##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939662_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381072 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.348445154115458 2.0 2.0 31.0 2.0 33.0 2 22.650278110027752 16.0 16.0 31.0 16.0 34.0 3 27.136035785562132 28.0 25.0 31.0 16.0 34.0 4 32.39460083525404 35.0 32.0 35.0 26.0 37.0 5 33.02030807972208 35.0 33.0 37.0 28.0 37.0 6 33.422436616784374 35.0 33.0 37.0 28.0 37.0 7 33.59578920755021 35.0 33.0 37.0 28.0 37.0 8 33.53769226634054 35.0 33.0 37.0 28.0 37.0 9 34.91089853645753 37.0 34.0 39.0 28.0 39.0 10-11 34.754274545246844 37.0 34.0 39.0 27.0 39.0 12-13 34.786261608216805 37.0 34.0 39.0 27.0 39.0 14-15 35.452668167951245 38.0 34.0 40.0 27.0 41.0 16-17 35.08282424890973 38.0 33.0 40.0 26.0 41.0 18-19 35.37053205446958 38.0 34.0 40.0 27.0 41.0 20-21 35.376723593406666 38.0 34.0 40.0 27.0 41.0 22-23 35.20138387247425 38.0 34.0 40.0 26.0 41.0 24-25 34.9905469049235 38.0 34.0 40.0 25.0 41.0 26-27 35.068295918813035 38.0 34.0 40.0 25.0 41.0 28-29 34.807259083303656 38.0 33.5 40.0 24.5 41.0 30-31 34.667930243184585 38.0 33.0 40.0 24.0 41.0 32-33 34.32831136563699 38.0 33.0 40.0 24.0 41.0 34-35 34.03937491180443 38.0 33.0 40.0 23.0 41.0 36-37 33.73282118306376 38.0 32.5 40.0 21.0 41.0 38-39 33.51238916756822 37.0 32.0 40.0 19.0 41.0 40-41 33.117258108952605 37.0 31.0 40.0 17.0 41.0 42-43 32.84862763494762 37.0 31.0 40.0 16.0 41.0 44-45 32.47161803590988 37.0 30.5 40.0 14.5 41.0 46-47 32.081642638273856 37.0 30.0 39.5 11.0 41.0 48-49 31.667739992742764 36.0 30.0 39.0 8.5 40.0 50-51 30.972658113656372 35.5 29.0 38.5 8.0 40.0 52-53 30.99631804498142 35.5 29.0 39.0 7.0 40.0 54-55 31.131318372564962 36.0 29.0 39.0 4.0 40.0 56-57 30.859614702957323 36.0 28.5 39.0 2.0 40.0 58-59 30.468137250784523 35.0 28.0 39.0 2.0 40.0 60-61 29.868511745969883 35.0 27.5 38.0 2.0 40.0 62-63 29.490401172245107 34.0 27.0 38.0 2.0 40.0 64-65 28.925648825403012 34.0 26.0 38.0 2.0 40.0 66-67 28.332553782497968 33.5 26.0 37.0 2.0 39.0 68-69 28.01103515559378 33.0 25.5 37.0 2.0 39.0 70-71 27.484066420503034 33.0 24.5 36.0 2.0 39.0 72-73 26.8946970104222 32.0 23.0 36.0 2.0 38.5 74-75 26.356519038483505 32.0 22.0 35.0 2.0 38.0 76-77 25.72989645000235 31.5 20.5 35.0 2.0 37.0 78-79 25.080498825738996 31.0 18.5 34.5 2.0 36.0 80-81 24.347652233951763 30.5 15.0 34.0 2.0 36.0 82-83 23.663084736622835 30.0 7.5 34.0 2.0 35.0 84-85 22.98194468709892 29.5 4.5 34.0 2.0 35.0 86-87 22.31429121000961 29.0 2.0 34.0 2.0 35.0 88-89 21.52127634107662 29.0 2.0 33.5 2.0 35.0 90-91 20.563201784742333 27.0 2.0 33.0 2.0 34.0 92-93 19.53169979740218 25.5 2.0 32.0 2.0 34.0 94-95 18.53993831349913 25.0 2.0 32.0 2.0 34.0 96-97 17.623408699946914 23.5 2.0 32.0 2.0 34.0 98-99 16.579857517958295 19.0 2.0 31.0 2.0 34.0 100-101 15.007183739088948 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 15.030652960265616 1101 2 8.158485610704808 1101 3 3.8505035936113075 1101 4 2.4507916817556605 1101 5 1.7367278525722298 1101 6 1.4894804768315026 1101 7 0.974974180386269 1101 8 1.0824031845375472 1101 9 1.0392674623014742 1101 10-11 1.0914870240735084 1101 12-13 0.8845047867466604 1101 14-15 0.9311238338856 1101 16-17 1.1929167519313637 1101 18-19 1.0852167913571265 1101 20-21 1.0777789870870436 1101 22-23 0.97978782128164 1101 24-25 1.2964138910920155 1101 26-27 1.3654489514339616 1101 28-29 1.2647356098988425 1101 30-31 1.254421547530427 1101 32-33 1.20451336213479 1101 34-35 1.19825031965852 1101 36-37 1.286842985960547 1101 38-39 1.321081764792062 1101 40-41 1.2374336065444496 1101 42-43 1.1418867765996623 1101 44-45 1.0030739608344916 1101 46-47 0.7457733232603161 1101 48-49 0.8199495439972146 1101 50-51 0.838122422879163 1101 52-53 0.8680305719645993 1101 54-55 0.6629192196918261 1101 56-57 0.6106954940643767 1101 58-59 0.5696273038997219 1101 60-61 0.6159212093839237 1101 62-63 0.39029746426610146 1101 64-65 0.5306522676237293 1101 66-67 0.38337997154640036 1101 68-69 0.2823411674088341 1101 70-71 0.14170547570097014 1101 72-73 0.3490569488775712 1101 74-75 0.2636056139474512 1101 76-77 0.2287252659047887 1101 78-79 0.16329405392398044 1101 80-81 -0.02910097738356754 1101 82-83 -0.10061103528812865 1101 84-85 -0.1256564016977748 1101 86-87 -0.17054732649574333 1101 88-89 -0.22599671417413347 1101 90-91 -0.20154378902426018 1101 92-93 0.039000621943632297 1101 94-95 -0.12765404380479595 1101 96-97 -0.8093727275617653 1101 98-99 -0.974934979951529 1101 100-101 -0.9284836556854881 1102 1 14.887671698064153 1102 2 7.988078378315965 1102 3 3.6581714948684017 1102 4 2.11828169252032 1102 5 1.4959095563070193 1102 6 1.195365276773117 1102 7 0.9217225246363014 1102 8 0.872993598520651 1102 9 0.9002649262216309 1102 10-11 0.9852979857768744 1102 12-13 0.611873375846983 1102 14-15 0.6762363154123605 1102 16-17 0.8745602776297048 1102 18-19 0.8245069626793509 1102 20-21 0.785578892212115 1102 22-23 0.8418755258557056 1102 24-25 0.773873706085908 1102 26-27 0.9214757900918471 1102 28-29 0.9379374563109266 1102 30-31 0.998761528190542 1102 32-33 0.9684190163156785 1102 34-35 1.1484002950275212 1102 36-37 1.1252919633182756 1102 38-39 1.0892917303086804 1102 40-41 1.0512802375722075 1102 42-43 0.9241614942555216 1102 44-45 1.099367635231033 1102 46-47 0.9580762297172853 1102 48-49 0.7392615183080089 1102 50-51 0.7290186260574743 1102 52-53 0.9451435461111863 1102 54-55 0.6530871665618676 1102 56-57 0.6622880244882126 1102 58-59 0.49672294512652826 1102 60-61 0.6991303538674956 1102 62-63 0.284480262711611 1102 64-65 0.47026172957341217 1102 66-67 0.46299636666399024 1102 68-69 0.28871721878725864 1102 70-71 0.3504770278183251 1102 72-73 0.3477953859955605 1102 74-75 0.3921168985904835 1102 76-77 0.18963250741622062 1102 78-79 0.02888793449104199 1102 80-81 0.12463435805404188 1102 82-83 0.06294047997396035 1102 84-85 -0.10144349870738978 1102 86-87 -0.28127339338256974 1102 88-89 -0.4069118923383037 1102 90-91 -0.16275481276123926 1102 92-93 0.21634385024555414 1102 94-95 0.31284268118562863 1102 96-97 -0.4572287366113734 1102 98-99 -0.8739769256173879 1102 100-101 -0.8783625076988439 1103 1 14.88856843509399 1103 2 7.9052426187139595 1103 3 3.7687115455563855 1103 4 2.2011284952568104 1103 5 1.6403133788880169 1103 6 1.137102044337226 1103 7 0.7649005247798613 1103 8 0.8722680300753041 1103 9 0.9138855973129125 1103 10-11 0.8888901276204209 1103 12-13 0.49837385139196044 1103 14-15 0.8167606330846908 1103 16-17 0.76771294758354 1103 18-19 0.8156590825470573 1103 20-21 0.7104166444096904 1103 22-23 0.7762187423342723 1103 24-25 1.0669352011606748 1103 26-27 1.0248203759190844 1103 28-29 1.0718737677935835 1103 30-31 1.0132105206425592 1103 32-33 0.9875622036679772 1103 34-35 0.922114736134489 1103 36-37 0.9357667559834226 1103 38-39 0.9519327373321076 1103 40-41 0.8813745390387382 1103 42-43 0.9223104751122477 1103 44-45 0.6957323561434166 1103 46-47 0.6486702054113493 1103 48-49 0.6897879307249006 1103 50-51 0.577789542444382 1103 52-53 0.6617491074337103 1103 54-55 0.6419749262135674 1103 56-57 0.530218520380167 1103 58-59 0.42516291602786893 1103 60-61 0.6542675337546342 1103 62-63 0.36531462673749715 1103 64-65 0.4003108059532501 1103 66-67 0.3675633239491489 1103 68-69 0.2976150518481937 1103 70-71 0.26317000659342327 1103 72-73 0.33745297290503373 1103 74-75 0.36067726726552607 1103 76-77 0.28460984828465286 1103 78-79 0.07494022097203512 1103 80-81 -0.0025381198320779674 1103 82-83 -0.03062247693572928 1103 84-85 -0.12053733705246472 1103 86-87 -0.18235796722801112 1103 88-89 -0.2636669086821435 1103 90-91 -0.339863654584903 1103 92-93 0.059569128808622196 1103 94-95 -0.19236657240891475 1103 96-97 -0.8159491720697751 1103 98-99 -1.1917159439103386 1103 100-101 -1.2345859869211875 1104 1 14.840660891038777 1104 2 7.944272784001015 1104 3 3.7581194269593254 1104 4 2.1707920342344735 1104 5 1.5556861014575176 1104 6 1.0821940349288184 1104 7 1.015723726569803 1104 8 1.0354711600755167 1104 9 1.017556353931866 1104 10-11 0.7209765466409621 1104 12-13 0.5030152994270836 1104 14-15 0.8173019061790043 1104 16-17 0.9017921682969572 1104 18-19 0.654681464168668 1104 20-21 0.8002273930016344 1104 22-23 0.7126191954202667 1104 24-25 0.8145075975829172 1104 26-27 0.8658274255532064 1104 28-29 0.8789472029268168 1104 30-31 0.756835498641621 1104 32-33 0.8508774551187059 1104 34-35 0.9380280817705682 1104 36-37 0.8743449686538298 1104 38-39 0.6203482563942586 1104 40-41 0.7916515441909482 1104 42-43 0.6111427819573336 1104 44-45 0.49083836399974956 1104 46-47 0.281389368095617 1104 48-49 0.3756135259502962 1104 50-51 0.3551712940657872 1104 52-53 0.4671434709265512 1104 54-55 0.6149668572026243 1104 56-57 0.35484020423867335 1104 58-59 0.4098674300871359 1104 60-61 0.14285746359164264 1104 62-63 0.29571832396191056 1104 64-65 0.48711575963127274 1104 66-67 0.5060435657727993 1104 68-69 0.5000018536850739 1104 70-71 0.348133631708091 1104 