##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939657_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 624326 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.462023045652433 31.0 31.0 34.0 30.0 34.0 2 31.576418409612927 31.0 31.0 34.0 30.0 34.0 3 31.597208830002273 31.0 31.0 34.0 30.0 34.0 4 35.31692897620794 37.0 35.0 37.0 33.0 37.0 5 35.13441695524453 37.0 35.0 37.0 32.0 37.0 6 35.24282986772936 37.0 35.0 37.0 32.0 37.0 7 35.20667888250689 37.0 35.0 37.0 32.0 37.0 8 35.23271175635806 37.0 35.0 37.0 32.0 37.0 9 36.71347821490696 39.0 35.0 39.0 32.0 39.0 10-11 36.615414062525026 39.0 35.0 39.0 32.0 39.0 12-13 36.53439949641694 38.5 35.0 39.0 32.0 39.0 14-15 37.162724281865565 39.0 36.0 41.0 31.5 41.0 16-17 37.199084292501034 39.0 36.0 40.0 32.0 41.0 18-19 37.48357588823788 39.0 36.0 40.5 32.0 41.0 20-21 37.51335119793185 39.0 36.0 41.0 32.0 41.0 22-23 37.35857869126066 39.0 36.0 41.0 32.0 41.0 24-25 37.1802151440113 39.0 36.0 40.5 31.5 41.0 26-27 36.59752196768996 38.5 35.0 40.0 30.5 41.0 28-29 36.279716846647425 38.0 35.0 40.0 30.0 41.0 30-31 36.02042042138242 38.0 35.0 40.0 30.0 41.0 32-33 35.71715017474845 38.0 35.0 40.0 30.0 41.0 34-35 35.59995579232644 38.0 34.5 40.0 30.0 41.0 36-37 35.21434154592312 37.5 34.0 40.0 27.5 41.0 38-39 34.92487498518402 37.5 33.5 40.0 26.0 41.0 40-41 34.64361487428042 37.0 33.0 40.0 25.0 41.0 42-43 34.51644813767167 37.0 33.0 40.0 24.5 41.0 44-45 34.28161489350116 37.0 33.0 40.0 24.0 41.0 46-47 33.73431828884269 37.0 33.0 40.0 22.5 41.0 48-49 33.30039995130748 37.0 33.0 40.0 20.0 41.0 50-51 33.039021280548944 37.0 33.0 40.0 18.5 41.0 52-53 32.48496058149108 36.0 31.5 40.0 14.0 41.0 54-55 32.281094332127765 36.0 31.5 40.0 10.5 41.0 56-57 30.584940399727067 35.5 27.5 39.0 4.5 40.5 58-59 26.02678728741074 32.0 14.0 37.5 2.0 40.0 60-61 26.686803849271055 32.0 17.5 37.0 2.0 40.0 62-63 29.467039655564562 34.5 27.5 38.0 2.0 40.0 64-65 29.391627130697746 35.0 28.0 38.0 2.0 40.0 66-67 23.11009472615268 25.5 14.5 34.5 2.0 37.5 68-69 16.348948305853032 13.0 6.5 28.0 2.0 34.5 70-71 15.40913401011651 12.0 6.5 25.5 2.0 33.0 72-73 16.477764821583598 13.0 6.5 28.0 2.0 34.0 74-75 17.35043390792631 16.0 6.5 29.0 2.0 34.5 76-77 18.21206228797135 19.5 7.0 29.5 2.0 33.5 78-79 20.991586126478794 25.5 7.5 33.0 2.0 35.0 80-81 24.449228447958276 30.5 16.0 34.0 2.0 35.5 82-83 25.68041295733319 32.5 20.5 35.0 2.0 36.0 84-85 25.749633204447676 33.0 20.0 35.0 2.0 35.5 86-87 25.409808337310956 33.0 19.0 35.0 2.0 35.0 88-89 24.783887744543716 32.5 11.0 35.0 2.0 35.0 90-91 23.431722369403165 31.5 2.0 34.5 2.0 35.0 92-93 21.405619019550684 30.5 2.0 34.0 2.0 35.0 94-95 14.970420101036957 3.5 2.0 31.5 2.0 35.0 96-97 12.660774178874497 2.0 2.0 29.0 2.0 34.0 98-99 12.376598924279943 2.0 2.0 29.0 2.0 34.0 100-101 12.149303408796046 2.0 2.0 29.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.32016678542041177 1101 2 0.27342405387131663 1101 3 0.47333830398552124 1101 4 0.29433751774768524 1101 5 0.32263795560690056 1101 6 0.37205317174841923 1101 7 0.2936789300991691 1101 8 0.31692553523328826 1101 9 0.19350573974401897 1101 10-11 0.3088939410586775 1101 12-13 0.4061899864788714 1101 14-15 0.37893994411800946 1101 16-17 0.3590548061528054 1101 18-19 0.4284167181507357 1101 20-21 0.4962843555574352 1101 22-23 0.6094642413983635 1101 24-25 0.3667743228344946 1101 26-27 0.4926095809698836 1101 28-29 0.4015624363711865 1101 30-31 0.46212514313264563 1101 32-33 0.3418720548384755 1101 34-35 0.233571672324004 1101 36-37 0.2502060191070612 1101 38-39 0.3697838047362012 1101 40-41 0.43088070422323455 1101 42-43 0.5103767844805205 1101 44-45 0.3516368682723936 1101 46-47 0.23277360096706445 