##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939656_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590264 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 15.447574305734383 2.0 2.0 31.0 2.0 33.0 2 22.69547185666075 16.0 16.0 31.0 16.0 34.0 3 27.191061965493407 28.0 25.0 31.0 16.0 34.0 4 32.38863627122779 35.0 32.0 35.0 26.0 37.0 5 32.982587452394185 35.0 33.0 35.0 28.0 37.0 6 33.537264681566214 35.0 33.0 37.0 28.0 37.0 7 33.7577626282477 35.0 33.0 37.0 28.0 37.0 8 33.766960207635904 35.0 35.0 37.0 28.0 37.0 9 35.01629101554558 37.0 34.0 39.0 28.0 39.0 10-11 34.84775456405947 37.0 34.0 39.0 27.0 39.0 12-13 34.81270838133445 37.0 34.0 39.0 27.0 39.0 14-15 35.15193116978166 38.0 33.5 40.0 27.0 41.0 16-17 34.96842853367307 38.0 33.0 40.0 26.5 41.0 18-19 35.60435245923858 38.0 34.0 40.0 27.0 41.0 20-21 35.69212166081618 38.0 34.0 40.0 27.0 41.0 22-23 35.57423457978125 38.0 34.0 40.0 27.0 41.0 24-25 35.278188234417144 38.0 34.0 40.0 26.5 41.0 26-27 35.04120613827033 38.0 33.5 40.0 25.5 41.0 28-29 34.69143213883957 38.0 33.5 40.0 25.0 41.0 30-31 34.44409789517911 37.5 33.0 40.0 24.5 41.0 32-33 34.026212000054215 37.0 33.0 40.0 24.0 41.0 34-35 33.7478170784598 37.0 33.0 40.0 22.5 41.0 36-37 33.20764098776141 36.0 32.5 40.0 19.5 41.0 38-39 32.77776300096228 35.5 31.5 39.0 17.0 41.0 40-41 32.234491685076506 35.0 31.0 39.0 15.0 40.5 42-43 32.021556456094224 35.0 31.0 39.0 15.0 40.0 44-45 31.710479548134394 35.0 30.5 39.0 10.5 40.0 46-47 31.09464493853598 35.0 30.0 39.0 7.5 40.0 48-49 30.590278248376997 35.0 28.5 39.0 2.0 40.0 50-51 29.885321652684222 34.5 27.0 38.5 2.0 39.5 52-53 29.734003767805593 35.0 27.5 38.0 2.0 40.0 54-55 29.91209103045417 35.0 28.0 38.0 2.0 40.0 56-57 29.849140384641448 35.0 27.5 38.0 2.0 40.0 58-59 29.61264366452977 35.0 27.0 38.0 2.0 40.0 60-61 28.9998543024816 34.5 26.0 38.0 2.0 40.0 62-63 28.394426900505536 34.0 26.0 37.5 2.0 40.0 64-65 27.564444045376305 33.0 22.5 36.5 2.0 39.0 66-67 27.052864480978002 33.0 22.0 36.0 2.0 39.0 68-69 26.993694346936287 33.0 22.0 36.0 2.0 39.0 70-71 26.45198758521611 32.5 21.5 35.5 2.0 38.0 72-73 25.821564588048737 32.0 20.5 35.0 2.0 37.0 74-75 25.252844489923152 31.0 18.5 35.0 2.0 37.0 76-77 24.49702082458019 31.0 14.0 34.5 2.0 36.0 78-79 23.859188430939376 30.5 8.5 34.0 2.0 36.0 80-81 23.237690253852513 30.0 4.5 34.0 2.0 35.0 82-83 22.694396913923264 29.0 2.0 34.0 2.0 35.0 84-85 22.113431447623434 29.0 2.0 34.0 2.0 35.0 86-87 21.215638256780018 28.5 2.0 33.0 2.0 35.0 88-89 20.167603817952646 26.5 2.0 33.0 2.0 35.0 90-91 19.140707378393397 25.0 2.0 32.0 2.0 34.0 92-93 18.36309854573547 24.5 2.0 32.0 2.0 34.0 94-95 17.59776218776683 22.0 2.0 32.0 2.0 34.0 96-97 16.629198968597102 19.0 2.0 31.0 2.0 34.0 98-99 15.581264485043981 2.0 2.0 31.0 2.0 34.0 100-101 13.969051136440644 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 14.783388015133447 1101 2 7.8767135982485605 1101 3 3.830369199359506 1101 4 2.4155526361076625 1101 5 1.6773188157896044 1101 6 1.2305876411749992 1101 7 0.7523897978910625 1101 8 0.9543573838734858 1101 9 0.8722128772540927 1101 10-11 0.8695563632540058 1101 12-13 0.858742884669212 1101 14-15 0.792804804343568 1101 16-17 1.0728220221088804 1101 18-19 0.5997982957337911 1101 20-21 0.893352421551711 1101 22-23 0.6437258107481227 1101 24-25 1.1165110096596464 1101 26-27 0.9827644724113256 1101 28-29 1.1334636038692167 1101 30-31 1.1915106646915845 1101 32-33 1.109574538801617 1101 34-35 1.150983376439406 1101 36-37 1.2518231611378141 1101 38-39 1.0296741592656247 1101 40-41 0.7586916731580047 1101 42-43 0.8797797911914138 1101 44-45 0.7791823094686237 1101 46-47 0.9410260218189137 1101 48-49 0.991768462405858 1101 50-51 0.9620451133534189 1101 52-53 1.0067313207994921 1101 54-55 1.1528304702920416 1101 56-57 1.1035713798113385 1101 58-59 1.0358156466618418 1101 60-61 1.1411632022791771 1101 62-63 1.0177975251927194 1101 64-65 1.0409618831216783 1101 66-67 0.6812602528118283 1101 68-69 0.5080302837783144 1101 70-71 0.1826176598677698 1101 72-73 0.10915192993222078 1101 74-75 0.27122887592565803 1101 76-77 0.06920421346711692 1101 78-79 -0.30356473284145125 1101 80-81 -0.2408756046226017 1101 82-83 -0.4205748321408649 1101 84-85 -0.6058803480952193 1101 86-87 -0.7585445532760318 1101 88-89 -0.8923007441352553 1101 90-91 -0.7360685859327951 1101 92-93 -0.4088420493857683 1101 94-95 -0.4433335063169679 1101 96-97 -1.2786016890935148 1101 98-99 -1.3940212518685087 1101 100-101 -1.0440509358672596 1102 1 14.91513240286945 1102 2 8.139565963572942 1102 3 4.128333934750149 1102 4 2.3296672148914936 1102 5 1.4415101381500577 1102 6 1.2775347442609686 1102 7 1.065471146082288 1102 8 0.8834737720587214 1102 9 1.235086456250059 1102 10-11 1.0193198932089587 1102 12-13 0.7803296673039526 1102 14-15 0.6198060646852284 1102 16-17 1.0040635210419566 1102 18-19 1.0104762597204626 1102 20-21 0.994642711929437 1102 22-23 0.9204456779755006 1102 24-25 0.7487857391853296 1102 26-27 0.6455619317621881 1102 28-29 1.203235369720197 1102 30-31 0.9647627205459415 1102 32-33 0.9284859030902766 1102 34-35 0.7658653909894824 1102 36-37 0.8121448602281234 1102 38-39 0.9280918689378126 1102 40-41 0.7787061483692028 1102 42-43 0.6649601697930514 1102 44-45 0.554135202639074 1102 46-47 0.37800394712183305 1102 48-49 0.4623750486269529 1102 50-51 0.27519384614851106 1102 52-53 0.5377120163583449 1102 54-55 0.2700784331556356 1102 56-57 0.3923593304458741 1102 58-59 0.40675541231316714 1102 60-61 0.553102755793784 1102 62-63 0.3036954250392334 1102 64-65 0.35643039329800175 1102 66-67 0.6217396817897018 1102 68-69 0.028956946867769062 1102 70-71 0.17744901117865197 1102 72-73 0.2272883712764191 1102 74-75 0.22675129378507108 1102 76-77 -0.11407566973290528 1102 78-79 -0.10755664668898746 1102 80-81 -0.07189747966160098 1102 82-83 0.02717589473935078 1102 84-85 -0.338302325458983 1102 86-87 -0.34720522188104397 1102 88-89 -0.20067889654924187 1102 90-91 8.881256985375785E-4 1102 92-93 0.38762400250037743 1102 94-95 0.3842430188836268 1102 96-97 0.1201317299264133 1102 98-99 -0.577918827270631 1102 100-101 -0.8218165123175893 1103 1 14.934989055403264 1103 2 7.819270183237794 1103 3 3.597014532367755 1103 4 2.2267548385539158 1103 5 1.5632040610250826 1103 6 1.0103633329950696 1103 7 0.8370666437604584 1103 8 0.8057506749458554 1103 9 0.8037140556847433 1103 10-11 0.9409911025770228 1103 12-13 0.4202600125492566 1103 14-15 0.589671550249534 1103 16-17 0.8072497213246166 1103 18-19 0.7588673863104063 1103 20-21 0.6999488395288722 1103 22-23 0.6851842202731007 1103 24-25 0.8667268859650648 1103 26-27 1.0896816571811243 1103 28-29 1.298673740404631 1103 30-31 1.0197937623387858 1103 32-33 1.1165613119334026 1103 34-35 0.8748813913932807 1103 36-37 0.7261300712113652 1103 38-39 0.44114582613546816 1103 40-41 0.6106691001156221 1103 42-43 1.0303700224076344 1103 44-45 1.098627177199944 1103 46-47 1.2655985867341855 1103 48-49 1.315948640389525 1103 50-51 0.7745667009839607 1103 52-53 0.8431859715370287 1103 54-55 0.7683484210417681 1103 56-57 0.8188508507366059 1103 58-59 0.6035931873989746 1103 60-61 0.8674968099024056 1103 62-63 0.5010442540317612 1103 64-65 0.5470686428437332 1103 66-67 0.5681067825367236 1103 68-69 0.45273420307612966 1103 70-71 0.1505562189315306 1103 72-73 -0.21361108359941383 1103 74-75 -0.24631805812286345 1103 76-77 0.05845865289984431 1103 78-79 0.1340330464262962 1103 80-81 -0.4436431388580431 1103 82-83 -0.5060123245217021 1103 84-85 -0.3762666904902794 1103 86-87 -0.31419492509122193 1103 88-89 -0.6381978244717175 1103 90-91 -0.36438292215125045 1103 92-93 0.1439257388217996 1103 94-95 -0.1705280794406221 1103 96-97 -0.6287098812183451 1103 98-99 -0.5805498084941334 1103 100-101 -0.810810556331317 1104 1 14.671205499366188 1104 2 7.82948746986353 1104 3 3.62288127126266 1104 4 2.288675507868952 1104 5 1.3729729306531127 1104 6 1.106366703646323 1104 7 0.781518611980033 1104 8 0.5834306436469561 1104 9 0.7405218683728378 1104 10-11 0.36752143843833807 1104 12-13 0.3147917327852028 1104 14-15 0.33316724665016295 1104 16-17 0.5417996490963759 1104 18-19 0.31663869364157193 1104 20-21 0.36383547128522764 1104 22-23 0.5168623230779588 1104 24-25 0.6908285472146076 1104 26-27 0.4682930692432379 1104 28-29 0.7142306502397062 1104 30-31 0.45290363955077595 1104 32-33 0.5955173128964475 1104 34-35 0.9466017778143723 1104 36-37 1.2058271640246474 1104 38-39 0.8215215634654314 1104 40-41 0.7257000980691544 1104 42-43 0.7274778832478894 1104 44-45 0.5478680282893862 1104 46-47 0.7289164210907586 1104 48-49 0.7338191110768975 1104 50-51 0.6157209985643135 1104 52-53 0.7216694794219443 1104 54-55 0.7011949661242305 1104 56-57 0.9967806087719957 1104 58-59 0.6869791871582116 1104 60-61 0.5443920488575031 1104 62-63 0.1697385780958065 1104 64-65 0.5803124432530247 1104 66-67 0.9033398690902352 1104 68-69 0.41034149210947746 1104 70-71 0.47985680879549975 1104 72-73 