##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939659_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2600157 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.566159658820602 31.0 31.0 34.0 30.0 34.0 2 31.778109552615476 31.0 31.0 34.0 30.0 34.0 3 31.830203330029686 31.0 31.0 34.0 30.0 34.0 4 35.60064757628097 37.0 35.0 37.0 33.0 37.0 5 35.34198857992037 37.0 35.0 37.0 33.0 37.0 6 35.365929057360766 37.0 35.0 37.0 33.0 37.0 7 35.298575432175824 37.0 35.0 37.0 32.0 37.0 8 35.270600582964796 37.0 35.0 37.0 32.0 37.0 9 36.88117948262355 39.0 37.0 39.0 33.0 39.0 10-11 36.76241684636736 39.0 36.0 39.0 32.0 39.0 12-13 36.7159763814262 39.0 35.0 39.0 32.0 39.0 14-15 37.77112766652168 40.0 37.0 41.0 32.0 41.0 16-17 37.74842942176184 39.0 37.0 41.0 32.0 41.0 18-19 37.74271995883325 39.5 37.0 41.0 32.0 41.0 20-21 37.677604275434135 39.5 37.0 41.0 32.0 41.0 22-23 37.5423439815365 39.0 36.0 41.0 32.0 41.0 24-25 37.414503816500314 39.0 36.0 41.0 32.0 41.0 26-27 37.15837543656018 39.0 36.0 40.5 31.0 41.0 28-29 36.97923740758731 39.0 36.0 40.0 31.0 41.0 30-31 36.76474612879145 39.0 35.5 40.0 30.0 41.0 32-33 36.59871500067111 38.5 35.0 40.0 30.0 41.0 34-35 36.57140818804403 38.0 35.0 40.0 30.0 41.0 36-37 36.47176747404099 38.5 35.0 40.0 30.0 41.0 38-39 36.493956711075526 39.0 35.0 40.0 30.0 41.0 40-41 36.42192586832257 39.0 35.0 40.0 30.0 41.0 42-43 36.25010335914331 39.0 35.0 40.0 30.0 41.0 44-45 36.04873648014332 38.0 35.0 40.0 29.5 41.0 46-47 35.77515146200787 38.0 35.0 40.0 28.5 41.0 48-49 35.492936964960194 38.0 34.5 40.0 27.0 41.0 50-51 35.25930568807961 38.0 34.0 40.0 27.0 41.0 52-53 35.042555507225146 38.0 34.0 40.0 26.5 41.0 54-55 34.774357279195065 38.0 34.0 40.0 26.0 41.0 56-57 32.79442087535483 37.0 30.5 39.5 16.5 41.0 58-59 27.838812040965216 33.0 18.0 38.5 7.5 40.0 60-61 28.587497985698555 33.5 21.0 38.0 9.5 40.0 62-63 31.63669251510582 35.0 30.0 38.5 15.5 40.0 64-65 31.880603940454364 36.0 31.0 39.0 10.5 40.0 66-67 25.17135638347992 28.0 18.0 35.5 2.0 39.0 68-69 17.617016780140585 13.0 7.5 28.5 2.0 35.5 70-71 16.47890723521695 13.0 7.5 27.0 2.0 34.0 72-73 17.653039989508326 15.0 7.5 29.0 2.0 34.0 74-75 18.701571289733657 17.5 9.0 30.0 2.0 34.5 76-77 19.888538653627457 22.0 8.0 30.0 2.0 34.5 78-79 23.04162152516175 27.5 10.5 33.5 2.0 36.0 80-81 26.65614403284109 31.5 24.0 35.0 2.0 36.0 82-83 27.927866471140014 33.5 27.5 35.0 2.0 36.0 84-85 27.962332659143275 34.0 28.0 35.0 2.0 36.0 86-87 27.740234724287802 34.0 27.5 35.0 2.0 36.0 88-89 27.32553207364017 33.5 26.5 35.0 2.0 35.5 90-91 26.036492604100445 33.0 22.5 35.0 2.0 35.0 92-93 23.645470446592263 31.5 3.5 34.5 2.0 35.0 94-95 16.237187600594886 8.5 2.0 32.0 2.0 35.0 96-97 13.630612689926032 2.0 2.0 30.0 2.0 34.0 98-99 13.315913423689416 2.0 2.0 30.5 2.0 34.0 100-101 13.025484999559641 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42093710130960105 1101 2 0.3879068432003123 1101 3 0.43741874522419977 1101 4 0.33575755285076525 1101 5 0.39050677712820203 1101 6 0.40970953780144015 1101 7 0.46509565305824196 1101 8 0.5045824527344323 1101 9 0.6203140675325116 1101 10-11 0.42417146246182824 1101 12-13 0.5217545812602538 1101 14-15 0.6821701835138327 1101 16-17 0.6460066085639866 