1101 48-49 0.3887259075684568 1101 50-51 0.38483006233256134 1101 52-53 0.29607272790841677 1101 54-55 -0.10585020919788235 1101 56-57 -2.101715572444771 1101 58-59 -9.31049584826821 1101 60-61 -10.038816008582462 1101 62-63 -4.0257029695929525 1101 64-65 -4.5424764862641105 1101 66-67 -9.512321636089343 1101 68-69 -4.36036085271024 1101 70-71 -4.041004966443763 1101 72-73 -3.8291641527964835 1101 74-75 -5.668632111421463 1101 76-77 -6.994217652435415 1101 78-79 -10.29901268098953 1101 80-81 -7.597649608263236 1101 82-83 -4.242221564949297 1101 84-85 -3.653547003354088 1101 86-87 -3.2747572020433857 1101 88-89 -3.0683327603789152 1101 90-91 -8.78123135817965 1101 92-93 -15.037004490891627 1101 94-95 -9.780322736206905 1101 96-97 -8.22612284355513 1101 98-99 -8.223669004101891 1101 100-101 -8.386873071043844 1102 1 0.39476104259893674 1102 2 0.35644527734197595 1102 3 0.29828138983409147 1102 4 0.21697099061248792 1102 5 0.34004267030176294 1102 6 0.26993816844372986 1102 7 0.4967706536799881 1102 8 0.3930987011288636 1102 9 0.3469169169489845 1102 10-11 0.38499366933950085 1102 12-13 0.3552610006523267 1102 14-15 0.49711024594660813 1102 16-17 0.45837918009714684 1102 18-19 0.6053646508818815 1102 20-21 0.5573018337097437 1102 22-23 0.7094862726827884 1102 24-25 0.6071356358990485 1102 26-27 0.5613930868816155 1102 28-29 0.6074264299086778 1102 30-31 0.4834312311109059 1102 32-33 0.5937814328218849 1102 34-35 0.6525699832588714 1102 36-37 0.6021833268841021 1102 38-39 0.4436436857672703 1102 40-41 0.6779248402297 1102 42-43 0.7557318553478183 1102 44-45 0.51526505362893 1102 46-47 0.3042375798170269 1102 48-49 0.20284612494378962 1102 50-51 0.42520239103932056 1102 52-53 0.5147699446228202 1102 54-55 -0.13759361817195526 1102 56-57 -2.525992211939517 1102 58-59 -10.000717629864742 1102 60-61 -10.21462565828504 1102 62-63 -4.290179359399811 1102 64-65 -5.232606470842214 1102 66-67 -6.085327804848983 1102 68-69 -4.410601360898534 1102 70-71 -4.1877700395141915 1102 72-73 -4.422163712170796 1102 74-75 -5.247054304268637 1102 76-77 -7.1590575584352685 1102 78-79 -9.4777047570709 1102 80-81 -5.658336617149185 1102 82-83 -3.462240658729133 1102 84-85 -3.2579110336103874 1102 86-87 -2.936081282237261 1102 88-89 -2.945168700747569 1102 90-91 -8.344681457320428 1102 92-93 -11.721993842437488 1102 94-95 -9.347683388332923 1102 96-97 -8.576117335734024 1102 98-99 -8.666929267008552 1102 100-101 -8.753592979981203 1103 1 0.12824452608241899 1103 2 0.08926633369244641 1103 3 0.11964687296427812 1103 4 0.2150278710223077 1103 5 0.19854823468968164 1103 6 0.2431761122699072 1103 7 0.2998089861300812 1103 8 0.24246277108116487 1103 9 0.3645816404960627 1103 10-11 0.2802860205142039 1103 12-13 0.3243035726360759 1103 14-15 0.48379546057299905 1103 16-17 0.4863777669192544 1103 18-19 0.507546244239947 1103 20-21 0.3002580251953475 1103 22-23 0.4082144125873981 1103 24-25 0.4425887360580347 1103 26-27 0.536198774922596 1103 28-29 0.28795990455979137 1103 30-31 0.4418087262531074 1103 32-33 0.45158040826791535 1103 34-35 0.5050253798319133 1103 36-37 0.38921300361966615 1103 38-39 0.46616620828979194 1103 40-41 0.878816908768826 1103 42-43 0.8168959606295729 1103 44-45 0.8404946950350407 1103 46-47 1.081993276690369 1103 48-49 1.0066572890490733 1103 50-51 0.9087741981405415 1103 52-53 0.8945615008849614 1103 54-55 1.1926483885406398 1103 56-57 -2.0101160416515818 1103 58-59 -9.423169493493077 1103 60-61 -9.74960181090825 1103 62-63 -3.5572478985271694 1103 64-65 -4.554281086634479 1103 66-67 -5.3861359659512615 1103 68-69 -4.207655768247058 1103 70-71 -3.5739208962532825 1103 72-73 -3.712770495424637 1103 74-75 -4.841494332532196 