##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939661_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3288835 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.564536682442263 31.0 31.0 34.0 30.0 34.0 2 31.772987091173622 31.0 31.0 34.0 30.0 34.0 3 31.819208017428664 31.0 31.0 34.0 30.0 34.0 4 35.606046517991935 37.0 35.0 37.0 33.0 37.0 5 35.33603814116549 37.0 35.0 37.0 33.0 37.0 6 35.355652989584456 37.0 35.0 37.0 33.0 37.0 7 35.28388563123416 37.0 35.0 37.0 32.0 37.0 8 35.24972551070516 37.0 35.0 37.0 32.0 37.0 9 36.86408257027185 39.0 37.0 39.0 33.0 39.0 10-11 36.7437730989849 39.0 36.0 39.0 32.0 39.0 12-13 36.68831607544921 39.0 35.0 39.0 32.0 39.0 14-15 37.761771873626984 40.0 37.0 41.0 32.0 41.0 16-17 37.72830272725753 39.0 37.0 41.0 32.0 41.0 18-19 37.70152516012509 39.5 37.0 41.0 32.0 41.0 20-21 37.64090491009735 39.0 36.5 41.0 32.0 41.0 22-23 37.502427911403274 39.0 36.0 41.0 32.0 41.0 24-25 37.376206620277394 39.0 36.0 41.0 31.5 41.0 26-27 37.12479844686644 39.0 36.0 40.5 31.0 41.0 28-29 36.92817395825574 39.0 36.0 40.0 31.0 41.0 30-31 36.70449277631745 38.5 35.5 40.0 30.0 41.0 32-33 36.54620222662432 38.0 35.0 40.0 30.0 41.0 34-35 36.536652948536485 38.0 35.0 40.0 30.0 41.0 36-37 36.443981835513185 38.5 35.0 40.0 30.0 41.0 38-39 36.49327588644611 39.0 35.0 40.0 30.0 41.0 40-41 36.41137272012734 39.0 35.0 40.0 30.0 41.0 42-43 36.235686192831196 38.5 35.0 40.0 30.0 41.0 44-45 36.038785010497634 38.0 35.0 40.0 29.5 41.0 46-47 35.76601775400712 38.0 35.0 40.0 28.5 41.0 48-49 35.49275731376004 38.0 34.0 40.0 27.5 41.0 50-51 35.25551145010316 38.0 34.0 40.0 27.0 41.0 52-53 35.029816485168766 38.0 34.0 40.0 26.5 41.0 54-55 34.74735917125669 38.0 34.0 40.0 26.0 41.0 56-57 32.73188150211245 37.0 30.5 39.5 16.5 41.0 58-59 27.696966250967286 33.0 18.0 38.5 7.0 40.0 60-61 28.45233403317588 33.0 20.5 38.0 9.5 40.0 62-63 31.52976737963443 35.0 29.5 38.5 15.0 40.0 64-65 31.756828633847547 35.5 31.0 39.0 10.5 40.0 66-67 25.040887882791324 28.0 18.0 35.5 2.0 39.0 68-69 17.52229695317643 13.0 7.5 28.0 2.0 35.5 70-71 16.377996919881966 13.0 7.5 27.0 2.0 33.5 72-73 17.518134232942668 13.5 7.0 28.5 2.0 34.0 74-75 18.53259512867018 17.5 9.0 30.0 2.0 34.5 76-77 19.74675485392244 21.5 8.0 30.0 2.0 34.5 78-79 22.871248633634707 27.5 10.5 33.5 2.0 35.5 80-81 26.519412497130443 31.5 23.5 34.5 2.0 36.0 82-83 27.828926808429124 33.5 27.5 35.0 2.0 36.0 84-85 27.885528462206224 34.0 28.0 35.0 2.0 36.0 86-87 27.65199075052412 34.0 27.0 35.0 2.0 36.0 88-89 27.24287338829707 33.5 26.5 35.0 2.0 35.0 90-91 25.917179639598825 32.5 22.0 35.0 2.0 35.0 92-93 23.44905430038296 31.5 2.0 34.5 2.0 35.0 94-95 16.06234015996546 7.5 2.0 32.0 2.0 35.0 96-97 13.461625469201099 2.0 2.0 30.0 2.0 34.0 98-99 13.152809733537865 2.0 2.0 30.0 2.0 34.0 100-101 12.859181138609873 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.34974069242552375 1101 2 0.32102377880617894 1101 3 0.356175809652612 1101 4 0.22397101766691918 1101 5 0.27420635747625255 1101 6 0.40448376356421534 1101 7 0.391049045017958 1101 8 0.4125400823276948 1101 9 0.4616001216083063 1101 10-11 0.5228502326382127 1101 12-13 0.4536575870845141 1101 14-15 0.6506084430477372 1101 16-17 0.6299339966372344 1101 18-19 0.613833332792737 1101 20-21 0.5427531432953927 1101 22-23 0.5672746470669665 1101 24-25 0.48603227767714685 1101 26-27 0.5082732591366863 1101 28-29 