1101 18-19 0.6269460447465391 1101 20-21 0.5415620315121146 1101 22-23 0.6981226706444019 1101 24-25 0.6591576573856699 1101 26-27 0.6518982740139663 1101 28-29 0.6992021633683692 1101 30-31 0.6347077151579796 1101 32-33 0.6861803065133287 1101 34-35 0.6590538606701699 1101 36-37 0.5002075312650831 1101 38-39 0.5629503516531074 1101 40-41 0.4497316467239969 1101 42-43 0.5431323556029639 1101 44-45 0.5234756179011342 1101 46-47 0.5076873450537533 1101 48-49 0.5759366953591396 1101 50-51 0.37369266411952395 1101 52-53 0.4615036971184523 1101 54-55 0.4034355842332573 1101 56-57 -1.8206690982117664 1101 58-59 -9.501181317914519 1101 60-61 -9.92563919398765 1101 62-63 -3.6782380617612134 1101 64-65 -4.587713453451613 1101 66-67 -9.465156522941555 1101 68-69 -4.326888011862831 1101 70-71 -4.56765717148221 1101 72-73 -4.779933992465738 1101 74-75 -6.732757370784579 1101 76-77 -7.846981026057264 1101 78-79 -11.410494418824914 1101 80-81 -8.06720333702783 1101 82-83 -4.192102058501639 1101 84-85 -3.3334286958885393 1101 86-87 -3.062571093837885 1101 88-89 -3.100672802870097 1101 90-91 -9.717982673939474 1101 92-93 -16.99168330756013 1101 94-95 -11.450747879774983 1101 96-97 -9.59645775659123 1101 98-99 -9.527894753244723 1101 100-101 -9.435729239767335 1102 1 0.29913811865387885 1102 2 0.30119058164170553 1102 3 0.3127649213098991 1102 4 0.2107190990263348 1102 5 0.3789957462430422 1102 6 0.25635843595958363 1102 7 0.33147104231728264 1102 8 0.37377675023851253 1102 9 0.34147278255327507 1102 10-11 0.3461330236705962 1102 12-13 0.3010926951510413 1102 14-15 0.49594220760948104 1102 16-17 0.48579963440605667 1102 18-19 0.40183928153007287 1102 20-21 0.5297138536275625 1102 22-23 0.5736782115334194 1102 24-25 0.5180428466408245 1102 26-27 0.43145638594677393 1102 28-29 0.48259869113927323 1102 30-31 0.30243312240185816 1102 32-33 0.5027499723766269 1102 34-35 0.33185718938722175 1102 36-37 0.39560707887576996 1102 38-39 0.42264055041034254 1102 40-41 0.3145400999287702 1102 42-43 0.31479400448095163 1102 44-45 0.5558054301658117 1102 46-47 0.44423569310678346 1102 48-49 0.4227216256399302 1102 50-51 0.364173165509726 1102 52-53 0.1625221413876332 1102 54-55 0.22553123424863486 1102 56-57 -2.246096361909121 1102 58-59 -9.947628477868129 1102 60-61 -10.182218327100028 1102 62-63 -4.053836868931828 1102 64-65 -4.906368700753241 1102 66-67 -6.812558731061905 1102 68-69 -4.251434000070201 1102 70-71 -4.213836887149407 1102 72-73 -4.496656080305822 1102 74-75 -5.459940365522593 1102 76-77 -7.642795893304946 1102 78-79 -10.428603190990177 1102 80-81 -5.975639235281669 1102 82-83 -3.183565767807959 1102 84-85 -2.654317656643453 1102 86-87 -2.5768229336631734 1102 88-89 -2.673983300406668 1102 90-91 -8.98033198903057 1102 92-93 -13.769803182293245 1102 94-95 -10.828462701446405 1102 96-97 -9.471862415489765 1102 98-99 -9.519094464900622 1102 100-101 -9.487028016541554 1103 1 0.3003067193146194 1103 2 0.25339151292038764 1103 3 0.3092356072424707 1103 4 0.2338559913890137 1103 5 0.3693702987630374 1103 6 0.3302964400937043 1103 7 0.32476859726052254 1103 8 0.32972710872277844 1103 9 0.4688637615164666 1103 10-11 0.5228561093366295 1103 12-13 0.49277271868313477 