##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939663_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2335874 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.55287185867046 31.0 31.0 34.0 30.0 34.0 2 31.750875689356533 31.0 31.0 34.0 30.0 34.0 3 31.791858208105403 31.0 31.0 34.0 30.0 34.0 4 35.57583542605466 37.0 35.0 37.0 33.0 37.0 5 35.31872095840786 37.0 35.0 37.0 33.0 37.0 6 35.344150412222575 37.0 35.0 37.0 32.0 37.0 7 35.275563664820965 37.0 35.0 37.0 32.0 37.0 8 35.24986493278319 37.0 35.0 37.0 32.0 37.0 9 36.8460267976783 39.0 37.0 39.0 32.0 39.0 10-11 36.73347085502043 39.0 35.0 39.0 32.0 39.0 12-13 36.6789542158524 39.0 35.0 39.0 32.0 39.0 14-15 37.704780095159244 39.5 37.0 41.0 32.0 41.0 16-17 37.684206425517814 39.0 36.5 41.0 32.0 41.0 18-19 37.7006465245985 39.5 37.0 41.0 32.0 41.0 20-21 37.64778215777049 39.0 36.5 41.0 32.0 41.0 22-23 37.51094451156184 39.0 36.0 41.0 32.0 41.0 24-25 37.375786964536616 39.0 36.0 41.0 31.5 41.0 26-27 37.10094487117028 39.0 36.0 40.5 31.0 41.0 28-29 36.90405582664133 39.0 36.0 40.0 31.0 41.0 30-31 36.650711040064664 38.5 35.0 40.0 30.0 41.0 32-33 36.441536016069364 38.0 35.0 40.0 30.0 41.0 34-35 36.384378181357384 38.0 35.0 40.0 30.0 41.0 36-37 36.24010755717132 38.0 35.0 40.0 30.0 41.0 38-39 36.23399592615013 38.0 35.0 40.0 30.0 41.0 40-41 36.12827447028393 38.0 35.0 40.0 30.0 41.0 42-43 35.91763875106277 38.0 35.0 40.0 29.0 41.0 44-45 35.66176664494746 38.0 35.0 40.0 28.5 41.0 46-47 35.29660653785264 38.0 34.0 40.0 27.0 41.0 48-49 34.95492950390304 38.0 34.0 40.0 26.0 41.0 50-51 34.681531195603874 38.0 34.0 40.0 24.5 41.0 52-53 34.43967825319345 38.0 33.0 40.0 24.0 41.0 54-55 34.15967513658699 38.0 33.0 40.0 23.5 41.0 56-57 32.206891724468015 37.0 30.5 39.5 15.5 41.0 58-59 27.394498590249306 32.5 17.5 38.5 6.0 40.0 60-61 28.119901159052244 33.0 19.0 38.0 7.0 40.0 62-63 31.04493500077487 34.5 29.0 38.5 7.5 40.0 64-65 31.23552447606335 35.0 30.5 39.0 2.0 40.0 66-67 24.67949020366681 27.0 18.0 35.5 2.0 39.0 68-69 17.383439132418957 13.0 7.0 28.0 2.0 35.5 70-71 16.287072847251178 12.5 7.0 27.0 2.0 34.0 72-73 17.450012286621625 13.5 7.0 29.0 2.0 34.0 74-75 18.447259355598803 17.5 8.5 30.0 2.0 34.5 76-77 19.56621868302828 21.5 8.0 30.0 2.0 34.5 78-79 22.636488526350306 27.5 10.5 33.5 2.0 35.5 80-81 26.11717669703075 31.5 22.5 34.5 2.0 36.0 82-83 27.339734078122362 33.0 26.0 35.0 2.0 36.0 84-85 27.370380851021928 34.0 26.0 35.0 2.0 36.0 86-87 27.135405205931484 33.5 26.0 35.0 2.0 36.0 88-89 26.726062707149445 33.0 25.5 35.0 2.0 35.0 90-91 25.462461588253475 32.5 16.0 35.0 2.0 35.0 92-93 23.132304867471447 31.5 2.0 34.5 2.0 35.0 94-95 16.044611781286147 7.0 2.0 32.0 2.0 35.0 96-97 13.53635191795448 2.0 2.0 30.0 2.0 34.0 98-99 13.247345533192288 2.0 2.0 30.5 2.0 34.0 100-101 12.967688539707193 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4595619908604469 1101 2 0.3989721156491548 1101 3 0.4042329408967298 1101 4 0.22583195606996043 1101 5 0.38345767166075717 1101 6 0.3917143121039999 1101 7 0.4625853503798112 1101 8 0.3884692216430423 1101 9 0.4254647256295101 1101 10-11 0.5128412443373875 1101 12-13 0.47843769163302596 1101 14-15 0.663453158531091 1101 16-17 0.6795892800423573 1101 18-19 0.5088607511679513 1101 20-21 0.5192907825472659 1101 22-23 0.6700326386899462 1101 