FastQCFastQC Report
Fri 27 May 2016
SRR939660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262876
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98580.30212609979662114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77150.23644784539774114No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51170.15682483796503452No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.15452625230011804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111750.026.1311111
GTATCAA161100.026.0035231
TACATGG110450.025.4077432
GGTATCA115450.023.6183011
ACATGGG113750.023.58973
TCAACGC189600.021.9874824
CAACGCA190350.021.8760745
ATCAACG190600.021.8721243
AACGCAG191550.021.7991816
TATCAAC195400.021.4340022
GAGTACT88100.020.72960912-13
CATGGGA69600.019.988234
AGAGTAC144300.019.57994710-11
CATGGGG52850.019.4523944
ACGCAGA211900.019.4165447
CGCAGAG210450.019.3935628
AGTACTT94050.018.94210612-13
GTACTTT96350.018.68559514-15
GCAGAGT217450.017.8589739
CGCATAG4100.017.80831766-67
Produced by FastQC (version 0.11.5)