72-73 0.290737479710657 1104 74-75 0.14878872668779763 1104 76-77 -0.023360791795994373 1104 78-79 -0.16846049745861436 1104 80-81 -0.2053153549941129 1104 82-83 -0.35686039642029144 1104 84-85 -0.3946124475905144 1104 86-87 -0.2779293782748802 1104 88-89 -0.3798135309380264 1104 90-91 -0.3256909665502121 1104 92-93 0.1800757704253293 1104 94-95 0.1809863573160868 1104 96-97 -0.47201808100988885 1104 98-99 -0.9276219258989045 1104 100-101 -0.9856661163400418 1105 1 14.875097719171759 1105 2 8.091462226546604 1105 3 3.8425299848095236 1105 4 2.276011195676489 1105 5 1.7226432309286182 1105 6 1.2528766631972275 1105 7 0.9520248047892821 1105 8 0.9326211064960148 1105 9 1.0193796072054226 1105 10-11 0.9175741574799545 1105 12-13 0.8732455445207421 1105 14-15 1.1171467581435692 1105 16-17 1.123572640423241 1105 18-19 1.1162004472102325 1105 20-21 1.076203475005812 1105 22-23 0.9029896113548759 1105 24-25 1.078783261736234 1105 26-27 1.133856458420908 1105 28-29 1.3089748287759804 1105 30-31 1.0215400956607539 1105 32-33 0.886908557946164 1105 34-35 1.0426275086067633 1105 36-37 0.86047655160246 1105 38-39 0.835839609628799 1105 40-41 1.062328713913466 1105 42-43 1.0013884923813166 1105 44-45 1.0681279106699932 1105 46-47 0.8532194618671696 1105 48-49 0.6512691039080671 1105 50-51 0.5184011931766825 1105 52-53 0.844268057371611 1105 54-55 0.8228670387033965 1105 56-57 0.871098260541185 1105 58-59 0.6936554993219453 1105 60-61 0.6975156481287534 1105 62-63 0.5238169392722902 1105 64-65 0.608701406195209 1105 66-67 0.7040378057700529 1105 68-69 0.6331316651623986 1105 70-71 0.6766854877291308 1105 72-73 0.5109282121881478 1105 74-75 0.46168906815200117 1105 76-77 0.45404082822127023 1105 78-79 0.46975936683302777 1105 80-81 0.4904638862383237 1105 82-83 0.5398348910570796 1105 84-85 0.5430599459868048 1105 86-87 0.5030931376497243 1105 88-89 0.42333324655746196 1105 90-91 0.6133394670694159 1105 92-93 0.7998636424268817 1105 94-95 1.0262676670240864 1105 96-97 0.6411289174660091 1105 98-99 0.3864256172112377 1105 100-101 0.33817605351605273 1106 1 14.843754594162043 1106 2 7.943891315495723 1106 3 3.5692636323817304 1106 4 2.253139369376818 1106 5 1.6042915468397467 1106 6 1.1957730550646772 1106 7 0.9969577029561947 1106 8 1.0307049015134595 1106 9 0.9871392429716437 1106 10-11 0.9864285222734495 1106 12-13 0.6602088853343062 1106 14-15 0.9802823079925034 1106 16-17 1.0571735367555206 1106 18-19 0.9713342103546267 1106 20-21 0.8707902720496534 1106 22-23 1.061284636568267 1106 24-25 0.9910808808455513 1106 26-27 0.839522961403496 1106 28-29 0.9063996226377 1106 30-31 0.9725124503274358 1106 32-33 0.9548494878617362 1106 34-35 0.8402864592320398 1106 36-37 1.0025069294664988 1106 38-39 1.155026309239581 1106 40-41 1.2143735123877164 1106 42-43 0.7697336334260214 1106 44-45 0.9411215930693331 1106 46-47 0.9894738217371923 1106 48-49 0.7534769602254094 1106 50-51 0.7514310631450165 1106 52-53 0.5394519233908 1106 54-55 0.5148103163409559 1106 56-57 0.7822639654019561 1106 58-59 0.5990571709725536 1106 60-61 0.5824477948173232 1106 62-63 0.5457852100428937 1106 64-65 0.6808706974215717 1106 66-67 0.5950765237480091 1106 68-69 0.7421732776276109 1106 70-71 0.6475355781656624 1106 72-73 0.685186483632485 1106 74-75 0.5607930274063264 1106 76-77 0.7333008682310123 1106 78-79 0.6101134945221531 1106 80-81 0.6965051566513267 1106 82-83 0.6306455418373211 1106 84-85 0.5988215822401912 1106 86-87 0.6588673093392039 1106 88-89 0.5106427208854072 1106 90-91 0.814164117372858 1106 92-93 1.1013763488890795 1106 94-95 0.9498926884023504 1106 96-97 0.6688749877525559 1106 98-99 0.45329196549294437 1106 100-101 0.4161968344834399 1107 1 14.615277112600182 1107 2 7.952772218859305 1107 3 3.6701709536302474 1107 4 2.0947133498597665 1107 5 1.2843007813936964 1107 6 1.0681942238086535 1107 7 0.8619782221226941 1107 8 0.7983721020624515 1107 9 0.6338283673798557 1107 10-11 0.5766686485471482 1107 12-13 0.5163392108414939 1107 14-15 0.6546570556061297 1107 16-17 0.8976569139390378 1107 18-19 0.672637267233533 1107 20-21 0.5610503414915229 1107 22-23 0.6955151647277731 1107 24-25 0.7545086097770195 1107 26-27 0.605453263746881 1107 28-29 0.6977888965577321 1107 30-31 0.6755672698851214 1107 32-33 0.6552681860064027 1107 34-35 0.35624145827824094 1107 36-37 0.7201771648454312 1107 38-39 0.5701954576539165 1107 40-41 1.037265172675042 1107 42-43 0.8899667826876296 1107 44-45 0.9069029564883593 1107 