24-25 0.6007035509301843 1101 26-27 0.5825509450770667 1101 28-29 0.6971588889928526 1101 30-31 0.5120449409735031 1101 32-33 0.6938506614380131 1101 34-35 0.5093759721761018 1101 36-37 0.42477708753671095 1101 38-39 0.546166235892855 1101 40-41 0.41658236993232123 1101 42-43 0.4164641825665356 1101 44-45 0.34958264604083666 1101 46-47 0.5330129534739214 1101 48-49 0.5326024004650307 1101 50-51 0.4725299298686494 1101 52-53 0.3625820926724259 1101 54-55 0.2915386233491688 1101 56-57 -1.8638010175908164 1101 58-59 -9.706384942465299 1101 60-61 -9.918307130393522 1101 62-63 -3.52478799587643 1101 64-65 -4.575054891348525 1101 66-67 -9.361512224310713 1101 68-69 -4.450077243296914 1101 70-71 -4.181652955056224 1101 72-73 -4.279163356153177 1101 74-75 -6.407306883747172 1101 76-77 -7.758424600591159 1101 78-79 -11.077975307110908 1101 80-81 -7.740579834316801 1101 82-83 -3.9457016809112986 1101 84-85 -3.1329542519640228 1101 86-87 -2.7284609147941516 1101 88-89 -2.8031761237124186 1101 90-91 -9.433278708940083 1101 92-93 -16.80873772538917 1101 94-95 -10.962551563818096 1101 96-97 -9.237760907918032 1101 98-99 -9.19592122411555 1101 100-101 -9.064929253634512 1102 1 0.3659728009269756 1102 2 0.3302200424471273 1102 3 0.32182308215610433 1102 4 0.18284329061749105 1102 5 0.18296573602546573 1102 6 0.18400732048442592 1102 7 0.3478980048920377 1102 8 0.32131687806418086 1102 9 0.4091595002215982 1102 10-11 0.37250502573270694 1102 12-13 0.30929695172678606 1102 14-15 0.40509682439038386 1102 16-17 0.5059948327814894 1102 18-19 0.32968227116144533 1102 20-21 0.49836161251653976 1102 22-23 0.5012436390216592 1102 24-25 0.44979885250675267 1102 26-27 0.35619045514422254 1102 28-29 0.3394916402649457 1102 30-31 0.4213867110655727 1102 32-33 0.3972979661189058 1102 34-35 0.1743696821593872 1102 36-37 0.033364681495790194 1102 38-39 0.048539543690594655 1102 40-41 -0.01596987475871714 1102 42-43 0.05699191809792836 1102 44-45 0.08668122718831484 1102 46-47 0.024405957473248918 1102 48-49 -0.11238905637794261 1102 50-51 -0.10791781918158705 1102 52-53 -0.25227799357277547 1102 54-55 -0.508205394876228 1102 56-57 -3.329311698101776 1102 58-59 -10.333843838301373 1102 60-61 -10.651912092309193 1102 62-63 -4.752507880465576 1102 64-65 -5.622449071987791 1102 66-67 -6.744720070245986 1102 68-69 -4.54401879934065 1102 70-71 -4.231647009269283 1102 72-73 -4.472074100503114 1102 74-75 -5.224908916580432 1102 76-77 -7.440608519428038 1102 78-79 -10.141783614939945 1102 80-81 -5.857902728100008 1102 82-83 -3.5089429484278973 1102 84-85 -3.0401605457359686 1102 86-87 -2.9949479561079855 1102 88-89 -3.2540620459665597 1102 90-91 -9.121545480753298 1102 92-93 -13.540418129953034 1102 94-95 -10.483155906120178 1102 96-97 -9.117585914020287 1102 98-99 -9.249801945370798 1102 100-101 -9.107242602865002 1103 1 0.22376242823161618 1103 2 0.23216837811370894 1103 3 0.17774373391971565 1103 4 0.15761251711882096 1103 5 0.17005193142574626 1103 6 0.24366731039845746 1103 7 0.2004702736305859 1103 8 0.24710416320481698 1103 9 0.33547089905031413 1103 10-11 0.29656957196815625 1103 12-13 0.28115085776082793 1103 14-15 0.36102669025095935 1103 16-17 0.3888954503241351 1103 18-19 0.3141698930624628 1103 20-21 0.30688534575158855 1103 22-23 0.4424960428606681 1103 24-25 