##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939660_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3262876 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48399540773232 31.0 31.0 34.0 30.0 34.0 2 31.688249875263416 31.0 31.0 34.0 30.0 34.0 3 31.742927405148098 31.0 31.0 34.0 30.0 34.0 4 35.54534772390983 37.0 35.0 37.0 33.0 37.0 5 35.29729723103176 37.0 35.0 37.0 33.0 37.0 6 35.29835304804718 37.0 35.0 37.0 32.0 37.0 7 35.20465564734915 37.0 35.0 37.0 32.0 37.0 8 35.16025248890856 37.0 35.0 37.0 32.0 37.0 9 36.7670711360162 39.0 35.0 39.0 32.0 39.0 10-11 36.64401635244489 39.0 35.0 39.0 32.0 39.0 12-13 36.579021390944675 39.0 35.0 39.0 32.0 39.0 14-15 37.65954268565523 39.0 36.0 41.0 32.0 41.0 16-17 37.609618784164645 39.0 36.0 41.0 32.0 41.0 18-19 37.57586696521719 39.0 36.0 41.0 32.0 41.0 20-21 37.51391333902974 39.0 36.0 41.0 32.0 41.0 22-23 37.37980542319107 39.0 36.0 41.0 32.0 41.0 24-25 37.249438685380625 39.0 36.0 40.5 31.0 41.0 26-27 36.972213010853004 39.0 36.0 40.0 31.0 41.0 28-29 36.77767543112273 39.0 36.0 40.0 30.0 41.0 30-31 36.54513793965814 38.0 35.0 40.0 30.0 41.0 32-33 36.387424315236004 38.0 35.0 40.0 30.0 41.0 34-35 36.36375945638143 38.0 35.0 40.0 30.0 41.0 36-37 36.29922880918552 38.0 35.0 40.0 30.0 41.0 38-39 36.35971547800162 38.0 35.0 40.0 30.0 41.0 40-41 36.261434391009644 38.0 35.0 40.0 30.0 41.0 42-43 36.094265457835355 38.0 35.0 40.0 29.5 41.0 44-45 35.892923298341714 38.0 35.0 40.0 29.0 41.0 46-47 35.62752691184097 38.0 34.5 40.0 27.5 41.0 48-49 35.356750608971964 38.0 34.0 40.0 27.0 41.0 50-51 35.13031831427244 38.0 34.0 40.0 26.5 41.0 52-53 34.90767868592003 38.0 34.0 40.0 26.0 41.0 54-55 34.628204534894984 38.0 33.0 40.0 26.0 41.0 56-57 33.90268079448928 37.5 32.5 40.0 23.5 41.0 58-59 30.895191542675846 35.5 28.0 39.0 8.5 40.5 60-61 30.62049186055492 34.5 27.0 39.0 10.5 40.0 62-63 32.325400965283386 35.5 30.5 39.0 20.5 40.0 64-65 32.35406386880776 36.0 31.0 39.0 19.0 40.0 66-67 27.566301171114073 32.0 19.0 37.0 5.0 39.5 68-69 20.006694860607634 20.5 8.0 32.5 2.0 37.0 70-71 18.036752852391572 16.0 7.5 29.0 2.0 35.0 72-73 19.79451992659237 20.0 7.5 31.0 2.0 35.5 74-75 21.265980533737718 24.5 9.5 32.5 2.0 36.0 76-77 23.122455005951807 27.5 12.5 33.0 2.0 35.5 78-79 25.34595660392856 31.0 17.5 34.5 2.0 36.5 80-81 26.95149677768938 32.5 23.5 35.0 2.0 36.5 82-83 28.003870665020674 33.0 27.5 35.0 2.0 36.0 84-85 28.026980951773833 34.0 28.0 35.0 2.0 36.0 86-87 27.752361260434046 34.0 27.0 35.0 2.0 36.0 88-89 27.32578099811332 33.0 26.5 35.0 2.0 35.0 90-91 25.570559530916896 32.5 18.0 34.5 2.0 35.0 92-93 24.051373849328016 31.5 4.5 34.0 2.0 35.0 94-95 20.272666506480785 29.0 2.0 34.0 2.0 35.0 96-97 17.530975127464238 19.5 2.0 33.0 2.0 35.0 98-99 17.822647566134908 23.5 2.0 33.0 2.0 35.0 100-101 17.03884226676098 20.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21227450648794388 1101 2 0.2938118688400486 1101 3 0.2232799332157569 1101 4 0.25249693035368637 1101 5 0.3596645802486691 1101 6 0.23962896046039361 1101 7 0.31699801365459734 1101 8 0.4246904080258105 1101 9 0.360728064543558 1101 10-11 0.3996426520781142 1101 12-13 0.34557687165834494 1101 14-15 0.5242147030229134 1101 16-17 0.49778632198586337 1101 18-19 0.47683718894079163 1101 20-21 0.4001963836756772 1101 22-23 0.3974053382569025 1101 24-25 0.40970542764721074 1101 26-27 0.4285968143788921 1101 28-29 0.4820981983309167 1101 30-31 0.2500997995979972 1101 32-33 0.34842807258425523 1101 34-35 0.30975859509349846 1101 36-37 0.24584384513899948 1101 38-39 0.15286189743640932 1101 40-41 0.28029673619798245 1101 42-43 0.4269286613125658 1101 44-45 