FastQCFastQC Report
Fri 27 May 2016
SRR939661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288835
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95300.2897682614056345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63500.19307748792505552No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49600.15081328190681503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.14348545913674599No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119950.029.7904721
TACATGG119600.029.2829252
ACATGGG121150.027.8502333
GTATCAA170100.025.9454541
CATGGGG57050.022.7917444
CATGGGA73500.022.7913484
ATCAACG197200.022.3799293
CAACGCA198950.022.3738925
TCAACGC198850.022.2419534
TATCAAC198500.022.1619762
AACGCAG202100.022.0452886
GGTATCA123550.021.2021051
GAGTACT82600.020.79655312-13
AGAGTAC139050.020.49265510-11
ACGCAGA220500.019.8826077
AGTACTT89300.019.82075312-13
CGCAGAG221650.019.3524998
GAGTACA92300.018.097711
GTACTTT93050.018.0532914-15
GCAGAGT221450.017.9994749
Produced by FastQC (version 0.11.5)