308587 27.688967267473785 23.327267679772817 82-83 21.808495952403124 27.080456370700816 27.790160448527736 23.320887228368324 84-85 21.469050286440567 27.342041201393783 27.60620669581144 23.582701816354206 86-87 21.50503669303174 27.274186468549566 27.722776447201063 23.498000391217634 88-89 21.46888927573343 27.161353481694455 27.277406843779072 24.092350398793045 90-91 21.278634780620262 27.079145554627416 27.597948827750646 24.044270837001676 92-93 21.249410639898755 27.18412054362997 27.577885663906255 23.98858315256502 94-95 20.045705558291125 27.098736670393553 28.547969571212683 24.30758820010264 96-97 19.659917016477234 27.070601680518674 28.518743642602523 24.75073766040157 98-99 20.45934712900978 26.955082321363044 27.978193353334852 24.607377196292322 100-101 19.58342884874548 26.80670049834333 28.727819438449398 24.882051214461796 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1164.0 1 881.5 2 606.5 3 634.0 4 705.0 5 785.5 6 936.5 7 1284.5 8 1716.0 9 2057.5 10 2088.0 11 2019.5 12 2109.0 13 2164.0 14 2236.5 15 2384.0 16 2470.5 17 2614.0 18 2737.5 19 2887.0 20 3139.5 21 3367.5 22 3792.0 23 4262.5 24 4781.5 25 5597.0 26 6466.0 27 7497.5 28 8640.5 29 10045.0 30 11815.5 31 13745.5 32 16011.5 33 17970.0 34 19578.5 35 21651.0 36 24239.0 37 26637.0 38 28149.5 39 29164.5 40 29891.0 41 30577.0 42 31503.5 43 32900.5 44 34889.0 45 36993.5 46 39010.5 47 41495.0 48 43661.0 49 43268.5 50 41197.5 51 39637.0 52 38498.5 53 37081.5 54 35466.5 55 33533.5 56 31014.0 57 27910.5 58 24914.0 59 21903.5 60 18377.5 61 14799.0 62 11565.0 63 8595.5 64 6055.0 65 4317.0 66 3023.5 67 2050.0 68 1423.5 69 933.5 70 572.0 71 358.0 72 238.0 73 153.5 74 96.5 75 63.0 76 31.0 77 11.5 78 3.5 79 3.5 80 2.5 81 1.0 82 1.5 83 1.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026299164697349026 2 0.01366433468260839 3 2.807740003275697E-4 4 0.0 5 0.06223823673927794 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 8.891176677039706E-4 16-17 0.0 18-19 5.147523339338777E-4 20-21 0.0 22-23 0.0 24-25 9.359133344252322E-5 26-27 1.4038700016378485E-4 28-29 0.0011698916680315402 30-31 0.004913545005732469 32-33 0.005615480006551393 34-35 0.0020122136690142495 36-37 0.08919254077072464 38-39 0.08259435176302674 40-41 0.08774187510236552 42-43 0.09185989377383655 44-45 0.09457404244366972 46-47 0.09026884110531365 48-49 0.08044175109384871 50-51 0.07403074475303587 52-53 0.06219144107255668 54-55 0.050773298392568854 56-57 0.07024029574861368 58-59 0.7304803575188937 60-61 0.3487213084068415 62-63 0.0030885140036032662 64-65 0.002386579002784342 66-67 1.114064437633075 68-69 2.4074030744753037 70-71 2.37721986944009 72-73 1.8342029528065702 74-75 1.3063946278574605 76-77 0.7659046772268888 78-79 0.577926484007581 80-81 0.04113339104798896 82-83 1.4038700016378485E-4 84-85 0.0014974613350803715 86-87 0.0014974613350803715 88-89 9.359133344252322E-5 90-91 0.3388474227286553 92-93 0.05423617772994221 94-95 0.9731626851353564 96-97 1.6870305809682025 98-99 0.6603336531037226 100-101 0.585460586349704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1068475.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.516412979106995 #Duplication Level Percentage of deduplicated Percentage of total 1 72.09891775035744 34.9798086892301 2 13.936195353196142 13.52268418226352 3 5.418807314388453 7.887032805572276 4 2.677488030287381 5.196084600961533 5 1.5045550659248599 3.6497807464109013 6 0.9600732472044305 2.794758609093746 7 0.6632944322119934 2.2526466619957555 8 0.4759385702363718 1.8472665781018829 9 0.35554738255438106 1.5524895281084004 >10 1.7601491386112385 14.92816676348075 >50 0.0958862957379016 3.1703591547380623 >100 0.04715625615304173 4.056437167911878 >500 0.003672003212634166 1.1744932984694891 >1k 0.001932633269807456 1.8061842893515705 >5k 3.865266539614912E-4 1.1818069243101903 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6950 0.6504597674255365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5654 0.5291653992840263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3860 0.36126254708813965 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3608 0.3376775310606238 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2121 0.19850721823159176 