##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939663_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335874 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.298733578951605 31.0 30.0 33.0 26.0 34.0 2 30.440668460713205 31.0 30.0 34.0 26.0 34.0 3 30.48277432772487 31.0 30.0 34.0 26.0 34.0 4 34.075304575503644 35.0 35.0 37.0 30.0 37.0 5 34.096041567310564 35.0 35.0 37.0 30.0 37.0 6 34.216134089424344 36.0 35.0 37.0 30.0 37.0 7 34.03799562818885 36.0 35.0 37.0 29.0 37.0 8 34.04842170425288 36.0 35.0 37.0 29.0 37.0 9 35.31992008130575 38.0 35.0 39.0 29.0 39.0 10-11 35.22902305518191 37.0 35.0 39.0 28.5 39.0 12-13 35.09040384883774 37.0 34.5 39.0 27.0 39.0 14-15 35.75442532431116 38.0 34.0 40.0 27.0 41.0 16-17 35.51035415437647 38.0 34.0 40.0 27.0 41.0 18-19 35.54722771861839 38.0 34.0 40.0 27.0 41.0 20-21 35.66429439259138 38.0 34.0 40.0 27.0 41.0 22-23 35.51690587762867 38.0 34.0 40.0 27.0 41.0 24-25 35.38784733251879 38.0 34.0 40.0 27.0 41.0 26-27 35.504852359331025 38.0 34.0 40.0 27.0 41.0 28-29 35.37696189948602 38.0 34.0 40.0 26.5 41.0 30-31 35.139462359699195 38.0 34.0 40.0 25.0 41.0 32-33 34.880190241425694 38.0 34.0 40.0 25.0 41.0 34-35 34.635565745412634 38.0 33.0 40.0 24.5 41.0 36-37 34.31869805477521 38.0 33.0 40.0 24.0 41.0 38-39 34.14509965006674 38.0 33.0 40.0 23.5 41.0 40-41 33.87084513120142 38.0 33.0 40.0 22.0 41.0 42-43 33.52617628348105 38.0 32.5 40.0 20.0 41.0 44-45 33.239143678126474 37.0 32.0 40.0 18.5 41.0 46-47 32.728868081069436 37.0 31.0 40.0 14.5 41.0 48-49 32.33336900877359 37.0 31.0 40.0 11.0 41.0 50-51 31.67181384783597 36.0 30.0 39.0 9.0 40.5 52-53 31.69620257770753 36.0 30.0 39.0 8.5 40.0 54-55 31.737534858472674 36.0 30.0 39.0 8.0 40.0 56-57 31.619424891924822 36.0 30.0 39.0 7.0 40.0 58-59 31.24092866310426 36.0 29.5 39.0 3.5 40.0 60-61 30.814785600593183 35.0 29.0 39.0 2.0 40.0 62-63 30.41268814156928 35.0 28.0 38.5 2.0 40.0 64-65 29.808879246055223 34.5 28.0 38.0 2.0 40.0 66-67 29.269909678347375 34.0 26.5 38.0 2.0 40.0 68-69 28.76662739514203 34.0 26.0 37.0 2.0 39.0 70-71 28.209805837129913 33.5 26.0 36.5 2.0 39.0 72-73 27.466469295860993 33.0 25.0 36.0 2.0 38.5 74-75 27.022539957206597 32.0 24.0 35.0 2.0 38.0 76-77 26.658723458542713 32.0 23.5 35.0 2.0 37.5 78-79 26.102513234874827 32.0 21.5 35.0 2.0 37.0 80-81 25.43658283794417 31.0 20.0 34.5 2.0 36.0 82-83 24.782869495529297 31.0 18.0 34.0 2.0 36.0 84-85 24.125347086358254 31.0 13.0 34.0 2.0 35.0 86-87 23.47553335496692 30.0 7.0 34.0 2.0 35.0 88-89 22.688839166838623 29.5 2.0 34.0 2.0 35.0 90-91 21.469149877090974 28.5 2.0 33.5 2.0 35.0 92-93 19.87222919558161 26.0 2.0 33.0 2.0 34.0 94-95 19.616184991142504 26.0 2.0 33.0 2.0 34.0 96-97 18.98110857006842 25.5 2.0 32.0 2.0 34.0 98-99 18.004413979521154 24.5 2.0 32.0 2.0 34.0 100-101 16.358230152825023 18.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9065694572110203 1101 2 0.8984876506816022 1101 3 0.9338133947114642 1101 4 1.1078773411144098 1101 5 0.8376667361218608 1101 6 0.8901855319382221 1101 7 0.8702313697521262 1101 8 0.8153950844824607 1101 9 1.0736890383530948 1101 10-11 0.8395623339938396 1101 12-13 0.7483740282632922 1101 14-15 0.83320817535526 1101 16-17 0.798568901609876 1101 18-19 0.8363662197113584 1101 20-21 0.9841267720423801 1101 22-23 1.1428043393730931 1101 24-25 1.086625230986833 1101 26-27 1.0605588502601506 1101 28-29 1.0615103877338328 1101 30-31 1.1201937009581187 1101 32-33 1.0150294309948933 1101 34-35 0.9821214732770471 1101 36-37 0.9571954573425145 1101 38-39 1.102131847792002 1101 40-41 1.0707336207677116 1101 42-43 0.9329502797749711 1101 44-45 0.7416935791287287 1101 46-47 0.6850032313983192 1101 48-49 0.777450000966077 1101 50-51 0.7429055237850797 1101 52-53 0.9989955465590477 1101 54-55 1.0659661395880207 1101 56-57 0.9136704916675846 1101 58-59 0.721937053883849 1101 60-61 0.7990690385548511 1101 62-63 0.733815773781263 1101 64-65 0.5688351298964669 1101 66-67 0.41258302868937236 1101 68-69 0.3710483051546518 1101 70-71 0.4096022907002599 1101 72-73 0.4202941503202169 1101 74-75 0.16256606364594006 1101 76-77 -0.20440805109680227 1101 78-79 -0.024658127049757894 1101 80-81 0.1637437659391381 1101 82-83 -0.08415794499312312 1101 84-85 -0.12676334496580566 1101 86-87 -0.3595013738528934 1101 88-89 -1.0638617099973509 1101 90-91 -4.786988730256748 1101 92-93 -10.015691289431112 1101 94-95 -7.7924470214740005 1101 96-97 -4.624100028502012 1101 98-99 -3.4691323811081674 1101 100-101 -3.109470780829117 1102 1 1.036556877177599 1102 2 0.9141494793553129 1102 3 0.9718839555556436 1102 4 0.9606947053157668 1102 5 0.7864528567775224 1102 6 0.863540895976854 1102 7 0.9673742626591206 1102 8 0.8209077677848171 1102 9 1.0162977993136835 1102 10-11 0.907756129095759 1102 12-13 0.9251122321349428 1102 14-15 1.0899334862573298 1102 16-17 1.2288041670110275 1102 18-19 1.0540846647941962 1102 20-21 1.0056148460455248 1102 22-23 0.9833245644367778 1102 24-25 0.9199585643201686 1102 26-27 0.9392022094420156 1102 28-29 1.0503028922870499 1102 30-31 1.1008874178260086 1102 32-33 0.945563237487697 1102 34-35 0.9000126591173085 1102 36-37 0.9860656952365829 1102 38-39 0.9863623690810499 1102 40-41 1.0119066306502305 1102 42-43 1.0038947836772891 1102 44-45 0.9223190759148707 1102 46-47 0.7467392760105014 1102 48-49 0.7558211056932933 1102 50-51 0.6218780918017579 1102 52-53 0.781910484253455 1102 54-55 0.8824040499255545 1102 56-57 0.7715899669362365 1102 58-59 0.7629792564536757 1102 60-61 0.6767385404230808 1102 62-63 0.5727456573064238 1102 64-65 0.5151584430141263 1102 66-67 0.6259166123716327 1102 68-69 0.47772511095273273 1102 70-71 0.4288391020060196 1102 72-73 0.37175712684374673 1102 74-75 0.3471696048314712 1102 76-77 0.06466947570617876 1102 78-79 -0.119709980570601 1102 80-81 -0.035559012308041815 1102 82-83 -0.2702241497660225 1102 84-85 -0.3024344365497029 1102 86-87 -0.7139629046113853 1102 88-89 -1.434088964232938 1102 90-91 -5.671206108227295 1102 92-93 -10.943369866447828 1102 94-95 -9.346509871571325 1102 96-97 -5.95845666306143 1102 98-99 -4.7961487852213995 1102 100-101 -4.07010341255959 1103 1 0.7182026932345025 1103 2 0.8107560166085044 1103 3 0.7861158714041849 1103 4 0.80436476930916 1103 5 0.8083837946437953 1103 6 0.6166988047929607 1103 7 0.7525575984198198 1103 8 0.7537696960278097 1103 9 0.8117246349252767 1103 10-11 0.6319889069082123 1103 12-13 0.5816016810031428 1103 14-15 0.694265399827124 1103 16-17 0.7524597262324093 1103 18-19 0.8160975189548552 1103 20-21 0.7422490057743758 1103 22-23 0.8472994267950114 1103 24-25 0.6555226998843082 1103 26-27 0.7520991710687639 1103 28-29 0.8132164491959841 1103 30-31 0.8078426739729494 1103 32-33 1.0061616782247071 1103 34-35 0.9702957102317669 1103 36-37 0.9338055168794099 1103 38-39 0.8795925837893179 1103 40-41 0.7284969590188553 1103 42-43 0.8112376263550019 1103 44-45 0.8556821321416663 1103 46-47 0.9779864585278943 1103 48-49 0.6112395396336652 1103 50-51 0.6340817174003028 1103 52-53 0.8501930855614681 1103 54-55 0.9309701680066809 1103 56-57 0.7468594297345739 1103 58-59 0.6075121760443309 1103 60-61 0.557913248618572 1103 62-63 0.6877714716393974 1103 64-65 0.29750700734883395 1103 66-67 0.3385877790843672 1103 68-69 0.5556649775762033 1103 70-71 0.6834343108737464 1103 72-73 0.4098893204031917 1103 74-75 0.3081688092486843 1103 76-77 0.37543520679523823 1103 78-79 0.25056762134672184 1103 80-81 0.16016108918573124 1103 82-83 0.1418823810472034 1103 84-85 0.007329633029609539 1103 86-87 -0.15537878436444785 1103 88-89 -0.7068789432584843 1103 90-91 -2.21844073975754 1103 92-93 -9.27712833623401 1103 94-95 -5.791696793894506 1103 96-97 -3.2010784006023343 1103 98-99 -2.2561985413450607 1103 100-101 -1.702968808473484 1104 1 0.8816218766204074 1104 2 0.7290753342622622 1104 3 0.8253383598779216 1104 4 0.8708198623865471 1104 5 0.8594860616362467 1104 6 0.7070373011214741 1104 7 0.8454858011649975 1104 8 0.7928540620672493 1104 9 0.7844914949633477 1104 10-11 0.7184120605110706 1104 12-13 0.7277420869189228 1104 14-15 0.8959577030169257 1104 16-17 0.9350423266602661 1104 18-19 0.8354699041758238 1104 20-21 0.8800347889428437 1104 22-23 0.9466754845737384 1104 24-25 0.814883689112186 1104 26-27 0.6973568247290842 1104 28-29 0.8698230914931457 1104 30-31 1.0240354236515614 1104 32-33 0.9461469451650473 1104 34-35 0.7802619933677732 1104 36-37 0.7405822246544531 1104 38-39 0.7042913019988504 1104 40-41 0.6279943289821119 1104 42-43 0.7576071299208493 1104 44-45 0.7484145365213308 1104 46-47 0.375273512636916 1104 48-49 0.2880585621265652 1104 50-51 0.47968974520467 1104 52-53 0.5808721839610307 1104 54-55 0.6207520880298745 1104 56-57 0.6825338134122809 1104 58-59 0.42995846354885714 1104 60-61 0.6587811714498919 1104 62-63 0.4053512453755772 1104 64-65 0.4926042319801667 1104 66-67 0.45743115895104225 1104 68-69 0.4808737316119114 1104 70-71 0.47015899610092404 1104 72-73 0.4950488500302761 1104 74-75 