##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939662_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2381072 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.478928818616154 31.0 31.0 34.0 30.0 34.0 2 31.67436851972557 31.0 31.0 34.0 30.0 34.0 3 31.721358698938964 31.0 31.0 34.0 30.0 34.0 4 35.52172466855265 37.0 35.0 37.0 33.0 37.0 5 35.28330012700162 37.0 35.0 37.0 33.0 37.0 6 35.29393945248191 37.0 35.0 37.0 32.0 37.0 7 35.20417568221372 37.0 35.0 37.0 32.0 37.0 8 35.170777280149444 37.0 35.0 37.0 32.0 37.0 9 36.75509476403906 39.0 35.0 39.0 32.0 39.0 10-11 36.63747253337992 39.0 35.0 39.0 32.0 39.0 12-13 36.576855718768684 39.0 35.0 39.0 32.0 39.0 14-15 37.607521318128974 39.0 36.0 41.0 32.0 41.0 16-17 37.577555613605966 39.0 36.0 41.0 32.0 41.0 18-19 37.584140462783154 39.0 36.0 41.0 32.0 41.0 20-21 37.532888757668815 39.0 36.0 41.0 32.0 41.0 22-23 37.39592397877931 39.0 36.0 41.0 32.0 41.0 24-25 37.25781643730219 39.0 36.0 41.0 31.0 41.0 26-27 36.96319661900186 39.0 36.0 40.0 31.0 41.0 28-29 36.76342273564176 39.0 35.5 40.0 30.0 41.0 30-31 36.506348191066884 38.0 35.0 40.0 30.0 41.0 32-33 36.29741225800816 38.0 35.0 40.0 30.0 41.0 34-35 36.23023936277441 38.0 35.0 40.0 30.0 41.0 36-37 36.10766957068077 38.0 35.0 40.0 30.0 41.0 38-39 36.11162682186847 38.0 35.0 40.0 30.0 41.0 40-41 35.99101350148169 38.0 35.0 40.0 29.5 41.0 42-43 35.788126524523406 38.0 35.0 40.0 29.0 41.0 44-45 35.53188080830819 38.0 34.5 40.0 27.5 41.0 46-47 35.17157901987004 38.0 34.0 40.0 26.5 41.0 48-49 34.8290440188285 38.0 34.0 40.0 25.5 41.0 50-51 34.56749396910299 38.0 33.0 40.0 24.0 41.0 52-53 34.33596779097818 38.0 33.0 40.0 24.0 41.0 54-55 34.057463403038625 38.0 33.0 40.0 23.5 41.0 56-57 33.291919983940005 37.0 32.0 40.0 19.0 41.0 58-59 30.344015426664964 35.5 26.5 39.0 7.5 40.5 60-61 30.054498352002796 34.5 26.0 39.0 9.5 40.0 62-63 31.77210537942574 35.0 30.5 39.0 13.0 40.0 64-65 31.80169625278026 35.5 31.0 39.0 9.5 40.0 66-67 27.06277697608472 32.0 18.5 37.0 2.0 39.5 68-69 19.735536346653944 20.0 8.0 32.5 2.0 37.0 70-71 17.79453876237258 15.5 7.5 29.0 2.0 35.0 72-73 19.523931027705167 20.0 7.5 31.0 2.0 35.5 74-75 20.93432096971448 23.5 9.0 32.5 2.0 36.0 76-77 22.659522265601375 27.5 11.0 33.0 2.0 35.5 78-79 24.782451769623094 30.5 13.0 34.5 2.0 36.5 80-81 26.342470954259255 32.0 22.5 35.0 2.0 36.5 82-83 27.430210636217637 33.0 26.0 35.0 2.0 36.0 84-85 27.4592265584577 33.5 26.0 35.0 2.0 36.0 86-87 27.1905666859297 33.0 26.0 35.0 2.0 36.0 88-89 26.75904697547995 33.0 25.5 35.0 2.0 35.0 90-91 24.99534957363742 32.0 15.5 34.5 2.0 35.0 92-93 23.472604146367686 31.0 2.0 34.0 2.0 35.0 94-95 19.83871067317578 27.5 2.0 34.0 2.0 35.0 96-97 17.172586549251765 18.5 2.0 33.0 2.0 35.0 98-99 17.457642188056475 21.5 2.0 33.0 2.0 35.0 100-101 16.71056482122338 10.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2685369635988657 1101 2 0.2355281357374821 1101 3 0.19702830305432784 1101 4 0.20089343572524854 1101 5 0.38802320507812027 1101 6 0.31948717818018935 1101 7 0.33712009688309763 1101 8 0.32969278852063155 1101 9 0.38471552860523417 1101 10-11 0.37701254300844056 1101 12-13 0.3154469507961153 1101 14-15 0.41037245285004076 1101 16-17 0.3648256345397911 1101 18-19 0.5517455515437817 1101 20-21 0.4498556515588845 1101 22-23 0.4330890463299326 1101 24-25 0.3526276622508462 1101 26-27 0.40463069736539126 1101 28-29 0.4460384041911496 1101 30-31 0.4052892371054426 1101 32-33 0.3455774485462868 1101 34-35 0.3028407569371012 1101 36-37 0.21652218217753472 1101 38-39 0.18740487007661244 1101 40-41 0.2598972160418782 1101 42-43 0.39278125572324285 1101 44-45 0.3319290820982914 1101 46-47 0.17417482311590504 1101 48-49 0.11387514653405617 1101 50-51 0.19154417709894744 1101 52-53 0.10245342431155535 1101 54-55 0.20328277015409668 1101 56-57 -3.202538728507264 1101 58-59 -9.894765881948551 1101 60-61 -11.48782419924034 1101 62-63 -3.824456871646067 1101 64-65 -1.0628006064529814 1101 66-67 -2.070881065189269 1101 68-69 -4.650917696982766 1101 70-71 -4.322099703045822 