46-47 0.676887421986919 1107 48-49 0.6332786495727838 1107 50-51 0.7379974503595896 1107 52-53 0.6973127832510251 1107 54-55 0.5746479498425039 1107 56-57 0.4990521646205792 1107 58-59 0.6881287328818466 1107 60-61 0.6920220329511402 1107 62-63 0.4560188888513572 1107 64-65 0.23949563765400228 1107 66-67 0.3184959812195096 1107 68-69 0.3540474058881955 1107 70-71 0.38910912510147355 1107 72-73 0.5097055714740897 1107 74-75 0.4448542399261761 1107 76-77 0.4126074467513554 1107 78-79 0.617877704339147 1107 80-81 0.5196105711595465 1107 82-83 0.6614848333469787 1107 84-85 0.5485973145013041 1107 86-87 0.5629086963679697 1107 88-89 0.524567945153148 1107 90-91 0.8747832195834562 1107 92-93 1.1937058626721608 1107 94-95 1.2320494212654722 1107 96-97 0.6113047283427235 1107 98-99 0.20416416942360627 1107 100-101 0.163695499777619 1108 1 14.292465953382955 1108 2 7.46557810692774 1108 3 3.364589542914647 1108 4 -0.1946875863323001 1108 5 0.2486583545027301 1108 6 0.49621541052295726 1108 7 0.5126928307136183 1108 8 0.4448691909165987 1108 9 0.3829689899160442 1108 10-11 0.35589108652104784 1108 12-13 0.13286553880305974 1108 14-15 0.11128331013203052 1108 16-17 0.4461818182399142 1108 18-19 0.3406582887551366 1108 20-21 0.3718724905283679 1108 22-23 -0.39389943740561506 1108 24-25 0.2502639834203748 1108 26-27 0.18971032460837733 1108 28-29 0.20110626626949824 1108 30-31 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-1.5310964445421078 2312 5 -1.8802134525681282 2312 6 -0.7533250835672973 2312 7 -0.27816138999842366 2312 8 0.042045435960140765 2312 9 0.041337712265807625 2312 10-11 0.1585999122156565 2312 12-13 -0.5256499945939908 2312 14-15 0.2595530370747454 2312 16-17 -0.2530928535466117 2312 18-19 -0.049056140532748316 2312 20-21 -0.025283274162944736 2312 22-23 0.26941778847085374 2312 24-25 0.08504574450545022 2312 26-27 0.2555450854668351 2312 28-29 -0.44870223188022607 2312 30-31 0.08941068985318168 2312 32-33 0.17152036811047822 2312 34-35 -0.5185518170055659 2312 36-37 -0.0035079468486642895 2312 38-39 0.2841770745925061 2312 40-41 0.4608880914784379 2312 42-43 0.448352166762767 2312 44-45 0.36889779700244674 2312 46-47 0.42802078319786574 2312 48-49 0.45349057333671894 2312 50-51 0.27373290965181596 2312 52-53 0.38372562181481484 2312 54-55 0.17296716782858823 2312 56-57 0.06652670077236067 2312 58-59 0.10123955009160923 2312 60-61 0.17807641869141122 2312 62-63 0.14608793487111882 2312 64-65 0.1276684669611754 2312 66-67 0.006089080899855048 2312 68-69 0.22336519695439705 2312 70-71 0.22449740456336897 2312 72-73 0.01670035739569542 2312 74-75 0.07660114407156371 2312 76-77 0.32623299308385256 2312 78-79 0.3960689433658793 2312 80-81 0.2918611554763615 2312 82-83 0.2117888283965037 2312 84-85 0.3945497356637304 2312 86-87 0.4021280210291813 2312 88-89 0.8074024520830605 2312 90-91 0.9904973750455426 2312 92-93 1.3759865465897683 2312 94-95 1.5640178007322874 2312 96-97 1.336997638112127 2312 98-99 1.1402532462051695 2312 100-101 1.1003865118556053 2313 1 -14.231086170169167 2313 2 -7.413099642172545 2313 3 -3.581606413140463 2313 4 -1.0323244958001965 2313 5 -0.5776130647264353 2313 6 -1.0401704723417993 2313 7 -0.40850769189535896 2313 8 -0.2310012779116164 2313 9 -2.4750976357562138 2313 10-11 -0.4643712358539531 2313 12-13 -0.8941732009117374 2313 14-15 -1.0474760373597078 2313 16-17 -1.0108595289422837 2313 18-19 -0.2750535149848332 2313 20-21 -1.1851849615995036 2313 22-23 -2.2026733560386944 2313 24-25 -1.3740905289623058 2313 26-27 -0.4010053485016627 2313 28-29 -0.0027544587179804125 2313 30-31 -1.5057525853381293 2313 32-33 -0.5618453138901458 2313 34-35 -1.5713948198519034 2313 36-37 -1.4770154152830344 2313 38-39 -0.8402343593777033 2313 40-41 -0.3492534724512595 2313 42-43 -0.07830125235444285 2313 44-45 -0.1770227622016094 2313 46-47 -0.9029582860163003 2313 48-49 -0.2859588831543185 2313 50-51 -1.2925621752334813 2313 52-53 -0.24633570372745694 2313 54-55 -0.9457301907058984 2313 56-57 -0.5124049031909514 2313 58-59 -0.2924488654912061 2313 60-61 -1.6284657141640544 2313 62-63 -0.3882932214311374 2313 64-65 -0.09612611728998743 2313 66-67 -1.2904249650968715 2313 68-69 -0.3157715557528782 2313 70-71 0.16777170585783452 2313 72-73 -0.6603521141965274 2313 74-75 -0.2949506848262402 2313 76-77 0.2644342017943728 2313 78-79 0.47736764822109023 2313 80-81 0.25850779677756464 2313 82-83 0.39014979502260516 2313 84-85 0.44898449832891885 2313 86-87 