##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939655_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1511181 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.163519790150882 31.0 30.0 33.0 26.0 34.0 2 30.268314649271 31.0 30.0 34.0 26.0 34.0 3 30.291670554354507 31.0 30.0 34.0 26.0 34.0 4 33.86800323720322 35.0 35.0 37.0 28.0 37.0 5 33.87678643392155 35.0 35.0 37.0 28.0 37.0 6 34.01542436015276 36.0 35.0 37.0 29.0 37.0 7 33.83419921240407 35.0 35.0 37.0 28.0 37.0 8 33.84524487801263 35.0 35.0 37.0 28.0 37.0 9 35.06461039412221 37.0 34.0 39.0 28.0 39.0 10-11 34.95190814336601 37.0 34.0 39.0 27.5 39.0 12-13 34.78712940408859 37.0 34.0 39.0 27.0 39.0 14-15 35.362067482320114 38.0 34.0 40.0 27.0 41.0 16-17 35.13553704023542 38.0 33.0 40.0 26.5 41.0 18-19 35.20409699433754 38.0 33.5 40.0 27.0 41.0 20-21 35.36705331790169 38.0 34.0 40.0 27.0 41.0 22-23 35.233238771530345 38.0 34.0 40.0 26.0 41.0 24-25 35.07824641786788 38.0 34.0 40.0 25.0 41.0 26-27 35.14482083880091 38.0 34.0 40.0 25.5 41.0 28-29 35.00943235787109 38.0 34.0 40.0 25.0 41.0 30-31 34.76094590919288 38.0 34.0 40.0 24.5 41.0 32-33 34.492267306166504 38.0 33.0 40.0 24.0 41.0 34-35 34.236347929202395 38.0 33.0 40.0 24.0 41.0 36-37 33.886858688668 38.0 33.0 40.0 22.5 41.0 38-39 33.67245981785107 37.5 32.5 40.0 21.0 41.0 40-41 33.36852336020635 37.0 32.0 40.0 18.5 41.0 42-43 33.02385749953183 37.0 31.0 40.0 17.0 41.0 44-45 32.721669012514056 37.0 31.0 40.0 15.5 41.0 46-47 32.15316034280474 37.0 30.5 40.0 10.5 41.0 48-49 31.720958640956972 36.5 30.0 39.0 8.5 41.0 50-51 31.07090116935033 35.5 29.5 38.5 7.5 40.0 52-53 31.0936869905061 36.0 29.5 39.0 6.5 40.0 54-55 31.133575660361004 36.0 29.0 39.0 2.0 40.0 56-57 31.012649047334502 36.0 29.0 39.0 2.0 40.0 58-59 30.62645043843193 35.5 28.0 39.0 2.0 40.0 60-61 30.166596522851997 35.0 28.0 39.0 2.0 40.0 62-63 29.74181319113991 35.0 27.5 38.0 2.0 40.0 64-65 29.09385275489832 34.0 26.0 38.0 2.0 40.0 66-67 28.535973850915276 34.0 26.0 37.5 2.0 39.5 68-69 28.040211265228983 33.5 25.5 37.0 2.0 39.0 70-71 27.45548183837674 33.0 24.5 36.0 2.0 39.0 72-73 26.689842249207736 32.0 22.0 35.5 2.0 38.5 74-75 26.224016183369166 32.0 22.0 35.0 2.0 38.0 76-77 25.835366180490624 32.0 20.5 35.0 2.0 37.0 78-79 25.249908184393533 31.0 18.5 35.0 2.0 36.5 80-81 24.578438651624126 31.0 16.0 34.0 2.0 36.0 82-83 23.92629274719574 30.5 9.0 34.0 2.0 35.5 84-85 23.27729338841608 30.0 4.5 34.0 2.0 35.0 86-87 22.589293406944634 29.0 2.0 34.0 2.0 35.0 88-89 21.75420614737745 29.0 2.0 34.0 2.0 35.0 90-91 20.49597268626326 27.5 2.0 33.0 2.0 35.0 92-93 18.935864400095024 25.0 2.0 32.0 2.0 34.0 94-95 18.66139595455475 25.0 2.0 32.0 2.0 34.0 96-97 17.99410461089704 24.5 2.0 32.0 2.0 34.0 98-99 17.003818536627975 20.5 2.0 32.0 2.0 34.0 100-101 15.398392383175807 2.0 2.0 30.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.1084313838522561 1101 2 0.9940630827471217 1101 3 1.1424485532108228 1101 4 1.226021093872312 1101 5 1.11557346433991 1101 6 1.0510333157323757 1101 7 0.9226583779111834 1101 8 1.040195498501177 1101 9 1.3016296837060821 1101 10-11 1.3418815705074039 1101 12-13 1.1216451871406434 1101 14-15 1.1520826196873415 1101 16-17 1.3141228353574093 1101 18-19 1.291821112157841 1101 20-21 1.3144241504847969 1101 22-23 1.3615301399993456 1101 24-25 1.1217596129484875 1101 26-27 1.14982885516293 1101 28-29 1.301915622092821 1101 30-31 1.4563940413380507 1101 32-33 1.193640721980472 1101 34-35 1.3445248736799442 1101 36-37 1.0781497272072897 1101 38-39 1.1025564259823781 1101 40-41 1.048171049574755 1101 42-43 1.1827151810303889 1101 44-45 0.8856420728150525 1101 46-47 0.9416908357114835 1101 48-49 0.6387984893073195 1101 50-51 0.7644899995582435 1101 52-53 0.9345344972976051 1101 54-55 1.0761059198180263 1101 56-57 0.8674869467868795 1101 58-59 0.85591374311797 1101 60-61 0.73658841555973 1101 62-63 0.7811385720712885 1101 64-65 0.8309934317935692 1101 66-67 0.7692090410217354 1101 68-69 0.5987002872993195 1101 70-71 0.5352157554422448 1101 72-73 0.7207370508873758 1101 74-75 0.5920252696441217 1101 76-77 0.00627291381556816 1101 78-79 0.24475261757782718 1101 80-81 0.17919753820782702 1101 82-83 0.10382046848484805 1101 84-85 -0.29046782538480187 1101 86-87 -0.8076092583799444 1101 88-89 -1.1552392537684497 1101 90-91 -4.608554910003171 1101 92-93 -9.140386867599425 1101 94-95 -7.697943750020482 1101 96-97 -4.743102646017581 1101 98-99 -3.429780609629283 1101 