FastQCFastQC Report
Fri 27 May 2016
SRR939658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604396
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54710.21006790058040328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33900.13016453719019688No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29950.11499787282732733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG79800.030.7625061
TACATGG90000.026.9814742
ACATGGG90800.026.0158233
GTATCGA2150.001847561923.3018321
CATGGGA56700.022.1616384
GTATAGA7800.021.8379971
GAGTACA87000.020.1547431
GAGTACT72500.019.98550612-13
AGTACTT77300.019.62981812-13
GTATATA13400.019.441381
GTACTTT75550.018.7767714-15
AGTACAT66250.018.6835982
CATGGGG43850.018.5294344
GTATATG12600.018.2900891
GTATAAT14800.017.602331
GTACTAT6901.0852091E-817.4257161
ATGGGAT29400.017.3378565
AGAGTAC136400.017.00587310-11
GTATAGG7852.9631337E-916.5932791
GTATAAG11850.016.0654811
Produced by FastQC (version 0.11.5)