0.09358420741227391 2313 88-89 0.503971097153137 2313 90-91 0.7479491216168412 2313 92-93 1.2759204799994386 2313 94-95 1.1968227724999458 2313 96-97 0.9960880415119853 2313 98-99 1.13535644530009 2313 100-101 1.1331202823238353 2314 1 -14.231086170169167 2314 2 -7.304474042607859 2314 3 -3.676733029983044 2314 4 -1.5300807303467607 2314 5 -1.3922681459726967 2314 6 -6.1984610671291485 2314 7 -1.9484131383225112 2314 8 -4.3581616058209995 2314 9 -1.7274741196176464 2314 10-11 -0.4691254516093508 2314 12-13 -3.0357186146483173 2314 14-15 -2.9794426962360276 2314 16-17 -2.7459369023618976 2314 18-19 -1.3110495980018584 2314 20-21 -2.241342353479496 2314 22-23 -1.2957432809373515 2314 24-25 -2.9780134105354463 2314 26-27 -4.699304634850382 2314 28-29 -2.2511901812979502 2314 30-31 -2.1908456642619996 2314 32-33 -4.2409812050596045 2314 34-35 -2.6120394299737626 2314 36-37 -1.0798413419373531 2314 38-39 -1.3801620076469234 2314 40-41 -2.3652541692475353 2314 42-43 -1.4300906222963796 2314 44-45 -2.734565338831789 2314 46-47 -2.3746051542888296 2314 48-49 -1.1683749060004125 2314 50-51 -2.426093972015419 2314 52-53 -1.0857715220775042 2314 54-55 -0.3782128125096058 2314 56-57 -1.1356358991695252 2314 58-59 -1.3260730298184669 2314 60-61 -0.6060673398118297 2314 62-63 -2.7349766274984937 2314 64-65 -0.9480251170378864 2314 66-67 -2.031117236924665 2314 68-69 -2.111360969336541 2314 70-71 -2.7214751275223357 2314 72-73 -2.673250530522054 2314 74-75 -2.1163076351391723 2314 76-77 -0.8727725910234199 2314 78-79 -0.37204678147861614 2314 80-81 -0.18830131900977065 2314 82-83 -0.6388101432641555 2314 84-85 -0.31992854809093174 2314 86-87 -0.23067757148601586 2314 88-89 0.17151795519180624 2314 90-91 0.4908653874351643 2314 92-93 1.1142060721468674 2314 94-95 1.0758042097893963 2314 96-97 0.9296624947325078 2314 98-99 0.8734799377260458 2314 100-101 0.9281957692437341 2315 1 -14.231086170169167 2315 2 -7.416448417945608 2315 3 -4.516640789399499 2315 4 -2.6950653174903785 2315 5 -3.7547894884682336 2315 6 -2.63861954830044 2315 7 -3.029428935974625 2315 8 -3.6405309697725805 2315 9 -2.3000395243785974 2315 10-11 -4.382941852528653 2315 12-13 -4.190779204205846 2315 14-15 -3.0931674453302804 2315 16-17 -4.3648285986592334 2315 18-19 -1.424718493890424 2315 20-21 -3.1814526037649387 2315 22-23 -4.255371138255931 2315 24-25 -4.908134676387245 2315 26-27 -4.680693295361717 2315 28-29 -2.544859765823702 2315 30-31 -3.1940441473677907 2315 32-33 -4.45457372004666 2315 34-35 -3.8320113666948394 2315 36-37 -5.122457403584569 2315 38-39 -3.3924970238482963 2315 40-41 -4.639733709364066 2315 42-43 -3.2490214289796313 2315 44-45 -1.965158321145907 2315 46-47 -1.4548650280842423 2315 48-49 -1.0115687394910964 2315 50-51 -0.8166513896365437 2315 52-53 -0.6045746680454513 2315 54-55 -2.0633854422835434 2315 56-57 -4.185829072409614 2315 58-59 -3.5699765538534827 2315 60-61 -4.168430072345149 2315 62-63 -2.338898941866095 2315 64-65 -2.9807155383576713 2315 66-67 -1.7354513931180868 2315 68-69 -1.1595861384469082 2315 70-71 -1.8604894853445195 2315 72-73 -1.8663303971892802 2315 74-75 -2.445355494371224 2315 76-77 -2.4661958826249943 2315 78-79 -1.5082996253924286 2315 80-81 -1.2251587180219197 2315 82-83 -0.8737069764793866 2315 84-85 -0.687580566060678 2315 86-87 -1.3023857281041877 2315 88-89 -1.7359298003766952 2315 90-91 -1.3006098232037004 2315 92-93 -0.4372578600870263 2315 94-95 -0.3157075748427438 2315 96-97 -1.3717335594290674 2315 98-99 -0.8776294383581629 2315 100-101 -0.3338204237176594 2316 1 -14.231086170169167 2316 2 -7.336733014900771 2316 3 -4.878063999809868 2316 4 -2.6033453873960646 2316 5 -1.9545496492020789 2316 6 -1.0352573884445206 2316 7 -4.559398331725312 2316 8 -2.719528188313994 2316 9 -0.9188455996913092 2316 10-11 -4.426908538613567 2316 12-13 -3.646385607304012 2316 14-15 -1.7296204497568155 2316 16-17 -3.0314298398543613 2316 18-19 -2.187274799965742 2316 20-21 -3.192895095672853 2316 22-23 -2.7951128174692457 2316 24-25 -3.3693084837035485 2316 26-27 -4.084065813894519 2316 28-29 -3.3479187548988847 2316 30-31 -2.243913997259277 2316 32-33 -2.4835844253802577 2316 34-35 -3.78202523809135 2316 36-37 -2.0568281344763975 2316 38-39 -2.9954899635382333 2316 40-41 -2.496373595603231 2316 42-43 -6.361793446808861 2316 44-45 -3.2075755901884335 2316 46-47 -4.474741380353102 2316 48-49 -4.16764233430068 2316 50-51 -3.075114223524391 2316 52-53 -4.26224514007292 2316 