0.22368641860989058 1104 76-77 0.19035203710308224 1104 78-79 -0.017101164624893528 1104 80-81 -0.3466233277484392 1104 82-83 -0.3185866748877402 1104 84-85 -0.20715059476176734 1104 86-87 -0.30471215624357484 1104 88-89 -0.44979008592004277 1104 90-91 -0.9779033756760818 1104 92-93 -4.241473296835213 1104 94-95 -2.124865326106299 1104 96-97 -1.3013496666389912 1104 98-99 -1.0754645330320152 1104 100-101 -0.856197893274949 1105 1 0.8852441519077594 1105 2 0.897336739590898 1105 3 0.8359190517513007 1105 4 0.8073092559356496 1105 5 0.7424019131807427 1105 6 0.7261574565110251 1105 7 0.8953246332370668 1105 8 0.8759658910273487 1105 9 1.056622671036699 1105 10-11 0.9230986358840525 1105 12-13 0.7760418174934856 1105 14-15 0.9001101268015574 1105 16-17 1.0015930197171627 1105 18-19 0.9630809638462452 1105 20-21 0.8819978700002196 1105 22-23 0.7607120623833836 1105 24-25 0.882510865575405 1105 26-27 0.9258285489822384 1105 28-29 0.9583638081508852 1105 30-31 1.0305853508997203 1105 32-33 1.1252838787276573 1105 34-35 1.1274996864733993 1105 36-37 1.1396545518079435 1105 38-39 1.0441077863798398 1105 40-41 0.9751904247499823 1105 42-43 1.1738716889890597 1105 44-45 0.8400324118894318 1105 46-47 1.0019539406543672 1105 48-49 1.0510045586873886 1105 50-51 0.904427107620176 1105 52-53 0.9461528528601413 1105 54-55 1.0455366173011669 1105 56-57 0.8345983641262258 1105 58-59 0.8852303604570722 1105 60-61 0.8726819922576112 1105 62-63 0.9699583506881986 1105 64-65 1.0265796843294588 1105 66-67 0.9117365704339981 1105 68-69 0.8150442911396176 1105 70-71 0.8344166141573055 1105 72-73 0.8989257567199616 1105 74-75 0.6793722003265543 1105 76-77 0.4121208464097492 1105 78-79 0.32180296308415635 1105 80-81 0.431601597237119 1105 82-83 0.4880875222314245 1105 84-85 0.2810900641122167 1105 86-87 -0.02530684153099827 1105 88-89 -0.22821238070070748 1105 90-91 -1.4205794594694794 1105 92-93 -7.230360019800424 1105 94-95 -3.0472065774377626 1105 96-97 -1.2162590923520646 1105 98-99 -0.7483456795173034 1105 100-101 -0.43630061607307624 1106 1 0.8052682178000268 1106 2 0.8825091508616367 1106 3 0.8025296378179512 1106 4 0.9174706949150817 1106 5 0.8740836728436747 1106 6 0.6875840006781573 1106 7 0.740007460869684 1106 8 0.7809260981842101 1106 9 0.8696453178459578 1106 10-11 0.9451011887705008 1106 12-13 0.8781110087624668 1106 14-15 0.7748356366473601 1106 16-17 0.9709287572014347 1106 18-19 1.121649043256376 1106 20-21 0.9729182989436609 1106 22-23 1.0017610500066425 1106 24-25 0.9181228999845032 1106 26-27 0.954146935616528 1106 28-29 0.8364823162118569 1106 30-31 0.9218845067647763 1106 32-33 0.8673483519113745 1106 34-35 0.857690128876186 1106 36-37 0.9988493319232177 1106 38-39 0.897932727129465 1106 40-41 0.8916570909289874 1106 42-43 0.8826129477303155 1106 44-45 0.6697475712071039 1106 46-47 0.9150113330527674 1106 48-49 0.9392245238686527 1106 50-51 0.9034280804966635 1106 52-53 0.9225434079117854 1106 54-55 0.96725754804406 1106 56-57 0.8473807177558577 1106 58-59 0.8770607580929166 1106 60-61 0.9837956432509678 1106 62-63 0.9064337215516467 1106 64-65 0.9404799315717582 1106 66-67 0.7099171816637728 1106 68-69 0.7667892512004784 1106 70-71 0.9931604330998702 1106 72-73 1.1392119872258597 1106 74-75 0.9035479983778743 1106 76-77 0.9053615492215812 1106 78-79 0.8535525680816711 1106 80-81 0.7654233194479616 1106 82-83 0.8126369268017513 1106 84-85 0.6305379749879485 1106 86-87 0.5375808460951816 1106 88-89 0.3368046629251218 1106 90-91 -0.06739255745935324 1106 92-93 -3.4268177984234747 1106 94-95 -1.0648593483070599 1106 96-97 -0.22291214118607527 1106 98-99 0.10549297659645873 1106 100-101 -0.08573956624424284 1107 1 0.5348508805547851 1107 2 0.6543478773083073 1107 3 0.681850418869896 1107 4 0.7254985886099803 1107 5 0.5158546055769264 1107 6 0.553922499246795 1107 7 0.5909948136391137 1107 8 0.46493724996521735 1107 9 0.6049724652403157 1107 10-11 0.30202431910248606 1107 12-13 0.46468552131712926 1107 14-15 0.6202044607967352 1107 16-17 0.7169054637942267 1107 18-19 0.6445763323623837 1107 20-21 0.5927994107035985 1107 22-23 0.5906697100838372 1107 24-25 0.6505084624871742 1107 26-27 0.6188459585894819 1107 28-29 0.4845676956999867 1107 30-31 0.7133176281240736 1107 32-33 0.4530570169734034 1107 34-35 0.43295904275252894 1107 36-37 0.4932029416536139 1107 38-39 0.49351937062853324 1107 40-41 0.509163019318315 1107 42-43 0.5427107928587134 1107 44-45 0.27936295582249215 1107 46-47 0.5396006185127291 