0.42036328034600956 1103 26-27 0.35006724186165883 1103 28-29 0.43529179541844343 1103 30-31 0.5506916015713799 1103 32-33 0.46480628605217333 1103 34-35 0.4931419681372162 1103 36-37 0.2170990262001098 1103 38-39 0.31122572046453456 1103 40-41 0.42562067653160085 1103 42-43 0.2216938024303019 1103 44-45 0.30731773304422205 1103 46-47 0.3993039831795926 1103 48-49 0.321832397012102 1103 50-51 0.3472251826126822 1103 52-53 0.25706054324844274 1103 54-55 0.2189548897898277 1103 56-57 -2.418419050257633 1103 58-59 -10.126838845846034 1103 60-61 -10.424752600253626 1103 62-63 -4.160578141422672 1103 64-65 -5.300972795315211 1103 66-67 -6.51222739941613 1103 68-69 -4.051248100565331 1103 70-71 -3.935028979163956 1103 72-73 -4.148138254396368 1103 74-75 -4.932238086727155 1103 76-77 -6.354944465403706 1103 78-79 -9.26210961563546 1103 80-81 -4.9608192374830296 1103 82-83 -2.799604015301437 1103 84-85 -2.2820538742343786 1103 86-87 -2.1372554235886625 1103 88-89 -2.215699797211702 1103 90-91 -5.066386032291309 1103 92-93 -10.15945603952212 1103 94-95 -8.375856855172167 1103 96-97 -7.850744972121609 1103 98-99 -8.079954037416655 1103 100-101 -8.068453335207373 1104 1 0.20111784402297062 1104 2 0.19657615408554463 1104 3 0.19249820790879468 1104 4 0.19637923724992845 1104 5 0.19652397037698677 1104 6 0.1630585937664506 1104 7 0.19042873696675144 1104 8 0.1985707236564025 1104 9 0.13637472657644167 1104 10-11 0.2303741276529152 1104 12-13 0.25354162861250273 1104 14-15 0.34744224176635186 1104 16-17 0.3822462113537668 1104 18-19 0.3067508981226652 1104 20-21 0.33975509451151 1104 22-23 0.35741128493896923 1104 24-25 0.3346448366824788 1104 26-27 0.3899950648593702 1104 28-29 0.40942435440957325 1104 30-31 0.334154884202718 1104 32-33 0.4520635992246227 1104 34-35 0.4211118324631613 1104 36-37 0.3527584555367511 1104 38-39 0.4079802180093779 1104 40-41 0.22518084402022254 1104 42-43 0.2927759078302117 1104 44-45 0.26034530899192987 1104 46-47 0.3093278351751536 1104 48-49 0.47117192551136355 1104 50-51 0.35054940057916184 1104 52-53 0.22308581501414437 1104 54-55 0.31735275877838376 1104 56-57 -2.7141998560218 1104 58-59 -9.96306159700223 1104 60-61 -10.44507033895739 1104 62-63 -4.2200622452079415 1104 64-65 -5.413704792822582 1104 66-67 -6.586375144736923 1104 68-69 -4.042407550959393 1104 70-71 -4.173973117026563 1104 72-73 -4.452640938682746 1104 74-75 -4.880732159597269 1104 76-77 -6.476578400265595 1104 78-79 -9.069102786605338 1104 80-81 -5.092851792976486 1104 82-83 -2.7441668631504754 1104 84-85 -2.300776512965335 1104 86-87 -2.1708439800557535 1104 88-89 -2.2673971149239946 1104 90-91 -4.646249976339945 1104 92-93 -6.3149795806529845 1104 94-95 -7.367632413711452 1104 96-97 -7.693493562166371 1104 98-99 -8.253508623616831 1104 100-101 -8.260442715840682 1105 1 0.26257817164987074 1105 2 0.28511582645864664 1105 3 0.29924150186099396 1105 4 0.16669440235133237 1105 5 0.22436320623911143 1105 6 0.1824979521579735 1105 7 0.2706757450912889 1105 8 0.1908492831005617 1105 9 0.30089759292889084 1105 10-11 0.4200670383526486 1105 12-13 0.3246283460081898 1105 14-15 0.48563399331015233 1105 16-17 0.3957785737344537 1105 18-19 0.35718427624420457 1105 20-21 0.4348048372694038 1105 22-23 0.4990554414702757 1105 24-25 0.45322875191441625 1105 