1103 14-15 0.663136145293457 1103 16-17 0.5677079852984193 1103 18-19 0.4993453996748016 1103 20-21 0.48504164211085055 1103 22-23 0.4345854678631724 1103 24-25 0.5830099897523837 1103 26-27 0.6318176216219058 1103 28-29 0.4882468442899963 1103 30-31 0.5403414072602715 1103 32-33 0.4842419592578935 1103 34-35 0.38201046817250983 1103 36-37 0.32523565796542897 1103 38-39 0.2440450022986127 1103 40-41 0.4327438566597337 1103 42-43 0.4858246642400701 1103 44-45 0.3395210099738293 1103 46-47 0.3830682530425946 1103 48-49 0.3971287208868901 1103 50-51 0.32462308676925034 1103 52-53 0.36360174630456044 1103 54-55 0.3060751338130103 1103 56-57 -2.7925084677315155 1103 58-59 -10.628044711812375 1103 60-61 -10.521475705392472 1103 62-63 -4.169703174212746 1103 64-65 -5.519321508496425 1103 66-67 -6.604278675113147 1103 68-69 -4.128224920603355 1103 70-71 -4.197140555234803 1103 72-73 -4.376697671683791 1103 74-75 -5.273597585333576 1103 76-77 -7.0755568324276545 1103 78-79 -9.365396202598143 1103 80-81 -5.049273333208003 1103 82-83 -2.6971690098743792 1103 84-85 -2.238867865450782 1103 86-87 -2.047655191443674 1103 88-89 -1.9869999409599117 1103 90-91 -4.938570773765925 1103 92-93 -9.895480780578763 1103 94-95 -8.485335584108093 1103 96-97 -7.961417046570141 1103 98-99 -8.243169639540387 1103 100-101 -8.396429699006342 1104 1 0.23871538219912836 1104 2 0.2724814471294863 1104 3 0.1609034423039759 1104 4 0.17102937507711147 1104 5 0.1605866061136041 1104 6 0.12127587689964514 1104 7 0.1945097797649069 1104 8 0.2668871710445657 1104 9 0.317673330919952 1104 10-11 0.3143232330371717 1104 12-13 0.3124195970152073 1104 14-15 0.459181309165686 1104 16-17 0.3519169070614723 1104 18-19 0.37870072325355864 1104 20-21 0.38875251684171275 1104 22-23 0.3753330712661125 1104 24-25 0.4564708168871192 1104 26-27 0.3951688916199174 1104 28-29 0.3919389858494924 1104 30-31 0.4800416876195257 1104 32-33 0.41755728099386147 1104 34-35 0.41958185543184356 1104 36-37 0.2718657547062975 1104 38-39 0.32113165556620515 1104 40-41 0.3004465925908377 1104 42-43 0.36331270692617323 1104 44-45 0.4289966726701735 1104 46-47 0.47294085082558723 1104 48-49 0.4607030682648272 1104 50-51 0.4744668798728924 1104 52-53 0.4692421931919952 1104 54-55 0.2705965887060344 1104 56-57 -2.677223767931281 1104 58-59 -10.46349406092487 1104 60-61 -10.690650951851627 1104 62-63 -4.392601756430832 1104 64-65 -5.500122019925879 1104 66-67 -6.847513502493218 1104 68-69 -4.395640317438533 1104 70-71 -4.3830883038406405 1104 72-73 -4.551628372826155 1104 74-75 -5.265116592980597 1104 76-77 -6.9363836618814165 1104 78-79 -9.592196210590963 1104 80-81 -5.368884618514482 1104 82-83 -3.0595565900315407 1104 84-85 -2.6396985497749164 1104 86-87 -2.535377124018691 1104 88-89 -2.3867429912308644 1104 90-91 -4.939911860689506 1104 92-93 -7.02047016511499 1104 94-95 -7.850120732671856 1104 96-97 -7.945048140193258 1104 98-99 -8.310299057169106 1104 100-101 -8.353713142157687 1105 1 0.2281928388265584 1105 2 0.23476718194974922 1105 3 0.24413665313174704 1105 4 0.17097087549120005 1105 5 0.18752803326393064 1105 6 0.19075987433792108 1105 7 0.22174732624770144 1105 8 0.20857798736172128 1105 9 