0.3874210864438936 1101 46-47 0.31989567053231127 1101 48-49 0.15022448377801823 1101 50-51 0.1878849361899455 1101 52-53 0.04780013219983914 1101 54-55 0.09463187608759682 1101 56-57 -3.506696242370012 1101 58-59 -10.215816064394762 1101 60-61 -11.965429071048902 1101 62-63 -4.248541536087636 1101 64-65 -1.2440590557973437 1101 66-67 -2.234349237750852 1101 68-69 -4.626517798315316 1101 70-71 -4.785480919337353 1101 72-73 -3.4309625818691494 1101 74-75 -6.205470895988576 1101 76-77 -4.867670293372225 1101 78-79 1.2526458775114264 1101 80-81 1.8494373926710317 1101 82-83 0.425727725317369 1101 84-85 0.13238007993634326 1101 86-87 0.051535457291574716 1101 88-89 -0.08857210379841263 1101 90-91 1.2967897167564821 1101 92-93 0.8949751192081585 1101 94-95 -8.68368827096758 1101 96-97 -9.731800974032529 1101 98-99 -8.89092031331429 1101 100-101 -9.751800935853007 1102 1 0.2950885163451531 1102 2 0.2965040614813894 1102 3 0.3156878439501547 1102 4 0.22436028647427975 1102 5 0.2793216809810204 1102 6 0.2737078585121395 1102 7 0.28813908310497993 1102 8 0.36236565028156065 1102 9 0.2786590984590447 1102 10-11 0.2895541178100771 1102 12-13 0.2941003058531706 1102 14-15 0.3248411905171551 1102 16-17 0.38638550717193 1102 18-19 0.36364779382366663 1102 20-21 0.30724838018186773 1102 22-23 0.32506823227122794 1102 24-25 0.3090383795020415 1102 26-27 0.21998611172273996 1102 28-29 0.3401847701872853 1102 30-31 0.33559599445508326 1102 32-33 0.31683367434099097 1102 34-35 0.3733401115372388 1102 36-37 0.09536602367185054 1102 38-39 0.22772353379637167 1102 40-41 0.20576018824570497 1102 42-43 0.25413503322814535 1102 44-45 0.15563277758084126 1102 46-47 0.020719057229676707 1102 48-49 0.10146035438204137 1102 50-51 0.060164399988750006 1102 52-53 0.08046868395886264 1102 54-55 -0.17793071713998643 1102 56-57 -3.771088578838068 1102 58-59 -10.294802905397287 1102 60-61 -8.364250993431831 1102 62-63 -2.757542701269564 1102 64-65 -0.8773332389430308 1102 66-67 -2.3676949823874622 1102 68-69 -5.164681184988922 1102 70-71 -4.921675917179996 1102 72-73 -3.708261074352773 1102 74-75 -4.46524349495661 1102 76-77 -4.536222326573103 1102 78-79 1.3443567263157057 1102 80-81 1.8162444679181675 1102 82-83 0.46931927499287696 1102 84-85 0.18298323911569625 1102 86-87 0.15191031886395834 1102 88-89 0.08918028777655707 1102 90-91 1.4949152443853926 1102 92-93 0.8866362862417247 1102 94-95 -9.151458227552695 1102 96-97 -10.019177024366488 1102 98-99 -9.275630090341295 1102 100-101 -10.161443769070225 1103 1 0.23082406070481554 1103 2 0.23564500632535967 1103 3 0.21820386124851865 1103 4 0.16413464705655656 1103 5 0.09584131809918262 1103 6 0.2345982153726638 1103 7 0.26881823342803557 1103 8 0.29803660521730535 1103 9 0.2963176712067721 1103 10-11 0.27702051812811845 1103 12-13 0.33847477793349157 1103 14-15 0.4115479225453953 1103 16-17 0.3604328175628595 1103 18-19 0.3214239298681463 1103 20-21 0.2890225629015646 1103 22-23 0.4137377797726671 1103 24-25 0.3824562989106113 1103 26-27 0.43396230520347245 1103 28-29 0.30241432504225685 1103 30-31 0.3617839741084552 1103 32-33 0.26050743465465587 1103 34-35 0.25025903256519655 1103 36-37 0.4044966746313108 1103 38-39 0.4512981111179073 1103 40-41 0.3376745519845201 1103 42-43 0.2758520626559644 1103 44-45 0.23321280431647295 1103 46-47 0.22512560257250414 1103 48-49 0.2599948317771492 1103 50-51 0.24282434537158082 1103 52-53 0.22147873249144112 1103 54-55 0.2393622179545858 1103 56-57 0.7209240820950527 1103 58-59 -8.310987477189617 1103 60-61 -7.379201863288799 1103 62-63 -1.9905862199537445 1103 64-65 -0.18817590508196957 1103 