54-55 -4.356369977086647 2316 56-57 -4.3119553221173135 2316 58-59 -3.0186250798445293 2316 60-61 -2.7095708139443673 2316 62-63 -1.8350444371749468 2316 64-65 -2.2220550178392884 2316 66-67 -2.479805914219021 2316 68-69 -2.496304032702252 2316 70-71 -2.506244658529287 2316 72-73 -3.481769261509072 2316 74-75 -4.080009191495819 2316 76-77 -3.7278228564144626 2316 78-79 -3.951651636821847 2316 80-81 -3.9075118145483785 2316 82-83 -3.1904315987466454 2316 84-85 -3.0839846949677465 2316 86-87 -3.0637279664809327 2316 88-89 -2.9024382237245376 2316 90-91 -2.321729088496596 2316 92-93 -2.1528216695366034 2316 94-95 -1.4140657758379973 2316 96-97 -1.4659845706820782 2316 98-99 -1.4436707469154566 2316 100-101 -1.325147339457514 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14310.0 3 4210.0 4 4759.0 5 5580.0 6 6714.0 7 8567.0 8 10829.0 9 13271.0 10 16249.0 11 19841.0 12 23884.0 13 26505.0 14 29549.0 15 33049.0 16 36678.0 17 38883.0 18 34566.0 19 34040.0 20 36204.0 21 39341.0 22 44172.0 23 48806.0 24 54567.0 25 61205.0 26 68416.0 27 77997.0 28 88059.0 29 99621.0 30 113643.0 31 129196.0 32 148560.0 33 171790.0 34 198720.0 35 230145.0 36 242423.0 37 147222.0 38 19390.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.934379780203294 18.788716018977492 13.613483198241196 22.663421002578016 2 15.396921876668257 21.615001710815804 39.801898459088804 23.18617795342714 3 19.35739141988795 26.20130651018146 30.505784940485505 23.935517129445085 4 12.365381006361396 16.78814671187559 38.51443411843021 32.332038163332804 5 12.90388636624525 38.33440485536135 34.47499134863346 14.286717429759943 6 30.167521637843546 36.69338337687048 18.368624990168918 14.770469995117056 7 26.64853826627718 34.05510444860851 22.05317804318429 17.243179241930022 8 25.268007207425462 33.768108992962496 21.079111757154852 19.88477204245719 9 26.658732194750634 16.60237781824452 21.265660418620495 35.473229568384355 10-11 24.355123500320214 26.398022615619 28.27618875812574 20.970665125935046 12-13 26.390882154227956 24.472889523649712 28.084822999089283 21.05140532303305 14-15 23.000105778069198 25.43607166748086 27.698565729293524 23.865256825156422 16-17 21.34026454454574 28.652606858509337 28.0865247648299 21.920603832115024 18-19 21.046879403093048 27.632034959770184 30.548980269937204 20.772105367199558 20-21 22.577139029896394 26.386466308391203 30.013037760281907 21.023356901430493 22-23 21.843904932922232 26.727280186574347 29.858859493821356 21.569955386682064 24-25 21.92465933603409 26.5125480086284 30.302078181722525 21.260714473614982 26-27 21.832370037074245 26.378857621684453 30.62272021171334 21.16605212952796 28-29 21.19586317684904 27.18096591668317 30.473581531218823 21.14958937524897 30-31 21.547719216867495 26.881570844633316 30.222528465855497 21.34818147264369 32-33 21.279767604243695 27.071016314757863 30.19623333348766 21.45298274751078 34-35 21.967973498094924 26.823941771220756 30.034987429943076 21.173097300741244 36-37 21.910731462242836 26.561528278847526 30.226203009195558 21.301537249714087 38-39 21.640063273390233 26.965475441379024 30.282636991539214 21.11182429369153 40-41 21.713285036147152 26.895884753230774 30.216302277889184 21.174527932732886 42-43 21.32596887312273 27.22691932352485 29.59558788726368 21.85152391608874 44-45 21.770853123994687 27.042675030450003 29.3211285048012 21.86534334075411 46-47 22.00305281693179 27.34815518815778 28.823928899207583 21.82486309570284 48-49 22.03124510026141 27.215071190768274 28.87725193300935 21.87643177596096 50-51 21.688202840788065 27.6504732822628 28.95773504780812 21.703588829141022 52-53 21.830561286791024 27.973893637626844 28.44856625542128 21.746978820160848 54-55 21.423632008416952 27.431530690068424 28.66900204255925 22.475835258955378 56-57 21.886064262192406 27.767234914931127 28.154501849204344 22.19219897367212 58-59 22.183803561056465 27.43616935561064 28.219806259712627 22.160220823620268 60-61 21.951954864612564 27.329181700189082 28.857988099698943 21.860875335499408 62-63 21.93599358694497 27.33326951137276 28.727519854065125 22.003217047617145 64-65 21.701097609234452 27.751801841697958 28.51373068696457 22.033369862103015 66-67 21.563534171460407 27.511251554051537 28.285726248338122 22.639488026149934 68-69 21.483952362088854 27.609514875644827 28.48812050049638 22.418412261769944 70-71 21.767017827348454 27.468003139195552 28.217749078965916 22.54722995449008 72-73 21.73135413862963 