1107 48-49 0.410374898443159 1107 50-51 0.3800174407291017 1107 52-53 0.49243573554342035 1107 54-55 0.43092123924084547 1107 56-57 0.30346345884369796 1107 58-59 0.30717430544169844 1107 60-61 0.3610397967553034 1107 62-63 0.27214030366607034 1107 64-65 0.37945998156250127 1107 66-67 0.2706902044658186 1107 68-69 0.15398838099125456 1107 70-71 0.19377374544943038 1107 72-73 0.33464570320438014 1107 74-75 0.3166733277328362 1107 76-77 0.12825136307371565 1107 78-79 0.01730313777673942 1107 80-81 0.05278499036360529 1107 82-83 0.10009188528522017 1107 84-85 0.08770963479453897 1107 86-87 0.037673243514461774 1107 88-89 -0.36662472337086527 1107 90-91 -0.6886456551763054 1107 92-93 -4.017821779167782 1107 94-95 -1.705351938710546 1107 96-97 -0.8061558959038635 1107 98-99 -0.7312113196164951 1107 100-101 -0.7455571926097306 1108 1 0.4446717455821485 1108 2 0.4321653652542743 1108 3 0.2615322965011231 1108 4 -0.12698265524912955 1108 5 -0.8159158244641276 1108 6 -0.10360045384496175 1108 7 0.17588627853763938 1108 8 0.22805125737006904 1108 9 0.46136988720630967 1108 10-11 0.2433980396831643 1108 12-13 0.33304116247457216 1108 14-15 0.30172478184720575 1108 16-17 0.3366107988020062 1108 18-19 0.33013434788784934 1108 20-21 0.43371528949305116 1108 22-23 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60-61 0.39545543055909604 2311 62-63 0.22870597730419462 2311 64-65 0.3807265366264545 2311 66-67 0.029099166691192835 2311 68-69 0.2100889494275613 2311 70-71 0.1381764647860244 2311 72-73 0.21779082540013306 2311 74-75 0.5207579409249554 2311 76-77 0.49482524942179396 2311 78-79 0.4416805966920627 2311 80-81 0.43898264394089637 2311 82-83 0.5510991906789506 2311 84-85 0.5496767977834125 2311 86-87 0.6434788381446275 2311 88-89 0.7849066128424234 2311 90-91 1.6218964989199485 2311 92-93 2.665623733074071 2311 94-95 1.866626049075638 2311 96-97 1.6652931441924466 2311 98-99 1.4864912573613367 2311 100-101 1.4491057751363705 2312 1 0.2371816401985214 2312 2 -0.006371185220192643 2312 3 -0.1840818849875987 2312 4 -0.2195283135391506 2312 5 -0.00556224094403035 2312 6 0.12195149885162238 2312 7 0.01617644114314487 2312 8 -0.5724947347142333 2312 9 -0.059234757302775165 2312 10-11 -0.010677826989855532 2312 12-13 0.04838616271299401 2312 14-15 -0.2962092699385934 2312 16-17 0.007443734761189091 2312 18-19 -0.07943839542434716 2312 20-21 0.11367213466018455 2312 22-23 0.09824562886061727 2312 24-25 0.2389884150664372 2312 26-27 0.022247864538357476 2312 28-29 -0.04209051021545207 2312 30-31 0.18484048735135872 2312 32-33 0.2406862080850587 2312 34-35 0.050974363298479375 2312 36-37 0.2915131979079817 2312 38-39 0.19740042361208765 2312 40-41 0.3841128627690864 2312 42-43 0.17051348003383993 2312 44-45 0.04540773194189285 2312 46-47 0.2497010836007334 2312 48-49 0.1649323048935969 2312 50-51 0.19968564755647122 2312 52-53 0.25499957115153293 2312 54-55 0.3574027573124141 2312 56-57 0.1558584340693976 2312 58-59 0.3365535041528247 2312 60-61 0.3865387024305953 2312 62-63 0.3357502073047023 2312 64-65 0.6264110713275244 2312 66-67 0.5546587274314341 2312 68-69 0.4095979350539345 2312 70-71 0.5804229839818227 2312 72-73 0.6396020822055064 2312 74-75 0.9880635760837535 2312 76-77 0.9460136210303673 2312 78-79 1.1340523854285003 2312 80-81 1.0644030710396528 2312 82-83 1.0486156868476755 2312 84-85 1.0206727469324228 2312 86-87 0.8624008022584206 2312 88-89 1.1997430305276673 2312 90-91 1.660951952508288 2312 92-93 2.83473240510434 2312 94-95 2.1047504262467456 2312 96-97 1.7440622090905116 2312 98-99 1.588803043395334 2312 100-101 1.486444965641784 2313 1 -0.1397720062036143 2313 2 -0.8118843621294367 2313 3 -0.33839650920575437 2313 4 -0.09463559310584202 2313 5 -0.6477730273666822 2313 6 -0.26874523918767323 2313 7 -1.1038509165252819 2313 8 -1.0322028460422956 2313 9 -1.1385060835981236 2313 10-11 -1.773581297442476 2313 12-13 -0.6197338800885177 2313 14-15 -0.17272924765533304 2313 16-17 -1.6730497236672903 2313 18-19 -1.0978293949720594 2313 20-21 -1.625690917043599 2313 22-23 -1.3158184741277594 2313 24-25 -0.4325543699000818 2313 26-27 -0.8650262692274069 2313 28-29 -2.437639649221083 2313 30-31 -0.8092347296898268 2313 32-33 -0.24882866213149413 2313 34-35 -0.07517096328368211 2313 36-37 -0.11797083977499767 2313 38-39 -0.5787501428460899 2313 40-41 -0.26595029152595373 2313 42-43 -0.18396275299172515 2313 44-45 -0.8167567781021248 2313 46-47 -0.9446346104346404 2313 48-49 -0.5921853772058867 