66-67 -1.7340743593974217 1103 68-69 -3.536006661904924 1103 70-71 -4.0123307766070475 1103 72-73 -2.8105083293461917 1103 74-75 -3.7030031839853343 1103 76-77 -0.37155646743534376 1103 78-79 3.509588160327457 1103 80-81 2.7853687460544627 1103 82-83 1.290987831595885 1103 84-85 0.7772319068160272 1103 86-87 0.8346853828118839 1103 88-89 0.7783749253253163 1103 90-91 1.9822845408756287 1103 92-93 1.6067077441137805 1103 94-95 -7.000907090831561 1103 96-97 -8.731030686349557 1103 98-99 -8.12160944703415 1103 100-101 -9.566800578623344 1104 1 0.21644955812674027 1104 2 0.24012252057705652 1104 3 0.2062713017932083 1104 4 0.1746380726257044 1104 5 0.30460889929682367 1104 6 0.17467842028266034 1104 7 0.30338152945071784 1104 8 0.2610225942396909 1104 9 0.3592725202249838 1104 10-11 0.22405324347227662 1104 12-13 0.2827693843025756 1104 14-15 0.255475984412449 1104 16-17 0.2522397323306791 1104 18-19 0.3586412533594867 1104 20-21 0.2408005964119937 1104 22-23 0.3300872687965679 1104 24-25 0.33373935648790365 1104 26-27 0.3257668583241724 1104 28-29 0.31177096007296967 1104 30-31 0.32458769457985426 1104 32-33 0.33066745817665577 1104 34-35 0.22697336064453566 1104 36-37 0.11502499027421464 1104 38-39 0.10614566173559581 1104 40-41 0.09180203639512285 1104 42-43 0.24549102247861043 1104 44-45 0.3398744457354397 1104 46-47 0.15783932404591638 1104 48-49 0.009381689143445726 1104 50-51 0.23571460671482214 1104 52-53 0.19986240770693797 1104 54-55 0.1374024050914997 1104 56-57 0.6612363610069281 1104 58-59 -8.039146247689136 1104 60-61 -7.689025126052741 1104 62-63 -2.0983183622746964 1104 64-65 -0.24207995237158997 1104 66-67 -1.4649907746330193 1104 68-69 -2.988056875246002 1104 70-71 -3.8342606016123693 1104 72-73 -2.8518717352192056 1104 74-75 -3.515335781159525 1104 76-77 -0.14585241825640338 1104 78-79 3.813159257545337 1104 80-81 3.1329883997492765 1104 82-83 1.4997098245696456 1104 84-85 1.1458739310445019 1104 86-87 1.1397236533034736 1104 88-89 0.8104169100130711 1104 90-91 2.2442490669116033 1104 92-93 1.8477083503823337 1104 94-95 -6.248076698929715 1104 96-97 -7.781785013308806 1104 98-99 -5.8325666968353485 1104 100-101 -4.954157517631781 1105 1 0.22904300912885134 1105 2 0.27639532312161563 1105 3 0.27270219753520664 1105 4 0.24750228932494167 1105 5 0.27289350122191536 1105 6 0.2608158983171691 1105 7 0.18737840083971946 1105 8 0.2651777130064801 1105 9 0.2887673462241267 1105 10-11 0.22058618577089817 1105 12-13 0.2287748006166126 1105 14-15 0.3715007399428387 1105 16-17 0.43266078636589356 1105 18-19 0.47873041127224525 1105 20-21 0.37357092144500825 1105 22-23 0.5247221676438372 1105 24-25 0.4634141151227311 1105 26-27 0.4638308198340937 1105 28-29 0.551794845526139 1105 30-31 0.5738442327642801 1105 32-33 0.34190245528264995 1105 34-35 0.35199420624458355 1105 36-37 0.46575615933213754 1105 38-39 0.47122864618380333 1105 40-41 0.44423944021471584 1105 42-43 0.3743321654633789 1105 44-45 0.49417476488876844 1105 46-47 0.5577129794995059 1105 48-49 0.5172110923363107 1105 50-51 0.45350468558432055 1105 52-53 0.5143325252631996 1105 54-55 0.5197349204355746 1105 56-57 0.9949312264262318 1105 58-59 -8.326556755371694 1105 60-61 -7.303616962744918 1105 62-63 -1.6446876442151357 1105 64-65 0.22471505901900457 1105 66-67 -1.5081591891631412 1105 68-69 -2.90857790482071 1105 70-71 -3.4909570917592028 1105 72-73 -2.5547673672065443 1105 74-75 -3.375515288757491 1105 76-77 0.15486860406766212 1105 78-79 4.144167217835143 1105 80-81 3.638610940416431 1105 82-83 2.1083442158718775 1105 84-85 1.654609456322369 1105 86-87 1.5139577832491717 1105 88-89 