1101 72-73 -3.287969297180389 1101 74-75 -6.018585422186913 1101 76-77 -4.713610777564362 1101 78-79 1.2248663753274798 1101 80-81 1.772815391198371 1101 82-83 0.4642242508546275 1101 84-85 0.23489619767800107 1101 86-87 0.1532406601054639 1101 88-89 0.15434594741217822 1101 90-91 1.4389340325641058 1101 92-93 0.8664337331956524 1101 94-95 -8.178227174611063 1101 96-97 -9.164788739249307 1101 98-99 -8.177927817985445 1101 100-101 -9.370054163924834 1102 1 0.3091434333392371 1102 2 0.2688378111557519 1102 3 0.2581054984054596 1102 4 0.24362200220156183 1102 5 0.2878389287731338 1102 6 0.2664714325909614 1102 7 0.2711823859232183 1102 8 0.26752982258438607 1102 9 0.24775663499286793 1102 10-11 0.37810250998466444 1102 12-13 0.36475810407386433 1102 14-15 0.4799532698691422 1102 16-17 0.3891896076099073 1102 18-19 0.3390796572563488 1102 20-21 0.341946640265121 1102 22-23 0.31278289223114797 1102 24-25 0.3240526107082147 1102 26-27 0.10852266766224261 1102 28-29 0.19498273815089817 1102 30-31 0.3022841649457533 1102 32-33 0.15966883864645354 1102 34-35 0.17943620658953563 1102 36-37 0.21146872380391102 1102 38-39 0.2301081669804006 1102 40-41 0.14191358196718795 1102 42-43 0.28980756527094087 1102 44-45 0.11323151418061883 1102 46-47 0.24071188819645783 1102 48-49 0.2569559361853919 1102 50-51 0.19871781646474318 1102 52-53 0.18178692793322426 1102 54-55 0.03519112462142715 1102 56-57 -3.2750381355389564 1102 58-59 -9.554783214868326 1102 60-61 -7.780330265762263 1102 62-63 -2.135426511668687 1102 64-65 -0.6382248068580232 1102 66-67 -1.9794770986874148 1102 68-69 -4.916497437536325 1102 70-71 -4.855557073185764 1102 72-73 -3.6241525882457246 1102 74-75 -4.260853206674863 1102 76-77 -4.228968383067105 1102 78-79 1.611803192273598 1102 80-81 2.0454667805687308 1102 82-83 0.6581893843181135 1102 84-85 0.2355367859025641 1102 86-87 0.229140785997199 1102 88-89 0.008640588803189075 1102 90-91 1.311209480027113 1102 92-93 1.0700792111281068 1102 94-95 -8.439275907483403 1102 96-97 -9.475161032120916 1102 98-99 -8.411041996313822 1102 100-101 -9.25334650642333 1103 1 0.20227194643639734 1103 2 0.1687317970647122 1103 3 0.20945893806577232 1103 4 0.17544721146895625 1103 5 0.14631839958612858 1103 6 0.17358900884380546 1103 7 0.2886196678213153 1103 8 0.23997668440163977 1103 9 0.397277742564043 1103 10-11 0.2960085384317708 1103 12-13 0.1756672025123649 1103 14-15 0.3228549328271626 1103 16-17 0.2940565412903595 1103 18-19 0.3084045904453774 1103 20-21 0.32571306002570566 1103 22-23 0.21261493305761547 1103 24-25 0.26153820286869234 1103 26-27 0.32873967905875645 1103 28-29 0.2799568109417194 1103 30-31 0.21398274397042627 1103 32-33 0.11434246570875217 1103 34-35 0.17198191826432918 1103 36-37 0.3331840814910336 1103 38-39 0.33156247877226264 1103 40-41 0.41733142656819666 1103 42-43 0.43996839817175015 1103 44-45 0.35872971138891074 1103 46-47 0.24958815263535428 1103 48-49 0.2367470000809675 1103 50-51 0.2329078784719485 1103 52-53 0.2839780238538978 1103 54-55 0.11760914315181026 1103 56-57 0.6011301073050959 1103 58-59 -7.735424134236883 1103 60-61 -7.3193255579359935 1103 62-63 -1.9909907675270695 1103 64-65 -0.2754132351715164 1103 66-67 -1.7478504587819437 1103 68-69 -3.139603695266519 1103 70-71 -3.6584494599108126 1103 72-73 -2.93937411697444 1103 74-75 -3.868160293468378 1103 76-77 -0.5056398822554371 1103 78-79 3.3625501567920075 1103 80-81 2.8090621732349845 1103 82-83 1.2047516392985642 1103 84-85 0.8202516038564909 1103 86-87 0.8179117127370468 1103 88-89 0.6477695029041648 1103 90-91 1.8797803569348162 1103 92-93 1.870740798413042 1103 94-95 -6.952563559050422 1103 96-97 -8.545666528150909 1103 98-99 -7.942301745330983 1103 100-101 -9.238841349279527 1104 1 0.12616019900183773 1104 2 0.19573028233341816 1104 3 0.21857216022991466 1104 4 0.1165380313439357 1104 5 0.25216024883835786 1104 6 0.15559757454261103 1104 7 0.2861160610007545 1104 8 0.17817254742512745 1104 9 0.15774702258665485 1104 10-11 0.21492974573661883 1104 12-13 0.2204284456587331 