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1817 0.17005545286506468 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1666 0.15592316151524369 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1616 0.15124359484311753 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1356 0.1269098481480615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.10978263412807973 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 9.359133344252322E-5 0.0 0.0 0.0 0.0 3 9.359133344252322E-5 0.0 0.0 0.0 0.0 4 9.359133344252322E-5 0.0 0.0 0.0 0.0 5 1.8718266688504644E-4 0.0 0.0 0.0 0.0 6 1.8718266688504644E-4 0.0 0.0 0.0 0.0 7 1.8718266688504644E-4 0.0 0.0 0.0 0.0 8 2.8077400032756964E-4 0.0 0.0 0.0 0.0 9 2.8077400032756964E-4 0.0 0.0 0.0 0.0 10-11 3.743653337700929E-4 0.0 0.0 0.0 0.0 12-13 3.743653337700929E-4 0.0 0.0 0.0 0.0 14-15 4.211610004913545E-4 0.0 0.0 0.0 0.0 16-17 4.679566672126161E-4 0.0 0.0 9.359133344252322E-5 0.0 18-19 4.679566672126161E-4 0.0 0.0 1.8718266688504644E-4 0.0 20-21 4.679566672126161E-4 0.0 0.0 4.6795666721261605E-4 0.0 22-23 4.679566672126161E-4 0.0 0.0 0.0012166873347528018 0.0 24-25 4.679566672126161E-4 0.0 0.0 0.0023397833360630804 0.0 26-27 4.679566672126161E-4 0.0 0.0 0.004211610004913545 0.0 28-29 6.083436673764009E-4 0.0 0.0 0.010388638012120078 0.0 30-31 6.551393340976625E-4 0.0 0.0 0.02555043402980884 0.0 32-33 6.551393340976625E-4 0.0 0.0 0.058447787734855754 0.0 34-35 6.551393340976625E-4 0.0 0.0 0.10463511078874096 0.0 36-37 8.42322000982709E-4 0.0 0.0 0.17356512786915934 0.0 38-39 8.42322000982709E-4 0.0 0.0 0.2791829476590468 0.0 40-41 8.42322000982709E-4 0.0 0.0 0.39317719179204 0.0 42-43 8.42322000982709E-4 0.0 0.0 0.5018367299188096 0.0 44-45 8.42322000982709E-4 0.0 0.0 0.6442359437516086 0.0 46-47 0.0010763003345890172 0.0 0.0 0.8185030066215868 0.0 48-49 0.0011230960013102786 0.0 0.0 0.9717588151337186 0.0 50-51 0.0011230960013102786 0.0 0.0 1.13353143498912 0.0 52-53 0.0011230960013102786 0.0 0.0 1.2888462528369873 4.679566672126161E-5 54-55 0.0011230960013102786 0.0 0.0 1.4618966283722128 9.359133344252322E-5 56-57 0.0011230960013102786 0.0 0.0 1.6326540162380963 9.359133344252322E-5 58-59 0.0011230960013102786 0.0 0.0 1.750625892042397 9.359133344252322E-5 60-61 0.0011230960013102786 0.0 0.0 1.8489903834904888 9.359133344252322E-5 62-63 0.0011230960013102786 0.0 0.0 1.9377149675940006 9.359133344252322E-5 64-65 0.0011230960013102786 9.359133344252322E-5 0.0 2.037155759376682 9.359133344252322E-5 66-67 0.0011698916680315402 9.359133344252322E-5 0.0 2.1199372938065935 9.359133344252322E-5 68-69 0.0012166873347528018 9.359133344252322E-5 0.0 2.2206415685907483 9.359133344252322E-5 70-71 0.0013570743349165866 9.359133344252322E-5 0.0 2.3786705351084487 9.359133344252322E-5 72-73 0.0014506656683591099 9.359133344252322E-5 0.0 2.584805447015606 9.359133344252322E-5 74-75 0.0014974613350803715 9.359133344252322E-5 0.0 2.8635672336741615 9.359133344252322E-5 76-77 0.0014974613350803715 9.359133344252322E-5 0.0 3.2156110344182127 9.359133344252322E-5 78-79 0.0014974613350803715 9.359133344252322E-5 0.0 3.668733475280189 9.359133344252322E-5 80-81 0.0015910526685228947 9.359133344252322E-5 0.0 4.199677109899623 9.359133344252322E-5 82-83 0.0015910526685228947 9.359133344252322E-5 0.0 4.713446734832354 9.359133344252322E-5 84-85 0.0015910526685228947 9.359133344252322E-5 0.0 5.174758417370551 9.359133344252322E-5 86-87 0.0015910526685228947 9.359133344252322E-5 0.0 5.593064882191909 9.359133344252322E-5 88-89 0.0015910526685228947 9.359133344252322E-5 0.0 6.00444558833852 9.359133344252322E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGA 65 0.006066842 29.007627 6 CGTTAGG 70 0.008665233 26.964676 1 GTATCAA 9565 0.0 24.519108 1 GGTATCA 6945 0.0 24.324507 1 GTACATG 5050 0.0 23.267044 1 TACATGG 5235 0.0 22.438503 2 GAGTACT 5265 0.0 21.800486 12-13 ACATGGG 5210 0.0 21.276525 3 AGTACTT 5520 0.0 21.134972 12-13 CATGGGA 3095 0.0 20.422771 4 ATCAACG 11370 0.0 20.411512 3 TCAACGC 11325 0.0 20.409315 4 CAACGCA 11350 0.0 20.36436 5 GTACTTT 5605 0.0 20.26782 14-15 AACGCAG 11485 0.0 20.192945 6 TATCAAC 11560 0.0 20.0371 2 AGAGTAC 8205 0.0 20.021185 10-11 AGTACAT 3985 0.0 19.059381 2 GTGTACG 125 0.0062646624 18.875275 1 TACTTTT 6100 0.0 18.66177 14-15 >>END_MODULE