1.489520013746862 1105 90-91 2.899959469543173 1105 92-93 2.67767138460162 1105 94-95 -5.925387851961213 1105 96-97 -7.753882995338042 1105 98-99 -5.479017277657729 1105 100-101 -5.007065254860127 1106 1 0.1577538484250276 1106 2 0.1779494013451064 1106 3 0.19394445366813073 1106 4 0.14638626666481258 1106 5 0.1846275277727969 1106 6 0.1737687308122844 1106 7 0.1744571455836592 1106 8 0.2165337520381314 1106 9 0.27216971195900186 1106 10-11 0.14769283422648272 1106 12-13 0.22263883565099007 1106 14-15 0.31238466779912244 1106 16-17 0.34692724039290823 1106 18-19 0.20601239098481727 1106 20-21 0.18281357512404384 1106 22-23 0.15722407884261003 1106 24-25 0.14296737057672715 1106 26-27 0.10794015508079013 1106 28-29 0.23804272727210218 1106 30-31 0.251222474830449 1106 32-33 0.2876505360113413 1106 34-35 0.3111164428692348 1106 36-37 0.29942496064253277 1106 38-39 0.31398519238029365 1106 40-41 0.31947604934761387 1106 42-43 0.34367431470337095 1106 44-45 0.3708848107701215 1106 46-47 0.3001956885498416 1106 48-49 0.22922528825525745 1106 50-51 0.19765373350750082 1106 52-53 0.2993445334204452 1106 54-55 0.21927698756635294 1106 56-57 0.8682022048467744 1106 58-59 -8.271349006074 1106 60-61 -7.52063688492149 1106 62-63 -2.005474248756137 1106 64-65 -0.10315300766977487 1106 66-67 0.9301586791509671 1106 68-69 -2.0750053448962973 1106 70-71 -2.05657258163205 1106 72-73 -0.7397613012734396 1106 74-75 -0.8601327281998543 1106 76-77 2.4302029223201025 1106 78-79 4.677262219195942 1106 80-81 3.799529347430685 1106 82-83 2.2316202894246153 1106 84-85 1.6640772603287814 1106 86-87 1.5366594697588987 1106 88-89 1.3913657096601462 1106 90-91 2.9133155932840147 1106 92-93 2.604151562440606 1106 94-95 -6.254542827431191 1106 96-97 -7.717400716543313 1106 98-99 -5.48053495608905 1106 100-101 -4.695211041898698 1107 1 0.015478101521253507 1107 2 0.07643287082136752 1107 3 0.07196825092277592 1107 4 0.024662196104763723 1107 5 0.07499173569949136 1107 6 -0.019112309166324337 1107 7 0.018410794152281085 1107 8 0.08106729154793157 1107 9 -0.0016216339778836186 1107 10-11 0.0743757715836253 1107 12-13 0.012442742563102627 1107 14-15 0.12183926490980213 1107 16-17 0.093789879383543 1107 18-19 0.09065402429759928 1107 20-21 0.15409414083572415 1107 22-23 0.12024725414938331 1107 24-25 0.08898331812145699 1107 26-27 0.10293165512662483 1107 28-29 0.08026117809769318 1107 30-31 0.12584919366167924 1107 32-33 0.056320114697072654 1107 34-35 0.04124451342502766 1107 36-37 0.0827395919231364 1107 38-39 0.18295868726032438 1107 40-41 0.1309268087823483 1107 42-43 0.0497956474497272 1107 44-45 0.012463493554058402 1107 46-47 0.019368685168259958 1107 48-49 0.007246820163970824 1107 50-51 0.028299251602341258 1107 52-53 0.006859013029178129 1107 54-55 0.02649490313240932 1107 56-57 0.5955255399626651 1107 58-59 -8.393240278791968 1107 60-61 -7.812131745816572 1107 62-63 -2.35449613862221 1107 64-65 -0.4065848513841317 1107 66-67 0.5301509671609956 1107 68-69 -2.825583151228521 1107 70-71 -2.610833586940238 1107 72-73 -1.0142075121061573 1107 74-75 -1.0903285546802408 1107 76-77 2.115985619378826 1107 78-79 4.285472029157955 1107 80-81 3.1801938265528236 1107 82-83 1.5484437624649168 1107 84-85 1.1224057719646012 1107 86-87 0.9953865841424339 1107 88-89 0.844341339690061 1107 90-91 2.166656467411496 1107 92-93 1.8031351336120913 1107 94-95 -6.825014100350339 1107 96-97 -8.287364903632099 1107 98-99 -6.013305352319318 1107 100-101 -5.347908484175925 1108 1 -0.0482783870457304 1108 2 -0.07421268473137843 1108 3 -0.07998852271152757 1108 4 -0.07270200520129322 1108 5 0.0029627980807163112 