FastQCFastQC Report
Fri 27 May 2016
SRR939663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335874
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137170.5872320167954265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108750.46556449534521127No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88230.3777172912580045No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77100.3300691732516394No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50060.2143095047078738No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44300.18965064040269297No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40900.17509506077810702No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38050.16289406021043942No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34380.14718259632154818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33690.1442286698683234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.12406491103544112No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25190.10783972080685858No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25130.10758285763701296No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25020.1071119418256293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG102650.025.3999841
TACATGG104100.024.6356032
ACATGGG103350.023.2198033
GAGTACT107050.020.84797312-13
ATGGGAG25400.020.748335
CATGGGA69750.020.1709254
AGTACTT109850.019.73915512-13
CATGGGG37650.019.2466264
GTACTTT117950.019.00083514-15
TAGCCCT15050.018.4465164
GTATCAA247050.018.2690681
AGTACAT80000.017.5284582
GGTATCA197100.017.4786191
TAAGGCG3802.382876E-1017.3357755
TAGGACG3008.563802E-817.2532254
TACTTTT131550.016.62574414-15
ACTTTTT138200.016.47150616-17
GTATAGC10150.016.2287331
AACGCAG277850.016.1319036
ATCAACG278950.015.9574543
Produced by FastQC (version 0.11.5)