26-27 0.42885272190237345 1105 28-29 0.4717855515774758 1105 30-31 0.5104858249639292 1105 32-33 0.5521372212910549 1105 34-35 0.7098011856332604 1105 36-37 0.4750911785437282 1105 38-39 0.5976833975079785 1105 40-41 0.5498306605742229 1105 42-43 0.6198568190033598 1105 44-45 0.652950903572787 1105 46-47 0.6660469365334194 1105 48-49 0.5923414894877155 1105 50-51 0.6111415795834816 1105 52-53 0.595523662673898 1105 54-55 0.6191661590387554 1105 56-57 1.7049095084265744 1105 58-59 -8.567900289836349 1105 60-61 -10.014474539766361 1105 62-63 -3.5242681015561494 1105 64-65 -4.615874816284176 1105 66-67 -6.122706677259426 1105 68-69 -3.993083899304896 1105 70-71 -4.016383256334132 1105 72-73 -2.176185728050534 1105 74-75 -4.42678789654893 1105 76-77 -6.250531504923799 1105 78-79 -8.878918714034516 1105 80-81 -4.139566125434943 1105 82-83 -1.863245421202258 1105 84-85 -1.3912935382857583 1105 86-87 -1.204169887365051 1105 88-89 -1.30660391112837 1105 90-91 -3.9537195702524635 1105 92-93 -6.3736638516378825 1105 94-95 -7.808392409255838 1105 96-97 -7.899309705414375 1105 98-99 -8.603194642038012 1105 100-101 -8.579180140913259 1106 1 0.20690147387066204 1106 2 0.2441142025595333 1106 3 0.1719409781169574 1106 4 0.1315121160184134 1106 5 0.0328173566911758 1106 6 0.28404285077400004 1106 7 0.20015902331933688 1106 8 0.06536979397044718 1106 9 0.11659199267140963 1106 10-11 0.29326319366177955 1106 12-13 0.27627144663141934 1106 14-15 0.3107689524932269 1106 16-17 0.31892553660421896 1106 18-19 0.2931754033179672 1106 20-21 0.30824256585880505 1106 22-23 0.2332050523196827 1106 24-25 0.4722323947151281 1106 26-27 0.33517166446608115 1106 28-29 0.332189661066316 1106 30-31 0.3488273997071829 1106 32-33 0.31970879720467593 1106 34-35 0.3071082196034709 1106 36-37 0.23476782511891514 1106 38-39 0.2151514681402773 1106 40-41 0.20850736566828942 1106 42-43 0.2734175104040091 1106 44-45 0.08089897537546875 1106 46-47 0.11857495245055816 1106 48-49 0.29466249484219986 1106 50-51 0.3417012395887369 1106 52-53 0.31695035313825315 1106 54-55 0.23742478325972627 1106 56-57 1.4791343160457373 1106 58-59 -8.569314069571256 1106 60-61 -10.112328725329753 1106 62-63 -3.942684361028892 1106 64-65 -5.152141896484313 1106 66-67 -6.481622212560946 1106 68-69 -3.994096824664057 1106 70-71 -3.9728550794900546 1106 72-73 -4.316044953553067 1106 74-75 -4.806290742029811 1106 76-77 -6.406703235912477 1106 78-79 -9.218277603053448 1106 80-81 -4.825371820785616 1106 82-83 -2.125327559774984 1106 84-85 -1.7133308367613402 1106 86-87 -1.4756102931935295 1106 88-89 -1.3339459467078498 1106 90-91 -3.7163633072685833 1106 92-93 -5.832450462516544 1106 94-95 -6.877881575946887 1106 96-97 -7.307394272520909 1106 98-99 -6.869079045291663 1106 100-101 -7.384717307721346 1107 1 0.08638408392862829 1107 2 -0.0138428353109461 1107 3 0.07256977905125694 1107 4 0.09280881321124212 1107 5 -0.022397311532657227 1107 6 -0.07163629696217555 1107 7 0.03330601888995233 1107 8 0.16401053713767055 1107 9 0.11284571349234085 1107 10-11 0.05480913244804242 1107 12-13 0.052195687868795915 1107 14-15 0.15674708332370102 1107 16-17 0.12017290176451922 1107 18-19 0.10656688708878193 1107 20-21 0.22952486044813014 1107 22-23 0.1569455811391265 1107 24-25 0.20085975004818835 1107 26-27 