100-101 -2.8473431926487383 1102 1 0.8332636750028506 1102 2 0.6565424957326726 1102 3 0.7555505978528423 1102 4 0.8157470020441835 1102 5 0.728199497038112 1102 6 0.8436039141282663 1102 7 0.8286081924606847 1102 8 0.6725609631986273 1102 9 1.020367549083744 1102 10-11 0.7364419122183605 1102 12-13 0.8034178127716416 1102 14-15 0.9203712529773753 1102 16-17 0.6672289639274993 1102 18-19 0.8847003483963434 1102 20-21 0.8054994286605535 1102 22-23 0.8320977390513349 1102 24-25 0.7183140497011564 1102 26-27 0.6308941578422562 1102 28-29 1.1045735228198978 1102 30-31 0.9065999114825303 1102 32-33 1.0108350633968612 1102 34-35 0.7150094551777428 1102 36-37 0.7191450005626763 1102 38-39 0.7534773182871888 1102 40-41 0.8426152336594939 1102 42-43 0.9515196613003312 1102 44-45 0.5793551668257351 1102 46-47 0.632326589497481 1102 48-49 0.5540874439556305 1102 50-51 0.5889634406358688 1102 52-53 0.7795822323912631 1102 54-55 0.703610840616598 1102 56-57 0.5690354919711957 1102 58-59 0.3658249678946923 1102 60-61 0.46139526584694224 1102 62-63 0.34746758938446476 1102 64-65 0.3582154903611148 1102 66-67 0.26727453116005506 1102 68-69 -0.0585506935129132 1102 70-71 -0.10057016638735305 1102 72-73 0.15299070968523054 1102 74-75 0.07431374225502196 1102 76-77 -0.17399836612765895 1102 78-79 -0.28804835453095023 1102 80-81 -0.6634363275983155 1102 82-83 -0.5647691034391542 1102 84-85 -0.8360742552282723 1102 86-87 -0.8494611523852704 1102 88-89 -1.396695140650337 1102 90-91 -5.366544345349901 1102 92-93 -10.22573058705365 1102 94-95 -8.877011944924273 1102 96-97 -5.715455791981187 1102 98-99 -4.439279089872445 1102 100-101 -3.557840896276071 1103 1 0.9298696827208133 1103 2 0.9912263410031095 1103 3 0.8398187999696702 1103 4 0.9078612073876613 1103 5 0.9206388298816037 1103 6 0.7174123873389817 1103 7 0.7291586734051165 1103 8 0.7232758300253792 1103 9 0.8368591268927332 1103 10-11 0.7732110647987938 1103 12-13 0.8462160071862712 1103 14-15 0.8850494644117006 1103 16-17 0.8995328581689677 1103 18-19 0.9050609533048757 1103 20-21 0.9104416916897833 1103 22-23 1.0030511771687713 1103 24-25 0.8715753218056008 1103 26-27 0.8677043822698565 1103 28-29 1.01701464824761 1103 30-31 1.0071853084217395 1103 32-33 1.1204247487490804 1103 34-35 1.0409977895912235 1103 36-37 1.2057257348130506 1103 38-39 1.051689215530736 1103 40-41 1.1112874113735316 1103 42-43 1.1802827092450627 1103 44-45 1.0121660078235166 1103 46-47 1.000910960132984 1103 48-49 1.04166321018689 1103 50-51 0.9385710419808966 1103 52-53 1.175223297934842 1103 54-55 1.1818068114388467 1103 56-57 1.014433081240906 1103 58-59 0.8979378337246935 1103 60-61 0.6849654829466196 1103 62-63 0.8909175654505113 1103 64-65 0.894781007344374 1103 66-67 0.7630873203620006 1103 68-69 0.8214207728800709 1103 70-71 1.132886349656271 1103 72-73 0.8748821027201537 1103 74-75 0.5292849131670927 1103 76-77 0.5422178234686541 1103 78-79 0.4706981267838195 1103 80-81 0.13862322362527024 1103 82-83 0.18420680895666663 1103 84-85 -0.07375885692984951 1103 86-87 -0.34751664173121455 1103 88-89 -0.6062152402471028 1103 90-91 -2.514072795237322 1103 92-93 -8.763013137723703 1103 94-95 -5.5797024956416035 1103 96-97 -3.1453169947461 1103 98-99 -2.218503875993509 1103 100-101 -1.9746374103892244 1104 1 0.8139724740550669 1104 2 0.8125399514752196 1104 3 0.7467243550996088 1104 4 0.5992938134680941 1104 5 0.584988391645652 1104 6 0.6656763186457795 1104 7 0.790516676879534 1104 8 0.7173316401459076 1104 9 0.7140010789010773 1104 10-11 0.6892658437758925 1104 12-13 0.6385851316875204 1104 14-15 0.6851264895836024 1104 16-17 0.7703570896254632 1104 18-19 0.6558230262758755 1104 20-21 0.759601558262311 1104 22-23 0.6731457400612726 1104 24-25 0.6212008593771117 1104 26-27 0.7353097069454577 1104 28-29 0.5370030944718209 1104 30-31 0.5882387725874381 1104 32-33 0.6582478351808518 1104 34-35 0.4999934807368476 1104 36-37 0.5634476118253815 1104 38-39 0.40445787705272807 1104 40-41 0.3510731902905846 1104 42-43 0.5046754630813126 1104 44-45 0.398777707781484 1104 46-47 0.1286340173697269 1104 48-49 0.13516307249404136 1104 50-51 0.1269704713320401 1104 52-53 0.3650857914384318 1104 54-55 0.17697431012921072 1104 56-57 0.40072363592566873 1104 58-59 0.3094133419976508 1104 60-61 0.3310501402363677 1104 62-63 0.2013043684710567 1104 64-65 -0.14282818929551055 1104 66-67 -0.0706200957954799 1104 68-69 -0.07298765516454253 1104 70-71 0.28200749658592983 