27.117600773922174 28.42935003458617 22.721695052862025 74-75 21.520247434850585 27.871195955459875 28.05717626112435 22.551380348565196 76-77 21.772178971412647 27.10002427134905 28.261575675771827 22.866221081466477 78-79 21.473771201282517 27.30540792682105 28.429421115736435 22.79139975615999 80-81 21.64112040225657 27.64543456436625 28.068262026721534 22.645183006655646 82-83 21.637373108667415 27.40958028903239 28.19771841348551 22.755328188814683 84-85 21.610893857569963 27.290554212567674 28.018419041398445 23.080132888463922 86-87 21.58707550080414 27.26776632493332 28.16823096275026 22.976927211512276 88-89 21.76866854127789 26.97750192936514 27.97119600434636 23.282633525010613 90-91 21.473558262895544 26.9253252820177 28.298062532932228 23.30305392215453 92-93 21.467928402502455 27.245733582280035 28.332767641025196 22.95357037419231 94-95 21.516744751582188 27.125828143577447 28.082908665909954 23.27451843893041 96-97 21.284495506864097 26.89196112216693 28.189903293927543 23.63364007704143 98-99 21.56908004195221 26.95223092248529 27.65876342070569 23.81992561485681 100-101 21.60443323808206 26.746211903439683 28.026849781604398 23.62250507687386 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5379.0 1 3805.5 2 2053.5 3 1695.0 4 1475.5 5 1540.5 6 2140.5 7 4249.0 8 5534.5 9 4800.5 10 3951.5 11 3494.5 12 3491.0 13 3601.0 14 3712.0 15 3880.0 16 4090.0 17 4267.0 18 4647.5 19 5138.0 20 5666.5 21 6560.0 22 7771.0 23 9119.5 24 10887.0 25 13284.0 26 16094.5 27 19349.0 28 23050.5 29 26864.5 30 30974.0 31 35518.0 32 39896.5 33 44390.5 34 49372.5 35 54239.0 36 58679.0 37 62455.5 38 64647.0 39 65549.5 40 67603.0 41 70410.5 42 72524.5 43 75790.0 44 79211.0 45 81578.5 46 86798.0 47 102071.5 48 113803.0 49 106139.5 50 92872.5 51 85214.5 52 80848.5 53 77468.0 54 72731.5 55 66145.5 56 59592.0 57 53051.0 58 46739.0 59 40167.5 60 33062.0 61 26558.0 62 20644.0 63 15196.5 64 11155.5 65 8230.5 66 6002.5 67 4427.0 68 3110.5 69 2163.5 70 1534.5 71 1102.0 72 752.0 73 505.0 74 380.5 75 291.5 76 198.0 77 122.5 78 69.5 79 43.5 80 29.0 81 19.5 82 16.5 83 12.5 84 11.5 85 7.0 86 4.5 87 5.0 88 6.0 89 3.5 90 3.0 91 3.0 92 2.5 93 2.0 94 2.5 95 3.5 96 3.5 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.96853686070811 2 0.08777559015435064 3 0.11877003299354241 4 0.1910903996183232 5 0.11906401822372444 6 0.14317080709865135 7 0.15064643152328028 8 0.14800056445164195 9 0.1369971172648286 10-11 0.12519571016752118 12-13 0.13748009300012767 14-15 0.1449977153147826 16-17 0.13964298433646694 18-19 0.14623665307054975 20-21 0.17345128580740105 22-23 0.19064942177305014 24-25 0.21826303446514844 26-27 0.2112073889407796 28-29 0.20988445540496048 30-31 0.22389075172863315 32-33 0.2152391863832761 34-35 0.2011698932245644 36-37 0.18857052621676287 38-39 0.18485371294946143 40-41 0.1847067203343704 42-43 0.17855402944556065 44-45 0.1946812192155466 46-47 0.21979595745109767 48-49 0.22290380131302204 50-51 0.23241632340391222 52-53 0.25921097724050346 54-55 0.24633862394753286 56-57 0.2234497738833601 58-59 0.1819978564276931 60-61 0.19224534159403833 62-63 0.1961091474764308 64-65 0.18506370239959144 66-67 0.19413524664520854 68-69 0.18275381844816116 70-71 0.1960041527513658 72-73 0.21513419165821107 74-75 0.24444871889636263 76-77 0.24541467036696077 78-79 0.24123588030937324 80-81 0.24659061128768892 82-83 0.23785505016227984 84-85 0.20763756828856916 86-87 0.22019493740634472 88-89 0.22218983718257998 90-91 0.2091494923295054 92-93 0.20694460310314008 94-95 0.24694759335290994 96-97 0.27321727356417613 98-99 0.2915493525605274 100-101 0.28665659837249774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2381072.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.17241261809683 #Duplication Level Percentage of deduplicated Percentage of total 1 81.17840948467773 56.15306436554953 2 11.310935503512873 15.648093954913467 3 3.663100636344336 7.601565260364704 4 1.545830317220627 4.277152501613949 5 0.8061009054577808 2.7879972222073546 6 0.4394778298871246 1.8239845071274772 7 0.26927011123913347 1.303824426824792 8 0.17314647678789477 0.9581567628593586 9 0.12867719476029008 0.8010820809448195 >10 0.4621978879896562 5.149807765708136 >50 0.013998279579983064 0.652341366555004 >100 0.0068816014936875815 0.9206744282315585 >500 0.0011570382010325326 0.5624115232896328 >1k 7.48671777138583E-4 1.085229200012374 >5k 6.80610706489621E-5 0.27461463379776047 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5833 