0.4370600371421318 1104 74-75 0.43713415085751706 1104 76-77 0.2847512374670771 1104 78-79 0.057046017414620565 1104 80-81 -0.10143551275957563 1104 82-83 -0.39061642347439474 1104 84-85 -0.5725093171906508 1104 86-87 -0.5529092623971117 1104 88-89 -0.6648207027671305 1104 90-91 -0.10862723417725562 1104 92-93 0.034838523220500406 1104 94-95 -0.12874479425944685 1104 96-97 -0.658270793701794 1104 98-99 -1.0537237882702257 1104 100-101 -0.762771252130042 1105 1 14.875024758097197 1105 2 7.9842734289372395 1105 3 3.803157018573529 1105 4 2.09611543576262 1105 5 1.3423747848160659 1105 6 0.9983136737935112 1105 7 0.6882310384375145 1105 8 0.7506346411905866 1105 9 0.972064428940179 1105 10-11 1.1192002879064589 1105 12-13 0.9454547545161418 1105 14-15 0.9677042506714812 1105 16-17 0.800606180266513 1105 18-19 0.9420769773522792 1105 20-21 0.9731860190160546 1105 22-23 0.8578148596758908 1105 24-25 0.9782572332549009 1105 26-27 0.9518611443606133 1105 28-29 1.3007124453705572 1105 30-31 1.0433757973923505 1105 32-33 0.8989371639295101 1105 34-35 1.0008449584169696 1105 36-37 1.4718802334963357 1105 38-39 1.222314683649266 1105 40-41 1.1203250559741065 1105 42-43 0.70675350179744 1105 44-45 0.8915616952135679 1105 46-47 0.9995628028977706 1105 48-49 0.7575260503213634 1105 50-51 0.560482393940795 1105 52-53 0.5799800319070343 1105 54-55 0.6053433090651374 1105 56-57 0.6543547455759438 1105 58-59 0.8600034438092301 1105 60-61 0.7952698543684846 1105 62-63 0.7363676068392913 1105 64-65 0.6943726837395481 1105 66-67 0.6049961853280266 1105 68-69 0.5864671631540261 1105 70-71 0.3003858197104954 1105 72-73 0.2936917401591046 1105 74-75 0.2183241035129697 1105 76-77 0.25279490736268073 1105 78-79 0.48835185525459934 1105 80-81 0.3755409734431545 1105 82-83 0.5744100221189967 1105 84-85 -0.017109760921954376 1105 86-87 0.5028084828931974 1105 88-89 0.5339966740677191 1105 90-91 0.6861891323764979 1105 92-93 0.8893209027296223 1105 94-95 1.1876044262393535 1105 96-97 0.07808174487707831 1105 98-99 -0.3678246380949126 1105 100-101 -0.4581653112810091 1106 1 14.878213922811646 1106 2 7.7954286152671415 1106 3 3.7193627000114127 1106 4 2.5685670569213457 1106 5 1.8138420958777388 1106 6 1.089738856383029 1106 7 0.6955001685042532 1106 8 1.1875871186990992 1106 9 1.14946663773204 1106 10-11 0.6678448929028136 1106 12-13 0.28506142420136626 1106 14-15 0.745273546102311 1106 16-17 0.8325654987302045 1106 18-19 0.8141708017907305 1106 20-21 0.6009076364343997 1106 22-23 0.7302753396254928 1106 24-25 0.8171072145530047 1106 26-27 0.7457958255425723 1106 28-29 0.9840315882333144 1106 30-31 0.6884704880236754 1106 32-33 0.572290167059613 1106 34-35 0.9065825019727001 1106 36-37 0.9554201078983695 1106 38-39 1.136183858994265 1106 40-41 1.118921183698184 1106 42-43 0.9470715816053037 1106 44-45 0.8223942246936602 1106 46-47 0.94171489849397 1106 48-49 1.070315191412373 1106 50-51 0.8019336607478884 1106 52-53 0.7921406910010766 1106 54-55 0.9278808112798345 1106 56-57 0.5501359924999747 1106 58-59 0.7685376584282047 1106 60-61 1.0956653009499746 1106 62-63 0.8795945690614708 1106 64-65 0.921160190875792 1106 66-67 0.815962138041769 1106 68-69 0.9627196977525294 1106 70-71 0.920026251232045 1106 72-73 0.5143617600421706 1106 74-75 0.4388341730052616 1106 76-77 0.4430017601295759 1106 78-79 0.37123371835674845 1106 80-81 0.5443550766382259 1106 82-83 0.6120303540283629 1106 84-85 0.41392701290326883 1106 86-87 0.3069320039672796 1106 88-89 0.40869399871287726 1106 90-91 0.3578700387292386 1106 92-93 0.9842649939161063 1106 94-95 0.827324783740778 1106 96-97 0.25186046212416 1106 98-99 0.024602572173410664 1106 100-101 -0.1080375970459162 1107 1 14.521968589630507 1107 2 7.7612544222403805 1107 3 3.6108642147954164 1107 4 1.7468383483163308 1107 5 1.0981958276682278 1107 6 1.0572934670811662 1107 7 0.6276012238592585 1107 8 0.6006665770865283 1107 9 0.1358858976042967 1107 10-11 0.26301827265464794 1107 12-13 0.05666909276567367 1107 14-15 0.4156463208917387 1107 16-17 0.6837861245238628 1107 18-19 0.6125856290768112 1107 20-21 0.46745207406192435 1107 22-23 0.35626161382755583 1107 24-25 0.6315616307309995 1107 26-27 0.6628703186725389 1107 28-29 0.6023155305716514 1107 30-31 0.58174242147782 1107 32-33 0.8277322128978781 1107 34-35 0.5364389660815903 1107 36-37 0.4471589467700525 1107 38-39 0.763195049847532 1107 40-41 0.9218458820492827 1107 42-43 1.086033395731551 1107 44-45 0.8190091461484172 