1104 72-73 0.01583775319980063 1104 74-75 -0.44269345352655876 1104 76-77 -0.32278790165500837 1104 78-79 -0.3741887062599787 1104 80-81 -0.18326041453034847 1104 82-83 -0.2958952286904193 1104 84-85 -0.5714406477236835 1104 86-87 -0.6709215270320392 1104 88-89 -0.6534743243515564 1104 90-91 -1.1514072273526814 1104 92-93 -4.4710535717978175 1104 94-95 -1.912416452726987 1104 96-97 -0.9496007952343426 1104 98-99 -0.6818572869455757 1104 100-101 -0.5081094028357871 1105 1 1.1428965048840922 1105 2 1.0687275770475004 1105 3 1.055984590269727 1105 4 0.9424449777907427 1105 5 0.7921056638040085 1105 6 0.7573988409605903 1105 7 0.8095015588004202 1105 8 0.6849755747989192 1105 9 1.0380817251248544 1105 10-11 0.8808765861756811 1105 12-13 0.6389912859643871 1105 14-15 0.9676433286205324 1105 16-17 1.149929979187732 1105 18-19 1.0490974984605757 1105 20-21 0.9617366987385765 1105 22-23 0.9640188997068719 1105 24-25 1.1721984385082251 1105 26-27 1.0761515764490923 1105 28-29 1.1222318830492668 1105 30-31 1.1181898199837619 1105 32-33 0.9741249060941897 1105 34-35 1.0519939912602112 1105 36-37 0.9207419203132474 1105 38-39 0.9074104724876619 1105 40-41 0.781006328197762 1105 42-43 0.8811985930836173 1105 44-45 0.8288959104098552 1105 46-47 0.8722493874168293 1105 48-49 0.7928038369616175 1105 50-51 0.7562434284151429 1105 52-53 0.5728982399743785 1105 54-55 0.9876712156988532 1105 56-57 0.7761013820473401 1105 58-59 0.7662201830107911 1105 60-61 0.7041519402429586 1105 62-63 0.878596165513315 1105 64-65 0.7444990973551278 1105 66-67 0.5626635575756218 1105 68-69 0.5874194048795935 1105 70-71 0.7036563635534208 1105 72-73 0.9460325343334937 1105 74-75 0.8032476798353194 1105 76-77 0.36124589169830656 1105 78-79 0.11023271431246684 1105 80-81 0.1795753369271118 1105 82-83 0.3240487372527312 1105 84-85 0.1521564422931121 1105 86-87 0.012673792621466617 1105 88-89 -0.2877886997015082 1105 90-91 -1.1474741388509848 1105 92-93 -7.091306527892808 1105 94-95 -2.9315439929924594 1105 96-97 -0.9669207122331223 1105 98-99 -0.5737883415880454 1105 100-101 -0.5293601439180957 1106 1 0.9196294369005287 1106 2 1.0451613237132058 1106 3 0.9747611745102276 1106 4 1.0337226434240776 1106 5 0.8562746134111023 1106 6 0.914019668586576 1106 7 0.7897481163665674 1106 8 0.7948333716412534 1106 9 0.9258556779761236 1106 10-11 0.6992057441311275 1106 12-13 0.7796070490672662 1106 14-15 0.768151132672152 1106 16-17 0.8020468056602326 1106 18-19 0.7132930323081794 1106 20-21 0.8861671651793515 1106 22-23 0.7952675479658282 1106 24-25 0.8730842794713922 1106 26-27 0.8199083351543024 1106 28-29 0.6543046427637833 1106 30-31 0.8272298134954141 1106 32-33 0.8820005999879257 1106 34-35 0.9247996649679564 1106 36-37 0.958353664239425 1106 38-39 0.7075578122612924 1106 40-41 0.769475075944861 1106 42-43 0.7140959148340329 1106 44-45 0.8943864121761322 1106 46-47 1.0607906785998722 1106 48-49 0.8768194991344984 1106 50-51 0.8458661124321587 1106 52-53 0.9778541732164001 1106 54-55 1.09573254869575 1106 56-57 1.1780041828547745 1106 58-59 1.236641385519622 1106 60-61 1.1230506317576285 1106 62-63 0.9364403187827257 1106 64-65 0.9603086100074272 1106 66-67 0.88799158006859 1106 68-69 0.6614925611048115 1106 70-71 0.5985147912082418 1106 72-73 0.68901744890346 1106 74-75 0.9114531985434162 1106 76-77 0.6868280415072583 1106 78-79 0.6587309300142898 1106 80-81 0.8654672156669285 1106 82-83 0.6877873826279739 1106 84-85 0.4694717944667133 1106 86-87 0.4036288427260004 1106 88-89 0.4064208922529282 1106 90-91 0.09302852858467503 1106 92-93 -3.246518841352543 1106 94-95 -1.2302810162784539 1106 96-97 -0.2948062287784303 1106 98-99 -0.3143959942446699 1106 100-101 -0.016902047890413385 1107 1 0.5984393558482068 1107 2 0.5270381819833005 1107 3 0.5492338527399525 1107 4 0.6906063983666968 1107 5 0.5719219591774234 1107 6 0.4796878138414442 1107 7 0.35668087052536634 1107 8 0.38222888157902446 1107 9 0.4741696057515412 1107 10-11 0.42886625268957346 1107 12-13 0.5768505694792196 1107 14-15 0.8096669118209903 1107 16-17 0.7875716752802759 1107 18-19 0.6453667194225616 1107 20-21 0.6281368110625891 1107 22-23 0.5121503833521075 1107 24-25 0.5947453047329176 1107 26-27 0.503934984772691 1107 28-29 0.3928962327642651 1107 30-31 0.5958979018253601 1107 32-33 0.5193952341577628 1107 34-35 0.4867690043324089 1107 36-37 0.4948495415618481 1107 38-39 0.773026939252027 1107 40-41 0.7552589539328736 1107 42-43 