2313 50-51 -0.44053867429325777 2313 52-53 -0.1445866165353138 2313 54-55 -0.08833294885740628 2313 56-57 -0.5497318241737545 2313 58-59 -0.3349858335268472 2313 60-61 -0.41951928369200786 2313 62-63 -0.46992899093911333 2313 64-65 -0.29878004625529186 2313 66-67 -0.43034626423991895 2313 68-69 -0.42606921147505616 2313 70-71 -0.4600454057767003 2313 72-73 -0.15591920272240145 2313 74-75 0.05517508064520271 2313 76-77 0.13546630219218514 2313 78-79 -0.05202940076249618 2313 80-81 -0.0177295033390088 2313 82-83 -0.31534789325624146 2313 84-85 0.06456456831332602 2313 86-87 0.3594688158490058 2313 88-89 0.4617726827102153 2313 90-91 1.0329915604552475 2313 92-93 2.279159659078374 2313 94-95 1.69604768165485 2313 96-97 1.143305736464729 2313 98-99 0.9150632335752533 2313 100-101 1.0581074404076425 2314 1 -0.3929873309990022 2314 2 -0.5100430433209908 2314 3 -1.6084784362336002 2314 4 -0.2582391628599936 2314 5 -0.19531220905221147 2314 6 -2.5879430085445065 2314 7 -3.4038094709907263 2314 8 -1.1142674763571918 2314 9 -1.7770346929644845 2314 10-11 -0.8983173190501859 2314 12-13 -1.843214917726506 2314 14-15 -2.733435832221936 2314 16-17 -1.778596174388717 2314 18-19 -2.212859292610652 2314 20-21 -2.0319611814792964 2314 22-23 -2.9987805279805926 2314 24-25 -1.683683204538987 2314 26-27 -1.8823429633733753 2314 28-29 -1.0740834163647435 2314 30-31 -1.3793141436186502 2314 32-33 -2.198618591772963 2314 34-35 -0.6940638236929857 2314 36-37 -0.3955376820290084 2314 38-39 -0.5020664283044738 2314 40-41 -0.513448522057061 2314 42-43 -0.711970005548288 2314 44-45 -1.1283293518698159 2314 46-47 -1.4778322364212357 2314 48-49 -1.5279165777833228 2314 50-51 -0.8581984270537539 2314 52-53 -0.6129874863147613 2314 54-55 -0.3800164038494529 2314 56-57 -0.3331304980814416 2314 58-59 -0.45857612917440704 2314 60-61 -0.34533280680139455 2314 62-63 -0.46335002730818786 2314 64-65 -0.6611963209197107 2314 66-67 -0.6632279401226562 2314 68-69 -0.5197530108852639 2314 70-71 -0.4742118685115635 2314 72-73 -0.39540044140830943 2314 74-75 -0.21019499172381018 2314 76-77 -0.22420121892814393 2314 78-79 0.12119027457807263 2314 80-81 -0.01510352086148714 2314 82-83 0.08340058343497248 2314 84-85 0.03556546561326712 2314 86-87 0.21824830665394757 2314 88-89 0.32633415517200604 2314 90-91 1.008687009109341 2314 92-93 1.9741547193968714 2314 94-95 1.2234660038769896 2314 96-97 1.1697530137073393 2314 98-99 1.1508683439826974 2314 100-101 1.0676716360800036 2315 1 -7.105631709410311 2315 2 -3.141646943972898 2315 3 -1.4386108202370025 2315 4 -1.149731387389231 2315 5 -1.5693057908524821 2315 6 -1.7962391464349565 2315 7 -3.1150780669853617 2315 8 -2.779324896174593 2315 9 -3.4414921506335183 2315 10-11 -2.4074631845884866 2315 12-13 -2.986219618788418 2315 14-15 -4.802909736009724 2315 16-17 -4.722155249869559 2315 18-19 -3.1383554736022248 2315 20-21 -4.347311053488291 2315 22-23 -5.597630473896967 2315 24-25 -3.523349985865707 2315 26-27 -4.546019765711883 2315 28-29 -2.9070255931650095 2315 30-31 -2.773233224017453 2315 32-33 -1.9245450242292108 2315 34-35 -2.6143172085818946 2315 36-37 -3.045899413754892 2315 38-39 -2.199005557634102 2315 40-41 -3.589860671048811 2315 42-43 -7.658098571233985 2315 44-45 -4.295915854586433 2315 46-47 -2.929934659616272 2315 48-49 -5.466989406253273 2315 50-51 -6.616406290522647 2315 52-53 -6.643210355053874 2315 54-55 -4.381510714362673 2315 56-57 -3.8465475194344556 2315 58-59 -4.2219946010460845 2315 60-61 -3.3012498177935647 2315 62-63 -3.586006714406448 2315 64-65 -2.4151913256097224 2315 66-67 -2.9835917025746674 2315 68-69 -2.2647965886102703 2315 70-71 -2.0006761671029025 2315 72-73 -2.4644843628924242 2315 74-75 -3.0563310862921007 2315 76-77 -3.048358847482092 2315 78-79 -2.1177258978761664 2315 80-81 -1.985546992767702 2315 82-83 -2.210118291957187 2315 84-85 -2.2396258992419114 2315 86-87 -2.7591653909391596 2315 88-89 -2.2236409787937568 2315 90-91 -1.2993369838508109 2315 92-93 -0.4624337159631864 2315 94-95 -0.6034065121097996 2315 96-97 -0.878163557984891 2315 98-99 -1.0977911695547853 2315 100-101 -1.0572755581333944 2316 1 -4.082496942571517 2316 2 -2.8629515055032755 2316 3 -4.4847362877458465 2316 4 -4.314747884377965 2316 5 -3.9842640964083493 2316 6 -4.2967880102896 2316 7 -2.881939740864752 2316 8 -1.3822245809239178 2316 9 -1.7060024386528596 2316 10-11 -2.9885682003510183 2316 12-13 -3.718129851441155 2316 14-15 -2.9462790199726143 2316 16-17 -2.822388298083702 2316 18-19 -2.9062479267948476 2316 20-21 -5.249164139894091 2316 22-23 -6.443489842717657 2316 24-25 -4.087944171242139 2316 26-27 -3.6318282594012175 2316 28-29 -4.227294996900564 2316 30-31 -5.931706715842399 2316 32-33 -4.3586809379477 2316 34-35 -5.956759384376518 2316 36-37 -9.107699704147091 2316 38-39 -5.854539534651309 2316 40-41 -5.249342202330947 2316 42-43 -4.369289405235861 2316 44-45 -3.270865007703346 2316 46-47 -5.655710498307361 2316 48-49 -3.0428286623068743 2316 50-51 -4.163734145575134 2316 52-53 -5.055301885093083 2316 54-55 -6.592084041217738 2316 56-57 -4.532946401932961 2316 58-59 -5.442294089187463 2316 60-61 -5.786475973430225 2316 62-63 -6.035668723875265 2316 64-65 -4.659504525460573 2316 66-67 -6.121219102265066 2316 68-69 -4.785089720891261 2316 70-71 -5.753903673006793 2316 72-73 -7.808321861903735 2316 74-75 -7.492588444966014 2316 76-77 -6.794511715811261 2316 78-79 -5.872877252098153 2316 80-81 -5.9328783788537365 2316 82-83 -4.782055614699303 2316 84-85 -6.198333286729056 2316 86-87 -4.829748632936422 2316 88-89 -4.804765203378789 2316 90-91 -5.2447278551404 2316 92-93 -3.8079313657072547 2316 94-95 -3.619975110204738 2316 96-97 -4.4485369153480825 2316 98-99 -5.68395361404694 2316 100-101 -4.827985352373007 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10487.0 3 3960.0 4 4183.0 5 4815.0 6 5526.0 7 6743.0 8 8330.0 9 10500.0 10 12644.0 11 15855.0 12 19520.0 13 22926.0 14 24872.0 15 28507.0 16 32167.0 17 36361.0 18 32301.0 19 29128.0 20 30677.0 21 33160.0 22 36991.0 23 41325.0 24 46327.0 25 52896.0 26 60167.0 27 68077.0 28 77286.0 29 88426.0 30 101849.0 31 117420.0 32 134063.0 33 157219.0 34 187163.0 35 223845.0 36 258626.0 37 233199.0 38 76944.0 39 1389.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.95423890036249 18.989828010249116 13.460875973708792 22.595057115679605 2 15.352522486619444 21.615265618505234 39.74948684655962 23.2827250483157 3 19.439476045556336 26.1627553202339 30.388732099837252 24.009036534372513 4 12.40281244000768 16.885567218824562 38.27325816581192 32.43836217535584 5 12.903759364989039 38.41586636111363 34.41075343275805 14.269620841139277 6 30.23538781943761 36.72334465693513 18.207520533935888 14.833746989691365 7 26.780485171229092 34.035215063669405 21.91390425979551 17.270395505305995 8 25.289358819504283 33.86315501268924 20.910446053695434 19.937040114111042 9 26.64892017162086 16.630162529102623 21.227712981505427 35.49320431777109 10-11 24.330074660744483 26.472974498714237 28.232019259382724 20.96493158115856 12-13 26.405863937338104 24.48927387842922 28.03612558344904 21.068736600783637 14-15 23.003819633102687 25.450887248407273 27.69476100940468 23.85053210908536 16-17 21.331760493584877 28.67297430823688 28.06505574854217 21.930209449636063 18-19 21.06557559687608 27.701528966026117 30.412699133663047 20.820196303434756 20-21 22.539121893689927 26.433376644970853 29.95312572317007 21.074375738169152 22-23 21.79958290367955 26.751508824081526 29.805715059611515 21.643193212627406 24-25 21.900762941552827 26.57244300979853 30.196024216079962 21.330769832568677 26-27 21.79148204919022 26.43695111780072 30.54695358925097 21.224613243758085 28-29 21.141730030259765 27.242790397385274 30.41885455314386 21.1966250192111 30-31 21.560404302996606 26.902581532165513 30.13490913814457 21.40210502669331 32-33 21.332408216563064 27.11378786232222 30.0030844370538 21.55071948406092 34-35 22.00393432615984 26.847253580566882 29.928849481803205 21.219962611470073 36-37 21.998820690262722 26.644619447550618 30.015254720829176 21.341305141357484 38-39 21.720110181973435 26.98429572526178 30.15811438966971 21.137479703095075 40-41 21.710102187778617 26.94456827378095 30.107438618750425 21.23789091969001 42-43 21.333149875094087 27.249870979135483 29.507778972256656 21.909200173513774 44-45 21.708910170950592 27.14775822227327 29.220184046568615 21.92314756020752 46-47 21.988609157171084 27.343124062533892 28.80785390296693 21.8604128773281 48-49 22.01589766225603 27.244345898384704 28.827659419844014 21.91209701951525 50-51 21.685094066570187 27.650168837433668 28.930653374068715 21.734083721927426 52-53 21.85479324990957 27.911396218420997 28.39239687475007 21.84141365691936 54-55 21.404239042293938 27.413401178135917 28.68501952624991 22.497340253320232 56-57 21.91848061750314 27.767921465590394 28.163795832655463 22.149802084251 58-59 22.21892396661127 27.417920230032966 28.189583446748077 