1108 6 -0.05211246901125577 1108 7 -0.03984928745076388 1108 8 -0.0556682011029892 1108 9 -0.13214457646403588 1108 10-11 -0.07798839339403685 1108 12-13 -0.08944304065179409 1108 14-15 -0.22463836564497086 1108 16-17 -0.12583022512851016 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2315 96-97 4.568291445181934 2315 98-99 6.878552135359808 2315 100-101 7.194844395464379 2316 1 -0.3726629801616568 2316 2 -0.35281454229148324 2316 3 -0.4831937192854312 2316 4 -0.3195489349353764 2316 5 -0.5183546350194632 2316 6 -0.5036624721757548 2316 7 -0.324275725187519 2316 8 -0.36631770251881335 2316 9 -0.6403112511396145 2316 10-11 -0.5204110551233114 2316 12-13 -0.46842448409635296 2316 14-15 -0.5165385116806718 2316 16-17 -0.5758300273474077 2316 18-19 -0.6024794343268027 2316 20-21 -0.5087286966392739 2316 22-23 -0.7517867957348514 2316 24-25 -0.5793807550209635 2316 26-27 -0.5376210543269124 2316 28-29 -0.5243762883821361 2316 30-31 -0.519621590484526 2316 32-33 -0.5121403529619784 2316 34-35 -0.44060178042056464 2316 36-37 -0.36931717740260694 2316 38-39 -0.5184328803893123 2316 40-41 -0.5850566162949491 2316 42-43 -0.5462323366839641 2316 44-45 -0.5588768457454236 2316 46-47 -0.5072206186815649 2316 48-49 -0.5158630072391688 2316 50-51 -0.48155131386088357 2316 52-53 -0.40504808768428546 2316 54-55 -0.3201854271027784 2316 56-57 0.43625886652391443 2316 58-59 3.6555734526262462 2316 60-61 3.602090472970886 2316 62-63 0.9811260938530921 2316 64-65 0.39097421682301814 2316 66-67 -1.6236651655745185 2316 68-69 -2.8667682880738816 2316 70-71 -3.06175416126192 2316 72-73 -0.5241507045852458 2316 74-75 5.8475913553872125 2316 76-77 6.134641436039605 2316 78-79 5.146254627495484 2316 80-81 3.4997892816836362 2316 82-83 1.7292698289927344 2316 84-85 1.3365400378150625 2316 86-87 1.152407433283603 2316 88-89 1.2336919600309848 2316 90-91 2.750545222253983 2316 92-93 4.23328693268288 2316 94-95 6.918128249427255 2316 96-97 4.060873514814176 2316 98-99 6.3931608307944465 2316 100-101 6.517599763938449 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 46.0 5 116.0 6 265.0 7 721.0 8 1626.0 9 2866.0 10 4547.0 11 7010.0 12 10074.0 13 13750.0 14 17270.0 15 22842.0 16 30420.0 17 39930.0 18 43674.0 19 44003.0 20 46744.0 21 47953.0 22 48446.0 23 49846.0 24 52616.0 25 57099.0 26 70855.0 27 99708.0 28 140719.0 29 185161.0 30 225524.0 31 254376.0 32 270490.0 33 271211.0 34 278496.0 35 297972.0 36 300858.0 37 230602.0 38 89650.0 39 5360.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.346789254065165 19.732639140089784 14.042545495121558 23.878026110723496 2 15.549739198828364 21.29521184558707 39.21259103979314 23.94245791579143 3 18.781036700500877 25.553078868272756 30.422655385395252 25.24322904583111 4 12.823870720186731 17.29992190938301 37.44803664006845 32.428170730361806 5 13.188299977521895 38.236085117501 34.78132606101717 13.794288843959936 6 31.43138139481856 37.14333612432713 17.598707398013286 13.826575082841028 7 27.64104428118016 33.05467323919144 21.775329494593112 17.528952985035286 8 25.829966413035383 35.57607053660992 20.16725750588515 18.426705544469552 9 26.25910393162351 16.344844241705783 20.358297403885405 37.0377544227853 10-11 24.540221571398973 26.64883679306232 28.222984875919284 20.587956759619427 12-13 25.588744408307274 24.14541649759292 28.34891672254784 21.91692237155197 14-15 22.52370619640438 25.344559680770857 26.432531582721907 25.699202540102856 16-17 22.038168480531972 28.61040821202035 27.04459609323126 22.30682721421642 18-19 22.007049942481505 27.53326947327095 28.896489234073076 21.56319135017447 20-21 22.72268391443622 