0.6082178811283754 1101 30-31 0.5496035047603272 1101 32-33 0.5982852404347767 1101 34-35 0.3700043834984683 1101 36-37 0.2396393723968444 1101 38-39 0.2477570311669126 1101 40-41 0.491195388269837 1101 42-43 0.5332781814886047 1101 44-45 0.43372985028093325 1101 46-47 0.31016985071173764 1101 48-49 0.46280061127013283 1101 50-51 0.5010286061440752 1101 52-53 0.3725134765188898 1101 54-55 0.22453455076912832 1101 56-57 -2.1165936425878087 1101 58-59 -9.911150266685098 1101 60-61 -10.435677106769763 1101 62-63 -4.122895105590342 1101 64-65 -4.902850824273344 1101 66-67 -9.633168265038904 1101 68-69 -4.854702289539977 1101 70-71 -4.493918213683937 1101 72-73 -4.559545388301476 1101 74-75 -6.322716314079823 1101 76-77 -7.923523137814531 1101 78-79 -11.156362873489424 1101 80-81 -7.907760697511687 1101 82-83 -4.060160788763376 1101 84-85 -3.1797624943210714 1101 86-87 -3.058734168260944 1101 88-89 -3.19898530200841 1101 90-91 -9.670122615153712 1101 92-93 -17.031521159487028 1101 94-95 -11.145646655883672 1101 96-97 -9.21486216608214 1101 98-99 -9.249034317849821 1101 100-101 -9.066888795791463 1102 1 0.38847454270374016 1102 2 0.3636422932510541 1102 3 0.3335711279114193 1102 4 0.2637151622716445 1102 5 0.3310681040690113 1102 6 0.23960743107344484 1102 7 0.3974801658921052 1102 8 0.38604108008219384 1102 9 0.4234295576929341 1102 10-11 0.3765541019997656 1102 12-13 0.33223624928744755 1102 14-15 0.4437355581377318 1102 16-17 0.46862628442881515 1102 18-19 0.4817940470559421 1102 20-21 0.53656436967308 1102 22-23 0.39541423425585265 1102 24-25 0.4038265341143372 1102 26-27 0.43149739493805583 1102 28-29 0.46663397620467606 1102 30-31 0.4123491931720338 1102 32-33 0.4309999199322121 1102 34-35 0.27880577315274735 1102 36-37 0.3134152002500912 1102 38-39 0.32679931828121056 1102 40-41 0.31012658458411835 1102 42-43 0.3681873307746457 1102 44-45 0.4538400451873983 1102 46-47 0.4550041191227834 1102 48-49 0.3778497854990306 1102 50-51 0.45084227235640384 1102 52-53 0.4374922466582234 1102 54-55 0.33091600856228354 1102 56-57 -2.3672339449491986 1102 58-59 -9.95611976405797 1102 60-61 -10.26888734415071 1102 62-63 -3.939197095748117 1102 64-65 -4.602356728252065 1102 66-67 -6.531657624894802 1102 68-69 -4.238104694745923 1102 70-71 -4.226608457282488 1102 72-73 -4.510303497982184 1102 74-75 -5.146633131168539 1102 76-77 -7.362237983883791 1102 78-79 -10.012586912861533 1102 80-81 -5.5915989254235186 1102 82-83 -2.962692197678024 1102 84-85 -2.563033667216054 1102 86-87 -2.5276190342718827 1102 88-89 -2.5783064963418028 1102 90-91 -8.61886898782424 1102 92-93 -13.063095975382915 1102 94-95 -10.338667323262673 1102 96-97 -9.265219765747279 1102 98-99 -9.334262226865125 1102 100-101 -9.29692401594188 1103 1 0.2048274922607689 1103 2 0.16433146078592387 1103 3 0.17291397361358918 1103 4 0.20925068353459153 1103 5 0.2533353037657875 1103 6 0.19192989506294822 1103 7 0.17645308108483704 1103 8 0.15849131373519043 1103 9 0.25078077662868026 1103 10-11 0.35650473188946563 1103 12-13 0.32899269135175047 1103 14-15 0.4122926124484749 1103 16-17 0.2886800278756638 1103 18-19 0.3701143332685888 1103 20-21 0.3482740351293714 1103 22-23 0.3643484500726757 1103 24-25 0.33050149874768664 1103 26-27 0.37429125808198194 1103 28-29 0.36054045028325277 1103 30-31 0.2484434148985457 1103 32-33 0.20577024480701311 1103 34-35 0.2504225088261123 