1103 76-77 -6.013963935400469 1103 78-79 -8.331220037056767 1103 80-81 -3.786427943769926 1103 82-83 -1.929340111122702 1103 84-85 -1.5498868329391264 1103 86-87 -1.5019265392589887 1103 88-89 -1.7723856235527293 1103 90-91 -3.9818038738546093 1103 92-93 -8.364371514226924 1103 94-95 -7.899882646414534 1103 96-97 -8.121758565198784 1103 98-99 -8.071120222670096 1103 100-101 -7.836418452512557 1104 1 0.28872013429593935 1104 2 0.2934360903078286 1104 3 0.1731577574378136 1104 4 0.2205091713554026 1104 5 0.1721065410918925 1104 6 0.17310723638291137 1104 7 0.1219172281704175 1104 8 0.23344466790871365 1104 9 0.30242964726211596 1104 10-11 0.288639423012782 1104 12-13 0.3785982086504518 1104 14-15 0.5742530375406218 1104 16-17 0.5206644166046388 1104 18-19 0.4070710328626532 1104 20-21 0.31859061859447024 1104 22-23 0.476715785392706 1104 24-25 0.4524141529220813 1104 26-27 0.4904038747641124 1104 28-29 0.6339576872373911 1104 30-31 0.8100132159589322 1104 32-33 0.6198538482675175 1104 34-35 0.4710648799174777 1104 36-37 0.17502065214181783 1104 38-39 0.07343475976317393 1104 40-41 0.04646409160367426 1104 42-43 0.0975869020987048 1104 44-45 -0.2037847867960565 1104 46-47 -0.22773987575178012 1104 48-49 -0.6099170718585896 1104 50-51 -0.25087053423063566 1104 52-53 -0.37187061903894403 1104 54-55 -0.6701764901142866 1104 56-57 -3.2452179417530225 1104 58-59 -9.955241301007668 1104 60-61 -10.327041919641374 1104 62-63 -4.594125144682199 1104 64-65 -5.171871100250918 1104 66-67 -5.302317955043225 1104 68-69 -3.908221755910411 1104 70-71 -4.0175193079163005 1104 72-73 -4.274091612816232 1104 74-75 -4.668150069667881 1104 76-77 -6.203413548361139 1104 78-79 -8.013170685511959 1104 80-81 -4.426393319809449 1104 82-83 -2.8752314920879556 1104 84-85 -2.5979982645506077 1104 86-87 -2.519689920700447 1104 88-89 -2.0811477673612195 1104 90-91 -3.719578217896732 1104 92-93 -5.518920621469434 1104 94-95 -6.453140547795773 1104 96-97 -6.832567887873757 1104 98-99 -7.048901114509903 1104 100-101 -7.182180613682629 1105 1 0.14706846183926459 1105 2 0.17398166356845834 1105 3 0.14069300836861842 1105 4 0.15851288958720033 1105 5 0.09948638388943465 1105 6 0.02668815278251202 1105 7 0.17921338583141733 1105 8 -0.060340502028438436 1105 9 0.07897438317296235 1105 10-11 0.0696811758650071 1105 12-13 0.24850370774581876 1105 14-15 0.29909765432848445 1105 16-17 0.23558793367312347 1105 18-19 0.24472859667319113 1105 20-21 0.23785738926530087 1105 22-23 0.31440076244035 1105 24-25 0.24897927431288025 1105 26-27 0.3458451531181197 1105 28-29 0.5502264466139692 1105 30-31 0.5656030242157968 1105 32-33 0.40131552206993604 1105 34-35 0.5446694215432331 1105 36-37 0.4935649594093192 1105 38-39 0.3730115990667713 1105 40-41 0.1887363000639013 1105 42-43 0.22727020444677493 1105 44-45 0.2388106486507553 1105 46-47 0.15209669151282412 1105 48-49 0.031107834313374383 1105 50-51 0.0722455533521682 1105 52-53 0.15266173605717626 1105 54-55 0.16691785627937605 1105 56-57 1.021912839324866 1105 58-59 -8.196508938933633 1105 60-61 -9.25413890767425 1105 62-63 -3.619892341504258 1105 64-65 -4.569306965736658 1105 66-67 -5.0990861835998444 1105 68-69 -4.111584363258157 1105 70-71 -4.091354369944979 1105 72-73 -2.743398755708977 1105 74-75 -4.346531741675976 1105 76-77 -6.4159458835203615 1105 78-79 -8.280848485275706 1105 80-81 -4.58546236627738 1105 82-83 -3.0829677568101097 1105 84-85 -2.681340735629643 1105 86-87 -2.7322035650161887 1105 88-89 -2.6907496274739593 1105 90-91 -4.292341671565868 1105 92-93 -6.344976155703584 1105 94-95 -7.102303447219098 1105 96-97 -7.035045795296763 1105 98-99 -7.4064806253580695 1105 100-101 -7.153457771478205 1106 1 0.25204993148782506 1106 2 0.23760083289753453 1106 3 0.22028661859226162 1106 4 0.20888733048176533 1106 5 0.16545443500764634 1106 6 0.21273032530647384 1106 7 0.21182424845122938 1106 8 0.3351457599523897 1106 9 0.26742933524963775 1106 10-11 0.520363603980023 1106 12-13 0.252942046403966 1106 14-15 0.3894043635936626 1106 16-17 0.4098563042801473 1106 18-19 0.2946953698361341 1106 20-21 0.20225738926529857 1106 22-23 0.17866274327102616 1106 24-25 0.252029753546104 1106 26-27 0.08412119145678076 1106 28-29 0.1459414625884108 1106 30-31 0.2689985513723414 1106 32-33 0.23671999491338624 1106 34-35 0.44410200940266265 1106 36-37 0.31630489551155705 1106 38-39 0.4742946661594232 1106 40-41 -0.058762741469642776 1106 42-43 0.019603750772652973 1106 44-45 0.036432373890370684 1106 46-47 0.4286807170719271 1106 48-49 0.24757684389803813 1106 50-51 0.03481807731383668 1106 52-53 0.08884576161627677 1106 54-55 0.3108194537234681 1106 56-57 0.9667907945964309 1106 58-59 -8.326071878230756 1106 60-61 -9.970060952402687 1106 62-63 -4.077174130641641 1106 64-65 -5.171414313979469 1106 66-67 -6.783323883280353 1106 68-69 -4.082555609264546 1106 70-71 -3.843069385919419 1106 72-73 -4.129673516092364 1106 74-75 -4.870088291196742 1106 76-77 -6.3038640943829805 1106 78-79 -8.927417175371554 1106 80-81 -5.144445113881215 1106 82-83 -2.9726380443500453 1106 84-85 -2.5662391381855514 1106 86-87 -2.323320497923536 1106 88-89 -2.1372301386560686 1106 90-91 -4.041316751438078 1106 92-93 -5.4926029927642865 1106 94-95 -7.025408878529002 1106 96-97 -7.011289884753632 1106 98-99 -6.733382861779795 1106 100-101 -7.546747228347215 1107 1 0.204326623217149 1107 2 0.17290308853663205 1107 3 0.05934827272759691 1107 4 0.14366026281259536 1107 5 -0.022274888300621853 1107 6 0.11574385914105534 1107 7 0.1447926695038575 1107 8 0.16074575995239115 1107 9 0.22696016231730454 1107 10-11 -0.0015732381252391292 1107 12-13 0.17454204640396398 1107 14-15 0.282216393668854 1107 16-17 0.2261269809718769 1107 18-19 0.24406679840755885 1107 20-21 0.10139122385176336 1107 22-23 0.24239206657929202 1107 24-25 0.0010763700874534265 1107 26-27 0.22031367265978474 1107 28-29 0.3719504851448008 1107 30-31 0.28926321302648006 1107 32-33 0.20585082198105908 1107 34-35 0.27674561842521683 1107 36-37 0.270578579722077 1107 38-39 0.013199929317323722 1107 40-41 0.1132312434927556 1107 42-43 -0.3120233168965214 1107 44-45 -0.02965484415474151 1107 46-47 0.20437395015463977 1107 48-49 0.1003828589356317 1107 50-51 0.3396060472386395 1107 52-53 0.15418711499974336 1107 54-55 0.37954727327234394 1107 56-57 1.2949953058746253 1107 58-59 -8.66177714138865 1107 60-61 -10.58030456142524 1107 62-63 -4.078172626882239 1107 64-65 -4.808605291423078 1107 66-67 -6.6460126050848665 1107 68-69 -3.965081925054019 1107 70-71 -4.223604724265284 1107 72-73 -2.4141547190998836 1107 74-75 -4.613913855106517 1107 76-77 -6.299394921450649 1107 78-79 -7.007748002439223 1107 80-81 -2.473124813129335 1107 82-83 -1.3473087210417738 1107 84-85 -1.0890120705163788 1107 86-87 -1.0247220016829353 1107 88-89 -0.8291519431673464 1107 90-91 -0.5182550973027382 1107 92-93 -2.4924887070500006 1107 94-95 -5.590732186799679 1107 96-97 -5.956925974979196 1107 98-99 -5.909876094862501 1107 100-101 -6.664840461429922 1108 1 0.012373335743145475 1108 2 -0.05456451664653983 1108 3 -0.14786657289710092 1108 4 0.10609827273101047 1108 5 -0.012004531557700204 1108 6 -0.05666055615249377 1108 7 -0.08555447495302815 1108 8 -0.049284635184385195 1108 9 0.011954563213166125 1108 10-11 -0.03616770301086092 1108 12-13 -0.07864944295774023 1108 14-15 -0.10536159385313937 1108 16-17 -0.13427135529431666 1108 18-19 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2316 90-91 3.0178897001748304 2316 92-93 4.8097002330421645 2316 94-95 10.667623379535513 2316 96-97 12.215897212020565 2316 98-99 11.579191331768593 2316 100-101 10.96858180391085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 9.0 4 17.0 5 100.0 6 245.0 7 451.0 8 716.0 9 1201.0 10 1995.0 11 2910.0 12 4132.0 13 5331.0 14 6396.0 15 8275.0 16 11018.0 17 13868.0 18 14076.0 19 12329.0 20 12458.0 21 12450.0 22 12208.0 23 11062.0 24 10860.0 25 12277.0 26 17036.0 27 25005.0 28 33983.0 29 44618.0 30 52696.0 31 56225.0 32 55201.0 33 50644.0 34 45983.0 35 39346.0 36 27186.0 37 15950.0 38 5652.0 39 415.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.77764284821706 18.44923212975453 14.606356357158191 16.166768664870215 2 13.103254949702055 25.162266931505094 40.97568398795413 20.758794130838726 3 22.87715071933573 33.25618346825217 31.081518309344798 12.785147503067307 4 10.51598043329927 17.197906222069882 40.502718131232726 31.78339521339813 5 10.057865766817638 44.55535739488574 32.351582647968286 13.035194190328333 6 22.375169382662264 38.70029439747824 20.685026732828682 18.239509487030816 7 22.779285181139343 40.95616713063367 20.251278979251225 16.013268708975758 8 22.44541652584656 31.38075246788901 22.08294157313809 24.090889433126332 9 27.59760125319144 20.758065497832863 22.420177919868784 29.22415532910691 10-11 21.82946089062445 27.3417253165814 27.222797064354197 23.60601672843995 12-13 31.863641751264566 25.802945896855167 26.264163273674328 16.06924907820594 14-15 26.727970355112372 26.251694834488852 31.80082120887649 15.219513601522289 16-17 16.531988095962685 30.891233105781275 33.81830966514289 18.75846913311315 18-19 15.744098435753115 32.273123336205764 36.665299859368346 15.317478368672777 20-21 18.112572598289997 30.243014066369174 32.74763504963753 18.896778285703302 22-23 16.804616203965097 29.604491268535092 31.255961462225052 22.33493106527476 24-25 18.321598011295382 29.748080329827687 34.432011481181306 17.49831017769563 26-27 21.192567973411283 26.5270492131502 35.808192848276136 16.472189965162375 28-29 17.03900010571739 27.165204242791198 39.15940260064776 16.636393050843658 30-31 15.981901568754974 33.583338806541576 36.03667102921412 14.398088595489329 32-33 15.443837641686308 32.852803212504035 33.50928744591033 18.19407169989933 34-35 17.08456104071677 28.70748942216128 33.997009452979775 20.210940084142173 36-37 19.896229833602167 29.05749046795984 35.34142180520326 15.704857893234736 38-39 22.32214649960838 25.62007521310513 36.75325018899478 15.304528098291712 40-41 16.989260243658975 28.493347284192634 39.479328274142404 15.03806419800599 42-43 15.05673868613841 32.99342772542533 36.00920134476259 15.940632243673674 44-45 15.654406983633711 34.158582940672304 32.100069031371994 18.08694104432199 46-47 15.735007740552398 31.26982739890983 29.466851916137472 23.528312944400305 48-49 18.020127744555783 30.326945571070436 31.280339732854017 20.372586951519764 50-51 21.841664328376158 31.367378843147474 31.360564396520623 15.430392431955747 52-53 16.897616387277353 31.312149227077224 35.350135700327506 16.440098685317913 54-55 14.19991344350586 30.966146793402473 36.47490663118919 18.35903313190248 56-57 17.086482150795337 34.822538768305016 30.4533330756296 17.637646005270042 58-59 16.44825972863565 31.21194491344413 31.71130413556012 20.628491222360097 60-61 16.75648741959399 28.936664495942573 33.21682796837129 21.09002011609215 62-63 23.080607209045663 28.1516548961315 31.182734555076973 17.585003339745857 64-65 20.584563401603493 29.068927389021237 34.61748478334254 15.729024426032732 