1107 46-47 0.8850484220670509 1107 48-49 0.8144783980935912 1107 50-51 1.3173728054299758 1107 52-53 0.9681124594222297 1107 54-55 0.863421639108104 1107 56-57 1.0962120598638698 1107 58-59 0.993604361240422 1107 60-61 1.0419190046801035 1107 62-63 0.9981258230743428 1107 64-65 0.9277805341634213 1107 66-67 0.6031647100831847 1107 68-69 0.7789148076397616 1107 70-71 0.616681836433294 1107 72-73 0.9317370697762613 1107 74-75 1.1075839649305337 1107 76-77 0.5564738258003388 1107 78-79 0.9346608398866607 1107 80-81 0.8932702344658772 1107 82-83 0.7821772191150878 1107 84-85 0.513185791278719 1107 86-87 0.06259877050981189 1107 88-89 -0.07783702701229345 1107 90-91 -0.048854223021635335 1107 92-93 0.0997155529831737 1107 94-95 0.6863511842620369 1107 96-97 0.03829494117638532 1107 98-99 0.007039876285917401 1107 100-101 0.6018717537133771 1108 1 14.688592978362028 1108 2 7.799227054323698 1108 3 3.655312417278541 1108 4 0.285466288394403 1108 5 0.6608940054892258 1108 6 0.7575569995435742 1108 7 0.8476790152599278 1108 8 0.9465916887707451 1108 9 0.8570417763511742 1108 10-11 0.7681726575811538 1108 12-13 0.2850685910675068 1108 14-15 0.6226496608486158 1108 16-17 0.8872486874122956 1108 18-19 0.37384212930934524 1108 20-21 0.46569753975337846 1108 22-23 0.05629966396918462 1108 24-25 0.3522594985138028 1108 26-27 0.5555477803053108 1108 28-29 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-7.286382497414888 2312 3 -3.160153299800079 2312 4 -1.326333739534661 2312 5 -1.839126941305917 2312 6 -0.19837792574619328 2312 7 0.12678109597491272 2312 8 0.3121900688852506 2312 9 0.12839354286423088 2312 10-11 0.173304622390539 2312 12-13 -0.3954159781267421 2312 14-15 0.27622458055257226 2312 16-17 0.17104634137123753 2312 18-19 0.3661371998302201 2312 20-21 0.4519738978039314 2312 22-23 0.7570956421845239 2312 24-25 0.9249728067109828 2312 26-27 0.6821641746636402 2312 28-29 0.05594355392444328 2312 30-31 0.2255775618725906 2312 32-33 0.5034202862923749 2312 34-35 -0.20619378343189254 2312 36-37 0.5688019826332749 2312 38-39 0.8989401151797622 2312 40-41 1.0253835523300765 2312 42-43 0.5468618638994691 2312 44-45 0.2855298578526231 2312 46-47 0.38021105759666085 2312 48-49 0.6195034189443014 2312 50-51 0.5107700809373412 2312 52-53 0.4408258316768858 2312 54-55 0.19397487791822599 2312 56-57 0.24335551274127099 2312 58-59 0.555836777142563 2312 60-61 0.49338550454109864 2312 62-63 0.4427254894629975 2312 64-65 0.4215878736129639 2312 66-67 0.17252111820105753 2312 68-69 0.4695319886239169 2312 70-71 0.2810340744093871 2312 72-73 0.02088775089941919 2312 74-75 0.8778369535321495 2312 76-77 0.8235227554639479 2312 78-79 1.1180219741652238 2312 80-81 1.2056138541489467 2312 82-83 1.3825803328209538 2312 84-85 1.1759186241950381 2312 86-87 1.3506652147689167 2312 88-89 1.7670614995376077 2312 90-91 2.062627567359236 2312 92-93 2.602870960267019 2312 94-95 2.2859550207924784 2312 96-97 2.177146762138296 2312 98-99 2.3182992591049345 2312 100-101 1.7068759238551632 2313 1 -14.302190431776221 2313 2 -7.5105527715851625 2313 3 -3.9296338192805997 2313 4 -1.122726235927157 2313 5 -0.5422293944083734 2313 6 -1.1998930772613434 2313 7 -0.48959697040315575 2313 8 0.012695119390301102 2313 9 -2.4406540761833853 2313 10-11 -0.5467530976671853 2313 12-13 -0.743323626034389 2313 14-15 -1.1485228941949046 2313 16-17 -0.8239392286143357 2313 18-19 -0.26499555630253013 2313 20-21 -1.1518139809839454 2313 22-23 -1.904376219287343 2313 24-25 -1.3455899638517934 2313 26-27 -0.4560753635758985 2313 28-29 0.015683813664701063 2313 30-31 -1.2622651509701228 2313 32-33 -0.08308765021556042 2313 34-35 -0.8698301470682566 2313 36-37 -1.2959166320853406 2313 38-39 -0.7508795096398657 2313 40-41 -0.2120262600797389 2313 42-43 -0.08791447087686421 2313 44-45 0.009303306626069485 2313 46-47 -0.6764267490411484 2313 48-49 0.01881799325887812 2313 50-51 -1.3168633966961352 2313 52-53 -0.17230548145442626 2313 54-55 -1.20905542511208 2313 56-57 -0.7243573299715749 2313 58-59 -0.4719410006352156 2313 60-61 -1.5999406137850194 2313 62-63 -0.5363870646495563 2313 64-65 -0.46531688829179885 2313 66-67 -1.8660430953631533 2313 68-69 -0.8285921095001818 2313 70-71 -0.44425452587921654 2313 72-73 -1.0188308638191934 2313 74-75 -0.625698400003202 2313 76-77 -0.22925502231382922 2313 78-79 -0.21466200851875783 2313 80-81 -0.31401096547587315 2313 82-83 -0.11951201927139721 2313 84-85 0.20427360255001403 2313 