1103 36-37 0.1989038802871761 1103 38-39 0.23512963525299568 1103 40-41 0.2862700337446995 1103 42-43 0.22124875423468637 1103 44-45 0.14453416400092323 1103 46-47 0.20899659801781212 1103 48-49 0.1234660325580208 1103 50-51 0.13770755053524653 1103 52-53 0.11826840326874333 1103 54-55 -0.020684999641702007 1103 56-57 -2.9683651813348604 1103 58-59 -10.480225791518595 1103 60-61 -10.752493121876238 1103 62-63 -4.685001096451575 1103 64-65 -6.0172630235718 1103 66-67 -7.057934295398216 1103 68-69 -4.221522873909837 1103 70-71 -4.227299299712207 1103 72-73 -4.675960937115523 1103 74-75 -5.268101519751173 1103 76-77 -6.90206610452047 1103 78-79 -9.362363112791869 1103 80-81 -5.375649615185537 1103 82-83 -3.2613079074266054 1103 84-85 -2.8039525579518063 1103 86-87 -2.598701209679067 1103 88-89 -2.605101970693216 1103 90-91 -5.548308149285031 1103 92-93 -10.444439083898834 1103 94-95 -8.489847829471724 1103 96-97 -8.079646224371329 1103 98-99 -8.402371670697683 1103 100-101 -8.423018681205356 1104 1 0.23299573653202543 1104 2 0.23947311509394353 1104 3 0.1646253600467631 1104 4 0.1720654299232578 1104 5 0.20083993944208345 1104 6 0.17124691850013818 1104 7 0.25309211362805684 1104 8 0.23646835411597067 1104 9 0.26059084986600567 1104 10-11 0.3018527262170707 1104 12-13 0.2997061485177781 1104 14-15 0.38571333703991684 1104 16-17 0.33662954777572196 1104 18-19 0.35851316945881706 1104 20-21 0.34694671511983444 1104 22-23 0.41511076278877823 1104 24-25 0.3990499807649499 1104 26-27 0.5258788875839642 1104 28-29 0.42805180711519597 1104 30-31 0.42345565178518996 1104 32-33 0.38372204026741485 1104 34-35 0.45174452550832456 1104 36-37 0.37905006271676456 1104 38-39 0.3371438714399275 1104 40-41 0.22796779657936384 1104 42-43 0.35487731514164267 1104 44-45 0.3692241019925788 1104 46-47 0.4560357314324861 1104 48-49 0.3258009577012828 1104 50-51 0.31336863328527187 1104 52-53 0.3978913421075845 1104 54-55 0.1444733303838106 1104 56-57 -2.9456973804055337 1104 58-59 -10.205983294712091 1104 60-61 -10.509120860496882 1104 62-63 -4.204542234172219 1104 64-65 -5.375563258460495 1104 66-67 -6.6828961100885955 1104 68-69 -4.184812619845285 1104 70-71 -4.1071752830262085 1104 72-73 -4.441557386916688 1104 74-75 -5.212056308344581 1104 76-77 -6.9233626165275 1104 78-79 -9.398871109857723 1104 80-81 -4.967154334199833 1104 82-83 -2.763021612712592 1104 84-85 -2.2955822298517212 1104 86-87 -2.272918353334031 1104 88-89 -2.205146534877411 1104 90-91 -4.913999084897501 1104 92-93 -7.2488496664179305 1104 94-95 -8.199950235098878 1104 96-97 -8.205150277222774 1104 98-99 -8.495124565678543 1104 100-101 -8.542203792492703 1105 1 0.2462181612079597 1105 2 0.27061654212368325 1105 3 0.2242527419928706 1105 4 0.23227893970539526 1105 5 0.20789665317150252 1105 6 0.1937657287800647 1105 7 0.17108261083698295 1105 8 0.2365420513970946 1105 9 0.3190417294780019 1105 10-11 0.35504557466121867 1105 12-13 0.31254327708413854 1105 14-15 0.4444340124007482 1105 16-17 0.41140800251272225 1105 18-19 0.4280210299860556 1105 20-21 0.37873682915598295 1105 22-23 0.46536173609275977 1105 24-25 0.5101382808128534 1105 26-27 0.6103633981052852 1105 28-29 0.5531940813531264 1105 30-31 0.5079085777574619 1105 32-33 0.4479623588732409 1105 34-35 0.4741500029946053 1105 36-37 0.3671846677484396 1105 38-39 0.38691572394450446 1105 40-41 0.3201616797050306 