0.2218292278802636 1107 28-29 0.10458855799423361 1107 30-31 0.19054189702249147 1107 32-33 0.22864277310657855 1107 34-35 0.23553297712700783 1107 36-37 0.1314423191584737 1107 38-39 -0.01858133418977559 1107 40-41 0.11185334504532563 1107 42-43 0.12343077361737897 1107 44-45 -0.011976364661279604 1107 46-47 0.060040920291271505 1107 48-49 -0.1261908371618503 1107 50-51 -0.1087052022382693 1107 52-53 -0.06899883006143881 1107 54-55 -0.06386735637254048 1107 56-57 1.0819962326423749 1107 58-59 -8.690548118109277 1107 60-61 -10.431870512463188 1107 62-63 -4.140060737198549 1107 64-65 -5.374222565306322 1107 66-67 -6.7417737336467845 1107 68-69 -4.068100303364583 1107 70-71 -3.9604330565028594 1107 72-73 -2.494861643482281 1107 74-75 -4.5243971646296135 1107 76-77 -6.712448521685767 1107 78-79 -7.537666551681195 1107 80-81 -2.575161656713437 1107 82-83 -1.4665705454044264 1107 84-85 -1.3150150868289927 1107 86-87 -1.3104251600985215 1107 88-89 -1.138871761727863 1107 90-91 -0.5328962142801039 1107 92-93 -3.1533262262639994 1107 94-95 -5.84298196690013 1107 96-97 -6.465447891508845 1107 98-99 -6.229134786393738 1107 100-101 -6.7520806343132875 1108 1 -0.05558623809788088 1108 2 -0.12779005133354104 1108 3 -0.07939953440566327 1108 4 -0.013665390181451187 1108 5 -0.0230554845251163 1108 6 -0.008381577832537346 1108 7 -0.22950037479053975 1108 8 -0.08567664361752492 1108 9 -0.1657408901007713 1108 10-11 -0.17359682331804294 1108 12-13 -0.06382844947886213 1108 14-15 -0.18236231719554752 1108 16-17 -0.2298097736127218 1108 18-19 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13.159397579244347 2316 98-99 12.82339935396865 2316 100-101 11.89935965255242 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 33.0 5 125.0 6 283.0 7 643.0 8 1397.0 9 2373.0 10 4056.0 11 6723.0 12 9790.0 13 13454.0 14 16453.0 15 21894.0 16 29406.0 17 38939.0 18 41620.0 19 38987.0 20 39711.0 21 39696.0 22 39611.0 23 33556.0 24 34820.0 25 40578.0 26 56834.0 27 83280.0 28 117486.0 29 158964.0 30 197491.0 31 218038.0 32 220648.0 33 205429.0 34 187188.0 35 169312.0 36 138352.0 37 90672.0 38 35594.0 39 2427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.096897407405024 19.67023579361154 14.211805310227144 23.021061488756285 2 15.531151527606522 22.17469858884779 38.81477770927525 23.47937217427045 3 19.17834433352213 26.143760630412068 31.015737611997935 23.66215742406787 4 12.641734956594405 17.509077972527628 37.824471696675424 32.02471537420254 5 12.74122799496538 38.649466186068366 34.532942818633586 14.07636300033267 6 30.33181584280659 37.11129110559902 17.846467746119867 14.710425305474525 7 26.53439355033705 34.16010452618591 21.828703089293345 17.476798834183693 8 25.246774285385253 34.42621687539253 20.745265405706473 19.581743433515754 9 26.095457203599164 16.59271861410333 21.20225662856815 36.109567553729356 10-11 24.31723628928615 26.612715411875808 28.161942810271444 20.90810548856659 12-13 26.043057116950656 24.524118167332656 28.221278202505783 21.2115465132109 14-15 22.639420991952846 25.626440317194078 27.623458970305116 24.11067972054796 16-17 21.151162263033026 28.859240695345722 28.081887122336223 21.90770991928503 18-19 20.892065911202252 27.782689105129993 30.40216279159371 20.923082192074045 20-21 22.254988924916326 26.56964802039836 