1104 14-15 0.3542506890460899 1104 16-17 0.24387931706213095 1104 18-19 0.2547249667814384 1104 20-21 0.20327266923569454 1104 22-23 0.2771235187572785 1104 24-25 0.30141548763284476 1104 26-27 0.2882269505156714 1104 28-29 0.3366749809169747 1104 30-31 0.2737944626037532 1104 32-33 0.4076968154943472 1104 34-35 0.23059458573813885 1104 36-37 0.20073509700102932 1104 38-39 0.11370551687522124 1104 40-41 0.16379060882663765 1104 42-43 0.1317378303341954 1104 44-45 0.12267730648630248 1104 46-47 0.27357684281946604 1104 48-49 0.050211041213387375 1104 50-51 0.23770691656423537 1104 52-53 0.24944255034035479 1104 54-55 0.10947379604366603 1104 56-57 0.6611245837360826 1104 58-59 -7.895701889421101 1104 60-61 -7.304364002909647 1104 62-63 -1.9263111745311043 1104 64-65 -0.24423598827564774 1104 66-67 -1.453025546252345 1104 68-69 -3.131977953234859 1104 70-71 -3.783555881118117 1104 72-73 -2.8237834351053444 1104 74-75 -3.4972392175024716 1104 76-77 -0.03074503674202589 1104 78-79 3.7268822016259193 1104 80-81 3.1615862975976228 1104 82-83 1.62673839406677 1104 84-85 1.1721177834801537 1104 86-87 1.2215949365545917 1104 88-89 1.015666949861906 1104 90-91 2.4250940403186405 1104 92-93 2.2856393340839 1104 94-95 -5.920356851564236 1104 96-97 -7.600682625504396 1104 98-99 -5.419657695851539 1104 100-101 -4.737982645974853 1105 1 0.2708647663370414 1105 2 0.202670386418486 1105 3 0.23838536252296905 1105 4 0.20562793178071814 1105 5 0.2619397862505082 1105 6 0.24400622866308908 1105 7 0.26603933132608404 1105 8 0.2283849030004177 1105 9 0.318700741043763 1105 10-11 0.28003090364097716 1105 12-13 0.19215070428958114 1105 14-15 0.3189235902187093 1105 16-17 0.3497991018338027 1105 18-19 0.48335962449103675 1105 20-21 0.4217484948036585 1105 22-23 0.5027487201545355 1105 24-25 0.34389097282500813 1105 26-27 0.25027208131133705 1105 28-29 0.38058469491885205 1105 30-31 0.3100415985162712 1105 32-33 0.2532862609423674 1105 34-35 0.2906502167312084 1105 36-37 0.26811326011293346 1105 38-39 0.27721188886176407 1105 40-41 0.13681747641616937 1105 42-43 0.13077945149922954 1105 44-45 0.1341990435374285 1105 46-47 0.1413199039522155 1105 48-49 -0.04874398531580937 1105 50-51 0.09741816443400353 1105 52-53 0.18319138730728213 1105 54-55 0.1332220986997541 1105 56-57 0.777709971390145 1105 58-59 -8.17011593678281 1105 60-61 -7.4756295906861965 1105 62-63 -2.0984744539858973 1105 64-65 -0.3241663332722382 1105 66-67 -1.5697886252416247 1105 68-69 -3.319173260006787 1105 70-71 -3.742287377719471 1105 72-73 -2.8410279875260223 1105 74-75 -3.396178198215498 1105 76-77 -0.1586015795596225 1105 78-79 3.74804923873711 1105 80-81 3.2684728716526124 1105 82-83 1.6784477269928857 1105 84-85 1.2771956988631317 1105 86-87 1.1730209692999303 1105 88-89 1.0213447445961705 1105 90-91 2.3754796511299254 1105 92-93 2.196265869609139 1105 94-95 -5.941959088149819 1105 96-97 -7.65047116044717 1105 98-99 -5.515771062758503 1105 100-101 -4.853986310951402 1106 1 0.10967169082124428 1106 2 0.168145139337593 1106 3 0.22036186716399442 1106 4 0.08671523024200667 1106 5 0.13026912033704718 1106 6 0.18563644431145576 1106 7 0.20520165323867445 1106 8 0.261727543436173 1106 9 0.06852679972126907 1106 10-11 0.1026314851297272 1106 12-13 0.18998793667260117 1106 14-15 0.1061097832294351 1106 16-17 0.2804023445924102 1106 18-19 0.21634123141085126 1106 20-21 0.1913029653223859 1106 22-23 0.2632167810294632 1106 24-25 0.20745700315364246 1106 26-27 0.12320519196522639 1106 28-29 0.17956893589981604 1106 30-31 0.24792770290438426 1106 32-33 0.3345303009417435 1106 34-35 0.2974402446136466 1106 36-37 0.32749559674413575 1106 38-39 0.30877187367672576 1106 40-41 0.298941802029141 1106 42-43 0.30740601340809093 1106 44-45 0.34895033659842767 1106 46-47 0.43816194579660106 1106 48-49 0.43502683581679946 1106 50-51 0.36774225242434255 1106 52-53 0.6344850637466166 1106 54-55 0.5862386687971295 1106 56-57 1.0465408200942363 1106 58-59 -7.882545582862424 1106 60-61 -7.142996935324529 1106 62-63 -1.5302575501284785 1106 64-65 0.22743755472120597 1106 66-67 1.283645727942826 1106 68-69 -1.7374197047368511 1106 70-71 -1.9904388057874467 1106 72-73 -0.42671514320642245 1106 74-75 -0.4656070228515503 1106 76-77 2.6397792113583733 1106 78-79 4.836299842511085 1106 80-81 3.843060821827006 1106 82-83 2.1727146274816036 1106 84-85 1.7774491608460998 1106 86-87 1.6845623161564198 1106 88-89 1.525308630879362 1106 90-91 2.8402058409274424 1106 92-93 2.702398839814318 1106 94-95 -5.65443667336487 1106 96-97 -7.3017570305813475 1106 98-99 -5.0274469954986 1106 100-101 -4.331459937948651 1107 1 0.11733881704787308 1107 2 0.13344960090921276 1107 3 0.0701695270747571 1107 4 0.05580811957352694 1107 5 0.06693345823624952 1107 6 0.09383686448056494 1107 7 0.019780202069711095 1107 8 0.07216602681397433 1107 9 0.022524197199757623 1107 10-11 0.023113366575650218 1107 12-13 0.09495610811944033 1107 14-15 0.13666293363065307 1107 16-17 0.11096577625423265 1107 18-19 0.123741137764668 1107 20-21 0.15560802333099133 1107 22-23 0.13900080456983233 1107 24-25 0.06689397716806411 1107 26-27 0.030047216060538062 1107 28-29 0.011305344816520346 1107 30-31 0.013479503115952696 1107 32-33 -0.005143891483818663 1107 34-35 0.06126397240054615 1107 36-37 0.12702773745123608 1107 38-39 0.32404473275904166 1107 40-41 0.16256738329197873 1107 42-43 0.16250805801637824 1107 44-45 -0.07103567531296306 1107 46-47 -2.41999977795615E-4 1107 48-49 0.07119601643749718 1107 50-51 0.11824601871023077 1107 52-53 0.0945057470416728 1107 54-55 0.08082838766039657 1107 56-57 0.7456807090318449 1107 58-59 -8.55506436164686 1107 60-61 -7.48944266946847 1107 62-63 -2.0976477295426683 1107 64-65 -0.3704837106418424 1107 66-67 0.6819577895957067 1107 68-69 -2.2738764509779976 1107 70-71 -2.328201287609435 1107 72-73 -0.7565264480098755 1107 74-75 -0.7759431581380731 1107 76-77 2.0943780780661676 1107 78-79 4.063846978374848 1107 80-81 3.0949832162706628 1107 82-83 1.3781943690627365 1107 84-85 1.0212349594523076 1107 86-87 0.7196311696348303 1107 88-89 0.7501011940595639 1107 90-91 1.963796038550612 1107 92-93 1.4876669701361074 1107 94-95 -6.833264801655183 1107 96-97 -8.245069039589218 1107 98-99 -6.085431834355322 1107 100-101 -5.2024690591609986 1108 1 -0.1559552304585452 1108 2 -0.10934176521937644 1108 3 -0.01691495697588863 1108 4 -0.04244549326585201 1108 5 -0.09488765609361138 1108 6 -0.11424120859167175 1108 7 -0.05850521787264995 1108 8 -0.14528958515739276 1108 9 -0.15185321405046892 1108 10-11 -0.12892668814819075 1108 12-13 -0.0667949494892639 1108 14-15 -0.17403832438682088 1108 16-17 -0.13191013850033784 1108 18-19 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2313 1 -0.3645310094568579 2313 2 -0.2995447990843836 2313 3 -0.3133750759712193 2313 4 -0.18023410992771005 2313 5 -0.25092979945969773 2313 6 -0.14544004401586363 2313 7 -0.24984647400263782 2313 8 -0.33169603494412314 2313 9 -0.377605510225294 2313 10-11 -0.3080280461227858 2313 12-13 -0.38536506735515985 2313 14-15 -0.522264270500095 2313 16-17 -0.5581932704172701 2313 18-19 -0.5312653454995768 2313 20-21 -0.5552482943013359 2313 22-23 -0.408330395628397 2313 24-25 -0.46072215796364446 2313 26-27 -0.5797154800334496 2313 28-29 -0.42370314431953204 2313 30-31 -0.5627421507759252 2313 32-33 -0.5282036213737911 2313 34-35 -0.5221743952917706 2313 36-37 -0.5607467267049699 2313 38-39 -0.6271714955867154 2313 40-41 -0.6897831739906195 2313 42-43 -0.6855479951442831 2313 44-45 -0.7254937043416376 2313 46-47 -0.6784137742430545 2313 48-49 -0.7850925391788834 2313 50-51 -0.9852542275990288 2313 52-53 -0.8194431950927097 2313 54-55 -0.9002266587717429 2313 56-57 -0.19625267326836138 2313 58-59 2.9567694392315964 2313 60-61 2.799290989102648 2313 62-63 0.20655094784614292 2313 64-65 -0.36766305875879013 2313 66-67 -2.0268347135005413 2313 68-69 -4.908990459084922 2313 70-71 -3.8019448048719404 2313 72-73 -3.1979495129230067 2313 74-75 -3.4297143024921546 2313 76-77 0.28188115956566406 2313 78-79 2.6975242175872793 2313 80-81 