0.551559296787083 1107 44-45 0.465759528727709 1107 46-47 0.4905186212896169 1107 48-49 0.5855714860330039 1107 50-51 0.6947797223038101 1107 52-53 0.6590361096268893 1107 54-55 0.8910800315197562 1107 56-57 0.700190887821698 1107 58-59 0.7667200732689423 1107 60-61 0.6844421832243199 1107 62-63 0.3845074989413284 1107 64-65 0.4972073405817987 1107 66-67 0.5844110028499436 1107 68-69 0.5872186821972427 1107 70-71 0.3852409954986271 1107 72-73 0.5277016513634933 1107 74-75 0.4460922288931535 1107 76-77 0.182348926383181 1107 78-79 0.3240770116095888 1107 80-81 0.3905619080753375 1107 82-83 0.23443100200170974 1107 84-85 -0.03479626058405927 1107 86-87 -0.24194787235603954 1107 88-89 -0.18530857427303005 1107 90-91 -0.3946196404035085 1107 92-93 -3.973763325109239 1107 94-95 -1.8181796548971896 1107 96-97 -0.7791766173128778 1107 98-99 -0.5528238156405543 1107 100-101 -0.22766063807918435 1108 1 0.3521050993391981 1108 2 0.33040099156692904 1108 3 0.19970749822350697 1108 4 -0.22611283552360817 1108 5 -0.9145573905898274 1108 6 -0.11484825756865291 1108 7 -0.004867871837589632 1108 8 0.12662439196494546 1108 9 0.4050534222786766 1108 10-11 0.3511432640711192 1108 12-13 0.2067853578115404 1108 14-15 0.22370328705290632 1108 16-17 0.2967235838758455 1108 18-19 0.3084061189749008 1108 20-21 0.29245828466406465 1108 22-23 0.24464800721065671 1108 24-25 0.1284118126041065 1108 26-27 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0.41850690717852856 2311 66-67 0.18403662263931508 2311 68-69 0.5948646271052773 2311 70-71 0.6049257518488069 2311 72-73 0.6711662668528824 2311 74-75 0.6128232786144032 2311 76-77 0.6310889165838454 2311 78-79 0.8627397610623966 2311 80-81 0.9418431056379859 2311 82-83 0.9434134785863932 2311 84-85 1.017379174096149 2311 86-87 1.0996295187871006 2311 88-89 1.1224974139185733 2311 90-91 2.1132279666088856 2311 92-93 3.0797683155992637 2311 94-95 2.2077431951596544 2311 96-97 1.7966806831012292 2311 98-99 1.7119112332710174 2311 100-101 1.7234408418482463 2312 1 0.08062061963084233 2312 2 -0.16664931948517037 2312 3 -0.1428067251303311 2312 4 -0.2331178418797748 2312 5 0.06710481953612657 2312 6 0.1534537306531547 2312 7 0.1816855416571741 2312 8 -0.3447614587814556 2312 9 -0.09554400631813564 2312 10-11 -0.052169325111712794 2312 12-13 -0.03577964880901163 2312 14-15 -0.4502855824888101 2312 16-17 0.009804920088981817 2312 18-19 0.013464213122595936 2312 20-21 0.2408923387550317 2312 22-23 0.07931212161731338 2312 24-25 0.3164911108770312 2312 26-27 0.011597319189924349 2312 28-29 0.07769310659298867 2312 30-31 0.2411833406115349 2312 32-33 0.17996413139398015 2312 34-35 0.06640909782217363 2312 36-37 0.07644218679489256 2312 38-39 0.24937945101562775 2312 40-41 0.32334559494343296 2312 42-43 0.23810267043697309 2312 44-45 0.13616931658560105 2312 46-47 0.3556290147723331 2312 48-49 0.24401609051214734 2312 50-51 0.22883945237392922 2312 52-53 0.15649822124698431 2312 54-55 0.14944042509571176 2312 56-57 0.10472171785105289 2312 58-59 0.41435580927335636 2312 60-61 0.3140915443887735 2312 62-63 0.2890909141670903 2312 64-65 0.09815581342937563 2312 66-67 0.23143193958994956 2312 68-69 -0.16809922888007023 2312 70-71 0.1819941766233022 2312 72-73 0.32861540306365455 2312 74-75 0.3164180669091188 2312 76-77 0.49850082375776594 2312 78-79 0.5354088976690328 2312 80-81 0.5127443578219761 2312 82-83 0.738651616666715 2312 84-85 0.839376874496871 2312 86-87 0.8332864394473241 2312 88-89 0.9839460175148034 2312 90-91 1.4055692933589121 2312 92-93 2.503180863350142 2312 94-95 1.7075128753570112 2312 96-97 1.4826335143253964 2312 98-99 1.588980557732242 2312 100-101 1.7100997472174004 2313 1 -0.1359660124262625 2313 2 -0.8224245232756289 2313 3 -0.32624498351085407 2313 4 -0.145540481510686 2313 5 -0.9269776567068391 2313 6 -0.466946827603806 2313 7 -1.3710492951771158 2313 8 -1.1440769717467063 2313 9 -1.2632227063261396 2313 10-11 -2.2379803533534712 2313 12-13 -0.830592303818662 2313 14-15 -0.365265003430558 2313 16-17 -1.7270107039942957 2313 18-19 -1.2691743510547298 2313 20-21 -1.5847158324200592 2313 22-23 -1.2237022881681838 2313 24-25 -0.4696283810024582 2313 26-27 -1.035093230444268 2313 28-29 -2.5258560920238153 2313 30-31 -0.8757448238075867 2313 32-33 -0.44664463537372256 2313 34-35 -0.19842338064315612 2313 36-37 0.06393971997473358 2313 38-39 -0.3190584073400018 2313 40-41 -0.3665702220407354 2313 42-43 -0.18894239014457526 