26.7291034044812 28.689766328846083 21.858446352236495 22-23 22.532590879947627 26.693153524681907 28.55693872522278 22.217316870147684 24-25 22.439959747136996 26.794848160678004 28.60828040069589 22.15691169148911 26-27 22.30689027737212 26.910701090725876 28.658501265602432 22.123907366299576 28-29 22.195680943177756 27.118276030527987 28.646836101778757 22.0392069245155 30-31 22.346173945158835 26.815240161890706 28.600773287113874 22.237812605836584 32-33 22.233388931900645 26.92225763284962 28.55498281470965 22.289370620540083 34-35 22.593468058615077 26.878191744085818 28.440111100469306 22.088229096829807 36-37 22.51682564454595 26.666997934483817 28.503187839281125 22.312988581689112 38-39 22.292109736296 27.068827179994592 28.564273441463286 22.07478964224613 40-41 22.448111996559767 26.897857000229735 28.502568673562063 22.15146232964843 42-43 22.203211403710032 27.011820380279083 28.2560491415685 22.528919074442385 44-45 22.416350719623072 26.884976871449428 28.219132280585512 22.479540128341984 46-47 22.526249729117424 27.063396721005752 27.987472457396095 22.42288109248073 48-49 22.51329639184032 27.095625141275605 27.934553013966383 22.456525452917692 50-51 22.24237329512416 27.232608364963042 28.025886265552124 22.499132074360677 52-53 22.619187439837816 27.373422136985326 27.5928809065504 22.41450951662646 54-55 22.342839209789492 27.041975314698725 27.75369338952916 22.861492085982622 56-57 22.304425303651083 27.26933842100671 27.86508628614513 22.561149989197077 58-59 21.535111381615984 26.87998143228215 29.20551401961462 22.379393166487244 60-61 21.756921050612476 26.99845149041165 28.928729965359572 22.315897493616298 62-63 22.338863094506536 27.160182041695258 27.978198885670423 22.52275597812778 64-65 22.255595175721627 27.24235726587856 27.819915388325043 22.68213217007477 66-67 21.978717648622744 26.964012864478313 28.40082412483217 22.656445362066773 68-69 21.17413030581721 26.49612824046028 29.893436370998373 22.436305082724136 70-71 22.013528063639768 26.527406818824435 28.923014497447248 22.536050620088552 72-73 21.43753460105568 26.604755612782743 29.216616495786084 22.741093290375492 74-75 21.560795229467626 26.941428229044167 28.75962246531318 22.73815407617503 76-77 20.5920522239551 27.129170735984896 29.087584490779694 23.191192549280306 78-79 21.8006865183238 27.016385569799112 28.148823314004456 23.03410459787263 80-81 21.97337656723401 27.112834103600708 27.681781362615958 23.23200796654932 82-83 22.14663273746787 27.14082037517243 27.533894209183135 23.178652678176565 84-85 22.05365209897157 27.09828198536488 27.434186088421235 23.41387982724231 86-87 21.952813968242292 27.077659577728536 27.551466058605566 23.418060395423606 88-89 22.02885492089032 26.93872734934682 27.34497950427154 23.687438225491324 90-91 21.75651661044927 26.936763347877385 27.771260338768112 23.535459702905236 92-93 21.737175276787845 26.99611365519407 27.720257380651436 23.54645368736665 94-95 20.568979818896977 26.84824306801527 28.549669052434606 24.033108060653145 96-97 20.15455960655515 26.92301639597649 28.6183948618077 24.30402913566066 98-99 20.976613483924805 26.67809561234283 28.042320616780593 24.30297028695177 100-101 20.169988112266804 26.745981918440346 28.629141250742407 24.454888718550443 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 532.0 1 429.5 2 377.0 3 448.0 4 478.5 5 537.5 6 721.0 7 1077.0 8 1506.5 9 1857.5 10 2030.0 11 2138.0 12 2338.0 13 2603.0 14 2823.0 15 3085.5 16 3405.0 17 3662.5 18 3993.0 19 4416.5 20 4946.0 21 5767.0 22 6822.5 23 8110.5 24 9934.5 25 12732.0 26 16469.0 27 20988.0 28 25707.5 29 31049.5 30 