FastQCFastQC Report
Fri 27 May 2016
SRR939662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381072
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.2449736925216877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.17702110645961147No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38090.1599699631090534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.12103791905494668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG87050.032.4213941
TACATGG106000.026.562762
GTATAGC9650.026.0199721
ACATGGG104900.025.7164923
GAGTACT95650.024.13136312-13
GTGTAGC11800.022.9813691
GTACCGT1252.3562179E-422.6554056
CATGGGA71100.022.1903384
GTACTTT108400.021.44859714-15
AGTACTT103200.021.19961512-13
CATGGGG39550.021.140434
TAGCCCT13900.021.0699734
GTATCAA262150.019.2713571
TGTACCG2302.1893484E-618.468695
ATGGGAG28950.017.7704895
GTAGCCC9100.017.6517853
GTCCTAC5704.7926915E-717.6205431
ACTTTTT138050.017.61376416-17
CCTATAC5700.017.405863
AGTACAT73250.017.2846262
Produced by FastQC (version 0.11.5)