66-67 18.207132448682206 29.879874118852374 33.84251794949243 18.070475482972984 68-69 19.79728242364082 33.11705068516529 29.86588552079735 17.21978137039654 70-71 19.376948015577536 32.36391072569964 27.998378987460214 20.26076227126261 72-73 17.998573852481737 30.87173472669207 27.708549400713732 23.421142020112462 74-75 19.281932933117808 31.058272056119524 29.531885126267287 20.12790988449538 76-77 18.446583805024165 29.32564657990642 32.88515597270651 19.342613642362913 78-79 16.40736908476549 28.851308748160964 35.09619581911952 19.64512634795402 80-81 15.282562133861543 34.629095882903 32.71570067412345 17.372641309112005 82-83 15.458362664988053 33.607068748593484 30.0138309263357 20.92073766008276 84-85 16.459042598772594 29.703896445043494 30.411940566259105 23.425120389924807 86-87 18.303702641815274 30.28433130527474 32.0475169563264 19.364449096583588 88-89 20.703800104112442 26.905938413486563 33.3175829896288 19.072678492772194 90-91 16.44682806986556 29.04952490295335 35.33525753993264 19.16838948724845 92-93 14.013156202163652 32.700848351535264 33.865080590179964 19.420914856121126 94-95 16.057599670522823 33.17153650602203 29.43607268436462 21.334791139090523 96-97 16.43169177816313 30.899738120425212 27.326571462102144 25.34199863930951 98-99 16.334146155233277 30.311054648599384 29.762927848382994 23.591871347784345 100-101 19.24129517144472 30.799088991495477 30.20282632546159 19.756789511598218 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5435.0 1 3876.5 2 2285.0 3 2312.0 4 2370.5 5 2758.0 6 4063.0 7 6187.5 8 7578.5 9 7606.5 10 7264.5 11 6972.5 12 6770.5 13 6521.0 14 6111.5 15 5644.0 16 5199.5 17 4706.5 18 4299.0 19 3961.5 20 3678.0 21 3532.5 22 3540.5 23 3601.0 24 3751.5 25 4098.5 26 4562.5 27 5112.0 28 5680.0 29 6493.5 30 7564.0 31 8631.5 32 9662.0 33 10774.0 34 12015.5 35 13379.0 36 14782.0 37 15608.5 38 15584.5 39 15039.0 40 14297.5 41 13871.5 42 14172.5 43 15784.5 44 19086.0 45 23808.0 46 30207.0 47 38337.0 48 47452.5 49 42839.0 50 27735.5 51 18059.0 52 11577.5 53 7575.5 54 5326.5 55 4145.0 56 3604.5 57 3268.0 58 3051.0 59 2918.0 60 2663.0 61 2249.0 62 1749.5 63 1189.0 64 761.5 65 520.0 66 374.0 67 286.0 68 219.0 69 176.5 70 148.5 71 131.0 72 119.0 73 96.0 74 86.0 75 61.0 76 33.0 77 20.0 78 11.5 79 10.5 80 5.0 81 2.0 82 1.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02114280039594699 2 0.007367945592526981 3 0.0 4 0.0 5 0.1303806024416731 6 0.0 7 0.0 8 0.0011212091119062798 9 0.0 10-11 0.0 12-13 0.0 14-15 4.0043182568081417E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.606045559531399E-4 24-25 0.0 26-27 1.601727302723257E-4 28-29 0.0032034546054465133 30-31 0.011692609309879774 32-33 0.002482677319221048 34-35 0.004084404621944305 36-37 0.10251054737428843 38-39 0.10114907916697366 40-41 0.11348237939794274 42-43 0.11556462489148298 44-45 0.11156030663467484 46-47 0.10611443380541576 48-49 0.10563391561459877 50-51 0.104272447407284 52-53 0.08409068339297097 54-55 0.07303876500418051 56-57 0.5538773012817022 58-59 1.575859406784276 60-61 0.5095895413614041 62-63 0.004324663717352794 64-65 0.12004946133910809 66-67 1.897005730980289 68-69 2.8617260854105067 70-71 2.7706678882506894 72-73 2.6259518264496435 74-75 2.0003972283710754 76-77 1.3969464670700884 78-79 0.9283611446583996 80-81 0.05293708735500364 82-83 8.008636513616285E-5 84-85 8.008636513616285E-5 86-87 2.402590954084885E-4 88-89 1.601727302723257E-4 90-91 0.24962920012941958 92-93 0.38665697087739415 94-95 1.993750060064774 96-97 2.4148441679507178 98-99 1.4055157081396576 100-101 0.9434974676691344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 624326.