2313 44-45 -0.7007988503225633 2313 46-47 -1.0681520204920822 2313 48-49 -0.5374629348494295 2313 50-51 -0.37273006750730886 2313 52-53 -0.15892069370772433 2313 54-55 -0.1428959869477069 2313 56-57 -0.3979440480188252 2313 58-59 -0.15265618258391456 2313 60-61 -0.2814614233514412 2313 62-63 -0.21174984925818663 2313 64-65 -0.036353533818900274 2313 66-67 -0.13692022454470631 2313 68-69 -0.11977035508347811 2313 70-71 -0.3093539072574707 2313 72-73 -0.4576112954864797 2313 74-75 -0.24496993056628114 2313 76-77 -0.015038152000727223 2313 78-79 0.06772059706365852 2313 80-81 -0.20375729116952002 2313 82-83 -0.15499207671864923 2313 84-85 -0.07300185129051329 2313 86-87 0.11829119529879861 2313 88-89 0.20915025244497798 2313 90-91 0.7879747763667417 2313 92-93 2.1623601900638008 2313 94-95 1.5879488135931688 2313 96-97 1.0703696401287779 2313 98-99 0.8329059575348978 2313 100-101 0.6207046737461592 2314 1 -0.49859158258631453 2314 2 -0.30716609570282216 2314 3 -1.5896394038143988 2314 4 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64-65 -0.3301237424502439 2314 66-67 -0.1612439944834385 2314 68-69 -0.1534793867785318 2314 70-71 -0.23665573446861643 2314 72-73 0.13925518585005747 2314 74-75 0.31121946603120065 2314 76-77 0.08958646451884533 2314 78-79 0.11202763331099419 2314 80-81 0.10514863129771257 2314 82-83 0.259889752831608 2314 84-85 0.2895445487166839 2314 86-87 0.3784430580465461 2314 88-89 0.7383676374908141 2314 90-91 1.3712992030578803 2314 92-93 2.1657045892253173 2314 94-95 1.6042283562056028 2314 96-97 1.2637004544117936 2314 98-99 1.3046979919379282 2314 100-101 1.3218033395491506 2315 1 -7.2295957510570545 2315 2 -2.9111348449069077 2315 3 -1.3603130235921377 2315 4 -0.8204567452623479 2315 5 -1.3867972208579147 2315 6 -1.6887890250671944 2315 7 -3.2719865105089063 2315 8 -2.8549819538783012 2315 9 -3.1852526972167574 2315 10-11 -2.14961110803079 2315 12-13 -2.7062198001398627 2315 14-15 -4.8501882743996525 2315 16-17 -4.247361415226862 2315 18-19 -3.2082215446641484 2315 20-21 -4.275709455852194 2315 22-23 -5.714448381644466 2315 24-25 -3.4499035894671835 2315 26-27 -4.356335960456185 2315 28-29 -2.7526879538414875 2315 30-31 -2.5257816116991094 2315 32-33 -1.4279082118575062 2315 34-35 -2.307877868765921 2315 36-37 -2.9918470153626657 2315 38-39 -2.0109880198408767 2315 40-41 -3.5825046344855522 2315 42-43 -7.803268821368732 2315 44-45 -4.2971431849872666 2315 46-47 -2.665815989380036 2315 48-49 -5.523085701298875 2315 50-51 -6.557879496419087 2315 52-53 -6.574331141468917 2315 54-55 -4.263618956606457 2315 56-57 -3.477639058880012 2315 58-59 -3.7247833986164274 2315 60-61 -2.6915891298891736 2315 62-63 -3.0255060681636614 2315 64-65 -1.6954799089853196 2315 66-67 -2.2925195168157444 2315 68-69 -1.6974817978695462 2315 70-71 -1.3902327875041287 2315 72-73 -1.8582620908056562 2315 74-75 -2.3940246569344374 2315 76-77 -2.498719744040528 2315 78-79 -1.4971692144421915 2315 80-81 -1.3882229908401769 2315 82-83 -1.6360799472252765 2315 84-85 -1.6284401527410068 2315 86-87 -1.8965513174893722 2315 88-89 -1.737874307852131 2315 90-91 -0.7249661527902909 2315 92-93 0.06954906960973162 2315 94-95 -0.2124188901416808 2315 96-97 -0.35868841466765744 2315 98-99 -0.29685807441801515 2315 100-101 -0.249786006654654 2316 1 -4.455344853514937 2316 2 -3.0926454773288903 2316 3 -4.5130850478948155 2316 4 -4.395108084671353 2316 5 -3.940270018427647 2316 6 -4.60467034238285 2316 7 -3.0284936908458313 2316 8 -1.560311973209977 2316 9 -1.87212269169342 2316 10-11 -3.0958233076993906 2316 12-13 -3.9604313439123047 2316 14-15 -3.1365991415634156 2316 16-17 -2.893505436215534 2316 18-19 -2.9652767435042477 2316 20-21 -5.655477648340462 2316 22-23 -6.680462604234911 2316 24-25 -4.541927546937494 2316 26-27 -4.024970315606694 2316 28-29 -4.514204972620831 2316 30-31 -6.176581632411626 2316 32-33 -4.678227529725497 2316 34-35 -6.362196427175199 2316 36-37 -9.287414538146429 2316 38-39 -6.433566382171726 2316 40-41 -5.862641337053901 2316 42-43 -4.850687524765583 2316 44-45 -3.6637416729740124 2316 46-47 -5.970988938841508 2316 48-49 -3.159313607954495 2316 50-51 -4.397012332652171 2316 52-53 -5.346083592449311 2316 54-55 -6.9092581101552 2316 56-57 -5.135912843746073 2316 58-59 -6.1450833848080855 2316 60-61 -6.1032502732197464 2316 62-63 -6.381817342673465 2316 64-65 -5.39713345772876 2316 66-67 -6.820118936865452 2316 68-69 -5.485778366496998 2316 