22.173572356607682 60-61 21.930554195208067 27.297555468064672 28.847594335305875 21.924296001421382 62-63 21.92505678831156 27.335993277344063 28.727138136991904 22.011811797352472 64-65 21.707241811577898 27.762077721706145 28.46367308454673 22.06700738216922 66-67 21.56420287500702 27.549771689106272 28.271823911070836 22.61420152481587 68-69 21.451424552193433 27.633417221497975 28.451675080843174 22.46348314546542 70-71 21.803411922955252 27.514619281419773 28.092081969138547 22.589886826486424 72-73 21.6984317514642 27.17046167619573 28.395715470651428 22.73539110168865 74-75 21.571004065473833 27.88684341825709 28.046001755180043 22.496150761089034 76-77 21.739562076475476 27.120107228373406 28.251507228930905 22.88882346622021 78-79 21.44507385259756 27.329760151395305 28.43226910489841 22.79289689110872 80-81 21.569668904163873 27.649079755653638 28.08525018741112 22.69600115277137 82-83 21.620998618004116 27.449032870916867 28.17747014342094 22.75249836765808 84-85 21.5989001400765 27.27825830072242 28.04046825940264 23.08237329979844 86-87 21.563928717360984 27.255559228621788 28.15553191644446 23.02498013757277 88-89 21.718887096317683 26.996808537098932 28.015100482621925 23.269203883961463 90-91 21.326840193343457 26.935500174153326 28.487728896812172 23.249930735691045 92-93 21.026067207892744 27.235096790408992 28.740077420276677 22.998758581421587 94-95 21.493303485275387 27.10038553741514 28.11367564252662 23.29263533478285 96-97 21.309894431008935 26.882852882586533 28.196401600079945 23.610851086324583 98-99 21.583734059692087 26.912895935123622 27.714241406181127 23.789128599003163 100-101 21.589490402411432 26.679266960630628 28.119613127644367 23.611629509313577 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4666.0 1 3124.0 2 1639.5 3 1644.5 4 1383.5 5 1376.0 6 2010.5 7 3356.5 8 4893.5 9 5264.0 10 4366.5 11 3567.5 12 3462.5 13 3530.0 14 3646.5 15 3824.5 16 3921.0 17 4143.0 18 4508.5 19 4849.0 20 5373.5 21 6143.0 22 7227.5 23 8519.5 24 10155.5 25 12225.5 26 14709.5 27 17962.5 28 21515.0 29 25087.5 30 29059.0 31 33372.5 32 37536.5 33 41516.5 34 46361.0 35 51164.5 36 55612.0 37 59855.5 38 62617.5 39 63948.0 40 65243.5 41 67700.0 42 70115.5 43 72825.0 44 75854.5 45 77937.5 46 81665.0 47 91580.5 48 110764.0 49 111662.5 50 93987.0 51 84802.5 52 80286.0 53 77483.5 54 73859.5 55 68053.5 56 61521.0 57 54545.0 58 48060.0 59 42010.5 60 35423.0 61 28821.5 62 22730.0 63 17121.5 64 12452.0 65 9186.5 66 6686.0 67 4898.5 68 3559.0 69 2431.5 70 1700.5 71 1223.5 72 885.0 73 626.5 74 429.5 75 299.0 76 215.0 77 142.5 78 88.5 79 48.5 80 30.5 81 26.0 82 18.5 83 11.0 84 9.5 85 7.5 86 6.0 87 5.5 88 7.5 89 6.0 90 2.5 91 2.5 92 1.5 93 0.0 94 1.0 95 2.5 96 2.5 97 1.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08622040401151775 2 0.09692303608841915 3 0.09572434129580619 4 0.09598120446565182 5 0.09341257276719549 6 0.0775298667650738 7 0.08990210944597184 8 0.08592073031336452 9 0.08099751955798987 10-11 0.08778298829474536 12-13 0.08628461980397915 14-15 0.09170015163489127 16-17 0.08951681469120337 18-19 0.09161453057827607 20-21 0.10265964688163831 22-23 0.11130737359977465 24-25 0.133847116753729 26-27 0.138513464339258 28-29 0.13864189592418083 30-31 0.13986199598094762 32-33 0.1368010432069538 34-35 0.13155675348927212 36-37 0.13500300101803436 38-39 0.12868844809266256 40-41 0.12474987948836282 42-43 0.12492112160159324 44-45 0.11620917909099548 46-47 0.13369727990465238 48-49 0.15062884385031042 50-51 0.1604966706252135 52-53 0.16972233947550255 54-55 0.16631890247504788 56-57 0.13654418003710816 58-59 0.12804629016804844 60-61 0.12554187426205352 62-63 0.12275918992205916 64-65 0.10912403665608676 66-67 0.12290902677113578 68-69 0.1201905582236028 70-71 0.12826034280958648 72-73 0.1550811387943014 74-75 0.16779586570166027 76-77 0.17246221328718928 78-79 0.16976515000381012 80-81 0.1756087871177983 82-83 0.1755873818536445 84-85 0.15313325975630535 86-87 0.1533045018695358 88-89 0.1724408080230355 90-91 0.25816889095901574 92-93 0.5275327350704704 94-95 0.1739819870421093 96-97 0.18688936132685238 98-99 0.2052550779708152 100-101 0.21302518885864563 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335874.