FastQCFastQC Report
Fri 27 May 2016
SRR939661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288835
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100920.3068563792345922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78580.23892959056930493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52930.1609384478090266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52310.15905328178519143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119250.026.9208721
TACATGG119900.025.8283752
GGTATCA111100.025.8027731
GTATCAA155700.025.4557931
ACATGGG118550.024.6513523
ATCAACG181350.021.5901893
TCAACGC181800.021.588524
CATGGGA73750.021.5039674
CAACGCA184350.021.28995
AACGCAG185400.021.1657096
TATCAAC188100.020.942432
GAGTACT85350.020.86884112-13
ACGCAGA205000.018.844047
AGAGTAC146600.018.7055110-11
CGCAGAG204800.018.6100258
GTACTTT94300.018.56424314-15
AGTACTT91950.018.34870312-13
CATGGGG55750.017.9798244
GAGTACA96350.017.9787391
GTATAGG10350.017.7372951
Produced by FastQC (version 0.11.5)