86-87 -0.02787013876643485 2313 88-89 -0.015189582713475147 2313 90-91 0.10541256014423084 2313 92-93 0.8983976557937154 2313 94-95 1.023725583563042 2313 96-97 0.14929683428836782 2313 98-99 0.3741434149490903 2313 100-101 -0.17068540370616603 2314 1 -14.302190431776221 2314 2 -7.282570180699345 2314 3 -3.5806322831822897 2314 4 -1.531661207765353 2314 5 -1.1556958562619286 2314 6 -5.3084952277989785 2314 7 -1.6378816606233286 2314 8 -3.5661579272046815 2314 9 -0.8038645216518958 2314 10-11 -0.08209615962314842 2314 12-13 -2.763548920453225 2314 14-15 -2.2175193099655175 2314 16-17 -1.895815828716735 2314 18-19 -0.6824942762206092 2314 20-21 -1.561491400338781 2314 22-23 -1.0939307507773606 2314 24-25 -2.5340692265246005 2314 26-27 -4.195719500800678 2314 28-29 -1.5910750189005753 2314 30-31 -1.544241597462694 2314 32-33 -3.9019560577936474 2314 34-35 -2.5381097169607294 2314 36-37 -0.8285587211790428 2314 38-39 -1.2150628173715603 2314 40-41 -1.9607845806122555 2314 42-43 -1.482512525152334 2314 44-45 -2.4543039642392657 2314 46-47 -2.4693734976330575 2314 48-49 -1.5155650072531586 2314 50-51 -2.0772064586520997 2314 52-53 -0.6436316463289806 2314 54-55 -0.49373284446691557 2314 56-57 -0.9948898440524765 2314 58-59 -1.5589481690939948 2314 60-61 -0.5706907417932143 2314 62-63 -3.0209108741733672 2314 64-65 -1.1179282554192937 2314 66-67 -2.4232755582407783 2314 68-69 -2.2440554991571204 2314 70-71 -2.6959698356590422 2314 72-73 -2.4673331679666575 2314 74-75 -1.8653937405049987 2314 76-77 -0.5063159542134699 2314 78-79 -0.18002299162167645 2314 80-81 -0.23334020254704768 2314 82-83 -0.127243714099869 2314 84-85 0.15680816476199766 2314 86-87 0.5730259185812336 2314 88-89 0.7268611085307661 2314 90-91 1.1219384177991216 2314 92-93 1.438208203668779 2314 94-95 1.4029882563741012 2314 96-97 1.3525866448362436 2314 98-99 1.700141878850781 2314 100-101 1.667921867159171 2315 1 -14.302190431776221 2315 2 -7.390972588175192 2315 3 -4.7523444419912195 2315 4 -3.465484659536642 2315 5 -3.7356229984402702 2315 6 -3.3996203003928223 2315 7 -3.1380083698783814 2315 8 -3.436713864061236 2315 9 -2.2245524473158014 2315 10-11 -4.4037647621256575 2315 12-13 -4.821352098743709 2315 14-15 -3.530810582865577 2315 16-17 -4.8620253741472865 2315 18-19 -1.3943138739187262 2315 20-21 -3.8375750284471195 2315 22-23 -4.579818507495588 2315 24-25 -5.1330344342749825 2315 26-27 -4.836191228798143 2315 28-29 -3.1128954085315357 2315 30-31 -3.6367631116382952 2315 32-33 -4.9862414703906595 2315 34-35 -4.477086011770929 2315 36-37 -5.4257895960008575 2315 38-39 -4.385180551387716 2315 40-41 -5.824853267587599 2315 42-43 -4.194602699905516 2315 44-45 -3.0636580110160985 2315 46-47 -2.431977108846823 2315 48-49 -2.178732210674305 2315 50-51 -2.0154462559172934 2315 52-53 -2.097791933536623 2315 54-55 -3.1904793203657604 2315 56-57 -5.0468406386250955 2315 58-59 -4.53903501772923 2315 60-61 -5.714660173185429 2315 62-63 -3.7657144748280302 2315 64-65 -4.103248460265924 2315 66-67 -2.894880806115758 2315 68-69 -2.2398292175458003 2315 70-71 -2.693142635405625 2315 72-73 -2.8713443078219996 2315 74-75 -3.450184670234158 2315 76-77 -3.178891319822469 2315 78-79 -2.7410862934536837 2315 80-81 -2.239248021563995 2315 82-83 -2.372470998826124 2315 84-85 -2.3842113167859686 2315 86-87 -2.9213962344201896 2315 88-89 -3.383134403849784 2315 90-91 -3.011580139827192 2315 92-93 -1.8143734974029684 2315 94-95 -1.6013620947161264 2315 96-97 -2.6959152822003425 2315 98-99 -2.3017548003108264 2315 100-101 -1.828165984591001 2316 1 -14.302190431776221 2316 2 -7.391401745268013 2316 3 -5.213005930340007 2316 4 -2.7570315975940822 2316 5 -2.1669178800089064 2316 6 -0.9981558351332254 2316 7 -4.424610671866365 2316 8 -2.7495560568673874 2316 9 -1.1209549913377188 2316 10-11 -4.702388793856628 2316 12-13 -3.6960328964960993 2316 14-15 -1.6902890903584975 2316 16-17 -3.0644955597244135 2316 18-19 -2.2934299702418386 2316 20-21 -3.2096315705604894 2316 22-23 -2.9176304460594764 2316 24-25 -3.276162323088645 2316 26-27 -4.3673570966817294 2316 28-29 -4.018688762727415 2316 30-31 -2.9655560873229483 2316 32-33 -2.9757870969866715 2316 34-35 -4.013369777904305 2316 36-37 -2.4359507564842886 2316 38-39 -3.1832408146396105 2316 40-41 -2.7460136443928604 2316 42-43 -6.972835105797497 2316 44-45 -3.7923227726873066 2316 46-47 -4.773826056212442 2316 48-49 -4.9012275059397155 2316 50-51 -3.521033294839974 2316 52-53 -4.917509969329924 