FastQCFastQC Report
Fri 27 May 2016
SRR939659_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939659_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600157
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT127000.48843204468037893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79620.3062122787200927No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67170.2583305546549689No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT56580.2176022447875263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54660.21021807529314576No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC46950.18056601966727395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38530.14818335969712598No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38340.14745263459091124No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33030.1270307908330151No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28790.1107240831995914No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28100.10807039728754841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG91750.026.0170122
GTACATG94800.025.3792291
ACATGGG92200.024.7057173
GAGTACT74150.019.77581812-13
AGTACTT79250.019.31157112-13
CATGGGA55550.019.2221664
GTATAGG7950.019.1012421
GTACTTT77350.018.8660214-15
CATGGGG45050.018.8565444
GTATAAT16400.017.9401741
AGAGTAC138700.017.6035510-11
AGTACAT69800.017.2692622
ATGGGAG26800.016.1139535
CATGGGC20400.016.0517644
ATGGGAT25550.015.9736085
ACTTTTT99450.015.62885416-17
TACTTTT96850.015.60649314-15
GTATATG12300.015.4324061
GAGTACA97050.014.9625171
GTGTAGC15550.014.9535551
Produced by FastQC (version 0.11.5)