0.3291867458499951 1105 10-11 0.33192875305809366 1105 12-13 0.29666287283602344 1105 14-15 0.47336084272341594 1105 16-17 0.40165736389867135 1105 18-19 0.30845490799358544 1105 20-21 0.3966864535379173 1105 22-23 0.5163706282788354 1105 24-25 0.4547874913026604 1105 26-27 0.48726200401795694 1105 28-29 0.5823638432706773 1105 30-31 0.5250259191954285 1105 32-33 0.5348535471324354 1105 34-35 0.5045161506469427 1105 36-37 0.47548832763508386 1105 38-39 0.392241384353774 1105 40-41 0.4515705924916702 1105 42-43 0.384400345155818 1105 44-45 0.34595100872912354 1105 46-47 0.4330490778452045 1105 48-49 0.4417636184005005 1105 50-51 0.4500413527707323 1105 52-53 0.4786143839931256 1105 54-55 0.5031524300428956 1105 56-57 1.6167383226656398 1105 58-59 -8.652918923638875 1105 60-61 -9.507577612035053 1105 62-63 -3.3797521831017363 1105 64-65 -4.61282007932747 1105 66-67 -6.220010242534148 1105 68-69 -3.8063393763473368 1105 70-71 -3.911824971854653 1105 72-73 -2.166283661230505 1105 74-75 -4.251133729459294 1105 76-77 -6.398268395968763 1105 78-79 -9.085185174504797 1105 80-81 -4.635475797501208 1105 82-83 -2.0194716987753054 1105 84-85 -1.462404551191721 1105 86-87 -1.4350310432541598 1105 88-89 -1.5780466656455694 1105 90-91 -4.181959956263345 1105 92-93 -6.4058959404225355 1105 94-95 -8.008444360181036 1105 96-97 -8.287887958019976 1105 98-99 -8.622140222993425 1105 100-101 -8.473188378586551 1106 1 0.1757561956808793 1106 2 0.20131251663970673 1106 3 0.08865053593385497 1106 4 0.04052055966923973 1106 5 -0.025404420195947353 1106 6 0.1474041331369591 1106 7 0.16158551769249385 1106 8 0.15056176184820913 1106 9 0.1609542723606765 1106 10-11 0.13279653441925632 1106 12-13 0.1750356757213325 1106 14-15 0.15258942930142183 1106 16-17 0.243911602491643 1106 18-19 0.26940441347301913 1106 20-21 0.1477095047249648 1106 22-23 0.19143469082935383 1106 24-25 0.2301667259380551 1106 26-27 0.30501875328968 1106 28-29 0.3780409666830238 1106 30-31 0.2792035240142283 1106 32-33 0.25014420898047973 1106 34-35 0.20431448983636358 1106 36-37 0.34150786450508264 1106 38-39 0.35205969299578754 1106 40-41 0.20369601278499516 1106 42-43 0.17268273894395492 1106 44-45 0.15184931249209654 1106 46-47 0.3154660114877714 1106 48-49 0.23846904197984742 1106 50-51 0.28031683590183576 1106 52-53 0.2710903794913335 1106 54-55 0.031127576872698626 1106 56-57 1.6186232896870507 1106 58-59 -8.402532748685541 1106 60-61 -10.10023027320123 1106 62-63 -3.7918057740205704 1106 64-65 -5.433406612011286 1106 66-67 -6.983316989922905 1106 68-69 -4.362902134318038 1106 70-71 -4.040795741868347 1106 72-73 -4.370385164171768 1106 74-75 -4.863772090071215 1106 76-77 -6.655891664513577 1106 78-79 -9.425897962652968 1106 80-81 -5.0007039048390105 1106 82-83 -2.5604547344053827 1106 84-85 -2.1433877134638557 1106 86-87 -1.9488639383948687 1106 88-89 -1.9718063116876863 1106 90-91 -4.370472738612637 1106 92-93 -6.272908450991316 1106 94-95 -7.441061871511332 1106 96-97 -7.159789601800535 1106 98-99 -6.5741389679039965 1106 100-101 -7.1089539948018 1107 1 0.04840555064784269 1107 2 -0.011121150089497434 1107 3 0.0894891291063189 1107 4 0.09341155130567103 1107 5 0.011756792860829535 1107 6 0.028264928938547484 