2316 54-55 -4.822410473972013 2316 56-57 -5.03505481200456 2316 58-59 -3.8494835268748098 2316 60-61 -3.5847681821630744 2316 62-63 -2.2213180402971453 2316 64-65 -2.5693730384391564 2316 66-67 -2.8816369116084353 2316 68-69 -2.9556925696625314 2316 70-71 -3.163149619204276 2316 72-73 -3.755987680943438 2316 74-75 -4.395857647198284 2316 76-77 -3.668578221734748 2316 78-79 -3.5769390365938314 2316 80-81 -3.4970314918188734 2316 82-83 -3.1764661581473597 2316 84-85 -2.9914686031270428 2316 86-87 -2.7617644565369766 2316 88-89 -2.94301647894428 2316 90-91 -2.1501352398661098 2316 92-93 -1.8126901886817492 2316 94-95 -0.7931659939148545 2316 96-97 -1.0883847747287447 2316 98-99 -0.845904669431242 2316 100-101 -0.8026098129921397 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3954.0 3 1075.0 4 931.0 5 1048.0 6 1492.0 7 2159.0 8 2790.0 9 3753.0 10 4621.0 11 5812.0 12 7154.0 13 8200.0 14 8928.0 15 9899.0 16 11173.0 17 11652.0 18 9438.0 19 8908.0 20 9535.0 21 10171.0 22 11443.0 23 12606.0 24 14372.0 25 15824.0 26 17808.0 27 20164.0 28 22606.0 29 25198.0 30 28899.0 31 33187.0 32 37972.0 33 44731.0 34 54020.0 35 53136.0 36 46450.0 37 25582.0 38 3544.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.80059907402741 17.37782706057704 14.072839337274356 15.748734528121197 2 12.927955884298026 24.77218079027067 42.27551286877546 20.02435045665585 3 22.876163983920485 34.10706107841308 30.445918211577926 12.570856726088506 4 10.207306743707214 16.404612248292814 41.477813782087736 31.910267225912243 5 10.048665803833895 44.449570930112245 31.962186890935335 13.539576375118525 6 21.67139747437644 38.776448551829915 21.323076557646008 18.229077416147636 7 23.173732149644348 41.023748438942285 20.67332491719607 15.129194494217298 8 22.119853199427865 30.68966745903684 22.17771149242158 25.012767849113715 9 28.649129176407516 20.78966208719433 22.46768978118288 28.09351895521527 10-11 21.789993825443247 27.142011412752154 27.536233113265794 23.5317616485388 12-13 32.48873315062079 25.46770254669391 26.393819328340545 15.64974497434475 14-15 27.107322758899166 25.808140018221643 32.26095970709643 14.823577515782759 16-17 16.485405054174898 30.438378544823124 34.0681349125898 19.00808148841218 18-19 15.574020817957395 32.17154879155745 37.155812316018974 15.098618074466188 20-21 18.48529205860265 29.68895451209008 33.24372372066579 18.582029708641475 22-23 16.973421554715138 29.116705481358764 31.790930262559325 22.118942701366777 24-25 18.475319039113277 29.253968038359403 35.02460285658012 17.2461100659472 26-27 21.545301049153775 25.809318699453392 36.49313198590489 16.15224826548794 28-29 17.153686176308305 26.754636899961802 39.65188234794788 16.439794575782013 30-31 15.918881608104368 33.35920002309324 36.32370945311478 14.39820891568761 32-33 15.679220071411956 32.20354954437949 33.94385519298716 18.17337519122139 34-35 17.61601692210535 28.155154383653734 34.23365000033953 19.995178693901387 36-37 20.072105897899203 28.428021832063532 36.108454195865626 15.391418074171645 38-39 22.465064490449915 25.074810279772624 37.39334337027312 15.066781859504344 40-41 17.03906442277499 28.154305742970863 40.09488165253626 14.711748181717885 42-43 15.044925169975661 32.5219534217467 36.297398854722154 16.13572255355549 44-45 15.636049750720172 33.7474134140897 32.44287443324274 18.17366240194739 46-47 15.987968592014548 30.619559782225743 29.903634975978783 23.48883664978093 48-49 18.408282054416777 30.027795889536385 31.577200239075214 19.986721816971624 50-51 22.22250524728466 31.093218266663953 31.609710703102177 15.074565782949211 52-53 16.9885419258839 31.21503486852219 35.61600206552785 16.18042114006606 54-55 14.190230009018295 30.7875469384497 36.78055308821456 18.241669964317435 56-57 17.628830181118293 34.782641917597246 30.194362774854845 17.39416512642962 58-59 15.985196676125948 31.423627612051757 31.95537278170783 20.635802930114462 60-61 16.639451117252055 28.536352278976924 33.86018902641889 20.964007577352138 62-63 23.473279041245295 27.631843625206354 31.596806980168818 17.298070353379533 64-65 21.070623250161457 28.89224771136607 34.529920913026395 15.507208125446079 66-67 16.252541952830864 30.096297151315365 35.9552650251055 17.695895870748277 68-69 15.45630599972504 34.54793720732479 33.70930976599561 16.286447026954548 70-71 16.38865760368155 33.331126575391764 30.149745501396623 20.130470319530062 72-73 16.001972900630836 31.458426891892007 