FastQCFastQC Report
Fri 27 May 2016
SRR939658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604396
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT138060.5301037169462708No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85530.32840627922942595No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT74090.2844805475050645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66150.25399363230476474No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61410.23579363506932124No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC54350.20868562230935694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46520.1786210699140991No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT39360.15112909096773303No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT38380.14736622234099575No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC33330.12797592992770684No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33110.12713120431762295No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28320.10873922398897864No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26910.1033253007607138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG92950.026.710071
TACATGG91300.026.4618992
ACATGGG89300.025.2022133
CATGGGA53800.020.4336244
GAGTACT73700.020.34414712-13
CATGGGG42750.019.2037014
GTACTTT77350.018.92697114-15
AGTACTT78450.018.09061612-13
TTACGCG1102.4543415E-517.62589862-63
AACGCAT8150.017.3036164-65
GTATAAT16500.016.8774471
CGCATAG6600.016.57892666-67
AGAGTAC138050.016.49645210-11
AGTACAT66600.016.0123842
ACGCATA8150.015.81191964-65
ACCGAGT5500.015.4308428
GCTTACG4350.015.3801660-61
ATGGGAG25500.015.3571175
ATGGGAT25000.015.2868065
TCTATAC8350.015.2562933
Produced by FastQC (version 0.11.5)