70-71 -6.411583933596365 2316 72-73 -8.23460408887249 2316 74-75 -7.9978849510520895 2316 76-77 -7.337261410707196 2316 78-79 -6.378080910106373 2316 80-81 -6.131724440715903 2316 82-83 -5.179791974289621 2316 84-85 -6.429850329487763 2316 86-87 -5.464660955710858 2316 88-89 -5.538464614397284 2316 90-91 -6.008895833817629 2316 92-93 -4.930122982309626 2316 94-95 -4.587168613974875 2316 96-97 -5.187322597213917 2316 98-99 -5.968689923354773 2316 100-101 -5.079977942583955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7771.0 3 3168.0 4 3277.0 5 3749.0 6 4391.0 7 5231.0 8 6452.0 9 8121.0 10 9886.0 11 11995.0 12 14541.0 13 16699.0 14 18110.0 15 20560.0 16 23504.0 17 26209.0 18 23071.0 19 20659.0 20 21834.0 21 23300.0 22 25735.0 23 28806.0 24 31829.0 25 36045.0 26 40263.0 27 45687.0 28 51322.0 29 59447.0 30 66893.0 31 76179.0 32 87193.0 33 100913.0 34 118255.0 35 139957.0 36 156071.0 37 132967.0 38 40404.0 39 687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.420855184984525 18.576011953885345 13.535152291492528 21.467980569637607 2 14.934624049367637 21.572308338406245 40.557664575899224 22.93540303632689 3 20.352110623595742 27.182492369324684 29.919665945991774 22.545731061087796 4 11.995024015484374 16.37610282111561 39.03186895514261 32.59700420825741 5 12.547823944296365 39.56305341974321 33.594863628896796 14.294259007063637 6 29.088987288287132 37.17166044348606 18.541126636316797 15.198225631910015 7 26.95127347105314 34.85393434066443 21.11878134662311 17.076010841659315 8 25.55572923358974 33.24692115208588 20.56360435496199 20.63374525936239 9 27.530119564288736 17.1453189915352 20.871197344987976 34.45336409918809 10-11 24.458586871497463 26.57754442809937 27.598441911804155 21.365426788599013 12-13 27.607065943262228 24.410925778187988 27.50215176723094 20.479856511318843 14-15 23.87080471175179 25.186450815700585 27.940779531494197 23.001964941053423 16-17 21.134251248126777 28.71633783459319 28.24252158866047 21.906889328619567 18-19 20.818939007802495 28.05056204521466 30.848285713244834 20.282213233738013 20-21 22.65185226894458 26.28603888199766 29.938641365821667 21.12346748323609 22-23 21.711165715868145 26.53353941513138 29.705094561997786 22.050200307002697 24-25 22.078812788565237 26.44918352707688 30.405560994842894 21.066442689514986 26-27 22.256619428033705 25.818569189631578 31.075244946173143 20.849566436161577 28-29 21.147184275321045 26.81842776404367 31.1104285473353 20.923959413299986 30-31 21.234994151736753 27.06629246617782 30.54837822524263 21.1503351568428 32-33 21.188160802621507 27.039074122546893 30.277359009446307 21.495406065385293 34-35 22.019339850190043 26.314349100663172 30.18646064705602 21.479850402090765 36-37 22.232828632403727 26.121048039969637 30.702818437217434 20.943304890409202 38-39 22.283764858830548 26.13613586087374 30.84461139513564 20.73548788516007 40-41 21.59867427593057 26.572856183984634 30.94761971968171 20.880849820403082 42-43 20.918835217522094 27.392849049777833 29.97483478286221 21.713480949837862 44-45 21.42448268117339 27.24436502935995 29.384199622491252 21.946952666975406 46-47 21.88636872678212 27.17789187276436 28.622850834352718 22.3128885661008 48-49 22.166612043280278 27.07313977544652 28.742925332537123 22.01732284873608 50-51 22.125176933475664 27.58004717342248 29.10332384716925 21.19145204593261 52-53 21.89887422218716 27.971134004217273 28.76540296455487 21.364588809040693 54-55 20.941500494663106 27.355250969025967 29.27172664767558 22.43152188863534 56-57 21.86304908362088 28.118926176097737 28.05703239880798 21.960992341473403 58-59 22.1684140428415 27.34809091767481 28.24497612094085 22.238518918542837 60-61 21.77483023329856 27.06225502392487 29.258744761723648 21.90416998105292 62-63 22.49430929538625 26.948281591071648 28.795246940635455 21.762162172906645 64-65 21.96394565912639 27.47756416732611 28.967920229033805 21.590569944513692 66-67 21.43583844978796 27.530164527158757 28.716272064969395 22.317724958083886 68-69 21.24000899757536 28.061502866734845 28.708394081874623 21.990094053815174 70-71 21.731049377950928 27.870634466531335 27.98506856935174 22.413247586165998 72-73 21.636510233656985 27.385624662183773 28.136964411111954 22.84090069304729 74-75 21.495043676893076 28.265028390974813 27.94986404415284 22.290063887979276 76-77 22.07374831413153 27.122851874599558 28.231075266714416 22.572324544554494 78-79 21.584834568084553 27.319334772256017 