1107 7 0.10271209698086636 1107 8 0.11903837051001886 1107 9 0.20121273921922977 1107 10-11 0.17977857109246997 1107 12-13 0.07744463829926929 1107 14-15 0.08578760915244033 1107 16-17 -2.495953356671521E-4 1107 18-19 0.0954186073429284 1107 20-21 0.1366326545301817 1107 22-23 0.18339693080632458 1107 24-25 0.3481273470543442 1107 26-27 0.24795286053952736 1107 28-29 0.18702690728613192 1107 30-31 0.30605446407782466 1107 32-33 0.13882209944556934 1107 34-35 0.1349787444135444 1107 36-37 0.09847202760821716 1107 38-39 0.0658523472695478 1107 40-41 0.0427483376938298 1107 42-43 -0.052715908743543594 1107 44-45 -0.07818128254938728 1107 46-47 -0.03525508791176435 1107 48-49 -0.2437822210133973 1107 50-51 -0.1517480442502972 1107 52-53 0.02453669608099318 1107 54-55 -0.07302525770507629 1107 56-57 1.1976655566623151 1107 58-59 -8.950664448641572 1107 60-61 -10.253184418550685 1107 62-63 -3.8473332468578327 1107 64-65 -5.382828637265643 1107 66-67 -7.142766505998264 1107 68-69 -3.8843041629488013 1107 70-71 -3.806731685403939 1107 72-73 -2.286785477056455 1107 74-75 -4.710711014371331 1107 76-77 -6.721106217021401 1107 78-79 -7.48679706544252 1107 80-81 -2.373458569999581 1107 82-83 -1.1000102962255447 1107 84-85 -0.94241924852032 1107 86-87 -0.9476148784908673 1107 88-89 -0.7671524760150099 1107 90-91 -0.2928819701366159 1107 92-93 -3.164313389423736 1107 94-95 -6.255279865556284 1107 96-97 -6.5053442641096275 1107 98-99 -6.127774016562167 1107 100-101 -7.017941361142912 1108 1 -0.1214035238745339 1108 2 -0.12242499693692821 1108 3 -0.10116988399129667 1108 4 -0.07153935963090419 1108 5 -0.030040406295121613 1108 6 -0.16911242516756886 1108 7 -0.16206165667944106 1108 8 -0.11636950887730535 1108 9 -0.15011150361426218 1108 10-11 -0.05311370359602563 1108 12-13 -0.13443870384249124 1108 14-15 -0.16023162157174653 1108 16-17 -0.137794993839357 1108 18-19 -0.17915676035340056 1108 20-21 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92-93 4.742507366434328 2316 94-95 11.54685679461233 2316 96-97 13.482619546817466 2316 98-99 13.21867218757512 2316 100-101 12.078812597249653 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 36.0 5 91.0 6 215.0 7 511.0 8 1191.0 9 2097.0 10 3482.0 11 5725.0 12 8377.0 13 11788.0 14 14426.0 15 19658.0 16 26690.0 17 36113.0 18 40191.0 19 41215.0 20 42946.0 21 43386.0 22 43447.0 23 36691.0 24 37258.0 25 44774.0 26 63043.0 27 93421.0 28 131503.0 29 178692.0 30 223829.0 31 250432.0 32 254569.0 33 236521.0 34 212089.0 35 192123.0 36 157315.0 37 102674.0 38 40516.0 39 3109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55538911444353 19.82656015461328 14.299420123269005 23.318630607674194 2 15.215594447741514 21.925067049340548 39.27785562112517 23.581482881792773 3 19.384927556074672 25.752985694281367 30.63524436484217 24.226842384801795 4 12.442287138815079 17.375296953222442 38.631782619280294 31.550633288682185 5 12.66375764484772 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21.572033252276547 27.63718181104169 28.844967728721056 21.94581720796071 52-53 22.094870438758363 27.901742238604548 27.915483990666385 22.087903331970708 54-55 21.770522946704652 27.302752865595835 28.284918471671954 