2.0240872363159 2313 82-83 0.5188508622509929 2313 84-85 0.07041569145224003 2313 86-87 0.1893222780180679 2313 88-89 0.2542722275007314 2313 90-91 1.7927179288066704 2313 92-93 3.2372003731692693 2313 94-95 5.499677892357081 2313 96-97 1.698214934969922 2313 98-99 3.051350292146921 2313 100-101 4.081742749102943 2314 1 -0.40461216572879266 2314 2 -0.4334106087862182 2314 3 -0.5368395065839593 2314 4 -0.2066582431461157 2314 5 -0.30799273747806666 2314 6 -0.34676647927627613 2314 7 -0.49261941313635305 2314 8 -0.4097582371487789 2314 9 -0.5796942963113381 2314 10-11 -0.5345249330698252 2314 12-13 -0.665090082433359 2314 14-15 -0.7842364167784339 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1.9484958758014663 2314 82-83 0.4820742990935116 2314 84-85 0.03649725418866723 2314 86-87 0.0652486630156126 2314 88-89 0.20708615333685998 2314 90-91 1.722778513561149 2314 92-93 3.445930071785174 2314 94-95 6.08555317239259 2314 96-97 3.7169771993528755 2314 98-99 5.796564645677233 2314 100-101 6.177305192046507 2315 1 -0.4681650972204352 2315 2 -0.4334320301333392 2315 3 -0.4577266605043526 2315 4 -0.3054624325833686 2315 5 -0.4618919746152699 2315 6 -0.5257949278766034 2315 7 -0.49913084556475695 2315 8 -0.5524798679257117 2315 9 -0.6184209378436236 2315 10-11 -0.5073170624200642 2315 12-13 -0.5660996061277359 2315 14-15 -0.6542577934429943 2315 16-17 -0.5099381251988362 2315 18-19 -0.5368285248573272 2315 20-21 -0.6634416593232473 2315 22-23 -0.7318324163491354 2315 24-25 -0.4750507745423249 2315 26-27 -0.5334969729035208 2315 28-29 -0.5969695752006601 2315 30-31 -0.4959042160234901 2315 32-33 -0.5562609530621572 2315 34-35 -0.4971526459279332 2315 36-37 -0.5132089060355867 2315 38-39 -0.5814613651583684 2315 40-41 -0.693094113437887 2315 42-43 -0.6896508880314727 2315 44-45 -0.7324105761072914 2315 46-47 -0.5617091929685429 2315 48-49 -0.6389280075247541 2315 50-51 -0.5352436464748109 2315 52-53 -0.4707945651330263 2315 54-55 -0.44133993655613324 2315 56-57 0.318673887727968 2315 58-59 3.423538968516386 2315 60-61 3.2729567758790346 2315 62-63 0.7736478657251347 2315 64-65 0.07230243451208551 2315 66-67 -1.8686245308841656 2315 68-69 -4.8709613637332065 2315 70-71 -3.530097001361984 2315 72-73 -2.802828872450558 2315 74-75 -0.4017953995681651 2315 76-77 2.134002316177785 2315 78-79 3.7911443346137297 2315 80-81 2.736554710122725 2315 82-83 1.0215857110204993 2315 84-85 0.7592751245440468 2315 86-87 0.7549458624176033 2315 88-89 0.8537264450246411 2315 90-91 2.5325162768465894 2315 92-93 4.2302512752916535 2315 94-95 6.816279611013467 2315 96-97 4.564356146400357 2315 98-99 6.562750116168868 2315 100-101 6.940073694833647 2316 1 -0.5114087271469607 2316 2 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3.2049907790050973 2316 62-63 0.6857953886680654 2316 64-65 0.08836966540186353 2316 66-67 -1.900987832245768 2316 68-69 -2.838182176879087 2316 70-71 -3.01690622632783 2316 72-73 -0.39826984953019107 2316 74-75 5.646688592686413 2316 76-77 5.745544497530524 2316 78-79 4.935855050613682 2316 80-81 3.1419408887760554 2316 82-83 1.3222813064290406 2316 84-85 1.042423275161262 2316 86-87 0.9797204329940392 2316 88-89 0.7350478019497757 2316 90-91 2.1353972966737125 2316 92-93 3.798066250861588 2316 94-95 6.275290275322593 2316 96-97 4.008021610366516 2316 98-99 6.078965291527933 2316 100-101 6.243863816758349 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 19.0 4 46.0 5 103.0 6 304.0 7 682.0 8 1442.0 9 2603.0 10 4288.0 11 6740.0 12 9665.0 13 13190.0 14 16251.0 15 20968.0 16 27796.0 17 36255.0 18 37120.0 19 34440.0 20 35398.0 21 35890.0 22 36460.0 23 36864.0 24 39375.0 25 41896.0 26 51682.0 27 71836.0 28 101550.0 29 133782.0 30 162584.0 31 181524.0 32 191479.0 33 192545.0 34 197856.0 35 210400.0 36 212168.0 37 164528.0 38 66950.0 39 4390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.998537266107505 19.75829183599569 14.28450804335607 22.958662854540734 2 15.509569911578025 22.2367975039725 38.86736199581391 23.386270588635565 3 19.129499140301025 