28.71409764168704 22.38173301797239 80-81 21.526353855710262 28.168100919419985 28.175094848954323 22.130450375915427 82-83 21.54112649389977 27.781630104185172 28.0703004637818 22.60694293813326 84-85 21.533421328820225 27.402596584878143 27.90220239470118 23.16177969160045 86-87 21.79152257878507 27.485687868780744 28.155238778543485 22.567550773890694 88-89 22.0450713319507 26.92443463616081 28.094158638970274 22.936335392918213 90-91 21.30575186096645 27.066437447707493 28.754859346832127 22.872951344493934 92-93 20.993580735087157 27.73292744624086 28.838195873562533 22.43529594510945 94-95 21.317312871336355 27.717796799774607 27.94484541635247 23.020044912536562 96-97 20.92459009872107 27.367150879472778 27.881110396541782 23.82714862526437 98-99 21.492301561040108 27.29787743371664 27.39313396987997 23.816687035363284 100-101 21.77155504712641 27.121699700745232 27.895285701259194 23.211459550869165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2746.0 1 1802.5 2 956.0 3 1014.0 4 891.5 5 920.5 6 1322.0 7 2406.0 8 3726.0 9 4116.0 10 3447.5 11 2809.5 12 2676.0 13 2661.5 14 2782.5 15 2922.0 16 3019.5 17 3154.5 18 3339.5 19 3589.5 20 3971.0 21 4555.5 22 5203.0 23 6019.5 24 6867.5 25 8062.0 26 9609.0 27 11437.5 28 13522.0 29 15454.0 30 17871.5 31 20512.5 32 23344.0 33 26233.0 34 29326.5 35 31889.5 36 34622.5 37 37877.0 38 39329.0 39 39671.0 40 40451.5 41 42348.5 42 44511.5 43 46423.5 44 48601.0 45 50465.0 46 53935.0 47 64047.0 48 87702.0 49 88090.0 50 64547.0 51 53432.5 52 47923.5 53 44695.5 54 42163.0 55 39131.5 56 35443.0 57 31406.0 58 28185.5 59 25223.5 60 21856.5 61 18521.0 62 15151.0 63 11604.5 64 8498.5 65 6257.0 66 4485.5 67 3267.0 68 2290.0 69 1572.0 70 1154.5 71 871.0 72 668.0 73 554.0 74 420.5 75 308.0 76 230.5 77 139.5 78 81.0 79 56.5 80 38.0 81 29.0 82 18.5 83 10.5 84 8.5 85 8.0 86 7.5 87 5.5 88 4.0 89 5.0 90 7.0 91 5.0 92 2.5 93 1.5 94 2.5 95 2.0 96 0.5 97 1.0 98 2.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0912531324837991 2 0.10111297058393402 3 0.09826751395100917 4 0.10124531740407007 5 0.09773812667046501 6 0.08172416143400427 7 0.09588527118856047 8 0.09019435792271077 9 0.0848343117072012 10-11 0.09145165271400316 12-13 0.09102152554856102 14-15 0.09704330586475081 16-17 0.09386698218148587 18-19 0.09737417291509091 20-21 0.10677079714474971 22-23 0.11567112079889834 24-25 0.13886490102773924 26-27 0.14399334030801075 28-29 0.1432323460922285 30-31 0.14465507440869094 32-33 0.14068466980460978 34-35 0.13691278543073265 36-37 0.14131331720025594 38-39 0.13363720163236567 40-41 0.13022927101386267 42-43 0.1293690166829784 44-45 0.1199062190432516 46-47 0.1394273750133174 48-49 0.15646702810583246 50-51 0.16493722459453897 52-53 0.17363902801848355 54-55 0.17062813786038866 56-57 0.14187579118583413 58-59 0.13324016117195756 60-61 0.13085791840950886 62-63 0.12715220744569977 64-65 0.11312344451127959 66-67 0.12682134039535964 68-69 0.12476996468325104 70-71 0.1333725079920936 72-73 0.15944483155889336 74-75 0.17224938640705514 76-77 0.17770869273766676 78-79 0.17502866962991198 80-81 0.18065340948569364 82-83 0.1795284615145373 84-85 0.15825371017766898 86-87 0.15937865814882532 88-89 0.17903216093902716 90-91 0.26694353621439126 92-93 0.5275013383572187 94-95 0.17823808001821093 96-97 0.1913073285066448 98-99 0.20831389489412586 100-101 0.21628779080732222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1511181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.75419282573956 #Duplication Level Percentage of deduplicated Percentage of total 1 78.69533363148507 45.44985473038696 2 12.239546553785106 14.137702635338423 3 4.023033495608523 6.970411567493512 4 1.8153697244521232 4.193808524640706 5 0.9675099387130067 2.7938877780625226 6 0.5387079662079569 1.86675862542818 7 0.36535477674548655 1.4770539158174703 8 0.24651744388959948 1.1389932791446689 9 0.16959763213793094 0.8815476914354617 >10 0.8518367224297487 8.644321406816147 >50 0.05247513407313876 2.060256384977352 >100 0.028171141019169742 2.8451352039350937 >500 0.0025715797851834994 1.0120254754867746 >1k 0.0032729197265971813 3.4897360352436686 >5k 5.844499511780681E-4 2.344245523065722 >10k+ 1.1688999023561361E-4 0.694261222727369 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10284 0.6805273491395141 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9106 0.6025750720793869 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7140 