0.2449736925216877 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4215 0.17702110645961147 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3809 0.1599699631090534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2882 0.12103791905494668 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0998945013002548E-5 0.0 12-13 0.0 2.0998945013002548E-5 0.0 6.299683503900764E-5 0.0 14-15 0.0 4.1997890026005096E-5 0.0 8.399578005201019E-5 0.0 16-17 0.0 4.1997890026005096E-5 0.0 1.4699261509101784E-4 0.0 18-19 0.0 4.1997890026005096E-5 0.0 2.30988395143028E-4 4.1997890026005096E-5 20-21 0.0 4.1997890026005096E-5 0.0 3.779810102340458E-4 4.1997890026005096E-5 22-23 0.0 4.1997890026005096E-5 0.0 0.0010919451406761324 4.1997890026005096E-5 24-25 0.0 4.1997890026005096E-5 0.0 0.0018269082161312216 4.1997890026005096E-5 26-27 0.0 4.1997890026005096E-5 0.0 0.002750861796703334 4.1997890026005096E-5 28-29 0.0 4.1997890026005096E-5 0.0 0.008063594884992979 4.1997890026005096E-5 30-31 0.0 8.399578005201019E-5 0.0 0.02127193129817158 4.1997890026005096E-5 32-33 2.0998945013002548E-5 8.399578005201019E-5 0.0 0.049011537660347944 4.1997890026005096E-5 34-35 4.1997890026005096E-5 8.399578005201019E-5 0.0 0.090736441401184 4.1997890026005096E-5 36-37 6.299683503900764E-5 8.399578005201019E-5 0.0 0.1517383766639564 4.1997890026005096E-5 38-39 8.399578005201019E-5 8.399578005201019E-5 0.0 0.23571315777095359 4.1997890026005096E-5 40-41 8.399578005201019E-5 8.399578005201019E-5 0.0 0.3346811856172346 4.1997890026005096E-5 42-43 8.399578005201019E-5 8.399578005201019E-5 0.0 0.43322923456325557 4.1997890026005096E-5 44-45 8.399578005201019E-5 8.399578005201019E-5 0.0 0.5505293414058878 4.1997890026005096E-5 46-47 8.399578005201019E-5 8.399578005201019E-5 0.0 0.6894793605569256 4.1997890026005096E-5 48-49 8.399578005201019E-5 8.399578005201019E-5 0.0 0.8377949091837626 4.1997890026005096E-5 50-51 8.399578005201019E-5 8.399578005201019E-5 0.0 0.9951400041661906 4.1997890026005096E-5 52-53 8.399578005201019E-5 8.399578005201019E-5 0.0 1.1482223133109792 4.1997890026005096E-5 54-55 8.399578005201019E-5 8.399578005201019E-5 0.0 1.321127626548042 4.1997890026005096E-5 56-57 8.399578005201019E-5 8.399578005201019E-5 0.0 1.5241244279887378 4.1997890026005096E-5 58-59 8.399578005201019E-5 8.399578005201019E-5 0.0 1.7421984719487693 4.1997890026005096E-5 60-61 8.399578005201019E-5 8.399578005201019E-5 0.0 1.9838753301034155 4.1997890026005096E-5 62-63 8.399578005201019E-5 8.399578005201019E-5 0.0 2.2325658358924048 4.1997890026005096E-5 64-65 8.399578005201019E-5 8.399578005201019E-5 0.0 2.481067351176277 4.1997890026005096E-5 66-67 8.399578005201019E-5 8.399578005201019E-5 0.0 2.769886840885114 4.1997890026005096E-5 68-69 8.399578005201019E-5 8.399578005201019E-5 0.0 3.0965674284523947 4.1997890026005096E-5 70-71 8.399578005201019E-5 8.399578005201019E-5 0.0 3.4405721456554024 4.1997890026005096E-5 72-73 8.399578005201019E-5 8.399578005201019E-5 0.0 3.7969662404160816 4.1997890026005096E-5 74-75 8.399578005201019E-5 8.399578005201019E-5 0.0 4.171776409953164 4.1997890026005096E-5 76-77 8.399578005201019E-5 8.399578005201019E-5 0.0 4.567459530833171 4.1997890026005096E-5 78-79 8.399578005201019E-5 8.399578005201019E-5 0.0 5.01994479797335 4.1997890026005096E-5 80-81 8.399578005201019E-5 8.399578005201019E-5 0.0 5.505692394014124 4.1997890026005096E-5 82-83 8.399578005201019E-5 8.399578005201019E-5 0.0 5.992867918315785 4.1997890026005096E-5 84-85 8.399578005201019E-5 8.399578005201019E-5 0.0 6.512822795782739 4.1997890026005096E-5 86-87 8.399578005201019E-5 8.399578005201019E-5 0.0 7.061126249017249 4.1997890026005096E-5 88-89 8.399578005201019E-5 8.399578005201019E-5 0.0 7.631352600845334 4.1997890026005096E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 8705 0.0 32.421394 1 TACATGG 10600 0.0 26.56276 2 GTATAGC 965 0.0 26.019972 1 ACATGGG 10490 0.0 25.716492 3 GAGTACT 9565 0.0 24.131363 12-13 GTGTAGC 1180 0.0 22.981369 1 GTACCGT 125 2.3562179E-4 22.655405 6 CATGGGA 7110 0.0 22.190338 4 GTACTTT 10840 0.0 21.448597 14-15 AGTACTT 10320 0.0 21.199615 12-13 CATGGGG 3955 0.0 21.14043 4 TAGCCCT 1390 0.0 21.069973 4 GTATCAA 26215 0.0 19.271357 1 TGTACCG 230 2.1893484E-6 18.46869 5 ATGGGAG 2895 0.0 17.770489 5 GTAGCCC 910 0.0 17.651785 3 GTCCTAC 570 4.7926915E-7 17.620543 1 ACTTTTT 13805 0.0 17.613764 16-17 CCTATAC 570 0.0 17.40586 3 AGTACAT 7325 0.0 17.284626 2 >>END_MODULE