28.956816057577456 23.582784149899698 74-75 17.53868859586552 31.86391396797576 31.267535546633514 19.32986188952521 76-77 20.306405715406648 28.491859214038172 32.993090118878484 18.20864495167669 78-79 17.039054201045964 28.674646824113015 35.62964529142689 18.656653683414127 80-81 15.918107401243164 34.58459291464285 32.57353690431711 16.923762779796885 82-83 16.02866507038905 33.10187307894781 30.397541053203593 20.471920797459543 84-85 17.384377055790164 29.386159624811665 30.670967468115357 22.558495851282814 86-87 19.276810795713565 29.808421247439405 32.45318770167469 18.46158025517234 88-89 21.562160418364577 26.48167957076881 33.58572738386308 18.370432627003535 90-91 17.252663021749814 28.755881396748435 35.64998612147402 18.341469460027724 92-93 15.644578747893846 32.232375148014384 33.52081114001842 18.602234964073354 94-95 15.88376654806688 33.37516877117601 30.751254638553704 19.98981004220341 96-97 16.049624950416668 30.650524797610828 28.699468534652272 24.600381717320232 98-99 18.048261226376145 29.743164097344817 29.856494043806187 22.352080632472855 100-101 20.837686995081427 30.06965858796945 30.701937698038517 18.390716718910607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8719.0 1 6557.5 2 4138.0 3 3253.5 4 2698.5 5 3207.5 6 5324.5 7 11462.0 8 13984.5 9 10022.0 10 6794.0 11 5166.0 12 4708.0 13 4228.5 14 3474.5 15 2959.5 16 2635.5 17 2461.5 18 2359.5 19 2352.5 20 2417.0 21 2582.5 22 2783.0 23 2952.0 24 3274.5 25 3805.5 26 4329.5 27 4798.5 28 5346.5 29 6276.5 30 7305.0 31 8047.0 32 8945.5 33 9943.5 34 10913.5 35 12182.0 36 14002.5 37 14961.0 38 14506.0 39 13415.0 40 12008.5 41 10896.0 42 10363.0 43 11456.5 44 13675.5 45 16251.5 46 23349.0 47 46664.0 48 66312.5 49 48149.5 50 21166.0 51 11302.0 52 7023.5 53 4920.0 54 3768.5 55 3186.5 56 2955.0 57 2928.5 58 2974.0 59 3158.5 60 3154.0 61 2750.0 62 2054.5 63 1291.5 64 802.5 65 555.0 66 415.5 67 323.0 68 259.5 69 216.0 70 194.0 71 163.5 72 144.0 73 129.0 74 101.0 75 85.5 76 58.0 77 32.5 78 24.5 79 16.5 80 14.5 81 10.5 82 5.5 83 4.0 84 3.0 85 2.0 86 1.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 1.0 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 52.942751040212514 2 0.09182331973489828 3 0.11757450903324614 4 0.1926256725804047 5 0.12418172207690119 6 0.14654459699388747 7 0.15586246154263178 8 0.15128823712779366 9 0.13925972107396012 10-11 0.12672295786292234 12-13 0.13781968746188145 14-15 0.1448504398032067 16-17 0.1406997546860388 18-19 0.1472222598701598 20-21 0.17644647140940325 22-23 0.19313391973760893 24-25 0.22159576054104602 26-27 0.21439559248065274 28-29 0.20990607592534868 30-31 0.2282029735847011 32-33 0.2180380304406164 34-35 0.20533185151051053 36-37 0.1915244704064622 38-39 0.18754320100836236 40-41 0.1878820324464985 42-43 0.17991949365029886 44-45 0.19770814415244706 46-47 0.22117222124337582 48-49 0.2249840749224076 50-51 0.23514901806649227 52-53 0.2630179038531911 54-55 0.247516365558462 56-57 0.2269323556916905 58-59 0.18161365084097963 60-61 0.19313391973760893 62-63 0.197623436292913 64-65 0.186780830272556 66-67 0.1948280769282897 68-69 0.1841548866270008 70-71 0.19728460485477686 72-73 0.21744507542387814 74-75 0.24734694983939387 76-77 0.24895639917054063 78-79 0.24201035468874943 80-81 0.24463629833430464 82-83 0.23616551238090075 84-85 0.20778837943699768 86-87 0.2184615697382866 88-89 0.22091809766477374 90-91 0.20744954799886153 92-93 0.2060942222463169 94-95 0.24548337692964503 96-97 0.2725051841210035 98-99 0.29088678963988995 100-101 0.28444899231530296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 590264.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.22470342972003 #Duplication Level Percentage of deduplicated Percentage of total 1 89.25155634611565 52.85896955239645 2 6.230418695362401 7.379893989516427 3 1.922960954557186 3.41660376721742 4 0.7837312902636416 1.8566501293782396 5 0.4459468569281382 1.3205535173492386 6 0.29241376937638214 1.039087126204967 7 0.19160100232133767 0.7943258777522826 8 0.12548479175637967 0.594543966136941 9 0.09804898362230464 0.5226229778954822 >10 0.5669217042267373 6.0976547627738595 >50 0.047562330639365887 1.901757745538578 >100 0.029463390661500787 3.608578466952107 >500 0.004629961389664409 1.9296771147192773 >1k 0.00799720603669307 10.994248736284588 >5k 0.0012627167426357481 5.684832269884138 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9514 1.6118211512136942 