1105 42-43 0.3246438239297973 1105 44-45 0.27372804099281467 1105 46-47 0.2760056155207309 1105 48-49 0.08606547166417045 1105 50-51 0.18497469875730133 1105 52-53 0.2510445108031405 1105 54-55 0.10979296469312771 1105 56-57 1.4290078194109697 1105 58-59 -8.825699175326037 1105 60-61 -9.919587953510725 1105 62-63 -3.862357441557517 1105 64-65 -5.1201032675465825 1105 66-67 -6.717274608884711 1105 68-69 -4.189915559375914 1105 70-71 -4.095381152355532 1105 72-73 -2.407616117368219 1105 74-75 -4.400983004322063 1105 76-77 -6.604629705471625 1105 78-79 -9.277360660636077 1105 80-81 -4.8374474733191555 1105 82-83 -2.453158961264897 1105 84-85 -1.8560761520378328 1105 86-87 -1.6458715396355394 1105 88-89 -1.8418179657680724 1105 90-91 -4.430607116662532 1105 92-93 -6.949967732912697 1105 94-95 -7.971060104651153 1105 96-97 -8.258421423498607 1105 98-99 -8.57288643321896 1105 100-101 -8.517631455691705 1106 1 0.12154257920851563 1106 2 0.1907497980972579 1106 3 0.10858255621569057 1106 4 0.07538861232952598 1106 5 0.004387212626646431 1106 6 0.15640883485015422 1106 7 0.17621271933025184 1106 8 0.17904905738488708 1106 9 0.18037246898418857 1106 10-11 0.2484051496376125 1106 12-13 0.2068632601711755 1106 14-15 0.2727378238856346 1106 16-17 0.21948773615245187 1106 18-19 0.15408174033064626 1106 20-21 0.21399647732077653 1106 22-23 0.1731919939965394 1106 24-25 0.2737382872594978 1106 26-27 0.22937863494369282 1106 28-29 0.33438755469245507 1106 30-31 0.21865290944879234 1106 32-33 0.08756633569564798 1106 34-35 0.1685823757776177 1106 36-37 0.18277166334575412 1106 38-39 0.2425538850575748 1106 40-41 0.19254562110880613 1106 42-43 0.16600266323007418 1106 44-45 0.08621135555502946 1106 46-47 0.17287202394232537 1106 48-49 0.18468579452739675 1106 50-51 0.19088200735826888 1106 52-53 0.30826480219155883 1106 54-55 0.22757172526846858 1106 56-57 1.3521167715275553 1106 58-59 -9.194317658998955 1106 60-61 -10.492237155453822 1106 62-63 -4.121646348016679 1106 64-65 -5.596020350401158 1106 66-67 -7.181089250735578 1106 68-69 -3.9853301925048505 1106 70-71 -4.196504290886379 1106 72-73 -4.68988945138414 1106 74-75 -5.129394086107254 1106 76-77 -6.818718709009698 1106 78-79 -9.567555216632787 1106 80-81 -5.0684932426126394 1106 82-83 -2.729361829643338 1106 84-85 -2.209655510255221 1106 86-87 -2.1795213274650287 1106 88-89 -2.0144964620309125 1106 90-91 -4.73932369364497 1106 92-93 -6.987501530404572 1106 94-95 -7.6682472811337865 1106 96-97 -7.554013339403999 1106 98-99 -7.106799632917105 1106 100-101 -7.525390753083089 1107 1 0.09027432156477033 1107 2 -0.01093018210785246 1107 3 -0.06912520699905755 1107 4 0.052238751004523465 1107 5 -0.03128365306726977 1107 6 -0.11844510399989616 1107 7 0.01980957209100609 1107 8 0.12083925743880997 1107 9 0.03811924954531776 1107 10-11 0.03487962863693639 1107 12-13 -0.09503179553887264 1107 14-15 0.09743637656920612 1107 16-17 0.07320407838241039 1107 18-19 0.0367401178885558 1107 20-21 0.001563275830250177 1107 22-23 0.12989423421635138 1107 24-25 0.2634021527281334 1107 26-27 0.24089318381601998 1107 28-29 0.15038692871608816 1107 30-31 0.2445875807835165 1107 32-33 0.14905489454665855 1107 34-35 0.09879099763250565 1107 36-37 -0.08755913469099852 1107 38-39 -0.11744511781194689 1107 40-41 -0.055063435421104145 1107 42-43 -0.14918788101851277 1107 44-45 -0.047226537988358075 