30.001125916894487 21.174237137790822 22-23 21.589811581818836 26.927463366936834 29.77235871205051 21.710366339193822 24-25 21.743065281574538 26.745326748216456 30.136242394975582 21.375365575233417 26-27 21.69027138739676 26.607566567881097 30.489186565590725 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21.774223782986372 27.913562687682138 28.389761427173703 21.922452102157784 54-55 21.372404407812873 27.374507507689554 28.686599346620977 22.566488737876597 56-57 20.94951722855486 27.735242121792446 29.096497900048597 22.218742749604093 58-59 20.601477728813972 27.205110986236974 30.154957976760656 22.038453308188398 60-61 21.69707044836765 27.327771493638625 29.09704415467833 21.878113903315395 62-63 21.839383345895232 27.427484725035654 28.688034527423866 22.045097401645243 64-65 21.260195210541532 27.781379452679246 28.84851583966615 22.109909497113076 66-67 21.034156330646166 27.402925640444835 28.958838786885288 22.604079242023708 68-69 21.10400407982727 27.191854802932134 29.313334128200285 22.390806989040314 70-71 21.06069733768773 27.228171646391946 29.086651095116807 22.62447992080352 72-73 20.326046859892973 27.058512651924882 29.78519141557081 22.830249072611334 74-75 20.85549266292719 27.51077355748362 29.049469344813478 22.584264434775715 76-77 19.31760970173702 27.46799192289013 29.850418021481502 23.363980353891346 78-79 19.936666191284957 27.444455968849535 29.42031522129019 23.198562618575323 80-81 21.05722253144805 27.647730409710782 28.27533153851475 23.019715520326418 82-83 21.31474095057638 27.469607644613326 28.110033662019283 23.105617742791008 84-85 21.333805392476577 27.26111584763763 27.9101686291441 23.49491013074169 86-87 21.179688192329774 27.333409536432573 28.02270509871512 23.464197172522532 88-89 21.35785751867291 27.04532912990486 27.810011768629344 23.786801582792886 90-91 20.64018581388098 27.009834668305526 28.4790157907036 23.870963727109896 92-93 19.672084648326138 27.430210922446456 29.177744767014467 23.719959662212936 94-95 18.878968225798243 27.464942092009302 29.314834986854038 24.341254695338417 96-97 18.740490848288182 27.584553155705578 28.735754234925054 24.939201761081183 98-99 19.176894124992483 27.313300084731612 28.56781861572899 24.94198717454691 100-101 19.54940753314022 27.01633754242132 28.602054807101172 24.832200117337287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1875.0 1 1380.0 2 901.5 3 964.0 4 1082.0 5 1247.5 6 1576.5 7 2196.5 8 2845.5 9 3209.5 10 3279.5 11 3375.5 12 3647.0 13 3923.0 14 4172.0 15 4332.5 16 4582.0 17 4849.0 18 5175.5 19 5601.0 20 6009.0 21 6798.0 22 7714.0 23 8968.5 24 10767.5 25 12831.0 26 15283.0 27 18321.0 28 21834.0 29 25729.0 30 30069.0 31 34545.0 32 39222.0 33 44277.0 34 49303.0 35 54254.0 36 58772.0 37 62638.5 38 65739.0 39 68237.5 40 70827.0 41 73720.0 42 76732.5 43 79761.5 44 83222.0 45 87269.0 46 92148.0 47 98209.0 48 103308.0 49 101389.0 50 94587.0 51 88657.0 52 82369.5 53 76729.5 54 71346.5 55 64166.5 56 56628.5 57 49207.0 58 42047.0 59 35033.0 60 28131.5 61 22011.5 62 16723.0 63 12261.0 64 8839.0 65 6337.5 66 4390.0 67 3077.5 68 2151.0 69 1410.5 70 933.0 71 639.0 72 406.0 73 246.0 74 164.0 75 104.5 76 56.0 77 17.5 78 8.0 79 6.0 80 3.0 81 3.5 82 3.0 83 2.