FastQCFastQC Report
Fri 27 May 2016
SRR939660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262876
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.12096690159233756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG98300.033.851071
GTATCAA142750.030.6038421
TACATGG118550.027.3369162
ACATGGG114050.026.8419063
GGTATCA107300.024.352781
TATCAAC183450.024.052272
ATCAACG192400.022.9092143
TCAACGC194900.022.66074
CAACGCA195750.022.4936475
AACGCAG200350.022.166326
CATGGGG55950.021.612924
CATGGGA71900.021.5485744
GAGTACT80450.021.35315312-13
ACGCAGA205700.021.107997
AGAGTAC142300.019.60059710-11
CGCAGAG218000.019.5700518
AGTACTT89300.019.26339112-13
GTACTTT89250.019.01198214-15
GCAGAGT213400.018.7537529
ATGGGAG37900.017.6819085
Produced by FastQC (version 0.11.5)