22.641805716027555 56-57 21.23587916262436 27.399998491688915 28.95440975049634 22.40971259519038 58-59 20.84374402201598 27.04316380462693 30.002381912161567 22.110710261195525 60-61 21.820565178300274 27.466896785164934 28.896651006468797 21.815887030065994 62-63 21.832853981364888 27.48667519474715 28.544717423360737 22.13575340052722 64-65 21.289046180219568 27.93113725302953 28.580900963064988 22.198915603685915 66-67 21.177634038742095 27.43839902659512 28.616393818032027 22.767573116630757 68-69 21.227715431603826 27.13456298017506 29.167415038582327 22.470306549638792 70-71 21.221686825650103 27.112746208505257 29.081227167193763 22.58433979865088 72-73 20.516563559655165 27.07061780056724 29.75532447399226 22.657494165785327 74-75 20.867825570506852 27.805056224873585 28.82825805051122 22.498860154108343 76-77 19.306988827598097 27.662316230042943 29.577702168898064 23.452992773460892 78-79 20.367774373322323 27.621147589453344 29.100387044070064 22.91069099315427 80-81 21.747641966833616 27.571095007803674 27.84066422038683 22.84059880497588 82-83 21.884183967114197 27.291219216433515 28.002351403255847 22.822245413196434 84-85 21.720997733019328 27.52380675840609 27.764465534644188 22.990729973930396 86-87 21.568986404630504 27.66238486241178 27.750475934080725 23.018152798876987 88-89 21.611085343406582 27.5473875778127 27.36014866811735 23.481378410663368 90-91 21.12705652015834 27.282900756118522 27.990477479342747 23.59956524438039 92-93 20.367172794578995 27.760147494618572 28.733631282759152 23.13904842804328 94-95 19.1832271952219 27.867083211651327 29.066239759085327 23.883449834041446 96-97 18.896059862958612 28.05656308398319 28.74971416856564 24.29766288449256 98-99 19.500487203471582 28.02372910121445 28.28704331380293 24.188740381511042 100-101 19.821219414601625 27.57050724132622 28.188257979417884 24.42001536465427 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 625.0 1 490.0 2 379.0 3 425.0 4 485.0 5 586.0 6 756.5 7 1030.0 8 1326.5 9 1571.5 10 1694.0 11 1801.0 12 1991.5 13 2222.5 14 2427.5 15 2641.5 16 2931.0 17 3208.0 18 3499.0 19 3937.0 20 4570.0 21 5387.0 22 6387.5 23 7821.5 24 9743.5 25 12116.5 26 15288.0 27 19120.0 28 23515.5 29 28484.0 30 33770.5 31 39595.0 32 45974.5 33 52399.5 34 58599.5 35 64717.0 36 70802.5 37 76251.5 38 80366.0 39 83101.5 40 85753.5 41 88484.0 42 90991.5 43 94133.0 44 97618.0 45 102260.0 46 107675.0 47 112717.5 48 115614.0 49 111233.5 50 101908.0 51 93649.0 52 86619.0 53 80344.5 54 74418.5 55 67635.0 56 60491.5 57 52940.0 58 45266.0 59 38301.0 60 31247.0 61 24409.0 62 18585.5 63 13856.5 64 10059.5 65 7140.5 66 5059.5 67 3567.0 68 2370.0 69 1490.0 70 952.0 71 620.0 72 399.5 73 262.0 74 166.0 75 97.0 76 48.0 77 23.0 78 9.0 79 3.5 80 2.0 81 4.0 82 3.0 83 1.0 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024575439098485207 2 0.007922598520012446 3 3.0767372893252215E-4 4 0.0 5 0.1398761690159479 6 0.0 7 3.845921611656527E-5 8 0.0013076133479632192 9 0.0 10-11 0.0 12-13 0.0 14-15 5.76888241748479E-4 16-17 0.0 18-19 9.614804029141318E-5 20-21 0.0 22-23 3.0767372893252215E-4 24-25 0.0 26-27 2.884441208742395E-4 28-29 0.0032113445457332 30-31 0.012153112292834625 32-33 0.0027690635603926997 