37321.0 31 44112.0 32 51541.0 33 59105.0 34 67165.5 35 74979.0 36 82384.5 37 89595.0 38 94521.0 39 97736.5 40 101684.5 41 105906.0 42 110211.5 43 115355.5 44 119936.0 45 123126.0 46 126291.0 47 130423.0 48 134378.0 49 134079.0 50 129225.0 51 123765.5 52 118548.0 53 113556.0 54 107594.0 55 99136.5 56 90113.5 57 80633.0 58 70988.5 59 61197.0 60 50056.0 61 39036.0 62 29481.0 63 21747.0 64 15485.5 65 10736.0 66 7327.5 67 4901.0 68 3151.0 69 1964.5 70 1278.0 71 850.0 72 566.5 73 389.5 74 259.0 75 152.5 76 86.5 77 38.5 78 15.5 79 8.0 80 3.5 81 3.0 82 4.0 83 1.5 84 0.0 85 1.0 86 2.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02353751720874468 2 0.013147910003322223 3 1.5323904432776485E-4 4 0.0 5 0.05899703206618946 6 0.0 7 0.0 8 9.19434265966589E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 4.903649418488475E-4 16-17 1.5323904432776483E-5 18-19 3.830976108194121E-4 20-21 0.0 22-23 0.0 24-25 7.661952216388242E-5 26-27 9.19434265966589E-5 28-29 0.0010420255014288009 30-31 0.005547253404665087 32-33 0.006190857390841699 34-35 0.0019461358629626134 36-37 0.08195224090648863 38-39 0.07594527036884025 40-41 0.08069568074300096 42-43 0.08409758752707734 44-45 0.08691718594270821 46-47 0.08273376003256024 48-49 0.07370798032165488 50-51 0.068283318152452 52-53 0.05820018903568508 54-55 0.04727424517511545 56-57 0.06695013846680045 58-59 0.7001185457246919 60-61 0.33833648597127197 62-63 0.003279315548614167 64-65 0.0026816832757358845 66-67 1.0939735374559132 68-69 2.359452213323461 70-71 2.338887533574675 72-73 1.792712931781655 74-75 1.2800976806964162 76-77 0.7419528048261718 78-79 0.5725010696085294 80-81 0.03794198737555457 82-83 9.19434265966589E-5 84-85 0.0012259123546221188 86-87 0.0014097992078154363 88-89 3.064780886555297E-5 90-91 0.33458212938524173 92-93 0.05253034439555779 94-95 0.9437379783969725 96-97 1.6515338002424853 98-99 0.6379494654409177 100-101 0.5625405317272246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3262876.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.956643163292526 #Duplication Level Percentage of deduplicated Percentage of total 1 68.45283543267071 30.774096960621637 2 14.934030094796539 13.427677239232793 3 6.19273738043946 8.352140538491993 4 3.1129945427248096 5.597991393062251 5 1.8486528710647339 4.155461372862673 6 1.230224973419544 3.3184071084356086 7 0.8315082824654266 2.616727479948431 8 0.5838857648230763 2.0999635181821747 9 0.44351108280416524 1.7944892539733108 >10 2.1851355378633737 17.13462126040945 >50 0.12263993218132063 3.788037747079396 >100 0.057674598497281786 4.611433918755795 >500 0.003075865267040297 0.8933539867500568 >1k 8.202307369765383E-4 0.583421119195668 >5k 2.734102456588461E-4 0.8521771029987498 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9858 0.30212609979662114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7715 0.23644784539774114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5117 0.15682483796503452 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5042 0.15452625230011804 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.064780886555297E-5 2 0.0 0.0 0.0 0.0 3.064780886555297E-5 3 0.0 0.0 0.0 0.0 3.064780886555297E-5 4 0.0 0.0 0.0 0.0 3.064780886555297E-5 5 0.0 0.0 0.0 0.0 3.064780886555297E-5 6 6.129561773110593E-5 0.0 0.0 0.0 3.064780886555297E-5 7 6.129561773110593E-5 0.0 0.0 0.0 3.064780886555297E-5 8 6.129561773110593E-5 0.0 0.0 0.0 3.064780886555297E-5 9 6.129561773110593E-5 0.0 0.0 0.0 3.064780886555297E-5 10-11 6.129561773110593E-5 0.0 0.0 0.0 3.064780886555297E-5 12-13 6.129561773110593E-5 0.0 0.0 6.129561773110593E-5 3.064780886555297E-5 