26.280245906665336 30.972471993636464 23.617782959397175 4 12.63267133459215 17.506148491099808 37.87147133728001 31.98970883702803 5 12.741431516218709 38.69443434663932 34.51016072670576 14.053973410436205 6 30.29899977825114 37.20488082678726 17.822560594555732 14.673558800405868 7 26.493486967214768 34.15562402144916 21.833359092039217 17.517529919296855 8 25.254349828291556 34.43500844787913 20.73188913728318 19.578752586546138 9 26.163803530510627 16.514914290706034 21.19045539152113 36.13082678726221 10-11 24.225684901590544 26.661940504109072 28.16344906831881 20.948925525981572 12-13 26.08152966394968 24.54743073707977 28.19809732759026 21.172942271380286 14-15 22.61743819246966 25.656083569467558 27.629300551308848 24.09717768675393 16-17 21.08630505215824 28.889598653379373 28.09888321287286 21.92521308158953 18-19 20.897033065728486 27.77562770874575 30.4430525809823 20.884286644543472 20-21 22.268352237983564 26.605096359958875 29.93981282380373 21.186738578253827 22-23 21.605835522823334 26.906830200850706 29.776462030547584 21.710872245778372 24-25 21.71010163922171 26.77064377386796 30.143267582753797 21.375987004156535 26-27 21.70451591281059 26.629131705129083 30.459953071498557 21.206399310561768 28-29 21.054102624515497 27.384400741017927 30.32484841765121 21.23664821681536 30-31 21.4282789299374 27.06171262180206 30.094203460069842 21.415804988190708 32-33 21.178842598387032 27.22385648777759 30.004002597278234 21.593298316557146 34-35 21.81456993972153 26.94259738159811 29.952888085315934 21.28994459336443 36-37 21.864285020708532 26.70448693994168 30.04109760060127 21.390130438748518 38-39 21.620696351292736 27.061077429529274 30.146451833096272 21.17177438608172 40-41 21.587200037662775 27.030752746839333 30.09551884462719 21.286528370870702 42-43 21.24942174304983 27.37517268891111 29.47823095093922 21.897174617099843 44-45 21.606966974844283 27.2434519087801 29.220543100516515 21.929038015859103 46-47 21.86740805490465 27.44427660736696 28.75968306325389 21.928632274474502 48-49 21.865084076836023 27.371525782056956 28.86594255056011 21.89744759054691 50-51 21.527776464410152 27.74986083556658 28.905456233442372 21.81690646658089 52-53 21.780154237842627 27.909115530880452 28.40540981784403 21.90532041343289 54-55 21.39570980712158 27.435498150547105 28.66990238636779 22.498889655963524 56-57 21.6728278630651 27.776488941787203 28.285376026305702 22.265307168841996 58-59 20.950128377725477 27.248026865124412 29.740836211251185 22.061008545898922 60-61 21.114957221647845 27.322838079823285 29.682068362349824 21.880136336179042 62-63 21.830452793807996 27.430899495654465 28.70298758109228 22.035660129445258 64-65 21.580606983051815 27.812615759115207 28.451333083020653 22.155444174812327 66-67 21.297654441379716 27.39063449252019 28.72686292731863 22.584848138781467 68-69 20.648819163053506 27.184089920702842 29.83857878648928 22.328512129754372 70-71 21.493133645277855 27.31521435635488 28.640595246718682 22.551056751648584 72-73 20.86087293335431 27.06420880902381 29.274138797874016 22.800779459747865 74-75 20.942774575939218 27.55642784124391 28.88102565258564 22.619771930231227 76-77 19.953001490887985 27.32069152833787 29.501961338448496 23.224345642325652 78-79 21.006223989718265 27.318020681308624 28.617344263520593 23.058411065452518 80-81 21.101540903551676 27.68105685005795 28.206553466943713 23.010848779446665 82-83 21.268997244514964 27.520748033761304 28.116488854375916 23.09376586734782 84-85 21.288148007686562 27.285153096012515 27.921367084152564 23.505331812148356 86-87 21.16327319154655 27.3720307092699 28.067294123576254 23.3974019756073 88-89 21.346902014492215 27.082387068174977 27.839525188554816 23.73118572877799 90-91 21.004153794034515 27.051193889228784 28.139026112692626 23.805626204044074 92-93 20.751912273469706 27.33947008919811 28.29782343506212 23.61079420227006 94-95 19.866864403851846 27.302843740217085 28.707005250265627 24.123286605665438 96-97 19.332209968715418 27.431577787947926 