1107 46-47 -0.04628483760795632 1107 48-49 -0.32701359279132447 1107 50-51 -0.3701502258077696 1107 52-53 -0.3106302202808138 1107 54-55 -0.4175461669454137 1107 56-57 0.863708967319667 1107 58-59 -9.093912897885566 1107 60-61 -10.856914777630003 1107 62-63 -4.384518979370153 1107 64-65 -5.787924620335456 1107 66-67 -7.315120252260822 1107 68-69 -4.30570941599872 1107 70-71 -4.21953828195652 1107 72-73 -2.563480358292775 1107 74-75 -4.866803790402669 1107 76-77 -6.854930471825593 1107 78-79 -7.686881359547435 1107 80-81 -2.9039292748458045 1107 82-83 -1.6651526279755444 1107 84-85 -1.5112625060488085 1107 86-87 -1.3798309918752238 1107 88-89 -1.2974235108570724 1107 90-91 -0.8787572035073303 1107 92-93 -3.634748803991325 1107 94-95 -6.01613114867054 1107 96-97 -6.582507173813864 1107 98-99 -6.292932548563559 1107 100-101 -7.015528578673888 1108 1 -0.1662203506517379 1108 2 -0.08739652102960349 1108 3 -0.16980213139841993 1108 4 -0.12107918691349084 1108 5 -0.09796343393882267 1108 6 -0.1886972934659923 1108 7 -0.16355511411030932 1108 8 -0.1411015567218925 1108 9 -0.20139024062068245 1108 10-11 -0.18656589837226534 1108 12-13 -0.17785659527174147 1108 14-15 -0.27149510777144314 1108 16-17 -0.23387536063818715 1108 18-19 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84-85 1.4726763836076842 2316 86-87 1.3834086111618689 2316 88-89 1.4426102935120646 2316 90-91 2.482764058315947 2316 92-93 4.576705228139534 2316 94-95 11.475685292056264 2316 96-97 13.458490385212338 2316 98-99 13.21115947561875 2316 100-101 12.164649890787857 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 24.0 4 38.0 5 114.0 6 247.0 7 696.0 8 1540.0 9 2612.0 10 4374.0 11 6866.0 12 10620.0 13 14514.0 14 18065.0 15 25172.0 16 34351.0 17 45758.0 18 51681.0 19 52736.0 20 55339.0 21 55700.0 22 55546.0 23 47126.0 24 47889.0 25 57230.0 26 81103.0 27 120801.0 28 171163.0 29 231952.0 30 289152.0 31 318509.0 32 321568.0 33 295196.0 34 263361.0 35 237737.0 36 193968.0 37 125610.0 38 47379.0 39 3090.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44149374564719 19.71801430000821 13.969392751459045 23.87109920288555 2 15.496009253763294 21.299802226352305 39.2459362745575 23.958252245326896 3 18.809698281759772 25.575934298823594 30.36696940857387 25.247398010842765 4 12.83685560388405 17.232333029781064 37.482087122035615 32.44872424429927 5 13.216827611825549 38.19002540918841 34.77039624023177 13.822750738754264 6 31.39561577275844 37.17766321508984 17.56828785877066 13.85843315338106 7 27.629974635387494 33.05852469294589 21.77403906673307 17.53746160493354 8 25.860724799759673 35.49898777608111 20.169952669637965 18.470334754521257 9 26.26589658648123 16.313983523040836 20.41692575030368 37.00319414017426 10-11 24.545956242864115 26.629703223177813 28.22481212952307 20.599528404435006 12-13 25.612792979884976 24.136860012740076 28.346587773482103 21.903759233892853 14-15 22.536817223473264 25.317971767330107 26.449656319877015 25.695554689319618 16-17 22.05571273718505 28.575574633570856 27.005428974089607 22.363283655154483 18-19 22.02655501610374 27.489405434907372 28.91243321148158 21.57160633750731 20-21 22.76435272672542 26.65535060287305 28.714286365840792 21.866010304560735 22-23 22.538562778718422 26.66628253764148 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22.414583476017626 48-49 22.556738624879767 27.08490095091987 