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7464 1.2645189271241344 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5827 0.9871853950096906 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3818 0.6468292154019218 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.6300570592141821 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3462 0.5865172194136861 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3359 0.5690674003496741 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3252 0.5509399184093897 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3205 0.54297737961319 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3195 0.5412832224225093 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2792 0.47300868763807385 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2623 0.44437743111556866 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2246 0.3805077050269032 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1776 0.30088231706490653 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1542 0.2612390388029763 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1466 0.24836344415380238 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1383 0.2343019394711519 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1347 0.2282029735847011 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1340 0.22701706355122453 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1228 0.2080425030155998 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1177 0.19940230134312784 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1174 0.19889405418592357 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 966 0.16365558461976337 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 951 0.1611143488337422 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 830 0.14061504682650475 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 818 0.13858205819768782 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 808 0.13688790100700704 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.10012468996923411 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.470785953403901E-5 0.0 12-13 0.0 0.0 0.0 2.5412357860211704E-4 0.0 14-15 0.0 0.0 0.0 8.470785953403901E-4 0.0 16-17 0.0 0.0 0.0 0.001355325752544624 0.0 18-19 0.0 0.0 0.0 0.002032988628816936 0.0 20-21 0.0 0.0 0.0 0.0035577301004296384 0.0 22-23 0.0 0.0 0.0 0.006691920903189081 0.0 24-25 0.0 0.0 0.0 0.01279088678963989 0.0 26-27 0.0 0.0 0.0 0.0169415719068078 0.0 28-29 0.0 0.0 0.0 0.027106515050892482 0.0 30-31 0.0 0.0 0.0 0.053111827927842456 0.0 32-33 0.0 0.0 0.0 0.09470338695905561 0.0 34-35 0.0 0.0 0.0 0.17864887575728827 0.0 36-37 0.0 0.0 0.0 0.29656221622867057 0.0 38-39 0.0 0.0 0.0 0.4216757247604462 0.0 40-41 0.0 0.0 0.0 0.5611048615534744 0.0 42-43 0.0 0.0 0.0 0.6902843473428839 0.0 44-45 0.0 0.0 0.0 0.825393383299676 0.0 46-47 0.0 0.0 0.0 1.0001456975183984 0.0 48-49 0.0 0.0 0.0 1.2284333789626336 0.0 50-51 0.0 0.0 0.0 1.4432525107409566 0.0 52-53 0.0 0.0 0.0 1.6299486331539785 0.0 54-55 0.0 0.0 0.0 1.7996184758006586 0.0 56-57 0.0 0.0 0.0 1.9814015423607063 0.0 58-59 0.0 0.0 0.0 2.2728812870173347 0.0 60-61 0.0 0.0 0.0 2.7324214249894965 0.0 62-63 0.0 0.0 0.0 3.1084396134610954 0.0 64-65 0.0 0.0 0.0 3.359598416979521 0.0 66-67 0.0 0.0 0.0 3.641675589227871 0.0 68-69 0.0 0.0 0.0 3.9517910629819877 0.0 70-71 0.0 0.0 0.0 4.3349247116544465 0.0 72-73 0.0 0.0 0.0 4.793871894609869 0.0 74-75 0.0 0.0 0.0 5.220799506661426 0.0 76-77 0.0 0.0 0.0 5.5617486412859325 0.0 78-79 0.0 0.0 0.0 5.951404795142512 0.0 80-81 0.0 0.0 0.0 6.399848203515715 0.0 82-83 0.0 0.0 0.0 6.8582024314544 0.0 84-85 0.0 0.0 0.0 7.579913394684413 0.0 86-87 0.0 0.0 0.0 8.379979127983411 0.0 88-89 0.0 0.0 0.0 8.902287789870297 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTATC 15 0.009031446 133.71074 1 CGTCACT 40 0.001671974 75.21229 1 CCCTAAG 50 0.0040496057 60.16983 1 GAACTCG 50 0.0016703168 37.741207 9 GGTCGGG 240 2.222805E-9 37.606144 1 GTCGGGA 215 0.0 35.14388 2 GCTTTTA 485 0.0 33.08307 1 GTACATG 6540 0.0 31.894302 1 CGTTGGA 60 0.004063429 31.483059 2 GTCACTA 90 2.5087249E-5 31.483057 2 GACGTGA 75 3.1976707E-4 31.459013 7 GATGACC 60 0.004083741 31.451008 9 TACCTGG 200 0.0 30.69598 2 GGATATG 135 0.005387511 29.713495 1 CGGGAGT 255 0.0 29.631113 4 GGATGAC 80 4.672046E-4 29.48782 8 CCCTAAT 140 0.006435208 28.6523 1 TACGTGG 70 0.008629618 26.98548 2 ATGGGCG 70 0.008661839 26.964869 5 GGGAATG 320 0.0 26.543543 7 >>END_MODULE