FastQCFastQC Report
Fri 27 May 2016
SRR939659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2600157
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT139670.5371598715000672No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86310.33194149430207487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74480.2864442416361781No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64610.2484849953291282No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT62120.23890865051610344No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54640.21014115686091264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47130.1812582855573721No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41720.1604518496383103No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39440.15168314836373342No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34570.13295351011496614No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33580.12914604771942617No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28920.11122405300910676No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27770.10680124315570175No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27480.10568592588832135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG89400.025.8416272
GTACATG95250.025.100561
ACATGGG90400.024.460873
CATGGGA53650.020.7836154
CATGGGG43050.020.2181344
GAGTACT78900.019.59113712-13
GTACTTT81150.018.75828214-15
AGTACTT80000.018.67574312-13
TACGCGG1202.4516303E-618.25216364-65
ATGGGAT25600.017.6430535
AGAGTAC140650.017.16975610-11
GGACCGA4801.8189894E-1216.6394946
AGTACAT69300.016.2270872
GCTTACG5700.016.01579960-61
CGCGTAG1551.6031845E-615.70047166-67
TACTTTT95850.015.39125714-15
ACTTTTT99300.015.35316316-17
GAGTACA93950.015.2787471
ATGGGGA25950.015.2294575
GTATCAA257900.015.12822151
Produced by FastQC (version 0.11.5)