27.88085497558778 22.477505448612582 50-51 22.242026137921904 27.216419375605927 28.02385804493702 22.517696441535158 52-53 22.594820814976195 27.356913243949034 27.60111882033901 22.44714712073576 54-55 22.374707598446612 27.023844752366788 27.75828898972648 22.843158659460116 56-57 21.513616556485264 27.244929859198635 28.70615769966605 22.535295884650044 58-59 21.071954087573463 26.809339344733495 29.708474069465744 22.410232498227302 60-61 22.247269275227836 26.995466162485844 28.432714660426754 22.324549901859562 62-63 22.37749798476663 27.10919904241827 27.96474960782388 22.54855336499122 64-65 21.89594902020117 27.197724503174975 28.23388714877642 22.67243932784744 66-67 21.560436147788902 27.002179747164206 28.755771309097323 22.68161279594957 68-69 21.578323558582934 26.542476895356913 29.327537442056475 22.55166210400368 70-71 21.542944530603457 26.58641292086037 29.206915103778307 22.66372744475786 72-73 20.79981080650751 26.618025831467936 29.75553141205156 22.826631949972995 74-75 21.351146845132707 26.961152798619807 28.883068115152042 22.80463224109544 76-77 19.79064177459691 27.25513261322788 29.522797146037455 23.431428466137756 78-79 20.61670658280135 27.215483592949347 28.873207879116574 23.294601945132733 80-81 21.880749742022157 27.1331999230934 27.745501815579914 23.24054851930453 82-83 22.170168877840034 27.116309249467974 27.472576088678423 23.24094578401357 84-85 22.067143361230297 27.05256598208146 27.424658962910346 23.45563169377789 86-87 21.965573382441097 27.06835868106554 27.555189602111273 23.410878334382087 88-89 22.032810133325466 26.93561527855714 27.372092489384148 23.659482098733243 90-91 21.36603479464502 26.99879307399132 27.959731132427017 23.675440998936644 92-93 20.478452293429697 27.172303356372968 28.62461372735191 23.724630622845424 94-95 19.39497443319086 27.046542993577464 29.262250391083377 24.296232182148294 96-97 19.43008446487791 27.156275369491972 28.79442694139368 24.619213224236436 98-99 19.756142126813803 27.05869473203774 28.465314436339835 24.719848704808616 100-101 20.174228182686978 26.94095588538854 28.21663510503585 24.668180826888637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 415.0 1 328.5 2 279.5 3 341.0 4 409.0 5 484.5 6 610.0 7 864.0 8 1199.5 9 1501.0 10 1693.5 11 1919.5 12 2215.0 13 2524.0 14 2791.0 15 3074.0 16 3421.0 17 3761.0 18 4144.0 19 4688.0 20 5313.0 21 6103.5 22 7197.5 23 8666.0 24 10723.0 25 13464.5 26 17051.0 27 21388.5 28 26242.5 29 31811.0 30 38247.5 31 45173.0 32 52770.5 33 60720.5 34 68787.0 35 76828.0 36 84555.5 37 91444.0 38 96620.5 39 100539.0 40 105135.5 41 109988.0 42 113833.5 43 118529.5 44 122951.5 45 126397.5 46 129815.0 47 132697.5 48 135234.5 49 134838.0 50 130721.0 51 125435.5 52 119668.0 53 113716.5 54 106863.0 55 98166.0 56 88659.0 57 78579.5 58 68087.0 59 57415.5 60 46672.0 61 36362.0 62 27130.5 63 19724.5 64 14004.0 65 9684.0 66 6485.5 67 4301.0 68 2802.5 69 1762.5 70 1109.5 71 724.5 72 482.0 73 325.5 74 214.5 75 109.5 76 53.0 77 26.0 78 11.0 79 6.5 80 4.5 81 3.0 82 1.0 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0220442801174276 2 0.006902140119525607 3 1.5202951805122483E-4 4 0.0 5 0.1395630975710244 6 0.0 7 3.0405903610244966E-5 8 0.0011250184335790637 9 0.0 10-11 0.0 12-13 0.0 14-15 6.385239758151443E-4 16-17 0.0 18-19 7.601475902561242E-5 20-21 0.0 22-23 2.8885608429732714E-4 24-25 