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024359190607027605 2 0.008305242491675493 3 2.1405264153802816E-4 4 0.0 5 0.13810676432033578 6 0.0 7 0.0 8 0.0014983684907661971 9 0.0 10-11 0.0 12-13 0.0 14-15 7.277789812292957E-4 16-17 0.0 18-19 1.7124211323042254E-4 20-21 0.0 22-23 2.568631698456338E-4 24-25 2.1405264153802818E-5 26-27 2.782684339994366E-4 28-29 0.0030395475098399997 30-31 0.012457863737513238 32-33 0.002782684339994366 34-35 0.0044737002081447885 36-37 0.1033660205987138 38-39 0.10270245740994591 40-41 0.11543858958145858 42-43 0.11826408444976055 44-45 0.11413286846807662 46-47 0.10739021025962872 48-49 0.10749723658039774 50-51 0.1064697839010152 52-53 0.08452938814336733 54-55 0.07279930338708338 56-57 0.5447639727142817 58-59 1.5698620730398984 60-61 0.5189706294089493 62-63 0.004602131793067605 64-65 0.12220265305406028 66-67 1.9099917204438253 68-69 2.874748381119872 70-71 2.785509834862668 72-73 2.6318628487666715 74-75 2.0143423831936142 76-77 1.4051485653763858 78-79 0.9394342331820981 80-81 0.0509659339502045 82-83 2.9967369815323946E-4 84-85 3.6388949061464786E-4 86-87 4.7091581138366195E-4 88-89 1.284315849228169E-4 90-91 0.24832246944826644 92-93 0.38630080218367946 94-95 2.002184193154254 96-97 2.4324728131739985 98-99 1.4007390809607025 100-101 0.9431801544090134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2335874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.88680463256195 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22820889924833 32.45889505721243 2 15.077891493959438 14.139083054964875 3 6.102134848973905 8.58328813516162 4 3.0743460930678186 5.7658505855420366 5 1.8008241133264344 4.221744418957156 6 1.1493427311588027 3.2333404855018766 7 0.746195744512883 2.4490713863437255 8 0.5349711833579772 2.006647148652476 9 0.40354530239340264 1.702885477833684 >10 1.7527556130673212 13.887459935949057 >50 0.08586292188799673 2.77077917003138 >100 0.038332604497965016 3.255844522772301 >500 0.0022903485851340022 0.7667870391433754 >1k 0.00284003224556597 2.776773366107326 >5k 2.748418302160616E-4 0.9252045283798295 >10k+ 1.8322788681070774E-4 1.0563456874468065 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13717 0.5872320167954265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10875 0.46556449534521127 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8823 0.3777172912580045 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7710 0.3300691732516394 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5006 0.2143095047078738 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4430 0.18965064040269297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4090 0.17509506077810702 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3805 0.16289406021043942 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3438 0.14718259632154818 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3369 0.1442286698683234 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2898 0.12406491103544112 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2519 0.10783972080685858 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2513 0.10758285763701296 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2502 0.1071119418256293 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.284315849228169E-4 0.0 18-19 0.0 0.0 0.0 1.7124211323042254E-4 0.0 20-21 