28.529910756977557 24.706301486359102 98-99 20.18961321903993 26.997368067933643 28.21562190621193 24.597396806814494 100-101 19.37410877237215 26.95509448042261 28.899140008231022 24.771656738974222 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2246.0 1 1684.0 2 1118.5 3 1166.0 4 1198.0 5 1318.5 6 1732.0 7 2510.5 8 3302.5 9 3777.5 10 3853.5 11 3836.0 12 3862.5 13 3826.5 14 3995.0 15 4262.5 16 4499.0 17 4725.0 18 4969.0 19 5382.5 20 5931.0 21 6627.0 22 7573.5 23 8884.0 24 10569.0 25 12581.0 26 15161.0 27 18409.0 28 22012.5 29 26001.5 30 30207.5 31 34612.0 32 39277.0 33 44011.0 34 49324.0 35 54540.5 36 59238.5 37 63282.5 38 66091.5 39 68129.0 40 70088.0 41 72993.0 42 76342.5 43 79289.0 44 82424.5 45 86330.5 46 91082.5 47 99013.5 48 108042.5 49 105846.0 50 96345.5 51 89837.0 52 84111.5 53 78774.0 54 73542.5 55 67129.0 56 59773.5 57 52314.5 58 45065.5 59 37936.5 60 30889.0 61 24233.5 62 18470.0 63 13698.5 64 9935.0 65 7192.0 66 5065.0 67 3583.0 68 2489.5 69 1578.5 70 1037.0 71 710.0 72 474.0 73 325.0 74 204.0 75 125.5 76 82.5 77 41.0 78 20.0 79 10.0 80 7.0 81 6.0 82 4.0 83 1.5 84 1.0 85 1.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025114738235551045 2 0.013817305818555675 3 2.519873401560306E-4 4 0.0 5 0.06581069367074999 6 0.0 7 0.0 8 4.1997890026005096E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.61976790286056E-4 16-17 2.0998945013002548E-5 18-19 4.829757352990586E-4 20-21 0.0 22-23 0.0 24-25 1.259936700780153E-4 26-27 1.259936700780153E-4 28-29 9.659514705981172E-4 30-31 0.0045567710678215525 32-33 0.004955751023068601 34-35 0.001658916656027201 36-37 0.08823756694463669 38-39 0.08097193197013781 40-41 0.08672564290370052 42-43 0.09052645195105398 44-45 0.09445325466848546 46-47 0.08867854478990976 48-49 0.07767509760309642 50-51 0.07108142886901361 52-53 0.060287971132330316 54-55 0.04951551234066001 56-57 0.06988448900327247 58-59 0.6875264586707164 60-61 0.33461818878219557 62-63 0.002750861796703334 64-65 0.002099894501300255 66-67 1.0751039867757044 68-69 2.318304528380494 70-71 2.306125140272953 72-73 1.7645833473326302 74-75 1.2559679001726953 76-77 0.7299233286519685 78-79 0.5618687717129092 80-81 0.0395830113495098 82-83 1.259936700780153E-4 84-85 0.0014069293158711707 86-87 0.001343932480832163 88-89 2.0998945013002548E-5 90-91 0.32842350000335985 92-93 0.055143229604144695 94-95 0.9259904782383733 96-97 1.6254863355665012 98-99 0.6274274780435031 100-101 0.5543931472882803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2381072.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.78313137212133 #Duplication Level Percentage of deduplicated Percentage of total 1 69.25400378311838 32.39919157031015 2 14.970538349043885 14.007373245894012 3 6.137253161320382 8.613597627300553 4 3.0518431713477057 5.710991200490845 5 1.825221300191813 4.269478393503385 6 1.167398131290086 3.2768724083827827 7 0.7675562938083971 2.513608085011576 8 0.5288214367247573 1.979193819735062 9 0.406061699173493 1.7097154035858302 >10 1.75964133196391 13.949626880094204 >50 0.08673718451403246 2.7575954814485724 >100 0.03943805866836259 3.3194571004130875 >500 0.0020685290689214614 0.6826231576966513 >1k 0.0029678895336689953 2.8284850700287882 >5k 2.698081394244541E-4 0.9276252932528775 >10k+ 1.7987209294963608E-4 1.0545652628516407 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14007 0.5882644455942533 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11057 0.4643706700175383 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9085 0.3815508308862563 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7775 0.3265335949521896 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5187 0.21784305556488845 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4489 0.18852852832673686 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3982 0.1672355980835523 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3850 0.1616918766001196 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3528 0.14816855601174597 