14-15 7.661952216388241E-5 0.0 0.0 6.129561773110593E-5 3.064780886555297E-5 16-17 9.19434265966589E-5 0.0 0.0 2.4518247092442374E-4 3.064780886555297E-5 18-19 1.2259123546221187E-4 0.0 0.0 3.371258975210826E-4 3.064780886555297E-5 20-21 1.5323904432776482E-4 0.0 0.0 5.056888462816239E-4 3.064780886555297E-5 22-23 1.9921075762609428E-4 0.0 0.0 9.80729883697695E-4 6.129561773110593E-5 24-25 2.4518247092442374E-4 0.0 0.0 0.0014251231122482128 1.2259123546221187E-4 26-27 2.4518247092442374E-4 0.0 0.0 0.0019461358629626131 1.2259123546221187E-4 28-29 3.9842151525218856E-4 0.0 0.0 0.004260045432311862 1.2259123546221187E-4 30-31 4.903649418488475E-4 0.0 0.0 0.01084932433840575 1.2259123546221187E-4 32-33 4.903649418488475E-4 0.0 0.0 0.026678917617463854 1.5323904432776482E-4 34-35 5.516605595799534E-4 0.0 0.0 0.04922038103807806 1.9921075762609428E-4 36-37 6.436039861766122E-4 0.0 0.0 0.08351527915863183 2.1453466205887076E-4 38-39 7.048996039077183E-4 0.0 0.0 0.13477373948626917 2.1453466205887076E-4 40-41 7.048996039077183E-4 0.0 0.0 0.19387803888348806 2.1453466205887076E-4 42-43 7.508713172060476E-4 0.0 0.0 0.25498976976140064 2.2985856649164726E-4 44-45 7.968430305043771E-4 0.0 0.0 0.3281614134279084 2.4518247092442374E-4 46-47 8.88786457101036E-4 0.0 0.0 0.41207511410179243 2.605063753572002E-4 48-49 8.88786457101036E-4 0.0 0.0 0.4993294259420217 2.758302797899767E-4 50-51 8.88786457101036E-4 0.0 0.0 0.5911962330165167 2.758302797899767E-4 52-53 8.88786457101036E-4 0.0 0.0 0.6826646185757596 2.9115418422275317E-4 54-55 8.88786457101036E-4 0.0 0.0 0.7852734826576309 3.0647808865552964E-4 56-57 8.88786457101036E-4 0.0 0.0 0.8830706407476103 3.0647808865552964E-4 58-59 8.88786457101036E-4 0.0 0.0 0.9575907880042025 3.0647808865552964E-4 60-61 8.88786457101036E-4 0.0 0.0 1.0184266886023252 3.0647808865552964E-4 62-63 8.88786457101036E-4 0.0 0.0 1.0748186569149425 3.0647808865552964E-4 64-65 8.88786457101036E-4 0.0 0.0 1.1389338730616791 3.0647808865552964E-4 66-67 8.88786457101036E-4 0.0 0.0 1.19468223738812 3.0647808865552964E-4 68-69 9.19434265966589E-4 0.0 0.0 1.264145496181896 3.0647808865552964E-4 70-71 9.654059792649184E-4 0.0 0.0 1.3659881650421286 3.0647808865552964E-4 72-73 9.80729883697695E-4 0.0 0.0 1.5004247786308764 3.677737063866356E-4 74-75 9.80729883697695E-4 0.0 0.0 1.6842043644931648 3.677737063866356E-4 76-77 9.80729883697695E-4 0.0 0.0 1.9201311971401918 3.830976108194121E-4 78-79 0.0010113776925632478 0.0 0.0 2.217782716842442 3.9842151525218856E-4 80-81 0.0011646167368910127 0.0 0.0 2.5705236729805243 3.9842151525218856E-4 82-83 0.0011646167368910127 0.0 0.0 2.920981367358122 3.9842151525218856E-4 84-85 0.0011646167368910127 0.0 0.0 3.2367610660043473 3.9842151525218856E-4 86-87 0.0012105884501893423 1.5323904432776483E-5 0.0 3.529432316765945 4.1374541968496503E-4 88-89 0.0012259123546221186 3.064780886555297E-5 3.064780886555297E-5 3.8147940651131087 4.290693241177415E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11175 0.0 26.131111 1 GTATCAA 16110 0.0 26.003523 1 TACATGG 11045 0.0 25.407743 2 GGTATCA 11545 0.0 23.618301 1 ACATGGG 11375 0.0 23.5897 3 TCAACGC 18960 0.0 21.987482 4 CAACGCA 19035 0.0 21.876074 5 ATCAACG 19060 0.0 21.872124 3 AACGCAG 19155 0.0 21.799181 6 TATCAAC 19540 0.0 21.434002 2 GAGTACT 8810 0.0 20.729609 12-13 CATGGGA 6960 0.0 19.98823 4 AGAGTAC 14430 0.0 19.579947 10-11 CATGGGG 5285 0.0 19.452394 4 ACGCAGA 21190 0.0 19.416544 7 CGCAGAG 21045 0.0 19.393562 8 AGTACTT 9405 0.0 18.942106 12-13 GTACTTT 9635 0.0 18.685595 14-15 GCAGAGT 21745 0.0 17.858973 9 CGCATAG 410 0.0 17.808317 66-67 >>END_MODULE