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.843030592817602 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80171910532928 21.575082725503613 2 11.717948755871328 6.759623089013314 3 4.589550704095282 3.9712965409652337 4 2.437119231676008 2.811756182303009 5 1.4293689452038814 2.0613666107469486 6 1.0121609301328374 1.751627320360569 7 0.6439854742974582 1.3002144915544218 8 0.49602181464481165 1.1445417899604162 9 0.3775085666826095 0.9799642024089508 >10 2.1545586205739724 11.466639276933535 >50 0.19138655589710038 3.821716508118149 >100 0.11538667717854151 6.314432500580885 >500 0.011094872805628991 2.3760724364896144 >1k 0.014978078287599138 10.121908574567879 >5k 0.004437949122251596 10.548161569235107 >10k+ 0.0027737182014072478 12.995596181258348 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22824 3.6557823957355615 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 20039 3.209701341927134 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 14015 2.244820814766644 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13938 2.2324875145356753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10404 1.6664370857532764 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 9904 1.5863507206171135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9213 1.4756713639989365 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 9080 1.4543683908727172 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8905 1.4263381630750602 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 8545 1.368675980177023 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 7385 1.182875613061125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7217 1.1559665943753743 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5675 0.9089802442954481 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4858 0.7781191236629581 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4203 0.6732059853345849 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 4066 0.6512623212872762 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3872 0.620188811614445 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3716 0.5952018656919622 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3449 0.5524357467092513 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3247 0.5200808551942415 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3232 0.5176782642401565 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3101 0.4966956365744819 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2737 0.4383927627553553 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 0.37320246153451886 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2257 0.36150985222463905 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 2242 0.35910726127055415 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 2134 0.341808606401143 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1716 0.27485640514731086 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1569 0.251311013797279 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1560 0.24986945922482803 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1545 0.24746686827074316 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1466 0.23481322257922943 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1462 0.23417253165814014 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 1386 0.22199940415744338 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1382 0.22135871323635406 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1249 0.20005574011013474 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 1208 0.19348865816896943 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1153 