1.5202951805122483E-5 26-27 2.4324722888195973E-4 28-29 0.0032078228308808437 30-31 0.012846494275328498 32-33 0.0026909224695066797 34-35 0.00446966783070601 36-37 0.10687675119001105 38-39 0.10587335637087297 40-41 0.11805092076677608 42-43 0.12093948160974935 44-45 0.11681948167056115 46-47 0.10976531203298431 48-49 0.10930922347883065 50-51 0.10854907588857451 52-53 0.08738656697584403 54-55 0.07517859667633067 56-57 0.550118811068357 58-59 1.567165880927441 60-61 0.5166875200488927 62-63 0.004074391083772826 64-65 0.11937357757382173 66-67 1.8947134775688046 68-69 2.848972356472733 70-71 2.7650064536530414 72-73 2.6077623231326594 74-75 1.9920123691215887 76-77 1.4021834479382518 78-79 0.9351031596294737 80-81 0.05263261914933403 82-83 2.4324722888195973E-4 84-85 1.9763837346659227E-4 86-87 4.10479698738307E-4 88-89 1.0642066263585739E-4 90-91 0.24891792990527042 92-93 0.38960604590987385 94-95 1.9890781994231999 96-97 2.414061514183594 98-99 1.4037645549259843 100-101 0.9402569602914101 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3288835.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.55376993849806 #Duplication Level Percentage of deduplicated Percentage of total 1 68.03594970469018 30.312580506899913 2 15.091553767106708 13.447712291082919 3 6.244025319391761 8.34584602711012 4 3.2152930576936094 5.730137087093241 5 1.8737709076380176 4.1741778968177465 6 1.258546991475717 3.3643807868998787 7 0.8396433848046503 2.618649473787773 8 0.5837996816750014 2.080838136601313 9 0.45999266790789767 1.8444966749427971 >10 2.2082144156113044 17.187731958627815 >50 0.1255686858502217 3.8208083080104878 >100 0.05920438767999475 4.660593714138601 >500 0.0032765748751392675 0.924442180069526 >1k 8.874056940967975E-4 0.6216171106839522 >5k 2.0478592940695323E-4 0.5590569855957928 >10k+ 6.826197646898441E-5 0.3069308616381646 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10092 0.3068563792345922 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7858 0.23892959056930493 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5293 0.1609384478090266 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5231 0.15905328178519143 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.0405903610244966E-5 0.0 0.0 0.0 0.0 3 3.0405903610244966E-5 0.0 0.0 0.0 0.0 4 3.0405903610244966E-5 0.0 0.0 0.0 0.0 5 3.0405903610244966E-5 0.0 0.0 0.0 0.0 6 6.081180722048993E-5 0.0 0.0 0.0 3.0405903610244966E-5 7 9.12177108307349E-5 0.0 0.0 0.0 3.0405903610244966E-5 8 1.5202951805122483E-4 0.0 0.0 0.0 3.0405903610244966E-5 9 1.5202951805122483E-4 0.0 0.0 0.0 6.081180722048993E-5 10-11 1.5202951805122483E-4 0.0 0.0 0.0 6.081180722048993E-5 12-13 1.5202951805122483E-4 0.0 0.0 0.0 6.081180722048993E-5 14-15 1.824354216614698E-4 0.0 0.0 4.560885541536745E-5 6.081180722048993E-5 16-17 1.824354216614698E-4 0.0 0.0 1.3682656624610236E-4 9.12177108307349E-5 18-19 3.1926198790757215E-4 0.0 0.0 2.5845018068708223E-4 1.2162361444097986E-4 20-21 3.648708433229396E-4 0.0 0.0 5.016974095690419E-4 1.2162361444097986E-4 22-23 3.648708433229396E-4 0.0 0.0 0.0010946125299688188 1.5202951805122483E-4 24-25 5.169003613741644E-4 0.0 0.0 0.0018699630720300654 1.5202951805122483E-4 26-27 5.321033131792869E-4 0.0 0.0 0.0023412545779888626 1.5202951805122483E-4 28-29 6.689298794253892E-4 0.0 0.0 0.004682509155977725 1.5202951805122483E-4 30-31 