FastQCFastQC Report
Fri 27 May 2016
SRR939662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381072
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140070.5882644455942533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110570.4643706700175383No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90850.3815508308862563No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77750.3265335949521896No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51870.21784305556488845No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA44890.18852852832673686No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT39820.1672355980835523No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA38500.1616918766001196No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35280.14816855601174597No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34900.14657263619075778No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.12817756035936753No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT26020.10927850984766525No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24870.10444875249467467No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24310.10209687065321837No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108600.025.1620271
TACATGG110300.024.5953082
ACATGGG107900.023.9572473
TACCGCG1204.671165E-922.22907662-63
GAGTACT100700.021.8841112-13
CATGGGG39150.021.3264874
AGTACTT104450.021.03072412-13
CATGGGA73200.019.9124744
TACGCGG1001.0481212E-519.4560464-65
GTACTTT114050.019.23982414-15
GTAGGAC6700.019.0093293
ATGGGAG26500.018.5125085
GTATCAA266400.017.7690261
GCTTACG2750.017.38111560-61
TACTTTT128550.017.12464314-15
ACTTTTT133950.016.64508616-17
GTCCTAC6600.016.4467751
GGTATCA212950.016.2008061
AGAGTAC211700.016.00975810-11
AACGCAG295400.015.8295586
Produced by FastQC (version 0.11.5)