34-35 0.004345891421171875 36-37 0.10628204373812813 38-39 0.10458983822899924 40-41 0.1175890532763983 42-43 0.12010813193203333 44-45 0.11580069972697803 46-47 0.10876266317764657 48-49 0.10820500454395637 50-51 0.10772426434249932 52-53 0.08595634802052338 54-55 0.07380323572768875 56-57 0.557024056624273 58-59 1.587923344628805 60-61 0.5254874994086896 62-63 0.004576646717871267 64-65 0.12060810174154869 66-67 1.920403267956512 68-69 2.89334451727338 70-71 2.805772882175961 72-73 2.6524552171272737 74-75 2.025396927954735 76-77 1.4216833829649518 78-79 0.9465005382367296 80-81 0.053112177456976634 82-83 2.884441208742395E-4 84-85 9.614804029141318E-5 86-87 2.692145128159569E-4 88-89 1.153776483496958E-4 90-91 0.2506002522155393 92-93 0.39351469930469585 94-95 2.0203780002515233 96-97 2.449736688976858 98-99 1.4252793196718505 100-101 0.9524617167347972 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2600157.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.62065327375334 #Duplication Level Percentage of deduplicated Percentage of total 1 69.21995188401856 32.962993282947366 2 15.356912131500847 14.62612375939396 3 6.098112681533978 8.711883288948234 4 3.038341642367174 5.787512555134938 5 1.737132277354939 4.136168693528253 6 1.1213861197760406 3.2040683757512687 7 0.6983288433753734 2.32784130050079 8 0.5038510315515845 1.919497222011277 9 0.37892847854442485 1.6240339523083445 >10 1.7166281167372288 14.01271495665004 >50 0.08625551133495325 2.7937098479362805 >100 0.039965436686880636 3.39978700255308 >500 0.0016176327761507092 0.49258578915374995 >1k 0.002102922608982587 2.145245969063756 >5k 4.0440819403511294E-4 1.3178759372584785 >10k+ 8.088163880702258E-5 0.5379580668602165 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13967 0.5371598715000672 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8631 0.33194149430207487 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7448 0.2864442416361781 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6461 0.2484849953291282 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6212 0.23890865051610344 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5464 0.21014115686091264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4713 0.1812582855573721 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4172 0.1604518496383103 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3944 0.15168314836373342 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3457 0.13295351011496614 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3358 0.12914604771942617 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2892 0.11122405300910676 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2777 0.10680124315570175 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2748 0.10568592588832135 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.845921611656527E-5 14-15 0.0 0.0 0.0 3.845921611656527E-5 5.76888241748479E-5 16-17 0.0 0.0 0.0 1.3460725640797845E-4 7.691843223313054E-5 18-19 0.0 0.0 0.0 1.5383686446626108E-4 7.691843223313054E-5 20-21 0.0 0.0 0.0 2.884441208742395E-4 7.691843223313054E-5 22-23 0.0 0.0 0.0 8.461027545644359E-4 7.691843223313054E-5 24-25 