0.4724781478856603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6711 0.4440897549664799 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6628 0.4385973619308342 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5140 0.340131327749621 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3481 0.2303496404467764 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3358 0.22221031100841 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3091 0.20454201052024873 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2966 0.19627033426174628 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2919 0.19316018398854937 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2684 0.17760943262256473 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2434 0.1610660801055598 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2407 0.1592793980337233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2299 0.15213266974637718 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1928 0.12758233461114188 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1685 0.11150219596461311 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1665 0.11017872776325272 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1586 0.10495102836787916 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1550 0.10256878560543047 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1524 0.10084827694366194 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.617341006801965E-5 0.0 10-11 0.0 0.0 0.0 1.323468201360393E-4 0.0 12-13 0.0 0.0 0.0 3.639537553741081E-4 0.0 14-15 0.0 0.0 0.0 4.6321387047613754E-4 0.0 16-17 0.0 0.0 0.0 6.617341006801965E-4 0.0 18-19 0.0 0.0 0.0 9.595144459862849E-4 0.0 20-21 0.0 0.0 0.0 0.001356554906394403 0.0 22-23 0.0 0.0 0.0 0.002812369927890835 0.0 24-25 0.0 0.0 0.0 0.004301271654421277 3.3086705034009826E-5 26-27 0.0 0.0 0.0 0.00559165315074766 6.617341006801965E-5 28-29 0.0 0.0 0.0 0.008470196488706516 6.617341006801965E-5 30-31 0.0 0.0 0.0 0.017734473898229266 6.617341006801965E-5 32-33 0.0 0.0 0.0 0.035965248371968676 6.617341006801965E-5 34-35 0.0 0.0 0.0 0.06250078580924456 6.617341006801965E-5 36-37 0.0 0.0 0.0 0.10141075092924011 6.617341006801965E-5 38-39 0.0 0.0 0.0 0.15659937492596848 6.617341006801965E-5 40-41 0.0 0.0 0.0 0.21903398732514504 6.617341006801965E-5 42-43 0.0 0.0 0.0 0.28414862283207637 6.617341006801965E-5 44-45 0.0 0.0 0.0 0.3565754201515239 6.617341006801965E-5 46-47 0.0 0.0 0.0 0.45166661041926814 6.617341006801965E-5 48-49 0.0 0.0 0.0 0.5594300087150381 6.617341006801965E-5 50-51 0.0 0.0 0.0 0.666664019730264 6.617341006801965E-5 52-53 0.0 0.0 0.0 0.7698283660263066 6.617341006801965E-5 54-55 0.0 0.0 0.0 0.8839774983936404 6.617341006801965E-5 56-57 0.0 0.0 0.0 1.0143060295226052 6.617341006801965E-5 58-59 0.0 0.0 0.0 1.172592826405308 6.617341006801965E-5 60-61 0.0 0.0 0.0 1.3664147444945378 6.617341006801965E-5 62-63 0.0 0.0 0.0 1.5579867666414546 6.617341006801965E-5 64-65 0.0 0.0 0.0 1.7327507426310946 6.617341006801965E-5 66-67 0.0 0.0 0.0 1.9352082907342005 6.617341006801965E-5 68-69 0.0 0.0 0.0 2.1602640583755353 6.617341006801965E-5 70-71 0.0 0.0 0.0 2.4172484963746896 6.617341006801965E-5 72-73 0.0 0.0 0.0 2.693952610574114 6.617341006801965E-5 74-75 0.0 0.0 0.0 2.972476493550409 6.617341006801965E-5 76-77 0.0 0.0 0.0 3.2523900181381316 6.617341006801965E-5 78-79 0.0 0.0 0.0 3.575150825744897 6.617341006801965E-5 80-81 0.0 0.0 0.0 3.9370532054068974 6.617341006801965E-5 82-83 6.617341006801965E-5 0.0 0.0 4.294356533069169 6.617341006801965E-5 84-85 6.617341006801965E-5 0.0 0.0 4.686599421247355 6.617341006801965E-5 86-87 6.617341006801965E-5 0.0 0.0 5.108355650315879 6.617341006801965E-5 88-89 6.617341006801965E-5 0.0 0.0 5.520318214694335 6.617341006801965E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 9565 0.0 29.326227 1 TACATGG 9590 0.0 28.754856 2 GTTACCG 70 0.00842721 27.119825 9 ACATGGG 9845 0.0 26.756601 3 CATGGGA 7545 0.0 25.160872 4 TTTGCGT 345 0.0 23.387487 3 ATGGGAT 2920 0.0 22.592113 5 GAGTACT 7080 0.0 21.954914 12-13 AGTACTT 7375 0.0 21.720284 12-13 ATGGGAG 2570 0.0 20.867493 5 TATCACG 115 0.0037407337 20.636017 2 TACCTGG 875 0.0 20.612432 2 CTTTGCG 400 0.0 20.171707 2 GTACTTT 7960 0.0 19.61815 14-15 CATGGGG 2640 0.0 18.876015 4 TACTTTT 8890 0.0 18.206562 14-15 TTGCGTT 445 0.0 18.13067 4 GTGTAGC 725 0.0 17.675816 1 ACTTTTT 9320 0.0 17.672714 16-17 GCTTATA 1125 0.0 17.29757 1 >>END_MODULE