FastQCFastQC Report
Fri 27 May 2016
SRR939663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335874
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135080.5782846163791369No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92370.3954408499773532No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT84510.36179177472757523No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67080.2871730238874186No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA45140.19324672478053184No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42780.18314344009993688No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA35440.15172051232215436No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34970.1497084174916969No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC30170.1291593639040462No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29900.12800347963974085No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28610.12248092148805971No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24600.10531389963670985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102850.025.6551861
TACATGG106000.024.5793912
GAGTACT91600.023.25996212-13
ACATGGG107550.023.0337563
AGTACTT96950.022.0253512-13
GTACTTT105300.020.16635714-15
ATGGGAG28600.020.0781655
CATGGGA72500.019.8994124
CATGGGG39300.018.8380834
TAGCCCT14900.018.4733814
AGTACAT76350.018.087922
TACTTTT118950.018.05165114-15
GTATCAA279700.017.6290041
ACTTTTT131400.016.99259216-17
GTGTAGC14150.016.434041
AGAGTAC203000.015.84863110-11
GTATCGT2406.205739E-515.8171266
TATCAAC313250.015.7560332
GTATATG10900.015.674031
CAACGCA312900.015.6523225
Produced by FastQC (version 0.11.5)