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.93451467242475 #Duplication Level Percentage of deduplicated Percentage of total 1 73.17382746234361 38.00247215980561 2 13.569481070137106 14.09448827468451 3 5.349294367486056 8.33439020445971 4 2.6316927727858963 5.467027476862532 5 1.5129160952607232 3.9286281573732795 6 0.9331601254694881 2.907793093675011 7 0.6146612801228633 2.234549468977855 8 0.43814178216378796 1.8203744651510059 9 0.3167345106971933 1.4804507783759993 >10 1.3651458610808618 12.000161280269726 >50 0.06142336530582575 2.181117423412774 >100 0.028905092571986053 2.6513823917112544 >500 0.0018464858298453345 0.6876639751753514 >1k 0.0024340040484324053 2.5572939969311914 >5k 2.5179352225162817E-4 1.0634032922399494 >10k+ 8.393117408387605E-5 0.5888035608942956 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13508 0.5782846163791369 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9237 0.3954408499773532 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8451 0.36179177472757523 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6708 0.2871730238874186 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4514 0.19324672478053184 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4278 0.18314344009993688 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3544 0.15172051232215436 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3497 0.1497084174916969 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3017 0.1291593639040462 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2990 0.12800347963974085 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2861 0.12248092148805971 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2460 0.10531389963670985 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.284315849228169E-4 0.0 18-19 0.0 0.0 0.0 1.498368490766197E-4 0.0 20-21 0.0 0.0 0.0 3.8529475476845065E-4 0.0 22-23 0.0 0.0 0.0 8.776158303059155E-4 0.0 24-25 0.0 2.1405264153802818E-5 0.0 0.0021619316795340846 0.0 26-27 0.0 4.2810528307605635E-5 0.0 0.003852947547684507 0.0 28-29 0.0 4.2810528307605635E-5 0.0 0.009097237265366197 0.0 30-31 0.0 4.2810528307605635E-5 0.0 0.022753795795492394 0.0 32-33 0.0 4.2810528307605635E-5 0.0 0.04970302336513014 0.0 34-35 0.0 4.2810528307605635E-5 0.0 0.09760800454134083 0.0 36-37 0.0 4.2810528307605635E-5 0.0 0.16263719704059382 0.0 38-39 0.0 8.562105661521127E-5 0.0 0.2530958433545645 0.0 40-41 0.0 8.562105661521127E-5 0.0 0.35295140063205466 0.0 42-43 0.0 8.562105661521127E-5 0.0 0.4551829422306169 0.0 44-45 0.0 8.562105661521127E-5 0.0 0.5794190953792884 0.0 46-47 0.0 8.562105661521127E-5 0.0 0.7238832231533037 0.0 48-49 0.0 8.562105661521127E-5 0.0 0.8735488301166929 0.0 50-51 0.0 8.562105661521127E-5 0.0 1.0335959901946765 0.0 52-53 0.0 8.562105661521127E-5 0.0 1.190303929064667 0.0 54-55 0.0 8.562105661521127E-5 0.0 1.3635367318613931 0.0 56-57 0.0 8.562105661521127E-5 0.0 1.5768187838898844 0.0 58-59 0.0 8.562105661521127E-5 0.0 1.7975284625797454 0.0 60-61 0.0 8.562105661521127E-5 0.0 2.0472208689338554 0.0 62-63 0.0 8.562105661521127E-5 0.0 2.2986042911561153 0.0 64-65 0.0 8.562105661521127E-5 0.0 2.5566233452660545 0.0 66-67 0.0 8.562105661521127E-5 0.0 2.849468764154231 0.0 68-69 0.0 8.562105661521127E-5 0.0 3.1760488793488006 0.0 70-71 0.0 8.562105661521127E-5 0.0 3.5309909695471586 0.0 72-73 0.0 8.562105661521127E-5 0.0 3.8935747390484248 0.0 74-75 0.0 8.562105661521127E-5 0.0 4.281737799213484 0.0 76-77 0.0 8.562105661521127E-5 0.0 4.684456439003132 2.1405264153802818E-5 78-79 0.0 8.562105661521127E-5 0.0 5.142871576120973 4.2810528307605635E-5 80-81 0.0 8.562105661521127E-5 0.0 5.634229414771516 4.2810528307605635E-5 82-83 2.1405264153802818E-5 8.562105661521127E-5 0.0 6.11449932659039 8.562105661521127E-5 84-85 4.2810528307605635E-5 8.562105661521127E-5 0.0 6.611229886543538 8.562105661521127E-5 86-87 4.2810528307605635E-5 8.562105661521127E-5 0.0 7.142615569161693 8.562105661521127E-5 88-89 4.2810528307605635E-5 8.562105661521127E-5 0.0 7.712787590426538 1.0702632076901408E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10285 0.0 25.655186 1 TACATGG 10600 0.0 24.579391 2 GAGTACT 9160 0.0 23.259962 12-13 ACATGGG 10755 0.0 23.033756 3 AGTACTT 9695 0.0 22.02535 12-13 GTACTTT 10530 0.0 20.166357 14-15 ATGGGAG 2860 0.0 20.078165 5 CATGGGA 7250 0.0 19.899412 4 CATGGGG 3930 0.0 18.838083 4 TAGCCCT 1490 0.0 18.473381 4 AGTACAT 7635 0.0 18.08792 2 TACTTTT 11895 0.0 18.051651 14-15 GTATCAA 27970 0.0 17.629004 1 ACTTTTT 13140 0.0 16.992592 16-17 GTGTAGC 1415 0.0 16.43404 1 AGAGTAC 20300 0.0 15.848631 10-11 GTATCGT 240 6.205739E-5 15.817126 6 TATCAAC 31325 0.0 15.756033 2 GTATATG 1090 0.0 15.67403 1 CAACGCA 31290 0.0 15.652322 5 >>END_MODULE