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3490 0.14657263619075778 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3052 0.12817756035936753 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2602 0.10927850984766525 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2487 0.10444875249467467 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2431 0.10209687065321837 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.0998945013002548E-5 0.0 12-13 0.0 0.0 0.0 1.0499472506501274E-4 0.0 14-15 0.0 0.0 0.0 1.259936700780153E-4 0.0 16-17 0.0 0.0 0.0 2.30988395143028E-4 0.0 18-19 0.0 0.0 0.0 3.149841751950382E-4 0.0 20-21 0.0 0.0 0.0 4.61976790286056E-4 0.0 22-23 0.0 0.0 0.0 0.0012179388107541479 0.0 24-25 0.0 0.0 0.0 0.001994899776235242 0.0 26-27 0.0 0.0 0.0 0.003065845971898372 0.0 28-29 0.0 0.0 0.0 0.008483573785253029 0.0 30-31 0.0 0.0 0.0 0.02274185744908176 0.0 32-33 0.0 0.0 0.0 0.05239236780744136 0.0 34-35 4.1997890026005096E-5 0.0 0.0 0.09699412701505877 0.0 36-37 4.1997890026005096E-5 0.0 0.0 0.16185986816022363 0.0 38-39 4.1997890026005096E-5 2.0998945013002548E-5 0.0 0.2504964150601074 0.0 40-41 4.1997890026005096E-5 4.1997890026005096E-5 0.0 0.354798174939691 0.0 42-43 4.1997890026005096E-5 4.1997890026005096E-5 0.0 0.4584699664688846 0.0 44-45 4.1997890026005096E-5 4.1997890026005096E-5 0.0 0.5815237842450796 0.0 46-47 4.1997890026005096E-5 4.1997890026005096E-5 0.0 0.725534549144251 0.0 48-49 4.1997890026005096E-5 4.1997890026005096E-5 0.0 0.8705322644590336 0.0 50-51 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.0161599481242063 0.0 52-53 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.154479998924854 0.0 54-55 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.3128960401029452 0.0 56-57 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.4661673397528507 0.0 58-59 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.5805695921836886 4.1997890026005096E-5 60-61 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.6778157065389034 4.1997890026005096E-5 62-63 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.7627774380615118 4.1997890026005096E-5 64-65 4.1997890026005096E-5 4.1997890026005096E-5 0.0 1.855949757084204 4.1997890026005096E-5 66-67 4.1997890026005096E-5 6.299683503900764E-5 0.0 1.9401345276413313 4.1997890026005096E-5 68-69 4.1997890026005096E-5 8.399578005201019E-5 0.0 2.0408874658137175 4.1997890026005096E-5 70-71 4.1997890026005096E-5 8.399578005201019E-5 0.0 2.1862001653036955 4.1997890026005096E-5 72-73 4.1997890026005096E-5 8.399578005201019E-5 0.0 2.3778575364373693 4.1997890026005096E-5 74-75 4.1997890026005096E-5 8.399578005201019E-5 0.0 2.6427802267214098 4.1997890026005096E-5 76-77 4.1997890026005096E-5 8.399578005201019E-5 0.0 2.976285471417916 4.1997890026005096E-5 78-79 4.1997890026005096E-5 8.399578005201019E-5 0.0 3.3926735520807436 4.1997890026005096E-5 80-81 4.1997890026005096E-5 8.399578005201019E-5 0.0 3.875670286324815 4.1997890026005096E-5 82-83 4.1997890026005096E-5 1.259936700780153E-4 0.0 4.347474582876956 4.1997890026005096E-5 84-85 4.1997890026005096E-5 1.259936700780153E-4 0.0 4.791560272011934 4.1997890026005096E-5 86-87 4.1997890026005096E-5 1.259936700780153E-4 0.0 5.207108394874242 4.1997890026005096E-5 88-89 4.1997890026005096E-5 1.259936700780153E-4 0.0 5.595945859680009 4.1997890026005096E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10860 0.0 25.162027 1 TACATGG 11030 0.0 24.595308 2 ACATGGG 10790 0.0 23.957247 3 TACCGCG 120 4.671165E-9 22.229076 62-63 GAGTACT 10070 0.0 21.88411 12-13 CATGGGG 3915 0.0 21.326487 4 AGTACTT 10445 0.0 21.030724 12-13 CATGGGA 7320 0.0 19.912474 4 TACGCGG 100 1.0481212E-5 19.45604 64-65 GTACTTT 11405 0.0 19.239824 14-15 GTAGGAC 670 0.0 19.009329 3 ATGGGAG 2650 0.0 18.512508 5 GTATCAA 26640 0.0 17.769026 1 GCTTACG 275 0.0 17.381115 60-61 TACTTTT 12855 0.0 17.124643 14-15 ACTTTTT 13395 0.0 16.645086 16-17 GTCCTAC 660 0.0 16.446775 1 GGTATCA 21295 0.0 16.200806 1 AGAGTAC 21170 0.0 16.009758 10-11 AACGCAG 29540 0.0 15.829558 6 >>END_MODULE