0.18467915800399148 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1064 0.17042378500975452 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1056 0.16914240316757592 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 994 0.15921169389069173 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 958 0.153445475600888 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 944 0.15120305737707543 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 938 0.15024202099544148 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 896 0.1435147663240038 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 893 0.14303424813318683 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 890 0.14255372994236984 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 875 0.14015113898828496 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 764 0.12237196592805681 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 706 0.11308194757226192 No Hit GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 699 0.11196073846035566 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 659 0.10555382924946262 No Hit CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 637 0.10203002918347147 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.80518190816977E-4 0.0 12-13 0.0 0.0 0.0 9.61036381633954E-4 0.0 14-15 0.0 0.0 0.0 0.0012012954770424427 0.0 16-17 0.0 0.0 0.0 0.001922072763267908 0.0 18-19 0.0 0.0 0.0 0.0025627636843572107 0.0 20-21 0.0 0.0 0.0 0.004084404621944305 0.0 22-23 0.0 0.0 0.0 0.00848915470443326 0.0 24-25 0.0 0.0 0.0 0.014815977550190124 0.0 26-27 0.0 0.0 0.0 0.020822454935402336 0.0 28-29 0.0 0.0 0.0 0.03203454605446514 0.0 30-31 0.0 0.0 0.0 0.06254745117134318 0.0 32-33 0.0 0.0 0.0 0.10499322469350947 0.0 34-35 0.0 0.0 0.0 0.19284796724788011 0.0 36-37 0.0 0.0 0.0 0.3262718515647274 0.0 38-39 0.0 0.0 0.0 0.4553710721642219 0.0 40-41 0.0 0.0 0.0 0.6056130931596634 0.0 42-43 0.0 0.0 0.0 0.7464049230690377 0.0 44-45 0.0 0.0 0.0 0.8940040940149858 0.0 46-47 0.0 0.0 0.0 1.0812460157033343 0.0 48-49 0.0 0.0 0.0 1.2847454695143243 0.0 50-51 0.0 0.0 0.0 1.4494831225994114 0.0 52-53 0.0 0.0 0.0 1.5813853659786714 0.0 54-55 0.0 0.0 0.0 1.7083222547194894 0.0 56-57 0.0 0.0 0.0 1.8080297793140123 0.0 58-59 0.0 0.0 0.0 1.9012503083325059 0.0 60-61 0.0 0.0 0.0 2.014972946825857 0.0 62-63 0.0 0.0 0.0 2.0962606074390626 0.0 64-65 0.0 0.0 0.0 2.1430310446785814 0.0 66-67 0.0 0.0 0.0 2.1829140545163908 0.0 68-69 0.0 0.0 0.0 2.2428987420033764 0.0 70-71 0.0 0.0 0.0 2.3442880802657586 0.0 72-73 0.0 0.0 0.0 2.5186360971671853 0.0 74-75 0.0 0.0 0.0 2.7204537373103155 0.0 76-77 0.0 0.0 0.0 2.949500741599741 0.0 78-79 0.0 0.0 0.0 3.3303114078221956 0.0 80-81 0.0 0.0 0.0 3.7856824799864173 0.0 82-83 0.0 0.0 0.0 4.241293811246047 0.0 84-85 0.0 0.0 0.0 4.794049903415844 0.0 86-87 0.0 0.0 0.0 5.238769489016956 0.0 88-89 0.0 0.0 0.0 5.488158430050968 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCC 80 2.4332076E-7 41.174213 5 CGCCAAC 25 0.001467539 38.751926 92-93 TAGGACG 105 5.052243E-8 35.85238 4 CGTCACT 80 1.1483502E-5 35.292183 1 CGACGCG 35 1.7835178E-4 34.752068 68-69 CGCGTCG 50 1.0891381E-6 34.009064 72-73 CTGCGCG 95 0.0 33.2764 62-63 GGACGTG 100 1.4169473E-6 32.878696 6 GACGCGG 60 1.2272358E-7 32.41375 70-71 CGGCTTG 310 0.0 30.528263 74-75 GTAGCGA 40 3.9019747E-4 30.411842 64-65 TGCGTAA 40 3.9133718E-4 30.396711 62-63 TTGGACG 65 2.4590918E-7 29.943972 64-65 GTAGCAA 595 0.0 29.440708 64-65 CTGCGGG 215 0.0 29.407051 62-63 ACTGCGG 210 0.0 28.94925 62-63 CCGCTTG 120 0.0 28.310396 74-75 ATGGGAG 1480 0.0 28.297337 5 ACTGCGC 95 3.0013325E-10 28.156954 62-63 GCGTTCG 35 0.007478705 27.79128 62-63 >>END_MODULE