6.689298794253892E-4 0.0 0.0 0.012207970299513354 1.5202951805122483E-4 32-33 6.689298794253892E-4 0.0 0.0 0.028186272646697083 3.0405903610244967E-4 34-35 6.993357830356342E-4 0.0 0.0 0.05197889222171377 3.0405903610244967E-4 36-37 7.297416866458792E-4 0.0 0.0 0.0869000725180801 3.344649397126946E-4 38-39 8.51365301086859E-4 0.0 0.0 0.1411137986551469 3.344649397126946E-4 40-41 8.51365301086859E-4 0.0 0.0 0.19931069816515573 3.344649397126946E-4 42-43 8.81771204697104E-4 0.0 0.0 0.26234213634919357 3.344649397126946E-4 44-45 9.42583011917594E-4 0.0 0.0 0.336867006097904 3.344649397126946E-4 46-47 0.0010946125299688188 0.0 0.0 0.42255084247157426 3.344649397126946E-4 48-49 0.0011250184335790637 0.0 0.0 0.5014997711955753 3.344649397126946E-4 50-51 0.0011250184335790637 0.0 0.0 0.5806159323894328 3.344649397126946E-4 52-53 0.0011250184335790637 0.0 0.0 0.6612827946674127 3.344649397126946E-4 54-55 0.0011250184335790637 0.0 0.0 0.7481828671854927 3.344649397126946E-4 56-57 0.0011554243371893088 0.0 0.0 0.8208681797657833 3.496678915178171E-4 58-59 0.0011554243371893088 0.0 0.0 0.8707186587347799 3.648708433229396E-4 60-61 0.0011554243371893088 3.0405903610244966E-5 0.0 0.9100791009582421 3.648708433229396E-4 62-63 0.0011554243371893088 3.0405903610244966E-5 0.0 0.9481016834228534 3.9527674693318453E-4 64-65 0.0011554243371893088 3.0405903610244966E-5 0.0 0.9834333434179581 3.9527674693318453E-4 66-67 0.0011554243371893088 3.0405903610244966E-5 0.0 1.015557180582182 3.9527674693318453E-4 68-69 0.0011554243371893088 3.0405903610244966E-5 0.0 1.0604514972627084 3.9527674693318453E-4 70-71 0.0011554243371893088 3.0405903610244966E-5 1.5202951805122483E-5 1.129913784060313 3.9527674693318453E-4 72-73 0.0011554243371893088 3.0405903610244966E-5 3.0405903610244966E-5 1.2276687641672508 4.256826505434295E-4 74-75 0.0011554243371893088 3.0405903610244966E-5 3.0405903610244966E-5 1.3526978398125782 4.256826505434295E-4 76-77 0.0011554243371893088 3.0405903610244966E-5 3.0405903610244966E-5 1.5523582058692516 4.40885602348552E-4 78-79 0.0011554243371893088 3.0405903610244966E-5 3.0405903610244966E-5 1.863821079500796 4.560885541536745E-4 80-81 0.0013074538552405334 3.0405903610244966E-5 3.0405903610244966E-5 2.2320821810762776 4.71291505958797E-4 82-83 0.0013074538552405334 3.0405903610244966E-5 3.0405903610244966E-5 2.5954631351223156 4.8649445776391946E-4 84-85 0.0013074538552405334 3.0405903610244966E-5 3.0405903610244966E-5 2.8796367102636644 4.8649445776391946E-4 86-87 0.0013074538552405334 4.560885541536745E-5 3.0405903610244966E-5 3.096978109269696 4.8649445776391946E-4 88-89 0.0013074538552405334 6.081180722048993E-5 3.0405903610244966E-5 3.309469766649893 4.8649445776391946E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11925 0.0 26.920872 1 TACATGG 11990 0.0 25.828375 2 GGTATCA 11110 0.0 25.802773 1 GTATCAA 15570 0.0 25.455793 1 ACATGGG 11855 0.0 24.651352 3 ATCAACG 18135 0.0 21.590189 3 TCAACGC 18180 0.0 21.58852 4 CATGGGA 7375 0.0 21.503967 4 CAACGCA 18435 0.0 21.2899 5 AACGCAG 18540 0.0 21.165709 6 TATCAAC 18810 0.0 20.94243 2 GAGTACT 8535 0.0 20.868841 12-13 ACGCAGA 20500 0.0 18.84404 7 AGAGTAC 14660 0.0 18.70551 10-11 CGCAGAG 20480 0.0 18.610025 8 GTACTTT 9430 0.0 18.564243 14-15 AGTACTT 9195 0.0 18.348703 12-13 CATGGGG 5575 0.0 17.979824 4 GAGTACA 9635 0.0 17.978739 1 GTATAGG 1035 0.0 17.737295 1 >>END_MODULE