0.0 0.0 0.0 0.0012499245237883713 7.691843223313054E-5 26-27 0.0 0.0 0.0 0.0020960272783528073 7.691843223313054E-5 28-29 0.0 0.0 0.0 0.005076616527386616 7.691843223313054E-5 30-31 0.0 0.0 0.0 0.013787628977788648 9.614804029141317E-5 32-33 0.0 0.0 0.0 0.03313261468442098 1.153776483496958E-4 34-35 0.0 0.0 0.0 0.06291927756670078 1.153776483496958E-4 36-37 0.0 0.0 0.0 0.10416678685171703 1.153776483496958E-4 38-39 0.0 0.0 0.0 0.16758603422793317 1.153776483496958E-4 40-41 0.0 0.0 0.0 0.23944707954173536 1.153776483496958E-4 42-43 0.0 0.0 0.0 0.31551940902030146 1.153776483496958E-4 44-45 0.0 0.0 0.0 0.40835995672569003 1.153776483496958E-4 46-47 0.0 0.0 0.0 0.513545912804496 1.153776483496958E-4 48-49 0.0 0.0 0.0 0.6095016570153264 1.153776483496958E-4 50-51 0.0 0.0 0.0 0.7015730203983837 1.153776483496958E-4 52-53 0.0 1.9229608058282634E-5 0.0 0.794202042415131 1.153776483496958E-4 54-55 0.0 3.845921611656527E-5 0.0 0.898291910834615 1.153776483496958E-4 56-57 0.0 3.845921611656527E-5 0.0 0.9878441955620372 1.153776483496958E-4 58-59 0.0 3.845921611656527E-5 0.0 1.0448792130629037 1.153776483496958E-4 60-61 0.0 3.845921611656527E-5 0.0 1.0925301818313278 1.153776483496958E-4 62-63 9.614804029141317E-5 3.845921611656527E-5 0.0 1.1326431442409053 1.153776483496958E-4 64-65 1.153776483496958E-4 3.845921611656527E-5 0.0 1.1744290825515535 1.153776483496958E-4 66-67 1.153776483496958E-4 3.845921611656527E-5 0.0 1.2123883288586037 1.153776483496958E-4 68-69 1.5383686446626108E-4 3.845921611656527E-5 0.0 1.2649236180738317 1.153776483496958E-4 70-71 1.5383686446626108E-4 3.845921611656527E-5 0.0 1.3446688026915297 1.3460725640797845E-4 72-73 1.5383686446626108E-4 3.845921611656527E-5 0.0 1.454142961367333 1.5383686446626108E-4 74-75 1.5383686446626108E-4 3.845921611656527E-5 0.0 1.6007494932036797 1.7306647252454373E-4 76-77 1.5383686446626108E-4 3.845921611656527E-5 0.0 1.8279665420203473 1.9229608058282636E-4 78-79 1.5383686446626108E-4 3.845921611656527E-5 0.0 2.184041194435567 2.4998490475767424E-4 80-81 1.5383686446626108E-4 3.845921611656527E-5 0.0 2.6135729496334257 2.692145128159569E-4 82-83 1.5383686446626108E-4 3.845921611656527E-5 0.0 3.0313554143076744 3.0767372893252215E-4 84-85 1.5383686446626108E-4 3.845921611656527E-5 0.0 3.3559511983314856 3.0767372893252215E-4 86-87 1.5383686446626108E-4 3.845921611656527E-5 0.0 3.6094551213638253 3.0767372893252215E-4 88-89 1.5383686446626108E-4 7.691843223313054E-5 0.0 3.847498439517306 3.0767372893252215E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGG 8940 0.0 25.841627 2 GTACATG 9525 0.0 25.10056 1 ACATGGG 9040 0.0 24.46087 3 CATGGGA 5365 0.0 20.783615 4 CATGGGG 4305 0.0 20.218134 4 GAGTACT 7890 0.0 19.591137 12-13 GTACTTT 8115 0.0 18.758282 14-15 AGTACTT 8000 0.0 18.675743 12-13 TACGCGG 120 2.4516303E-6 18.252163 64-65 ATGGGAT 2560 0.0 17.643053 5 AGAGTAC 14065 0.0 17.169756 10-11 GGACCGA 480 1.8189894E-12 16.639494 6 AGTACAT 6930 0.0 16.227087 2 GCTTACG 570 0.0 16.015799 60-61 CGCGTAG 155 1.6031845E-6 15.700471 66-67 TACTTTT 9585 0.0 15.391257 14-15 ACTTTTT 9930 0.0 15.353163 16-17 GAGTACA 9395 0.0 15.278747 1 ATGGGGA 2595 0.0 15.229457 5 GTATCAA 25790 0.0 15.1282215 1 >>END_MODULE