FastQCFastQC Report
Fri 27 May 2016
SRR939655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511181
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102840.6805273491395141No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA91060.6025750720793869No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA71400.4724781478856603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67110.4440897549664799No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66280.4385973619308342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51400.340131327749621No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34810.2303496404467764No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33580.22221031100841No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA30910.20454201052024873No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29660.19627033426174628No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT29190.19316018398854937No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26840.17760943262256473No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24340.1610660801055598No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG24070.1592793980337233No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22990.15213266974637718No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19280.12758233461114188No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG16850.11150219596461311No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16650.11017872776325272No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15860.10495102836787916No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15500.10256878560543047No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15240.10084827694366194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95650.029.3262271
TACATGG95900.028.7548562
GTTACCG700.0084272127.1198259
ACATGGG98450.026.7566013
CATGGGA75450.025.1608724
TTTGCGT3450.023.3874873
ATGGGAT29200.022.5921135
GAGTACT70800.021.95491412-13
AGTACTT73750.021.72028412-13
ATGGGAG25700.020.8674935
TATCACG1150.003740733720.6360172
TACCTGG8750.020.6124322
CTTTGCG4000.020.1717072
GTACTTT79600.019.6181514-15
CATGGGG26400.018.8760154
TACTTTT88900.018.20656214-15
TTGCGTT4450.018.130674
GTGTAGC7250.017.6758161
ACTTTTT93200.017.67271416-17
GCTTATA11250.017.297571
Produced by FastQC (version 0.11.5)