0.0 0.0 0.0 4.281052830760563E-4 0.0 22-23 0.0 0.0 0.0 0.0010060474152287324 0.0 24-25 0.0 0.0 0.0 0.002333173792764507 0.0 26-27 0.0 0.0 0.0 0.004045594925068732 0.0 28-29 0.0 0.0 0.0 0.009439721491827043 0.0 30-31 0.0 0.0 0.0 0.02395249058810535 0.0 32-33 0.0 0.0 0.0 0.05207900768620225 0.0 34-35 0.0 2.1405264153802818E-5 0.0 0.10154657314564056 0.0 36-37 0.0 4.2810528307605635E-5 0.0 0.16884472364519662 0.0 38-39 0.0 4.2810528307605635E-5 0.0 0.2626639964313144 0.0 40-41 0.0 4.2810528307605635E-5 0.0 0.365066780143107 0.0 42-43 0.0 4.2810528307605635E-5 0.0 0.47001679028920224 0.0 44-45 0.0 4.2810528307605635E-5 0.0 0.5973353014760214 0.0 46-47 0.0 4.2810528307605635E-5 0.0 0.7407505713065002 0.0 48-49 0.0 4.2810528307605635E-5 0.0 0.8675125456253205 0.0 50-51 4.2810528307605635E-5 4.2810528307605635E-5 0.0 0.9903573566039949 0.0 52-53 4.2810528307605635E-5 4.2810528307605635E-5 0.0 1.1103338621860597 0.0 54-55 4.2810528307605635E-5 4.2810528307605635E-5 0.0 1.2413982945997943 0.0 56-57 4.2810528307605635E-5 4.2810528307605635E-5 0.0 1.356943910502022 2.1405264153802818E-5 58-59 4.2810528307605635E-5 4.2810528307605635E-5 0.0 1.4337031877575588 4.2810528307605635E-5 60-61 4.2810528307605635E-5 4.2810528307605635E-5 0.0 1.49607812750174 4.2810528307605635E-5 62-63 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.5512608984902438 4.2810528307605635E-5 64-65 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.6063366431579786 4.2810528307605635E-5 66-67 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.6534710348246522 4.2810528307605635E-5 68-69 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.719613301059903 4.2810528307605635E-5 70-71 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.8199825846770845 4.2810528307605635E-5 72-73 8.562105661521127E-5 4.2810528307605635E-5 0.0 1.9595020964315712 4.2810528307605635E-5 74-75 8.562105661521127E-5 4.2810528307605635E-5 0.0 2.1483821473247273 4.2810528307605635E-5 76-77 8.562105661521127E-5 4.2810528307605635E-5 0.0 2.4337357237590727 4.2810528307605635E-5 78-79 8.562105661521127E-5 4.2810528307605635E-5 0.0 2.8650303911940456 4.2810528307605635E-5 80-81 8.562105661521127E-5 4.2810528307605635E-5 0.0 3.3742830306771685 4.2810528307605635E-5 82-83 8.562105661521127E-5 4.2810528307605635E-5 0.0 3.869301169498012 4.2810528307605635E-5 84-85 8.562105661521127E-5 4.2810528307605635E-5 0.0 4.270414414476123 6.421579246140846E-5 86-87 8.562105661521127E-5 8.562105661521127E-5 0.0 4.578821460404114 8.562105661521127E-5 88-89 8.562105661521127E-5 8.562105661521127E-5 0.0 4.874856263651207 8.562105661521127E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10265 0.0 25.399984 1 TACATGG 10410 0.0 24.635603 2 ACATGGG 10335 0.0 23.219803 3 GAGTACT 10705 0.0 20.847973 12-13 ATGGGAG 2540 0.0 20.74833 5 CATGGGA 6975 0.0 20.170925 4 AGTACTT 10985 0.0 19.739155 12-13 CATGGGG 3765 0.0 19.246626 4 GTACTTT 11795 0.0 19.000835 14-15 TAGCCCT 1505 0.0 18.446516 4 GTATCAA 24705 0.0 18.269068 1 AGTACAT 8000 0.0 17.528458 2 GGTATCA 19710 0.0 17.478619 1 TAAGGCG 380 2.382876E-10 17.335775 5 TAGGACG 300 8.563802E-8 17.253225 4 TACTTTT 13155 0.0 16.625744 14-15 ACTTTTT 13820 0.0 16.471506 16-17 GTATAGC 1015 0.0 16.228733 1 AACGCAG 27785 0.0 16.131903 6 ATCAACG 27895 0.0 15.957454 3 >>END_MODULE